Domestication method of rhizoma bletillae tissue culture corm

Abstract

The invention discloses a domestication method of a rhizoma bletillae tissue culture corm. The method comprises the following steps of: cultivating the rhizoma bletillae tissue culture corm from rhizoma bletillae seeds by an aseptic germination and tissue cultivation mode in a tissue culture room; acclimatizating and transplanting the cultivated rhizoma bletillae tissue culture corm by a domestication substrate, wherein the components of the domestication substrate are decomposed sawdust, vermiculite and broken bark; the mass ratio of the sawdust to the vermiculite to the broken bark is 3:1:1; and the domestication time is more than one year; acclimatizating and transplanting for 3-6 months by the domestication substrate after 2-3 new tubers grow from a tiller of the rhizoma bletillae tissue culture corm to carry out field production as germchit (seed ball). By adopting the domestication method, the rhizoma bletillae tissue culture corm is acclimatized and transplanted by the domestication substrate, and then the new tubers growing from the tiller are subjected to field production as the germchit after being acclimatized for a period of time. By adopting the method, the survival rate of transplanting the rhizoma bletillae tissue culture seeding into the field can be effectively improved; the method is simple; and the adopted domestication substrate is low in cost and easy to obtain.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a method for acclimating white and tissue-cultured bulbs. Background technique [0002] Rare and valuable medicinal material Baiji is the dried bulb of Orchidaceae Baiji. White and ( Bletilla striata ) is a small perennial herb. Due to its high economic value, the market demand is increasing year by year, and its price is rising year by year. Wild resources are destructively excavated. Wild resources are endangered. They are listed as rare and endangered plants in the country for protection. It is also included in Appendix II of the Convention on International Trade (CITES), which is protected internationally and prohibits the trade of wild resources. In addition, Baiji has large flowers and bright colors, and has high ornamental value. Baiji bulbs contain Baiji gum (phlegm), starch, volatile oil, glucose, etc. Baiji tastes bitter, sweet, and astringent; mil...

AI Technical Summary

Problems solved by technology

[0003] Carrying out artificial cultivation is the most effective way to solve the market demand and protect the resources of Baiji. At present, Baiji is planted as a bulb, and the input cost is high. Because the cultivation technology is quite immature, the yield is low, the income is small or even loss, so that enterprises all over the country Or the actual planting area of ​​farmers is generally less than 20 mu

Although a large number of seedlings can be obtained by group-cultivating seedlings by aseptic germination of Bletilla seeds, there are no successful case reports on the technology of transplanting to the field, so it is far from alleviating the endangered status of Bletilla resources, which seriously restricts the industrialization of rare medicinal materials Bletilla. develop

Images