583 results about "Corm" patented technology

The present invention relates to a method for controlling pathogen growth on live plants and mushrooms using UV-C light and an apparatus for use in the method. Also provided are methods for removing surplus leaves and methods for destroying aerial plant parts prior to harvest of underground roots, tubers or bulbs.

This invention relates to human adipose tissue-derived multipotent adult stem cells. More particularly, the invention relates to human adipose tissue-derived multipotent stem cells, which can be maintained in an undifferentiated state for a long period of time by forming spheres and have high proliferation rates, as well as methods for isolating and maintaining the adult stem cells, and methods for differentiating the multipotent adult stem cells into nerve cells, fat cells, cartilage cells, osteogenic cells and insulin-releasing pancreatic beta-cells. Also, the invention relates to cellular therapeutic agents for treating osteoarthritis, osteoporosis and diabetes and for forming breast tissue, which contain the differentiated cells or the adult stem cells. Although the multipotent stem cells are adult stem cells, they have the ability to differentiate into osteogenic cells, nerve cells, astrocytes, fat cells, chrondrogenic cells or insulin-releasing pancreatic beta-cells, and so are effective in treating osteoporosis, osteoarthritis, nerve disease, diabetes, etc. Also, the stem cells form spheres in a serum-free medium containing CORM-2, and thus can be maintained in an undifferentiated state for a long period of time. Also, the stem cells have very high proliferation rates. Accordingly, the stem cells are useful as cellular therapeutic agents.

The invention belongs to the biotechnology field and relates to a hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings. The hormone-free cultivation and rapid propagation method comprises the steps: germinating dendrobium candidum seeds in an axenic mode; forming and preserving protocorms and seedlings; rooting and proliferating unrooted seedlings, cultivating sound seedlings; and toughening and transplanting the axenic seedlings. The technology has convenient operation, small occupied space and low production cost, is convenient to industrially produce and apply on a large scale and is beneficial to retaining the excellent property of the seedlings and increase the use safety of the seedlings when being used for propagating the dendrobium candidum.

The invention relates to a method for cultivating algam Dendrobium nobile, wherein it comprises that putting the seed of algam Dendrobium nobile into 1/2MS basic medium with NNA (a- fruitone), banana juice and agar; then transplanting the former corm into 1/2MS basic medium with BA (6- benzyl adenine), banana juice and agar, to cultivate small seed into middle and large seeds; then planting the large seed into the hole of wall.The invention can improve the yield and confirm the better penetration and enough light of large seed.

The invention discloses a rhizoma bletillae industrialized seedling method. According to the method, the advantage of large quantity of rhizoma bletillae capsule seeds is fully utilized; with the seeds as an explant, a rhizoma bletillae tissue culture rapid breeding technology and a tissue culture seedling domestication technology are established; germination and growth are carried out under an aseptic condition, so that the influence on external environment and germination of rhizoma bletillae seeds is overcome, and the germination rate and the growth speed of the rhizoma bletillae seeds are greatly increased. By only needing 5 months, the seeds can grow into plants of which the corm diameter is about 0.7cm, and only one-time grafting is needed in the process, so that the industrialized production of the rhizoma bletillae seeds is realized; moreover, the method is easy to operate, low in cost and high in reliability; the whole seedling period is about 8 months, and the domestication survival rate is 91 percent or above; the rhizoma bletillae seedlings which are domesticated for about 3 months are directly transplanted into a field so that the survival rate is up to about 98 percent and the rhizoma bletillae can be harvested after 4-5 years. The problem that the rhizoma bletillae seedlings are difficult to breed is solved, and the industrialized seedling of the rhizoma bletillae is realized.

The invention discloses a production method of bletilla seed stems, which comprises the following steps: 1) explant treatment and inoculating, 2) protocorm obtaining and multiplication, 3) differentiation and seedling forming of protocorms, 4) test-tube corm obtaining, 5) primary seed stem obtaining and 6) secondary seed stem obtaining. According to the method, mature bletilla seeds are subjected to aseptic seeding and culture, many test-tube seed stems are obtained, and the larger production seed stems capable of being grown in a large field are obtained by primary and secondary seed stem reproduction. The method solves the problem of a low survival rate of direct exercising of tissue culture seedlings, provides a seed source for manual massive bletilla planting, and lays a foundation for sustainable development, exploitation and utilization of bletilla.

The invention relates to a method for planting saffron crocus in a Tibet region and belongs to the technical field of natural medicine plantation. The method comprises the steps of field seed ball breeding and indoor cultivation flower picking. The field seed ball breeding comprises the sub-steps of selection of seed corms, plantation preparation, plantation, field management, harvesting of the seed corms and storage of the seed corms. The indoor cultivation flower picking comprises the sub-steps of loading into flat baskets, shelving, cultivation management, and harvesting and processing of the saffron crocus. The saffron crocus is planted in a large field in the Tibet region by using the plantation method, sunlight is sufficient, the plantation time of the seed corms in the land is long, flowers are picked in the land, and the cost is reduced; by virtue of the selection and the cooperation of various plantation parameters according to the technical scheme, filaments of the produced saffron crocus are long, the appearance is good, and the yield is high; the content of saffron crocus essence and saffron crocus bitter principle is greatly higher than that of the saffron crocus produced in the prior art, the medicinal value is high, and pesticide residues are eliminated; moreover, seed balls are large, low in lesion and low in degeneration.

The invention discloses a dendrobium officinale sprout rapid propagation method with high efficiency. The method comprises the following steps: a) selecting dendrobium officinale shoot tip or aseptic seedling as explants, performing induction and propagation culture of protocorm like bodies; b) performing differentiation and seeding formation on protocorm like bodies; c) culturing strong plantlet and rootage of seedling grown thickly; and d) hardening seedling and transplanting of the aseptic seedling. According to the invention, shoot tip and aseptic seedling are taken as the explants, thereby the problem that stem bud and top bud as the explants to induce the protocorm like bodies, the explant source is limited can be solved, the mature protocorm like bodies can be rapidly and continuously obtained with large amount, the incidence rate of the protocorm like bodies can reach as high as 99%, the seedling differentiation coefficient can reach as high as 50 strains/0.1g protocorm like bodies, the period for acquiring the seedling is 4 months, the seedling is tidy, the rooting rate is 100%, the root length is about 5cm, and the root number can reach 10 strips/crowd, the transplanting survival rate can reach as high as more than 95%, and the purposes of shortening the seedling period, increasing the propagation coefficient and unlimited germplasm source can be reached. The rapid propagation method can rapidly produce large batch of sprouts, and is suitable for large scale production.

The invention aims at providing a method for efficiently and quickly producing a bletilla striata seedling. The method comprises (1) a liquid culture stage and (2) a solid medium culture stage, wherein (1) the liquid culture stage comprises the particular steps that a bletilla striata capsule is disinfected via sodium hypochlorite; the capsule is cut; a seed is sown in a liquid medium for culture; the seed is developed into a protocorm; (2) the solid medium culture stage comprises the particular steps that the protocorm is induced to be differentiated into a root-free seedling, and then transferred to a rooting culture medium for rooting culture, and a cultivation substrate transplanted into a greenhouse is domesticated before field transplanting. The method realizes efficient and quick production of the bletilla striata seedling; compared with whole-course solid culture, the method overcomes the defect that the seed germination rate is low when an early seed is cultured on a solid medium; the seed germination rate is increased significantly and reaches 99%; the fresh weight and the dry weight of the protocorm are increased greatly; and the whole production cycle is shortened significantly, and can be shortened to about 110 days.

A method for breeding seed konjac from konjac corms through segmenting with a lateral bud is disclosed. The specific method and the benefits thereof are as follows: the terminal bud of each konjac corm before overwintering storage is removed so as to start and promote the physiological activity of lateral buds in the overwintering period; after overwintering, the konjac corm is segmented so that the dominant lateral buds of the corm are more obvious; after transverse cutting of the corm, the corm is vertically cut so that the upper half part of the corm is divided into four pieces, thereby ensuring that each piece is provided with the dominant lateral bud and also ensuring that the weight of each piece is between 50 g and 80 g; therefore, the expansion coefficient of each segment is increased when enough nutrients for survival are present; before seeding, dormancy breaking and germination acceleration are performed so as to germinate the segments in advance; as a result, the growth period is prolonged; in addition, diseased seeds can be discovered before seeding to avoid losses; an appropriate cultivation method is capable of accelerating the konjac to sprout and come up out of the ground as soon as possible; a konjac dedicated callusing chemical and a seed konjac dedicated segmenting cutter are used, thereby avoiding wound infection; and a systematic breeding method through segmenting with the lateral bud is capable of increasing the survival rate and the expansion coefficient and improving the economic benefit.

The invention discloses a factory production method of a dendrobium officinale seedling, which comprises the steps of explant selection, pretreatment, sterilization, lateral bud germination, protocorm inducement, subculture, protocorm differentiation culture, strong seedling rooting culture, and bottle seedling transplant, and a specially-prepared culture medium is used in each step for culturing. The method has the benefits that an explant is easy to take; the sterilization efficiency is high; the growth coefficient is high and stable; components of the culture media are unique, concise and practical; the reproductive cycle is short; the production cost is low; and the produced seedling is stable in hereditary character, strong and tidy, and strong in growth vigor.

A tissue culture and fast reproduction method of azalea orchid includes such steps as aseptic sowing and culturing of azalea seeds to grow protocorm, or disinfecting and inoculating the bud eye of pseudocorm to obtain protocorm, culturing in the culture medium for reproducing by 3-7 times, differentiating to become seedlings, promoting their rooting to obtain regenerated plants, transplanting in vermiculite bed for transition or directly in vermiculite for rooting and germinating, transplanting in soil and later-phase management.

The invention discloses a cultivating method of Japanese Kyushu saffron after being introduced to China. The method is obtained on the basis of researching the concrete seeding process, the fertilizing method and the like of the saffron on the basis of an existing saffron-cultivating technology, so that the yield and the quality of the saffron can be improved. The cultivating method mainly comprises the steps of land selection, fertilization of sufficient basic fertilizers, corm preparation, seeding, field management, timely harvesting, shelving, lateral-bud removing, timely picking and collection and the like. The seed ball of the cultivated Japanese Kyushu saffron is not easily degraded, the obtained saffron is bright in color, filaments are thick and strong, and all indexes meet the requirements of related standards.

The invention discloses a domestication method of a rhizoma bletillae tissue culture corm. The method comprises the following steps of: cultivating the rhizoma bletillae tissue culture corm from rhizoma bletillae seeds by an aseptic germination and tissue cultivation mode in a tissue culture room; acclimatizating and transplanting the cultivated rhizoma bletillae tissue culture corm by a domestication substrate, wherein the components of the domestication substrate are decomposed sawdust, vermiculite and broken bark; the mass ratio of the sawdust to the vermiculite to the broken bark is 3:1:1; and the domestication time is more than one year; acclimatizating and transplanting for 3-6 months by the domestication substrate after 2-3 new tubers grow from a tiller of the rhizoma bletillae tissue culture corm to carry out field production as germchit (seed ball). By adopting the domestication method, the rhizoma bletillae tissue culture corm is acclimatized and transplanted by the domestication substrate, and then the new tubers growing from the tiller are subjected to field production as the germchit after being acclimatized for a period of time. By adopting the method, the survival rate of transplanting the rhizoma bletillae tissue culture seeding into the field can be effectively improved; the method is simple; and the adopted domestication substrate is low in cost and easy to obtain.

A planting module for planting a seed or a bulb in the earth includes the seed or bulb disposed in a dissoluble capsule so that after the module has been placed in the earth and water is supplied, the capsule disintegrates and the seed or bulb sprouts.

The invention discloses a method for obtaining germination effective symbiotic fungus of Cymbidium bicolor seeds, which includes: using dead twigs, fallen leaves, bark, moss, humus and the like around an original habitant of Cymbidium bicolor as culture medium, subjecting the culture medium and the seeds of Cymbidium bicolor to ex-site symbiotic germination to induce the seeds to germinate and generate protocorms; sterilizing the surface of each obtained protocorm, culturing with thy artificial culture medium under the sterile condition, inducing endophytic fungus in the protocorms to grow, purifying the fungus to obtain a pure colony after mycelia appears; and subjecting the obtained fungus and the seeds of Cymbidium bicolor to symbiotic germination culturing, carrying out a control experiment to check the effectiveness of the obtained fungus to germination of the seeds of Cymbidium bicolor, screening germination effective symbiotic fungus for the seeds of Cymbidium bicolor, and subjecting the fungus to molecular identification and storage. The method is simple to operate, singe variety of fungi can be obtained, massive and troublesome screening is not needed, and a new way of using Cymbidium bicolor seeds and fungus to symbiotically germinate to culture seedlings is opened.

The invention discloses a method for quickly recovering scirpus planiculmis wet lands by utilizing underground nutrition bulbs, which relates to a method for recovering the scirpus planiculmis wet lands. The method aims to solve the problem that stop lands and food for white cranes are gradually reduced in the biotope of scirpus planiculmis due to heavy damage. The method for quickly recovering the scirpus planiculmis wet lands by utilizing the underground nutrition bulbs comprises the steps of (1) investigation of collection lands, (2) investigation of recovery land, (3) seed source collection, (4) hydrological recovery and (5) sowing and management, so as to complete the method for quickly recovering the scirpus planiculmis wet lands by utilizing the underground nutrition bulbs. The method is applied to quickly recovering the scirpus planiculmis wet lands by utilizing the underground nutrition bulbs.

The invention relates to a saffron crocus planting method. The saffron crocus planting method comprises the steps of outdoor corm breeding: saffron crocus corm can be transplanted in an outdoor field in last November and can be removed from soil in next May; indoor bloom cultivation: from the middle of May to mid-to-end of June, seedballs are in a complete dormancy period, and the room temperature needs to be up to 30 DEG C and needs to be maintained for more than 20 days; from the end of June to the last ten days of July, the room temperature needs to be maintained in 23-28 DEG C, the relative humidity is 80%, the indoor environment is in sun shading, and the illumination is avoided; from August to the middle of September, the room temperature needs to be maintained in 23-27 DEG C, the relative humidity is 75%-85%, the indoor environment is in sun shading, and the illumination is avoided; from the end of the middle of September to the last ten days of October, white parts are presented by main sprouts of the corm, the room temperature is maintained in 20-25 DEG C, and the relative humidity is 80%; from the end of October to the middle of November, the room temperature is 15-18 DEG C, the humidity is 75%, and the indoor environment is not in sun shading any more. According to the saffron crocus planting method disclosed by the invention, the yield is high, the quality is good, and the economic benefit is good.

A test tube technique for reproducing the konjak features that the paclobutrazol is added to the culture medium MS+NAA5-1 mg/L used for rooting the tissue culture seedlings of konjak, they are cultured at temp higher than 20 deg.C, under light radiation (1000 LX) for 20-25 days to form small bulb, when the weight of small bulb reeches 2-4 g they are cultured at lower than 15 deg.C in dark environment, after cultured in flask for 10-15 day they are sprouted and then transplanted in seedbed, and they are cultured at higher than 20 deg.C, while the fertilizer is proportionally and periodically applied.

The invention relates to the field of flower propagation methods, in particular to the field of corm flower propagation methods. A thin layer culture method of tulips comprises the following steps of: longitudinally or transversely cutting tulip scales into thin layers of 0.1-5mm, and laying the thin layers on a culture medium; and inducing and proliferating the culture medium into an MS (Murashige and Skoog) culture medium, wherein 1.0-2.0mg of 6-BA (Benzyl Aminopurine), 0.1-1.0mg of NAA (Naphthyl Acetic Acid), 1.0-4.0mg of 2, 4-D (2,4-Dichlorophenoxyacetic acid), 20g of cane sugar and 6g of agaragar are added to the MS culture medium per L, the culture condition is that the temperature is 23+/- 2 DEG C, the illumination intensity is 1,500-2,000Lx, and the illumination time is for 16h/d. The thin layer culture method of the tulips has the advantages and active effects: the tissue culture bulb inductivity reaches 100%, induced tissue culture seedlings grow vigorously, the source of the tissue culture seedlings is not limited by seasons, and the tissue culture scales are the better source of thin layer culture materials. Cutting modes cause different inductions on bulbs, and the differentiation rate and the bulb induction number of the scales which are transversely cut are higher than those of the scales which are longitudinally cut.