Method for rapid tissue propagation and breeding seedling of bletilla striata seeds

Abstract

The invention provides a method for rapid tissue propagation and breeding seedling of bletilla striata seeds. The method comprises the following steps: disinfection and sterilization of the seeds, seed germination and protocorm induction, protocorm propagation, cluster bud induction and propagation, rooting culture, seedling adaptation and transplantation. The method has the benefits that the seed tissue culture is adopted, and obtained seedlings are low in pollution rate, high in differentiation, and high in content of active substances; meanwhile, through the adoption of the method, the tissue culture and propagation period of bletilla striata is greatly shortened, and the tissue culture period from seeds to rooted seedlings is only about 4 months, so that the production time is greatly shortened, and the production cost is reduced.

Description

technical field

[0001] The invention relates to the field of traditional Chinese medicine planting, in particular to a method for rapid propagation of bletilla striata seed tissue. Background technique

[0002] Bletilla striata is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae. It is also used as bletilla striata, Lianji grass, and sweet root. It is distributed from China to Korea and Japan. It is produced in East China, South China, Henan, Shaanxi, Sichuan, Yunnan and other places in China. The tuber of bletilla striata is a traditional Chinese medicine in my country. It has been recorded in "Shen Nong's Materia Medica" as early as in "Chinese Pharmacopoeia". Hemoptysis, bleeding from golden sores, painful ulcers, burns from soup and fire, chapped hands and feet, etc. In addition, bletilla striata has bright colors and is also a good landscape plant, so the market demand for bletilla striata is very strong. Due to the expansion of mark...

Examples

Embodiment 1

[0024] (1) Preparation and sterilization of Bletilla striata seeds: Take uncracked and robust Bletilla striata capsules, wash them with washing powder for 1-2 minutes, rinse them with running water, and soak them in 70% ethanol for 30s on an ultra-clean workbench , and then soaked in 0.15% mercuric chloride for 20 minutes, rinsed with sterile water for 4-5 times, put it on sterilized absorbent paper, and blot the water for later use.

[0025] (2) Seed germination and protocorm induction: After the seeds are sterilized, they can be inoculated on the protocorm induction medium, and the temperature is controlled at 22-24°C during the day and 18-20°C at night, in a dark box Carry out seed cultivation, after 5-6 days, the seeds begin to expand and increase. At this time, adjust the cultivation conditions, the temperature is controlled at 25±2°C, the light intensity is 1500-2000Lx, and the light is 12 hours a day. Protocorms are formed after 10 days.

[0026] (3) Protocorm prolifera...

Embodiment 2

[0031] (1) Preparation and sterilization of Bletilla striata seeds: Take uncracked and robust Bletilla striata capsules, wash them with washing powder for 1-2 minutes, rinse them with running water, and soak them in 70% ethanol for 30s on an ultra-clean workbench , and then soaked in 0.15% mercuric chloride for 20 minutes, rinsed with sterile water for 4-5 times, put it on sterilized absorbent paper, and blot the water for later use.

[0032] (2) Seed germination and protocorm induction: After the seeds are sterilized, they can be inoculated on the protocorm induction medium, and the temperature is controlled at 22-24°C during the day and 18-20°C at night, in a dark box Carry out seed cultivation, after 5-6 days, the seeds begin to expand and increase. At this time, adjust the cultivation conditions, the temperature is controlled at 25±2°C, the light intensity is 1500-2000Lx, and the light is 12 hours a day. Protocorms are formed after 10 days.

[0033] (3) Protocorm prolifera...

technical field

[0001] The invention relates to the field of traditional Chinese medicine planting, in particular to a method for rapid propagation of bletilla striata seed tissue. Background technique

[0002] Bletilla striata is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae. It is also used as bletilla striata, Lianji grass, and sweet root. It is distributed from China to Korea and Japan. It is produced in East China, South China, Henan, Shaanxi, Sichuan, Yunnan and other places in China. The tuber of bletilla striata is a traditional Chinese medicine in my country. It has been recorded in "Shen Nong's Materia Medica" as early as in "Chinese Pharmacopoeia". Hemoptysis, bleeding from golden sores, painful ulcers, burns from soup and fire, chapped hands and feet, etc. In addition, bletilla striata has bright colors and is also a good landscape plant, so the market demand for bletilla striata is very strong. Due to the expansion of mark...

Examples

Embodiment 1

[0024] (1) Preparation and sterilization of Bletilla striata seeds: Take uncracked and robust Bletilla striata capsules, wash them with washing powder for 1-2 minutes, rinse them with running water, and soak them in 70% ethanol for 30s on an ultra-clean workbench , and then soaked in 0.15% mercuric chloride for 20 minutes, rinsed with sterile water for 4-5 times, put it on sterilized absorbent paper, and blot the water for later use.

[0025] (2) Seed germination and protocorm induction: After the seeds are sterilized, they can be inoculated on the protocorm induction medium, and the temperature is controlled at 22-24°C during the day and 18-20°C at night, in a dark box Carry out seed cultivation, after 5-6 days, the seeds begin to expand and increase. At this time, adjust the cultivation conditions, the temperature is controlled at 25±2°C, the light intensity is 1500-2000Lx, and the light is 12 hours a day. Protocorms are formed after 10 days.

[0026] (3) Protocorm prolifera...

Embodiment 2

[0031] (1) Preparation and sterilization of Bletilla striata seeds: Take uncracked and robust Bletilla striata capsules, wash them with washing powder for 1-2 minutes, rinse them with running water, and soak them in 70% ethanol for 30s on an ultra-clean workbench , and then soaked in 0.15% mercuric chloride for 20 minutes, rinsed with sterile water for 4-5 times, put it on sterilized absorbent paper, and blot the water for later use.

[0032] (2) Seed germination and protocorm induction: After the seeds are sterilized, they can be inoculated on the protocorm induction medium, and the temperature is controlled at 22-24°C during the day and 18-20°C at night, in a dark box Carry out seed cultivation, after 5-6 days, the seeds begin to expand and increase. At this time, adjust the cultivation conditions, the temperature is controlled at 25±2°C, the light intensity is 1500-2000Lx, and the light is 12 hours a day. Protocorms are formed after 10 days.

[0033] (3) Protocorm prolifera...