31 results about "Soil fungi" patented technology

A biological soil nutrient system that combines beneficial soil fungi and bacteria in a growth promoting nutrient medium, embedded in an inorganic porous ceramic particle for direct delivery during soil aerification to the rhizosphere of adventitious plants, including sports turf, landscape and agricultural applications.

The invention relates to a bacterial strain and a bacterial agent thereof for preventing and treating fungal diseases of peanut soil in continuous cropping. The bacterial strain is a kind of Pseudomonas protegens, and the preservation number is CGMCC No.11539. The 16s rRNA base sequence of the strain is shown in SEQ ID No:1. The strain of the present invention produces chitinase (degrades mycelium cell wall of pathogenic fungus) with high activity, and has remarkable degrading ability to mycelium of various soil-borne fungal pathogens such as Fusarium, Rhizoctonia, Verticillium and the like. The seed dressing dosage of the bacterial agent of the present invention is generally only 20-30 kg / mu, which can effectively prevent and control frequent soil-borne fungal diseases such as peanut root rot, blight, and silkworm in dry land, with a control effect of more than 55%. It has the advantages of small dosage, wide antibacterial spectrum, and significant yield-increasing effect, and can effectively reduce the impact of soil-borne diseases on the yield loss of continuous cropping ground peanuts.

The invention relates to a method for degrading residual tylosin in herb residues, in particular to a method for degrading residual tylosin in herb residues by utilizing microorganism, which mainly comprises the steps of preparation of soil fungi suspension, preparation of strain breeding cultural medium, preparation of strain domestication cultural medium, preparation of strain screening cultural medium, preparation of stain activation and rejuvenation cultural medium, preparation of drug residues fermentation cultural medium, screening of degrading strain and so on. The invention provides acompound bacterium that can be screened and cultured to degrade the residual tylosin in herb residues, while strains for degrading the residual tylosin in herb residues can be acquired by being separated and purified from the compound bacterium. Then the strains can be prepared to be corresponding herb residue cultural medium, which can degrade the residual tylosin in herb residues after being accessed the fermentation of the degrading bacteria.

The invention discloses an efficient cultivation method for improving soil acidity, precise fertilization and matched intercropping to comprehensively prevent and control banana wilt. Soil pH is regulated through a soil testing formula, precise fertilization is performed according to soil fertility, main banana nutrition NPK absorption amount, the rate of absorption and utilization and target yield, meanwhile peanut intercropping is performed to conserve water and soil, nodule nitrogen fixation is beneficial to banana growth, the soil microbial diversity is improved, and the occurrence rate of the banana wilt is remarkably reduced. The cultivation method is simple and clear to operate, the soil pH and the diversity of organic matter, bacteria and actinomycetes are remarkably improved, the amount of soil fungi (phytopathogens) is decreased, and the banana wilt is decreased. In addition, the fertilizer usage amount is effectively decreased, the high peanut and banana yield is obtained. The efficient cultivation method has the positive significance on prevention and control of the banana wilt and chemical fertilizer decrease and replacement through land applying and maintaining biological measures and efficient cultivation mode and has the application values on remarkable improvement of banana productivity, economic benefits, environment protection of producing areas and sustainable development of banana production.

The invention specifically discloses application of Agromyces sp. MT-E in degradation of a variety of phthalates, which belongs to the technical field of biological treatment of environmental pollutants. The Agromyces sp. MT-E is preserved in China Center for Type Culture Collection (CCTCC) on January 22, 2015, with an accession number of CCTCC M2015054. The Agromyces sp. MT-E can use DBP and DEHP as sole carbon source for aerobic degradation, and degradation rates reach 97.06% and 86.25% respectively when the Agromyces sp. MT-E is cultured in inorganic salt mediums respectively including DBP and DEHP with a concentration of 200 mg / L as the sole carbon source for 7 d. After the Agromyces sp. MT-E is inoculated into contaminated soil for 10 d, the degradation rates of DBP and DEHP in the soil reach 77.29% and 55.51%, respectively; and such high-efficiency degradation rates prove that the Agromyces sp. MT-E has good application prospect in restoration of soil contaminated by PAEs.

The invention discloses a method for transforming podophyllotoxin into 4-(2,3,5,6-tetramethylpyrazine-1-group)-4'-demethylepipodophyllotoxin, comprising the following steps of: (1) adding podophyllotoxin into a liquid fermentation medium, and carrying out biotransformation by inoculating second-class liquid seeds of humic soil fungi; (2) separating and purifying the product from the Step (1), followed by adding into the liquid fermentation medium, carrying out biotransformation by inoculating second-class liquid seeds of podophyllum taxa endophytes; then continuously carrying out biotransformation by adding ligustrazine, and collecting the transformation product. The invention also discloses a method for purifying 4-(2,3,5,6-tetramethylpyrazine-1-group)-4'-demethylepipodophyllotoxin from the above product and a method for detecting the content of 4-(2,3,5,6-tetramethylpyrazine-1-group)-4'-demethylepipodophyllotoxin. According to the invention, the molecular structure of podophyllotoxin is modified by the biotransformation method, which has advantages of mild reaction condition, low E-factor, quality controllability and simple separation process.

The invention relates to a method for protecting plants from pests, in particular bacteria, fungi, and viruses, using a pesticide composition with adjustable persistence and degradability, having thefollowing steps: providing a pesticide composition which comprises at least one poly(alkyl)guanidine with a monomer alkyl chain length ranging from C2 to C20 and a degree of polymerization n ranging from 2 to 600, and applying the pesticide composition to plant cultures for a specified time and in sufficient quantities in order to prevent or inhibit an adversive effect of the pests on the plants,wherein the length of the monomer alkyl chain and / or the degree of polymerization are set depending on at least one characterizing parameter of the soil or nutrient medium in which the plants are cultivated such that a specified or desired persistence and degradability of the pesticide composition on the cultures or in the soil or nutrient medium are obtained. In a special embodiment of the methodaccording to the invention, the at least one characterizing parameter of the soil or nutrient medium is determined in a first step, and the parameter is preferably selected from the pH value, the humus content, the humin content, the content of microorganisms, the content of soil fungi, or the content of soil bacteria.