Method for screening nematicide by utilizing pluronic F127 and sweet potato stems

A technology of F127 and Pluronic, which is applied in the field of pesticide screening, can solve problems such as missed screening, and achieve the effects of accurate results, high screening accuracy, and easy-to-obtain materials
CN104483447AInactive Publication Date: 2015-04-01INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
Publication Date
2015-04-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for screening nematicide by utilizing pluronic F127 and sweet potato stems, belonging to the field of a pesticide screening method. The method comprises the following steps: by taking 20-40% by mass of pluronic F127 solution as a matrix, adding a to-be-tested medicament and nematode solution into the matrix, standing for 48-72h at room temperature, then inserting sweet potato stems, culturing for 3-15d, taking out sweet potato stems, counting nematodes intruded into the sweet potato stems and calculating the control effect. Compared with an impregnation method, the method can be used for comprehensively evaluating the lethal effect of the to-be-tested medicament to nematodes, as well as the inhibition action to the action capability and intrusion capability of the nematodes, the screening accuracy is high, and the reliability is high; secondly, the sweet potato stems and sweet potato stem ditylenchus destructors are easily propagated indoors, and the materials are easily available and suitable for screening of massive medicaments; in addition, plant-parasitic nematodes are taken as test objects in the method, and compared with the common caenorhabditis elegans, the detection effect is more accurate and reliable.
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Description

technical field

[0001] The invention belongs to the field of pesticide screening methods, in particular to a method for screening nematicides by using Pluronic F127 and sweet potato stems. Background technique

[0002] Plant nematodes are one of the main pathogens in agricultural production, causing economic losses of about 100 billion US dollars to the world's agricultural production every year. The control methods of plant nematodes include disease-resistant varieties, crop rotation, flooding, sun exposure, biological control, etc. (Roland N.P. et al. Plant Nematology, CABI Publishing.2013.), but chemical control is still a fast, cost-effective control method, which is well received Favored by farmers.

[0003] Traditional nematicide screening mostly takes Caenorhabditis elegans as the test object to evaluate the toxicity of the test agent to the nematode (Ohba K et al. Journal of Pesticide Science, 1984, 9:91-96). At present, commercial nematicides are all obtained thro...

Claims

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