Stem nematode disease resistance identification method for sweet potato related wild species
A technology for identification of resistance and stem nematode, which is applied in the identification of resistance to stem nematode disease of wild relatives of crops and the identification of resistance to stem nematodes of wild relatives of sweet potato, which can solve the problem that the identification method of resistance is not applicable. Wild species and other problems, to achieve the effect of shortening the test period, simple operation process, and short test period
Inactive Publication Date: 2014-05-21
JIANGSU XUZHOU SWEET POTATO RES CENT
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Problems solved by technology
Different from sweet potato, not all wild sweet potato species can form tuberous roots, so the method for identification of line resistance by punching inoculation is not suitable for wild species, and there is no standard method for identification of stem nematode resistance in wild sweet potato species
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Embodiment 1
[0025] Plant the wild sweet potato species to be identified in flowerpots filled with 1 kg of sterilized soil, and plant 1 plant in each pot; inoculate 200 stem nematodes into each pot using the root dripping method, and dig out the underground stem part of the wild species after 40 days to treat stem nematodes. The damage caused by the disease was investigated to evaluate the resistance of wild sweet potato species to stem nematode disease;
[0026] (1) Cultivation of D. nematodes: Collect tuber tubers infected with D. nematodes in the field and bring them back to the laboratory to separate D. nematodes by the shallow dish method. After microscopic examination confirmed that D. sweet potato was D. sweet potato, it was sterilized with 1% streptomycin sulfate, rinsed with sterilized water three times, and D. Cultivate in a 25°C incubator for later use.
[0027] (2) Preparation of materials to be tested: Put 1 kg of sterilized soil into a circular plastic flower pot with a diam...
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The invention discloses a stem nematode disease resistance identification method for sweet potato related wild species, and belongs to disease resistance identification methods of related wild species of crops. The identification method comprises the steps that the sweet potato wild species to be identified are planted into flowerpots containing sterilized soil, and one seedling is planted in each flowerpot; two hundred stem nematode worms are inoculated to each wild seedling by the adoption of the root dripping method, and the seedlings are cultivated at 25 DEG C, 80 percent of humidity, and a photoperiod of 14 / 10(L / D); the seedlings are watered appropriately each day, and the humidity of the soil in the flowerpots is controlled to be within the range of 18 percent-25 percent; after the seedlings are cultivated for 40 days, the subterraneous stems of the wild species are dug out and are vertically split with dissecting scissors, the stem nematode disease damage areas in the stems are measured, disease indexes are divided according to the ratio of the damaged areas to the stems, and the resistance levels of the wild species are determined. The processing means of the identification method is scientific and effective, the operation process is simple, the experimental period is short, and pollution to the environment by the test materials can be avoided. Besides, test results can comprehensively embody the levels of resistance to the stem nematode disease of the sweet potato related wild species.
Description
technical field [0001] The invention relates to a method for identifying resistance to stem nematode disease of wild species of close relatives of crops, in particular to a method for identifying resistance of wild species of sweet potato to stem nematode disease, and belongs to the field of agricultural plant protection. Background technique [0002] my country is the largest sweet potato producer in the world. In recent years, the sweet potato planting area is about 70 million mu, accounting for about 50.0% of the world's sweet potato planting area. Ditylenchus destructor Thome (Ditylenchus destructor Thome) is the causative agent of Ditylenchus destructor Thome. Stem nematodes. Sweet potato stem nematode disease is mainly distributed in Hebei, Henan, Shandong, Shanxi, Anhui, Jiangsu and other provinces in China. The disease can cause rotten cellars during the storage period of sweet potatoes, and rotten beds during the seedling raising period. After the sweet potato is...
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IPC IPC(8): A01G1/00A01G7/00A01K67/033
Inventor 徐振谢逸萍赵永强曹清河孙厚俊唐君杨冬静张成玲魏猛
Owner JIANGSU XUZHOU SWEET POTATO RES CENT
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