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Recombinant expression and application of reconstructed fenneropenaeus chinensiss antifungal protein gene ALFm

A technology of antibacterial protein and prawn, applied in the field of genetic engineering, can solve the problems of low yield and high cost of small peptides, and achieve far-reaching effects

Inactive Publication Date: 2016-01-20
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cost of using chemical methods to synthesize small peptides is high and the yield is low

Method used

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  • Recombinant expression and application of reconstructed fenneropenaeus chinensiss antifungal protein gene ALFm
  • Recombinant expression and application of reconstructed fenneropenaeus chinensiss antifungal protein gene ALFm
  • Recombinant expression and application of reconstructed fenneropenaeus chinensiss antifungal protein gene ALFm

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Embodiment

[0039] The modified antibacterial protein gene ALFm of Penaeus chinensis has the following sequence:

[0040] (1) Information of SEQIDNo.1 (see sequence listing)

[0041] (a) Sequential features

[0042] * Length: 327 bp

[0043] *Type: nucleic acid

[0044] * Chain type: double chain

[0045] *Topology: Linear

[0046] (b) Molecular type: cDNA

[0047] (c) Assumption: No

[0048] (d) Antisense: No

[0049] (e) Original source: Chinese prawn (Fenneropenaeuschinensis)

[0050] Sequence description: SEQIDNo.1

[0051] GATGATGATGATAAGTCTGGTTGGGAAGCTCTGGTTCCAGCTATTGCTGATAAGTTGACTGGTTTGTGGGAATCTGGTGAATTGGAATTGTTGGGTCATTACTGTAAGTTTAAGGTTAAGCCAAAGTTTAAGAGATGGAAGTTGAAGTTTAAGGGTAGAATGTGGTGTCCAGGTTGGACTACTATTGGTGGTCAAGCTGAAACTAGATCTAGATCTGGTGTTGTTGGTAGAACTACTCAAGATTTTGTTAGAAAGGCTTTTAGAGCTGGTTTGATTACCGAATCTGAAGCTCAAGCTTGGTTGAACAAC

[0052] CATCATCATCATCATCAT

[0053] (2) Information of SEQ ID No.2

[0054] (a) Sequential features

[0055] * Length: 109 amino acid residues

[0...

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Abstract

The invention relates to a recombinant expression and application of a reconstructed fenneropenaeus chinensiss antifungal protein gene ALFm. Reconstruction is carried out with a fenneropenaeus chinensiss anti-lipopolysaccharide factor FcALF2 as an archetype, reconstructed antifungal protein is named ALFm, and has a base sequence shown in SEQ ID No.1 and an amino acid sequence shown by SEQ ID No.2. An expression vector pPIC9K and expression strain pichia pastoris GS115 are utilized for carrying out eukaryon recombinant expressions on ALFm, and recombinant protein with biological activity is obtained. The biological function of ALFm is verified in vitro, and it is proved that the recombinant protein can have effective inhibiting effects on various bacteria and WSSV.

Description

technical field [0001] The invention relates to genetic engineering, in particular to the recombinant expression and application of a modified Chinese prawn antibacterial protein gene ALFm. Background technique [0002] Shrimp farming is one of the pillar industries of mariculture. With the increasing market demand for high-quality aquatic products, shrimp farming occupies an increasingly important position in aquaculture. However, the explosive prevalence of shrimp diseases has caused huge losses to shrimp farming, and the frequent occurrence of shrimp diseases caused by various bacterial viruses has plagued the development of shrimp farming. At present, antibiotics, hormones and other drugs in aquaculture, although they play a certain role in disease control, they also destroy the micro-ecological balance of the water environment and animals, causing aquatic animals to lose their normal flora barrier and biological antagonism. At the same time, the abuse of antibiotics wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435C12N15/81A61K38/17A61P31/04A61P31/20
Inventor 李富花杨辉李诗豪相建海
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI