13 results about "Streptomyces griseus" patented technology

The invention discloses a culture medium suitable for producing melanin by bacteria. The culture medium comprises 5-15 g / L of soluble starch, 10-20 g / L of peptone, 5 g / L of sodium chloride, 5-12.5 mg / L of copper sulfate and 1 g / L of L-tyrosine. The invention also discloses an application of the culture medium suitable for producing melanin from bacteria in high-yield melanin. The streptomyces griseus disclosed by the invention can be used for producing the melanin, and the yield of the melanin reaches 7.74 g / L by optimizing a culture medium formula and culture conditions; furthermore, the conditions of the fermentation culture medium are optimized, the production period is shortened, and the production cost is reduced.

The invention discloses a microbial agent for improving disease resistance of tea trees and a preparation method of the microbial agent, and belongs to the field of agricultural disease prevention and control. According to the invention, the optimal strain compounding condition is obtained by screening the strain compounding condition, and experiments prove that the fungicide prepared when the brevibacterium Hainan powder ACCC 60371 and the streptomyces griseus var rust CICC 23624 are compounded according to the mass ratio of 2: 5 can enable the prevention and treatment effects of tea leaf blight and zonate spot to reach 88.58% and 84.41% respectively, so that the fungicide can be used for preventing and treating the tea leaf blight and zonate spot respectively. And the polyphenol content is obviously superior to the effect of preventing and treating the two tea tree diseases by single bacteria. The invention provides a strain source and a method for improving disease resistance and tea quality.

The invention provides a preparation method of loropetalum chinense extract and application of the loropetalum chinense extract in preparation of products for wound healing, and relates to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: mixing a loropetalum chinense raw material, water and composite biological bacteria, and carrying out enzymolysis to obtain an enzymolysis product; the composite biological bacterium is a mixture of phanerochaete chrysosporium and streptomyces griseus; carrying out gradient alcohol extraction on the enzymolysis product, merging alcohol extracts, extracting with petroleum ether, and concentrating an organic phase to obtain a component 1; the component 1 is adsorbed by macroporous adsorption resin, the resin is washed with deionized water and then eluted with an eluent, 5-35 min eluent is collected, combined and dried, and the component 1 is obtained. The elution phase is a mixture of cyclohexane, ethyl acetate and acetonitrile. The loropetalum chinense extract is prepared through a specific preparation method, and the wound healing effect is remarkably improved.

The invention relates to application of trichoderma harzianum to prevention and treatment of potato black scurf and scab. The trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.40017 can well inhibit rhizoctonia solani, so that the effect of preventing and treating potato black scurf is achieved; trichoderma harzianum with the preservation number of CGMCC No.40017 can also inhibit streptomycete, such as streptomyces scabies, streptomyces scabies and streptomyces griseus, so that the effect of preventing and treating potato scab is achieved, the strain or the microbial agent containing the strain is applied to the planting process of potatoes, potato black scurf and scab can be effectively prevented and treated, and the effect of preventing and treating potato scab is achieved. The method has important significance in agricultural production.

Disclosed are novel bioremediation formulations and inocula for formulation of bioremediation formulations. The formulations of the present disclosure comprise viable microorganisms of the species Paenibacillus xylanexedens, Pseudomonas fluorescens, Bacillus safensis, Streptomyces griseus, Micrococcus luteus, and Bacillus megaterium and can be used to degrade glyphosate residue associated with a substrate contaminated with glyphosate, and thereby remedially treat the substrate. Related methods are also disclosed.

The invention relates to the technical field of agricultural biology, and discloses a preparation method and application of a microbial fertilizer. Bacillus-streptomyces griseus quorum sensing is synergistically induced through AHL and GBL, so that the activity of nitrogenase and growth-promoting metabolite is remarkably improved; sodium alginate-chitosan / polyvinyl alcohol microcapsules are adopted for embedding, and the survival rate at the normal temperature for 30 days is larger than or equal to 90%; and further granulating with an organic carrier and a slow-release agent to realize continuous bacterium release for 30 days. Field trials show that the yield of crops such as vegetables is increased by 10-25% under the condition that the chemical fertilizer is reduced by 20-30%. The method is simple in process, low in cost and suitable for multiple crops and multiple types of soil, and has a good industrialization prospect.

The invention discloses streptomyces griseoflavus 25-6 and an application thereof. The streptomyces griseoflavus 25-6 has a better antagonistic effect on pathogenic fusarium pseudograminearum of wheat stem rot, and meanwhile, sterile fermentation liquor of the strain has an inhibiting effect on mycelial growth and spore germination of the fusarium pseudograminearum. Pot experiments show that the strain 25-6 fermentation liquor can reduce the occurrence of wheat basal stem rot, and the control effect is 60.3%. The strain 25-6 provided by the invention can provide a strain resource and a theoretical basis for biological control of wheat stem rot.

The invention discloses a method for preparing a special nutritional type improved microbial inoculum for planting rice in saline-alkali soil. The special nutritional type improved microbial inoculum comprises the following components in percentage by weight: 0.5-0.8% of white spirit yeast liquid (the bacterium content is 5 * 10 < 8 >-5 * 10 < 10 >); 0.5-0.8% of a bacillus subtilis solution (the bacterium content is 5 * 10 < 8 >-5 * 10 < 10 >); 0.5-0.8% of streptomyces griseus coccus liquid (the bacterium content is 5 * 10 < 8 >-5 * 10 < 10 >); 0.5-0.8% of indigenous microorganism bacterium liquid (the bacterium content is 5 * 10 < 8 >-5 * 10 < 10 >) of saline-alkali soil rice planting soil; 85%-95% of rice straw; and the balance water. According to the preparation method of the nutritional type improved microbial inoculum special for saline-alkali soil rice planting, the preparation cost is low, the productivity is high, the period of validity is long, the stability is high, the storage condition is simple, the transportation is convenient, different strains can be compounded and combined into a whole, the saline-alkali resistant span is large, and the environmental tolerance elasticity is high; the problem of weak saline-alkaline resistance is fundamentally solved.

The invention discloses a bio-organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizers. The preparation method comprises the following steps: respectively pretreating apple pomace, straw, cow dung and sheep manure, and mixing to obtain a mixture; bacillus subtilis, Streptomyces microflavus, Streptomyces griseus and Trichoderma harzianum are respectively cultured and then are mixed to obtain a mixed bacterial liquid. The preparation method comprises the following steps: adding a mixed bacterial solution into a mixture for high-temperature aerobic fermentation, then adding chitin for medium-temperature facultative fermentation, and finally adding selenized potassium humate and ground phosphate rock for anaerobic fermentation, thereby finally obtaining the biological organic fertilizer. The biological organic fertilizer with the characteristics of high organic matter content, high antibacterial activity and slow release of nutrients is produced within 30 days by virtue of pretreatment optimization of the raw materials, time sequence regulation and control of the complex microbial inoculants and accurate addition of synergistic substances, and the targets of waste recycling and ecological prevention and control of soil diseases are synchronously achieved.

This invention discloses a compound microbial fertilizer for improving soil acidification and its preparation method, relating to the field of soil improvement technology. It comprises the following components: microbial inoculant, modified nano-selenium complex, quicklime, biochar, humic acid, decomposed organic matter, and trehalose. The microbial inoculant contains *Bacillus belye*, *Lactobacillus plantarum*, and *Streptomyces griseus*. The modified nano-selenium complex is obtained by loading nano-selenium particles onto chitosan modified with ferulic acid. The fertilizer prepared by this invention achieves synergistic effects of soil improvement and increased rice yield through a triple mechanism of chemical neutralization, biological acid stabilization, and nano-inactivation, and also possesses selenium-enriching properties, demonstrating significant market potential.

The invention discloses a tomato bacterial wilt prevention and control method, which belongs to the technical field of tomato disease prevention and control, and comprises the following steps: S1, preparing a complex microbial inoculant: in the complex microbial inoculant, the volume ratio of bacillus velezensis bacterial liquid to stenotrophomonas rhizophila bacterial liquid to streptomyces griseorubens bacterial liquid is 1: (3-6): (2-4); the effective viable count of the bacillus riissii bacterial liquid, the stenotrophomonas rhizophila bacterial liquid and the streptomyces griseorubens bacterial liquid is not less than 1 * 10 < 8 > CFU / ml; s2, applying the complex microbial inoculant obtained in the step S1 to rhizosphere of tomato plants. The method provided by the invention can be used for effectively preventing and treating the tomato bacterial wilt caused by the Ralstonia solanacearum (Ralstonia solanacearum).

The present invention addresses the problem of providing a method for efficiently producing a specific peptide from a protein material containing therein an amino acid sequence composed of GTW. Provided is a method for producing a composition containing a GTW peptide, the production method including a step for allowing a protein material containing therein an amino acid sequence composed of GTW to interact with a protease, wherein the production method is characterized in that the pH at the start of interaction in the aforementioned step is 5.5 or higher, or in that the protease is derived from Aspergillus melleus, Streptomyces griseus, or Bacillus licheniformis, or in that a protein material containing the amino acid sequence composed of GTW in a portion thereof is a whey protein, or in that the whey protein is β-lactoglobulin.

The invention belongs to the technical field of microbial deodorants, and particularly relates to a composite microbial deodorant and a preparation method thereof. The preparation method comprises the following steps: preparing a bacillus subtilis seed solution, preparing a streptococcus thermophilus seed solution, preparing a streptomyces griseus seed solution, performing primary fermentation culture, modifying sepiolite and performing secondary fermentation culture. The sepiolite modification step comprises the following steps: putting sepiolite into a sodium hydroxide solution, raising the temperature to 60-64 DEG C, carrying out heat preservation stirring for 2.8-3.2 hours, after the heat preservation stirring is finished, putting the sepiolite into an ethanol solution, adding sodium dodecyl benzene sulfonate and polyethylene glycol 1000, carrying out heat preservation stirring at 38-42 DEG C for 26-33 minutes, after the heat preservation stirring is finished, adding kH550, raising the temperature to 58-62 DEG C, and carrying out stirring reaction for 1.8-2.2 hours; adding glutamic acid and chitosan oligosaccharide, and continuously stirring to react for 1.8-2.2 hours, so as to obtain modified sepiolite; the deodorant prepared by the invention is good in deodorization performance, good in long-term effect and excellent in stability.