75 results about "Soil bacteria" patented technology

The invention relates to a liquid microbial fertilizer with nitrogen-fixing, phosphorus-dissolving and potassium-dissolving effects, and a preparation method thereof. The liquid microbial fertilizer consists of the following microbial fermentation liquids in parts by volume: 1 to 10 parts of azotobacter chroococcum, 1 to 30 parts of bacillus mucilaginosus, 1 to 10 parts of phosphorus-dissolving bacillus megatherium, 1 to 10 parts of bacillus amyloliquefaciens, 1 to 10 parts of bacillus subtilis, 1 to 5 parts of photosynthetic bacteria and 1 to 10 parts of streptomyces microflavus. The functional bacteria serve as main functional flora of the microbial fertilizer, so that the obtained product has remarkable effects of fixing nitrogen, dissolving phosphorus, dissolving potassium and resisting disease, can enhance the total metabolic activity of soil bacterial community and the diversity of the bacterial community, improve the soil micro-ecological environment, promote growth and reproduction of beneficial microorganisms and inhibit the growth of pathogenic bacteria, is an ideal fertilizer in green agriculture and organic agriculture, and has a wide prospect in agricultural sustainable development.

The invention discloses a method for killing soil bacteria and insects and improving soil and a special device for killing bacteria and insects, wherein the method for killing soil bacteria and insects and improving soil comprises the following steps: first, rotatably plowing the surface soil, where the rotary plowing depth is not less than 35 cm; second, shoveling the surface soil from the land, and lifting the shoveled surface soil and then baking the surface soil by flame; and third, complementing microorganism, compost and trace element; the special device comprises a box, wherein a shoveling plate is arranged in the front of the box body; a soil inlet is arranged on the front wall of the box body; a transmission shaft is arranged above the back of the shoveling plate; soil-lifting blades are distributed on the transmission shaft annularly; a first tilting plate is arranged on the middle lower part of the box body; a gas pipe is arranged at the top of the box body; and a flame stove is arranged on the box body. When the special device and the method disclosed by the invention are used for killing bacteria and insects, the cost is low, the insect-killing effect is good and the organic activity and microorganism activity in the soil are ensured.

A biodegradable, non-toxic firefighting concentrate composition. The preferred compositions include a mixture of a C14-C18 5 mole ethoxylated tallow amine, 2 mole ethoxylated coconut amine, and 5 mole ethoxylated coconut amine, wherein the blend is neutralized with a C6 carboxylic acid; an aliquot of 33 parts is added to 3 parts of a 2 mole ethoxylated linear alcohol having 6 to 16 carbon atoms, and enough water to bring the total weight percentage to 70%. Ten to fifteen parts of sodium lauryl sulfate is added and the balance of water is added to bring the total up to 100%. The composition is also effective when mixed with glycol dimethyl ether, the incorporation of a filming protein based agent, dye, or foam forming materials. In addition, the composition is useful with soil bacteria for remediating soil contaminated with hydrocarbon fuel and for facilitating fuel dispersion and degradation within bacterial-action sewage systems.

The production process of liquid seaweed fertilizer includes the steps of soaking kelp in fresh water, crushing or shearing kelp, degrading kelp with acid to produce seaweed polysaccharide with polymerization degree controlled below 100, digesting the degraded liquid with alkali matter, filtering and concentration. The liquid seaweed fertilizer thus produced contains the nutritive components of seaweed as well as active components algin oligose, plant growth regulator and great amount of trace elements P, Ca, K, Mg, Zn and Fe. It may be used as foliage fertilizer and base fertilizer and canpromote the growth of bacteria in soil, and is easily absorbed by crop root system to promote plant's growth, raise disease resistance and improve crop quality.

The invention relates to an efficient flocculating agent for rapidly removing heavy metals in water. The efficient flocculating agent comprises a mixture A and a mixture B, wherein the mixture A comprises the following components in parts by mass: 100 parts of No.2 Hangjin soil, 5-10 parts of soil bacteria and 15-25 parts of activated carbon; and the mixture B comprises the following components in parts by mass: 10-20 parts of iron oxide, 10-20 parts of iron chloride, 20-50 parts of poly-aluminum chloride, 2.5-5 parts of an organic flocculating agent, 25-50 parts of cement and 16-32 parts of sodium thiosulfate. The efficient flocculating agent disclosed by the invention can be used for rapidly and effectively removing a variety of heavy metals such as mercury, lead, chromium, cadmium, arsenic and copper in the water, is short in reaction time, cannot cause secondary pollution, can be directly fed without early dissolution, is simple in use method, and can be widely applied to removal of the heavy metals in various industrial wastewater, polluted underground water, lakes, rivers and reservoirs.

Mycorrhizal and rhizobial associations represent the two most important symbiotic relationships between higher plants and microorganisms, providing access to otherwise limiting supplies of phosphate and nitrogen, respectively. Although many higher plants are able to establish a symbiotic relationship with arbuscular mycorrhizal fungi, legumes are unusual among plants because they also form associations with nitrogen fixing soil bacteria called rhizobia. This symbiosis requires the production of bacterial signals, “Nod factors” that trigger several key developmental responses in the host plant (Dénarié et al., 1996). The DMI1 gene of the model legume M. truncatula plays a major role both in the early steps of Nod factor signaling and in the establishment of mycorrhizal symbiosis. Dmi1 mutants do not exhibit many of the early responses to Nod factors and are incapable of forming nitrogen fixing root nodules. Here we describe the cloning and preliminary characterization of DMI1. The DMI1 gene encodes a novel protein with low global similarity to ligand-gated cation channels of archaea. The protein is highly conserved in angiosperms and ancestral to land plants. Interestingly a putative A. thaliana DMI1 orthologous gene is expressed in roots. As A. thaliana is unable to establish a mycorrhizal symbiosis, this finding suggests that DMI1 may also exhibit a function that is independent of symbiotic interactions.

The invention relates to fertilizer compositions comprising a carbon source and a source of nitrogen. Carbon is in form that can be readily up-taken by soil bacteria. The invention also relates to a method for manufacturing the fertilizer, a method for fertilizing soil and uses of said fertilizer. Use of the fertilizer promotes uptake of endogenous soil nutrient resources by plant.

An agriculturally effective active ingredient is applied to plant foliage before, after, or simultaneously with an enhancer component containing a substantially pure bacterial culture, suspension, spores, or cells of a bacteria selected from the genus Bacillus or a soil bacteria.

The invention discloses a method and equipment for killing bacteria and pests in soil of a greenhouse. The prior method for killing soil bacteria and viruses as well as underground pests by chemical agents fails to achieve ideal sterilizing and disinsectizing effects. The method comprises the following steps: high-temperature gas produced by fuel (5) in a baking oven passes through a hot air pipe (7), a hot air hole (9) and a hot air chamber (8), and enters the soil through rake ditches formed by rake teeth (10) to kill the bacteria and the pests in the soil of the greenhouse. The method has the advantages of killing the bacteria in the soil of the greenhouse and having low pest cost as well as good sterilizing and disinsectizing effects. The equipment has the advantages of simple structure, small volume and convenient operation, and is suitable to be popularized and applied in the greenhouse. The method smartly utilizes the rake ditches formed by operating the rake teeth to ensure that the high-temperature gas evenly enters the soil to combine raking land with sterilization and disinsectization without drilling holes to the soil additionally, thereby saving labor and time, and having good effect at the same time.

The invention relates to the field of bacterial experimental equipment, in particular to an experimental apparatus for soil bacteria isolation and enumeration, comprising an apparatus body, a stock solution preparation unit, a diluting unit, a conveying unit, a coating unit, a marking unit, a sterilizing unit, a drying unit, a water supply unit and a control unit; the stock solution preparation unit is used for preparing a solution of bacteria in soil, the diluting unit is used for diluting stock solution in gradient manner, the conveying unit is used for conveying a culture dish, the coating unit is used for coating a culture medium, the marking unit is used for marking the culture dish, the drying unit is used for keeping the inside of the apparatus body dry, and the water supply unit is used for supplying aseptic distilled water. The traditional manual isolation and enumeration method for soil bacteria is replaced, the experimental cycle is shortened, labor intensity of experimenters is reduced, and disturbances on experimental results due to the differences of operating skills of experimenters are avoided; in addition, the apparatus has no need for use with an ultra-clean workbench, and experimental equipment resources are saved.

The invention discloses a high-throughput absolute quantification method for soil bacteria. The high-throughput absolute quantification method includes steps of whirling and re-suspending E. coli HTAQ-GFP thalli in sterile water and regulating bacteria suspension in ultraviolet spectrophotometers OD <600nm=1.0>; 2), absolutely quantifying internal standard strains HTAQ-GFP and acquiring the absolute content of internal standard strains E. coli HTAQ-GFP in bacteria solution; 3), adding the bacteria solution with the internal standard strains HTAQ-GFP to soil samples and uniformly stirring and mixing the bacteria solution and the soil samples with one another; 4), extracting soil DNA (deoxyribonucleic acid) and carrying out high-throughput sequencing on amplicons of 16S rRNA [16S ribosomal RNA (ribonucleic acid)] genes to obtain classification composition information of bacterial community structures and corresponding relative abundance; 5), acquiring the total quantities of native bacteria in soil and the absolute contents of various classification units according to the absolute contents of the internal standard strains HTAQ-GFP and the relative abundance of Escherichia in high-throughput sequencing results.

The invention discloses corrosion and scale inhibition biodegradable bactericide used for water treatment. The bactericide is prepared from 5-30% of hexadecyl dimethyl (2-sulphurous) ethyl ammonium, 5-20% of acrylic acid-allyloxy polyether-acrylate copolymers, 5-30% of polyquaternium, 2-25% of polyaspartic acid and 25-50% of water. The 28-day degradation rate of the bactericide reaches 90%, and the bactericide has a good bactericidal effect on microorganism soil bacteria, sulfate reducting bacteria, iron bacteria and heterotrophic bacteria, has the corrosion and scale inhibition functions, has a good dispersion effect on calcium carbonate scale and is environment-friendly bactericide used for water treatment.

The invention discloses a soil conditioner for tobacco field acidic soil, wherein the soil conditioner comprises, by weight, 40-80 parts of shells, 20-60 parts of potassium feldspar, 10-50 parts of limestone, and 15-40 parts of dolomite. According to the present invention, the soil conditioner can neutralize the active acid and the exchangeable acid in the soil, obviously increase the pH value ofthe soil, increase the soil plough layer exchangeable Ca<2+> and Mg<2+> concentrations, improve the soil microbial environment, increase the numbers of soil bacteria, actinomycetes and aerobic cellulose decomposition bacteria, improve the activities of urease, acid phosphatase, catalase and cellulase, passivate the heavy metals in the soil, reduce the heavy metals in tobacco, reduce the occurrenceof tobacco root diseases, improve the aroma quality, the aroma amount and the concentration of tobacco leaves, and reduce the irritation, the miscellaneous gas and the like of tobacco leaves.

The invention belongs to the field of biological bacterial fertilizers, in particular to a composite microbial agent. The composite microbial agent is prepared from five types of beneficial soil bacteria, wherein bacillus subtilis (TMQK07), bacillus laterosporus (TMQC01) and lactobacillus casei (TMQR02) are mixed according to a living bacteria count proportion of (0.9 to 1) to (0.8 to 0.9) to (5 to 6), and a live bacteria count is larger than or equal to 3,000,000,000 / ml; and moreover, streptomyces griseus (TMQMH05) fermentation liquor containing 700 U / ml or above of streptomycin, and trichoderma harzianum (TMQMH12) fermentation liquor with a spore count of 500,000,000 / ml are mixed with a mixture of the bacillus subtilis, the bacillus laterosporus and the lactobacillus casei, and polysaccharides, gamma-polyglutamic acid and humic acid are added as synergists in order to overcome the continuous cropping of crops, lower the incidence rate of wilt, and achieve the effects of resisting bacteria, tonifying plants, preventing diseases and improving yield.

A method of mixing organic carbon with the final effluent for water treatment systems and applying the mixture to manmade stratified sand filters or natural soils to induce proper conditions for high removal of total nitrogen in the final effluent. In one embodiment, water having a concentration less than about 30 mg / l of Total Nitrogen (TN) and a BOD5 concentration of less than about 30 mg / l is mixed with a sufficient amount of dissolved carbon to cause a reduction of the nitrogen pollution in the water, and the water and dissolved carbon are percolated through a fixed bed.A method of mixing organic carbon with any water having dissolved TN and applying the mixture to manmade stratified sand filters or natural soils to induce proper conditions for high removal of total nitrogen in the applied water.A method of mixing organic carbon with any water having dissolved TN and applying the mixture to manmade stratified sand filters and applying the water to the soils and inducing in situ denitrification caused by the discharge of water having the proper Carbon to Nitrogen ratio for soil bacterial metabolism and by the discharge of long chained insoluble particular carbon, which degrades in the soils and soil water and provides carbon for denitrification.

The invention discloses primers for identifying pathogenic bacteria of tomato neck-root rot and wilt and a kit. The primer sequences for identifying pathogenic bacteria of tomato neck-root rot are shown as SEQ ID NO: 1 and SEQ ID NO: 2, and the primer sequences for identifying pathogenic bacteria of tomato wilt are shown as SEQ ID NO: 3 and SEQ ID NO: 4. The primers are strong in specificity and high in detection accuracy, can be used for detecting No. 1 physiological races of fusarium oxysporum tomato neck-root rot specialis and fusarium oxysporum tomato wilt specialis, and is significant for early quick detection of tomato neck-root rot and wilt, soil bacteria community distribution as well as prevention and control of diseases.

The invention provides a preparation method and an application of perfume for a commelina communis cigarette. The perfume is prepared by the method comprising the following steps: (1) preparing a cigarette tobacco yellow soil bacteria X7X bactericide; (2) fermenting commelina communis powder by using the cigarette tobacco yellow soil bacteria X7X bactericide, to obtain fermented commelina communispowder; (3) heating and reflux-extracting the fermented commelina communis powder, filtering and concentrating to obtain commelina communis concentrated extracting solution; and (4) fine-extracting the commelina communis concentrated extracting solution through macroporous resin, to obtain the perfume for the commelina communis cigarette. The perfume for the commelina communis cigarette is applied to a cigarette tobacco shred. In a smoking process of the cigarette, special herb fragrance and faint scent coordinated with fragrance of the cigarette can be generated, the fragrance of the cigarette is enriched, and the fragrance is softer and smoother. Remaining taste is clean, and smoking quality of the cigarette is improved.

The invention discloses a method for treating fresh food garbage in kitchens by utilizing microbial decomposition, which relates to garbage decomposition treatment technique. In the method, zeolite and an active agent are added to a bacteria bed to form the raw material, wherein the bacteria bed is formed by culturing buckwheat husk with soil bacteria and Bacillus subtilis without using pesticide; the zeolite should be used after being heated, and has the effects of deodorization, sterilization and gas absorption; and the active agent can promote the activity of microbes, widen the garbage decomposition range and increase the garbage decomposition rate. The method mainly comprises the following steps: putting the fresh food garbage in, stirring, decomposing and discharging water. The method can not pollute the environment in the process of treating the fresh food garbage, and the fresh food garbage can be treated on site and can not be moved to other places; and thus, the invention meets the requirements of novelty and environmental protection for garbage treatment currently.

The invention discloses an agricultural multifunctional biological bacterial fertilizer, comprising a pesticide and composite biological flora at the ratio is 1,000:(10-20) in parts by weight, wherein the viable count of the composite biological flora is a billion per gram; the composite biological flora comprises bacillus subtilis, bacillus megatherium, conidioblous thromboides and phosphate and potassium-solubilizing nitrogen-fixing bacteria; the pesticide is dinotefuran, thiamethoxam or imidacloprid. Fertilization can be carried out at one time by the synergistic effects of biological bacterium and pesticide, the effect of improving good soil bacteria, killing and preventing soil insects and a part of insects on the floor, promoting root seedlings, preventing diseases, increasing yield and the like in the growth processes of a plurality of crops are achieved, the drug application time is saved, and the agricultural multifunctional biological bacterial fertilizer is convenient to use.

The present invention discloses a composite fertilizer special for tomatoes, which comprises the following raw materials in part by weight: 10-15 parts of ammonium molybdate, 20-25 parts of diammonium phosphate, 20-30 parts of potassium chloride, 1-4 parts of zinc borate, 5-10 parts of potash magnesium sulphate fertilizer, 1-2 parts of manganese sulfate, 1-3 parts of zinc-amino acid chelate, 1-2 p,arts of iron-amino acid chelate, 1-4 parts of copper-amino acid chelate, 2-3 parts of molybdenum-amino acid chelate, 30-40 parts of pond sludge, 15-20 parts of corn cakes, 20-25 parts of rape seed cakes, 30-40 parts of fully rotten byre manure, 30-40 parts of hog manure, 1-2 parts of borax, 10-15 parts of peat soil, 2-5 parts of attapulgite, 2-3 parts of nano-river sand powders, 2-5 parts of phosphorus rock powders, 0.5-0.8 part of naphthalene acetic acid, 0.05-0.1 part of gibberellin, 1-2 parts of saccharomycetes and 2-4 parts of soil bacteria. The composite fertilizer special for tomatoes is rich in nutrients, good in slow-release effect, green and environment-friendly, which can improve the soil.

The invention discloses a method for detecting the number of soil bacteria based on real-time fluorescent quantitative PCR, and belongs to the technical field of biology. The method comprises the following steps: 1) extracting soil sample total DNA and adjusting the total DNA to a preset concentration to prepare a to-be-detected soil sample template DNA solution; 2) extracting the total DNA of template escherichia coli, measuring the DNA concentration, and preparing a standard curve template DNA solution; 3) carrying out real-time fluorescent quantitative PCR amplification on the to-be-detected soil sample template DNA solution and the standard curve template DNA solution; 4) drawing a standard curve; and 5) calculating the number of bacteria per gram of dry soil. According to the method,the steps of adding an internal standard strain for fluoroscopy and the like are omitted, the operation difficulty is greatly reduced, connection, conversion and qPCR amplification are not needed, operation is easy, convenient and easy to learn, and the target fragment is good in specificity, high in data accuracy and good in reproducibility.

The present invention discloses nutrient soil for radish planting. The nutrient soil is prepared from the following dry powder of raw materials in parts by weight: 50-70 parts of pond mud, 20-25 parts of corn cobs, 10-15 parts of longan cores, 5-10 parts of banana peel, 5-10 parts of camellia seed, 3-5 parts of rosa laevigata michx and 1-2 parts of ganoderma. The nutrient soil disclosed by the present invention is hign in fertilizer efficiency, and various nutrient elements are added into soil property. The nutrient soil can inhibit the growth of soil bacteria.

Stable, liquid fertilizer compositions comprising nickel, cobalt, and molybdenum are provided herein. The fertilizer compositions are capable supplying high levels of micronutrients to seeds at low doses of the compositions. The compositions generally have low salinity and a pH from about 6.0 to about 8.0, thereby avoiding the high mortality rates of beneficial soil bacteria, which has been a major problem with prior seed fertilizers. Also provided herein are methods of forming and using the fertilizer compositions. Notably, it has been discovered that monoethanolamine molybdate solutions can be used to provide high levels of molybdenum to seed surfaces in a stable liquid formulation.

The invention discloses a solid flower fertilizer formula and process. The formula ratio is 1-2 parts of potassium nitrate, 1.2-1.5 parts of calcium superphosphate, 0.5-0.9 parts of calcium nitrate, 0.8-1.5 parts of magnesium sulfate, and 0.01-0.1 parts of iron sulfate , 0.01-0.05 parts of manganese sulfate, 0.001-0.01 parts of borax, 0.001-0.005 parts of zinc sulfate, 0.001-0.005 parts of copper sulfate; can make full use of planting time, planting flowers are not limited by seasons, and can be carried out in any season; Invaded by bacteria in the soil, no pests and diseases occur, so there is no need to use pesticides to avoid pesticide pollution; flowers can grow luxuriantly without removing clutter and impurities; there will be no mildew and rotten smell caused by fertilization; dedicated to The cultivation of various flowers can also be used for the cultivation of various vegetables.

The invention relates to a method for improving soil physical and chemical properties of continuous cropping crops in a greenhouse. Under sunlight illumination, the temperature and humidity of the greenhouse are rapidly increased, the air temperature and the ground temperature can reach 60 DEG C and 40 DEG C, and the temperature increase range is about 10-20 DEG C, which is maintained for a certain period of time so as to kill soil bacteria and improve a soil structure; the community structure of plant root microorganisms is improved, beneficial bacteria becomes dominant strains, and a soil environment is effectively improved; soil salt accumulation is reduced, soil physical and chemical properties are improved, and soil nutrients are balanced; an enzyme activity is reduced; after water pouring high temperature greenhouse suffocation treatment, a certain repairing effect on greenhouse continuous cropping soil is achieved, and the method has a certain promoting effect on the growth of crops of second stubble, increases crop yield and improves economic benefits.

The invention discloses an operation method for influence of long-term coal pile on soil bacteria based on multi-dimensional indexes, which comprises the following steps: (1) selecting research areasand collecting soil samples; (2) analysis of soil physical and chemical properties; (3) DNA extraction, PCR amplification and Illumina MiSeq sequencing; (4) processing of 16S and 18S sequencing data;(5) analysis of the influence of long-term coal piling on soil properties and microbial communities. Through the above steps, the influence of long-term coal piling in power plants on soil organic matter and soil bacterial diversity can be analyzed, which is of great significance for guiding environmental impact assessment and soil vegetation restoration of coal storage yards in coal-fired power plants.

The invention belongs to the technical field of soil fertilizers, and particularly relates to a special fertilizer for apples, and a fertilizing method thereof. The special fertilizer for apples has acore-shell structure, wherein the inner core is prepared from components including resin coated urea and ammonium polyphosphate, the shell is prepared from components including seaweed extract and acomposite microbial agent, the composite microbial agent is prepared from trichoderma and bacillus subtilis in a mass ratio of (0.5-2):(1-2), and the mass ratio of the resin coated urea to the ammonium polyphosphate to the seaweed extract to the composite microbial agent is (10-50):(1-20):(1-5):(0.1-1). The special fertilizer for apples has fertilizer particles with a shell-core structure, the number of soil bacteria can be increased, propagation of harmful fungi is inhibited, plant diseases and insect pests in soil are relieved, the yield, the single-fruit weight gain and the soluble sugar content are remarkably increased, and the quality of apples is improved; and the cost is greatly reduced while the utilization rate of the fertilizer is increased.