82results about How to "Detection content" patented technology

The invention provides a quick detection method for detecting the content of various compositions in honey by utilization of a mathematical model between a near infrared spectrum of the honey and the contents of the various compositions of the honey, in order to overcome the defects of complex operation and labor and time consumption in a chemical and chromatographic analysis method for detecting the quality of the honey. The quick detection method for the quality of the honey is as follows: firstly, obtaining a honey sample in the homogeneous fluid state; secondly, acquiring the near infrared spectrum; and thirdly, converting the near infrared spectrum acquired into the quality parameter of the honey by utilization of the mathematical model. The quick detection method for the quality of the honey can quickly and accurately detect the contents of water, soluble solid content, organic acid, fructose, glucose, sucrose and maltose in the honey.

The invention provides a method for simultaneously detecting multiple lipid-soluble vitamin contents in blood. The method comprises the following steps: detecting at least three standard solutions byutilizing a high-performance liquid chromatograph tandem mass spectrometer so as to obtain a corresponding chromatogram, wherein each standard solution contains multiple vitamins and internal standardsubstances thereof with known concentrations; fitting to obtain standard curve equations of various vitamins according to the concentration specific value of various vitamins and the internal standard substances thereof in the standard solutions, and the peak area specific value of the vitamin and the internal standard substance in the corresponding chromatogram; treating to-be-detected blood toobtain a blood sample, and adding multiple internal standard substances with known concentrations, and performing sample pre-treatment to obtain a to-be-detected sample; detecting the to-be-detected sample under the same detection condition to obtain the chromatogram thereof; substituting the peak area specific value of each vitamin and the internal standard substance thereof in the chromatogram into the corresponding standard curve equation, and computing the content of each vitamin in the to-be-detected blood according to the concentration of the added internal standard substances thereof. Through the method provided by the invention, the contents of various lipid-soluble vitamin contents in the blood can be detected at the same time.

The invention discloses a method for quickly detecting protein and amino acid in rapeseeds. The method comprises the following steps of: A, drying harvested rapeseeds in a drying oven, controlling the humidity for drying at 103 and 108 DEG C, controlling the time between 3 and 5 hours, and then cooling the rapeseeds to the room temperature; B, putting the cooled rapeseeds into a sample tray of a near infrared instrument to scan a near infrared spectrum, and repeating the sample loading for 2 to 4 times each time; C, averaging the spectrums measured by 2 to 4 times of a sample to calculate the average spectrum; and D, substituting the average spectrum into a model to calculating the content of crude protein and the amino acid of the sample. The method is easy to implement, is simple and convenient to operate, and has simple pretreatment of the sample. For a rapeseed manufacturer, the harvested rapeseeds are quickly measured at a certain temperature during a certain time. The method is quick and causes no damages. The acquisition time of the near infrared spectrum is very short, and the model calculation time can be basically ignored. By using the method, a plurality of components can be measured at the same time, and the content of the crude protein and the content of other amino acid in the rapeseeds can be measured at the same time.

The invention provides a method for simply and quickly detecting hoptoglobin in serum, namely an immunoturbidimetry, and a detecting kit thereof. The method is a detecting method designed by using the principle that the hoptoglobin is a human protein, has antigenicity and generates cohesion after combining with an antigen antibody. The hoptoglobin in the serum sample is combined with adequate specific antibody in the agent to form an insoluble immune complex; the change of the absorbance of the reaction solution is relevant to the content of the hoptoglobin in the serum sample. The method for detecting the hoptoglobin in the serum and the detecting kit in the invention have the advantages that: the serum sample is not subjected with special treatment, and can be ordinarily detected on an automatic biochemistry analyzer; the result directly reflects the true protein content of the hoptoglobin in the serum sample; therefore, the invention is used for clinic detection.

The invention discloses a fluorescent probe for detecting microcystic toxin-LR in water, which belongs to the field of molecular biology and microbiology. According to the fluorescent probe, MC-LR is taken as a target, a dodecapeptide library randomly shown on the surface of a bacteriophage is used for selecting a recombination bacteriophage which is combined with the MC-LR in a specific manner, and the single-stranded DNA (Deoxyribonucleic Acid) of the recombination bacteriophage is extracted and recombined for sequencing and sequence alignment, thereby obtaining an MC-LR specific affinity ligand sequence: His-Phe-Phe-Lys-Trp-His-Thr-Arg-Thr-Asn-Asp-Gln, and subsequently, obtaining the specifically combined fluorescent probe FITC-Acp-His-Phe-Phe-Lys-Trp-His-Thr-Arg-Thr-Asn-Asp-Gln through solid-phase polypeptide synthesis and fluorescent marks. The fluorescent probe has the beneficial effects that firstly, a completely new tool is provided for the specific identification and content detection of the MC-LR; and secondly, the fluorescent ligand probe can be used for qualitatively identifying the MC-LR and quantitatively detecting the content of the MC-LR in a sample.

The invention provides a method for quickly detecting the quality of sugar cane juice by using characteristic information of quality differences consisting of sugar degree, concentration (brix) and cane sugar content of the sugar cane juice, reflected in a near infrared spectrum. The method comprises the following steps of: crushing a sugar cane sample, squeezing and filtering to obtain a sugar cane sample; acquiring a near infrared spectrum of the sugar cane sample; and converting acquired near infrared spectrum information into quality parameters of the sugar cane juice to be detected with a partial least square algorithm by using the characteristic information reflected by different quality parameters of the sugar cane juice in the near infrared spectrum to quickly detect the quality of the sugar cane juice. Due to the adoption of the method, pricing by quality is conveniently realized for a sugar making enterprise, the production consumption is lowered, excellent quality and high price during selling can be practically and technically ensured for a sugar cane peasant, and the benefits of the sugar cane peasant are guaranteed.

The invention discloses a quick detector for quality and safety of tea. The detector is provided with a machine shell of which the upper surface is provided with a color comparison cuvette cover. Thedetector is characterized in that: a transmission type color comparison cuvette and a reflection type color comparison cuvette are arranged side by side under the color comparison cuvette cover of themachine shell; the transmission type color comparison cuvette is a rectangular tank body, one side surface of the tank body is provided with a light source / monochromator, and the other opposite sidesurface is provided with a photoelectric detector; and the reflection type color comparison cuvette is a cuboid of which the inside is provided with three T-shaped channel holes, channel openings of two side channel holes are provided with a light source / monochromator respectively, the lower part channel opening of the middle channel hole is provided with a photoelectric detector, and the lower part channel opening of the middle channel hole is provided with a chromatographic plate. The detector can be used for quickly and quantitatively detecting the contents of heavy metal and pesticide in tea in field and quickly detect the quality safety of tea in field.

The invention discloses a fluorescent probe for detection of cystatin C and a construction method thereof, relates to the molecular biological and microbiological technical fields, and is characterized in that the construction method comprises the steps: with Cys-C as a target, screening to obtain a Cys-C specific-binding recombinant phage by using a phage surface random displayed 12-mer peptide library, extracting single stranded DNA of the recombinant phage, carrying out sequencing and sequence comparison, to obtain a sequence Gln-Val-Asn-Gly-Leu-Gly-Glu-Arg-Ser-Gln-Gln-Met of a Cys-C specific affinity ligand having the molecular weight of 1346.5, then carrying out solid phase polypeptide synthesis and fluorescence labeling to obtain the fluorescent probe FITC-Acp-Gln-Val-Asn-Gly-Leu-Gly-Glu-Arg-Ser-Gln-Gln-Met having the molecular weight of 1848.7 and specifically bond with the Cys-C. The fluorescent probe and the construction method thereof have the beneficial effects: 1, a brand new 'tool' is provided for specific identification and content detection of the Cys-C; and 2, the fluorescent ligand peptide probe not only can qualitatively identify the Cys-C, but also can quantitatively detect the content of the Cys-C in samples.

The invention relates to the field of cyanide detection, in particular to a method of ultrasensitive detection on cyanide based on a nanogold substrate SERS technology. The method comprises the following steps: (1) mixing cyanide solutions with different concentrations and aqueous solutions with different concentrations with a nanogold detection substrate respectively to obtain multiple groups of mixed solutions, determining an SERS strength value of each group of the mixed solution, and then drawing a standard curve graph of the cyanide concentrations and the SERS strength value; and (2) mixing the solution to be detected with the nanogold detection substrate to obtain a mixed solution to be detected, determining an SERS strength value of the mixed solution to be detected, and then obtaining the concentration value of the cyanide from the curve graph. The detection method has the advantages that is simple to operate and low in cost, can rapidly and sensitively detect the cyanide, can rapidly detect the cyanide with high specificity and high sensitivity below a lower limit of detection and can still efficiently detect the cyanide to be detected under the condition that the concentration of an interferent reaching up to tens times of the cyanide to be detected.

The invention discloses a composition and a kit for detecting the content of donkey hide source component in tortoise shell gelatin, deer horn gelatin and fake gelatin, and a detection method thereof. The composition comprises polypeptide I, polypeptide II, polypeptide III and control detection matrix, wherein the amino acid sequence of the polypeptide I is shown as SEQ ID NO.1, the amino acid sequence of the polypeptide II is shown as SEQ ID NO.2, the amino acid sequence of the polypeptide III is shown as SEQ ID NO.3, and the control detection matrix is fishskin gelatin. The detection value of the polypeptide II is subtracted from the detection value of the polypeptide I, and the detection deviation can be corrected by utilizing the detection value of the polypeptide III, so that the detection of content of donkey hide source component in the tortoise shell gelatin, deer horn gelatin and fake gelatin can be realized. The method has the advantages of being accurate in content detection, and simple to operate, and has wide application prospects on the true and false identification aspect of tortoise shell gelatin, deer horn gelatin and fake gelatin.

The invention provides a method for detecting glyceryl monostearate in liquid milk. The method comprises the following steps: a. preparing a bottle of liquid milk to be tested, extracting milk fat from the liquid milk so as to obtain milk fat to be tested; b. preparing a standard solution of glyceryl monostearate, blow-drying the standard solution with nitrogen gas so as to obtain a reference substance; c. separately adding N,O-bis(trimethylsilyl)trifluoroacetamide into the milk fat to be tested and the reference substance to carry out derivatization reactions so as to separately obtain a derivative sample and a derivative reference substance; d. blow-drying the derivative sample and the derivative reference substance with nitrogen gas, then separately dissolving the derivative sample and the derivative reference substance with n-hexane so as to separately obtain a sample liquid to be tested and a reference detection liquid; e. measuring the gas chromatography values of glyceryl monostearate in the sample liquid to be tested and the reference detection liquid, and calculating according to the gas chromatography values of glyceryl monostearate in the sample liquid to be tested and the reference detection liquid so as to obtain the content of glyceryl monostearate in the liquid milk to be tested. The detection method has the advantages of convenient operation and high accuracy.

The invention provides a method for determining an aminoglycoside drug in an animal muscle tissue without using of an ion pair reagent, and belongs to the technical field of veterinary drug residue detection, and the method comprises performing liquid chromatography-tandem mass spectrometry detection on an injection sample, and obtaining the content of the aminoglycoside drug in the injection sample according to a standard curve of the content of the aminoglycoside drug and a liquid chromatogram of the injection sample; wherein a liquid chromatography column is SIELC Obelisc R. The method provided by the invention can enhance the chromatographic retention without using of the ion pair reagent, and can detect the content of the aminoglycoside drug the animal muscle tissue, the detection limit and the limit quantification of the drug are respectively in a range of 1 to 10 mu g / kg and a range of 2-20 mu g / kg; within-run precision and between-run precision are 2-14.4% and 3.7-19.8% respectively.

The invention provides a filtering device for a painting room. The filtering device comprises at least one group of filtering single pieces which communicates with the waste gas outlet of the painting room or at least two groups of filtering single pieces which are arranged in parallel, wherein each filtering single piece comprises a bag dedusting component, an active carbon adsorption component, an exhausting component and a parameter measuring component; each bag dedusting component comprises a bag dedusting body, and an inlet and an outlet formed on the bag dedusting body; the active carbon adsorption component comprises a shell and an active carbon adsorption layer which is detachably arranged on the middle part of the shell; the shell is partitioned into an upper cavity and a lower cavity which are arranged separately by the active carbon adsorption layer; the upper cavity is provided with an airflow outlet communicating with the exhausting component; the lower cavity communicates with the outlet; the parameter measuring component comprises a pressure difference measuring single piece and a hydrocarbon organic matter detection single piece; the two ends of the pressure difference measuring single piece are arranged on the inlet and the outlet respectively; the hydrocarbon organic matter detection single piece is arranged at the air outlet of the exhausting component. The filtering device has a simple structure and a good dedusting effect.

The invention relates to preparation of chicken feather nitrogen-doped carbon quantum dots and a fluorescent probe and a paraquat detection method. The method comprises the following steps of: uniformly mixing a chicken feather material and deionized water according to a mass-volume ratio of (0.2-1.5)g: 60mL; adding 0.2 mL-3 mL of a nitrogen-containing solution into the solution to serve as a doping agent, conducting ultrasonic dispersion for 2 min-10 min so as to obtain a to-be-reacted solution; putting the to-be-reacted solution into a closed reaction kettle, performing a reaction for 1.5-48hours at the temperature of 100-300 DEG C to obtain a hydrothermal reaction product solution, naturally cooling the solution, and taking out the solution; centrifuging the solution, taking the supernatant of a centrifuged brown yellow liquid, and filtering the supernatant to obtain a chicken feather nitrogen-doped carbon quantum dot solution. A corresponding fluorescent probe is obtained throughthe chicken feather nitrogen-doped carbon quantum dot solution; the excitation wavelength and emission wavelength are explored, a linear regression equation is fitted through a standard solution, anddetection is completed through the equation. The environment-friendly waste biomass material is used as the raw material, the biomass atom doped fluorescent carbon quantum dots are prepared through the one-step hydrothermal method; and paraquat content can be detected through a provided ''switch'' type N-CQDs / Hg <2+> fluorescent probe solution.

The invention discloses a formaldehyde electrochemical detection system. According to the formaldehyde electrochemical detection system, one end of a first connection channel is communicated with a first container; one end of a second connection channel is communicated with a second container; the other end of the first connection channel is communicated with the other end of the second connection channel by an ion exchange membrane; a working electrode and a reference electrode are located in the first container; a counter electrode is located in the second container; a communication port of the working electrode is connected with a current positive electrode measuring control port of a signal conditioning circuit; the commutation port of the counter electrode is connected with a current negative electrode measuring control port of the signal conditioning circuit; the commutation port of the reference electrode is connected with an electric potential state stable port of the signal conditioning circuit; the signal output end of the signal conditioning circuit is connected with the signal input end of a signal processor. According to the formaldehyde electrochemical detection system, a good linear relation between an electrolytic current signal and the concentration of formaldehyde is used so that the formaldehyde electrochemical detection system can be used for fast detecting the content of the formaldehyde in foods; the trace formaldehyde in the foods can also be detected.

The invention provides a preparation method of high-purity 3-deacetylcephalosporin C sodium salt. The preparation method comprises the following steps: (1) dissolving; (2) cracking; (3) crystallizing;and (4) filtering and drying. According to the preparation method, local peracid in the crystallization process can be avoided, degradation is reduced, the usage amount of concentrated hydrochloric acid and ammonia water is reduced, obvious economic benefits are achieved, meanwhile, erosion of chloride ions to production equipment is reduced, loss of the equipment is reduced, the volume of a mother liquor can be reduced, and the yield is increased. The purity of the 3-deacetylcephalosporin C sodium salt prepared by the preparation method disclosed by the invention is greater than 99% of DCPC;when the3-deacetylcephalosporin C sodium salt is used as a reference substance, the content of DCPC can be more accurately detected, the content of DCPC can be better controlled and reduced in CPC, DAOC and 7-ACA and 7-ADCA production, and the product quality is improved.

The invention discloses a sulfite detection reagent and a detection method thereof. The reagent comprises the following ingredients: 5,5-dithio-bis(2-nitrobenzoic acid), sodium tetrachloromercurate, pararosaniline hydrochloride, acetic acid, barium chloride, foamed aluminium, sodium bicarbonate, chitin, sodium molybdate, formaldehyde, glycerin, zinc acetate and water. The detection method comprises the following process: crushing a sample when the sample to be tested is a solid and then adding deionized water, decocting with slow fire, filtering and taking a filtrate, adding 1-3 droplets of the sulfite detection reagent into the filtrate, standing for 1-3 min, and observing color change (the solution turns amaranth when concentration value of sulfite is higher than 0.08 microgram / g; and the solution will not change color when concentration value of nitrite is lower than 0.08 microgram / g); and directly dropwise adding the sulfite detection reagent if the sample to be tested is a liquid.The test paper can rapidly detect content of sulfite in food, is convenient to transport and carry, and is convenient to operate.

The invention provides a method for rapidly measuring the content of furfural by a high performance liquid chromatography. A chromatographic column adopted by the method is a polystyrene divinylbenzene resin chromatographic column; a mobile phase of the column is a sulfuric acid solution; the concentration of the sulfuric acid solution is 0.001-0.01mol / L; the detection temperature of the column is 55-65 DEG C; and the temperature of a differential detector is 40-55 DEG C. The method is high in measuring speed, can rapidly measure the content and the concentration of furfural in aldehyde steam, and can be used for semifinished product production control, and for guiding judgment of a reaction end point in a dehydration and cyclization technological process.

The invention discloses a method for detecting dehydrogenized vitamin C in vitamin C and belongs to the field of analytic chemistry. The method is characterized in that the method uses liquid chromatography and an HILIC affinity chromatography column and uses acetonitrile--isopropanol-water as the mobile phase A and acetonitrile-water as the mobile phase B to perform analysis through gradient elution. The method has the advantages that by the method, the content of the dehydrogenized vitamin C can be analyzed fast and accurately; the quantification limit of the method can reach 0.38 microgram / ml, the detection limit of the method is 0.11 microgram / ml, the average yield of the method is 100.5%, and the concentration of the dehydrogenized vitamin C and peak area present a good linear relation in the range from 0.38 microgram / ml to 77.75 microgram / ml; the liquid-phase method is also applicable to vitamin quality researches such as researches on the stability and degradation of vitamin C medicine, compound vitamin (3) for injection and compound vitamin (13) injection.

The invention provides a method for detecting purithiamine in blood by efficient liquid chromatography. The method is characterized in that the method comprises the specific steps: taking whole blood, removing protein, and using the efficient liquid chromatography to detect, wherein the condition of the chromatography comprises: using C18 chromatographic column; taking disodium hydrogen phosphate solution and monosodium orthophosphate obtained in step 1 as a first mobile phase and a second mobile phase respectively; and gradually eluting at the flow speed of 1.0 mL / min, wherein the exciting wavelength of fluoroscopic detection is 385nm and the emitting wavelength is 460nm. The invention has the advantage that the content of the purithiamine in the blood can be detected by a simple method.

The invention discloses a hydrogen cyanide detection reagent and a preparation method thereof and belongs to the technical field of gas detection. The reagent includes the components including, by weights: 3 to 6 parts of sodium hydroxide, 5 to 10 parts of phenol resin, 3 to 6 parts of aceton, 9 to 19 parts of helianthin B, 3 to 7 parts of chrysolepic acid, 2 to 5 parts of polyacrylate, 6 to 11 parts of ethanol and 10 to 15 parts of distilled water. The method includes the steps of 1, preparing a premix liquid; 2, obtaining a clear premix liquid; 3, standing for 12 to 48 hours in vacuum, sealing, and obtaining the hydrogen cyanide detection reagent. The obtained hydrogen cyanide detection reagent has the advantages of high reaction flexibility and accuracy, the content of hydrogen cyanide can be detected effectively, and the damage can be reduced.

The invention provides a method for detecting 2, 4-dichlorphenoxyacetic acid and belongs to the technical field of pesticide detection. The method comprises steps of an alkaline phosphatase solution and a sample being mixed and left to stand, and standing liquid being obtained; mixing the standing liquid with a sodium pyrophosphate solution and a Tris-HCl buffer solution, and carrying out a reaction to obtain a reaction liquid; mixing the reaction solution with a cerous nitrate solution, oscillating to obtain an oscillating solution, measuring the fluorescence emission spectrum of the oscillating solution to obtain the maximum fluorescence intensity, and converting the maximum fluorescence intensity into an enzyme inhibition rate; and substituting the enzyme inhibition ratio into the linear equation to obtain the concentration of 2, 4-dichlorophenoxyacetic acid in the sample. By adopting the method provided by the invention, a detection probe does not need to be prepared, and a detection result can be obtained within 45 minutes; the linear range of the linear equation is 2.5-200 [mu] g / L, and the detection limit is 0.98 [mu] g / L.

The invention belongs to the technical field of biology, and provides an anti-SpA5 protein monoclonal antibody, application thereof, and a kit containing the monoclonal antibody. The anti-SpA5 proteinmonoclonal antibody comprises a heavy chain and a light chain, wherein the heavy chain and the light chain respectively comprise three CDR regions, the amino acid sequences of the heavy chain CDR regions are respectively as shown in SEQ ID NO: 1-3, and the amino acid sequences of the light chain CDR regions are respectively as shown in SEQ ID NO: 4-6. The antibody disclosed by the invention can be specifically combined with SpA5 protein and effectively detect the content of the SpA5 protein, and has high sensitivity and wide detection range.

The invention discloses application of a product for detecting a gas production rate of neonatal intestinal flora in preparation of a product for predicting, diagnosing or assisting diagnosis of neonatal NEC. The product for detecting the gas production rate of the neonatal intestinal flora further comprises prebiotics for fermenting excrement samples of newborns. According to the application of the product, the excrement suspension of a subject is directly inoculated into a culture medium for in-vitro fermentation, so that the gas components are detected, and the content of gas produced by microorganisms at the colon part of the subject, the composition components and the proportion of each component can be more accurately detected. Meanwhile, markers related to the necrotizing enterocolitis of newborns are found, and a new way is provided for prediction, diagnosis or auxiliary diagnosis of the newborn necrotizing enterocolitis.

The invention discloses a full-range C-reactive protein detection kit, which comprises a reagent R1 and a reagent R2. The reagent R1 is a proper buffer solution; the reagent R2 is formed by the following steps: sensitizing two or more styrene latex with different average grain diameters by using an anti-human C-reactive protein antibody; placing the sensitized styrene latex with the different average grain diameters into the proper buffer solution respectively to form reagents; and finally mixing the reagents in different proportions, wherein the reagent R2 contains 0.8 to 3.5 mg / mL of the styrene latex combined with the anti-human C-reactive protein antibody. The full-range C-reactive protein detection kit not only can measure lower CRP content but also can measure high CRP content, and has the advantages of high sensitivity and stability and accurate measurement.

The present invention discloses a method for separating components in a mixture by use of an elisa plate and a monoclonal antibody. The separation method comprises the following steps: the elisa plate is coated with an antibody of a component A in the mixture, substances containing the component A in the mixture to be tested are bonded to form an antigen-antibody complex immobilized on the plate, supernatant contains no A component, so that components excluding the component A can be separated out. The method is more precise and specific for separating the components. Only one same detection system can separate and quantitatively determine different substances to be determined containing one same component. The volume of the needed sample to be tested is greatly reduced from milliliters to microliters; complicated equipment investments are not required, testing cost is reduced, testing time is greatly shortened from 3 to 4 days to 3 to 4 hours, and detection accuracy is greatly increased from micrograms to nanograms.

The invention belongs to the technical field of quality control, and particularly relates to a method for determining the content of garlicin diallyl trisulfide in garlicin tablets. The method comprises the steps as follows: a to-be-detected sample of the garlicin tablets is mixed and dissolved with N, N-dimethylformamide, detection with high performance liquid chromatography and analysis with an external standard method are performed, and the content of diallyl trisulfide is obtained. With the adoption of the detection method, diallyl trisulfide can be fully separated from the garlicin tablets, and accordingly, the content of diallyl trisulfide in the garlicin tablets can be accurately detected. The detection method has the advantages of good linearity, repeatability and stability, high precision, low detection limit and quantification limit and high recovery rate, and blanks basically have no interference to determined results.

The invention relates to a transmission-type optical detection device for an intelligent expiration molecular diagnosis system. The detection device comprises a reaction tank, a biologic sensor and a light path structure for spectral analysis, wherein the biologic sensor comprises a reactant which can react with and absorb a biomarker exhaled by a human body; a reaction chamber is arranged in the reaction tank; the reaction chamber is communicated with an external human exhaled air source; the biologic sensor is arranged in the reaction chamber and the reactant is packaged in the reaction chamber; the light path structure can generate light beam and inject into the reaction chamber; after the light beam passes by the reactant, the light beam is emitted out from the reaction tank, so as to form an absorption spectrum of the reactant. The transmission-type optical detection device for the intelligent expiration molecular diagnosis system adopts the reactant of the biologic sensor for reacting with the biomarker exhaled by the human body, the light path structure for performing spectral analysis on the reactant is adopted for detecting the reactant after absorbing the biomarker, the content of the biomarker exhaled by the human body can be quickly and accurately detected and the precision is high.

The invention provides environment-friendly intelligent septic tank purification equipment and a purification method thereof. The equipment comprises a vehicle body, a vacuum tank, a vacuumizing device, a sewage inlet pipe, a sewage pump, a filter press, a clear water tank, a sewage pumping pipe, a rotary solid-liquid separator, a garbage chamber, an agent feeding tank, a detection device, a purification device and a two-way water pump. The vacuum tank is mounted on the upper surface of the vehicle body through bolts, and the vacuumizing device is mounted at the right end of the vacuum tank atthe bottom of the vehicle body through bolts. The rotary solid-liquid separator is mounted on the inner right side of the vacuum tank through bolts, separation of large solid impurities and liquid can be realized beneficially, the large solid impurities are sent into the garbage chamber, and liquid substances enter a press filter through the sewage inlet pipe to realize solid-liquid separation. The two-way water pump is mounted at the outer upper end of the vacuum tank through bolts and connected with the clear water tank and the purification device, sewage treatment conditions in a detectiontank can be well detected, the two-way water pump is available for recovery treatment, and high efficiency, quickness and intelligence in a whole treatment process are realized.

The invention provides a method for detecting active metal elements in hard zinc. The method comprises the following steps that 1, roasted or oxidized extracted hard zinc slag is subjected to ball milling and sifting through a 50-150 mesh sieve; 2, the sifted hard zinc slag is added to a copper sulfate solution for heating, reaction and filtering to obtain a filtrate and filter slag, wherein the content of copper in the copper sulfate solution is 50-10000 ppm, and the mass ratio of the copper sulfate solution to the sifted hard zinc slag is (3-20): 1; 3, the content of copper in the filtrate is detected to obtain the content of active metals in the hard zinc slag. The method utilizes the reaction of the active metals with copper sulfate to cause the change of the copper content, calculatesthe content of the active metals in the roasted or oxidized hard zinc slag, and can quantitatively detect the content of the active metal elements in the slag; the method can not only quantify, but also can detect slag with the active metal content of 10 ppm, and solves the difficult problem of quantitative detection of trace active metal elements in solid slag.