82results about How to "Detection content" patented technology

The invention provides a quick detection method for detecting the content of various compositions in honey by utilization of a mathematical model between a near infrared spectrum of the honey and the contents of the various compositions of the honey, in order to overcome the defects of complex operation and labor and time consumption in a chemical and chromatographic analysis method for detecting the quality of the honey. The quick detection method for the quality of the honey is as follows: firstly, obtaining a honey sample in the homogeneous fluid state; secondly, acquiring the near infrared spectrum; and thirdly, converting the near infrared spectrum acquired into the quality parameter of the honey by utilization of the mathematical model. The quick detection method for the quality of the honey can quickly and accurately detect the contents of water, soluble solid content, organic acid, fructose, glucose, sucrose and maltose in the honey.

The invention discloses a method for quickly detecting protein and amino acid in rapeseeds. The method comprises the following steps of: A, drying harvested rapeseeds in a drying oven, controlling the humidity for drying at 103 and 108 DEG C, controlling the time between 3 and 5 hours, and then cooling the rapeseeds to the room temperature; B, putting the cooled rapeseeds into a sample tray of a near infrared instrument to scan a near infrared spectrum, and repeating the sample loading for 2 to 4 times each time; C, averaging the spectrums measured by 2 to 4 times of a sample to calculate the average spectrum; and D, substituting the average spectrum into a model to calculating the content of crude protein and the amino acid of the sample. The method is easy to implement, is simple and convenient to operate, and has simple pretreatment of the sample. For a rapeseed manufacturer, the harvested rapeseeds are quickly measured at a certain temperature during a certain time. The method is quick and causes no damages. The acquisition time of the near infrared spectrum is very short, and the model calculation time can be basically ignored. By using the method, a plurality of components can be measured at the same time, and the content of the crude protein and the content of other amino acid in the rapeseeds can be measured at the same time.

The invention discloses a fluorescent probe for detecting microcystic toxin-LR in water, which belongs to the field of molecular biology and microbiology. According to the fluorescent probe, MC-LR is taken as a target, a dodecapeptide library randomly shown on the surface of a bacteriophage is used for selecting a recombination bacteriophage which is combined with the MC-LR in a specific manner, and the single-stranded DNA (Deoxyribonucleic Acid) of the recombination bacteriophage is extracted and recombined for sequencing and sequence alignment, thereby obtaining an MC-LR specific affinity ligand sequence: His-Phe-Phe-Lys-Trp-His-Thr-Arg-Thr-Asn-Asp-Gln, and subsequently, obtaining the specifically combined fluorescent probe FITC-Acp-His-Phe-Phe-Lys-Trp-His-Thr-Arg-Thr-Asn-Asp-Gln through solid-phase polypeptide synthesis and fluorescent marks. The fluorescent probe has the beneficial effects that firstly, a completely new tool is provided for the specific identification and content detection of the MC-LR; and secondly, the fluorescent ligand probe can be used for qualitatively identifying the MC-LR and quantitatively detecting the content of the MC-LR in a sample.

The invention discloses a quick detector for quality and safety of tea. The detector is provided with a machine shell of which the upper surface is provided with a color comparison cuvette cover. Thedetector is characterized in that: a transmission type color comparison cuvette and a reflection type color comparison cuvette are arranged side by side under the color comparison cuvette cover of themachine shell; the transmission type color comparison cuvette is a rectangular tank body, one side surface of the tank body is provided with a light source/monochromator, and the other opposite sidesurface is provided with a photoelectric detector; and the reflection type color comparison cuvette is a cuboid of which the inside is provided with three T-shaped channel holes, channel openings of two side channel holes are provided with a light source/monochromator respectively, the lower part channel opening of the middle channel hole is provided with a photoelectric detector, and the lower part channel opening of the middle channel hole is provided with a chromatographic plate. The detector can be used for quickly and quantitatively detecting the contents of heavy metal and pesticide in tea in field and quickly detect the quality safety of tea in field.

The invention discloses a fluorescent probe for detection of cystatin C and a construction method thereof, relates to the molecular biological and microbiological technical fields, and is characterized in that the construction method comprises the steps: with Cys-C as a target, screening to obtain a Cys-C specific-binding recombinant phage by using a phage surface random displayed 12-mer peptide library, extracting single stranded DNA of the recombinant phage, carrying out sequencing and sequence comparison, to obtain a sequence Gln-Val-Asn-Gly-Leu-Gly-Glu-Arg-Ser-Gln-Gln-Met of a Cys-C specific affinity ligand having the molecular weight of 1346.5, then carrying out solid phase polypeptide synthesis and fluorescence labeling to obtain the fluorescent probe FITC-Acp-Gln-Val-Asn-Gly-Leu-Gly-Glu-Arg-Ser-Gln-Gln-Met having the molecular weight of 1848.7 and specifically bond with the Cys-C. The fluorescent probe and the construction method thereof have the beneficial effects: 1, a brand new 'tool' is provided for specific identification and content detection of the Cys-C; and 2, the fluorescent ligand peptide probe not only can qualitatively identify the Cys-C, but also can quantitatively detect the content of the Cys-C in samples.

The invention discloses a composition and a kit for detecting the content of donkey hide source component in tortoise shell gelatin, deer horn gelatin and fake gelatin, and a detection method thereof. The composition comprises polypeptide I, polypeptide II, polypeptide III and control detection matrix, wherein the amino acid sequence of the polypeptide I is shown as SEQ ID NO.1, the amino acid sequence of the polypeptide II is shown as SEQ ID NO.2, the amino acid sequence of the polypeptide III is shown as SEQ ID NO.3, and the control detection matrix is fishskin gelatin. The detection value of the polypeptide II is subtracted from the detection value of the polypeptide I, and the detection deviation can be corrected by utilizing the detection value of the polypeptide III, so that the detection of content of donkey hide source component in the tortoise shell gelatin, deer horn gelatin and fake gelatin can be realized. The method has the advantages of being accurate in content detection, and simple to operate, and has wide application prospects on the true and false identification aspect of tortoise shell gelatin, deer horn gelatin and fake gelatin.

The invention provides a method for detecting glyceryl monostearate in liquid milk. The method comprises the following steps: a. preparing a bottle of liquid milk to be tested, extracting milk fat from the liquid milk so as to obtain milk fat to be tested; b. preparing a standard solution of glyceryl monostearate, blow-drying the standard solution with nitrogen gas so as to obtain a reference substance; c. separately adding N,O-bis(trimethylsilyl)trifluoroacetamide into the milk fat to be tested and the reference substance to carry out derivatization reactions so as to separately obtain a derivative sample and a derivative reference substance; d. blow-drying the derivative sample and the derivative reference substance with nitrogen gas, then separately dissolving the derivative sample and the derivative reference substance with n-hexane so as to separately obtain a sample liquid to be tested and a reference detection liquid; e. measuring the gas chromatography values of glyceryl monostearate in the sample liquid to be tested and the reference detection liquid, and calculating according to the gas chromatography values of glyceryl monostearate in the sample liquid to be tested and the reference detection liquid so as to obtain the content of glyceryl monostearate in the liquid milk to be tested. The detection method has the advantages of convenient operation and high accuracy.

The invention provides a method for determining an aminoglycoside drug in an animal muscle tissue without using of an ion pair reagent, and belongs to the technical field of veterinary drug residue detection, and the method comprises performing liquid chromatography-tandem mass spectrometry detection on an injection sample, and obtaining the content of the aminoglycoside drug in the injection sample according to a standard curve of the content of the aminoglycoside drug and a liquid chromatogram of the injection sample; wherein a liquid chromatography column is SIELC Obelisc R. The method provided by the invention can enhance the chromatographic retention without using of the ion pair reagent, and can detect the content of the aminoglycoside drug the animal muscle tissue, the detection limit and the limit quantification of the drug are respectively in a range of 1 to 10 mu g/kg and a range of 2-20 mu g/kg; within-run precision and between-run precision are 2-14.4% and 3.7-19.8% respectively.

The invention provides a preparation method of high-purity 3-deacetylcephalosporin C sodium salt. The preparation method comprises the following steps: (1) dissolving; (2) cracking; (3) crystallizing;and (4) filtering and drying. According to the preparation method, local peracid in the crystallization process can be avoided, degradation is reduced, the usage amount of concentrated hydrochloric acid and ammonia water is reduced, obvious economic benefits are achieved, meanwhile, erosion of chloride ions to production equipment is reduced, loss of the equipment is reduced, the volume of a mother liquor can be reduced, and the yield is increased. The purity of the 3-deacetylcephalosporin C sodium salt prepared by the preparation method disclosed by the invention is greater than 99% of DCPC;when the3-deacetylcephalosporin C sodium salt is used as a reference substance, the content of DCPC can be more accurately detected, the content of DCPC can be better controlled and reduced in CPC, DAOC and 7-ACA and 7-ADCA production, and the product quality is improved.

The invention discloses a method for detecting dehydrogenized vitamin C in vitamin C and belongs to the field of analytic chemistry. The method is characterized in that the method uses liquid chromatography and an HILIC affinity chromatography column and uses acetonitrile--isopropanol-water as the mobile phase A and acetonitrile-water as the mobile phase B to perform analysis through gradient elution. The method has the advantages that by the method, the content of the dehydrogenized vitamin C can be analyzed fast and accurately; the quantification limit of the method can reach 0.38 microgram/ml, the detection limit of the method is 0.11 microgram/ml, the average yield of the method is 100.5%, and the concentration of the dehydrogenized vitamin C and peak area present a good linear relation in the range from 0.38 microgram/ml to 77.75 microgram/ml; the liquid-phase method is also applicable to vitamin quality researches such as researches on the stability and degradation of vitamin C medicine, compound vitamin (3) for injection and compound vitamin (13) injection.

The invention provides a method for detecting purithiamine in blood by efficient liquid chromatography. The method is characterized in that the method comprises the specific steps: taking whole blood, removing protein, and using the efficient liquid chromatography to detect, wherein the condition of the chromatography comprises: using C18 chromatographic column; taking disodium hydrogen phosphate solution and monosodium orthophosphate obtained in step 1 as a first mobile phase and a second mobile phase respectively; and gradually eluting at the flow speed of 1.0 mL/min, wherein the exciting wavelength of fluoroscopic detection is 385nm and the emitting wavelength is 460nm. The invention has the advantage that the content of the purithiamine in the blood can be detected by a simple method.

The invention discloses a hydrogen cyanide detection reagent and a preparation method thereof and belongs to the technical field of gas detection. The reagent includes the components including, by weights: 3 to 6 parts of sodium hydroxide, 5 to 10 parts of phenol resin, 3 to 6 parts of aceton, 9 to 19 parts of helianthin B, 3 to 7 parts of chrysolepic acid, 2 to 5 parts of polyacrylate, 6 to 11 parts of ethanol and 10 to 15 parts of distilled water. The method includes the steps of 1, preparing a premix liquid; 2, obtaining a clear premix liquid; 3, standing for 12 to 48 hours in vacuum, sealing, and obtaining the hydrogen cyanide detection reagent. The obtained hydrogen cyanide detection reagent has the advantages of high reaction flexibility and accuracy, the content of hydrogen cyanide can be detected effectively, and the damage can be reduced.

The invention provides a method for detecting 2, 4-dichlorphenoxyacetic acid and belongs to the technical field of pesticide detection. The method comprises steps of an alkaline phosphatase solution and a sample being mixed and left to stand, and standing liquid being obtained; mixing the standing liquid with a sodium pyrophosphate solution and a Tris-HCl buffer solution, and carrying out a reaction to obtain a reaction liquid; mixing the reaction solution with a cerous nitrate solution, oscillating to obtain an oscillating solution, measuring the fluorescence emission spectrum of the oscillating solution to obtain the maximum fluorescence intensity, and converting the maximum fluorescence intensity into an enzyme inhibition rate; and substituting the enzyme inhibition ratio into the linear equation to obtain the concentration of 2, 4-dichlorophenoxyacetic acid in the sample. By adopting the method provided by the invention, a detection probe does not need to be prepared, and a detection result can be obtained within 45 minutes; the linear range of the linear equation is 2.5-200 [mu] g/L, and the detection limit is 0.98 [mu] g/L.

The invention belongs to the technical field of biology, and provides an anti-SpA5 protein monoclonal antibody, application thereof, and a kit containing the monoclonal antibody. The anti-SpA5 proteinmonoclonal antibody comprises a heavy chain and a light chain, wherein the heavy chain and the light chain respectively comprise three CDR regions, the amino acid sequences of the heavy chain CDR regions are respectively as shown in SEQ ID NO: 1-3, and the amino acid sequences of the light chain CDR regions are respectively as shown in SEQ ID NO: 4-6. The antibody disclosed by the invention can be specifically combined with SpA5 protein and effectively detect the content of the SpA5 protein, and has high sensitivity and wide detection range.

The invention relates to a transmission-type optical detection device for an intelligent expiration molecular diagnosis system. The detection device comprises a reaction tank, a biologic sensor and a light path structure for spectral analysis, wherein the biologic sensor comprises a reactant which can react with and absorb a biomarker exhaled by a human body; a reaction chamber is arranged in the reaction tank; the reaction chamber is communicated with an external human exhaled air source; the biologic sensor is arranged in the reaction chamber and the reactant is packaged in the reaction chamber; the light path structure can generate light beam and inject into the reaction chamber; after the light beam passes by the reactant, the light beam is emitted out from the reaction tank, so as to form an absorption spectrum of the reactant. The transmission-type optical detection device for the intelligent expiration molecular diagnosis system adopts the reactant of the biologic sensor for reacting with the biomarker exhaled by the human body, the light path structure for performing spectral analysis on the reactant is adopted for detecting the reactant after absorbing the biomarker, the content of the biomarker exhaled by the human body can be quickly and accurately detected and the precision is high.

The invention provides environment-friendly intelligent septic tank purification equipment and a purification method thereof. The equipment comprises a vehicle body, a vacuum tank, a vacuumizing device, a sewage inlet pipe, a sewage pump, a filter press, a clear water tank, a sewage pumping pipe, a rotary solid-liquid separator, a garbage chamber, an agent feeding tank, a detection device, a purification device and a two-way water pump. The vacuum tank is mounted on the upper surface of the vehicle body through bolts, and the vacuumizing device is mounted at the right end of the vacuum tank atthe bottom of the vehicle body through bolts. The rotary solid-liquid separator is mounted on the inner right side of the vacuum tank through bolts, separation of large solid impurities and liquid can be realized beneficially, the large solid impurities are sent into the garbage chamber, and liquid substances enter a press filter through the sewage inlet pipe to realize solid-liquid separation. The two-way water pump is mounted at the outer upper end of the vacuum tank through bolts and connected with the clear water tank and the purification device, sewage treatment conditions in a detectiontank can be well detected, the two-way water pump is available for recovery treatment, and high efficiency, quickness and intelligence in a whole treatment process are realized.

The invention provides a method for detecting active metal elements in hard zinc. The method comprises the following steps that 1, roasted or oxidized extracted hard zinc slag is subjected to ball milling and sifting through a 50-150 mesh sieve; 2, the sifted hard zinc slag is added to a copper sulfate solution for heating, reaction and filtering to obtain a filtrate and filter slag, wherein the content of copper in the copper sulfate solution is 50-10000 ppm, and the mass ratio of the copper sulfate solution to the sifted hard zinc slag is (3-20): 1; 3, the content of copper in the filtrate is detected to obtain the content of active metals in the hard zinc slag. The method utilizes the reaction of the active metals with copper sulfate to cause the change of the copper content, calculatesthe content of the active metals in the roasted or oxidized hard zinc slag, and can quantitatively detect the content of the active metal elements in the slag; the method can not only quantify, but also can detect slag with the active metal content of 10 ppm, and solves the difficult problem of quantitative detection of trace active metal elements in solid slag.