7 results about "Soil bacteria" patented technology

The application relates to the field of agricultural technology, and particularly discloses a disease soil bacteria detection and fertilizer application repair and improvement method and application thereof. The disease soil bacteria detection and fertilizer application repair and improvement method provided by the application comprises the following steps: (1) in October of the current year, the organic matter content in disease soil is detected, and the soil conditioner dosage is determined; in November, the soil conditioner is applied to the disease soil; (2) in early May of the next year, the relative abundance of fungal and bacterial genus level pathogenic bacteria in normal soil and disease soil is detected, and the microbial agent dosage is determined; in mid-May, the microbial agent is applied to the disease soil. The disease soil bacteria detection and fertilizer application repair and improvement method provided by the application dynamically adjusts the microbial agent dosage by detecting and analyzing the microbial structure difference between disease soil and normal soil in the crop growth process, so that the microbial system of the disease soil is improved, and the repair of the heavy-rotation and continuous-cropping obstacle soil is realized.

This invention proposes a method for reducing cadmium in paddy fields using common soil microorganisms, belonging to the field of agricultural technology. Addressing the problems of high cost, secondary pollution, and soil structure damage associated with existing physicochemical cadmium reduction methods, this invention provides a bioremediation technology using Bacillus subtilis ATCC6633 as the functional strain. This method involves activating laboratory-preserved Bacillus subtilis ATCC6633 bacterial powder to prepare a microbial agent, which is then directly applied to the surface water of paddy fields. Utilizing the specific adsorption and conversion of cadmium ions by the strain, effective passivation and removal of cadmium from paddy fields are achieved. This invention uses common soil microorganisms, exhibiting strong environmental compatibility and eliminating the need for chemical agents, fundamentally avoiding the secondary pollution problems caused by traditional chemical precipitation methods. It is both eco-friendly and economically feasible, significantly improving rice quality and providing an effective green remediation approach for cadmium pollution in paddy fields.

This invention belongs to the field of plant rhizosphere research and discloses a method for determining the regulatory effect of exogenous selenium application on the rhizosphere microecology of Amorphophallus bulbifera. The method includes: experimental design and sample collection; soil microbial DNA extraction and amplicon sequencing; and quality control and analysis of the microbiome data. This invention utilizes Illumina MiSeq high-throughput sequencing technology to analyze the changes in the composition and diversity of rhizosphere soil bacteria communities in Amorphophallus bulbifera under exogenous selenium treatment, and further analyzes the functions of related communities through PICRUSt functional prediction analysis. The conclusions are as follows: exogenous selenium treatment induces significant changes in the rhizosphere soil bacterial community of Amorphophallus bulbifera, which is beneficial for creating a stable and more diverse rhizosphere microbial community structure.

This invention discloses the application of isocitric acid in the control of bacterial wilt in plants, wherein the isocitric acid is derived from tomato root exudates. The inhibitory effect of isocitric acid on *Ralstonia solanacearum* was evaluated through shake-flask culture experiments and plate inhibition zone experiments, and the control effect of isocitric acid on bacterial wilt was determined using greenhouse pot experiments. The results showed that within the concentration range of 10–200 μM, isocitric acid significantly inhibited the growth of *Ralstonia solanacearum*, forming a distinct inhibition zone. Under greenhouse pot conditions, applying 0.2–0.5 mg / kg of isocitric acid to the soil effectively reduced the incidence of bacterial wilt in tomatoes, with a disease control rate of 21.43%–52.38%. Furthermore, isocitric acid can indirectly inhibit the occurrence of bacterial wilt by influencing the soil bacterial community. The isocitric acid used in this invention is derived from natural plant root exudates, possessing advantages such as environmental friendliness and high safety, and can be used as a novel inhibitor of tomato bacterial wilt, showing promising application prospects in the field of green control of plant diseases.

The application discloses a kind of full solid waste base biological fiber cooperates microorganism mineralization flow state solidified soil and preparation method thereof.The application is immersed in alkaline solution by biological base fiber, and modified carrier material is obtained;Waste concrete or waste brick ball is crushed and ball milled, and recycled micro-powder cementitious material is prepared;Modified carrier material is mixed with engineering waste, and recycled micro-powder cementitious material is dry mixed, and urease-producing bacterial solution or calcium carbonate precipitation-inducing microbial solution is added to stirring and standing, and mixture is obtained;Urea and calcium source are contained in the mixture and added to cementing fluid, and stirring is obtained, and the full solid waste base biological fiber flow state solidified soil is obtained.The application utilizes the alkaline excitation of recycled micro-powder, the carrier anchoring of biological fiber and the suspension synergistic effect of biological glue, effectively solves the problems of traditional MICP flow state soil bacteria easy loss, cement-free system easy segregation and large brittleness, and the prepared filler has the characteristics of high flowability, high toughness and low carbon environmental protection.