The invention belongs to the technical field of
plant root-knot
nematode inoculation methods, and particularly provides a
plant root-knot
nematode potting three-layer sick soil
inoculation method. Themethod comprises the steps of 1,
disease-free soil collection and treatment; 2, sick soil collection and treatment; 3, sick soil expanding propagation cultivation; 4, expanded propagation sick soil pretreatment; and 5, three-layer sick soil
inoculation. The
plant root-knot
nematode potting three-layer sick soil inoculation method adopts a three-layer sick soil inoculation technology that
disease-free soil is inoculated on upper and lower
layers, and nematode sick soil is inoculated on a middle layer. Compared with an existing root-knot nematode egg and larvae inoculation technology, the technology approaches natural inoculation, the inoculation
survival rate is high, a nematode selecting link is omitted, and the
workload is greatly reduced. Compared with an existing root-knot nematode single sick soil inoculation technology, the technology has the advantages that the usage amount of the sick soil is reduced by 1 / 2-2 / 3, and the
workload and cost of the collection, transportation, expanding propagation cultivation and the like of the sick soil are greatly reduced; and newly transplanted seedlings can be protected from being infected by nematodes, not only can the success of the inoculation be ensured, but also too
early disease death of the seedlings can be avoided.