Domestication method of rhizoma bletillae tissue culture corm

A technology for tissue culture balls and bulbs, applied in the field of agricultural science and technology, can solve the problems of low income, low yield and high input cost, and achieve the effects of improving survival rate, good use effect and simple method.

Active Publication Date: 2013-07-31
GUIZHOU CROP VARIETIES RESOURCE INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Carrying out artificial cultivation is the most effective way to solve the market demand and protect the resources of Baiji. At present, Baiji is planted as a bulb, and the input cost is high. Because the cultivation technology is quite immature, the yield is low, the income is small or even loss, so that enterprises all over the country Or the actual planting area of ​​farmers is general

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  • Domestication method of rhizoma bletillae tissue culture corm
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  • Domestication method of rhizoma bletillae tissue culture corm

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Embodiment Construction

[0023] Embodiments of the present invention: a method for acclimating bletilla tissue culture bulbs, utilizing the aseptic germination tissue culture of bletilla seeds, and cultivating tissue culture bulbs in a tissue culture room. Wash the culture medium of the tissue culture bulbs, sample them by quartering, weigh them separately, use the cluster analysis method (analysis software: DPSv7.05) to classify the bulbs, and select white and tissue culture bulbs with a single bulb not less than 0.5g , the selected white and tissue culture bulbs are used to domesticate the seedlings with domestication substrates. The components of the domestication substrates are decomposed sawdust, vermiculite and broken bark, and the mass ratio of sawdust, vermiculite and broken bark is 3 : 1:1, the sawdust is the sawdust of Fagaceae trees mixed with pine trees; the broken bark is the broken bark of Fagaceae trees mixed with pine trees, the particle size of the broken barks is less than 1cm; the do...

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Abstract

The invention discloses a domestication method of a rhizoma bletillae tissue culture corm. The method comprises the following steps of: cultivating the rhizoma bletillae tissue culture corm from rhizoma bletillae seeds by an aseptic germination and tissue cultivation mode in a tissue culture room; acclimatizating and transplanting the cultivated rhizoma bletillae tissue culture corm by a domestication substrate, wherein the components of the domestication substrate are decomposed sawdust, vermiculite and broken bark; the mass ratio of the sawdust to the vermiculite to the broken bark is 3:1:1; and the domestication time is more than one year; acclimatizating and transplanting for 3-6 months by the domestication substrate after 2-3 new tubers grow from a tiller of the rhizoma bletillae tissue culture corm to carry out field production as germchit (seed ball). By adopting the domestication method, the rhizoma bletillae tissue culture corm is acclimatized and transplanted by the domestication substrate, and then the new tubers growing from the tiller are subjected to field production as the germchit after being acclimatized for a period of time. By adopting the method, the survival rate of transplanting the rhizoma bletillae tissue culture seeding into the field can be effectively improved; the method is simple; and the adopted domestication substrate is low in cost and easy to obtain.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a method for acclimating white and tissue-cultured bulbs. Background technique [0002] Rare and valuable medicinal material Baiji is the dried bulb of Orchidaceae Baiji. White and ( Bletilla striata ) is a small perennial herb. Due to its high economic value, the market demand is increasing year by year, and its price is rising year by year. Wild resources are destructively excavated. Wild resources are endangered. They are listed as rare and endangered plants in the country for protection. It is also included in Appendix II of the Convention on International Trade (CITES), which is protected internationally and prohibits the trade of wild resources. In addition, Baiji has large flowers and bright colors, and has high ornamental value. Baiji bulbs contain Baiji gum (phlegm), starch, volatile oil, glucose, etc. Baiji tastes bitter, sweet, and astringent; mil...

Claims

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Application Information

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IPC IPC(8): A01G31/00
Inventor 吴明开杨琳毛堂芬陈娅娅王沁李娟田紫萍
Owner GUIZHOU CROP VARIETIES RESOURCE INST
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