53 results about "Glycosyltransferase activity" patented technology

The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed.

The invention discloses a cyclodextrin glucosyltransferase mutant for improving AA-2G conversion rate and belongs to the field of genetic engineering and enzyme engineering. According to the cyclodextrin glucosyltransferase mutant, the 228th lysine near the active center of the cyclodextrin glucosyltransferase of thermophilic fat bacillus (Bacillus stearotherm opilus NO2) is mutated into arginine to obtain a mutant K228R, the 367th aspartic acid is mutated into serine to obtain a mutant D367S and amphimutation is carried out on the basis to obtain a mutant D367S/K228R. The mutant can be used for realizing the improvement of the AA-2G conversion rate and has relatively high industrial value.

The present invention provides DNA vaccines that direct the coincident expression of vaccine antigens coincidently with mutant ADP-ribosyltransferase toxins (mARTs), which display reduced, or are devoid of, ADP-ribosyltransferase activity, and methods for vaccinating animals with the same. In particular, the present invention provides DNA vaccines that direct the coincident expression of vaccine antigens and mARTs that are useful for vaccinating against viral, bacterial, parasitic pathogens, autoimmune antigens and transplantation antigens.

The invention discloses a preparation method of protein UGTCs4. The protein UGTCs4 has the croctin acid glycosyl transferase activity. The preparation method comprises the following step of culturing engineering bacteria, so as to obtain the protein UGTCs4, wherein the engineering bacteria is a recombinant bacteria which is obtained by importing a recombinant expression carrier into host bacteria, and the recombinant expression carrier is a recombinant plasmid which is obtained by inserting the expression carrier into an encoding gene of the protein UGTCs4. After proofing by experiments, the preparation method of the protein UGTCs4 has the advantages that the croctin acid can be sequentially glycosylated to form crocin 5 and crocin 3; the important application value is realized for the production of crocin 5 and/or crocin 3, preparation of products with croctin acid glycosyl transferase functions, or catalyzing on glycosylation of croctin acids.

The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase.

Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.

The invention provides method for producing a plant cell or plant with increased phlorizin or phloretin glycosyltransferase activity, the method comprising transformation of a plant cell or plant with a polynucleotide encoding a polypeptide with phloretin glycosyltransferase activity. The invention also provides host cells, plant cells and plants, genetically modified to contain and or express the polynucleotides.

The invention discloses an emodin glycosyltransferase protein FtUGT75R2 and coding gene and application thereof. The protein is one protein of the following a) or b): a) protein consisting of the amino acid sequence shown in SEQ ID NO: 2 in the sequence table; and b) protein which is derived from a) obtained by substitution and/or deletion and/or addition of one or more amino acid residues of theamino acid sequence shown in sequence 2 in the sequence table and has emodin 6-O-glucoside glycosyltransferase activity. According to the emodin glycosyltransferase protein FtUGT75R2, an emodin glycosyltransferase sequence is obtained mainly based on a tartary buckwheat genome, and FtUGT75R2 can catalyze emodin to produce emodin 6-O-glucoside by prokaryotic expression of recombinant protein.

The invention discloses an electrochemical sensing detection method of protein O-GlcNAc glycosyltransferase activity. The electrochemical sensing detection method comprises the following steps that a membrane of substrate polypeptide molecules of OGT is formed on the surface of an electrode; the obtained electrode fixed with the membrane of the polypeptide molecules on the surface and a glycosylation reaction fluid are incubated through OGT catalysis to obtain an electrode fixed with the membrane of the glycosylated polypeptide molecules on the surface; the electrode fixed with the membrane of the polypeptide molecules on the surface and the electrode fixed with the membrane of the glycosylated polypeptide molecules on the surface are sequentially subjected to the treatment in the steps 1-3 as follows: 1, the electrodes are put in a protease reaction fluid for hydrolysis; 2, the electrodes are cleaned with water; 3, a tyrosine residue serves as an electrochemical signal probe, and the electrodes perform electrochemical oxidation reaction; tyrosine residue electrocatalytic oxidized currents in the obtained systems are respectively determined, and OGT activity can be quantitively analyzed according to the obtained catalyzed and oxidized currents. By adopting the method, simple, quick and non-marked OGT activity detection is achieved through an electrochemical biosensor.

A starch-containing food can be improved in its quality by using an enzyme having a glycosyltransferase activity which can covert an a-1,4 bond to an a-1,6 bond and a transglutaminase.

The present invention relates to methods and products for the treatment of any disorder or condition, which is associated with abnormal amount of non-collagenous protein, or abnormal oligomerization or dysfunction of non-collagenous protein, such as adiponectin in the blood circulation and/or tissue of a patient. The treatment comprises that functional form of the non-collagenous protein is adjusted in the blood circulation and/or tissue of the patient substantially to the level it is in the blood circulation and/or tissue of a healthy person, by using lysyl hydroxylase and/or glycosyl-transferase activities of LH3 or other lysyl hydroxylase to modify the non-collagenous protein to HMW or other functional form.

The invention discloses aesculin and fraxin glycosyl transferase protein as well as a coding gene and application thereof. The esculetin and fraxin glycosyl transferase protein provided by the invention is a protein as shown in a) or b): a) a protein consisting of an amino acid sequence as shown in a sequence 2 in a sequence table; b) a protein which is obtained by substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid sequence as shown in the sequence 2 in the sequence table, has the activity of aesculin and/or fraxin glycosyltransferase and is derived from a). Based on related results of high-throughput sequencing of aesculus chinensis, a key enzyme UGTs in the last step of synthesis of fraxin and aesculin is found and identified by using a reverse genetics method, the terminal blank of a coumarin biosynthesis pathway is filled, and glycosyl transferase protein and a coding sequence thereof are provided for further biosynthesis of fraxin and aesculin.

The present invention belongs to the technical field of biology, and relates to a de-fucosylated fully humanized monoclonal antibody for Middle East Respiratory Syndrome Coronavirus (MERS-CoV), an antigen binding fragment, and applications thereof, wherein the de-fucosylated fully humanized monoclonal antibody is mainly characterized in that the de-fucosylated fully humanized monoclonal antibody has an enhanced antibody-dependent cell-mediated cytotoxicity (ADCC) effect, wherein the effect comprises cleaving the cells expressing MERS-CoV S protein. The present invention further relates to a method for making cells express the de-fucosylated MERS-CoV fully humanized monoclonal antibody by inhibiting fucosyltransferase activity. According to the present invention, by using the antibody, the Middle Eastern respiratory syndrome can be clinically prevented or treated.

The invention relates to a saponin metabolic pathway, in particular to glycosyl transferase PpUGT73E5 and application of the glycosyl transferase PpUGT73E5 in synthesis of polyphyllin. The glycosyl transferase is a protein as shown in a1 or a2: a1, a protein with an amino acid sequence as shown in SEQ ID NO: 1; a2, a protein with glycosyl transferase activity, which is formed by substitution and/or deletion and/or addition of one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO: 1. The glycosyltransferase can catalyze glycosylation of steroid sapogenin, and a gene resource is provided for subsequent obtaining of multiple polyphyllin.

The invention provides methods and compositions for the modulation of epicatechin glucosyltransferase activity in plants. Increased expression of epicatechin glucosides, and ultimately anthocyanins and proanthocyanidins, in plants may be used to increase the nutritional value of food plants for both human and animal consumption. Increased proanthocyanidin content also reduces the potential for bloat in animals fed certain forage plants low in condensed tannin content.