75 results about "Soil bacteria" patented technology

The invention relates to a liquid microbial fertilizer with nitrogen-fixing, phosphorus-dissolving and potassium-dissolving effects, and a preparation method thereof. The liquid microbial fertilizer consists of the following microbial fermentation liquids in parts by volume: 1 to 10 parts of azotobacter chroococcum, 1 to 30 parts of bacillus mucilaginosus, 1 to 10 parts of phosphorus-dissolving bacillus megatherium, 1 to 10 parts of bacillus amyloliquefaciens, 1 to 10 parts of bacillus subtilis, 1 to 5 parts of photosynthetic bacteria and 1 to 10 parts of streptomyces microflavus. The functional bacteria serve as main functional flora of the microbial fertilizer, so that the obtained product has remarkable effects of fixing nitrogen, dissolving phosphorus, dissolving potassium and resisting disease, can enhance the total metabolic activity of soil bacterial community and the diversity of the bacterial community, improve the soil micro-ecological environment, promote growth and reproduction of beneficial microorganisms and inhibit the growth of pathogenic bacteria, is an ideal fertilizer in green agriculture and organic agriculture, and has a wide prospect in agricultural sustainable development.

The invention discloses a method for killing soil bacteria and insects and improving soil and a special device for killing bacteria and insects, wherein the method for killing soil bacteria and insects and improving soil comprises the following steps: first, rotatably plowing the surface soil, where the rotary plowing depth is not less than 35 cm; second, shoveling the surface soil from the land, and lifting the shoveled surface soil and then baking the surface soil by flame; and third, complementing microorganism, compost and trace element; the special device comprises a box, wherein a shoveling plate is arranged in the front of the box body; a soil inlet is arranged on the front wall of the box body; a transmission shaft is arranged above the back of the shoveling plate; soil-lifting blades are distributed on the transmission shaft annularly; a first tilting plate is arranged on the middle lower part of the box body; a gas pipe is arranged at the top of the box body; and a flame stove is arranged on the box body. When the special device and the method disclosed by the invention are used for killing bacteria and insects, the cost is low, the insect-killing effect is good and the organic activity and microorganism activity in the soil are ensured.

A biodegradable, non-toxic firefighting concentrate composition. The preferred compositions include a mixture of a C14-C18 5 mole ethoxylated tallow amine, 2 mole ethoxylated coconut amine, and 5 mole ethoxylated coconut amine, wherein the blend is neutralized with a C6 carboxylic acid; an aliquot of 33 parts is added to 3 parts of a 2 mole ethoxylated linear alcohol having 6 to 16 carbon atoms, and enough water to bring the total weight percentage to 70%. Ten to fifteen parts of sodium lauryl sulfate is added and the balance of water is added to bring the total up to 100%. The composition is also effective when mixed with glycol dimethyl ether, the incorporation of a filming protein based agent, dye, or foam forming materials. In addition, the composition is useful with soil bacteria for remediating soil contaminated with hydrocarbon fuel and for facilitating fuel dispersion and degradation within bacterial-action sewage systems.

Mycorrhizal and rhizobial associations represent the two most important symbiotic relationships between higher plants and microorganisms, providing access to otherwise limiting supplies of phosphate and nitrogen, respectively. Although many higher plants are able to establish a symbiotic relationship with arbuscular mycorrhizal fungi, legumes are unusual among plants because they also form associations with nitrogen fixing soil bacteria called rhizobia. This symbiosis requires the production of bacterial signals, “Nod factors” that trigger several key developmental responses in the host plant (Dénarié et al., 1996). The DMI1 gene of the model legume M. truncatula plays a major role both in the early steps of Nod factor signaling and in the establishment of mycorrhizal symbiosis. Dmi1 mutants do not exhibit many of the early responses to Nod factors and are incapable of forming nitrogen fixing root nodules. Here we describe the cloning and preliminary characterization of DMI1. The DMI1 gene encodes a novel protein with low global similarity to ligand-gated cation channels of archaea. The protein is highly conserved in angiosperms and ancestral to land plants. Interestingly a putative A. thaliana DMI1 orthologous gene is expressed in roots. As A. thaliana is unable to establish a mycorrhizal symbiosis, this finding suggests that DMI1 may also exhibit a function that is independent of symbiotic interactions.

An agriculturally effective active ingredient is applied to plant foliage before, after, or simultaneously with an enhancer component containing a substantially pure bacterial culture, suspension, spores, or cells of a bacteria selected from the genus Bacillus or a soil bacteria.

The invention discloses a method and equipment for killing bacteria and pests in soil of a greenhouse. The prior method for killing soil bacteria and viruses as well as underground pests by chemical agents fails to achieve ideal sterilizing and disinsectizing effects. The method comprises the following steps: high-temperature gas produced by fuel (5) in a baking oven passes through a hot air pipe (7), a hot air hole (9) and a hot air chamber (8), and enters the soil through rake ditches formed by rake teeth (10) to kill the bacteria and the pests in the soil of the greenhouse. The method has the advantages of killing the bacteria in the soil of the greenhouse and having low pest cost as well as good sterilizing and disinsectizing effects. The equipment has the advantages of simple structure, small volume and convenient operation, and is suitable to be popularized and applied in the greenhouse. The method smartly utilizes the rake ditches formed by operating the rake teeth to ensure that the high-temperature gas evenly enters the soil to combine raking land with sterilization and disinsectization without drilling holes to the soil additionally, thereby saving labor and time, and having good effect at the same time.

The invention discloses a high-throughput absolute quantification method for soil bacteria. The high-throughput absolute quantification method includes steps of whirling and re-suspending E. coli HTAQ-GFP thalli in sterile water and regulating bacteria suspension in ultraviolet spectrophotometers OD <600nm=1.0>; 2), absolutely quantifying internal standard strains HTAQ-GFP and acquiring the absolute content of internal standard strains E. coli HTAQ-GFP in bacteria solution; 3), adding the bacteria solution with the internal standard strains HTAQ-GFP to soil samples and uniformly stirring and mixing the bacteria solution and the soil samples with one another; 4), extracting soil DNA (deoxyribonucleic acid) and carrying out high-throughput sequencing on amplicons of 16S rRNA [16S ribosomal RNA (ribonucleic acid)] genes to obtain classification composition information of bacterial community structures and corresponding relative abundance; 5), acquiring the total quantities of native bacteria in soil and the absolute contents of various classification units according to the absolute contents of the internal standard strains HTAQ-GFP and the relative abundance of Escherichia in high-throughput sequencing results.

The invention belongs to the field of biological bacterial fertilizers, in particular to a composite microbial agent. The composite microbial agent is prepared from five types of beneficial soil bacteria, wherein bacillus subtilis (TMQK07), bacillus laterosporus (TMQC01) and lactobacillus casei (TMQR02) are mixed according to a living bacteria count proportion of (0.9 to 1) to (0.8 to 0.9) to (5 to 6), and a live bacteria count is larger than or equal to 3,000,000,000/ml; and moreover, streptomyces griseus (TMQMH05) fermentation liquor containing 700 U/ml or above of streptomycin, and trichoderma harzianum (TMQMH12) fermentation liquor with a spore count of 500,000,000/ml are mixed with a mixture of the bacillus subtilis, the bacillus laterosporus and the lactobacillus casei, and polysaccharides, gamma-polyglutamic acid and humic acid are added as synergists in order to overcome the continuous cropping of crops, lower the incidence rate of wilt, and achieve the effects of resisting bacteria, tonifying plants, preventing diseases and improving yield.

The invention discloses primers for identifying pathogenic bacteria of tomato neck-root rot and wilt and a kit. The primer sequences for identifying pathogenic bacteria of tomato neck-root rot are shown as SEQ ID NO: 1 and SEQ ID NO: 2, and the primer sequences for identifying pathogenic bacteria of tomato wilt are shown as SEQ ID NO: 3 and SEQ ID NO: 4. The primers are strong in specificity and high in detection accuracy, can be used for detecting No. 1 physiological races of fusarium oxysporum tomato neck-root rot specialis and fusarium oxysporum tomato wilt specialis, and is significant for early quick detection of tomato neck-root rot and wilt, soil bacteria community distribution as well as prevention and control of diseases.

The present invention discloses a composite fertilizer special for tomatoes, which comprises the following raw materials in part by weight: 10-15 parts of ammonium molybdate, 20-25 parts of diammonium phosphate, 20-30 parts of potassium chloride, 1-4 parts of zinc borate, 5-10 parts of potash magnesium sulphate fertilizer, 1-2 parts of manganese sulfate, 1-3 parts of zinc-amino acid chelate, 1-2 p,arts of iron-amino acid chelate, 1-4 parts of copper-amino acid chelate, 2-3 parts of molybdenum-amino acid chelate, 30-40 parts of pond sludge, 15-20 parts of corn cakes, 20-25 parts of rape seed cakes, 30-40 parts of fully rotten byre manure, 30-40 parts of hog manure, 1-2 parts of borax, 10-15 parts of peat soil, 2-5 parts of attapulgite, 2-3 parts of nano-river sand powders, 2-5 parts of phosphorus rock powders, 0.5-0.8 part of naphthalene acetic acid, 0.05-0.1 part of gibberellin, 1-2 parts of saccharomycetes and 2-4 parts of soil bacteria. The composite fertilizer special for tomatoes is rich in nutrients, good in slow-release effect, green and environment-friendly, which can improve the soil.

The invention discloses a method for detecting the number of soil bacteria based on real-time fluorescent quantitative PCR, and belongs to the technical field of biology. The method comprises the following steps: 1) extracting soil sample total DNA and adjusting the total DNA to a preset concentration to prepare a to-be-detected soil sample template DNA solution; 2) extracting the total DNA of template escherichia coli, measuring the DNA concentration, and preparing a standard curve template DNA solution; 3) carrying out real-time fluorescent quantitative PCR amplification on the to-be-detected soil sample template DNA solution and the standard curve template DNA solution; 4) drawing a standard curve; and 5) calculating the number of bacteria per gram of dry soil. According to the method,the steps of adding an internal standard strain for fluoroscopy and the like are omitted, the operation difficulty is greatly reduced, connection, conversion and qPCR amplification are not needed, operation is easy, convenient and easy to learn, and the target fragment is good in specificity, high in data accuracy and good in reproducibility.

The invention relates to a comparative test method for the influence of organic fertilizers and inorganic fertilizers on the activity of soil in a paddy field. The comparative test method comprises the following steps of: test design, fertilizer application, disease and insect prevention and control, soil physical and chemical property determination and soil acquisition. The comparative test method has the benefits that the physical and chemical properties of the soil, the number of microorganisms in soil fauna and the soil enzyme activity are tested, the contents of nitrogen, phosphorus and potassium, the number of soil bacteria, actinomycetes and funguses and the number of various main enzymes in the soil in the organic and inorganic fertilizer environment are comprehensively detected, a comprehensive and effective test method is provided for people to determine the optimum proportioning of the organic fertilizers and the inorganic fertilizers, the guide is detailed, and the method is scientific.

The present invention discloses nutrient soil for radish planting. The nutrient soil is prepared from the following dry powder of raw materials in parts by weight: 50-70 parts of pond mud, 20-25 parts of corn cobs, 10-15 parts of longan cores, 5-10 parts of banana peel, 5-10 parts of camellia seed, 3-5 parts of rosa laevigata michx and 1-2 parts of ganoderma. The nutrient soil disclosed by the present invention is hign in fertilizer efficiency, and various nutrient elements are added into soil property. The nutrient soil can inhibit the growth of soil bacteria.

Stable, liquid fertilizer compositions comprising nickel, cobalt, and molybdenum are provided herein. The fertilizer compositions are capable supplying high levels of micronutrients to seeds at low doses of the compositions. The compositions generally have low salinity and a pH from about 6.0 to about 8.0, thereby avoiding the high mortality rates of beneficial soil bacteria, which has been a major problem with prior seed fertilizers. Also provided herein are methods of forming and using the fertilizer compositions. Notably, it has been discovered that monoethanolamine molybdate solutions can be used to provide high levels of molybdenum to seed surfaces in a stable liquid formulation.

The invention discloses a solid flower fertilizer formula and process. The formula ratio is 1-2 parts of potassium nitrate, 1.2-1.5 parts of calcium superphosphate, 0.5-0.9 parts of calcium nitrate, 0.8-1.5 parts of magnesium sulfate, and 0.01-0.1 parts of iron sulfate , 0.01-0.05 parts of manganese sulfate, 0.001-0.01 parts of borax, 0.001-0.005 parts of zinc sulfate, 0.001-0.005 parts of copper sulfate; can make full use of planting time, planting flowers are not limited by seasons, and can be carried out in any season; Invaded by bacteria in the soil, no pests and diseases occur, so there is no need to use pesticides to avoid pesticide pollution; flowers can grow luxuriantly without removing clutter and impurities; there will be no mildew and rotten smell caused by fertilization; dedicated to The cultivation of various flowers can also be used for the cultivation of various vegetables.

The invention discloses an operation method for influence of long-term coal pile on soil bacteria based on multi-dimensional indexes, which comprises the following steps: (1) selecting research areasand collecting soil samples; (2) analysis of soil physical and chemical properties; (3) DNA extraction, PCR amplification and Illumina MiSeq sequencing; (4) processing of 16S and 18S sequencing data;(5) analysis of the influence of long-term coal piling on soil properties and microbial communities. Through the above steps, the influence of long-term coal piling in power plants on soil organic matter and soil bacterial diversity can be analyzed, which is of great significance for guiding environmental impact assessment and soil vegetation restoration of coal storage yards in coal-fired power plants.