Method for preparing and expressing antibacterial peptide gene

Abstract

The invention relates to an antibacterial peptide gene and a preparation method thereof; the antibacterial peptide gene provided by the invention is ascaris suum antibacterial peptide Cecropin P2; and coded ascaris suum antibacterial peptide Cecropin P2 is firstly separated from mammals in vivo. The antibacterial peptide gene provided by the invention is a gene Cecropin P2' obtained by optimizing the ascaris suum antibacterial peptide Cecropin P2; a sequence of the antibacterial peptide gene is SEWNo1; in the sequence, a sixth codon is changed from original ctc into ctg in an optimization method; a ninth codon, a twelfth codon and an eighteenth codon are changed from original aaa into aag in an optimization method; by dint of above-mentioned optimization, a target gene can be expressed preferably in a pichia pastoris system.

Description

technical field [0001] The invention relates to an antibacterial peptide gene and a preparation method thereof. Background technique [0002] In recent years, the extensive abuse of antibiotics and chemical drugs in the breeding industry has led to the continuous emergence of bacterial resistance, and some infectious diseases have seriously threatened human health and the development of animal husbandry. Residues of chemical drugs in meat products have laid a huge hidden danger for human health. Therefore, it is imminent to find new biological agents that can replace antibiotics and chemical drugs. Antimicrobial peptides (antimicrobial peptides or antibacterial peptides) are a class of active peptide substances with antibacterial activity, and are an important part of the natural defense system in organisms. Good, good water solubility, not easy to produce drug resistance and so on. At the same time, antimicrobial peptides have broad-spectrum antibacterial activity, showi...

AI Technical Summary

Problems solved by technology

However, since the prokaryotic expression system does not have the expression regulation mechanism similar to eukaryotes and the function of post-translational processing and modification of proteins, its products often form inclusion bodies, which must undergo a series of treatments such as denaturation and renaturation to restore activity. relatively difficult

The insect cell / baculovirus group expression system has the advantages of simplicity and high efficiency, and has the ability to post-translationally modify, process and transfer foreign proteins. When the source protein is expressed, the virus has already started apoptosis

Although the mammalian cell expression system has certain advantages in gene post-transcriptional modification, protein processing, secretion, glycosylation, etc., its expression cost is too high and it is not suitable for large-scale production

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