32 results about "Middle East respiratory syndrome" patented technology

The invention discloses a monoclonal antibody MERS-4V2 and an encoding gene and application thereof. The monoclonal antibody MERS-4V2 is an IgG antibody and comprises a heavy chain and a light chain;CDR1, CDR2 and CDR3 in a heavy chain variable region in the heavy chain are sequentially the 45-52nd amino acid residues, the 70-77th amino acid residues and the 116-120th amino acid residues from theN terminal in the sequence 1 in a sequence table; CDR1, CDR2 and CDR3 in a light chain variable region in the light chain are sequentially the 45-52nd amino acid residues, the 70-72nd amino acid residues and the 109-119th amino acid residues from the N terminal in the sequence 3 in the sequence table. The invention further discloses application of the IgG antibody in preparing medicine for inhibiting Middle East respiratory syndrome coronaviruses. The antibody has wide application prospects in developing medicine (vaccines) for treating and / or preventing the novel coronavirus MERS-CoV.

The invention relates to a kit for performing gene detection on middle east respiratory syndrome coronaviruses, in particular to a kit for detecting middle east respiratory syndrome viruses UPE and N genes through multiple real-time fluorescent polymerase chain reaction technologies. The kit mainly comprises an RT-PCR solution, a primer probe mixed solution, an RT-PCR enzyme system, DEPC H2O and a packaging box for packaging reagent bottles or tubes in a separated and concentrated mode. Due to the fact that the mode that various fluorescent channels are used for detection respectively is adopted for the kit, a one-step method real-time fluorescent PCR mode is applied, the middle east respiratory syndrome coronaviruses UPE and the N genes can be accurately and fast detected, and the kit can be widely applied to various fields of cloinical early-stage diagnosis, epidemic situation prevention and control, scientific research and the like of a middle east respiratory syndrome.

The invention discloses fluorescent RT-PCR (reverse transcription-polymerase chain reaction) primers and a fluorescent probe for detecting MERS (Middle East Respiratory Syndrome) coronavirus, which comprise a first primer pair, a second primer pair and a probe, wherein the first primer pair, second primer pair and fluorescent probe are a first specific forward / reverse primer pair, a second specific forward / reverse primer pair and a fluorescent probe which are respectively designed for conserved sequences of two different genes of the MERS coronavirus. The invention also discloses a kit and method for detecting the MERS coronavirus. By using the two specific probe pairs and primer which are designed for the conserved sequences of the two different genes of the MERS coronavirus, when the double-target PCR (polymerase chain reaction) detection result is positive, the infection of the MERS coronavirus in the clinical sample can be diagnosed. The method is simple, has the characteristics of high speed, high specificity and high sensitivity, and can be used for auxiliary diagnosis and epidemic situation monitoring of the MERS coronavirus.

The invention discloses application of a traditional Chinese medicine composition to preparation of a drug for resisting the Middle East respiratory syndrome corona-virus (MERS-CoV). The traditional Chinese medicine composition is composed of fructus forsythia, honeysuckle, indigowoad root, rheum officinale, herba pogostemonis, rhizoma dryopteris crassirhizomae, rhodiola rosea and the like. The whole adjustment advantage of the compound traditional Chinese medicine is brought into play, the effects of removing pathogenic factors, relieving symptoms and regulating immunity are organically combined, and clinical experiment results show that the medicine provided by the invention has the effects of resisting the MERS-CoV.

The invention discloses a primer probe set, kit and detection method for detecting SARS-CoV and MERS-CoV based on Recombinase Polymerase Amplification (RPA) detection. The primer probe set for detecting SARS-CoV and MERS-CoV based on the RPA detection comprises primers for nucleotide sequences as shown in SEQ ID NO.1-4 and probes for nucleotide sequences as shown in SEQ ID NO. 5-6. The invention further provides the kit for detecting SARS-CoV and MERS-CoV based on the RPA detection, wherein the kit comprises the primer probe set. By adopting the technical scheme, the sensitivity, specificity and simplicity for detecting SARS-CoV and MERS-CoV are obviously improved.

The invention belongs to the technical field of biological medicines, and particularly relates to application of gallic acid as well as derivatives and structural analogues thereof in preparation of anti-coronavirus drugs. Researches show that gallic acid as well as derivatives and structural analogues thereof have an obvious antivirus effect on coronaviridae viruses including 2019 novel coronaviruses (2019-nCoV or SARS-CoV-2), Middle East respiratory syndrome coronaviruses (MERS-CoV) and the like. Gallic acid substances are natural, widely exist in various plants, are polyphenol compounds existing in the nature, are widely applied to the fields of food, biology and medicine, and are very safe, so that the substances have very good application value in the aspect of preparation and development of antiviral drugs for coronaviridae.

The invention relates to the technical filed of biology, in particular to application of a plant as a host in expressing a vaccine of Middle East Respiratory Syndrome. According to the technical scheme, lettuce is utilized to instantly express the vaccine to deal with Middle East Respiratory Syndrome, and high-content protein can be produced within a short time (4d). The biology safety problem is reduced to the largest limit, the lettuce basically contains no botanical toxic substances, and the fiber of the lettuce itself is little, which is conducive to protein purification in the downstream. As test results show, through the adoption of a lettuce system to produce the vaccine, the production period can be sharply shortened, and the production cost can be sharply reduced.

The invention belongs to the technical field of biology, and relates to fused protein of an antibody and an antivirus peptide, in particular to a bispecific efficient immunotherapeutic agent, of a fully human neutralizing antibody and a high-activity MERS-CoV-inhibiting peptide, for Middle East respiratory syndrome coronaviruses (MERS-CoV), and application of the bispecific efficient immunotherapeutic agent, of the fully human neutralizing antibody and the high-activity MERS-CoV-inhibiting peptide, for the Middle East respiratory syndrome coronaviruses (MERS-CoV). The antibody-peptide bispecific immunotherapeutic agent is the fused protein of the antibody and the antivirus peptide, wherein the antibody is the high-activity fully human neutralizing antibody to the MERS-CoV, and the peptideis the high-activity MERS-CoV-inhibiting peptide. The bispecific immunotherapeutic agent has antivirus activity of the antibody and the antivirus activity of the peptide at the same time, can be usedfor further producing a potent antivirus medicine, and is especially suitable for treating disease caused by the Middle East respiratory syndrome coronaviruses (MERS-CoV).

A peptide that binds to the Si spike protein of Middle East Respiratory syndrome coronavirus or MERS-CoV and a method for inhibiting infection of a subject exposed to or having MERS-CoV by administering the peptide.

The present invention provides monoclonal antibodies that bind to the Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) spike protein, and methods of use. In various embodiments of the invention, the antibodies are fully human antibodies that bind to MERS-CoV spike protein. In some embodiments, the antibodies of the invention are useful for inhibiting or neutralizing MERS-CoV activity, thus providing a means of treating or preventing MERS infection in humans. In some embodiments, the invention provides for a combination of one or more antibodies that bind to the MERS-CoV spike protein for use in treating MERS infection. In certain embodiments, the one or more antibodies bind to distinct non-competing epitopes comprised in the receptor binding domain of the MERS-CoV spike protein.

The present invention provides monoclonal antibodies that bind to the Middle East Respiratory Syndrome--Coronavirus (MERS-CoV) spike protein, and methods of use. In various embodiments of the invention, the antibodies are fully human antibodies that bind to MERS-CoV spike protein. In some embodiments, the antibodies of the invention are useful for inhibiting or neutralizing MERS-CoV activity, thus providing a means of treating or preventing MERS infection in humans. In some embodiments, the invention provides for a combination of one or more antibodies that bind to the MERS-CoV spike protein for use in treating MERS infection. In certain embodiments, the one or more antibodies bind to distinct non-competing epitopes comprised in the receptor binding domain of the MERS-CoV spike protein.

The invention belongs to the technical field of biological medicines, and particularly relates to application of gallic acid and derivatives and structural analogs thereof in preparation of anti-coronavirus medicines. Researches of the invention show that gallic acid and derivatives and structural analogs thereof have obvious antiviral effects on various coronaviridae viruses, including 2019-novelcoronaviruses (2019-nCoV or SARS-CoV-2), Middle East Respiratory Syndrome-Coronavirus (MERS-CoV), etc. Moreover, gallic acid and derivatives and structural analogs thereof are natural in source, widely exist in various plants, can be widely applied to the fields of food, biology and medicines, and are very safe, so that gallic acid and derivatives and structural analogs thereof have very good application value in the aspect of preparing and developing antiviral drugs against coronaviridae.

The invention belongs to the field of biomedicine, and relates to a compound shown in a formula I, stereoisomers, mixtures or pharmaceutically-acceptable salts of the compound. The invention further relates to an application of the compound, stereoisomers, mixtures or pharmaceutically-acceptable salts thereof in preparing medicines for treating and / or preventing MERS-CoV infection or diseases related to MERS-CoV infection (such as Middle East respiratory syndrome). The formula is as below:(Z-Ile-Glu-X1-Ile-Val-Lys-Lys-Ile-X2-X3-Ile-Glu-Arg-Ala-Ile-Glu-Ala-Gln-Gln-X4-Leu-Leu-Gln-Leu-Thr-Val-Trp-X5-Ile-Lys-Gln-Leu-Gln-Ala-Arg-Ile-Leu-B)n(I).

The invention relates to application of a Shuanghuanglian preparation in virus infection resistance. Specifically, the invention provides an application of a chlorogenic acid compound or a pharmaceutically acceptable salt or an extract of the chlorogenic acid compound. Experiments show that the Shuanghuanglian preparation (oral liquid) and the active ingredient chlorogenic acid thereof have obvious antiviral effects on severe acute respiratory syndrome coronavirus (SARS-CoV), influenza virus (influenza virus), Middle East Respiratory Syndrome (MERS) and 2019-nCoV (new coronavirus), and prove that the Chinese patent medicine Shuanghuanglian preparation (oral liquid) can be used for treating virus infectious diseases.

Systems and methods for assessing a disease are provided. Medical imaging data of lungs of a patient is received. The lungs are segmented from the medical imaging data and abnormality regions associated with a disease are segmented from the medical imaging data. An assessment of the disease is determined based on the segmented lungs and the segmented abnormality regions. The disease may be COVID-19 (coronavirus disease 2019) or diseases, such as, e.g., SARS (severe acute respiratory syndrome), MERS (Middle East respiratory syndrome), or other types of viral and non-viral pneumonia.

The invention discloses a middle east and respiratory syndrome coronavirus antibody and a preparation method thereof, and belongs to the technical field of cell-mediated immunity. RDB protein of an MERS-CoV virus S protein receptor binding domain is obtained by using in-vitro expression and purification of a baculovirus expression vector system, and Bab / c mice are immunized by using high-purity MERS RBD protein, an efficient antiviral neutralizing anti-body is screened by the methods such as an enzyme-linked immuno sorbent assay (ELISA), a false virus neutralization experiment and the like. The antibody and the crystal structure of the MERS RBD protein are analyzed, and a crucial amino acid of the antibody combined with viral proteins is determined from the molecular level. Thus, the antibody is further humanized, and the possibility is provided for clinical detection, prevention and treatment of MERS-CoV virus infection.

Provided is an application of a plant as a host in expressing a vaccine of Middle East respiratory syndrome. In particular, lettuce is utilized to transiently express fusion proteins to prepare a vaccine for Middle East respiratory syndrome.

The invention relates to a traditional Chinese medicine for treating Middle East respiratory syndrome. The traditional Chinese medicine is prepared from the following traditional Chinese medicines in parts by mass: 10 parts of honeysuckle, 10 parts of fructus forsythiae, 10 parts of scutellaria baicalensis, 10 parts of schizonepeta, 10 parts of radix sileris, 5 parts of ginger, 10 parts of bupleurum falcatum, 6 parts of mint, 10 parts of ligusticum wallichii, 10 parts of tuckahoe, 10 parts of radix angelicae pubescentis, 3 parts of ephedra, 10 parts of red dates, 10 parts of adenophora stricta, 10 parts of white mulberry root-bark, and 10 parts of blackend swallowwort root.

The present invention provides systems and methods for assessing a disease. Medical imaging data of lungs of a patient is received. The lungs are segmented from the medical imaging data and abnormality regions associated with a disease are segmented from the medical imaging data. An assessment of the disease is determined based on the segmented lungs and the segmented abnormality regions. The disease may be COVID-19 (coronavirus disease 2019) or diseases, such as, e.g., SARS (severe acute respiratory syndrome), MERS (Middle East respiratory syndrome), or other types of viral and non-viral pneumonia.

The invention discloses application of a novel compound in preparation of a medicine for preventing and / or treating coronavirus infection. The coronavirus targeted by the invention at least comprises SARS-CoV-2, SARS-CoV and MERS-CoV. In-vitro test results show that the novel compound, including drugs prepared from a compound III and a compound IV, have an obvious effect of resisting coronavirus infection; the medicine can be applied to prevention and / or treatment of novel coronavirus pneumonia, severe acute respiratory syndrome, middle east respiratory syndrome and other diseases caused by SARS-CoV-2, SARS-CoV, MERS-CoV and other coronaviruses. The compound III and the compound IV are high in activity and good in antiviral effect. The compound is low in preparation cost, simple in preparation method, suitable for large-scale production and convenient for later clinical popularization and application.

The invention relates to the technical field of biology and discloses a single-domain heavy-chain antibody, an encoding gene, a preparation method and use thereof and a pharmaceutical composition. The single-domain heavy-chain antibody can be specifically combined with a receptor binding region of the Middle East Respiratory Syndrome Coronavirus, has good neutralizing activity on the Middle East Respiratory Syndrome Coronavirus, and has the potential as an active component in an antiviral product for preventing and treating the Middle East Respiratory Syndrome.

The present invention relates to a binding molecule having neutralizing activity against Middle East Respiratory Syndrome-Coronavirus (MERS-CoV). More particularly, the present invention relates to a binding molecule having strong ability to bind to an S protein of MERS-CoV and neutralizing activity against MERS-CoV and thus being very useful in the prevention, treatment or diagnosis of MERS-CoV infection.

The present disclosure relates to 2, 4, 7-substituted-7-denitrification-2 '-deoxy-2'-fluoro arabinosyl nucleosides and nucleotide prodrugs capable of inhibiting viral infections and methods of using these compounds in the treatment of viral infections, the viral infections such as human immunodeficiency virus (HIV), human papilloma virus (HPV), herpes simplex virus (HSV), human cytomegalovirus (HCMV), varicella, infectious mononuclear hyperplasia, parotitis, measles, rubella, herpes zoster, Ebola virus, viral gastroenteritis, viral hepatitis, viral meningitis, human metapneumovirus, human parainfluenza virus type 1, parainfluenza virus type 2, and the like. Parainfluenza virus type 3, respiratory syncytial virus, viral pneumonia, chikungunya virus (CHIKV), Venezuelan equine encephalitis (VEEV), dengue fever (DENV), influenza, West Nile virus (WNV), zika virus (ZIKV), 229E, NL63, OC43, HKU1, Middle East Respiratory Syndrome Coronavivirus (MERS), severely acute respiratory Syndrome Coronavivirus (SERS), and severely acute respiratory Syndrome Coronavivirus (SERS) This abstract is intended as a scanning tool for search purposes in the particular art and is not intended as a limitation on the present invention.

The invention relates to a manufacturing method of inorganic powder, which uses soluble natural inorganic salt with an antibacterial function, and comprises the following steps: preparing materials, dissolving the materials, feeding the materials, sampling and analyzing the powder, and ageing and spray-drying a product to finally prepare the inorganic powder. The method is simple and is low in themanufacturing cost, wherein the manufactured inorganic powder has antibacterial broad spectrum and is safe to human and livestock environments, the inorganic powder can be doped into daily supplies to effectively kill various bacteria or prevent and control various viruses including atypical respiratory syndrome, Middle East respiratory syndrome, African swine fever and the like for a long time,and physical and psychological health and social stability of people can be guaranteed.

The present invention belongs to the field of biomedicine, and relates to compounds represented by formula I, stereoisomers, mixtures thereof, or pharmaceutically acceptable salts thereof. The present invention also relates to the use of said compound, its stereoisomer, mixture, or pharmaceutically acceptable salt in the preparation of treatment and / or prevention of MERS-CoV infection or diseases associated with MERS-CoV infection (such as Middle East Respiratory Syndrome) use in medicines. (Z-Ile-Glu-X 1 ‑Ile‑Val‑Lys‑Lys‑Ile‑X 2 -X 3 ‑Ile‑Glu‑Arg‑Ala‑Ile‑Glu‑Ala‑Gln‑Gln‑X 4 ‑Leu‑Leu‑Gln‑Leu‑Thr‑Val‑Trp‑X 5 ‑Ile‑Lys‑Gln‑Leu‑Gln‑Ala‑Arg‑Ile‑Leu‑B)n (I).

The invention discloses an MERS freeze-drying tube for detecting Middle East Respiratory Syndrome Virus, a preparation method of the MERS freeze-drying tube and a freeze-drying kit. The freeze-drying kit for detecting the Middle East Respiratory Syndrome Virus (MERS) comprises an MERS freeze-drying tube, an MERS positive control and an MERS negative control, and the MERS freeze-drying tube comprises Taq enzyme, M-MLV reverse transcriptase, an RNA enzyme inhibitor, Buffer, a primer probe mixture, dNTP, MgCl2 and a freeze-drying protective additive; the freeze-drying protective agent comprises the following components in percentage by weight: 3%-8% of trehalose, 1%-5% of mannitol, 0.1%-2% of BSA (Bovine Serum Albumin), 1%-3% of lactose and 0.05%-0.2% of tween80. The MERS positive control comprises a plasmid with a target gene. The enzyme, the buffer, the primer probe, the dNTP, the freeze-drying protective agent and the like are fully premixed to form a freeze-drying reaction system to form the freeze-drying kit, and the freeze-drying kit can be stably stored at 4-25 DEG C. Therefore, the Middle East Respiratory Syndrome Coronavirus in the sample can be rapidly, efficiently and qualitatively detected, the reaction is totally closed, and only one sample adding operation is performed; the invention relates to a freeze-drying kit which can be stored and transported at normal temperature.

The invention discloses fluorescent RT-PCR (reverse transcription-polymerase chain reaction) primers and a fluorescent probe for detecting MERS (Middle East Respiratory Syndrome) coronavirus, which comprise a first primer pair, a second primer pair and a probe, wherein the first primer pair, second primer pair and fluorescent probe are a first specific forward / reverse primer pair, a second specific forward / reverse primer pair and a fluorescent probe which are respectively designed for conserved sequences of two different genes of the MERS coronavirus. The invention also discloses a kit and method for detecting the MERS coronavirus. By using the two specific probe pairs and primer which are designed for the conserved sequences of the two different genes of the MERS coronavirus, when the double-target PCR (polymerase chain reaction) detection result is positive, the infection of the MERS coronavirus in the clinical sample can be diagnosed. The method is simple, has the characteristics of high speed, high specificity and high sensitivity, and can be used for auxiliary diagnosis and epidemic situation monitoring of the MERS coronavirus.