52 results about "Soil bacteria" patented technology

A method of production of granular soil conditioner based on a mixture of biochar and organic fertilizer comprising the following steps:Biochar mixing with animal excrements in a ratio of 1 part of biochar to 0.1 to 0.4 parts of the excrement dry matter, adding to the base of a mixture of soil bacteria belonging to at least two genera of the following: bacteria of the Rhizobia genus, nitrifying bacteria of the Azospirillum or Azotobacter genus, bacteria of the Pseudomonas genus, and bacteria of the Bacillus genus,topping the bacteria-endowed base with water in the weight not more than double the weight of the bacteria-endowed base and letting the mixture ferment for at least 5 days,draining off the excess water and drying the bacterial base so that the water content does not exceed 20% by weight,adding a mixture of spores of fungi containing at least two of the group including Trichoderma, Arbuscular mycorrhizal fungi, Ectomycorrhizal fungi to the base-bacteria compound in a total quantity corresponding to 0.2 to 1% of the dry matter weight of the base-bacteria compound.

The invention relates to the technical field of agricultural microorganisms, in particular to a microbial agent containing paenibacillus love KF-B6, a compound microbial fertilizer and application of the microbial agent and the compound microbial fertilizer. The KF-B6 Paenibacillus lovuenyuan as well as the microbial agent and the compound microbial fertilizer thereof provided by the invention can promote the formation of soil aggregates, improve soil organic matters, improve soil enzyme activity, effectively improve soil and increase the proportion of soil bacteria and fungi. Therefore, the method can be applied to prevention and treatment of apple continuous cropping obstacles, and the survival rate of field planting of apple seedling stocks and field planting of young trees in a rebuilt orchard is guaranteed. By applying the KF-B6 compound microbial fertilizer and the microbial agent, the survival rate of the M9T337 rootstock seedlings planted in the continuous cropping soil can be increased, the highest survival rate reaches 96.67%, and the seedling roots are high in activity and good in growth vigor. The survival rate of the Daphne red apple seedlings planted in the rebuilding of the continuous cropping obstacle old orchard is as high as 94.00%, and the effect of effectively preventing and controlling the apple continuous cropping obstacle is achieved.

The invention provides bacillus pseudomycoides BP88 and application of the bacillus pseudomycoides BP88. The preservation number of the bacillus pseudomycoides BP88 is CCTCC (China Center For Type Culture Collection) NO.20241800. The invention further provides a preparation method of the bacillus pseudomycoides BP88. The bacillus pseudomycoides has a good disease prevention effect on tobacco root rot and has a remarkable growth promoting effect on tobacco plants. The strain has good theoretical research value and application prospect, can be used as a good test material for researching interaction and action mechanism of soil bacteria and plants, can also be used for research and development of soil bio-fertilizers and biological bactericides, can also become a good carrier of multifunctional engineering bacteria for disease prevention, growth promotion and the like, and has broad application prospects. And a new strain resource is provided for biological prevention and control of tobacco root rot.

The invention discloses a growth-promoting bacterium and application thereof, and belongs to the technical field of microorganisms, the bacterial strain is classified and named as Phyllobacterium myrsinacearum, the bacterial strain number is B68, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31906. The invention further discloses a preparation method of the growth-promoting bacterium. The strain provided by the invention is separated from intergrassland soil of overgrazing sample plots, and is obtained by artificial separation and purification. The strain has high yield of 3-indoleacetic acid, has strong dissolving capacity for insoluble phosphate, and has good growth promoting characteristics of nitrogen fixation, ACC deaminase production, IAA production and the like. The prepared strain fermentation liquor is inoculated to leymus chinensis seedlings, and the strain can be colonized among the leymus chinensis roots to adjust the bacterial community structure of the soil among the leymus chinensis roots; growth of leymus chinensis is obviously promoted, photosynthesis is enhanced, and chlorophyll content is increased; the content of hormones and nutrients in plant leaves and roots is obviously improved; the contents of rapidly available phosphorus, total carbon and organic matters in rhizosphere soil are obviously improved. Therefore, the ardisia japonica phyllobacterium can be used for subsequent development and related research of microbial fertilizers for grazing grassland.

The invention provides a microbial fertilizer and a soil beneficial bacterium awakening method thereof. The microbial fertilizer comprises a hermetia illucens insect fertilizer, phosphate solubilizing bacteria, potassium solubilizing bacteria and nitrogen-fixing bacteria, the microbial fertilizer inoculant takes the hermetia illucens insect fertilizer as a basis and is supplemented with the phosphate solubilizing bacteria, the potassium solubilizing bacteria and the nitrogen-fixing bacteria; the phosphate solubilizing bacteria play a positive role in enhancing the phosphorus supply capability of the hermetia illucens insect fertilizer; the potassium bacteria play an important role in improving the potassium supply capability of the hermetia illucens insect fertilizer; the nitrogen-fixing bacteria have important significance for improving the nitrogen supply capability of the hermetia illucens insect fertilizer; the microbial fertilizer fungicide is guided to be used by a synergistic soil beneficial bacterium awakening method. The method comprises the following steps: detecting and knowing the basic conditions of the pH value, the organic matter content, the nutrient condition and the microbial community structure of soil in advance, and pretreating the soil to enable the soil to meet the conditions beneficial to microbial activity and plant root growth; a microbial fertilizer is applied according to the crop demand soil condition, a microbial agent is added to make full contact with the soil, and soil irrigation moisture preservation, monitoring and adjustment are kept.

The invention discloses a salt-alkali-tolerant growth-promoting ectopseudomonas DHS-HJ-IAA-2 strain and an application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of salt-resistant, alkali-resistant and drought-resistant pseudomonas sp. DHS-HJ-IAA-2 is screened, and the pseudomonas sp. DHS-HJ-IAA-2 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36164. Experimental results show that the ectopseudomonas DHS-HJ-IAA-2 has the functions of producing IAA, fixing nitrogen, dissolving potassium, degrading cellulose and lignin, inhibiting phytopathogen and the like, and can remarkably improve the bacteria abundance of saline-alkali soil, improve soil fertility and promote plant growth in a saline-alkali environment. The invention provides a new strain resource and a technical means for improving saline-alkali soil and planting crops on the saline-alkali soil, and realizes improvement of the saline-alkali soil from two aspects of soil-crop-microorganism comprehensive action and microorganism-plant synergistic action.

The invention provides bacillus mobilis BM84 and application of the bacillus mobilis BM84. The preservation number of the bacillus mobilis BM84 is CCTCC (China Center For Type Culture Collection) NO: M20241799. The bacillus mobilis has a good disease prevention effect on tobacco root rot and has a remarkable growth promoting effect on tobacco plants. The strain has good theoretical research value and application prospect, can be used as a good test material for researching interaction and action mechanism of soil bacteria and plants, can also be used for research and development of soil bio-fertilizers and biological bactericides, can also become a good carrier of multifunctional engineering bacteria for disease prevention, growth promotion and the like, and has broad application prospects. And a new strain resource is provided for biological prevention and control of tobacco root rot.

The utility model discloses a soil flora culture device with a split type bottom double-spiral belt and a reverse screw, and relates to a stirring device. The barrel bottom and the barrel body are mounted in the base; the lower end of the barrel body is communicated with the barrel bottom; the connecting cover is fixedly connected with the upper end of the barrel body; the barrel cover is connected with the connecting cover through a connecting device; the driving mechanism is fixed in the base; the stirring mechanism is mounted in the barrel body; two ends of the stirring mechanism are respectively connected with the barrel cover and the driving mechanism. According to the utility model, through the mutual matching among the outer-ring helical ribbon, the inner-ring helical ribbon and the connecting rod, the mixing and stirring of soil in the vertical direction, the cutting and mixing and stirring in the horizontal direction, the scraping of the inner wall of the barrel body and the filling of soil in a central hole in the barrel body can be realized; the stirring mechanism not only can realize rotary stirring around the central shaft, but also has upward thrust of a stirring rod and downward pressure of a helical ribbon, soil with the humidity of about 45% can be uniformly stirred, even large soil can be uniformly stirred, the stirring effect is strong, and the efficiency is high.

The application of "a unit type tidal wetland restoration method based on a target" belongs to the technical field of wetland ecosystem restoration methods. The unit type tidal wetland restoration method based on a target comprises the following steps: (1) determining a target; (2) constructing an ecological unit on the tidal wetland to be restored through microtopographic modification; (3) applying biochar materials and salt-resistant ecological materials in the ecological unit; and (4) configuring waterfront plants in the ecological unit. The restoration method utilizes natural tidal fluctuation to adjust the water and salt conditions of the ecological unit (salt reduction of more than 80%) and to maintain the target water level (0-2 m) as needed, optimizes the soil microbial community structure (soil bacterial richness is increased by more than 200%), improves the plant germination rate and survival rate in the process of tidal restoration (the germination rate is more than 90%, and the survival rate is increased to more than 62%), and enriches the plant configuration varieties in the riparian zone (≥12).

The invention discloses a fertilizer for increasing soil fertility of a cassava field. The fertilizer is prepared by applying 1 / 2 nitrogen-phosphorus-potassium compound fertilizer to cassava carbon soil; the fertilizing amount of the nitrogen-phosphorus-potassium compound fertilizer is the optimal dosage, and the cassava carbon soil is surface soil of a cassava planting zone after the cassava in season is harvested and smoldering products of waste cassava stems and leaves and old seed stems. The invention further discloses a fertilizing method for increasing the soil fertility of the cassava field. The fertilizing method comprises the following steps: preparing the nitrogen-phosphorus-potassium compound fertilizer; preparing cassava carbon soil; 1 / 2 of chemical fertilizer is reduced on the basis of recommended nitrogen-phosphorus-potassium fertilizer application, and the cassava carbon soil is applied in a matched mode; the cassava carbon soil is spread in a planting area before cassava planting, and the nitrogen-phosphorus-potassium compound fertilizer is applied in holes at a time 45-60 days after cassava planting. The method is beneficial to the recovery and improvement of soil nutrients and biological fertility, the richness and diversity of rhizosphere soil bacteria and fungi are obviously increased, the microbial community structure is obviously improved, the abundance of carbon and nitrogen metabolism functional genes is obviously improved, the investment of chemical fertilizers is reduced, and the yield loss caused by continuous cropping of cassava is effectively eliminated.

The invention discloses a controlled-release sugarcane antibacterial fertilizer granule and a preparation method and application thereof. The antibacterial fertilizer granule is prepared from the following raw materials in parts by weight: terpene alcohol 0.06-0.14%, tebuconazole 0.07-0.18%, fertilizer 30-65%, sugarcane leaves and defective fruit and vegetable fermentation 15-30%, coating agent 3.25-8%, and the remainder is auxiliary material. The preparation method includes preparing a fertilizer core, an inner coating layer, an organic fertilizer layer, and an outer coating layer. The present invention utilizes the principle of drug-fertilizer interaction, and according to the nutrient characteristics required for different growth periods of sugarcane, different types of fertilizers are compounded with terpene alcohol and tebuconazole, active ingredients of fungicides with antibacterial effects, and combined with coating slow-release technology, while meeting the nutrient requirements of sugarcane in different growth periods, taking into account the inhibition of soil harmful pathogens and the prevention of sugarcane diseases.

The invention belongs to the technical field of safety evaluation of gene editing plants, and particularly relates to a method for evaluating the influence of a gene editing plant on biodiversity. According to the method, the influence of the gene editing plant on community diversity of animals, plants and microorganisms on the ground and underground is comprehensively and accurately evaluated from the influence of the gene editing plant on arthropod community structures, the influence of the gene editing plant on occurrence of main weeds and the influence of the gene editing plant on the number of soil bacterial colonies and the community structures, and a parameter evaluation method is established; compared with a non-gene editing plant, the gene editing plant has no obvious influence on the community structure of arthropods in the field, the weed occurrence condition, the number of soil bacterial communities and the community structure. According to the evaluation method provided by the invention, the environmental safety risk of the gene editing plant can be systematically evaluated, and a solid technical support is provided for safety evaluation before commercialized planting of the gene editing plant.

The application discloses a rotary tillage mechanism with soil remediation function, and relates to the technical field of tillage equipment. The rotary tillage mechanism comprises a power system, a vehicle frame, a rotary tillage device and a high-voltage module. The rotary tillage device is arranged on the vehicle frame, and the power system drives the rotary tillage device to work. The high-voltage module and a plurality of dielectric barrier discharge devices are arranged below the vehicle frame. The dielectric barrier discharge devices intermittently ionize air to generate active substances and energy higher than corona discharge and spark discharge, so as to kill organisms in the soil. The high-voltage module provides a voltage of 5-8kV for the dielectric barrier discharge devices. The application can not only kill soil bacteria, but also effectively degrade residual pesticides in the soil, repair the soil structure and improve the growth environment of crop root systems, so as to ensure high yield of crops. The application is convenient for manual operation, saves time and effort, greatly improves production efficiency, reduces the labor intensity of farmers, and has the advantages of safety in use, no pollution to the environment and energy saving.

The invention belongs to the technical field of microorganisms, and particularly relates to ochrobactrum G3 and application thereof. The ochrobactrum G3 is classified and named as ochrobactrum and is preserved in the China Center for Type Culture Collection on November 17, 2025, and the preservation number of the ochrobactrum G3 is CCTCC NO: M 20252574. Soil bacteria are separated from rhizosphere soil of sorghum, antagonistic strains of the sorghum anthracnose are screened out, form and molecular identification of the strains is completed, the antibacterial spectrum of volatile organic compounds (VOCs) generated by the antagonistic strains and the control effect of fermentation bacteria liquid on sorghum are achieved, a new biocontrol resource is provided for prevention and treatment of the sorghum anthracnose, and the method has a wide application prospect. And a foundation is laid for further preventing and treating diseases by using the antagonistic strain.

The embodiment of the invention provides a soil bacterial community structure evaluation method and system based on an intermediate infrared spectrum transfer function and application, and relates to the technical field of soil microbial ecology. The method comprises the following steps: S1, collecting a soil sample; s2, acquiring a mid-infrared diffuse reflection spectrum of the soil sample to be detected; s3, acquiring an environment covariant corresponding to the soil sample, wherein the environment covariant comprises at least one of soil physicochemical properties, climate data and topographic parameters; s4, splicing the mid-infrared diffuse reflection spectrum obtained in the step S2 and the environment covariable obtained in the step S3 to form a fusion feature vector as an input feature set; and S5, inputting the input feature set into a pre-trained nonlinear machine learning regression model, and outputting the relative abundance and / or OTU richness of at least one bacteriophage in the soil sample. According to the method, a technical route of combining a mid-infrared (MIR) spectrum and a spectral transfer function (STF) is adopted and is used for predicting the soil bacterial community structure.

This invention relates to the field of agricultural microbiology, and in particular to a strain of *Bacillus erythropoietinus* KF-B6 and its applications, especially in the control of continuous cropping obstacles in apple orchards. The *Bacillus erythropoietinus* KF-B6, with preservation number CGMCC No. 32131, possesses a complex set of functions including rapid and high EPS (explosive peroxide) production, high chitinase production, production of water-soluble and volatile antibacterial substances, and decomposition of macromolecular organic matter. The application of this bacterium, its microbial agents, and compound microbial fertilizers can effectively increase the number of rhizosphere soil bacteria, reduce the number of pathogenic fungi, increase the ratio of bacteria to fungi, and promote the recovery of the soil microbial ecosystem in apple orchards with continuous cropping obstacles; increase the proportion of water-stable aggregates, increase soil organic matter content, and increase soil enzyme activity, thus improving the soil; improve the survival rate of saplings planted during the renewal of new varieties in old orchards with continuous cropping obstacles, promote root vitality and plant growth, and comprehensively alleviate and control continuous cropping obstacles in apple orchards.

The invention discloses a rhizosphere growth-promoting bacterium of sheep grass and its application, belonging to the field of microbial technology. The strain is classified and named as Phyllobacterium myrsinacearum, strain number B68, and deposit number CGMCC No.31906. The strain of the present invention is separated from the rhizosphere soil of sheep grass in an overgrazing sample, and is obtained by artificial separation and purification. The strain has high yield of 3-indoleacetic acid, has strong solubility for insoluble phosphates, and has good growth-promoting properties such as nitrogen fixation, ACC deaminase production, and IAA production. The prepared strain fermentation liquid is inoculated into sheep grass seedlings, and the strain can be colonized in the rhizosphere of sheep grass, regulate the bacterial community structure of rhizosphere soil of sheep grass; significantly promote the growth of sheep grass, enhance photosynthesis, and increase chlorophyll content; significantly increase the hormone and nutrient content in plant leaves and roots; significantly increase the content of available phosphorus, total carbon and organic matter in rhizosphere soil. Therefore, the myrsinacearum leaf bacillus of the present invention can be used for the development and related research of subsequent microbial fertilizers for grazing grasslands.

The present invention relates to the field of agricultural microbial technology, and in particular to a microbial agent and compound microbial fertilizer comprising Paenibacillus eihime KF-B6 and applications thereof. The KF-B6 Paenibacillus eihime and its microbial agent and compound microbial fertilizer provided by the present invention can promote the formation of soil aggregates, increase soil organic matter, increase soil enzyme activity, effectively improve the soil, and increase the proportion of soil bacteria and fungi. Therefore, it can be applied to the prevention and control of apple continuous cropping disorders to ensure the survival rate of planted apple seedling rootstocks and replanted saplings in orchards. The application of KF-B6 compound microbial fertilizer and agent can increase the survival rate of M9 T337 rootstock seedlings planted in continuous cropping soil, with the survival rate reaching up to 96.67%, and the root system of the seedlings is highly active and grows well. The survival rate of the Daphne Red apple seedlings replanted in old orchards with continuous cropping disorders is as high as 94.00%, achieving the effect of effectively preventing and controlling apple continuous cropping disorders.

The application discloses application of Enterobacter kobei in controlling and resisting evolution of soil bacteria antibiotic resistance. Enterobacter kobei LB1 is preserved in the China General Microbiological Culture Collection Center on May 15, 2026, and has a preservation number of CGMCC No. 38684 and a preservation address of No. 1, Xibaixili, Chaoyang District, Beijing, China Institute of Microbiology. Enterobacter kobei LB1 and extracellular metabolites have the ability of controlling and resisting evolution of soil bacteria antibiotic resistance. The application verifies the inhibiting effect of the resistance mutant strain of the control agent through a soil microcosm experiment, realizes effective intervention on the evolution of soil microbial resistance, and has the advantages of green ecology, safety, high efficiency, long-term control and the like, and provides a new technical way for environmental antibiotic resistance pollution treatment.

The invention provides high-humus coal gangue-based bionic peat as well as a preparation method and application thereof, the preparation method comprises the following steps: (1) inoculating a soil bacterial suspension into a lignin screening liquid culture medium for enrichment culture to obtain a high-temperature-resistant bacterial solution H1; and (2) transferring the high-temperature-resistant bacterial liquid H1 into a coal gangue and garden waste mixed substrate culture medium, adding Tween 80 for fermentation, and drying to obtain the high-humus coal gangue-based bionic peat, the fermentation time is 20 to 35 days. According to the preparation method disclosed by the invention, the coal gangue-based bionic peat with high humus content can be simply and quickly obtained, the high-humus coal gangue-based bionic peat can be used for repairing various soils, and resource utilization is realized while the problem that a large amount of coal gangue and garden wastes are accumulated is solved.

The invention belongs to the technical field of microorganisms, and particularly relates to a complex microbial inoculant for relieving phosphorus deficiency in the seedling stage of corn covered with straw and application of the complex microbial inoculant. Bacillus subtilis, saccharomycetes and pseudomonas fluorescens are compounded into the complex microbial inoculant, saccharomycetes secrete IAA and siderophores to promote the root development of seedlings, through domestication culture, the adaptability of the microbial inoculum in a straw-covered low-temperature environment is enhanced, the activation capacity on phosphorus is improved, the phosphorus nutrition level is synergistically improved in cooperation with bacillus subtilis, pseudomonas fluorescens can enhance the resistance of the seedlings to low-temperature stress, and the survival rate of the seedlings is increased. Soil-borne pathogenic bacteria which cannot be effectively inhibited by some soil florae are specifically inhibited, the influence of harmful bacteria on the activation effect of phosphorus is reduced, the three strains are matched to remarkably improve the content of rapidly available phosphorus in soil, reduce the incidence rate of phosphorus deficiency symptoms and improve the stress resistance and growth indexes in the corn seedling stage, and the compound microbial agent is suitable for a straw covering cultivation mode.

The invention provides a bacillus arachzii BA16 and application thereof, the bacillus arachzii BA16 is named as bacillus arachzii BA16 in taxonomy, and the preservation number of the bacillus arachzii BA16 is CCTCC (China Center for Type Culture Collection) NO.20241881. The peanut bacillus has a good disease prevention effect on tobacco root rot and has a remarkable growth promoting effect on tobacco plants. The strain has good theoretical research value and application prospect, can be used as a good test material for researching interaction and action mechanism of soil bacteria and plants, can also be used for research and development of soil bio-fertilizers and biological bactericides, can also become a good carrier of multifunctional engineering bacteria for disease prevention, growth promotion and the like, and has broad application prospects. And a new strain resource is provided for biological prevention and control of tobacco root rot.

The invention discloses a vegetation-soil-microorganism synergistic remediation treatment method for a stony desertification slope, and relates to the technical field of soil remediation, and the method comprises the following steps: S1, precise evaluation of site conditions: uniformly arranging 10m * 10m quadrats on a target slope by adopting a GPS positioning and field sampling combined method, and measuring the slope, rock exposure rate, soil thickness and soil organic matter content (SOM); s2, vegetation three-dimensional configuration and field planting: selecting local stress-tolerant species, and constructing a three-dimensional community according to a plant / cluster number ratio of (2-4): (2-4): (3-5); according to the method, vegetation-soil-microorganisms are regarded as an organic whole, a synergistic mutual promotion mechanism of the vegetation, the soil and the microorganisms is systematically constructed, the vegetation fixes a carbon source through photosynthesis, root exudates nourish the microorganisms, the soil provides a physical carrier and a basic environment for the vegetation and the microorganisms, and the microorganisms supply energy to the vegetation through nutrient conversion; and a forward circulation of vegetation soil fixation, soil bacterium cultivation and microbe vegetation promotion is formed.

The invention discloses a polymyxa Paenibacillus, which belongs to the technical field of agricultural microorganisms. The invention isolates a polymyxa Paenibacillus GRY‑11 from healthy apple rhizosphere soil in a continuous cropping orchard, belonging to Paenibacillus polymyxa. Experiments have shown that the strain has antagonistic effects on Fusarium oxysporum, Fusarium moniliforme, Fusarium laminarum, and Fusarium solani that cause apple continuous cropping obstacles; the strain can increase the number of soil bacteria, reduce the number of pathogens, and promote plant growth; improve soil enzyme activity in apple continuous cropping soil and protective enzyme activity of apple seedling roots; reduce the content of soil phenolic acid substances; and effectively alleviate apple continuous cropping obstacles.

The application relates to the field of agricultural technology, and particularly discloses a disease soil bacteria detection and fertilizer application repair and improvement method and application thereof. The disease soil bacteria detection and fertilizer application repair and improvement method provided by the application comprises the following steps: (1) in October of the current year, the organic matter content in disease soil is detected, and the soil conditioner dosage is determined; in November, the soil conditioner is applied to the disease soil; (2) in early May of the next year, the relative abundance of fungal and bacterial genus level pathogenic bacteria in normal soil and disease soil is detected, and the microbial agent dosage is determined; in mid-May, the microbial agent is applied to the disease soil. The disease soil bacteria detection and fertilizer application repair and improvement method provided by the application dynamically adjusts the microbial agent dosage by detecting and analyzing the microbial structure difference between disease soil and normal soil in the crop growth process, so that the microbial system of the disease soil is improved, and the repair of the heavy-rotation and continuous-cropping obstacle soil is realized.

This invention proposes a method for reducing cadmium in paddy fields using common soil microorganisms, belonging to the field of agricultural technology. Addressing the problems of high cost, secondary pollution, and soil structure damage associated with existing physicochemical cadmium reduction methods, this invention provides a bioremediation technology using Bacillus subtilis ATCC6633 as the functional strain. This method involves activating laboratory-preserved Bacillus subtilis ATCC6633 bacterial powder to prepare a microbial agent, which is then directly applied to the surface water of paddy fields. Utilizing the specific adsorption and conversion of cadmium ions by the strain, effective passivation and removal of cadmium from paddy fields are achieved. This invention uses common soil microorganisms, exhibiting strong environmental compatibility and eliminating the need for chemical agents, fundamentally avoiding the secondary pollution problems caused by traditional chemical precipitation methods. It is both eco-friendly and economically feasible, significantly improving rice quality and providing an effective green remediation approach for cadmium pollution in paddy fields.

The invention discloses beneficial complex microbial inoculant powder, a special bio-organic fertilizer for early rice and a preparation method of the special bio-organic fertilizer, and relates to the technical field of bio-organic fertilizers. The invention relates to a microbial organic fertilizer, which is prepared from the following components in percentage by mass: 40 to 50 percent of iron oxidizing bacteria (Sideroxydans), 15 to 25 percent of ammonia oxidizing bacteria (MND1), 15 to 25 percent of thiobacillus (Thiobacillus) and 5 to 10 percent of pseudomonas (Pseudomonas), and can be used for promoting the yield increase of crops, fertile soil and acid reduction and improving the ecological function of a farmland. The invention further discloses application of the beneficial complex microbial inoculant powder to preparation of the special bio-organic fertilizer for early rice. The prepared special bio-organic fertilizer for early rice can obviously improve the fertilizer retention and supply properties of soil and the pH value of the soil; the abundance of soil bacterial microflora is improved, the microbial diversity indexes of bacteria Chao1, Shannon and the like are increased, and the nutrient circulation rate is accelerated; soluble organic carbon, microbial biomass carbon and nitrogen cycle related enzyme activity of soil are enhanced, and high rice yield and soil quality index are maintained.

A method for producing a granular soil conditioner based on a mixture of biochar and organic fertilizer comprises the following steps: - mixing biochar with animal waste in a ratio of 0.1-0.4 parts by weight of waste dry matter to 1 part by weight of biochar and adding to this substrate a mixture of soil bacteria belonging to at least two of the following genera: Rhizobium, Azospirillum or Azotobacter nitrifying bacteria, Pseudomonas and Bacillus; - supplementing the bacteria-containing substrate with water up to twice the weight of the bacteria-containing substrate and fermenting the mixture for at least 5 days; - pouring in an excess of water and drying the bacterial substrate so that the moisture content is less than 20% by weight; - adding to the substrate-bacteria compound a mixture of fungal spores including at least two species from the groups Trichoderma, arbuscular mycorrhizal fungi and ectomycorrhizal fungi in a total amount corresponding to 0.2-1% by weight of the dry matter weight of the substrate-bacteria compound.

This invention belongs to the field of plant rhizosphere research and discloses a method for determining the regulatory effect of exogenous selenium application on the rhizosphere microecology of Amorphophallus bulbifera. The method includes: experimental design and sample collection; soil microbial DNA extraction and amplicon sequencing; and quality control and analysis of the microbiome data. This invention utilizes Illumina MiSeq high-throughput sequencing technology to analyze the changes in the composition and diversity of rhizosphere soil bacteria communities in Amorphophallus bulbifera under exogenous selenium treatment, and further analyzes the functions of related communities through PICRUSt functional prediction analysis. The conclusions are as follows: exogenous selenium treatment induces significant changes in the rhizosphere soil bacterial community of Amorphophallus bulbifera, which is beneficial for creating a stable and more diverse rhizosphere microbial community structure.