166 results about "Stolon" patented technology

The invention provides a method for rapidly culturing shoot tips in vitro, which is characterized in that in the first step, selecting stolon of the strawberries sprouted at the same year, washing to be clean and disinfecting; in the second step, cutting 0.1-0.5 mm shoot tips of the disinfected and cleaned strawberries stolon under the anatomical lens, inoculating to a culture medium for germination culture, cutting the new sprouts after the 0.5-1.5 cm new sprouts are grown on the shoot tips, inoculating to an enrichment medium for inducing clustered shoots; in the third step, cutting the cluster buds to simple buds, inoculating to a rooting medium for rooting culture; transferring test tube plantlets to a substrate formed by mixing perlite and peat, watering and covering a plastic film for insulation and moisture retention, revealing the plastic film for gradually hardening, transferring the seedling to a plastic potted tray filled with pure peat, watering and covering the plastic film for insulation and moisture retention, and directly planting the seedling to a field. The invention has the advantages of high reproductive rate, rapid reproduction speed and high survival rate.

The invention provides a method for quickly planting cynodon dactylon on an ecological concrete slope. The method comprises the following steps of 1 slope surface preparing; 2 lattice frame constructing, wherein multiple lattice frames adjacent to one another are poured and are all backfilled with planting matrixes, the planting matrixes are leveled, tamped and covered with first non-woven fabric; 3 ecological concrete pouring; 4 cynodon dactylon planting, wherein the stolons of cynodon dactylon are sheared into cynodon dactylon stems; 5 curing. According to the method, the cynodon dactylon which is good in stress resistance, resistant to trampling and poor soil and high in recovery capacity is adopted to serve as the planting species, the survival rate is high, and the selected cynodon dactylon is at least two years old; after twenty days, the coverage rate of the cynodon dactylon can reach 80 percent, the cynodon dactylon stems grow well, adventitious roots are grown, axillary buds on the stems begin to grow into branches on the ground, and new stolons begin to tiller; after thirty days, the vegetation coverage rate is more than 95 percent. By adopting the method, vegetation landscapes can be quickly formed, the slope ecology environment can be improved, and slope ecology protection can be achieved.

The invention discloses a method for strawberry seedling breeding, which is characterized by comprising the following steps: step 1, breeding original seedlings in nutrition pots until the original seedlings enter into a reproduction and growth stage; step 2, arranging seedling-breeding ridges according to a propagation coefficient for the nutrition pots filled with the original seedlings and a breeding hole tray, i.e. placing the nutrition pots into the breeding hole tray, then filling nutrition soil, compacting the nutrition soil and leveling with the breeding hole tray surface to grow the seedlings; and step 3, after sprouts at the top end of the original seedling stolon begin to root, carrying out fixing treatment so that the sprouts at the top end of the stolon grow in the nutrition pots in breeding hole tray meshes to form the independent seedlings finally. The seedlings can be grown in a greenhouse according to the mode in prior art, therefore, the time and labor are saved and the seedlings are convenient to manage and classify; and the high-quality nutrition pot breeding seedlings can be grown outdoors by breaking through the conventional method, therefore, the investment is saved, the breeding number of the high-quality seedlings is increased, the seedling cost of planters is lowered and the high-quality seedling rate can reach more than 95%.

The invention relates to a strawberry seedling raising method, and in particular relates to a two-stage seedling raising method for strawberries, wherein the first-stage management includes from the last ten-day of August to the first ten-day of September, field planting strawberry seedlings, wherein eight thousand strawberry seedlings are planed per mu, and performing cuttage on the strawberry juvenile seedlings when entering November, wherein stolons with three to four strawberry juvenile seedlings are selected for cuttage; the second-stage management includes: performing protection management on the seedlings from December to February, performing fertilization management from February to May, until the vigorous growing periods of the stolons, and selecting the stolons with three to four strawberry juvenile seedlings for cuttage. According to the two-stage seedling raising method for strawberries, the seedlings for cottage is cultivated form two times, the seedling raising time is prolonged, the seedling resources are reasonably and fully utilized, and thereby the purpose of raising seedling with high yield, quality and efficiency is achieved; as the seedlings are planted early, the stock plants are sufficient in nutrition and can grow sufficiently, and the stolons grows vigorously and can simultaneously grow 15-20 seedlings, even up to 36 seedlings; the two-stage seedling raising method for strawberries is simple and convenient to implement, large in seedling yield, high in propagation coefficient, good in quality of cutting seedlings, and high in cuttage survival rate.

The invention discloses a novel method for efficient and rapid seedling establishment of strawberry stolons. The method comprises the following seedling establishment steps of strawberry stolon inducing, stolon cutting, grading and preprocessing, aerial root inducing, transplanting and seedling establishment. The method can be used for efficient and rapid seedling establishment of elevated stolons of excellent new strawberry species of Hongyan, Zhangji and the like. According to the novel method, the defect that rooting of stolons formed early is difficult is overcome, and by inducing the early stolons to generate aerial roots and then transplanting the stolons to a substrate and adopting a mode of short-term darkness culture, efficient and rapid seedling establishment of the strawberry stolons is achieved. By means of the novel method, the seedling establishment rate is generally 85%-89% or above, and the advantages of being simple, convenient to operate, high in seedling establishment speed and the like are achieved. Compared with cuttage, the method for inducing the stolons to generate the aerial roots is simple and convenient, and needed time is short and concentrated; the quality of offspring stolon seedlings generated through the method in different times and different batches is high and more stable.

The present invention relates to a strawberry detoxification tissue culture rapid propagation technology, and belongs to the technical field of agricultural bioengineering. The technology comprises: taking stolon tip of strawberry as explant, and carrying out inducing proliferation, secondary culture, rooting culture, transplanting acclimation and other steps so as to rapidly propagate detoxified tissue culture seedlings. According to the present invention, the formula of the culture medium is adjusted, and the stolon tip growth point is adopted to carry out inducing culture so as to achieve direct inducting budding without the callus growth stage, prevent and control varietal character variation and easily perform inoculation operation; characteristics of scientific sampling time, low inoculation contamination rate, high survival rate, simple, rapid and complete detoxification, rapidness, low cost, strong practicality and the like are provided, wherein the propagation coefficient achieves 5-8 times; and the technology is suitable for industrialized seedling breeding, and can be adopted for commercialized production.

The invention discloses a strawberry cultivating device and method. The device comprises a cultivation frame the longitudinal cross section of which is trapezoidal. The top surface of the cultivation frame is provided with a cultivation slot along the length direction of the top surface, and plugs are paved on the angular surfaces of both sides of the cultivation frame. The method comprises the following steps of: planting strawberry mother plants in the cultivation slot; fixing the strawberry mother plants in the plugs when stolon bud grow to two blades with one terminal bud; alternatively carrying out drop irrigation on the mother plants by adopting a first culture solution and a second culture solution before daughter plants take roots; and respectively carrying out drop irrigation and spray on the root systems of the mother plants and the daughter plants by adopting a third culture solution after the daughter plants take roots until the mother plants and the daughter plants grow into seedlings. The cultivation slot and the plugs of the strawberry cultivating device are far away from the ground so that the influence of plant diseases and insect pests and the soil environment can be eliminated, the controllability is strong, the management becomes convenient and the land use ratio is high. In the method, different culture solutions are dropped and irrigated or sprayed to the mother plants and the daughter plants in different periods, which not only causes little waste on the used culture solutions but also is suitable for the mother plants and the daughter plants to grow. The fruited strawberry seedlings have the advantages of complete and developed roots, strong stem bases and high carbohydrate content.

The invention relates to a method for inducing strawberry stolons and eliminating viruses in a test tube by high temperature processing in combination with shoot tip culture. In the method, the stolons of test-tube plantlets of an excellent new variable variety generated by callus differentiation of deep-mountain strawberry petals are induced in the test tube and the virus elimination of the variety is studied by combining high temperature and shoot tips in the test tube to obtain virus-free strawberry seedlings for production. The method comprises the following steps: inducing the stolons of the obtained new variety in a culture medium in the test tube, wherein the induction rate is above 99.5 percent; culturing the test-tube plantlets containing the stolons for 85d in the test tube at the temperature of 56 DEG C, and cutting 1mm of shoot tip to induce shoot tip calluses in the culture medium; then transferring the shoot tip calluses to the culture medium to perform re-differentiation culture of callus seedlings; and detecting the viruses of the regenerated seedlings, wherein the virus elimination rate is 100 percent. A test result shows that the virus elimination method by high-temperature processing in combination with the shoot tips in the test tube can eliminate the viruses completely so as to establish a strawberry virus elimination system.

The invention provides an elevation cultivation method for strawberry production and seedling culture and a facility of the method. The elevation cultivation method comprises the steps of implement of colonization planting, production and picking, and seedling culture management, wherein implement of colonization planting means colonization planting of stock plants; production and picking are conventional technical methods; the seedling culture management comprises young seedling generating period management and young seedling temporary planting management, wherein the young seedling generating period management comprises the sub-steps of spraying gibberellin once in the middle of April and late April respectively, keeping a substrate moist in the generating period of the large quantity of young seedlings, and enhancing the fertilizer and water management; hanging the young seedlings on the two sides of a seedling culture frame after generation of the young seedlings. The young seedling temporary planting management comprises the steps of cutting stolons off the roots of the stock plants after the young seedlings are all hung on the culture frame, and carrying out plug transplant on the young seedlings. By means of the elevation cultivation method, strawberry seedling cultivation and strawberry production are combined to improve the utilization rate of strawberry seedling culture equipment and soil and increase economic income of farmers, and meanwhile the operation and management of strawberry production and seedling culture are conveniently carried out by the farmers.

The invention discloses a method for remediating lead-contaminated soil or water, which comprises the following steps: (1) collecting Polygonum criopolitanum; (2) planting Polygonum criopolitanum in soil or water; (3) periodically harvesting the Polygonum criopolitanum and part of stolons above the ground or water; (4), continuing to plant and harvest the Polygonum criopolitanum till the lead content of the soil or water reaches a safe standard; and (5) performing pollution-free disposal on the harvested Polygonum criopolitanum. In the method of the invention, the Polygonum criopolitanum is planted in the lead-contaminated soil or water, so extraction can be performed continuously, the remediation process of the soil and water is simplified, and the lead contamination problem of soil can be solved radically. The method of the invention is low in cost and high in maneuverability and avoids damaging the structure of the environmental soil and causing secondary pollution.

The invention relates to a method for planting green lawn for landscaping, slope protection of roads and courtyard landscape, in particular to a method for planting green lawn by using stolons of Potentilla centigrana Maxim. In the plantation period, the stolons with at least four stalk nodes are selected, and every two stolons on each plant are not cut and are connected through a mother plant root system. The method comprises the following steps of: preparing soil; ditching and watering; completely permeating water in the ditch, horizontally putting the selected stolons into the ditch, covering fine soil with the thickness of 1 to 2 cm, and making leaves on each stalk node exposed out of the soil; finishing the work of cuttage and stem burying, covering by using a shading net; performing field management, removing the shading net after new roots and leaves are grown; and forming the newly planted lawn within 40 to 50 days. The reproduction coefficient of the Potentilla centigrana Maxim is improved by 16 times compared with that of the traditional method, seedlings are greatly saved, the shortage of the seedlings is relieved and the reproduction cost is reduced.

The invention discloses a high yield planting method of potatoes, comprising the steps of: (1) selecting fine breeds; (2) early seeding in real time, advancing about one week than usual, thereby avoiding frost coming at the end of May, because of frost resistance of potato seedings, furthermore missing origin time of plant diseases and insect pests, wherein the origin time of soil insect generally in July, while the potato seedings are easy to be damaged by insect pests, if seeding is advanced, tubers are not easy to meet with insect pest; (3) culturing in high ridge, which is beneficial for loosening soil, increasing live soil layer to 5 cm or so, and is also beneficial for stolon extending, the tuber being increased by 1 cm, meanwhile increasing ground temperature by 5 DEG C, thereby meeting with required temperature of early rhizoma growth; (4) fertilizing. The invention has the advantages of increasing production more than 50%, increasing quality of potatoes, thereby increasing commodity rate through the cultivation technique.

The invention relates to an efficient detoxification tissue cultivating method of strawberries, which takes the micro stem tips at the top of strawberry stolons as explants to conduct tissue cultivating to obtain a strawberry plant. According to the method provided by the invention, the germination rate of the micro stem tips is above 95 percent, and rooting percentage is above 98 percent. The method conducts rapid propagation by using the micro stem tips, the propagation coefficient is higher, original variety character is well kept, and the application prospect of new superior varieties strawberry plant short-term expanding propagation and rapid propagation of plants saved in a strawberry genebank is wide; furthermore, the method can be used as a new transgenosis way.

The invention relates to the technical field of planting, and provides a breeding method of high-quality amomum villosum seedlings and a planting method of the amomum villosum. The breeding method comprises the following steps: selecting a male parent stolon and a female parent stolon of amomum villosum growing in a same nursery garden, cutting the male parent stolon to obtain a male parent notchand cutting the female parent stolon to obtain a female parent notch, combining the male parent notch and the female parent notch to form a grafting notch, wrapping the grafting notch, and carrying out trimming management after the grafting notch is healed, wherein the trimming management comprises the steps of cutting off the root part of the male parent stolon and cutting off the bud part of thefemale parent stolon, or cutting off the bud part of the male parent stolon and cutting off the root part of the female parent stolon. According to the breeding method, the high-quality male parent stolon and the high-quality female parent stolon are used for cutting grafting on the premise that the male parent stolon and the female parent stolon are not separated from mother plants, and the obtained amomum villosum seedlings also belong to an excellent variety. The grafting success rate is high, and the yield is high. The amomum villosum obtained by using the amomum villosum planting methodprovided by the invention is high in quality and high in yield.

The invention provides special bio-organic fertilizer for lotus root planting. The lotus root is an economical crop with a high nutritive value, however, the lotus root rhizome rot is frequently caused due to continuous cropping and mainly happens to a subterraneous stem and roots of the lotus root, thereby causing yellowing, browning and rotting of leaves of the overground part, even withering of the overground part; the initial symptoms of the subterraneous stem are not remarkable, a vascular bundle in the center can be found browning when cut, the browning part gradually expands to a newly grown subterraneous stem, and rotting and blacking of the subterraneous stem are even caused; the leaves of the overground part then have the symptoms, the leaf color fades, the leaves are dried up and curled, and even leaves of the whole field get withered and yellow as if burnt by fire; the subterraneous stem of the diseased or infected plant has hyphae and pink viscous substances, and part of the stem has water-soaking scab; finally, stolon, diseased stems and diseased root parts all have purple brown scab, the stolon gets brown inside, the diseased stem longitudinally shrinks, and the lotus root necrotizes; and the part firstly infected by the lotus root rhizome rot is in an aquatic environment, and the disease cannot be easily found and is difficult to control.

The invention discloses an apparatus for mist cultivating strawberry stolon seedlings and a mist cultivating method, which relate to the strawberry stolon seedling cultivation and especially relate to an apparatus for mist cultivating strawberry stolon seedlings and mist cultivating method for improving reproduction efficiency of the strawberry stolon seedlings through mist cultivating way. The apparatus for mist cultivating the strawberry stolon seedlings is characterized in that the apparatus comprises an arched shed, a nutrient solution supply device and an arched shed cover device; the arched shed cover device covers on the arched shed; the mist cultivating method comprises: (a). promote strawberry stock plants to generate roots; (b). plant the stock plants; (c). and harvest stolon seedlings. According to the invention, the strawberry stock plants are planted on the top portion of an arched frame; the nutrient solution below the arched frame is sprayed on the stock plant roots in a mist way, thus the roots of the stock plants can be cultivated and grow under the mist environment; and based on the properties of indeterminate growth of the mist cultivated plant roots and by reasonably regulating the nutrient solution, the roots are promoted to grow rapidly, thus improving the seedling cultivating efficiency.

The invention discloses a method for rapid propagation of rauwolfia yunnanensis by tissue culture technology, belonging to the technical field of plant tissue culture. The method is divided into four stages: induction, propagation, root creation and seedling hardening-off. Devilpepper youngshoot with stolon section serves as an explant; after being disinfected, the explant is inoculate to an initiation culture medium to induce auxiliary buds to come out, the composition of the initiation culture medium is MS+2.0-4.0mg / LBA+0.05-0.1mg / LNAA.Enrichment culture is MS+2.5-5.0mg / LBA+0.1-0.5mg / LNAA, multiplication coefficient is 8-12 and passage is repeated every 4-5 weeks. A rooting medium is a 1 / 2MS culture medium in which 1.0mg / LNAA or IBA is added or NAA+IBA=1.0mg / L, after 5-6 weeks, root seedlings are transplanted to a seedling hardening-off ground substance, moisture preservation, overshadowing and seedling hardening-off are carried out for 3-4 weeks at 20-30 DEG C on the root seedlings, then the root seedlings are transplanted or planted in the field with a survival rate of 90%.The method of the invention features simple operation, rapid propagation, good genetic stability, thus providing technological supports for industrialized nursery process.

The invention belongs to the field of environmental ecology and particularly relates to a method for remediating heavy metal cadmium contaminated water by using clonality of a wetland clone plant. The method comprises steps as follows: the clone plant, namely, paspalum paspaloides, is planted on the bank side of the cadmium contaminated water, the tip of the plant is placed in the cadmium contaminated water after being planted, so that the plant grows in a creeping manner and branches on the water surface to form a netted structure; the plant and ramets on the bank side form a connected stolon network system; the paspalum paspaloides plant on the water surface is mowed regularly. With the adoption of the method, under the clone integration action of ramets on the bank side and ramets on the water surface, cadmium ions in the cadmium contaminated water can be removed effectively, the heavy metal cadmium contamination condition of wetland water is relieved, the ecological environment of wetland is improved, the wetland landscape is beautified, and the wetland ecology and service functions are restored and protected effectively.

The invention discloses a water spinach facility cultivating method which aims to treat facility soil salinization. The water spinach facility cultivating method comprises the steps that in a facility such as a conservatory or a greenhouse in which arid crops are conventionally planted and salinization problems occur due to excessive fertilizer application, ridges are built on the periphery of the facility, the inner sides of the ridges are lined with plastic films to prevent moisture side leakage, beds with the width of 1.2 m to 1.5 m are built inside the facility, the depths of furrows range from 20 cm to 30 cm, and high-density direct seeding or high-density seedling transplantation of water spinach (a variety which is a water spinach type, grows vertically and has narrow leaves) is performed on the bed faces. Irrigation is performed according to the growth height of the plants and according to the limit that the plants needing growing are not flooded, water can also be retained in the furrows to keep the bed faces to be sufficiently wetted, and irrigation is performed again if the surface soil of the bed faces becomes white. Surface water or well water with low hardness is used for the irrigation water. Cut harvesting is performed with 5-8 cm stubble in an earlier stage harvesting process, and possible stolons growing transversely are removed in time so that disturbance to the population can be avoided; when the population tends to closing in a later stage, the density of the population is controlled through the height of the stubble and the depth of water flooding.

The invention discloses a breeding method for shortening the productive phase of licorice root seeds. The breeding method includes the steps of land selection and preparation, breeding material selection and treatment, transplanting, fertilization and management, weeding and water and waterlogging prevention, artificial supplementary pollination, growth control and increase of the set of fruit, pest control, overwintering protection and timely seed collecting. According to the method, a licorice root old root which is more than five years old serves as a breeding female parent, a stolon is cut and transplanted, the productive phase of the licorice root seeds is shortened, and the yield and the quality of the licorice root seeds are improved through the methods that the growth environment of licorice roots is improved, young shoots are pinched off and the like.

The invention belongs to plant non-test tube rapid propagation technology, and relates to a rapid propagation method for cajuput seedlings. The method comprises the following steps: A, the selection and pre-treatment of in-vitro materials: selecting stem buds from sprouts on stolons of cajuput seedlings, disinfecting incisions with carbendazim, and soaking in naphthylacetic acid; B, the field planting of the in-vitro materials: inserting the in-vitro materials into a pearlstone substrate; C, the rooting period management of the in-vitro materials: controlling the plant growing environmental condition through an automatic control system, from the beginning of the budding of a new root, spraying a nutrient solution once per 24 hours until the length of the new root is 3-5 cm through visual inspection; D, the hardening-seedling period management of the in-vitro materials: entering the nursery stock period after detecting that 1-2 leaves are grown at the upper parts of the in-vitro materials; E, the nursery stock period management: transplanting into a cultivation container or a cultivation ground bed having the conventional cajuput cultivation soil, so that commercial seedlings can be obtained after 8-10 days. According to the invention, the propagation speed and propagation survival rate are greatly improved, and the rapid propagation method is more suitable for the industrial implementation of large-scale rapid seedling culture.

The invention discloses a strawberry cutting seedling raising method. The strawberry cutting seedling raising method comprises the following steps: 1) selecting stolon seedlings with 2-3 expanded leaves; 2) soaking the lower ends of the stolon seedlings in fresh water for 10-12h and then soaking the lower ends of the stolon seedlings in growth regulating agent for 10-12h to obtain adventitious roots; 3) trimming a seedbed, punching holes in a ridge surface at distance with plant spacing of 20cm according to the standard of two rows per ridge, then inserting the adventitious roots in the holes, performing watering and keeping the ground temperature to be 10-20DEG C; 4) firstly erecting a sunshade net on the seedbed, simultaneously performing fertilization treatment, and keeping the temperature to be 20-25DEG C, illuminating time to be 10-12h and water holdup to be 70-80 percent. The strawberry cutting seedling raising method disclosed by the invention has the advantages that the steps are simple, the operation is convenient to perform, the cost is low, the performance of stock strawberries is effectively guaranteed to be excellent, the characteristics of short cultivation period, good rooting effect, quick seedling growth and high survival rate are realized, and the practice and popularization significance is great.

The invention provides an efficient propagation method for stolon-type landscape ground cover plants (single-section stem-segment broadcasting). The method is concretely based on the characteristic that stem nodes of landscape plants can produce adventitious roots and lateral buds and utilizes the characteristic to effectively improve propagation efficiency thereof. The method comprises the following steps: (1) moderately leveling and sufficiently watering a propagation site; (2) putting to-be-propagated stolon maternal plants in order, aligning nodes of every stolon in turn to the greatest extent and then cutting the stolons in batches into stem segments having a singe node; (3) moderately cutting off leaves on single-node stem-segment nodes; (4) uniformly broadcasting single-node stem segments on the propagation site, covering the single-node stem segments with soil about 5 mm thick and moderately watering; and (5) performing spray irrigation quantificationally at regular time or covering with plastic film to keep soil-surface humidity and promote the growth of sprouts of the broadcast stem segments and performing normal management when the sprouts produce 1 to 2 leaves. The method has the advantages of saving a great amount of manpower and money and significantly improving the propagation efficiency of stolon-type landscape ground cover plants, along with high propagation coefficient.

A method for picking, storing and planting the root and stem of lawn includes such steps as picking the root and stem of lawn with stolon by special root-stem picking machine, storing them while regulating the atmosphere in storing room, uniformly spreading them on the ground, covering by soil, irrigating, rolling and pruning. Said special root-stem picking machine is composed of a running main body, a power unit, and several rotary knife holder with curved knives installed at intervals.

The invention belongs to the plant non-test-tube rapid breeding technology, and relates to a rapid breeding method for grape seedlings. The method comprises the following steps: A. selecting and pre-treating an ex-vivo material: selecting stem buds from sprouts on stolons of the grape seedlings, disinfecting the notch through carbendazim, and soaking through naphthylacetic acid; B. permanently planting the ex-vivo material: inserting the ex-vivo material into a pearlstone substrate; C. performing the rooting period management of the ex-vivo material: controlling the plant growth environment condition through an automatic control system; if new roots begin to bud, spraying a nutrient solution once per 24 hours, and obtaining the length of the new root is 3 to 5 cm through visual-estimating; D. performing seedling refining period management of the ex-vivo material: detecting that 1 to 2 pieces of blades are grown at the upper part of the ex-vivo material, and then ensuring the ex-vivo material to enter a nursery stock period; E. performing nursery stock period management: transplanting the ex-vivo material into a culture container or a culture ground bed with conventional grape culture soil, and obtaining commodity seedlings after 18 to 25 days growing. The method greatly improves the breeding speed and breeding survival rate, and is suitable for industrialized implementation of large scale rapid breeding better.

The invention discloses a method for the in situ remediation of eutrophic surface water, which comprises the following steps: 1) The pre-culture of plants is that after the jussiaea stipulacea ohwi collected from fields is purified, the jussiaea stipulacea ohwi is cultivated and domesticated in a green house for nine to eleven days by using the hoagland nutrient solution, which is in accord with the international standard; 2) The sewage treatment is that the cultivated and domesticated jussiaea stipulacea ohwi is transplanted to the surface of the governance water area, and the excessive nutrient salt of nitrogen and phosphor in the water is absorbed by the stolon and root system of the jussiaea stipulacea ohwi; the jussiaea stipulacea ohwi with the fresh weight of five to twenty kilograms is placed on the governance water area per cubic metre. The method of the invention can realize the biological in situ remediation of eutrophic surface water.

The invention discloses a method for promoting side slope green retrieval through mycorrhiza inoculation. Firstly, stolons of plants undergo cuttage in a matrix mixed with arbuscular mycorrhizal fungi, and watering and maintenance are performed for propagation expanding; the plants obtained through the propagation expanding are taken out, the stolons are sheared into stem sections with adventitious roots and the matrix; fermentation broth of ectotrophic mycorrhiza, side slope plant seeds and an organic fertilizer for sterilization are taken and mixed to obtain a mixture; a base material is spray-seeded on a side slope; the obtained plant stem sections and the mixture are mixed and spray-seeded; arbor seedlings are transplanted, the ectotrophic mycorrhiza and / or the arbuscular mycorrhizal fungi are taken to wrap broken roots of the arbor seedlings or the ectotrophic mycorrhiza and / or the arbuscular mycorrhizal fungi is scattered to pits, the arbor seedlings are planted in the pits; the stem sections spray-seeded with a bacterium carrying agent can be used for inoculating the arbuscular mycorrhizal fungi to all the side slope plants, the side slope plants and the spray-seeded stolon section plants grow rapidly, the demand for rapid green retrieval at the earlier stage of side slope green retrieval can be met, mycorrhiza fungi inoculation is specifically conducted on the arbor seedlings, and the demand for keeping vegetation for a long period of the side slope can be met.

The invention relates to a strawberry planting method for promoting flowering, and belongs to the technical field of agriculture. The strawberry planting method for promoting the flowering is provided to solve the problem that in the prior art, the planting time is too long as planting needs seed seedling raising. The strawberry planting method comprises the following steps that a strawberry plant or a strawberry stolon cultivating plant in a quiescent phase is cultivated, and sterilization is conducted; cold treatment, enhancement treatment and normal cultivation are conducted, and then a strawberry is promoted to flower. According to the strawberry planting method, no seed breeding method is required, a strawberry flower bud is effectively promoted to form, and the effect of promoting the strawberry to flower is achieved. Only 5-8 weeks are required to promote the strawberry to enter a flowering phase, the cultivation period of the strawberry is highly reduced, the flowering rate of the strawberry can reach more than 92%, and the fruiting rate of obtaining fruits at last can reach more than 90%.

The invention provides a rapid propagation method for acquiring virus-free strawberry seedlings. The method comprises the following steps: (1) selecting a raw material and sterilizing the raw material; (2) bud culture: removing sepals of a sterilized bud, then inoculating the bud into a prepared culture medium of MS+6-BA1.0mg / L+NAA0.1mg / l for culture till clumpy buds grow out; (3) propagation culture: transferring the clumpy buds onto a culture medium of MS+6-BA0.1mg / L for propagation culture; (4) induced rooting: cutting off strawberry seedlings from the clumpy buds, transferring the strawberry seedlings to the culture medium of 1 / 2MS+NAA0.1mg / L culture medium, inducing the rooting, and transferring the rest of callus to the culture medium of MS+6-BA0.1mg / L for culture; (5) seedling hardening: transplanting the rooted strawberry seedlings into a culture plate from a culture bottle, and carrying out the seedling hardening growth in a greenhouse. By utilizing the method, the method for acquiring the virus-free seedlings is simple, the acquired seedlings are strong, stolon reproduce quickly, and the quality is good.

The invention provides a method of increasing the caulerpa lentillifera commodity output rate and improving the caulerpa lentillifera commodity quality. The method includes the steps that in an initial stage of culture, upright stems of caulerpa lentillifera are subjected to cut-off and low-light suspension culture to be induced to generate stolons; in a floating bed culture stage, a gradual addition control strategy is conducted on light intensity and nutrient salt; in middle and later stages of culture, the upright stems are cut off to keep appropriate culture density. The method of increasing the caulerpa lentillifera commodity output rate and improving the caulerpa lentillifera commodity quality is designed according to different light intensity and nutrient salt which are acquired during induced generation of different caulerpa lentillifera body parts in different stages of caulerpa lentillifera culture. By adopting the method of increasing the caulerpa lentillifera commodity output rate and improving the caulerpa lentillifera commodity quality, it can be promoted that the upright stems of caulerpa lentillifera generate plenty of stolons in the initial stage of growth, the caulerpa lentillifera body growth speed in the floating bed culture stage and the commodity output rate are significantly increased, and upright stem products with high quality are obtained in a harveststage.