43 results about "Ditylenchus destructor" patented technology

The invention discloses a nematicidal composition and application thereof. The composition comprises active ingredients A and B, and a mass ratio of A to B is 20:1 to 1:20. The ingredient A refers to one of fosthiazate, abamectin, furfural, dazomet, fluopyram, carbosulfan, metham, verticillium chlamydosporium, emamectin benzoate, paecilomyces lilacinus or fluensulfone; and the structural formula of the ingredient B is a formula I or a formula II, wherein the formula I is as shown in the specification, and the formula II is as shown in the specification. The nematicidal composition disclosed by the invention is used for killing plant parasite nematodes comprising root-knot nematodes, ditylenchus destructor, heterodera sachachtii, pratylenchus coffeae and bursaphelenchus xylophilus, is composed of a novel nematicide containing lactonic rings and the existing insecticides and bactericides, and has good nematocidal activity and low toxicity to people and livestock. The nematicidal composition disclosed by the invention has an excellent effect of controlling eggs and second-stage larvae of the root-knot nematodes.

The invention discloses a kit for detecting Ditylenchus destructor based on loop-mediated isothermal amplification and application thereof. The invention provides a special primer for assistant identification of Ditylenchus destructor and consists of DNAs shown in sequences 1-14 in a sequence table. The kit provided by the invention comprises the special primer. According to the invention, the kit for assistant identification of Ditylenchus destructor is provided through designing the specific primer and optimizing reaction conditions; and through applying the kit, an interspecies level and an infraspecies level of detection can be achieved, and the problems that the routine molecule detection method has the defects of high cost, low efficiency and slow speed and can not meet the requirements of current plant quarantine and plant protection are solved.

The invention discloses an RPA primer group for identifying Ditylenchus destructor. The RPA primer group comprises primers and a probe, wherein the primers consist of nucleotide sequences shown as SEQID No. 1 and SEQ ID No. 2, and the probe consists of nucleotide sequences shown as SEQ ID No. 3. The invention also discloses a detection kit containing the primer group and a constant-temperature fluorescence amplification detection method for identifying the Ditylenchus destructor by utilizing the primer group. The RPA primer group and the detection method thereof provided by the invention havethe advantages of strong specificity, high sensitivity, high detection speed, low reaction temperature, simple operation and low cost when being used for detecting the Ditylenchus destructor, and canmeet the requirements of port quarantine and field real-time detection.

The invention discloses a plant-source nematicide for preventing and curing ditylenchus destructor of angelica sinensis, and application of the plant-source nematicide. The nematicide is prepared form 2-15% of stellera chamaejasme extrct and 85-98% of kieselguhr in percentage by weight. The plant-source nematicide has a remarkable inhibiting effect on the ditylenchus destructor of angelica sinensis, in addition, the yield of the angelica sinensis is remarkably increased, the condition, the quality and the medicinal value of the angelica sinensis are improved, the nematicide is environmentally-friendly, does not have any residue, is popularized and applied in the plant production mode of the angelica sinensis, and has excellent economic and ecological benefits.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer group and method for rapidly detecting ditylenchus destructor from complex samples. The LAMP primer group comprises a pair of outer primers F3/B3, a pair of inner primers FIP/BIP and a loop primer LF, and sequences of the primers are sequentially shown as SEQ ID NO. 1-5. The LAMP primer group and the detection method thereof disclosed by the invention have excellent specificity and sensitivity for detecting the ditylenchus destructor, individuals of the ditylenchus destructor in different insect stages (such as eggs, larva, female and male) can be detected, the ditylenchus destructor can be directly detected from multiple nematode mixed samples, plant tissue samples and soil samples, and the detection sensitivity can reach 1/1000 individual DNA. A novel detection method is provided for rapid detection and identification and early diagnosis of the ditylenchus destructor, and the method has the advantages ofbeing accurate, sensitive, stable and intuitive in result judgment. The LAMP primer group disclosed by the invention is simple and convenient to operate and high in practicality, and has important significances and application value on quarantine on ports and transfer and origin monitoring operations.

The invention provides a recombinase polymerase amplification (RPA) primer for detecting ditylenchus destructor thorne by the RPA technology and an RPA kit comprising the RPA primer, and the RPA primer has sequences shown in SEQ ID No: 5 and SEQ ID No: 6. The invention also provides a method for detecting the ditylenchus destructor thorne by using the RPA kit. The method can detect single or mixedditylenchus destructor thorne DNA samples, is accurate in detection, high in sensitivity, simple to operate, short in time and good in repeatability, and provides theoretical guidance for diagnosis detection and scientific pesticide application of the ditylenchus destructor thorne.

The invention discloses a method for purification culture of sweet potato rot stem nematodes, which comprises the following steps of: (1) picking older young female and male nematodes in the rot stem nematode by a needle and placing on a container standby; and (2) transversely or vertically cutting the whole potato, perforating holes on one cross section, injecting little sterile water into each hole in a standard that the water does not overflow from the holes, then, inoculating the rot stem nematode, one older young female and two males, obtained in the first step into the small holes, then, covering the cut potato blocks onto the potato blocks inoculated with the rot stem nematode as a natural moisture preservation cover, sealing the edge gaps by paraffin wax, placing the potato blocks into a culture box after being bound by elastic materials, carrying out culture for 30 days at 25 DEG C, and obtaining a plurality of pure rot stem nematodes. The method provided by the invention is simple, the operation is easy, the workload is low, more importantly, the purity of the obtained group is high, the vitality is good, and the assurance is provided for the systematic study work on the biological characteristics, the pathogenic mechanisms, the population groups, the individual genetic variation and the like of the nematode.

The invention discloses a medicinal fertilizer with concentrated biogas slurry as a carrier coupled with allothigene to prevent and treat sweet potato ditylenchus destructors. The medicinal fertilizer comprises the following components in parts by weight: one or two allothigenes, 0.1-3% of organic silicon, 3-10% of aids and the balance of concentrated biogas slurry, wherein each allothigene accounts for 0.1-10%. As the biogas slurry is coupled with the allothigenes, the medicinal fertilizer has remarkable synergic effects in preventing and treating the sweet potato ditylenchus destructors, is capable of greatly shortening the lethal time of nematode, meanwhile is capable of improving soil structures and fertility situations of agricultural fields, is capable of increasing single yield of crops, and has dual effects of killing insects and improving fertility.

The invention relates to the technical field of Chinese herbal medicine disease control and pesticides and specifically relates to a control method for angelica sinensis ditylenchus destructor thorne. The method comprises the steps of (1) cultivation, (2) soil treatment, (3) seedling soaking, and (4) pesticide control. The method employs low-toxicity and low-residue pesticide control, and thus reduces the residue quantity of pesticides, and guarantees production of safe angelica sinensis; control is performed in the soil treatment, seedling soaking and growing periods and different kinds of pesticides are applied in the different phases, so that drug resistance is prevented; the input cost is reduced and the yield is increased.

The invention discloses an angelica sinensis-garlic intercrop-planting method. The angelica sinensis-garlic intercrop-planting method comprises the steps that holes are dug in land to be farmed; angelica sinensis seeds are planted in the holes; garlic is planted among angelica sinensis rows in the same period in a predetermined intercropping mode, wherein an angelica sinensis planting mode is seedling culture and transplantation, and a garlic planting mode is bulb transplantation. By adopting the method, successive cropping obstacles of the angelica sinensis can be effectively relieved, the usage efficiency of per unit farmed land is improved, the yield risk is reduced, the angelica sinensis ferulic acid and volatile oil content can be obviously improved under continuous cropping and crop rotation conditions compared with transitional single culture, the angelica sinensis quality can be improved, increase of soil fungi can be inhibited under the effect of intercropped garlic, the bacterium and actinomycete decreasing speed can be reduced, soil enzyme activity can be improved, and the soil nutrient utilization rate of the angelica sinensis can be improved. The method is conductive to stability and improvement of a soil environment, a central inhibiting effect is played on soil diseases, the incidence of ditylenchus destructor of the angelica sinensis is remarkably reduced, the fertilizer application amount can be decreased, and the improvement of the angelica sinensis quality can be obviously promoted.

The invention relates to pyrethroid pesticide and a composition for preventing the plant nematodiasis. The active component for killing the nematode is deltamethrin, composition of deltamethrin and abamectin or composition of deltamethrin and carbofuran. The deltamethrin has a good preventing effect for the plant nematode such as the ditylenchus destructor throne, heterodera elachista, meloidogyne incognita chitwood and rice root-knot nematode and can prevent the infection of the nematode on the host plant, after compounding the deltamethrin and the abamectin and compounding the deltamethrin and the carbofuran, the synergism is obvious, and the control efficacy can be improved.

The invention discloses a traditional Chinese medicine extract having the Ditylenchus destructor Thorne resisting activity and application thereof. Fresh Chinese angelica stems and leaves are taken and naturally dried or are dried at the low temperature of 60 DEG C and then are smashed and screened to obtain dried Chinese angelica stems and leaves, the Chinese angelica stems and leaves are taken to perform extraction by using petroleum ether, ethyl acetate, n-butanol or water the obtain an extract, and the extract is concentrated and dried to obtain the traditional Chinese medicine extract having the Ditylenchus destructor Thorne resisting activity. The traditional Chinese medicine extract uses the waste Chinese angelica stems and leaves as a raw material, the biological activity of the traditional Chinese medicine extract is screened through a large number of experiments, and the traditional Chinese medicine extract having very good Ditylenchus destructor Thorne resisting activity is obtained through optimization. The waste Chinese angelica stems and leaves are fully utilized and are turned into wealth, the prepared extract can be used for preparing green environmentally-friendly high-efficiency low-toxicity biological pesticides, has important environmental protection significance and economic value and is worthy of popularization and application.

The invention relates to the technical field of microorganisms, in particular to a composite microbial agent of Clonostachysrosea and Massiliatimonae and application in disease control. The Clonostachysrosea is Clonostachysrosea fhnzm, the preservation number is CGMCC No.17070, and the Massiliatimonae is Massiliatimonae BMMSJS-1, and the preservation number is CGMCC No.18668. The composite microbial microbial agent bacterial solution can fix nitrogen and promote the growth of seedlings, and can preferentially symbiose with the seedlings in a sterile soilless substrate seedling culture mode tobreed high-quality seedlings carrying probiotics. The composite microbial microbial agent bacterial liquid has better effects on growth promotion of alpine shade plants and prevention of plant diseases and insect pests than probiotic bacteria. The composite microbial microbial agent bacterial liquid can be used for preventing and treating root rot, ditylenchus destructor and the like of alpine shade plants and promoting growth of alpine nitrogen-loving plants.

The invention belongs to the technical field of biological pesticides and relates to application of trans-aconitic acid in controlling plant pathogenic nematodes and harmful insects. According to bioassay, the trans-aconitic acid has the activity of obviously killing meloidogyne incognita chitwood, heterodera glycine ichinohe and ditylenchus destructor, and the activity of suppressing the growth of cotton bollworms and houseflies; the concentration of the trans-aconitic acid used for the meloidogyne incognita chitwood is 100-1000 [mu]g/mL, preferably 500 [mu]g/mL, with the death rate of 85.5 percent; 200-1000 [mu]g/mL for the heterodera glycine ichinohe, preferably 500 [mu]g/mL, with the death rate of 99.2 percent; 500 [mu]g/mL for the ditylenchus destructor with the death rate of 53.4 percent; 10-1000 [mu]g/mL for the cotton bollworms, preferably 200 [mu]g/mL, with the growth suppressing rate of 34.0 percent; and 10-1000 [mu]g/mL for the houseflies, preferably 200 [mu]g/mL, with the growth suppressing rate of 13.4 percent. The trans-aconitic acid is expected to become a pesticide composition for controlling plant pathogenic nematodes, cotton bollworms and houseflies.

The invention discloses a method for screening an anti-plant nematode infection compound by taking 2-Cys Prx type peroxiredoxin as a target. The method comprises the following steps: constructing recombinant expression plasmids pET41b-DdPrx containing Ditylenchus destructor 2-Cys Prx genes; transferring the recombinant expression plasmids into BL21 (DE3) competent cells, inducing the culture by virtue of IPTG so as to stably express recombinant Dd-PRX proteins; and co-incubating a candidate compound and the recombinant DdPrx proteins, and detecting whether the candidate compound has in-vitro inhibitory activity on the recombinant DdPrx proteins according to a ferric thiocyanate method. The method has the advantages of directness, simplicity, convenience and rapidness, can be used for manual screening and also can be used for screening large-scale high flux inhibitors. The method can be used for researching, developing and screening medicines for resisting plant nematode infection.