Method for preparing biocontrol bacterial fertilizer from bacteria and method for eliminating continuous cropping obstacles of muskmelons
A technology of bio-control bacteria and bacteria, which is applied in the field of preparing bio-control bacterial fertilizer by bacteria and eliminating the obstacles of continuous cropping of melons. It can solve problems such as inability to eliminate self-toxic substances, unfavorable crop growth, and deterioration of soil physical and chemical properties, and achieve stable bacterial fertilizer products. Safe, promote the growth of soil bacteria, improve the effect of physical and chemical properties
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Embodiment 1
[0025] The present invention provides a kind of method that bacterium prepares biocontrol bacterial fertilizer, comprises the following steps specifically:
[0026] a. using the Bacillus subtilis isolated in the vineyard as a bacterial strain to carry out the isolation and identification of the bacterial species;
[0027] b. Rice bran is sterilized by moist heat at 80Kpa and 100°C;
[0028] c. adding sterilized 1% soybean powder, 2% ammonium nitrate, 0.2% sucrose, 0.1% magnesium sulfate, 0.02% calcium sulfate, and 0.02% manganese sulfate to the carrier matrix of step b, And add the sterile water of carrier matrix weight 50% and the seed liquid of biocontrol bacteria of carrier matrix weight 1%, the seed liquid of biocontrol bacteria is the fresh bacterial liquid that storage time is no more than 36-48h, fully stirs evenly, obtains fermentation raw materials;
[0029] d. Ferment the fermented raw material obtained in step c in a heat preservation device, set the initial tempe...
Embodiment 2
[0035] The present invention provides a kind of method that bacterium prepares biocontrol bacterial fertilizer, comprises the following steps specifically:
[0036] a. using the Bacillus subtilis isolated in the vineyard as a bacterial strain to carry out the isolation and identification of the bacterial species;
[0037] b. Wheat husks are sterilized by moist heat at 90Kpa and 110°C;
[0038] c. adding sterilized 2% soybean powder, 3% ammonium nitrate, 0.3% sucrose, 0.3% magnesium sulfate, 0.04% calcium sulfate, and 0.03% manganese sulfate to the carrier matrix of step b, And add the sterile water of carrier matrix weight 55% and the seed solution of biocontrol bacteria of carrier matrix weight 1.1%, the seed solution of biocontrol bacteria is the fresh bacterial solution that storage time is no more than 36-48h, fully stirs evenly, obtains fermentation raw materials;
[0039] d. Ferment the fermented raw material obtained in step c in a heat preservation device, set the in...
Embodiment 3
[0045] The present invention provides a kind of method that bacterium prepares biocontrol bacterial fertilizer, comprises the following steps specifically:
[0046] a. using the Bacillus subtilis isolated in the vineyard as a bacterial strain to carry out the isolation and identification of the bacterial species;
[0047] b. The corn material is sterilized by moist heat at 100Kpa and 130°C;
[0048] c. adding sterilized 3% soybean powder, 4% ammonium nitrate, 0.5% sucrose, 0.1% magnesium sulfate, 0.06% calcium sulfate, and 0.05% manganese sulfate to the carrier matrix of step b, And add the sterile water of carrier matrix weight 65% and the seed liquid of biocontrol bacteria of carrier matrix weight 1.2%, the seed liquid of biocontrol bacteria is the fresh bacteria liquid that storage time is no more than 36-48h, fully stirs evenly, obtains fermentation raw materials;
[0049] d. Ferment the fermented raw material obtained in step c in a thermal insulation device, set the in...
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