Composite microbial agent with effects of reducing continuous cropping and wilt of crops and preparation method of composite microbial agent

A compound microbial agent and Fusarium wilt technology, applied in botany equipment and methods, biocides, plant growth regulators, etc., can solve the problems of high degree of harm, damage to the original structure of the soil, and high difficulty of prevention, and achieve improvement Effects of soil environment, increasing agricultural security, and reducing inputs

Inactive Publication Date: 2019-03-22
山东土木启生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] The reason is that the crops themselves have not been effectively decomposed due to the accumulation of some secretions left over from perennial planting, which inhibits the growth and high yield of the crops themselves. A large number of microorganisms die, and some soil-borne diseases continue to increase. Because of their strong concealment and high degree of damage, it is difficult to prevent soil-borne diseases, w

Method used

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  • Composite microbial agent with effects of reducing continuous cropping and wilt of crops and preparation method of composite microbial agent

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preparation example Construction

[0035] Another aspect of the present invention provides a method for preparing a compound microbial agent capable of reducing crop repeated cropping and blight, comprising the following steps: mixing five kinds of soil beneficial bacteria liquids in proportion, then mixing three kinds of biosynergists in proportion, and finally Top up with sterile water.

[0036]In this example, the preparation method of Bacillus subtilis (TMQK07): 20 g / L of sucrose, 5 g / L of ammonium sulfate, 45 g / L of bran, 2.0 g / L of sodium citrate, 0.4 g / L of dipotassium hydrogen phosphate trihydrate g / L, Magnesium Sulfate Heptahydrate 0.5 g / L, Ferric Sulfate Heptahydrate 0.03 g / L, pH value is natural, according to inoculum size 7%, ferment at 37°C for 24-38 hours, the number of viable bacteria reaches 20 billion / ml, The spore formation rate is above 98%.

[0037] In this example, the preparation method of Bacillus lateralis (TMQC01) is as follows: glucose 20 g / L, peptone 48 g / L, calcium chloride 0.45 g / L...

Embodiment 1

[0051] Embodiment 1: Application example

[0052] A compound microbial agent capable of reducing crop replanting effect and Fusarium wilt includes: a strain of Bacillus subtilis (TMQK07), a strain of Bacillus sporogenes (TMQC01), a strain of Lactobacillus casei (TMQR02), a strain of Streptomyces griseus (TMQMH05), a strain of Trichoderma harzianum (TMQMH12) and polysaccharides, γ-polyglutamic acid, humic acid, and four biosynergists.

[0053] The number of live bacteria in the fermentation broth of Bacillus subtilis (TMQK07) is 21.8 billion / ml, and the proportion is 95kg.

[0054] The number of live bacteria in the fermentation broth of Bacillus lateralis (TMQC01) is 20.9 billion / ml, and the ratio is 90kg

[0055] The number of live bacteria in the fermentation broth of Lactobacillus casei (TMQR01) is 80 million / ml, and the proportion is 500kg.

[0056] The streptomycin output of the Streptomyces griseus (TMQMH05) fermentation broth is 720U / ml, and the proportion is 65kg.

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Abstract

The invention belongs to the field of biological bacterial fertilizers, in particular to a composite microbial agent. The composite microbial agent is prepared from five types of beneficial soil bacteria, wherein bacillus subtilis (TMQK07), bacillus laterosporus (TMQC01) and lactobacillus casei (TMQR02) are mixed according to a living bacteria count proportion of (0.9 to 1) to (0.8 to 0.9) to (5 to 6), and a live bacteria count is larger than or equal to 3,000,000,000/ml; and moreover, streptomyces griseus (TMQMH05) fermentation liquor containing 700 U/ml or above of streptomycin, and trichoderma harzianum (TMQMH12) fermentation liquor with a spore count of 500,000,000/ml are mixed with a mixture of the bacillus subtilis, the bacillus laterosporus and the lactobacillus casei, and polysaccharides, gamma-polyglutamic acid and humic acid are added as synergists in order to overcome the continuous cropping of crops, lower the incidence rate of wilt, and achieve the effects of resisting bacteria, tonifying plants, preventing diseases and improving yield.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbial inoculum, and in particular relates to a compound microbial inoculum capable of reducing repeated cropping and blight of crops and a preparation method thereof. Background technique [0002] With the increasing desertification and land pollution in the world, there is less and less land suitable for planting crops. Under the huge population pressure in our country, the contradiction between people and land is getting bigger and bigger. Increasing the multiple cropping index (repeated cropping) is the solution to the problem the only way. However, several years of heavy cropping have caused a large number of soil-borne diseases, resulting in a large area of ​​crop production reduction and extinction. In particular, some medicinal materials with a high effect of heavy cropping, such as life in Changbai Mountain, Panax notoginseng in Southwest China, etc. have no land to grow. Banana ...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N63/04A01N43/16A01N37/44A01N61/00A01P3/00A01P1/00A01P21/00
CPCA01N37/44A01N43/16A01N61/00A01N61/02A01N63/00A01N63/30
Inventor 郑安科徐骠张俊庆
Owner 山东土木启生物科技有限公司
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