12 results about "Stolon" patented technology

The invention relates to the technical field of plant genetic engineering, and discloses a strawberry stolon regulation gene ZFP6 and application thereof, and the nucleotide sequence of the gene is as shown in SEQ ID NO.1. Researches prove that the ZFP6 gene is specifically expressed in axillary meristem of strawberry in the adult-plant stage, and the GA20ox4 gene can be directly or indirectly activated to start a biosynthesis pathway of GA and drive axillary buds to develop towards stolons. Through genetic interaction analysis of the ZFP6 gene, the GA20ox4 gene and the RGA1 gene, the function of the ZFP6 gene as an axillary bud fate decision upstream regulation factor is further verified, the blank of an endogenous regulation mechanism of the strawberry stolons is filled, and the ZFP6 gene is proved to be a molecular target for specifically regulating formation of the strawberry stolons. The gene sequence, the primer, the gene knockout expression vector and the matched technical system provided by the invention lay a foundation for strawberry molecular breeding and industrial application.

The invention discloses a strawberry two-step cutting seedling raising method based on stolon double-source water supply. The strawberry two-step cutting seedling raising method comprises the following steps: s1, hole tray installation; s2, collecting seedlings; s3, performing two-step cuttage, namely inserting the seedling obtained in the step s2 into a matrix of a hole of a seedling tray, rotating a limiting strip on the seedling tray to a position above a hole adjacent to the seedling cuttage hole, bending a stolon section connected with the seedling towards one side, penetrating through a limiting hole in the limiting strip, and inserting the stolon section into the matrix of the hole, wherein the limiting holes are used for stabilizing stolons; s4, after cutting is completed, sunshade treatment is not carried out in the cultivation period; and s5, cutting the seedlings, namely cutting off connected stolons from the bases of the seedlings by using disinfected scissors, and pulling out the cut stolons. According to the method, double-source water supply is adopted, the survival rate of the seedlings is improved, the root system development is rapid, and a seedling raising hole tray is designed in a matched manner, so that the operations of cuttage, transplanting and the like are more convenient.

A new and distinct Fragaria x ananassa plant named ‘Finette’ which exhibits an upright growth habit with moderately dense foliage and a relative abundance of stolons. ‘Finette’ is characterized by large foliage born on broadly outreaching stolons, an abundance of red berries with achenes that are level with the firm flesh which has a pleasantly sweet, non-acidic flavor proven to be consistently produced from late April to early November while maintaining their large conical size. ‘Finette’ has shown stability of these characteristics from generation to generation.

The application provides a method for creating a strawberry germplasm for controlling weeds and application thereof, and belongs to the technical field of biological breeding. Forest strawberry 'Ruegen' is used as a female parent, and yellow hair strawberry is used as a male parent, F1 generation is obtained by hybridization, F2 generation is obtained by screening selfing of the F1 generation, and the F2 generation is screened to obtain a strain with less flowering, no fruiting, easy to sprout stolon and easy to tiller, which is the strawberry for controlling weeds. The forest strawberry 'Ruegen' with strong resistance and not sprouting stolon is crossbred with the yellow hair strawberry with easy sprouting stolon, the offspring is selected to have easy sprouting stolon, easy tillering and only flowering, and the strain is transplanted and applied to orchards and woodland, so that the growth of weeds can be inhibited.

This invention discloses a sand-fixing method utilizing coppicing and sand burial of Haloxylon ammodendron, relating to the fields of windbreak, sand fixation, and vegetation restoration. Based on the natural growth angle of Haloxylon ammodendron branches, this invention applies coppicing and layering treatments to upright and inclined branches respectively. Inclined branches are laid flat against the ground and buried in sand, causing adventitious roots to sprout from the buried internodes and new suckers to grow upwards. These newly formed suckers are guided towards adjacent plants and buried perpendicular to the prevailing wind direction, creating a connected network structure between plants through their root systems and stolons. This invention classifies and treats branches according to their type. Coppicing upright branches reduces plant height and branch height, promoting tillering; sand burial of inclined branches induces adventitious roots, expands the water absorption area, lowers the center of gravity of branches and leaves, and increases ground cover, which is beneficial for improving the sand-fixing efficiency of vegetation, enhancing the water use efficiency of Haloxylon ammodendron, and promoting healthy shrub development.

The invention discloses a special nutrient solution for hydroponic culture of arrowheads. A hydroponic method for arrowheads comprises the following steps that in the period from field planting to stolon growing, a first nutrient solution is adopted for hydroponic planting, the pH ranges from 6.2 to 6.4, the electrical conductivity (EC) ranges from 1.3 mS / cm to 1.5 mS / cm, and the mass ratio of nitrogen, phosphorus and potassium fertilizer is 1: (0.29-0.31): (1.49-1.54); a second nutrient solution is adopted for water culture in the period from stolon extraction to corm expansion, the pH is 6.6-6.8, the conductivity is 1.5-1.7 mS / cm, and the mass ratio of nitrogen to phosphorus to potassium is 1: (0.44-0.46): (1.9-2.1). The hydroponic method is designed according to the nutritional requirements and growth characteristics of the arrowhead in the growth stage. According to the water culture method, the proportion, pH and EC of the nitrogen-phosphorus-potassium fertilizer are synergistically regulated and controlled in stages, nitrogen-phosphorus-potassium synergy and nutrient balance are achieved, and the nutrient utilization rate is high.

The application discloses a tissue culture method of strawberry, and belongs to the technical field of strawberry tissue culture, and the specific method is as follows: a stolon or a leaf of a strawberry plant is selected as an explant, the explant is subjected to disinfection treatment, and then is subjected to primary culture, subculture and rooting and seedling training to obtain tissue culture seedlings; the rooting and seedling training medium and the method of rooting and seedling training are used for training the adaptability of the root system and the opening and closing ability of the leaf stomata at the same time, so that the obtained tissue culture seedlings can quickly adapt to the soil and air environment after being transplanted to a substrate, and can be directly grown in the open air, and the propagation cycle is shortened.

The application discloses an AtYUCCA1 gene, an expression protein thereof and application of the expression protein in enhancing potato branching, identifies a new gene AtYUCCA1 for enhancing potato branching and tuber number, and the nucleotide sequence of the gene is shown as SEQ ID NO. 1. The application constructs the AtYUCCA1 into an overexpression vector pPZP222, constructs a recombination vector, transforms the recombination vector into agrobacterium, soaks potato stems in a resuspension solution of the agrobacterium to perform genetic transformation of the potato, and realizes efficient expression in vivo of the potato. The application promotes the growth and development of the potato by overexpressing the AtYUCCA1 in the potato plant. It is found that overexpression of the AtYUCCA1 promotes the branching of the potato, the production of stolon stems and the branching of underground stolon stems, and increases the number of underground tubers of the potato.

The invention relates to the technical field of tissue culture, in particular to a tissue culture method taking centella asiatica inflorescence as an explant. Firstly, centella creeping stems are cultured to obtain aseptic seedlings, non-flowering inflorescences of the aseptic seedlings serve as explants, tissue culture of centella is conducted, tissue cells of the non-flowering inflorescences are vigorous in division activity and good in development, and the induction success rate of centella can be increased; meanwhile, non-flowering inflorescences of aseptic seedlings are used as explants for tissue culture, the disinfection success rate of the centella asiatica explants can be effectively increased, the induction period in the tissue culture process is shortened, and therefore the propagation efficiency of centella asiatica is improved.

The invention discloses a method for cultivating pterocladia caerulea. Relates to the field of seaweed industrialized seedling production. The cultivation method of the pterocladia capillipes comprises the following steps: S1, taking the pterocladia capillipes, and performing disinfection and crushing treatment to obtain pterocladia capillipes frond particles; s2, spreading the Prassica rapa frond particles on an adhesion substrate; s3, placing the substratum in an oligotrophic water body for standing culture until stolons and rhizoids grow on the Prassica rapa frond particles and are firmly attached to the substratum; and S4, transferring the substratum attached with the brassica rapa frond into a seawater environment for culturing. According to the method disclosed by the invention, by crushing into particles and optimizing the culture conditions, the seedling raising period from sowing to complete attachment of the pterocladia caerulea is shortened to about 40 days and is shortened by more than 33% compared with that of a traditional tissue culture seedling raising method (about 60 days), the bottlenecks of long seedling raising period and low efficiency in the traditional technology are solved, and the time requirement of factory-like batch seedling raising can be met.

The application relates to a creeping stem plant cleaning device, which belongs to the technical field of agricultural machinery equipment and comprises a frame body, a stem and leaf collecting mechanism, a film collecting mechanism and a root system collecting mechanism. The frame body is used for driving the stem and leaf collecting mechanism, the film collecting mechanism and the root system collecting mechanism to move. The stem and leaf collecting mechanism is used for collecting the stems and leaves of crops. The residual film collecting mechanism is used for collecting residual films. The root system collecting mechanism is used for collecting the roots and stems of crops. The stems and leaves of crops are transported to the stem and leaf collecting assembly by a stem and leaf conveying belt, the stems and leaves of crops are collected and packed, time and labor are saved, the roots and stems of crops are dug up by a shovel knife, the dug-up roots and stems of crops are placed on a root conveying belt and transported to a root-soil separating mechanism, the soil on the roots and stems of crops is removed, the roots and stems of crops are prevented from remaining in the soil to cause insect pests, meanwhile, the mulching films on the land ridge are collected, and the collection efficiency of the roots, stems, leaves and mulching films of crops is improved.

The application discloses a method for improving growth of strawberry offspring plants based on nano CeO2, and comprises the following steps: S1, culture soil preparation; S2, culture of strawberry mother plants; S3, culture of first-generation strawberry daughter plants; and S4, culture of second-generation strawberry daughter plants. The method can culture two generations of strawberry daughter plants through the method of stolon reproduction, and a higher nano CeO2 transport coefficient can be obtained in the two generations of strawberry daughter plants. The transported nano CeO2 significantly improves the underground and aboveground biomass of the first-generation / second-generation strawberry daughter plants, thereby finally improving the crop yield. Only suitable nano CeO2 needs to be applied in the culture soil of the strawberry mother plants, and no nano CeO2 needs to be applied in the culture soil of the strawberry daughter plants, so that the cost is saved, the possible side effects of the nano CeO2 can be avoided, and the method has strong practicability.