Method for preparing nutritional type improved microbial inoculum special for saline-alkali soil rice planting
When planting rice on saline-alkali land, an improved fungal agent configuration method containing liquor yeast solution, Bacillus subtilis solution, Streptococcus solution and indigenous microbial solution was used to solve the growth adaptation of traditional fungal agents in saline-alkali land environment. The problem of insufficient sexuality has achieved efficient and economical rice growth effect.
Patent Information
- Application Number
- CN202411410187.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-10
- Publication Date
- 2025-02-25
AI Technical Summary
Traditional composite microbial agents have insufficient growth adaptability in high-salt and high-alkali environments in saline-alkali land, resulting in low growth activity when planting rice on saline-alkali land.
A special nutritional modified fungic agent configuration method for rice planting in saline-alkali land is adopted. By mixing the indigenous microbial bacterial solution of liquor yeast, Bacillus subtilis, Streptococcus grey and the indigenous microbial bacterial solution of saline-alkali land rice planting in saline-alkali land with rice stems, biology is used. Liquid fermentation and solid fermentation techniques cultivate active biological composite agents.
It improves the saline-alkali resistance and environmental adaptability of the active biocomposites grown in the saline-alkali land environment, reduces manufacturing costs, improves production capacity and validity period, and is easy to store and transport.
Smart Images
Figure CN119506122A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of rice planting, and in particular to a method for configuring a special nutritional improving bacterial agent for rice planting in saline-alkali land. Background Art
[0002] With the growth of population and the reduction of arable land resources, the development and utilization of saline-alkali land has become an important way to expand agricultural production space. Saline-alkali land usually contains high salt and alkaline substances, and the physical and chemical properties of the soil are poor, which is not conducive to the growth of most crops. Planting rice on saline-alkali land through a series of technical means can effectively utilize these land resources that were originally regarded as low-yield or barren land and increase food production.
[0003] The strains in traditional composite microbial agents are not well adapted to the harsh environment of saline-alkali land, such as high salt and high alkali, and the microbial growth and reproduction and soil improvement process are slow. Therefore, a method for preparing a special nutritional improvement agent for rice cultivation in saline-alkali land is provided. Summary of the invention
[0004] The purpose of the present invention is to solve the above-mentioned problems and to propose a method for preparing a special nutritional improvement bacterial agent for rice cultivation in saline-alkali land.
[0005] In order to achieve the above object, the present invention adopts the following technical scheme: the raw materials of the bacterial agent include the following components and their weight percentage contents:
[0006] (1) 0.5-0.8% liquor yeast solution (containing 5×108-5×1010 yeast);
[0007] (2) 0.5-0.8% Bacillus subtilis solution (containing 5×108-5×1010 bacteria);
[0008] (3) Streptomyces griseus solution 0.5-0.8% (bacterial content 5×108-5×1010);
[0009] (4) 0.5-0.8% bacterial solution of indigenous microorganisms in rice-growing soil of saline-alkali land (containing 5×108-5×1010 bacteria);
[0010] (5) Rice straw 85-95%;
[0011] (6) Water residue;
[0012] The method for preparing the bacterial agent comprises the following steps:
[0013] (1) Preparation of liquor yeast solution;
[0014] (2) Preparation of Bacillus subtilis solution;
[0015] (3) Preparation of Streptomyces griseus solution;
[0016] (4) Preparation of indigenous microbial culture fluid from saline-alkali rice-growing soil;
[0017] (5) Prepare the materials according to the following components and weight percentages;
[0018] (6) Preparation of active biological compounds.
[0019] Preparation of liquor yeast solution:
[0020] a. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium;
[0021] b. Preparation of triangular flask strains: Take the inclined liquor yeast strains and inoculate them into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture them at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid;
[0022] c. Preparation of production strain: Inoculate the strain liquid in a triangular flask into a fermenter containing malt juice medium at an inoculum rate of 5wt%, ferment at 35°C, with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter, and ferment for 48 hours. Then, add 1 / 3 of the volume of the fermenter under the above conditions and ferment for another 48 hours to obtain the liquor yeast solution.
[0023] Preparation of Bacillus subtilis solution:
[0024] a. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium;
[0025] b. Preparation of triangular flask strains: Take the slant Bacillus subtilis strains and inoculate them into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture them at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid;
[0026] c. Preparation of production strains: The strain liquid in a triangular flask was inoculated with 5wt% of the inoculum into a fermenter containing malt wort medium, and fermented for 48h at 35°C with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter. Then, 1 / 3 of the volume of the fermenter was added with malt wort medium under the above conditions and fermented for another 48h to obtain a Bacillus subtilis liquid.
[0027] Preparation of Streptomyces griseus solution:
[0028] a. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium;
[0029] b. Preparation of triangular flask strains: Take the inclined gray Streptomyces strains and inoculate them into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture them at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid;
[0030] c. Preparation of production strain: Inoculate the strain liquid in a triangular flask into a fermenter containing malt juice medium at an inoculum rate of 5wt%, ferment at 35°C, with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter, and ferment for 48h. Then, add 1 / 3 of the volume of the fermenter under the above conditions and ferment for another 48h to obtain a Streptomyces griseus solution.
[0031] Preparation of indigenous microbial culture liquid of rice-growing soil in saline-alkali land:
[0032] a. Extraction of indigenous microbial liquid from saline-alkali rice-growing soil: prepare a 150L bucket, first put 50L of saline-alkali rice-growing soil with good growth into the bucket, and then put about 30L of flooded rice water near the soil. Leave the bucket uncovered (but avoid rain) in a ventilated and sunny place for one day; after one day, put 30L of molasses in the bucket, stir it well, and add 10kg (L) of humic acid (powdered can be soaked and mixed with 10L of flooded rice water). Leave the bucket uncovered (but avoid rain) for three days in a ventilated and sunny place; after three days, put 500g of polyaspartic acid (powdered) and 20 tablets of any good brand of multivitamins (A, B group, multiple trace elements) into powder and stir it well to dissolve.
[0033] b. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium;
[0034] c. Preparation of triangular flask strains: Inoculate indigenous microbial strains from rice-growing soil on sloping saline-alkali land into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid;
[0035] d. Preparation of production strains: Inoculate the strain liquid in a triangular flask with a 5wt% inoculation amount into a fermenter containing malt juice medium, ferment for 48 hours at 35°C, with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter, and then add 1 / 3 of the volume of the fermenter under the above conditions. Ferment for another 48 hours to obtain the indigenous microbial strain of saline-alkali rice-growing soil.
[0036] The materials are prepared according to the following components and weight percentages: 0.5-0.8% liquor yeast solution, 0.5-0.8% bacillus subtilis solution, 0.5-0.8% griseus streptococcus solution, indigenous microbial solution of rice-growing soil in saline-alkali land, 85-95% rice straw, and the balance water.
[0037] The liquor yeast solution, Bacillus subtilis solution, Streptomyces griseus solution and the indigenous microorganism solution of rice-growing soil in saline-alkali land are mixed with rice straw fragments according to a formula, and water is added to maintain the humidity at 40-60%. The pile is covered and fermented. When the pile temperature is greater than 20°C, the rice straw fragments are transferred to a fermentation tank for further fermentation, and the pile is ventilated for 15 minutes / 10 hours, and the pile is turned over every 10 hours. Meanwhile, the pile temperature is controlled at 50-60°C and the humidity is controlled at 30-50%. After fermentation for 7 days, the pile is flattened to cool down. When the pile temperature drops to below 20°C and the humidity drops to 14-16%, an active nutritional improvement bacterial agent specially used for rice cultivation in saline-alkali land is obtained.
[0038] The malt extract medium is obtained by mixing the following components and contents in parts by weight: 0.6-1.2 of potassium nitrate; 0.3-0.7 of potassium dihydrogen phosphate; 0.3-0.7 of magnesium sulfate; 0.0005-0.001 of ferrous sulfate; 2-3 of soluble starch; 2-3 of agar powder; and 90-95 of malt extract.
[0039] Compared with the prior art, the present invention overcomes the problem that the traditional composite microbial agent is not suitable for the growth of saline-alkali land. The bacterial species used in the present invention are indigenous bacteria that assist other engineering bacteria in co-cultivation and training, and the natural properties remain unchanged. The active biological composite agent that can improve the growth of rice in the saline-alkali land environment is made of rice straw powder as the main bacterial agent and carrier, and white wine yeast, Bacillus subtilis, Streptomyces griseus and indigenous microorganisms of rice-growing soil in saline-alkali land are selected as the main fermentation agents. It uses biological liquid fermentation and solid fermentation technology to cultivate white wine yeast, Bacillus subtilis, indigenous microorganisms of rice-growing soil in saline-alkali land and Streptomyces griseus biological agents, has low manufacturing cost, high production capacity, long effective period, strong stability, simple storage conditions, convenient transportation, different bacterial species can be composited and compatible, has a wide range of salt-alkali resistance, and is an industrial-grade domesticated solid active biological composite agent with strong environmental tolerance elasticity, which fundamentally solves the problem of weak salt-alkali resistance. BRIEF DESCRIPTION OF THE DRAWINGS
[0040] Figure 1 This is a schematic diagram of the steps of a method for preparing a nutritional improvement bacterial agent specifically for rice cultivation in saline-alkali land proposed by the present invention. DETAILED DESCRIPTION
[0041] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.
[0042] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.
[0043] Reference Figure 1 , a method for preparing a special nutritional improvement microbial agent for rice cultivation in saline-alkali land, the raw materials of the microbial agent include the following components and their weight percentage contents:
[0044] (1) 0.5-0.8% liquor yeast solution (containing 5×108-5×1010 yeast);
[0045] (2) 0.5-0.8% Bacillus subtilis solution (containing 5×108-5×1010 bacteria);
[0046] (3) Streptomyces griseus solution 0.5-0.8% (bacterial content 5×108-5×1010);
[0047] (4) 0.5-0.8% bacterial solution of indigenous microorganisms in rice-growing soil of saline-alkali land (containing 5×108-5×1010 bacteria);
[0048] (5) Rice straw 85-95%;
[0049] (6) Water residue;
[0050] The method for preparing the bacterial agent comprises the following steps:
[0051] (1) Preparation of liquor yeast solution;
[0052] (2) Preparation of Bacillus subtilis solution;
[0053] (3) Preparation of Streptomyces griseus solution;
[0054] (4) Preparation of indigenous microbial culture fluid from saline-alkali rice-growing soil;
[0055] (5) Prepare the materials according to the following components and weight percentages;
[0056] (6) Preparation of active biological compounds.
[0057] Preparation of liquor yeast solution:
[0058] a. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium;
[0059] b. Preparation of triangular flask strains: Take the inclined liquor yeast strains and inoculate them into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture them at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid;
[0060] c. Preparation of production strain: Inoculate the strain liquid in a triangular flask into a fermenter containing malt juice medium at an inoculum rate of 5wt%, ferment at 35°C, with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter, and ferment for 48 hours. Then, add 1 / 3 of the volume of the fermenter under the above conditions and ferment for another 48 hours to obtain the liquor yeast solution.
[0061] Preparation of Bacillus subtilis solution:
[0062] a. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium;
[0063] b. Preparation of triangular flask strains: Take the slant Bacillus subtilis strains and inoculate them into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture them at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid;
[0064] c. Preparation of production strains: The strain liquid in a triangular flask was inoculated with 5wt% of the inoculum into a fermenter containing malt wort medium, and fermented for 48h at 35°C with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter. Then, 1 / 3 of the volume of the fermenter was added with malt wort medium under the above conditions and fermented for another 48h to obtain a Bacillus subtilis liquid.
[0065] Preparation of Streptomyces griseus solution:
[0066] a. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium;
[0067] b. Preparation of triangular flask strains: Take the inclined gray Streptomyces strains and inoculate them into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture them at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid;
[0068] c. Preparation of production strain: Inoculate the strain liquid in a triangular flask into a fermenter containing malt juice medium at an inoculum rate of 5wt%, ferment at 35°C, with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter, and ferment for 48h. Then, add 1 / 3 of the volume of the fermenter under the above conditions and ferment for another 48h to obtain a Streptomyces griseus solution.
[0069] Preparation of indigenous microbial culture liquid of rice-growing soil in saline-alkali land:
[0070] a. Extraction of indigenous microbial liquid from saline-alkali rice-growing soil: prepare a 150L bucket, first put 50L of saline-alkali rice-growing soil with good growth into the bucket, and then put about 30L of flooded rice water near the soil. Leave the bucket uncovered (but avoid rain) in a ventilated and sunny place for one day; after one day, put 30L of molasses in the bucket, stir it well, and add 10kg (L) of humic acid (powdered can be soaked and mixed with 10L of flooded rice water). Leave the bucket uncovered (but avoid rain) for three days in a ventilated and sunny place; after three days, put 500g of polyaspartic acid (powdered) and 20 tablets of any good brand of multivitamins (A, B group, multiple trace elements) into powder and stir it well to dissolve.
[0071] b. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium;
[0072] c. Preparation of triangular flask strains: Inoculate indigenous microbial strains from rice-growing soil on sloping saline-alkali land into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid;
[0073] d. Preparation of production strains: Inoculate the strain liquid in a triangular flask with a 5wt% inoculation amount into a fermenter containing malt juice medium, ferment for 48 hours at 35°C, with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter, and then add 1 / 3 of the volume of the fermenter under the above conditions. Ferment for another 48 hours to obtain the indigenous microbial strain of saline-alkali rice-growing soil.
[0074] The materials are prepared according to the following components and weight percentages: 0.5-0.8% liquor yeast solution, 0.5-0.8% bacillus subtilis solution, 0.5-0.8% griseus streptococcus solution, indigenous microbial solution of rice-growing soil in saline-alkali land, 85-95% rice straw, and the balance water.
[0075] The liquor yeast solution, Bacillus subtilis solution, Streptomyces griseus solution and the indigenous microorganism solution of rice-growing soil in saline-alkali land are mixed with rice straw fragments according to a formula, and water is added to maintain the humidity at 40-60%. The pile is covered and fermented. When the pile temperature is greater than 20°C, the rice straw fragments are transferred to a fermentation tank for further fermentation, and the pile is ventilated for 15 minutes / 10 hours, and the pile is turned over every 10 hours. Meanwhile, the pile temperature is controlled at 50-60°C and the humidity is controlled at 30-50%. After fermentation for 7 days, the pile is flattened to cool down. When the pile temperature drops to below 20°C and the humidity drops to 14-16%, an active nutritional improvement bacterial agent specially used for rice cultivation in saline-alkali land is obtained.
[0076] The malt extract medium is obtained by mixing the following components and contents in parts by weight: 0.6-1.2 of potassium nitrate; 0.3-0.7 of potassium dihydrogen phosphate; 0.3-0.7 of magnesium sulfate; 0.0005-0.001 of ferrous sulfate; 2-3 of soluble starch; 2-3 of agar powder; and 90-95 of malt extract.
[0077] Compared with the prior art, the present invention overcomes the problem that traditional composite microbial agents are not suitable for growth in saline-alkali lands, and the strains used are indigenous bacteria that assist other engineered bacteria in co-cultivation and training, and the natural properties remain unchanged. The active biological composite agent that uses rice straw crushed powder as the main bacterial agent and carrier, and selects white wine yeast, Bacillus subtilis, Streptomyces griseus and indigenous microorganisms of rice-growing soil in saline-alkali land as the main fermentation agents, can improve the growth of rice in saline-alkali land environments. It uses biological liquid fermentation and solid-state fermentation technology to cultivate white wine yeast, Bacillus subtilis, indigenous microorganisms of rice-growing soil in saline-alkali land and Streptomyces griseus biological agents, has low manufacturing cost, high production capacity, long effective period, strong stability, simple storage conditions, convenient transportation, different strains can be compounded into one, has a wide range of salt-alkali resistance, and strong environmental tolerance elasticity. It is an industrial-grade domesticated solid active biological composite agent that fundamentally solves the problem of weak salt-alkali resistance.
[0078] The above are only preferred specific implementation modes of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.
Claims
1. A method for preparing a special nutritional improvement bacterial agent for rice cultivation in saline-alkali land, characterized in that: The raw materials of the microbial agent include the following components and their weight percentage contents: (1) 0.5-0.8% liquor yeast solution (containing 5×108-5×1010 yeast); (2) 0.5-0.8% Bacillus subtilis solution (containing 5×108-5×1010 bacteria); (3) Streptomyces griseus solution 0.5-0.8% (bacterial content 5×108-5×1010); (4) 0.5-0.8% bacterial solution of indigenous microorganisms in rice-growing soil of saline-alkali land (containing 5×108-5×1010 bacteria); (5) Rice straw 85-95%; (6) Water residue; The method for preparing the bacterial agent comprises the following steps: (1) Preparation of liquor yeast solution; (2) Preparation of Bacillus subtilis solution; (3) Preparation of Streptomyces griseus solution; (4) Preparation of indigenous microbial culture fluid from saline-alkali rice-growing soil; (5) Prepare the materials according to the following components and weight percentages; (6) Preparation of active biological compounds.
2. The method for preparing a special nutritional improving bacterial agent for rice planting in saline-alkali land according to claim 1, characterized in that: Including the preparation of liquor yeast liquid: a. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium; b. Preparation of triangular flask strains: Take the inclined liquor yeast strains and inoculate them into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture them at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid; c. Preparation of production strain: Inoculate the strain liquid in a triangular flask into a fermenter containing malt juice medium at an inoculum rate of 5wt%, ferment at 35°C, with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter, and ferment for 48 hours. Then, add 1 / 3 of the volume of the fermenter under the above conditions and ferment for another 48 hours to obtain the liquor yeast solution.
3. The method for preparing a special nutritional improving bacterial agent for rice planting in saline-alkali land according to claim 1, characterized in that: Including the preparation of Bacillus subtilis liquid: a. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium; b. Preparation of triangular flask strains: Take the slant Bacillus subtilis strains and inoculate them into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture them at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid; c. Preparation of production strains: The strain liquid in a triangular flask was inoculated with 5wt% of the inoculum into a fermenter containing malt wort medium, and fermented for 48h at 35°C with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter. Then, 1 / 3 of the volume of the fermenter was added with malt wort medium under the above conditions and fermented for another 48h to obtain a Bacillus subtilis liquid.
4. The method for preparing a special nutritional improving bacterial agent for rice planting in saline-alkali land according to claim 1, characterized in that: Including the preparation of Streptomyces griseus solution: a. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium; b. Preparation of triangular flask strains: Take the inclined gray Streptomyces strains and inoculate them into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture them at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid; c. Preparation of production strain: Inoculate the strain liquid in a triangular flask into a fermenter containing malt juice medium at an inoculum rate of 5wt%, ferment at 35°C, with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter, and ferment for 48h. Then, add 1 / 3 of the volume of the fermenter under the above conditions and ferment for another 48h to obtain a Streptomyces griseus solution.
5. The method for preparing a special nutritional improving bacterial agent for rice planting in saline-alkali land according to claim 1, characterized in that: Including the preparation of indigenous microbial liquid of saline-alkali rice-growing soil: a. Extraction of indigenous microbial liquid from saline-alkali rice-growing soil: prepare a 150L bucket, first put 50L of saline-alkali rice-growing soil with good growth into the bucket, and then put about 30L of rice water from the nearby soil. Leave the bucket uncovered in a ventilated and sunny place for one day; after one day, put 30L of molasses in the bucket, stir well and evenly, and 10kg (L) of humic acid. Leave the bucket uncovered in the sun for three days; after three days, put 500g of polyaspartic acid and 20 tablets of any good brand of multivitamins in the bucket, grind them into powder and put them in, stir well and dissolve. b. Preparation of liquid culture medium: malt extract medium was selected as the nutrient source, and sterilized at 0.15 MPa, 121°C for 15 min to obtain sterilized malt culture medium; c. Preparation of triangular flask strains: Inoculate indigenous microbial strains from rice-growing soil on sloping saline-alkali land into a triangular flask containing sterilized malt culture medium in a sterile environment, and culture at 35°C and a vibration rate of 100-200 rpm for 48 hours to obtain triangular flask strain liquid; d. Preparation of production strains: Inoculate the strain liquid in a triangular flask with a 5wt% inoculation amount into a fermenter containing malt juice medium, ferment for 48 hours at 35°C, with a ventilation ratio of 1:1 (V / V) and a liquid volume of 1 / 3 of the volume of the fermenter, and then add 1 / 3 of the volume of the fermenter under the above conditions. Ferment for another 48 hours to obtain the indigenous microbial strain of saline-alkali rice-growing soil.
6. The method for preparing a special nutritional improving bacterial agent for rice cultivation in saline-alkali land according to claim 1, characterized in that: The materials are prepared according to the following components and weight percentages: 0.5-0.8% liquor yeast solution, 0.5-0.8% bacillus subtilis solution, 0.5-0.8% griseus streptococcus solution, indigenous microbial solution of rice-growing soil in saline-alkali land, 85-95% rice straw, and the balance water.
7. The method for preparing a special nutritional improving bacterial agent for rice cultivation in saline-alkali land according to claim 1, characterized in that: The method comprises the steps of mixing liquor yeast solution, Bacillus subtilis solution, Streptomyces griseus solution and microorganism solution of soil indigenous to rice cultivation in saline-alkali land into rice straw fragments according to a formula, adding water to maintain a humidity of 40-60%, covering the pile for fermentation, and when the pile temperature is greater than 20 DEG C, moving the rice straw fragments into a fermentation tank for further fermentation, ventilating the pile for 15 minutes / 10 hours, turning the pile every 10 hours, and controlling the pile temperature at 50-60 DEG C and the humidity at 30-50%. After fermentation for 7 days, the pile is flattened for cooling, and when the pile temperature drops to below 20 DEG C and the humidity drops to 14-16%, an active nutritional improved bacterial agent specially used for rice cultivation in saline-alkali land is obtained. The malt extract medium is obtained by mixing the following components and contents in parts by weight: 0.6-1.2 of potassium nitrate; 0.3-0.7 of potassium dihydrogen phosphate; 0.3-0.7 of magnesium sulfate; 0.0005-0.001 of ferrous sulfate; 2-3 of soluble starch; 2-3 of agar powder; and 90-95 of malt extract.