Application of streptomyces murinus JKTJ-3 in strawberry seedling culture

Abstract

The invention discloses application of streptomyces murinus JKTJ-3 in strawberry seedling culture, which is characterized in that the streptomyces murinus JKTJ-3 is used for preparing a streptomyces murinus JKTJ-3 microbial inoculum with the streptomyces spore number of 10 < 9 > cfu. G <-1 >-10 < 12 > cfu. G <-1 >, and the obtained streptomyces murinus JKTJ-3 microbial inoculum is applied to strawberry seedling culture. The streptomyces murinus JKTJ-3 microbial inoculum can significantly increase the plant height, stem diameter, root length, leaf number, biomass and the like of strawberry stock plant seedlings, and in the later strawberry stock plant transplanting propagation period, the stock plant cultivated by using the streptomyces murinus JKTJ-3 microbial inoculum has the advantages that the stolon growing time is advanced, and the growing number and the seedling number are significantly increased. Therefore, the streptomyces murinus JKTJ-3 is applied to strawberry mother plant seedling cultivation, the effect of improving the strawberry mother seedling breeding efficiency is remarkable, and the blank of a microorganism method in strawberry seedling cultivation is filled.

Description

Technical Field

[0001] The invention belongs to the technical field of strawberry seedling cultivation, and particularly relates to application of Streptomyces muridarum JKTJ-3 in strawberry seedling cultivation. Background Art

[0002] Since the 1950s, fertilizers and growth regulators have played an important role in agricultural production. However, the widespread use of these traditional agricultural inputs has led to a series of problems such as soil pollution, ecological imbalance and environmental degradation. Therefore, the development of more environmentally friendly and sustainable agricultural solutions has become the research focus of scientists from all over the world. In recent years, microbial agents and microbial fertilizers with microorganisms as the core have gradually become new green inputs and have received more and more attention.

[0003] Streptomyces is a type of higher actinomycetes. Due to its large spore production and long spore survival time, it is easy to make living cell preparations, including spore powder, seed coating, mycelium culture, etc., and is used in agricultural production. Compared with other microorganisms, Streptomyces has the ability to produce rich and diverse bioactive substances. It can secrete plant growth regulators such as auxin or gibberellin, effectively promote plant growth, and become a high-quality plant growth-promoting bacteria source. Studies have shown that Streptomyces can effectively inhibit the growth of pathogenic fungi by secreting extraspore chitinase and glucanase. For example, S. plicatus 101 inhibits the growth of some pathogenic fungi hyphae by secreting extracellular chitinase, thereby indirectly promoting plant growth.

[0004] In addition, Streptomyces can secrete siderophores through nutritional competition, which can enhance the plant's absorption of iron ions in the soil, while limiting the competition for iron ions by pathogens, thereby reducing the occurrence of diseases. It has been reported that Streptomyces can increase the dry weight of plants such as corn, cucumbers, and tomatoes by secreting growth hormones. For example, S. hygroscopicus can produce a variety of hormone analogs to promote plant growth. Streptomyces can promote plant growth by increasing plant mineral nutrient absorption and organic matter synthesis. For example, Streptomyces chiba can increase the nitrogen, phosphorus, and potassium content in broad beans and promote protein accumulation. S. lydicus can promote the growth of tomatoes, increase the number of leaves, enhance photosynthesis, and increase the content of plant growth regulators such as abscisic acid and deionized salicylic acid in tomatoes.

[0005] In strawberry production, the application of Streptomyces is mainly focused on disease prevention and control, such as the prevention and control of strawberry root rot by Streptomyces nigromaculata and the prevention and control of strawberry gray mold by Streptomyces nigromaculata. In addition, some studies have shown that Streptomyces can improve the quality of strawberries. For example, Streptomyces hygroscopicus can increase the Vc content and sugar-acid ratio in the fruit. However, high-quality strawberry seedlings are the key link in the green, high-quality and efficient production of strawberry cultivation. At present, there is little research and application on the use of Streptomyces to promote the growth of strawberry mother plants and improve the reproduction coefficient of strawberries.

[0006] At present, strawberry seedling cultivation mainly promotes the growth of strawberry mother plant seedlings by applying fertilizers, humic acid and plant growth regulators, as well as increasing the number of runners and seedlings produced by strawberry mother seedlings. However, research and reports on the use of microorganisms, especially Streptomyces, to promote strawberry seedling cultivation (runner propagation) are still very scarce. Therefore, it is necessary to further study the application potential of Streptomyces in strawberry seedling cultivation to achieve innovation in strawberry seedling cultivation technology. Summary of the invention

[0007] Based on the above-mentioned prior art, the present invention provides the application of Streptomyces muridarum JKTJ-3 in strawberry seedling cultivation. The present invention finds for the first time that the application of Streptomyces muridarum JKTJ-3 in strawberry seedling cultivation can effectively promote the expansion and propagation of strawberry runners and improve the reproduction coefficient of strawberry mother seedlings.

[0008] The technical solution adopted to achieve the above-mentioned purpose of the present invention is:

[0009] Application of Streptomyces muridarum JKTJ-3 in strawberry seedling cultivation (promoting the propagation of strawberry runners).

[0010] A Streptomyces muridarum JKTJ-3 bacterial agent, whose effective ingredients are Streptomyces muridarum JKTJ-3 and its fermentation products.

[0011] Further, the number of spores of Streptomyces muridarum JKTJ-3 in the bacterial agent is 10 9 cfu·g -1 ~10 12 cfu·g -1 .

[0012] The invention discloses an application of a Streptomyces muridarum JKTJ-3 bacterial agent in strawberry seedling cultivation (promoting the propagation of strawberry runners).

[0013] Compared with the prior art, the beneficial effects and advantages of the present invention are:

[0014] The present invention uses Streptomyces muridarum JKTJ-3 to prepare Streptomyces spores with a number of up to 10 9 cfu·g -1 ~10 12 cfu·g -1The Streptomyces muridaris JKTJ-3 bacterial agent is applied to strawberry seedling cultivation, which can significantly increase the plant height, stem thickness, root length, leaf number, biomass, etc. of strawberry mother plant seedlings, and in the subsequent strawberry mother plant transplanting and expansion period, the mother plant cultivated with the Streptomyces muridaris JKTJ-3 bacterial agent has an early generation time of runners, and the number of generation and the number of seedlings are significantly increased. It can be seen that the present invention applies Streptomyces muridaris JKTJ-3 to the cultivation of strawberry mother plant seedlings, and has a significant effect in improving the reproduction efficiency of strawberry mother seedlings, filling the gap in the microbial method in strawberry seedling cultivation. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Actual photos of strawberry mother plant seedlings cultivated in different seedling media.

[0016] Figure 2 These are actual photos of strawberry mother plants propagating seedlings using different seedling media when the runners begin to develop.

[0017] Figure 3 Actual photos of strawberry mother plants propagated from different propagation media when runners begin to develop. DETAILED DESCRIPTION

[0018] The present invention is described in detail below with reference to specific embodiments.

[0019] Unless otherwise specified, the sources of the raw materials used in the following examples are as follows:

[0020] Bacteria: Streptomyces murinus (S.murinus) JKTJ-3 (Mihong Ge, Xiang Cai, Dehuan Wang, et al. Efficacy of Streptomyces murinus JKTJ-3 in Suppression of Pythium Damping-Off of Watermelon. Microorganisms, 2023, 11, 1360.).

[0021] Fermentation raw materials: peat and bran, purchased from the market.

[0022] Example 1

[0023] 1. Inoculate S.murinus JKTJ-3 on a plate with ISP-2 medium, streak evenly, and culture in a 28°C incubator for 5 days until the spores are full to obtain an activated strain plate. Use a bacterial block puncher to punch holes in the activated strain plate to obtain an activated bacterial block with a diameter of 8 mm.

[0024] 2. Put peat and bran into a 250 mL conical flask in a mass ratio of 4:6. The total mass of coconut bran and bran (solid matrix) is 20 g. Add 24 g of water (material-water ratio is 1:1.2) to the conical flask and stir evenly.

[0025] 3. Add 0.9 g of carbon source glycerol (the mass of mannitol is 3% of the total mass of the solid matrix) to the conical flask, stir evenly, and then sterilize at 121°C for 40 min to obtain the solid fermentation medium of Streptomyces muridarum JKTJ-3 (referred to as medium JKTJ-3).

[0026] 4. Inoculate three activated bacterial blocks on the culture medium JKTJ-3, then stir every 24 hours in a constant temperature incubator at 30°C. After 7 days of fermentation, obtain the JKTJ-3 bacterial agent of Streptomyces muridarum (referred to as bacterial agent JKTJ-3). Determine the number of spores produced by the JKTJ-3 strain of Streptomyces muridarum (referred to as strain JKTJ-3) in the bacterial agent JKTJ-3 by the dilution plate method. Repeat the experiment 3 times, and calculate the average number of spores produced by strain JKTJ-3 in the bacterial agent JKTJ-3. After measurement and calculation, the average number of spores of strain JKTJ-3 is 7.73×10 11 cfu·g -1 .

[0027] Example 1

[0028] 1. Common matrix (matrix CK, mixed by peat and perlite in a volume ratio of 3:1) is used as a control group strawberry mother plant seedlings seedlings matrix, and then the bacterial agent JKTJ-3 prepared by Example 2 is added to the common matrix (the ratio of bacterial agent JKTJ-3 to the common matrix is ​​3g / L) and mixed, and seedling matrix JKTJ-3 (referred to as matrix JKTJ-3) is made as the experimental group strawberry mother plant seedlings seedlings matrix. Matrix JKTJ-3 and matrix CK are respectively loaded into disposable seedling pots, strawberry runner seedlings are cut, and cut into seedling pots, and managed according to conventional mother seedlings seedling method.

[0029] 2. After 70 days of cultivation, the following measurements were performed on the strawberry mother plant seedlings in the experimental group and the control group:

[0030] 1) Take photos of strawberry seedlings grown on different seedling substrates. Figure 1 shown.

[0031] 2) The biomass indices such as plant height, stem thickness, root length and leaf number of strawberry mother plant seedlings cultivated in different seedling cultivation media were measured. The biomass indices of strawberry mother plant seedlings cultivated in different seedling cultivation media are shown in Table 2 below:

[0032] Table 1 Effects of different seedling culture media on the biomass of strawberry mother plant seedlings

[0033]

[0034] From Table 2 and Figure 1 It can be seen that compared with the control group, the biomass indicators such as plant height, stem thickness, root length and leaf number of the strawberry mother plant seedlings in the experimental group were significantly increased, which shows that the bacterial agent JKTJ-3 of the present invention has a good growth-promoting effect on strawberry mother plants.

[0035] 3) The nutrient contents of P, K, Ca and Mg in strawberry mother plant seedlings cultivated with different seedling cultivation media were measured. The nutrient contents of the elements in strawberry mother plant seedlings cultivated with different seedling cultivation media are shown in Table 2 below:

[0036] Table 2 Effects of different seedling culture media on the nutrient content of strawberry mother plant seedlings

[0037]

[0038] As shown in Table 3, the matrix JKTJ-3 can promote the absorption and accumulation of nutrients in strawberry mother plant seedlings. The P, K, Ca and Mg nutrient contents of the strawberry mother plant seedlings in the experimental group were significantly higher than those in the control group, especially the P content increased by 63.1% compared with the control.

[0039] 4) The plant hormone contents of IAA, GA, ABA, and ETH and the activities of SOD, POD, and CAT resistance-related enzymes in strawberry mother plant seedlings cultivated with different seedling cultivation substrates were measured. The plant hormone contents and resistance-related enzyme activities in strawberry mother plant seedlings cultivated with different seedling cultivation substrates are shown in Table 4 below:

[0040] Table 3 Effects of different seedling culture media on hormones and resistance-related enzyme activities in strawberry mother plant seedlings

[0041]

[0042] As shown in Table 3, compared with the control group, the IAA, GA, ABA, ETH plant hormone contents and the activities of SOD, POD, CAT resistance-related enzymes in the experimental group of strawberry seedlings (cultivated with seedling medium JKTJ-3) were significantly increased.

[0043] 3. Place common substrate (mixed with peat and perlite in a volume ratio of 3:1) into two groups of planting bags respectively, set the strawberry mother seedlings of the experimental group obtained in step 2 as the strawberry mother plants of the experimental group in one group of planting bags, and set the strawberry mother plant seedlings of the control group obtained in step 2 as the strawberry mother plants of the control group in another group of planting bags. When the runners of the strawberry mother plants of the control group begin to occur and end, perform the following measurements:

[0044] 1) When 25% of the strawberry mother plants in the control group began to form runners, the strawberry mother plants cultivated with different seedling media were photographed. The results are as follows: Figure 2 shown.

[0045] 2) When 25% of the strawberry mother plants in the control group began to grow runners, the reproduction indicators such as the number of runners and the number of seedlings of the strawberry mother plants cultivated with different seedling mediums were measured. The reproduction indicators of the strawberry mother plants cultivated with different propagation mediums are shown in Table 4 below:

[0046] 3) When the runners of 75% of the strawberry mother plants in the control group were completed, the reproduction indicators of the strawberry mother plants cultivated with different seedling substrates were statistically shown in Table 4 below:

[0047] Table 4 Effects of different seedling medium on strawberry mother plant propagation

[0048]

[0049] Depend on Figure 2 As shown in Table 4, the strawberry mother plants cultivated with the seedling medium JKTJ-3 showed early occurrence of runners and fast seedling formation. At the same time, the number of runners and seedlings at different stages were significantly higher than those of the control group, indicating that the seedling medium has a significant promoting effect on the reproduction of strawberry seedlings.

[0050] Example 2

[0051] 1. Use ordinary matrix (mixed with peat and perlite in a volume ratio of 3:1) as the matrix for raising strawberry mother plant seedlings. Put the ordinary matrix into a disposable seedling pot, cut the strawberry runner seedlings, and cut them into the seedling pot. Manage them according to the conventional mother seedling raising method.

[0052] 2. After 70 days of cultivation, strawberry mother plant seedlings are obtained.

[0053] 3. Use common matrix (matrix CK, which is composed of peat and perlite mixed in a volume ratio of 3:1) as the control group strawberry mother plant propagation matrix, add the bacterial agent JKTJ-3 prepared in Example 2 to the common matrix (the ratio of bacterial agent JKTJ-3 to the common matrix is ​​1g / L) and mix to make a propagation matrix JKTJ-3 (referred to as matrix JKTJ-3) as the experimental group strawberry mother plant propagation matrix, put the matrix CK into one group of planting bags, put the matrix JKTJ-3 into another group of planting bags, and plant the strawberry mother plant seedlings obtained in step 2 as strawberry mother plants in the planting bags containing the matrix JKTJ-3 (experimental group), and plant the strawberry mother plant seedlings obtained in step 2 as strawberry mother plants in the planting bags with the matrix CK (control group). When the runners of the strawberry mother plants in the control group begin to occur and when the occurrence ends, the following measurements are performed:

[0054] 1) When 25% of the strawberry mother plants in the control group began to produce runners, the strawberry mother plants cultivated with different propagation media were photographed. The results are as follows: Figure 3 shown.

[0055] 2) When 25% of the strawberry mother plants in the control group began to grow runners, the reproduction indicators such as the number of runners and the number of seedlings of the strawberry mother plants cultivated with different propagation substrates were measured. The reproduction indicators of the strawberry mother plants cultivated with different propagation substrates are shown in Table 6 below:

[0056] 3) When the runners of 75% of the strawberry mother plants in the control group were completed, the reproduction coefficients of the strawberry mother plants cultivated with different propagation media were statistically analyzed and shown in Table 5 below:

[0057] Table 5 Effects of different propagation media on the propagation of strawberry mother plants

[0058]

[0059] right Figure 3 By analyzing the data in Table 5, we can draw the following conclusions: Strawberry mother plants cultivated using the propagation matrix JKTJ-3 showed earlier runner formation and rapid seedling growth. In addition, at different growth stages, the number of runners and seedlings was significantly higher than that of the control group, indicating that the propagation matrix has a significant promoting effect on the reproduction of strawberries.

Claims

1. Application of Streptomyces muridarum JKTJ-3 in strawberry seedling cultivation.

2. A Streptomyces muridarum JKTJ-3 bacterial agent, characterized in that: The effective ingredients are the Streptomyces muridarum JKTJ-3 and its fermentation products as described in claim 1.

3. The Streptomyces muridarum JKTJ-3 bacterial agent according to claim 2, characterized in that: The number of spores of Streptomyces muridarum JKTJ-3 in the bacterial agent is 10 9 cfu·g -1 ~10 12 cfu·g -1 .

4. An application of the Streptomyces muridarum JKTJ-3 inoculant according to claim 2 in strawberry seedling cultivation.

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