Method for preparing biocontrol bacterial fertilizer from bacteria and method for eliminating continuous cropping obstacles of muskmelons

A technology of bio-control bacteria and bacteria, which is applied in the field of preparing bio-control bacterial fertilizer by bacteria and eliminating the obstacles of continuous cropping of melons. It can solve problems such as inability to eliminate self-toxic substances, unfavorable crop growth, and deterioration of soil physical and chemical properties, and achieve stable bacterial fertilizer products. Safe, promote the growth of soil bacteria, improve the effect of physical and chemical properties

Inactive Publication Date: 2021-09-03
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Soil-borne diseases eliminate soil fungi through root irrigation and other measures, but when pesticides are used to kill soil fungi, they also kill other microorganisms in the soil; long-term production of a single crop variety will eventually lead to deterioration of soil physical and chemical properties, It is not conducive to the growth of crops; and the application of agricultural and chemical fertilizers can promote the growth of crops, but it cannot eliminate the self-toxic substances produced by plant roots in the soil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The present invention provides a kind of method that bacterium prepares biocontrol bacterial fertilizer, comprises the following steps specifically:

[0026] a. using the Bacillus subtilis isolated in the vineyard as a bacterial strain to carry out the isolation and identification of the bacterial species;

[0027] b. Rice bran is sterilized by moist heat at 80Kpa and 100°C;

[0028] c. adding sterilized 1% soybean powder, 2% ammonium nitrate, 0.2% sucrose, 0.1% magnesium sulfate, 0.02% calcium sulfate, and 0.02% manganese sulfate to the carrier matrix of step b, And add the sterile water of carrier matrix weight 50% and the seed liquid of biocontrol bacteria of carrier matrix weight 1%, the seed liquid of biocontrol bacteria is the fresh bacterial liquid that storage time is no more than 36-48h, fully stirs evenly, obtains fermentation raw materials;

[0029] d. Ferment the fermented raw material obtained in step c in a heat preservation device, set the initial tempe...

Embodiment 2

[0035] The present invention provides a kind of method that bacterium prepares biocontrol bacterial fertilizer, comprises the following steps specifically:

[0036] a. using the Bacillus subtilis isolated in the vineyard as a bacterial strain to carry out the isolation and identification of the bacterial species;

[0037] b. Wheat husks are sterilized by moist heat at 90Kpa and 110°C;

[0038] c. adding sterilized 2% soybean powder, 3% ammonium nitrate, 0.3% sucrose, 0.3% magnesium sulfate, 0.04% calcium sulfate, and 0.03% manganese sulfate to the carrier matrix of step b, And add the sterile water of carrier matrix weight 55% and the seed solution of biocontrol bacteria of carrier matrix weight 1.1%, the seed solution of biocontrol bacteria is the fresh bacterial solution that storage time is no more than 36-48h, fully stirs evenly, obtains fermentation raw materials;

[0039] d. Ferment the fermented raw material obtained in step c in a heat preservation device, set the in...

Embodiment 3

[0045] The present invention provides a kind of method that bacterium prepares biocontrol bacterial fertilizer, comprises the following steps specifically:

[0046] a. using the Bacillus subtilis isolated in the vineyard as a bacterial strain to carry out the isolation and identification of the bacterial species;

[0047] b. The corn material is sterilized by moist heat at 100Kpa and 130°C;

[0048] c. adding sterilized 3% soybean powder, 4% ammonium nitrate, 0.5% sucrose, 0.1% magnesium sulfate, 0.06% calcium sulfate, and 0.05% manganese sulfate to the carrier matrix of step b, And add the sterile water of carrier matrix weight 65% and the seed liquid of biocontrol bacteria of carrier matrix weight 1.2%, the seed liquid of biocontrol bacteria is the fresh bacteria liquid that storage time is no more than 36-48h, fully stirs evenly, obtains fermentation raw materials;

[0049] d. Ferment the fermented raw material obtained in step c in a thermal insulation device, set the in...

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Abstract

The invention discloses a method for preparing a biocontrol bacterial fertilizer from bacteria and a method for eliminating continuous cropping obstacles of muskmelons, and the method comprises the following steps: by taking bacillus subtilis separated in a vineyard as a strain, separating and identifying the strain; sterilizing the carrier substrate; and adding sterilized soybean meal, ammonium nitrate, cane sugar, magnesium sulfate, calcium sulfate and manganese sulfate. The biocontrol bacteria can effectively inhibit the growth of soil fungi and promote the growth of soil bacteria through biocontrol bacteria, the biocontrol bacteria fertilizer produced from rice bran, wheat bran or crushed corn particles is applied to melon continuous cropping soil, the biocontrol bacteria can be brought in, a large amount of organic matter can be brought into the soil, the raw materials are easy to obtain, the price is low, and the operation is easy; lime nitrogen and quicklime are applied to soil, most pathogenic bacteria in the soil can be killed, soil-borne diseases are effectively reduced, the amino acid foliar fertilizer is sprayed, crop growth is promoted, crop quality is improved, and the quality and yield of muskmelons can be improved by spraying the amino acid foliar fertilizer in the growth period of the muskmelons.

Description

technical field [0001] The invention relates to the technical field of planting, in particular to a method for preparing biocontrol bacterial fertilizer by bacteria and a method for eliminating obstacles to continuous cropping of melons. Background technique [0002] Continuous cropping obstacle refers to the phenomenon that after planting the same species or the same family of crops in the same plot for many years, even if normal cultivation and management measures are adopted, the plant growth will be weakened, the pests and diseases will be aggravated, and the yield and quality will decline. In ancient my country, there were some records about the aggravation of crop diseases after continuous cropping. It was called continuous stubble disease, and European and American countries called it soil-borne disease, continuous cropping obstacle, and replanting disease. Continuous cropping obstacles are manifested as: weak growth of seedlings, poor root development, susceptibility...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/60C05G3/80A01C21/00A01G13/00A01G22/05
CPCC05G3/00C05G3/60C05G3/80C05C1/00A01G22/05A01G13/00A01C21/005C05D3/00C05D5/00C05D9/02C05F11/00C05F11/08C05F17/20C05F17/70
Inventor 农倩廖仕同龙艳艳李长宁
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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