26 results about "Soil bacteria" patented technology

The invention discloses a salt-alkali-tolerant growth-promoting ectopseudomonas DHS-HJ-IAA-2 strain and an application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of salt-resistant, alkali-resistant and drought-resistant pseudomonas sp. DHS-HJ-IAA-2 is screened, and the pseudomonas sp. DHS-HJ-IAA-2 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36164. Experimental results show that the ectopseudomonas DHS-HJ-IAA-2 has the functions of producing IAA, fixing nitrogen, dissolving potassium, degrading cellulose and lignin, inhibiting phytopathogen and the like, and can remarkably improve the bacteria abundance of saline-alkali soil, improve soil fertility and promote plant growth in a saline-alkali environment. The invention provides a new strain resource and a technical means for improving saline-alkali soil and planting crops on the saline-alkali soil, and realizes improvement of the saline-alkali soil from two aspects of soil-crop-microorganism comprehensive action and microorganism-plant synergistic action.

The utility model discloses a soil flora culture device with a split type bottom double-spiral belt and a reverse screw, and relates to a stirring device. The barrel bottom and the barrel body are mounted in the base; the lower end of the barrel body is communicated with the barrel bottom; the connecting cover is fixedly connected with the upper end of the barrel body; the barrel cover is connected with the connecting cover through a connecting device; the driving mechanism is fixed in the base; the stirring mechanism is mounted in the barrel body; two ends of the stirring mechanism are respectively connected with the barrel cover and the driving mechanism. According to the utility model, through the mutual matching among the outer-ring helical ribbon, the inner-ring helical ribbon and the connecting rod, the mixing and stirring of soil in the vertical direction, the cutting and mixing and stirring in the horizontal direction, the scraping of the inner wall of the barrel body and the filling of soil in a central hole in the barrel body can be realized; the stirring mechanism not only can realize rotary stirring around the central shaft, but also has upward thrust of a stirring rod and downward pressure of a helical ribbon, soil with the humidity of about 45% can be uniformly stirred, even large soil can be uniformly stirred, the stirring effect is strong, and the efficiency is high.

The application of "a unit type tidal wetland restoration method based on a target" belongs to the technical field of wetland ecosystem restoration methods. The unit type tidal wetland restoration method based on a target comprises the following steps: (1) determining a target; (2) constructing an ecological unit on the tidal wetland to be restored through microtopographic modification; (3) applying biochar materials and salt-resistant ecological materials in the ecological unit; and (4) configuring waterfront plants in the ecological unit. The restoration method utilizes natural tidal fluctuation to adjust the water and salt conditions of the ecological unit (salt reduction of more than 80%) and to maintain the target water level (0-2 m) as needed, optimizes the soil microbial community structure (soil bacterial richness is increased by more than 200%), improves the plant germination rate and survival rate in the process of tidal restoration (the germination rate is more than 90%, and the survival rate is increased to more than 62%), and enriches the plant configuration varieties in the riparian zone (≥12).

The application discloses a rotary tillage mechanism with soil remediation function, and relates to the technical field of tillage equipment. The rotary tillage mechanism comprises a power system, a vehicle frame, a rotary tillage device and a high-voltage module. The rotary tillage device is arranged on the vehicle frame, and the power system drives the rotary tillage device to work. The high-voltage module and a plurality of dielectric barrier discharge devices are arranged below the vehicle frame. The dielectric barrier discharge devices intermittently ionize air to generate active substances and energy higher than corona discharge and spark discharge, so as to kill organisms in the soil. The high-voltage module provides a voltage of 5-8kV for the dielectric barrier discharge devices. The application can not only kill soil bacteria, but also effectively degrade residual pesticides in the soil, repair the soil structure and improve the growth environment of crop root systems, so as to ensure high yield of crops. The application is convenient for manual operation, saves time and effort, greatly improves production efficiency, reduces the labor intensity of farmers, and has the advantages of safety in use, no pollution to the environment and energy saving.

The invention belongs to the technical field of microorganisms, and particularly relates to ochrobactrum G3 and application thereof. The ochrobactrum G3 is classified and named as ochrobactrum and is preserved in the China Center for Type Culture Collection on November 17, 2025, and the preservation number of the ochrobactrum G3 is CCTCC NO: M 20252574. Soil bacteria are separated from rhizosphere soil of sorghum, antagonistic strains of the sorghum anthracnose are screened out, form and molecular identification of the strains is completed, the antibacterial spectrum of volatile organic compounds (VOCs) generated by the antagonistic strains and the control effect of fermentation bacteria liquid on sorghum are achieved, a new biocontrol resource is provided for prevention and treatment of the sorghum anthracnose, and the method has a wide application prospect. And a foundation is laid for further preventing and treating diseases by using the antagonistic strain.

The embodiment of the invention provides a soil bacterial community structure evaluation method and system based on an intermediate infrared spectrum transfer function and application, and relates to the technical field of soil microbial ecology. The method comprises the following steps: S1, collecting a soil sample; s2, acquiring a mid-infrared diffuse reflection spectrum of the soil sample to be detected; s3, acquiring an environment covariant corresponding to the soil sample, wherein the environment covariant comprises at least one of soil physicochemical properties, climate data and topographic parameters; s4, splicing the mid-infrared diffuse reflection spectrum obtained in the step S2 and the environment covariable obtained in the step S3 to form a fusion feature vector as an input feature set; and S5, inputting the input feature set into a pre-trained nonlinear machine learning regression model, and outputting the relative abundance and / or OTU richness of at least one bacteriophage in the soil sample. According to the method, a technical route of combining a mid-infrared (MIR) spectrum and a spectral transfer function (STF) is adopted and is used for predicting the soil bacterial community structure.

This invention relates to the field of agricultural microbiology, and in particular to a strain of *Bacillus erythropoietinus* KF-B6 and its applications, especially in the control of continuous cropping obstacles in apple orchards. The *Bacillus erythropoietinus* KF-B6, with preservation number CGMCC No. 32131, possesses a complex set of functions including rapid and high EPS (explosive peroxide) production, high chitinase production, production of water-soluble and volatile antibacterial substances, and decomposition of macromolecular organic matter. The application of this bacterium, its microbial agents, and compound microbial fertilizers can effectively increase the number of rhizosphere soil bacteria, reduce the number of pathogenic fungi, increase the ratio of bacteria to fungi, and promote the recovery of the soil microbial ecosystem in apple orchards with continuous cropping obstacles; increase the proportion of water-stable aggregates, increase soil organic matter content, and increase soil enzyme activity, thus improving the soil; improve the survival rate of saplings planted during the renewal of new varieties in old orchards with continuous cropping obstacles, promote root vitality and plant growth, and comprehensively alleviate and control continuous cropping obstacles in apple orchards.

The application discloses application of Enterobacter kobei in controlling and resisting evolution of soil bacteria antibiotic resistance. Enterobacter kobei LB1 is preserved in the China General Microbiological Culture Collection Center on May 15, 2026, and has a preservation number of CGMCC No. 38684 and a preservation address of No. 1, Xibaixili, Chaoyang District, Beijing, China Institute of Microbiology. Enterobacter kobei LB1 and extracellular metabolites have the ability of controlling and resisting evolution of soil bacteria antibiotic resistance. The application verifies the inhibiting effect of the resistance mutant strain of the control agent through a soil microcosm experiment, realizes effective intervention on the evolution of soil microbial resistance, and has the advantages of green ecology, safety, high efficiency, long-term control and the like, and provides a new technical way for environmental antibiotic resistance pollution treatment.

The invention provides high-humus coal gangue-based bionic peat as well as a preparation method and application thereof, the preparation method comprises the following steps: (1) inoculating a soil bacterial suspension into a lignin screening liquid culture medium for enrichment culture to obtain a high-temperature-resistant bacterial solution H1; and (2) transferring the high-temperature-resistant bacterial liquid H1 into a coal gangue and garden waste mixed substrate culture medium, adding Tween 80 for fermentation, and drying to obtain the high-humus coal gangue-based bionic peat, the fermentation time is 20 to 35 days. According to the preparation method disclosed by the invention, the coal gangue-based bionic peat with high humus content can be simply and quickly obtained, the high-humus coal gangue-based bionic peat can be used for repairing various soils, and resource utilization is realized while the problem that a large amount of coal gangue and garden wastes are accumulated is solved.

The invention belongs to the technical field of microorganisms, and particularly relates to a complex microbial inoculant for relieving phosphorus deficiency in the seedling stage of corn covered with straw and application of the complex microbial inoculant. Bacillus subtilis, saccharomycetes and pseudomonas fluorescens are compounded into the complex microbial inoculant, saccharomycetes secrete IAA and siderophores to promote the root development of seedlings, through domestication culture, the adaptability of the microbial inoculum in a straw-covered low-temperature environment is enhanced, the activation capacity on phosphorus is improved, the phosphorus nutrition level is synergistically improved in cooperation with bacillus subtilis, pseudomonas fluorescens can enhance the resistance of the seedlings to low-temperature stress, and the survival rate of the seedlings is increased. Soil-borne pathogenic bacteria which cannot be effectively inhibited by some soil florae are specifically inhibited, the influence of harmful bacteria on the activation effect of phosphorus is reduced, the three strains are matched to remarkably improve the content of rapidly available phosphorus in soil, reduce the incidence rate of phosphorus deficiency symptoms and improve the stress resistance and growth indexes in the corn seedling stage, and the compound microbial agent is suitable for a straw covering cultivation mode.

The invention discloses a vegetation-soil-microorganism synergistic remediation treatment method for a stony desertification slope, and relates to the technical field of soil remediation, and the method comprises the following steps: S1, precise evaluation of site conditions: uniformly arranging 10m * 10m quadrats on a target slope by adopting a GPS positioning and field sampling combined method, and measuring the slope, rock exposure rate, soil thickness and soil organic matter content (SOM); s2, vegetation three-dimensional configuration and field planting: selecting local stress-tolerant species, and constructing a three-dimensional community according to a plant / cluster number ratio of (2-4): (2-4): (3-5); according to the method, vegetation-soil-microorganisms are regarded as an organic whole, a synergistic mutual promotion mechanism of the vegetation, the soil and the microorganisms is systematically constructed, the vegetation fixes a carbon source through photosynthesis, root exudates nourish the microorganisms, the soil provides a physical carrier and a basic environment for the vegetation and the microorganisms, and the microorganisms supply energy to the vegetation through nutrient conversion; and a forward circulation of vegetation soil fixation, soil bacterium cultivation and microbe vegetation promotion is formed.

The application relates to the field of agricultural technology, and particularly discloses a disease soil bacteria detection and fertilizer application repair and improvement method and application thereof. The disease soil bacteria detection and fertilizer application repair and improvement method provided by the application comprises the following steps: (1) in October of the current year, the organic matter content in disease soil is detected, and the soil conditioner dosage is determined; in November, the soil conditioner is applied to the disease soil; (2) in early May of the next year, the relative abundance of fungal and bacterial genus level pathogenic bacteria in normal soil and disease soil is detected, and the microbial agent dosage is determined; in mid-May, the microbial agent is applied to the disease soil. The disease soil bacteria detection and fertilizer application repair and improvement method provided by the application dynamically adjusts the microbial agent dosage by detecting and analyzing the microbial structure difference between disease soil and normal soil in the crop growth process, so that the microbial system of the disease soil is improved, and the repair of the heavy-rotation and continuous-cropping obstacle soil is realized.

This invention proposes a method for reducing cadmium in paddy fields using common soil microorganisms, belonging to the field of agricultural technology. Addressing the problems of high cost, secondary pollution, and soil structure damage associated with existing physicochemical cadmium reduction methods, this invention provides a bioremediation technology using Bacillus subtilis ATCC6633 as the functional strain. This method involves activating laboratory-preserved Bacillus subtilis ATCC6633 bacterial powder to prepare a microbial agent, which is then directly applied to the surface water of paddy fields. Utilizing the specific adsorption and conversion of cadmium ions by the strain, effective passivation and removal of cadmium from paddy fields are achieved. This invention uses common soil microorganisms, exhibiting strong environmental compatibility and eliminating the need for chemical agents, fundamentally avoiding the secondary pollution problems caused by traditional chemical precipitation methods. It is both eco-friendly and economically feasible, significantly improving rice quality and providing an effective green remediation approach for cadmium pollution in paddy fields.

The invention discloses beneficial complex microbial inoculant powder, a special bio-organic fertilizer for early rice and a preparation method of the special bio-organic fertilizer, and relates to the technical field of bio-organic fertilizers. The invention relates to a microbial organic fertilizer, which is prepared from the following components in percentage by mass: 40 to 50 percent of iron oxidizing bacteria (Sideroxydans), 15 to 25 percent of ammonia oxidizing bacteria (MND1), 15 to 25 percent of thiobacillus (Thiobacillus) and 5 to 10 percent of pseudomonas (Pseudomonas), and can be used for promoting the yield increase of crops, fertile soil and acid reduction and improving the ecological function of a farmland. The invention further discloses application of the beneficial complex microbial inoculant powder to preparation of the special bio-organic fertilizer for early rice. The prepared special bio-organic fertilizer for early rice can obviously improve the fertilizer retention and supply properties of soil and the pH value of the soil; the abundance of soil bacterial microflora is improved, the microbial diversity indexes of bacteria Chao1, Shannon and the like are increased, and the nutrient circulation rate is accelerated; soluble organic carbon, microbial biomass carbon and nitrogen cycle related enzyme activity of soil are enhanced, and high rice yield and soil quality index are maintained.

This invention belongs to the field of plant rhizosphere research and discloses a method for determining the regulatory effect of exogenous selenium application on the rhizosphere microecology of Amorphophallus bulbifera. The method includes: experimental design and sample collection; soil microbial DNA extraction and amplicon sequencing; and quality control and analysis of the microbiome data. This invention utilizes Illumina MiSeq high-throughput sequencing technology to analyze the changes in the composition and diversity of rhizosphere soil bacteria communities in Amorphophallus bulbifera under exogenous selenium treatment, and further analyzes the functions of related communities through PICRUSt functional prediction analysis. The conclusions are as follows: exogenous selenium treatment induces significant changes in the rhizosphere soil bacterial community of Amorphophallus bulbifera, which is beneficial for creating a stable and more diverse rhizosphere microbial community structure.

This invention relates to the field of biopesticide technology, and particularly to a compound formulation for controlling pineapple root-knot nematodes, its preparation method, and its application. The aforementioned compound formulation for controlling pineapple root-knot nematodes comprises, by weight percentage: 0.1-5% azadirachtin or compound azadirachtin, 30-60% castor seed meal, 35-65% activated carbon, and the remainder being a binder. This invention uses a compound of activated carbon, azadirachtin, and castor seed meal. Azadirachtin achieves highly effective contact killing by inhibiting the nematode's respiratory chain and nerve signal transmission, without developing resistance. Activated carbon acts as a slow-release carrier, prolonging the efficacy (lasting for more than 90 days) and adsorbing soil toxins to improve the microenvironment. Castor seed meal provides organic matter to promote the proliferation of beneficial soil bacteria and improve soil structure; it is also rich in ricin, effectively inhibiting nematode egg hatching. The combined use of these three ingredients achieves synergistic effects through a triple mechanism of slow release, killing, and soil improvement.

The invention relates to the technical field of irrigation equipment, and particularly discloses a shallow-buried intelligent water-saving sprinkling irrigation unit which comprises an irrigation mechanism, the irrigation mechanism comprises a vertical pipe, the upper end of the vertical pipe is communicated with a quantum water distribution tank, and the two long side edges of the quantum water distribution tank are communicated with a middle side extending pipe and two symmetrically-arranged two-end side extending pipes respectively. The middle side extending pipe is provided with a middle adjusting valve, the two end side extending pipes are each provided with a two-side adjusting valve, the two short side ends of the quantum water distribution box communicate with two-end adjusting pipes which are symmetrically arranged correspondingly, the two-end adjusting pipes are each provided with a two-end adjusting valve, and the soil optimization mechanism comprises a quantum cast ring; the quantum cast ring is arranged in the quantum water distribution box; quantum cast rings are arranged in the quantum water distribution box, the quantum cast rings are used for exciting fluctuation of water molecules, eliminating harmful substances in soil and killing harmful bacteria in the soil, and carbon ash water can excite plant activity, cultivate soil effective microbial communities, repair and improve the soil and promote rooting and yield increase of the plants.

This invention discloses the application of isocitric acid in the control of bacterial wilt in plants, wherein the isocitric acid is derived from tomato root exudates. The inhibitory effect of isocitric acid on *Ralstonia solanacearum* was evaluated through shake-flask culture experiments and plate inhibition zone experiments, and the control effect of isocitric acid on bacterial wilt was determined using greenhouse pot experiments. The results showed that within the concentration range of 10–200 μM, isocitric acid significantly inhibited the growth of *Ralstonia solanacearum*, forming a distinct inhibition zone. Under greenhouse pot conditions, applying 0.2–0.5 mg / kg of isocitric acid to the soil effectively reduced the incidence of bacterial wilt in tomatoes, with a disease control rate of 21.43%–52.38%. Furthermore, isocitric acid can indirectly inhibit the occurrence of bacterial wilt by influencing the soil bacterial community. The isocitric acid used in this invention is derived from natural plant root exudates, possessing advantages such as environmental friendliness and high safety, and can be used as a novel inhibitor of tomato bacterial wilt, showing promising application prospects in the field of green control of plant diseases.

The invention relates to the technical field of mine ecological restoration, and discloses a mine ecological restoration method and system. The restoration method comprises the following steps: selecting an area with underground coal fire waste heat on a mine slope with residual coal spontaneous combustion as a restoration area; engineering reinforcement and slope surface fixation are conducted on the slope of the restoration area; a sludge base material with the water content being 60%-80% is laid on the fixed slope surface, and in-situ biological drying is conducted through underground coal fire waste heat till the volume reduction reaches a preset threshold value of 40%-60%; then, sequentially covering the dried sludge base material with a soil base material layer and a soil bacterium base material layer to construct a composite growth substrate; and finally carrying out vegetation maintenance on the slope surface. According to the method, the waste heat of the abandoned mine and municipal sludge treatment are ingeniously combined, harmlessness, reduction and recycling of the sludge are achieved, meanwhile, the problems that the soil temperature of a mining area is too high, and plants are difficult to survive are solved, and the ecological restoration purposes of treating waste with waste and achieving synergistic interaction are achieved.

The present application relates to the field of soil microbial analysis, in particular to a method for analyzing the rhizosphere microbial community structure of cultivated Fritillaria delavayi Hsiao and application thereof. The method comprises the following steps: step one, sample collection and processing; step two, determination of soil physicochemical properties; step three, gene sequencing: 16S amplification sequencing and high-throughput sequencing; step four, data analysis: conducting microbial community diversity analysis, community composition analysis, differential group analysis, community structure difference analysis, and correlation analysis of soil physicochemical properties and microbial community. The method can determine the dominant bacterial groups in the rhizosphere soil of Fritillaria delavayi Hsiao and the regulation factors of the dominant bacterial groups by physicochemical properties, provide a theoretical basis for the standardized planting of Fritillaria delavayi Hsiao and the screening of geographical provenances with excellent microbial interaction ability, and improve the yield and medicinal ingredients of cultivated Fritillaria delavayi Hsiao.

This invention relates to a microbial composition for controlling root-knot nematode disease in traditional Chinese medicinal materials and its application, belonging to the field of biotechnology. The composition comprises the following components: *Beauveria bassiana* (CGMCC No. 21047), lecithin, xanthan gum, wood vinegar, soybean peptides, potassium humate, and trace elements. Through the synergistic effect of specific functional microbial communities, the composition significantly promotes the growth of the underground parts of traditional Chinese medicinal materials, increases root biomass and root vigor, and enhances the plant's efficiency in nutrient absorption and utilization, particularly nitrogen utilization. Simultaneously, this preparation effectively controls root-knot nematode disease, significantly reducing the number of root-knot nematode infestation sources in the soil and the severity of root disease, achieving a high natural nematode reduction rate. Furthermore, this preparation activates the plant's own defense system, induces the synthesis of disease-resistant substances, and significantly enhances the activity of various defense enzymes. It also significantly improves the soil microecological environment, enhances the diversity and richness of the soil bacterial community, and is suitable for various crop planting systems.

This invention relates to a robust Bacillus subtilis strain ZKSW001, its inoculum, and its applications, belonging to the technical field of microbiology. The robust Bacillus subtilis ZKSW001 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36076, deposited on September 25, 2025, at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. This invention also provides the application of robust Bacillus subtilis ZKSW001 in inhibiting harmful soil bacteria, controlling root-knot nematodes, and promoting plant growth. The robust Bacillus subtilis ZKSW001 of this invention can promote crop growth, reduce fertilizer use and increase efficiency, control soil-borne fungal and bacterial diseases, and inhibit the growth of root-knot nematodes. It can reduce the use of chemical pesticides, lower pesticide residues, and promote the sustainable development of the agricultural economy.

The application belongs to the technical field of biodegradable polymer materials, and relates to a controllable biodegradable high-strength polylactic acid composite mulching film and a preparation method thereof. The mulching film is a three-layer composite structure, and the layers from top to bottom are a surface layer, a middle layer and an inner layer. The raw materials of the surface layer include PLA, PPC, PCL and a core-shell structure degradation regulator. The core-shell structure degradation regulator takes an imidazole phosphate as a core and takes calcium pyrophosphate as a shell. The raw materials of the middle layer include PLA, PBAT, an epoxy-containing compatibilizer and an epoxy-containing chain extender. The raw materials of the inner layer include PLA, starch and humic acid. The core-shell structure degradation regulator has a slow-release catalytic function and has a regulating effect on the degradation of the mulching film. The surface layer of the composite mulching film provides a degradation regulating function. The middle layer provides mechanical support. The inner layer activates soil bacteria and promotes crop growth. The three layers realize the contradictory requirements of high initial strength, long functional period and rapid degradation in the later period through synergistic action.

The application discloses a kind of full solid waste base biological fiber cooperates microorganism mineralization flow state solidified soil and preparation method thereof.The application is immersed in alkaline solution by biological base fiber, and modified carrier material is obtained;Waste concrete or waste brick ball is crushed and ball milled, and recycled micro-powder cementitious material is prepared;Modified carrier material is mixed with engineering waste, and recycled micro-powder cementitious material is dry mixed, and urease-producing bacterial solution or calcium carbonate precipitation-inducing microbial solution is added to stirring and standing, and mixture is obtained;Urea and calcium source are contained in the mixture and added to cementing fluid, and stirring is obtained, and the full solid waste base biological fiber flow state solidified soil is obtained.The application utilizes the alkaline excitation of recycled micro-powder, the carrier anchoring of biological fiber and the suspension synergistic effect of biological glue, effectively solves the problems of traditional MICP flow state soil bacteria easy loss, cement-free system easy segregation and large brittleness, and the prepared filler has the characteristics of high flowability, high toughness and low carbon environmental protection.

The invention relates to a microbial composition for preventing and treating root knot nematode disease of traditional Chinese medicinal materials and application thereof, and belongs to the technical field of biology. The composition is prepared from the following components: beauveria bassiana with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.21047, lecithin, xanthan gum, pyroligneous liquor, soybean peptide, potassium fulvate and medium trace elements. Through the synergistic effect of specific functional flora, the composition significantly promotes the growth of underground parts of traditional Chinese medicinal materials, improves the root biomass and root activity, enhances the nutrient absorption and utilization efficiency of plants, and especially improves the nitrogen utilization rate. Meanwhile, the preparation can effectively prevent and control the root-knot nematode disease, the number of root-knot nematode infection sources in soil and the severity of root diseases are remarkably reduced, and the high nematode natural decline rate is achieved. In addition, the preparation can activate the defensive system of plants, induce synthesis of disease-resistant substances and remarkably enhance the activity of multiple defensive enzymes. The soil micro-ecological environment is obviously improved, the diversity and richness of soil bacterial communities are improved, and the method is suitable for various crop planting systems.