152 results about "Ascaris lumbricoides" patented technology

A composition, device, kit and method for detecting the presence or absence of roundworm in a fecal sample. The composition, device, kit and method of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm.

The invention discloses a compound bacterium agent and a method for accelerating fast maturity by inoculating compound bacterium agent to compost. The method comprises the steps of: performing enlargement cultivation respectively on high-temperature microbial strains such as Geobacillus, Bacillus licheniformis, two trichoderma, actinomyces and the like which are screened from the matured compost, and combining the strains in certain proportion into the compound bacterium agent. The compound bacterium agent is inoculated to the compost in certain proportion, the compost is moved into a fermentation tank for aerated aerobic fermentation, and the compost maturity is completed. Compared with the control compost, the inoculated compost of the scheme of the invention reaches the high temperature period one week earlier than the control compost, and the high temperature period lasts for 14 days so as to be beneficial to the decomposition and humification of organic matters of the compost; and the inoculated compost is thoroughly decomposed about twenty days later. Through test, most of the weed seeds, ascarid eggs and colibacillus in the compost are dead, the humic acid content is 25 g / kg higher than that of the control compost, the humification coefficient is more than 1.9, the decomposed finished product is black brown and odourless, and has excellent stability, harmless and minimized quality, apparently environmental and economic benefits.

Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and / or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

Methods, devices, kits and compositions for detecting the presence or absence of one or more helminthic coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm, whipworm and / or hookworm in a fecal sample from a mammal and may also be able to distinguish between one or more helminth infections. Confirmation of the presence or absence of roundworm, whipworm and / or hookworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and / or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

Methods, devices, kits and compositions for detecting the presence or absence of hookworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of hookworm in a fecal sample from a mammal that may also be infected with one or more of roundworm, whipworm, and heartworm. Confirmation of the presence or absence of hookworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and / or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

The invention relates to the field of veterinary drugs, and particularly relates to prevention and cure of three main parasitic diseases, namely a tapeworm disease, a roundworm disease and a coccidium disease, or the tapeworm and coccidium mixed infection and the roundworm and coccidium mixed infection of free-ranging chickens. The invention particularly discloses a traditional Chinese medicine composite for resisting the parasites of the free-ranging chickens and a preparation method thereof. The traditional Chinese medicine composite is prepared into a traditional Chinese medicine formulation by taking rangooncreeper fruits, aloes, liquorice, hawthorns, fructus carpesii, medicated leaven, fructus hordei germinatus, azedarach, rhizoma atractylodis, codonopsis pilosula, betel nuts, radix dichroae, cyrtomium fortunei, radix astragali and pericarpium citri reticulatae as crude drugs. The traditional Chinese medicine disclosed by the invention is used for treating the three main parasitic diseases, namely the tapeworm disease, the roundworm disease and the coccidium disease, or the tapeworm and coccidium mixed infection and the roundworm and coccidium mixed infection of the free-ranging chickens, enhances the feed conversion rate, strengthens the organism resistance action and has the advantages of outstanding effect, safety in use, no residue, difficulty in drug resistance generation, low price, and the like.

The invention discloses a method for preparing bio-organic fertilizer by utilizing quick odorless fermentation of chicken manure. The bio-organic fertilizer is prepared from microbial liquid inoculum, auxiliaries and chicken manure by means of two-step fermentation, and no foul smell, ammonia gas or other pollution gases is generated in the whole process. The bio-organic fertilizer is grayish brown odorless particles without mechanical impurities. By detection, in the bio-organic fertilizer, the content of lactic acid bacteria is more than or equal to 10<8>CFU / g, the content of total viable bacteria is more than or equal to 10<9>CFU / g, the content of organic matters is 57.67 percent, the content of total nutrient is 7.8 percent, no alive roundworm egg or pathogenic bacterium is contained, and the content of escherichia coli is less than or equal to 10<2>CFU / g, which is in accordance with the specification in the national standard of bio-organic fertilizer (NY 884-2012).

Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a mammalian sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and / or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

The invention discloses a traditional Chinese medicine for treating a roundworm disease in a biliary tract. The traditional Chinese medicine is characterized by comprising the following herbs in part by weight: 50 parts of dark plum, 25 parts of combretum indicum, 25 parts of chinaberry seed, 15 parts of fructus aurantii, 15 parts of pepper, 15 parts of torreya seed, 25 parts of areca nut, 15 parts of costus root and 5 parts of liquorice. According to characteristics of the roundworm disease in the biliary tract, the traditional Chinese medicine is formulated by a scientific method; the traditional Chinese medicine not only can kill roundworms, but also has the effects of circulating Qi and stopping pains, strengthening the spleen and promoting digestion, soothing the liver and benefiting the gallbladder, restoring normal functions of the spleen and the stomach, and eliminating complications caused by the roundworms in the biliary tract; in four years, among 200 patients taking the traditional Chinese medicine, the effective rate is 100% and the cure rate is 100%; with 8 hours after the traditional Chinese medicine is taken, the traditional Chinese medicine can take effect; and after 2-3-day continuous use, the roundworm disease in the biliary tract can be cured.

The invention discloses an indirect ELISA (Enzyme Linked Immunosorbent Assay) method for detecting anti-roundworm antibody by a recombined roundworm ALAg protein antigen. In the method, RNA (Ribonucleic Acid) of a roundworm adult body or infective egg serves as a template; an ALAg gene is amplified by RT-PCR (Reverse Transcriptase-Polymerase chain Reaction); an ALAg expression carrier is built, and expression bacteria are transformed for inducible expression; recombined protein is purified; and the indirect ELISA method is built by taking the purified protein as a coated antigen. A stable and specific indirect ELISA method for an anti-roundworm body IgG is built, can be used for the clinical monitoring and diagnosis for roundworm infection and is suitable to basically and clinically widely popularize.

The invention discloses a Chinese herbal medicine feed additive for piglets and a preparation method of the additive. A formula of the Chinese herbal medicine feed additive comprises components in parts by weight as follows: 56-158 parts of an insect repelling component, 23-55 parts of a conditioning component, 8-12 parts of a nutrition addition component, 5-15 parts of a luring component, 0.5-0.7 part of a compound antioxidant and 0.1-0.16 part of a mould-proof agent, wherein the insect repelling component and the conditioning component comprises bulk drugs as follows: argy wormwood leaf, stemona root, coix seed, pumpkin seed, semen amaranthi tricolorls , spike aletris herb, pagodatree flower, short-leaf lady fern, bark of paper mulberry, prickly ash, prickly ash root, prickly ash leaf, green bristlegrass herb, codonopsis radix, hawthorn fruit, germinated barley, dried tangerine peel and licorice root. The feed additive can dry damp, strengthen spleens, dispel and kill roundworms, warm spleens and stomachs, dispel cold, invigorate spleen, promote digestion, improve the immunity of the piglets, prevent disease and increase weight, and the feed additive can prevent and treat the ascariasis, regulate intestines and stomachs of the piglets, enhance the nutrition intake and greatly increase the weight gain rate when being used for feeding the piglets.

The invention provides a method for preparing high-quality biological organic fertilizer through municipal sewage sludge. The method includes the steps of raw material preparing, fermented material preparing, sterilization, anaerobic fermentation, fermentation promoter injection, aerobic fermentation and finished product preparing. Anaerobic fermentation agents used in the anaerobic fermentation step contain methane thermophilic micrococcocci, lactobacillus planetarium, metallophilic thermoanaerobacter ethanolicus and white high-temperature actinomycetes. Aerobic fermentation agents used in the aerobic fermentation step contain trichoderma reese, lactobacillus delbruckii and vibrio proteolyticus. According to the prepared bio-organic fertilizer, the number of fecal coliform bacteria ranges from 23 to 26 per g, the death rate of ova of roundworm ranges from 99.2% to 99.8%, and the number of effective living bacteria ranges from 440 millions CPF / g to 52 millions CPF / g.

Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a mammalian sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and / or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

The invention relates to an intestinal diagnosis robot system for diagnosing intestines of animals or human and the motion control method thereof, which belong to the field of biomedicine engineering. The system comprises an intestinal diagnosis robot and a controller, wherein the diagnosis robot includes a motion driver, a plurality of simulating electrodes, a diagnostic chamber and a flexible fixed ring; the simulating electrodes are fixedly connected with the motion driver; the diagnostic chamber includes a motion control circuit and a miniature diagnosis unit; the motion control circuit is connected with the simulating electrodes through a flexible circuit wire; the diagnostic chamber is flexibly and fixedly connected with the motion driver; and the motion driver can be intestinal parasites or transgenic intestinal parasites, such as ascaris lumbricoides and transgenic ascaris lumbricoides. The motion control method of the intestinal diagnosis robot system mainly comprises simulating the motion driver by use of the simulating electrodes. Compared with prior art, the intestinal diagnosis robot system can enter the deeper portion of intestines in an initiative drive manner, and has higher energy efficiency and better biocompatibility.

The invention discloses a traditional Chinese medicine prescription for treating biliary tract ascariasis. The traditional Chinese medicine prescription consists of the following components in parts by weight: 8-10 parts of raw rhubarb, 8-10 parts of ceylon helminthostachys rhizome, 4-6 parts of polygonum cuspidatum, 8-10 parts of smoked plum, 4-5 parts of manchurian wild ginger, 4-6 parts of rhizoma picrorhizae, 3-5 parts of Chicken's gizzard-membrane, 3-5 parts of pepper, 3-5 parts of areca seed, 8-10 parts of roughhaired holly root, 3-5 parts of pericarpium granati, 3-5 parts of radix scutellariae, 3-5 parts of quisqualis indica, 2-4 parts of neem bark, 3-5 parts of trogopterus dung, 4-6 parts of golden cypress, 6-9 parts of fructus aurantii, 3-5 parts of moringa and 4-6 parts of costus root. The traditional Chinese medicine prescription has the effects of clearing and promoting gallbladder, killing roundworms and clearing the hollow viscera, has a special effect on treating the biliary tract ascariasis, has a cure rate of 100 percent, is low in cost and does not have toxic or side effect.

The invention relates to an antibacterial peptide gene and a preparation method thereof; the antibacterial peptide gene provided by the invention is ascaris suum antibacterial peptide Cecropin P2; and coded ascaris suum antibacterial peptide Cecropin P2 is firstly separated from mammals in vivo. The antibacterial peptide gene provided by the invention is a gene Cecropin P2' obtained by optimizing the ascaris suum antibacterial peptide Cecropin P2; a sequence of the antibacterial peptide gene is SEWNo1; in the sequence, a sixth codon is changed from original ctc into ctg in an optimization method; a ninth codon, a twelfth codon and an eighteenth codon are changed from original aaa into aag in an optimization method; by dint of above-mentioned optimization, a target gene can be expressed preferably in a pichia pastoris system.

The invention discloses a biological organic fertilizer and a production method thereof, and belongs to the field of fertilizers. The biological organic fertilizer contains the following components: more than or equal to 20 million active living bacteria per gram, more than or equal to 45% of organic matters, more than or equal to 5% of total nutrient, less than or equal to 30% of water, and less than or equal to 100 fecal coliform per gram, and has the pH of 5.5-7, wherein the death rate of roundworm ova is more than or equal to 90%, and the validity period is more than or equal to 12 months. The biological organic fertilizer has the effects of a microbial fertilizer and an organic fertilizer, and can change wastes into valuable and protect the environment; and the production method can effectively shorten the production period and reduce the production cost.

The invention relates to a compound preparation for expelling in-vivo and in-vitro parasites from dogs and cats and a preparation method thereof. The compound preparation mainly comprises milbemycin oxime and praziquantel, wherein the milbemycin oxime is a novel and specific 16-membered ring macrolide medicament for preventing and controlling the in-vivo and in-vitro parasites of the dogs and the cats, which can specially prevent and control heartworms and efficiently prevent and control in-vivo parasites such as hookworms, roundworms, whipworms, belly worms and the like and in-vitro parasites such as hair follicle mites, scabies, louses, fleas and the like; and the praziquantel is effective to nematodes, trematodes and tapeworms in animal bodies. The milbemycin oxime and the praziquantel expel the parasites complementarily. The two medicaments are combined, so that a parasite expelling range is expanded and one-step parasite expelling effect is improved. The invention also provides a method for preparing compound tablets. The tablets prepared by the method can be released rapidly and dispersed fully.

The present invention provides roundworm egg detecting real-time fluorescence PCR primer and probe and the detection method therewith. Roundworm egg causing contamination on kraut and other botanical product is hard to detected morphologically with microscope. Through designing superfamily roundworm primer and probe to separate out suspected parasitic egg from kraut, repeated freezing and thawing with liquid nitrogen and fluorescent PCR operation, the suspected parasitic egg from kraut may be identified in high accuracy. The present invention has sensitivity enough high for detecting single roundworm egg, high specificity, and no cross reaction with other parasite egg and plant nematode egg.

The invention discloses an application of a eupatorium adenophorum extract in exterminating pig roundworm ova, which belongs to the technical field of pig roundworm ova extermination. According to the application, by improving the extraction process, effective components with insecticidal activity in the eupatorium adenophorum are extracted, the extract is applied to fresh pig roundworm ovas, the hatchability of the pig roundworm ovas is counted by observing through a microscope, and the result shows that the eupatorium adenophorum extract has good extermination effects on pig roundworm ova.

The invention relates to the technical field of biomedicine, in particular to a Rangoon creeper fruit extract and an extraction method thereof. According to the extraction method, polysaccharide is extracted from Rangoon creeper fruit by a biological enzymolysis technology, and the Rangoon creeper fruit extract with a higher polysaccharide purity is obtained by ethanol reflux extraction, water extraction, suction filtration, centrifugation, DEAE (diethylaminoethyl) column separation and purification, vacuum concentration and spray drying. The purity of the Rangoon creeper fruit polysaccharideis 72.7-78.2%, and the molecular weight of the polysaccharide is 21800-38900D. Shown by drug effect tests, the Rangoon creeper fruit extract provided by the invention has an inhibiting effect on S180sarcoma, has an effect on prolonging the life of a mouse with transplanted liver cancer H22, and can be used for preparing a medicament and / or health product for inhibiting tumor; and simultaneously,the Rangoon creeper fruit extract improves the effect on treating roundworm abdominal pain, infantile malnutrition, stagnation of milk and food, abdominal distention and diarrhea, and can be used forpreparing a medicament and / or health product for treating roundworm abdominal pain, infantile malnutrition, stagnation of milk and food, abdominal distention and diarrhea.