75 results about "BLETILLA STRIATA TUBER" patented technology

The invention discloses a domestication method of a rhizoma bletillae tissue culture corm. The method comprises the following steps of: cultivating the rhizoma bletillae tissue culture corm from rhizoma bletillae seeds by an aseptic germination and tissue cultivation mode in a tissue culture room; acclimatizating and transplanting the cultivated rhizoma bletillae tissue culture corm by a domestication substrate, wherein the components of the domestication substrate are decomposed sawdust, vermiculite and broken bark; the mass ratio of the sawdust to the vermiculite to the broken bark is 3:1:1; and the domestication time is more than one year; acclimatizating and transplanting for 3-6 months by the domestication substrate after 2-3 new tubers grow from a tiller of the rhizoma bletillae tissue culture corm to carry out field production as germchit (seed ball). By adopting the domestication method, the rhizoma bletillae tissue culture corm is acclimatized and transplanted by the domestication substrate, and then the new tubers growing from the tiller are subjected to field production as the germchit after being acclimatized for a period of time. By adopting the method, the survival rate of transplanting the rhizoma bletillae tissue culture seeding into the field can be effectively improved; the method is simple; and the adopted domestication substrate is low in cost and easy to obtain.

The invention relates to the distillation and producing method of a kind of amylase material used for accelerating cell adhesive growth. The invention gets natural medicament bletilla striata tuber to have amylase component elementary distillation, eliminate albumen through small molecule hydrophobic substances, then through ion exchange chromatogram and gelatin filtration chromatogram to get pure distilled object bletilla striata amylase material, which is solid and dusty, then have antiseptic treatment. As a kind of amylase, the bletilla striata amylase got from separation and distillation of the natural medicament bletilla striata in the invention can affect with the extracellular matrix (ECM) components, and accelerate cell adhesive and cell wall adnate growth; meantime, the membrane cross-linked by bletilla striata amylase has good molding, strong tenacity, good biology compatibility and degeneration, so it's good choice in tissue engineerig material. It has solves the disadvantage which present technology can't overcome, that amylase substance has strong hydrophilicity and hard plasticity. The preparation method is simple, easy to control and low-cost.

The invention discloses a composition with anti-wrinkling and skin brightening effects, a mask and a preparation method of the mask. The composition is prepared from, by weight, 8-30 parts of aloe barbadensis superfine powder, 6-26 parts of aloe barbadensis leaf juice and 20-42 parts of fresh bletilla striata tuber extract. The bletilla striata anti-wrinkling and skin brightening mask is composed of traditional Chinese medicine effective components and auxiliary materials capable of being accepted in the mask field and has the anti-wrinkling and skin brightening effects, the formula is simple and effective, and no irritation and anaphylaxis are generated to the skin; the fresh bletilla striata tuber extract and the aloe barbadensis leaf juice are greatly combined by mixing effective components and dissolved to generate more effective substances; and according to main components of bletilla striata, cell activity improving, anti-wrinkling, skin whitening and skin brightening are achieved, fresh aloe barbadensis is adopted, moistening, nourishing, anti-wrinkling and whitening are achieved, and the effective components of the bletilla striata are effectively enhanced.

The invention discloses a cultivation method for bletilla striata seed stems. The bletilla striata seed stems which can be directly sowed to the field are cultivated by the domestication of bletilla striata tissue culture tubers for 4-6 months, effective, high-yield and high-quality seedlings (seed stems) are provided for artificial cultivation of bletilla striata, common farmer households can directly sow the bletilla striata seed stems to the field, the yield per mu of fresh products of bletilla striata medicinal materials can reach 1,000-1,500kg after 3-4 years according to a standardized planting technical regulation, and the output value per mu is 100,000-150,000 RMB according to a current price. According to the method, the bottleneck problem of restriction of a large-scale bletilla striata production core technology is solved, the bletilla striata can be truly and artificially produced, rare medicinal resources are protected, and raw material guarantee is provided for industrialization development of the bletilla striata.

The invention discloses a breeding method of rhizoma bletillae seedlings, and belongs to the technical field of traditional Chinese medicinal material plantation. According to the breeding method, tissue culture propagation is adopted. The breeding method comprises following steps: 1, seedling collecting; 2, culture medium selecting; 3, seedling breeding, wherein seed germination induction, induced bud differentiation, rooting induction, and greenhouse seedling hardening are adopted; 4, bottle seedling emergence; 5, removing of bottle seedlings out from bottles and processing; 6, domesticated plant cultivation management; and 7, domesticated plat emergence. Compared with the prior art, the breeding method possesses following advantages: a defect of propagation by tuber that seed consumption amount is large is avoided; rhizoma bletillae tissue culture plant survival rate is increased; rhizoma bletillae seedling domestication cultivation measures are provided, and domesticated plant survival rate is increased; rhizoma bletillae culture medium formula ratio, bottle seedling, and domesticated plant seedling emergence standards are standardized, and lack of technology and standards in large-scale production is avoided effectively.

The invention provides a formula of a mask capable of whitening skin and removing freckles. The formula comprises the following components: radix ginseng, bighead atractylodes rhizome, radix angelicaedahurioae, peach blossom, radix notoginseng, safflower, pearl, radix ampelopsis, Chinese angelica, peach kernels, aconitum fischeri peichb, rhizoma chuanxiong, poria cocos, bletilla striata tuber, radix salviae miltiorrhizae and almonds. The formula particularly comprises the following raw material medicines in parts by weight: 350-500g of radix ginseng, 800-1000g of bighead atractylodes rhizome,800-1000g of radix angelicae dahurioae, 500-800g of peach blossom, 500-800g of radix notoginseng, 250-300g of safflower, 1000-1300g of pearl, 800-1000g of radix ampelopsis, 250-400g of Chinese angelica, 500-600g of peach kernels, 1000-1200g of aconitum fischeri peichb, 500-600g of rhizoma chuanxiong, 500-600g of poria cocos, 500-600g of bletilla striata tuber, 1000-1200g of radix salviae miltiorrhizae and 500-600g of almonds. The formula conforms to the differentiation theory of traditional Chinese medicine in selection and compatibility of traditional Chinese medicinal materials, has obvioustreatment effects, is free of toxic and side effects, and achieves the purpose of supplementing one other in traditional Chinese medicine matching.

The invention discloses a processing method of a common bletilla tuber crude medicine. The processing method of the common bletilla tuber crude medicine comprises the following steps: harvesting common bletilla tubers; washing the harvested common bletilla tubers so as to have soil on the surface of the common bletilla tubers rinsed off; putting the washed common bletilla tubers into warm water, and adding baijiu of which the ethanol concentration is 52%vol at a warm water to ethanol volume-ratio of 1000 to 3 so as to be subjected to soaking; taking the soaked common bletilla tubers out, air-drying the common bletilla tubers, and removing fibrous roots and peel; slicing the dried common bletilla tubers, namely curing the common bletilla tubers without the fibrous roots and the peel, air-cooling the common bletilla tubers and carrying out slicing so as to obtain common bletilla tuber slices; crushing the common bletilla tuber slices, namely drying the common bletilla tuber slices by using hot air, and carrying out particle pulverization so as to obtain particle-pulverized common bletilla tuber powder; carrying out separation and extraction, namely adding purified water into the particle-pulverized common bletilla tuber powder, carrying out uniform stirring, carrying out ultrasonic treatment for 30 minutes, carrying out heating and extracting, separating residues from liquid after completion of the extraction, and repeatability extracting the residues for more than 3 times so as to obtain extracted liquid materials; concentrating the extract, namely mixing the liquid materials from the various times of extractions so as to obtain a mixture, concentrating the mixture so as to obtain a concentrate, hydrolyzing the concentrate so as to obtain a hydrolysate, performing decolorizing treatment on the hydrolysate, and carrying out centrifugal separation so as to obtain a liquid supernatant; and then, carrying out vacuum freeze-drying, namely freezing the liquid supernatant so as to have moisture sublimated, and carrying out high-pressure sterilization so as to obtain common bletilla tuber powder. The processing method of the common bletilla tuber crude medicine is capable of preserving gum components in common bletilla tubers to the maximum extent; and thus, the prepared common bletilla tuber crude medicine is high in biological activity and high in purity.

The invention discloses bletilla glucomannan self-assembled nano particles, and a preparation method and applications thereof. According to the preparation method, bletilla glucomannan dry powder with a molecular weight ranging from 20 to 150kDa is extracted from bletilla striata tuber, and the bletilla glucomannan self-assembled nano particles are obtained via a plurality of steps. According to the bletilla glucomannan self-assembled nano particles, the porosity, the gelling capacity, and the stability of bletilla glucomannan are maintained and improved; no toxic or side effect is caused; the bletilla glucomannan self-assembled nano particles possess excellent biocompatibility, is capable of stopping bleeding, promoting wound healing, and stopping overflow of exudate, and can be used for tissue haemostasis, tissue sealing, and tissue bonding in surgical fields such as general surgery department, orthopedics department, cardiothoracic surgery department, neurosurgery department, and gynaecology and obstetrics.

The invention discloses a method for planting gastrodia elata by utilizing artificial mushroom-sticks, and relates to the technical field of planting of traditional Chinese medicinal herbs. By utilizing that the vigorous growth period of armillaria mella is about equal to the growth period of germination of gastrodia elata seeds into protocorms, the method provided by the invention improves the inoculation rate and the survival rate of the gastrodia elata seeds; the artificial mushroom-sticks used in planting are made of waste wood chips or branches, so broad-leaved trees are protected from uncontrolled cutting; meanwhile, the upper and lower layers of humus soil respectively undergo modification treatment according to different nutrient requirements of the gastrodia elata seeds and the armillaria mella, so nutritional value and heat-preserving and cold-resistant effects of the humus soil are improved, and the rot rate of the gastrodia elata is reduced. The method provided by the invention has the following advantages: gastrodia elata planted by using the method provided by the invention has large tubers and high effective ingredient content; market values and economic benefits aregreatly improved; a novel way is provided for planting of the gastrodia elata; and the method is applicable to popularization of gastrodia elata planting.

The invention discloses gastrodia elata macromolecule linear straight-chain glucan and a preparation method and application thereof, and belongs to the field of traditional Chinese medicine. The method comprises the steps: drying gastrodia elata tubers, smashing and degreasing with ethyl alcohol; extracting at room temperature, centrifuging and concentrating an extracting solution, carrying out alcohol precipitation, precipitating and redissolving, dialyzing by adopting a dialysis bag with the molecular weight cutoff of 8000-14000 Da, concentrating the dialyzed solution, and further separatingand purifying by using ion exchange resin and gel exclusion chromatography to obtain the gastrodia elata macromolecular linear straight-chain glucan. The polysaccharide is only composed of glucose, is alpha-D-1, 4 connected homopolydextran, and is a new dextran. When the concentration is 400 [mu] g/mL, macrophage proliferation can be significantly promoted, the phagocytic ability of macrophages can be enhanced, the macrophages can be promoted to release NO and cytokines TNF-alpha and IL-6, and the body immunity can be enhanced.

The invention discloses bletilla striata oligosaccharide extracted from bletilla striata rhizomes and a preparation method of the bletilla striata oligosaccharide. The method comprises the following steps: 1) extraction: slicing bletilla striata tubers, adding deionized water for multiple times of extraction, combining extracting solutions, and performing centrifugal filtration to obtain a bletilla striata tuber extracting solution; 2) alumina column treatment: treating the bletilla striata tuber extracting solution with a neutral alumina column to obtain an effluent A; 3) cross-linked dextrangel column treatment: concentrating the effluent A, loading the concentrated effluent A to a treated cross-linked dextran gel column for adsorption separation, eluting with deionized water, and collecting eluent to obtain effluent B; and 4) drying: concentrating and freeze-drying the effluent B to obtain the bletilla striata oligosaccharide. The bletilla striata polysaccharide is decolorized anddeodorized through the neutral aluminum oxide and the cross-linked dextran gel column, polysaccharide and free proteins are separated, no obvious peculiar smell is generated after the obtained bletilla striata oligosaccharide is dissolved, no toxic or harmful reagent is added in the extraction process, and the production criterion of health and environmental protection is met.

The present invention discloses a preparation method and a use of a 2-isobutyl malate glucooxybenzyl ester extract in rhizoma bletillae and belongs to the fields of medicine and daily chemical industry. The rhizoma bletillae extract is preferably tuber and/or fibrous root. The rhizoma bletillae 2-isobutyl malate glucooxybenzyl ester extract can be used as a tyrosinase inhibitor in preparation of cosmetics, pharmaceuticals or skin external preparations of melanin production inhibitors or skin-whitening agents, and has good prevention, improvement and treatment effects on skin pigmentation of skin tanning, stain, age spots, freckles, etc. after ultraviolet radiation.

The invention discloses a treatment method for preventing soft rot disease of the tuber of pinellia ternate and belongs to the technical field of cultivation of traditional Chinese medicinal materials. The key point of the method is that a lot of seed stems are disinfected and sterilized before the seed stems of pinellia ternate are planted to prevent the soft rot disease so as to improve the planting yield of pinellia ternate and the quality of the tuber. The method mainly comprises the following steps: grading; primarily airing seeds; selecting seeds; sterilizing; immersing seeds; secondarily airing seeds; sterilizing the surface of the tuber of pinellia ternate; and then immersing seeds. The soft rot disease and spread of pathogen can be prevented, so that the planting yield and officinal quality of pinellia ternate can be improved. The method is simple and feasible, convenient to use and suitable for being popularized and applied to wide farmer households of pinellia ternate.

The invention discloses an ice clara-lemon raw fish capable of clearing away heart-fire. The ice clara-lemon raw fish is prepared from the following raw materials in parts by weight: 92-100 parts of frozen sailfish, 15-20 parts of lyophilized royal jelly powder, 3-6 parts of red wine, 8-10 parts of lemon pulp, 4-5 parts of broadleaf holly leaves, 14-18 parts of beef, 14-15 parts of yunwu (Chinese character) fruits, 8-10 parts of pumpkin seeds, 1.1-1.5 parts of ice clara, 1.5-2.3 parts of canna edulis ker tubers, 1.8-2.1 parts of lavender, 0.2-0.5 part of sodium glutamate, 0.1-0.2 part of phosphate, 1-2 parts of lactic acid bacteria, 300mmol/L of CaCl2 solution, and a proper amount of sesbania gum, papain, refined salt and sugar. The ice clara-lemon raw fish has the advantages of unique taste, long shelf life and good health effect; the ice clara and lemon raw fish is frozen by a pickling method, and is pickled by an injection method; the technology is unique and novel; the value-added space and the extension benefits of the raw fish product are increased; and due to the addition of traditional Chinese medicine components such as ice clara and canna edulis ker tubers, walnut kernel is endowed with the health effects of tonifying the stomach, promoting digestion, promoting the secretion of body fluid to relieve thirst, enriching the blood and tonifying qi.

The invention provides a dark energy-saving in-vitro propagation method for micro tubers of polygonatum cyrtonema and belongs to the technical field of plant tissue culture. According to the method, the apical dominance of the explant is reduced by optimizing a plant growth regulator and carrying out in-vitro culture on the polygonatum kingianum explant, the growth potential of lateral buds is released from a dormant state, and tuber formation is induced. Cytology observation finds that the rosette type plant explant can form a plurality of tubers, and each tuber is derived from an axillary bud at the base of a leaf. Unlike adventitious organs, newly developed tubers originate from intrinsic lateral buds, and no callus is formed. The tuber production system of the present invention provides a new method for propagating polygonatum cyrtonema plants, facilitating easier storage, transplantation and tuber seeding. Under the condition that no callus is formed, higher hereditary stability can be obtained.

The invention discloses a preparation method of bletilla striata polysaccharide, which comprises the following steps: (1) pulverizing: pulverizing bletilla striata tubers to obtain bletilla striata coarse powder; (2) ultrasonic treatment: adding a proper amount of water into the bletilla striata coarse powder prepared in the step (1), and carrying out ultrasonic extraction to obtain an ultrasonictreatment liquid; (3) enzymolysis treatment: adding cellulase into the ultrasonic treatment liquid prepared in the step (2) for treatment, and centrifuging after treatment to obtain a bletilla striatapolysaccharide extract liquid; and (4) microfiltration and concentration: carrying out tangential flow filtration on the bletilla striata polysaccharide extract liquid prepared in the step (3) by using a ceramic membrane filter, and collecting microfiltration trapped fluid with required concentration to obtain colloidal liquid bletilla striata polysaccharide. The preparation method can be used for efficiently extracting bletilla striata polysaccharide in an environment-friendly manner and also can be used for effectively improving the extraction purity.

The invention discloses a breeding method of rhizoma polygonati in the technical field of cultivation of traditional Chinese medicinal materials. Wild rhizoma polygonati is collected and dug, multiplecandidate varieties are mixed and cultivated under the same site condition, and then one or more resistant environments such as drought, waterlogging, weed damage and disease and pest damage are simulated or created for primary resistance screening; the roots and stems of the selected varieties are sterilized first, and then the parts with buds at the ends are selected as a provenance, after seedlings raising, a field comparison test is carried out, after traditional steaming and drying of tubers, in combination with the determination of effective ingredients total saponins and polysaccharidecontent, the rhizomes with the total saponins content of active ingredient indexes of 12 mg/g or above or the polysaccharide content of 14 mg/g or above are selected as mother species and cultivatedcontinuously for 2-4 years, and the total saponins content and polysaccharide content of the cultivated rhizomes are 90% or higher of the effective ingredient indexes; and then a one-year evaluation planting test is carried out to select the variety with the highest total saponin content and polysaccharide content after addition to obtain a breeding variety with the highest effective medicinal ingredients.

The invention discloses application of mixed nitrogen in promoting growth of rhizoma bletillae and improving photosynthetic efficiency and polysaccharide accumulation, relates to the related field of plant growth, and aims to obtain the effect of promoting the growth of rhizoma bletillae by the mixed nitrogen by researching different nitrogen forms on the growth of rhizoma bletillae, chlorophyll fluorescence characteristics and polysaccharide accumulation. The accumulation of dry matters is facilitated, the photosynthetic efficiency of rhizoma bletillae can be improved, and the accumulation of polysaccharide is facilitated. The total nitrogen concentration of the mixed nitrogen is 15 mmol/L, NO3--N: NH4 < + >-N in the mixed nitrogen is 1: 1, Ca (NO3) 2.4 H2O and KNO3 are used for NO3--N, (NH4) 2SO4 is used for NH4 < + >-N, the mixed nitrogen promotes the rhizoma bletillae to grow leaves, increase the plant height and increase the stem diameter, dry matter accumulation of the overground part and the tuber part is improved, the mixed nitrogen promotes the rhizoma bletillae to grow under the weak light condition, and the mixed nitrogen improves the maximum photochemical efficiency of the leaves and the episightseeing synthetic electron transfer rate, and promotes polysaccharide accumulation.

The invention discloses an automatic integrated peeling device for tuber traditional Chinese medicine treatment, and relates to the field of machinery. The automatic integrated peeling device comprises a base, wherein the upper side of the base is provided with a driving bevel gear, and the rear side of the driving bevel gear is cooperatively provided with a rotating bevel gear; the rear end of the rotating bevel gear is fixedly connected with a rotating shaft, a peeling box is slidably mounted on the front side of the rotating shaft, a mounting plate is fixedly mounted on the rear side of thepeeling box, a rotating shaft is fixedly mounted on the rotating shaft in the peeling box, and a sliding sleeve is slidably mounted on the rear side of the rotating shaft; rotating wheels are fixedlymounted on the outer sides of the sliding sleeves, and rotating wheels are cooperatively mounted on the outer sides of the rotating wheels; the front side of the rotating wheel is fixedly connected with a fixing rod. A device cover is slidably installed outside the sliding sleeve. The rear side of the device cover is fixedly connected with a moving frame and the rear end of the moving frame is fixedly connected with moving rods. The device is reasonable in design, movable feeding and rotary peeling can be conducted on tuber type traditional Chinese medicine, and cutters can be replaced according to the sizes of tubers, thereby improving the working efficiency of the device.