155 results about "Streptomyces griseus" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

The invention belongs to the technical field of biological pesticides, discloses a Streptomyces l avendulae xjy strain which has been collected in the general microorganism center of CCCCM in 8.16.2007 with the collection number: CCMCC No.2130. The invention also discloses a separation and authentication method for the strain, a preparation method for antibiotic active substances of the Streptomyces l avendulae xjy strain, authentication for analysis structures of the active substances, prevention and cure experiments for metabolic active substances of the xjy strain against leaf muld of tomato, powdery mildew of cucumber and phytophthora blight of pepper, and growth-promoting experiments for the growth of the strain. The antibiotic active substances secreted by Streptomyces xjy strain is mainly nucleosides antibiotic having the characteristics of persistent effect and no medicinal hazard as well as having the effects of obviously preventing and curing vegetable diseases and promoting the growth to a certain extent.

The invention relates to compound microbial decomposed inoculas, and a preparation method and an application thereof. Effective components of the involved compound microbial decomposing inocula comprise bacillus amyloliquefaciens Ba168, paenibacillus peoriae BC-39, trichoderma harzianum, candida krusei, aspergillus niger and streptomyces griseus. The preparation method comprises that each strain are fermented separately, and the strains are mixed for use according to the proportion after the completion of the fermentation. The involved application is an application of the compound microbial decomposing inocula in degradation of crop straw. The compound microbial decomposing inocula not only give full play to unique degradation function of the bacillus on the straw, are combined with microbial compound flora for efficient synergy in the straw degradation process, thereby efficiently and rapidly degrading the straw, and significantly enhancing soil organic matter and humus levels.

The present invention discloses a composite microbial deodorant and a preparation method thereof. The composite microbial deodorant contains Rhodop seudanonas palustris, Candida utilis, Lactobacillus acidophilus, Streptomyces griseus and Bacillus subtilis, wherein the Rhodop seudanonas palustris, the Candida utilis, and the Lactobacillus acidophilus are subjected to mixed fermentation in a light-transmitting temperature-controllable fermentation tank, the Streptomyces griseus and the Bacillus subtilis are subjected to mixed fermentation in an aerating sterile air temperature-controlled fermentation tank, and the two fermented bacterial agents are mixed and added with essence, citric acid and glacial acetic acid to prepare the composite microbial deodorant. The composite microbial deodorant of the present invention has characteristics of simple preparation process and no secondary pollution, and provides good odor removing effects for toilets, farms and other occasions.

This invention relates to a method for preparing fermentation culture medium and producing biological pesticide from fermentation wastewater. The method comprises: (1) utilizing fermentation liquid, ethanol wastewater and monosodium glutamate wastewater as the raw materials, and Streptomyces griseus as the strain; (2) fermenting at 28 deg.C and 100-150 rpm for 7-10 days; (3) filtering, concentrating and purifying to obtain biological pesticide. The method utilizes fermentation wastewater to prepare fermentation culture medium and produce biological pesticide, thus can effectively combine the treatment of fermentation wastewater and utilization of useful resources in fermentation wastewater. The treatment rate is high and the cost is low. Besides, the method for produing biological pesticide has such advantages as simple process, low cost, high productivity, and low probability of culture medium pollution and good antibacterial effect of the biological pesticide.

The invention discloses a compound microbial agent for anaerobic digestion treatment of sludge and a production method of the compound microbial agent. The compound microbial agent is a liquid microbial agent which is prepared through fermentation culture and comprises the following bacteria: bacillus subtilis, streptomyces albus, streptomyces griseus, candida utilis, aspergillus sojae, lactobacillus bulgaricus, lactococcus lactis, rhodopseudomonas palustris, rhodopseudomonas capsulate and rhodobacter sphaeroides, and the number of total viable bacteria in 1ml of the compound microbial agent is not less than 2*10<8>. All the bacteria used by the compound microbial agent have metabiosis and symbiosis effects and form a biodegradation system with very high enzymatic activities. When the compound microbial agent disclosed by the invention is used for carrying out anaerobic digestion treatment on sludge, the degradation rate of organic matters can be effectively increased, the sludge hydrolytic process can be accelerated, the anaerobic digestion time of the sludge can be shortened, and the biological gas yield can be increased.

The invention discloses an organic fertilizer fermentation inoculant and application. The inoculant is composed of a primary fermentation inoculant body and a secondary fermentation inoculant body. The primary fermentation inoculant body comprises bacillus subtilis, bacillus licheniformis, trichoderma viride, aspergillus oryzae, pseudomonas, trichoderma koningii, geotrichum candidum, long spiny shells, ascomycetes, thermomyces lanuginosus and thermobifida fusca. The secondary fermentation inoculant body comprises halophilic bacteria, alkalophilic bacteria, azotobacter chroococcum, bacillus megatherium, streptomyces griseus, bacillus thuringiensis, brevibacillus laterosporus and bacillus mucilaginosus. Time for fermenting organic fertilizer with cow dung as the raw material can be effectively shortened, and diseases and insect pests in the fertilizer can be eliminated. Meanwhile, alkalophilic bacteria and bacillus thuringiensis are added so that the fermentation inoculant can adapt to saline-alkali soil and can prevent diseases and insect pests of plants, and the prepared organic fertilizer can improve the soil structure, reduces the salt content and the pH value in soil, improves soil fertility and is particularly beneficial to improvement of saline-alkali soil.

The invention relates to an engineering bacteria based on extracellular Alpha-L-arabinofuranosidase and implementation method thereof, belonging to a field of bio-engineering technology. The arabinofuranosidase is prepared by cloning arabinofuranosidase gene from Streptomyces griseus JSD-1, linking the arabinofuranosidase gene with an expression vector to obtain a recombinant expression vector, further introducing the recombinant expression vector into E. coli and carrying out induced synthesis. The deficiencies of induced expression and low expression levels in prior art are overcome and a new method is provided for the industrial fermentative production of Alpha-L-Arab furan-glucosidasethe by applying gene engineering bacteria to the expression of the arabinofuranosidase prepared by the gene according to the invention.

The invention provides a microbial fermentation bacterium capable of rapidly removing odor and fixing nitrogen used for treating human and animal feces. The microbial fermentation bacterium is composed of bacillus licheniformis, bacillus megatherium, lactobacillus acidophilus, a brewer's yeast, azotobacter chroococcum and streptomyces griseus. The microbial inoculum has the capacity of inhibitingthe growth and propagation of rot pathogens, and can achieve the deodorization effect for stink substances from the source, so that the harm caused to the human body by the pathogens can be reduced. For the microbial inoculum, the number of effective viable bacteria is high, when the materials of the same amount are treated, the feeding amount of the microbial inoculum can be reduced, thus the treatment cost for the feces can be greatly reduced, and the microbial fermentation bacterium can be widely applicable to immobile or movable toilets. After the microbial inoculum is inoculated to a feces storage box, the effects of rapidly removing odor and efficiently degrading the human and animal feces can be achieved, the feces can be degraded to form liquid within a short time, the maximal harmlessness and minimization of the feces are realized, and the policy of sustainable development is conformed.

The invention relates to a Streptomyces griseus subsp.griseus trehalose synthetase gene and applications thereof. The gene is cloned from Streptomyces griseus subsp.griseus, and the synthetase gene expressed by the gene is capable of converting maltose into trehalose. The gene has the biggest characteristics of high conversion efficiency and high reaction speed. Under the reaction condition provided by the invention, the content of the trehalose in the reaction product of the trehalose generated by converting the maltose can reach 87 percent, and moreover, only 3.8 percent of glucose is produced, thereby being beneficial to increasing the conversion rate of a substrate and reducing the production cost. Recombinant trehalose synthetase produced by applying the gene can be used for preparing trehalose by using maltose syrup sold in the market or maltose syrup prepared from starch. The trehalose can be applied to the field of food industry, facial cosmetics, pharmaceutical and biological products and the like.

The invention discloses a biological medicine for preventing and curing plant parasitic nematode and application thereof. The active ingredient of the biological medicine is Streptomyces rubrogriseus HDZ-9-47 CGMCC No 2878. The plant parasitic nematode is prevented and cured by using the biological medicine in the invention, and the results show that the Streptomyces rubrogriseus HDZ-9-47 CGMCC No 2878 and the metabolite thereof have very strong toxic activity for eggs of Meloidogyne incognita and can obviously inhibit hatching of eggs, and have obvious parasitization for eggs and egg capsules of Meloidogyne incognita; when Streptomyces rubrogriseus HDZ-9-47 CGMCC No 2878 and the metabolite thereof are mixedly used, the effect of insect disinfestation on Meloidogyne incognita can reach 100%. The greenhouse experimental results of tomatoes show that the Streptomyces rubrogriseus HDZ-9-47 CGMCC No. 2878 has very good effect of killing nematode. The biological medicine in the invention has good application development prospect for prevention and cure of plant parasitic nematode.

The invention discloses a pretreatment method for enhancing anaerobic sludge digestion by virtue of a compound microorganism bacterium agent. The pretreatment method comprises the following steps: concentrating residual sludge, adding a compound microorganism bacterium agent, performing stirring reaction, inoculating the pretreated residual sludge into an anaerobic digestion pond according to a certain ratio, and performing mesophilic anaerobic digestion on anaerobic sludge, wherein the compound microorganism bacterium agent is prepared by mixed fermentation of 5%-20% of each of bacillus subtilis, streptomyces albus, streptomyces griseus, candida utilis, aspergillus sojae, lactobacillus bulgaricus, lactococcus lactis, rhodopseudomonas palustris, rhodopseudomonas capsulate and rhodopseudomonas spheroides, the total viable count is not less than 2*108/ml, and the pH value of the compound microorganism bacterium agent is 3-4. According to the method, the sludge hydrolysis process can be accelerated, the sludge retention time can be shortened, the biological gas production rate can be increased, and the anaerobic digestion efficiency can be improved.

The invention discloses streptomyces canus; through identification, it is streptomyces canus, and the bacterial strain name is gl2-18; the streptomyces canus is preserved at China General Microbiological Culture Collection Center; the preservation number is CGMCC No. 13662; the preservation date is February 15, 2017. Compared with the prior art, the resting cell or the growth cell of the new strain streptomyces canus CGMCC 13662 can effectively degrade chloronicotinyl insecticide, in particular to acetamiprid and thiacloprid; the streptomyces canus is used as the pesticide degradation fungicide, and has very good application prospect.

The invention discloses angucycline compounds and an application of the angucycline compounds in preparation of an anti-tumour or antibacterial medicine. According to the invention, fermentation extract of marine actinomycete, namely streptomyces rubrogriseus SCSIO 11594, is separated and purified, three cytotoxic active or bacteriostatic active compounds are obtained, and the three cytotoxic active or bacteriostatic active compounds are determined to be angucycline compounds through structural analysis, and a concrete structure is shown in a formula (II) in the specification. The three compounds 1, 2 and 3 are separated from streptomyces rubrogriseus SCSIO 11594, wherein the compounds 2 and 3 have anti-tumour activity and can be used for preparing an anti-tumour medicine, and the compounds 1, 2 and 3 have antibacterial activity and can be used for preparing an antibacterial medicine, so that the angucycline compounds disclosed by the invention provide new or new-functional pilot compounds for development of new anti-tumour or antibacterial medicines and have important significance for developing China marine medicinal resources.

The invention provides a seed coating agent using cycloheximide as the effective composition, which is prepared through isolating and extracting active ingredient from the microbial cultivation substance of SPRI-201 (Streptomyces genius) CGMCC No.1317, then mixing with carrying agent and auxiliary agent.

The invention discloses actinomycetes for preventing and treating peanut diseases and application of actinomycetes .The name of strain LX14 is Streptomyces lavendulae, the preservation unit is Center of general microbiology of China Committee for Culture Collection of Microorganisms, the preservation time is January 22, 2014, and the preservation number is CGMCC No.8806 .Metabolin Streptomyces lavendulae is screened out from soil in a separation mode, is high in restraining effect on peanut fungi, is applied to preventing and treating peanut crown rot pathogen, a peanut scab disease, peanut root rot and peanut southern blight, has important significance in peanut disease preventing and treating and is very important in plant disease biological preventing and treating; a new approach is added for development of agricultural fungicide, and the Streptomyces lavendulae has good development and application prospects.

The invention provides application of bracteose eucalyptus essential oil and a complex bacterial agent thereof in preparing an insect repellent for marine fishes. The bracteose eucalyptus essential oil complex bacterial agent comprises the bracteose eucalyptus essential oil and streptomyces griseus HL-50 of which the bacterial content is not smaller than 5*10<8> CFU/g, wherein the preservation number of the streptomyces griseus HL-50 is CGMCC No.18424, and the primary effective constituent of the bracteose eucalyptus essential oil is 1,8-eucalyptol. The bracteose eucalyptus essential oil complex bacterial agent is easy to prepare and use, and the effects of effectively expelling various parasites which infect the marine fishes and inhibiting bacteria can be achieved simply by uniformly mixing the bracteose eucalyptus essential oil complex bacterial agent in edible feed for the marine fishes according to the use amount of 0.5-1.5 kg per ton of the feed and continuously feeding the fishes with the feed for 4-7 days; moreover, the complex bacterial agent is poisonless, harmless, and suitable for being used by the marine fishes for a long term, reduces the breeding cost, and has a wideapplication prospect.

The invention discloses a Eucalyptus polybractea essential oil with an anthelmintic effect, and a composite microbial agent and an application thereof. The Eucalyptus polybractea essential oil includes 1,8-cineole, beta-methylisopropylbenzene, limonene, alpha-pinene and alloaromadendrene, wherein the most important effective component of the Eucalyptus polybractea essential oil is 1,8-cineole. Theinvention also discloses the composite microbial agent prepared from the Eucalyptus polybractea essential oil and Streptomyces griseus HL-50 with the preservation number of CGMCC No.18424. The composite microbial agent can be applied to livestock and poultry breeding in order to effectively repel various parasites infecting livestock and poultry and inhibit bacteria. The essential oil has the advantages of natural components, excellent quality, and no influences on feeding and growth of animals, and the composite microbial agent also has the advantages of simplicity in preparation, safety inuse, no toxic or side effects, improvement of the growth performance and the intestinal health of livestock and poultry, reduction of the breeding cost, and wide application prospect.

The invention relates to a bioconversion fermentation process for efficiently producing corydalmine. The process includes steps of: using a streptomyces griseus with a preservation number of ATCC 13273, converting L-tetrahydropalmatine in a culture medium comprising 30 parts of corn flour, 7.6 parts of bean pulp, 5 parts of yeast powder, 5 parts of KH2PO4, 5 parts of NaCl, 0.1 part of FeSO4.7H2O and 1000ml of distilled water. The conversion product is the corydalmine. The conversion and fermentation temperature is 28 DEG C. The pH is 6.0. The aerating capacity is 1:1.2. The inoculum concentration is 6% and the stirring rate is 180r/minute. The bioconversion fermentation is carried out for four days under above conditions.

The invention relates to a zymotic fluid for biofermentation and a preparation method thereof. The zymotic fluid comprises the following components in percentage by weight: 0.3-0.8 percent of a saccharomyces sake liquid, 0.3-0.8 percent of a streptomyces griseus liquid, 0.3-0.8 percent of a lactobacillus liquid, 30-50 percent of rice wine distiller's grains, 10-30 percent of biomasses and the balance of purified water. A product can be obtained by performing static biological liquid fermentation on the components. Compared with the prior art, the zymotic fluid has the advantages of low preparation cost, high nutrient yield, extremely rich components, high stability, capability of serving as a bio-fermentation food, full replacement of a chemical synthesis nutritional additive in the conventional cosmetics by adding into skin care products, relatively low preparation cost, safe and stable quality, and fundamental solving of the problems of small quantity of nutritional substances, single or mixed components and poor stability existing in skin care products.

The invention belongs to the field of biological bacterial fertilizers, in particular to a composite microbial agent. The composite microbial agent is prepared from five types of beneficial soil bacteria, wherein bacillus subtilis (TMQK07), bacillus laterosporus (TMQC01) and lactobacillus casei (TMQR02) are mixed according to a living bacteria count proportion of (0.9 to 1) to (0.8 to 0.9) to (5 to 6), and a live bacteria count is larger than or equal to 3,000,000,000/ml; and moreover, streptomyces griseus (TMQMH05) fermentation liquor containing 700 U/ml or above of streptomycin, and trichoderma harzianum (TMQMH12) fermentation liquor with a spore count of 500,000,000/ml are mixed with a mixture of the bacillus subtilis, the bacillus laterosporus and the lactobacillus casei, and polysaccharides, gamma-polyglutamic acid and humic acid are added as synergists in order to overcome the continuous cropping of crops, lower the incidence rate of wilt, and achieve the effects of resisting bacteria, tonifying plants, preventing diseases and improving yield.

The invention discloses a decomposition maturing agent and the application thereof to green waste recycling. A fermentation broth is combined by adopting 20 to 50 parts of streptomyces griseus, 5 to 15 parts of pholiota adipose, 5 to 15 parts of mortierella alpine, 5 to 15 parts of flavobacterium columnare, 5 to 15 parts of pseudomonas stutzeri and 20 to 40 parts of rhodopseudomonas palustris; the bacterial broth is fully adsorbed on a carrier, namely crushed green waste of which the particle size is 1 to 30mm; water is added to the green waste until the water content reaches 40 percent to 70 percent, and then 100 to 300g of decomposition maturing agent are added to the green waste per cubic meter according to a volume ratio; auxiliary materials are urea and calcium superphosphate, and the weight ratio of the urea to the calcium superphosphate is 1:3; the weight ratio of the carrier to the auxiliary materials is 1:2; the decomposition maturing agent is obtained after the fermentation broth, the carrier and the auxiliary materials are evenly mixed; the decomposition maturing agent and the green waste are composted and fermented in a sealed manner at a temperature of 20 to 40 DEG; compost is turned once every 30 days, and water is supplemented to the compost until the water content reaches 40 to 70 percent when the compost is turned; and after being composted for 120 days, the green waste is spread and aired to be dried, and then organic fertilizer is obtained. The decomposition maturing agent has a wide application value.

The invention discloses a streptomycin high-yield strain and simultaneously provides a screening and fermenting method of the strain. The streptomycin high-yield strain provided by the invention is named streptomyces griseus (Streptomycesgriseus) DC622, is preserved in the Common Microorganism Center of China Committee for Culture Collection of Microorganisms (CGMCC) on June 14, 2012 and has a preservation number of CGMCCNO.6219. The screening method disclosed by the invention mainly comprises the following steps of: (a) obtaining original strain spore liquid; (b) carrying out mutagenesis of spore suspension; (c) screening high-phosphorus strains; (d) screening the high-yield strain; and (e) storing the high-yield strain. The invention also discloses a screening method of the DC622 strain. The average fermentation titer of the DC622 strain screened by the method can be up to 14908 U/mL.

The invention discloses a cytochrome P450 enzyme, a construction for electron transfer system engineering bacteria thereof and an application thereof and belongs to the technical field of biotechnology. The bacterial strain is the engineering strain which utilizes streptomyces griseus (ATCC13273) endogenous ferredoxin and ferredoxin reductase as P450 enzyme CYP105D1 electron transfer systems. The bacterial strain can be utilized to realize the specific oxygen-site demethylation for L-tetrahydropalmatine and supply the feasibility for utilizing genetic engineering strain biotransformation to produce L-corydalmine. Meanwhile, ferredoxin reductases capable of supporting CYP105D1 biological catalysis disclosed by the invention all can promote the biotransformation capacity of CYP105D1 for various natural products, can remove L-tetrahydropalmatine and can realize efficient conversion of natural products, such as, warfarin and 7-ethyoxyl coumarin. An embodiment is provided for efficient biotransformation based on P450.