60results about How to "Has antiviral activity" patented technology

The invention relates to a gallbladder wood leaf composition containing strictosamide. The gallbladder wood leaf composition is characterized in that the gallbladder wood leaf composition is prepared by combining alkaloid and flavonoid extracted from gallbladder wood leaf; the alkaloid contains strictosamide,10-hydroxy-strictosamide and short snakeroot glucoside; the flavonoid contains kaempferol-3-O-rutinoside and rutin; and the total weight of the three alkaloid and flavonoid is 50-98% of that of the composition, and the ratio of the total weight of the three alkaloid and the total weight of the flavonoid is (10-0.7):(0.5-10). The invention also discloses a preparation of the composition. The composition provided by the invention can play the role of clearing away heat and toxic materials and relieving swelling and pain, and can treat diseases such as acute tonsillitis, acute pharyngitis, acute conjunctivitis, upper respiratory tract infection or chronic pelvic inflammatory disease and appendagitis; and can cure slight and medium pain.

The invention belongs to the technical field of biology, and relates to fused protein of an antibody and an antivirus peptide, in particular to a bispecific efficient immunotherapeutic agent, of a fully human neutralizing antibody and a high-activity MERS-CoV-inhibiting peptide, for Middle East respiratory syndrome coronaviruses (MERS-CoV), and application of the bispecific efficient immunotherapeutic agent, of the fully human neutralizing antibody and the high-activity MERS-CoV-inhibiting peptide, for the Middle East respiratory syndrome coronaviruses (MERS-CoV). The antibody-peptide bispecific immunotherapeutic agent is the fused protein of the antibody and the antivirus peptide, wherein the antibody is the high-activity fully human neutralizing antibody to the MERS-CoV, and the peptideis the high-activity MERS-CoV-inhibiting peptide. The bispecific immunotherapeutic agent has antivirus activity of the antibody and the antivirus activity of the peptide at the same time, can be usedfor further producing a potent antivirus medicine, and is especially suitable for treating disease caused by the Middle East respiratory syndrome coronaviruses (MERS-CoV).

The invention provides alpha-pyrone compounds having antiviral activity and their application in resisting influenza viruses H1N1 and H3N2. The invention also discloses a preparation method of the alpha-pyrone compounds having antiviral activity, comprising: (1) acquiring a fermented material having rich pyrone compounds through microbial fermentation culture, wherein a recombinant vector used inthe microbial fermentation culture carries polyketide synthase having a nucleotide sequence of KU534995.1; (2) separating and purifying the fermented material of step (1) to obtain the alpha-pyrone compounds. Compared with existing drugs, the pyrone compounds have good anti-H2N1 and anti-H3N2 activity and are substantially nontoxic to normal cells; compared with existing drugs, the alpha-pyrone compounds have natural advantages, including good activity and toxic and side effects reduced as far as possible, and also have a promising application prospect and huge market.

The invention discloses an alga oligosaccharide fish feed additive and relates to an alga oligosaccharide. Aiming at solving the problems of the prior art, the invention provides the alga oligosaccharide fish feed additive. The alga oligosaccharide fish feed additive improves a feed utilization rate, promotes growth, improves disease resistance, reduces a cost and improves fish quality. The alga oligosaccharide of the alga oligosaccharide fish feed additive is gracilaria lemaneiformis agar oligosaccharide and is a mixture which comprises tetrasccharide to octasaccharide and is obtained through co-culture of a Flammeovirga Pacifica H2 strain and gracilaria lemaneiformis so that gracilaria lemaneiformis is directly degraded through an enzyme produced through microorganism. The Flammeovirga Pacifica H2 has a preservation number of CCTCC NO: M2012229.

The invention discloses a preparation method of colorful crisp potato chips through osmotic dehydration and vacuum freeze-drying. According to the preparation method, an osmotic dehydration technology and a vacuum freeze-drying technology are combined, a sucrose solution is firstly taken as an osmotic solution to remove part of moisture in the colorful potato chips, and then the colorful crisp potato chips are prepared with the vacuum freeze-drying technology. According to the scheme, the drying temperature is low, the drying time is shorter than that of single vacuum freeze-drying, the prepared colorful crisp potato chips have good color, luster and shape and taste crisp and slightly sweet, and nutrient substances of the prepared colorful crisp potato chips are almost not lost; besides, with the adoption of the method, anthocyanin in the colorful potato chips can be reserved to the greatest extent, so that the prepared colorful crisp potato chips have certain functions of resisting to oxidation, eliminating free radicals and delaying senescence; further, the colorful crisp potato chips have functions of protecting the liver and preventing cardiovascular diseases as well as certain anti-mutation effect, antitumor activity and antiviral activity.

The invention relates to a natural film coating agent applied to food and healthcare products. The natural film coating agent comprises the following raw materials in parts by weight: 40-67 parts of cellulose ether derivatives, 3-32 parts of seaweed polysaccharides, 1-10 parts of natural pigments, 2-25 parts of micromolecular sugar alcohol and 4-15 parts of lecithin. According to the coating agent, natural plant materials are adopted, pollution of heavy metal lead and arsenic is very low and cannot directly influence the coating agent, and the actually measured heavy metal lead and arsenic content also meets the regulations and standards of food additives; the coating agent does not contain chemical synthesized materials and is free from harmful compounds and monomer residues, so that the requirements of the food and the healthcare products on the safety are fully met; main ingredients in the natural coating agent can be dissolved in water, so that an organic solvent does not need to be used in a coating process and the production cost is greatly saved; a water solution is used for performing a coating process, so that coated drugs do not contain organic solvent residues and the safety of the drugs is improved.

The invention provides an antiviral water-based matrix emulsion composition and an antiviral coating containing the same. The emulsion composition is composed of an inorganic metal nanocomposite dispersion liquid with photocatalytic antiviral activity, a water-based high-molecular polymer emulsion containing a high-content quaternary phosphonium salt unit, and an optional polyvinyl alcohol auxiliary agent. The water-based matrix emulsion or the coating thereof can be used for surface coating treatment of textile articles, plastic products, glass products, leather, wooden products and other objects, so that the virus activity on the surfaces of the objects is reduced or inactivated in a short time, and the effects of inhibiting viruses and blocking propagation are achieved.

The invention relates to a method for preparing carrageenin-sulfate oligosaccharide with antiviral activity, which is characterized in that culture medium for beta-glucanase production is prepared, marine cellulophaga is inoculated; kappa-carrageenin crude digestive enzyme liquid is fermented and extracted; additionally, the kappa-carrageenin is dissolved in distilled water and undergoes enzymatic hydrolysis reaction after the kappa-carrageenin crude digestive enzyme liquid is added; the enzymatic hydrolysis reaction liquid is converted into concentrated liquid after the rotary evaporation; enzymatic hydrolysis oligosaccharide fragment is collected by precipitating organic solvent; and then sulfate modification is carried out on the enzymatic hydrolysis oligosaccharide fragment and carrageenin-sulfate oligosaccharide is obtained. The invention has the advantage that the step combining enzymatic hydrolysis and the sulfate modification is applied, so that the preparation process is simple, the product yield is high, the product quality is stable and active groups during the preparation are not damaged, in addition, the product has the antiviral activity, in particular to the obvious antiviral activity against herpes simplex virus.

The invention relates to a recombinant strain of a mink IFN-Gamma gene, which is characterized by being an IFN-Gamma gene sequence which is cloned from peripheral mink bloodlymph cells (PMBC) simulated by phytohemagglutinin (PHA) and being a recombinant plasmid vector containing a nucleotide sequence; i.e., using a plasmid vector pGM-T which is formed by the cutting of a cloning vector by taking an EcoR V enzyme as the cutting point and then adding a 'T' from the '3' end at both sides; and TA cloning is carried out on the nucleotide sequence of a coding Gamma-interferon which is amplified from the peripheral mink bloodlymph cells so as to construct the recombinant plasmid of the nucleotide sequence of the mink Gamma-interferon. In addition, Escherichia coli conversed from the plasmid vector is used as a host. The recombinant strain aims at laying down foundation for researching and developing genetically engineered mink recombinant interferons biological products with anti-viral activity and immunomodulatory activity.

The invention discloses a group of thiourea compounds and a preparation method and application thereof. The oxazole phenylthiourea compound or a pharmaceutically acceptable salt thereof has a structure represented by the general formula (I). According to the present inventors, a class of compounds having both IMPDH inhibitory activity and broad-spectrum antiviral activity and pharmaceutical saltsthereof are screened and obtained by studying the structure-activity relationship and the action mechanism of active compounds, wherein the virus comprises influenza virus, hepatitis B virus, coxsackie virus and herpes simplex virus. The invention lays a foundation for the development and application of the compounds as antiviral and other related drugs and pharmaceutical compositions thereof, andprovides a new technical means for antiviral treatment.

A kind of antineoplastic antibiotic compound, chinikornycin A and B, for suppressing the cells of lung cancer, breast cancer, melanoma, renal cancer and uterine cancer is prepared from Streptomyces sp. through fermenting and chromatography by silica gel column.

The invention discloses an application of N-acetyl-L-cysteine in preparing a drug for treating or preventing porcine virus infection. The application comprises the steps: A, preparing N-acetyl-L-cysteine, absolute ethyl alcohol, tween-80, lactose, soluble starch and bran into a granule, oral liquid or powder, B, adding the granule, the oral liquid or the powder into feed or drinking water, or C, independently administering N-acetyl-L-cysteine, D, continuously using N-acetyl-L-cysteine during a whole feeding period as preventive addition and long-term usage in the feed, E, feeding the powder and the granule directly for treatment, or F, mixing the powder and the granule with daily ration, adding into the feed in the morning, at noon and in the evening every day, and continuously using the powder and the granule. N-acetyl-L-cysteine has high safety, no toxic or side effects, and antiviral activity, and can effectively prevent and treat the porcine circovirus type 2 and porcine epidemic diarrhea virus infection, reduce a mortality rate of infected pigs, and effectively increase a survival rate and productivity of the pigs.

The invention discloses a method for synthesizing marine polyhydroxyl sterol (25R)-5alpha-cholest-3beta, 5alpha, 6beta, 26-tetrol. Diosgenine is used as a raw material, and (25R)-5alpha-cholest-3beta, 5alpha, 6beta, and 26-tetrol are prepared by the steps of reduction ring-opening carried out by zinc amalgam, 3, 26-bit hydroxy protection carried out by tert-butyl dimethylchlorosilane, 16-bit hydroxyl esterification carried out by methylsufonyl chloride and sulfuryl, 16-bit methyl sulphonic acid ester reduction carried out by lithium aluminum hydride, 5-6-bit double linkage oxidation carried out by m-chloroperoxybenzoic acid, and ring opening and deprotection reaction under acid condition. The synthesizing method has the advantages of low-cost and easily obtained raw materials, moderate reaction condition, favorable selectivity and high yield, and the target compound has the potential antineoplastic and antiviral activity.

The invention relates to a traditional Chinese medicine composition for treating chronic prostatitis and a preparation method thereof. The traditional Chinese medicine composition is prepared from thefollowing raw materials in parts by weight: 8 to 12 parts of cortex eucommiae chlorogenic acid, 6 to 10 parts of cortex cinnamomi polyphenol, 13 to 17 parts of oyster peptide, 8 to 12 parts of maca,8 to 12 parts of cordyceps militaris, 13 to 17 parts of silk peptide, 2 to 6 parts of herba abelmoschi esculenti, 2 to 6 parts of cortex pausinystaliae macroceras, 2 to 6 parts of catuaba, 2 to 6 parts of herba epimedii, 2 to 6 parts of herba gynostemmatis, 2 to 6 parts of herba rhodiolae, 2 to 6 parts of radix salviae miltiorrhizae and 2 to 6 parts of semen persicae. The traditional Chinese medicine composition disclosed by the invention is simple in preparation and remarkable in curative effect, has no side effect, is safe and effective, is low in cost and is suitable for oral administration.

The invention provides a method for preparing porcine alpha-interferon, which comprises: obtaining the coding region of a porcine alpha-interferon gene by the conventional polymerase chain reaction (PCR) process; cloning the coding region to a Pichia pastoris secretory expression vector pHBM905A; performing electric transformation of Pichia pastoris; and screening a high-resistance transformant to realize the secretory expression of the recombinant protein in the Pichia pastoris and obtaining the recombinant protein with antiviral activity. In the invention, by optimizing the expression system of the Pichia pastoris, the antiviral activity of the recombinant protein is improved.

The invention relates to application of an exogenous ATG10S protein in preparation of an antiviral drug. The exogenous ATG10S is an rhATG10S protein, the coding nucleotide sequence of the exogenous ATG10S is shown as SEQ ID NO. 1 or SEQ ID NO. 2, and the amino acid sequence of the rhATG10S protein is shown as SEQ ID NO. 3. The invention also relates to application of the rhATG10S protein in preparation of drugs for inhibiting viruses. The invention further discloses a method for screening antiviral drugs by taking the ATG10S protein as a target spot.

The invention discloses lipopeptide compounds Totopotensamides and a preparation method and application thereof. The invention discloses antibiotic compounds Totopotensamides (TPMs) R1-1 to R1-6(1-6),R2-1(7), P3-1(8), P3-2(9), P2-1 to P2-7(10-16), U5-1(17), U5-2(18), H1 to H8(19-26) and HM1 to HM3(27-29). The lipopeptide compounds Totopotensamides with antiviral activity are obtained through separation and purification from mutant strains, especially the lipopeptide compounds Totopotensamides have inhibitory activity on 3CL hydrolase of a novel coronavirus and are expected to be used for preparing anti-novel-coronavirus drugs.

The invention discloses a novel beta-furan phosphonate purine nucleoside analog, and in particular relates to a (2R,5R)-5-phosphoryl methoxy-2-(2-substituted adenine-9-yl)-2,5-dihydrofuran nucleoside analog as well as a preparation method and an application thereof, which belongs to the field of nucleoside chemicals and pharmaceutical chemistry. The analog has a structure as shown in the formula (1) in the specification, wherein R1 represents C1, NH2, OCH3, SCH3, NHCH3 or NHNH2; R2 represents NH2, OCH3 and NHCH3; R' represents H, Na or K. Chlorinated sugar with high activity is formed through chlorination of 3.5-di-O-p-methyl benzoyl-2-deoxidation-D-ribose glucoside, the chlorinated sugar is subsequently reacted with alkali, and a novel beta-D-dihydrofuran phosphonate adenine nucleoside analog is formed through selective oxidation, decarboxylation, addition, elimination, functional group conversion and ethyl removal. The analog has anti-virus activity and good development prospect.

The invention discloses a preparation method of cordyceps militaris craft beer. The preparation method comprises the following steps: preparation of a cordyceps militaris extract, gelatinization, saccharification, filtration, clarification, tank washing, boiling, wort cooling, fermentation and wine filtration. The prepared beer is clear and transparent in color, rich in foam and pure and natural in taste, has the characteristic flavor of beer, also has a good human body health-care effect, and meets the double requirements of consumers on taste and health.

The invention relates to a recombinant expression and application of a reconstructed fenneropenaeus chinensiss antifungal protein gene ALFm. Reconstruction is carried out with a fenneropenaeus chinensiss anti-lipopolysaccharide factor FcALF2 as an archetype, reconstructed antifungal protein is named ALFm, and has a base sequence shown in SEQ ID No.1 and an amino acid sequence shown by SEQ ID No.2. An expression vector pPIC9K and expression strain pichia pastoris GS115 are utilized for carrying out eukaryon recombinant expressions on ALFm, and recombinant protein with biological activity is obtained. The biological function of ALFm is verified in vitro, and it is proved that the recombinant protein can have effective inhibiting effects on various bacteria and WSSV.

The invention relates to a compound, and a preparation method and an application thereof. The compound is prepared by connecting sydnone with a PBTs compound. A pharmaceutical composition containing the compound and/or the pharmacologically acceptable salt thereof has the following advantages: the anticancer effect is obvious; compared with (-)-Antofine, the compounds have better anticancer effects, have no obvious toxicity to animals, also have antiviral activity, and can be used for preparing drugs for preventing and/or treating cancers and/or resisting viruses.