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Method for screening nematicide by utilizing pluronic F127 and sweet potato stems

A technology of F127 and Pluronic, which is applied in the field of pesticide screening, can solve problems such as missed screening, and achieve the effects of accurate results, high screening accuracy, and easy-to-obtain materials

Inactive Publication Date: 2015-04-01
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem that the existing method for screening nematicides is easy to cause leakage, the purpose of the present invention is to provide a method for screening nematicides using Pluronic F127 and sweet potato stems

Method used

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  • Method for screening nematicide by utilizing pluronic F127 and sweet potato stems
  • Method for screening nematicide by utilizing pluronic F127 and sweet potato stems
  • Method for screening nematicide by utilizing pluronic F127 and sweet potato stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Utilize Pluronic F127 and Sweet Potato Stem Screening Nematicide Comparison Test

[0025] (1), test design: select conventional nematocides 80% aldicarb (Shandong Huayang Pesticide Chemical Group Co., Ltd.), 95% abamectin (Hebei Weiyuan Biochemical Pesticide Co., Ltd.), 90% clofenac Granville (Jiangsu Jialong Chemical Co., Ltd.) was used as the test chemical agent, and the treatment without adding the agent was used as the control.

[0026] (2) Test method:

[0027] (1) Put 35g of Pluronic F127 into 100ml of distilled water and dissolve overnight at a low temperature of 4°C to obtain the mother liquor of Pluronic F127.

[0028] (2) Ditylenchus destructor (Ditylenchus destructor) was prepared into a liquid of 100 heads / mL with distilled water to obtain a nematode liquid.

[0029] (3), 3 kinds of medicaments of 80% aldicarb, 95% abamectin, and 90% carbosulfur original drug were dissolved with acetone respectively, and then diluted with water to form a mother l...

Embodiment 2

[0037] Embodiment 2 The present invention utilizes Pluronic F127 and sweet potato stem screening nematocide comparison test

[0038] (1) Drugs to be tested: the conventional nematicides aldicarb, abamectin, and carbosulfan were selected as the tested drugs, and the effects of different detection times on drug efficacy evaluation were compared.

[0039] (2), the specific operation steps are the same as in Example 1, and the control effect of the medicament is checked after 5d, 6d, 7d, and 8d of contact between the medicament and the nematode respectively. That is, insert the sweet potato stem section of Jishu 98 (susceptible variety) in the method of the present invention for 3d, 4d, 5d, and 6d, and then check after 5d, 6d, 7d, and 8d in the dipping method. The test concentrations of carboxycarbamethylene, abamectin and carbosulfan were all 200ug / mL. The treatment without drug was used as the control.

[0040] Result (see table 2) at all test times, the preventive effect that...

Embodiment 3

[0043] Embodiment 3 Utilize Pluronic F127 and the sweet potato stem of different varieties to screen nematocide comparative test

[0044] (1) Experimental design: the conventional nematicides aldicarb, abamectin and carbosulfan were selected as the test agents, and the treatment without adding the agents was used as the control treatment. The effects of different sweet potato varieties on the efficacy evaluation were compared.

[0045] (2) test method: the specific operation steps are the same as embodiment 1, select respectively the sweet potato stems of Jishu 98, No. 4 Jishu, Xushu 18, and Xushu 25 as the host, and check the results in the 5th day; The test concentrations of carboxycarbamethylene, abamectin and carbosulfan were all 150ug / mL, and the treatment without addition of medicine was used as the control.

[0046] Result (seeing table 3) selects different sweet potato varieties for the evaluation result trend of drug effect to be the same, the nematode control effect...

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Abstract

The invention discloses a method for screening nematicide by utilizing pluronic F127 and sweet potato stems, belonging to the field of a pesticide screening method. The method comprises the following steps: by taking 20-40% by mass of pluronic F127 solution as a matrix, adding a to-be-tested medicament and nematode solution into the matrix, standing for 48-72h at room temperature, then inserting sweet potato stems, culturing for 3-15d, taking out sweet potato stems, counting nematodes intruded into the sweet potato stems and calculating the control effect. Compared with an impregnation method, the method can be used for comprehensively evaluating the lethal effect of the to-be-tested medicament to nematodes, as well as the inhibition action to the action capability and intrusion capability of the nematodes, the screening accuracy is high, and the reliability is high; secondly, the sweet potato stems and sweet potato stem ditylenchus destructors are easily propagated indoors, and the materials are easily available and suitable for screening of massive medicaments; in addition, plant-parasitic nematodes are taken as test objects in the method, and compared with the common caenorhabditis elegans, the detection effect is more accurate and reliable.

Description

technical field [0001] The invention belongs to the field of pesticide screening methods, in particular to a method for screening nematicides by using Pluronic F127 and sweet potato stems. Background technique [0002] Plant nematodes are one of the main pathogens in agricultural production, causing economic losses of about 100 billion US dollars to the world's agricultural production every year. The control methods of plant nematodes include disease-resistant varieties, crop rotation, flooding, sun exposure, biological control, etc. (Roland N.P. et al. Plant Nematology, CABI Publishing.2013.), but chemical control is still a fast, cost-effective control method, which is well received Favored by farmers. [0003] Traditional nematicide screening mostly takes Caenorhabditis elegans as the test object to evaluate the toxicity of the test agent to the nematode (Ohba K et al. Journal of Pesticide Science, 1984, 9:91-96). At present, commercial nematicides are all obtained thro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 王容燕陈书龙李秀花高波马娟
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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