Composite microbial agent with effects of reducing continuous cropping and wilt of crops and preparation method of composite microbial agent
A compound microbial agent and Fusarium wilt technology, applied in botany equipment and methods, biocides, plant growth regulators, etc., can solve the problems of high degree of harm, damage to the original structure of the soil, and high difficulty of prevention, and achieve improvement Effects of soil environment, increasing agricultural security, and reducing inputs
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[0035] Another aspect of the present invention provides a method for preparing a compound microbial agent capable of reducing crop repeated cropping and blight, comprising the following steps: mixing five kinds of soil beneficial bacteria liquids in proportion, then mixing three kinds of biosynergists in proportion, and finally Top up with sterile water.
[0036]In this example, the preparation method of Bacillus subtilis (TMQK07): 20 g / L of sucrose, 5 g / L of ammonium sulfate, 45 g / L of bran, 2.0 g / L of sodium citrate, 0.4 g / L of dipotassium hydrogen phosphate trihydrate g / L, Magnesium Sulfate Heptahydrate 0.5 g / L, Ferric Sulfate Heptahydrate 0.03 g / L, pH value is natural, according to inoculum size 7%, ferment at 37°C for 24-38 hours, the number of viable bacteria reaches 20 billion / ml, The spore formation rate is above 98%.
[0037] In this example, the preparation method of Bacillus lateralis (TMQC01) is as follows: glucose 20 g / L, peptone 48 g / L, calcium chloride 0.45 g / L...
Embodiment 1
[0051] Embodiment 1: Application example
[0052] A compound microbial agent capable of reducing crop replanting effect and Fusarium wilt includes: a strain of Bacillus subtilis (TMQK07), a strain of Bacillus sporogenes (TMQC01), a strain of Lactobacillus casei (TMQR02), a strain of Streptomyces griseus (TMQMH05), a strain of Trichoderma harzianum (TMQMH12) and polysaccharides, γ-polyglutamic acid, humic acid, and four biosynergists.
[0053] The number of live bacteria in the fermentation broth of Bacillus subtilis (TMQK07) is 21.8 billion / ml, and the proportion is 95kg.
[0054] The number of live bacteria in the fermentation broth of Bacillus lateralis (TMQC01) is 20.9 billion / ml, and the ratio is 90kg
[0055] The number of live bacteria in the fermentation broth of Lactobacillus casei (TMQR01) is 80 million / ml, and the proportion is 500kg.
[0056] The streptomycin output of the Streptomyces griseus (TMQMH05) fermentation broth is 720U / ml, and the proportion is 65kg.
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