238 results about "Soluble solids" patented technology

The invention discloses a diffuse transmission spectrum nondestructive testing method and a portable device for large thick-peel melons and fruits, belongs to the field of nondestructive testing of fruit quality, and provides a real-time and portable internal quality nondestructive testing method and device suitable for the large thick-peel melons and fruits. The system comprises a camera obscura, a shading sucker, an annular shading sponge, a light source module, a spectrum detection module, a collimating lens, an optical fiber, a Raspberry Pi controller and a touch screen. During working, the halogen light source emits a focused light beam to penetrate through melons and fruits, the focused light beam is converted into an electric signal by the spectrum detection module after being scattered and transmitted, and the Raspberry Pi analyzes in real time to obtain and display indexes such as hardness, pH, soluble solids and the like. A 220V industrial power supply is adopted to drive a high-power light source, a cooling fan and a shading sucker structure are combined to reduce environmental interference, and an annular shading sponge ensures that a light path is closed. An integrated portable device of a high-power light source is realized, and the device can be used for on-site rapid nondestructive detection of key quality indexes of large thick-peel melons and fruits such as Hami melons and the like.

The invention discloses a hyperspectral prediction method and device for the soluble solid content of winter jujubes and a storage medium, and belongs to the field of material testing and analys.The method comprises the steps that firstly, a winter jujube sample is obtained, and spectral data and soluble solid content data of the winter jujube sample are measured; preprocessing the spectral data by using a preset data preprocessing method to obtain enhanced spectral data, constructing an input sample set through the obtained enhanced spectral data, and constructing an output sample set through the measured soluble solid content data; then, the input sample set and the output sample set are used for training a pre-configured LSTM-Transformer network, and the pre-configured LSTM-Transformer network is used for training the pre-configured LSTM-Transformer network; and finally, deploying the trained LSTM-Transform network as a winter jujube SSC hyperspectral prediction model so as to predict the soluble solid content of the winter jujube. Through the steps, the soluble solid content of the winter jujube sample can be quickly and non-destructively predicted with high precision, so that the consistency and the stability of the product quality are ensured.

The invention provides application of molybdenum disulfide nanoparticles in preparation of a tomato leaf fertilizer, and particularly relates to the technical field of tomato fertilizers. The molybdenum disulfide nano-particles act on tomato plants in a foliage spraying manner, so that the growth of the tomato plants is promoted and / or the fruit quality is improved. The molybdenum disulfide nanoparticles are used as the foliar fertilizer to significantly increase the plant height and stem diameter of tomatoes, increase the chlorophyll content and trace element content of plants, increase the vitamin C content, soluble sugar content and soluble solid content of tomato fruits, and significantly increase the accumulation of Mo and S of plants through the sulfur-molybdenum synergistic effect, so that the yield of the tomatoes is increased. A scientific basis is provided for reasonable application of the nano molybdenum fertilizer, and a way is opened up for high-yield and high-quality cultivation of tomatoes.

The invention belongs to the technical field of plant molecular biology, and relates to application of VDAC1 / 3a allele in regulation of tomato cold resistance. According to the invention, the expression quantity of the SlVDAC1 / 3a gene in the VDAC1 / 3a allele is reduced by means of knockout or mutation and the like, so that the cold resistance of the tomato can be enhanced, the yield of the tomato and the content of soluble solids are not negatively influenced, and a theoretical basis and gene resources are provided for cultivating and screening cold-resistant tomato varieties.

The invention discloses preparation and application of an arundina graminifolia fermentation extract. The arundina graminifolia is fermented by adopting saccharomyces cerevisiae, so that the yield of total polyphenol (increased by 119.1%) and the yield of flavone (increased by 43.8%) in an arundina graminifolia extracting solution can be remarkably increased. The content of soluble solids in the obtained fermentation broth is 9.12 mg / mL, and the fermentation broth has acetylcholin esterase inhibitory activity (IC50 is 4.0 mg / mL) and oxidation resistance (the free radical scavenging rates IC50 of ABTS and DPPH are 0.116 mg / mL and 16.2 [mu] g / mL respectively); the fermentation liquor is high in sensory evaluation and palatability, and can be applied to nerve health maintenance in the field of homology of medicine and food as well as antioxidant functional beverages and health care products.

The invention discloses an application of a tomato B3-337 transcription factor in improvement of tomato quality, a gene for coding a B3-337 transcription factor protein is named as a B3-337 gene, and the nucleotide sequence is shown as SEQ ID NO.1. The invention also discloses an application of the tomato B3-337 transcription factor in improvement of tomato quality. According to the application, a carrier pFM3100GFP-B3-337 is constructed, so that the B3-337 is proved to be positioned in a cell nucleus. A transcriptional activity detection result proves that B3-337 has transcriptional activation activity. RT-qPCR (real-time quantitative polymerase chain reaction) and GUS (glucuronidase) dyeing show that the transcription factor is expressed in pericarp, seed, placenta and center pillar of each fruit ripening stage. According to the invention, a B3-337 gene overexpression plant is obtained by constructing a B3-337 overexpression vector, and compared with a wild type, the tomato fruit overexpressed with the B3-337 gene has the advantages that the content of soluble solids brix is increased, the fruit becomes larger, and the weight becomes heavier. It is shown that the B3-337 transcription factor plays an important regulation role in positive regulation of tomato fruit quality. The invention lays a good gene genetic resource foundation for the cultivation of new varieties of high-quality tomatoes.

The invention discloses an SNP molecular marker for identifying the soluble solid content of peach fruits and application thereof, the nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.1, and the SNP mutation site is the 201st site (A / G mutation) of the sequence as shown in SEQ ID NO.1 and is located at 16bp, 613bp and 334bp of a fifth chromosome of a peach genome. The SSC contents are classified into two classes by taking 12% as a boundary in combination with genotypes by referring to Peach germplasm Resource Description Standards and Data Standards, that is, the SSC content smaller than or equal to 12% is low SSC content (AA / AG), and the SSC content larger than 12% is high SSC content (GG). The obtained SNP molecular marker is used for verifying the content of soluble solids in pulp, and the result shows that the accuracy rate of the low SSC content (AA / AG) is 84.48%, the accuracy rate of the high SSC content (GG) is 81.69%, and the overall accuracy rate is 83.23%, which shows that the SNP molecular marker can be used for early prediction and screening of the content of soluble solids in peach fruits.

The invention provides a method for improving storage and preservation of fresh lilies, which comprises the following steps of: soaking the fresh lilies in micro-nano bubble ozone water (O3-MNBW), naturally airing after soaking treatment, and storing under a cold storage condition. The invention also provides a new application of the micro-nano bubble ozone water, namely the application of the micro-nano bubble ozone water in improving the storage quality of the picked fresh lily. According to the method provided by the invention, the weightlessness of the lilium brownii scales is obviously slowed down, the weightlessness, hardness reduction, respiration and browning of the lilium brownii scales are effectively inhibited, the content of soluble solids is maintained, the dip dyeing of pathogenic microorganisms is effectively prevented, and the quality of the lilium brownii scales in the postharvest storage period is obviously improved. Through O < 3 >-MNBW treatment, the sensory evaluation index of the picked fresh lilies is effectively improved, the preservation quality is improved, browning after picking is inhibited, only one-time treatment is needed before storage, and the economic benefit is remarkable. And a novel, economical and effective fresh-keeping mode is provided for the storage period of the picked fruits and vegetables.

The invention belongs to the technical field of microbial fertilizers, and particularly relates to a disease and pest resistant microbial fertilizer based on traditional Chinese medicinal materials and a preparation method. The pest and disease resistant microbial fertilizer is prepared from the following raw materials in parts by mass: 15-25 parts of a traditional Chinese medicinal material extract, 70-100 parts of a composite carrier, 2-4 parts of composite microbial flora, 5-10 parts of potassium fulvate, 5-10 parts of sodium humate, 5-10 parts of polyglutamic acid and 15-25 parts of water, the pest and disease resistant microbial fertilizer provided by the invention not only has high viable count, but also has good stability, and can inhibit powdery mildew, downy mildew and aphids of cucumbers at the same time, so that the cucumbers have high acre yield, soluble total sugar content and soluble solid content.

The invention relates to a method for improving portable near infrared spectrum data analysis precision, which comprises the following steps: carrying out spectrum scanning on different grape samples to obtain a plurality of original near infrared spectrum data; preprocessing the original near infrared spectrum data to obtain corresponding preprocessed data, and performing dimension transformation on the preprocessed data to obtain a two-dimensional image; processing the two-dimensional image to obtain a reconstructed image, and training the initial convolutional neural network model based on the reconstructed image to obtain a target convolutional neural network model; and taking the output result of the feature extraction layer of the target convolutional neural network model as the input of a partial least square regression model, and constructing a fusion model so as to detect the soluble solid content of the to-be-detected grape through the fusion model. According to the invention, the hardware limitation of the portable near-infrared spectrometer is overcome, and the precision and efficiency of the portable near-infrared spectrum data analysis precision are improved.

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

The invention belongs to the technical field of fruit tree cultivation and plant growth regulation, and particularly relates to a plant growth regulator containing 5-ALA and application of the plant growth regulator in prunus humilis planting. According to the method disclosed by the invention, the nutritional quality of fruits is remarkably improved by spraying a 100 mg / L 5-ALA solution on the whole cerasus humilis plant. Compared with clear water, the sugar content of the cerasus humilis fruits treated by the 5-ALA in the full ripening stage is increased by 44.01%, the acidity is reduced by 23.75%, the soluble solids are increased by 60.22%, and the flavor of the fruits is remarkably improved; meanwhile, the total calcium content of the fruits is increased by 30.22%, the water-soluble calcium content is increased by 91.12%, the pectin calcium content is reduced by 30.04%, and the active calcium content is increased by 50.74%; the content of soluble protein is increased by 30.98%, so that the acidity is reduced, and sugar, calcium and protein are synergistically improved.

The invention discloses an American ginseng fermentation extract and application thereof, and belongs to the technical field of traditional Chinese medicine fermentation and cosmetics. According to the method, American ginseng is fermented by adopting bacillus mojavensis, and operation and parameters of links such as preparation, fermentation, purification and freeze drying of a fermentation substrate are regulated and controlled, so that various enzymes generated in the metabolic process of the bacillus mojavensis are used for converting and modifying various active ingredients in the American ginseng, and the biological activity of the American ginseng is enhanced. Compared with unfermented American ginseng, the American ginseng fermentation extract prepared by the preparation method disclosed by the invention has the advantages that the soluble solids are obviously increased, the contents of active substances such as saponin and polyphenol are also obviously increased, the antioxidant activity is also obviously improved, and the American ginseng fermentation extract has an obvious improvement effect on oxidative damage cells, effectively improves the skin microenvironment and achieves the purpose of skin care; the important significance is realized on the development of anti-aging cosmetics.

The invention discloses application of an SlBRL2 gene in improvement of tomato fruit quality, and belongs to the technical field of plant genetic engineering. According to the invention, the gene SlBRL2 related to the tomato fruit quality is found, and the CDS sequence of the gene SlBRL2 is as shown in SEQ ID NO. 1. It is found for the first time that the knockout of the SlBRL2 gene can significantly improve the tomato fruit shape index, the fruit hardness, the soluble solid content, the total sugar content and the carotenoid content, the taste and the nutritional quality of tomatoes are improved while the storage resistance and the transportation resistance of the tomatoes are improved, and a new direction is provided for tomato germplasm breeding.

According to the method for prolonging the fresh-keeping period of the fruits by using the controlled atmosphere fresh-keeping box, the controlled atmosphere fresh-keeping box is prepared by improving the sealing performance of a common fresh-keeping box and covering a certain area of the common fresh-keeping box with a film, and the fruits are put into the box to be stored. When the area of the covering film is 20%, the lotus seeds are stored in a box, and 1-methylcyclopropene can be added; when the area of the covering film is 34.8%, the longan is placed in a box to be stored. Compared with the control, the modified atmosphere preservation box treatment can reduce the weight loss rate and browning degree of the lotus seeds and maintain the contents of Vc, amylose, total phenols and soluble solids; compared with a control group, the modified atmosphere preservation box treatment can inhibit browning of longan peel, delay reduction of soluble solids, acidity and vitamin C, inhibit increase of malondialdehyde content, reduce peroxidase activity and improve catalase activity and glutathione content. According to the technology, the shelf life of the fruits can be effectively prolonged, and the problems that an existing fresh-keeping method is tedious and short in fresh-keeping period are solved.

The invention relates to the technical field of agricultural product detection, and discloses a grape SSC detection and origin traceability method based on NIR multi-task ensemble learning. The method comprises the following steps: (1) carrying out near infrared spectrum transmission scanning on a grape sample, and measuring the content of soluble solids; (2) integrating the spectral data, the soluble solid content and different production place categories, and splitting into a training set and a test set; (3) identifying soluble solid content characteristics and production place category characteristics by using a Boruta algorithm, and calculating average importance after the characteristics are combined; (4) setting an importance threshold, screening out shared features, and obtaining a reconstruction training set and a reconstruction test set; (5) constructing an XGBoost integrated learning model by using the reconstructed training set, carrying out global tuning on hyper-parameters, and training to obtain a final model; and (6) performing prediction based on the final model to complete grape SSC detection and origin traceability. The method provided by the invention can overcome the defects of low feature utilization rate and limited precision of a traditional single-task model.

The invention discloses an application of a Solyc02g060560 gene in regulation and control of sugar content and size of tomato fruits, wherein a CDS (Coding Sequence) sequence of the Solyc02g060560 gene is as shown in SEQ ID NO. 1. A tomato transcription factor mutant library is constructed through a CRISPR (clustered regularly interspaced short palindromic repeats) technology, and a mutant TOM6-126 with unknown functions at present in a C2H2 family is screened. Based on observation of the phenotype of the mutant, the inventor determines that the gene is related to the sugar content and the fruit size of tomato fruits. Compared with a wild type, the soluble solid content (brix) of tomato fruits of the mutant is increased, and the fruits become larger. It is indicated that the Solyc02g060560 gene plays an important role in negative regulation of tomato fruit growth and development and sugar content. According to the invention, a good gene genetic resource foundation is laid for the cultivation of a new variety of tomatoes with high sugar content.

The invention discloses a method for rapidly detecting the quality of potatoes in low-temperature storage and normal-temperature sugar recovery processes, and belongs to the technical field of agricultural product detection. Obtaining multi-variety colored potato samples under different storage conditions and storage time; physicochemical determination is carried out on four quality indexes of potato starch, soluble solids, moisture and hardness in the storage process; acquiring an absorption coefficient spectrum and a reduced scattering coefficient spectrum of the sample slice in a range of 400-1700nm; based on the absorption coefficient spectrum and the reduced scattering coefficient spectrum, establishing and selecting an optimal quantitative prediction model of each quality index of the potatoes through different spectrum preprocessing, spectrum dimension reduction and feature extraction algorithms and a machine learning regression algorithm; and using the optimal model to rapidly detect the corresponding quality index. The method can provide a new rapid and integrated detection means for the key physical and chemical quality of the potatoes in the low-temperature storage and normal-temperature sugar return treatment processes.

The invention discloses a plant composition with a fat reducing effect and a preparation method of a fermentation extract of the plant composition. Mangnolia officinalis, pepper, astragalus membranaceus, honeysuckle, salvia miltiorrhiza and immature bitter orange are combined together according to the compatibility of monarch, minister, assistant and guide to obtain an extract fermented by yeast, polyphenol components of the extract are enriched after impurity removal and purification, total polyphenol and soluble solids are taken as index contents, the extract can be prepared into an external fat reducing agent for use, qi and blood circulation of a body is facilitated, fat metabolism is regulated, and the fat reducing effect is improved. And the tea is safe and mild, and has the effects of reducing fat, losing weight, slimming, shaping and delaying senescence.

The invention belongs to the technical field of genetic engineering breeding, and particularly relates to application of an SC8 gene in regulating accumulation of soluble solids in tomato fruits and a breeding method. The SC8 gene provided by the invention plays an important role in tomato saccharic acid metabolism and soluble solid synthesis and accumulation, and plays a role in negative regulation of tomato fruit soluble solid synthesis and accumulation. The SC8 gene is applied to plant genetic engineering breeding, and a new functional site and a new breeding thought are provided for cultivating high-quality flavor tomato varieties. By inhibiting the expression of the SC8 gene, soluble solids and sugar acid metabolites thereof such as cane sugar, fructose, glucose, malic acid and citric acid in the red ripe tomato fruits can be increased, and by enhancing the expression level of the SC8 gene, synthesis and accumulation of the soluble solids in the tomato can be reduced; therefore, the implementation of the invention provides a feasible way for cultivating and producing tomatoes with various flavors and qualities, and plays an important role in improving the economic benefits of the tomato industry.

The invention relates to the technical field of gene engineering, in particular to application of a knockout SlABCB13 gene in increasing the soluble sugar content of tomato fruits. The function of the SlABCB13 gene is deleted by mediating gene editing, so that the content of soluble sugar (cane sugar, fructose and glucose) and the content of soluble solids in tomato fruits are remarkably increased, and the SlABCB13 participates in sugar transport of the tomato fruits. Meanwhile, the mutant does not influence related yield indexes such as plant height, stem diameter, fruit size, fruit setting number and single-plant fruit yield, and a new thought is provided for the problem that yield and quality are difficult to consider in existing tomato breeding.

Provided herein are plants and plant parts (e.g., tomato plants and plant parts) comprising altered expression or function of the bZIP transcription factor, plants and plant parts comprising one or more mutations in the upstream regulatory region of the bZIP transcription factor gene (e.g., bZIP SIRT element), and plants and plant parts having increased sugar and / or total soluble solids levels. Also disclosed herein are methods of producing such plants and plant parts.

To provide a food which does not require refrigeration for preservation and enables preparation of a pasta dish excellent in quality with reduced work.SOLUTION: A food with instruction for microwave oven cooking is used for heat-cooking dried pasta in diluted pasta sauce by microwave heating. The food includes: pasta sauce which contains a seasoning liquid and solid ingredients and produces diluted pasta sauce containing a water diluted liquid of the seasoning liquid and the solid ingredients by dilution with water; and an instruction describing an amount of water for diluting pasta sauce and a heating condition and a steaming condition of the diluted pasta sauce containing the dried pasta. In the diluted pasta sauce produced when the pasta sauce is diluted by the amount of water, which is described in the instruction, viscosity of the water diluted liquid at 25°C is 130 mPa s or less and soluble solid concentration of the water diluted liquid at 25°C is 16% by mass or less.SELECTED DRAWING: None

The preparation method comprises the following steps: (1) raw material pretreatment: respectively weighing millettia specisoa, angelica sinensis and astragalus membranaceus, adding a proper amount of warm water, and soaking to obtain pretreated raw materials; (2) pretreatment of pigtails: cutting off and crushing the pigtails, washing the pigtails, blanching the pigtails to remove fishy smell, filtering and washing the pigtails, and putting the pigtails under a cold storage condition for later use; and (3) cooking treatment: adding the pretreated raw materials in the step (1) and a proper amount of water and salt into the pretreated pigtails, then putting into an aluminum foil vacuum bag, sealing, and decocting in a high-pressure steam sterilization pot to obtain the beautiful millettia root pigtail soup. The prepared beautiful millettia root and pigtail soup is high in sensory score, soluble protein content and soluble solid content, and the obtained soup-stock is high in total antioxidant capacity and hydroxyl free radical scavenging rate.

The application discloses a concentrated tomato product for assisting in reducing blood fat and a preparation method thereof. The concentrated tomato product is prepared from the following raw materials in parts by weight: mature red tomatoes 800-1000 parts, phytosterols 8-12 parts, composite dietary fibers 3-8 parts, Omega-3 fatty acid microcapsules 2-6 parts, antioxidants 0.5-1.2 parts, stabilizers 0.3-0.8 parts, and edible buffer agents in an appropriate amount. The concentrated tomato product is concentrated juice or powder. The soluble solid content of the concentrated juice is 35-40 Bx, and the moisture content of the powder product is 5.0%. The application combines phytosterols, composite dietary fibers, Omega-3 fatty acid microcapsules and other natural functional components, and the components play a synergistic effect through different blood fat reduction mechanisms, thereby comprehensively assisting in regulating lipid indexes such as cholesterol and triglyceride. The blood fat reduction effect is significantly better than that of single-component products and ordinary tomato products, and the health needs of people with high blood fat can be met.

The present application belongs to the technical field of plant cultivation, and particularly relates to a biological fertilizer for relieving fruit drop of Taiwan long fruit mulberry and application. The biological fertilizer comprises animal manure, soybean meal powder, humic acid, peat soil, coconut bran, bran, fermentation liquor of a compound microbial agent, seaweed extract powder, amino acid chelated iron and borax; wherein the compound microbial agent comprises Bacillus amyloliquefaciens and Pseudomonas aeruginosa. The above biological fertilizer is balanced in nutrients, is beneficial to enhancing the resistance of the mulberry to adversity and reducing fruit drop caused by stress; in addition, the Bacillus amyloliquefaciens and the Pseudomonas aeruginosa are used synergistically, and the two jointly build a healthy rhizosphere micro-ecology, thereby guaranteeing nutrient supply for fruit development from the root source. Therefore, the technical scheme of the present application can not only significantly reduce the fruit drop rate, but also improve the single fruit weight, soluble solid content and anthocyanin content of mulberry, and improve the flavor and color of the fruit.

An aloe extract-based antibacterial composition, which is a nano microemulsion and comprises the following components in percentage by mass according to soluble solids: 0.50%-10% of an aloe extract, 0.50%-5.0% of polylysine, 0.50%-20% of a caprylohydroxamic acid, 6.0%-20% of a surfactant, 15%-60% of a solvent, the remainder being water. A preparation method for the antibacterial composition comprises: mixing polylysine, an aloe extract, a caprylohydroxamic acid, a surfactant, a solvent, an optional auxiliary agent, and water, and carrying out ultrasonic treatment to prepare a uniform nano microemulsion. The aloe extract, the polylysine and the caprylohydroxamic acid are compounded by means of a microemulsion system, thereby achieving a synergistic effect, and greatly improving an antibacterial effect; and the antibacterial composition is green and safe and can serve as a bacteriostatic agent to be used in products such as daily necessities, washing and caring cosmetics and baby products.

The invention discloses an application of indole-3-acetamide in storage and preservation of postharvest oranges, and belongs to the technical field of food preservation. A variety of indole-3-acetic acid analogues are selected, a postharvest storage and fresh-keeping test is carried out by taking citrus reiculata Blanco as an experimental material, and the screened indole-3-acetamide has stronger fresh-keeping activity and can greatly prolong the storage period of citrus reiculata Blanco. Then, Wenzhou mandarin oranges are used as experimental materials to further verify the effect of the indole-3-acetamide in citrus preservation, and it is proved that the indole-3-acetamide can significantly reduce the pedicel aging rate and rotting rate; the accumulation of peel reactive oxygen species (ROS) is inhibited, and the SOD activity is improved; and the fruit hardness, soluble solids and sugar-acid ratio are maintained, and the internal quality is maintained. Therefore, as an indole-3-acetic acid analogue, the indole-3-acetamide has excellent storage and fresh-keeping effects after the citrus is picked.

The invention belongs to the technical field of molecular breeding, and discloses application of a citrus CsAPD2-2 gene in reducing the citric acid content of citrus fruits. The invention discloses the application of the CsAPD2-2 gene in regulating and controlling the citric acid content of plant leaves / fruits for the first time. According to the invention, the sugar orange CsAPD2-2 gene highly associated with the citric acid content of the fruits is successfully cloned, and the gene is specifically and highly expressed in the citrus fruits. The gene is over-expressed in citrus and tomato, or the gene is knocked out in citrus by virtue of a CRISPR / Cas9 gene editing technology, so that the content of citric acid in fruits is correspondingly reduced and increased, and meanwhile, the gene does not influence the properties of flowering and fruiting time, pulp color, single fruit weight, soluble solid content and the like of plants; therefore, the CsAPD2-2 gene is a fruit citric acid content negative regulation gene with strong pertinence.