Composition and kit for detecting the content of donkey hide source component in tortoise shell gelatin, deer horn gelatin and fake gelatin, and detection method thereof

A technology of deer antler glue and tortoise shell glue, which is applied in the direction of measuring devices, material separation, and material analysis, can solve the problem that the components derived from donkey skin do not exclude the components derived from horse hide, cannot be quantitatively detected, and cannot be relatively stable, etc. question

Active Publication Date: 2017-06-13
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both of the reported methods are unable to carry out relatively accurate quantitative detection of donkey skin-derived components in gelatinous traditional Chinese medicines such as donkey-hide gelatin, tortoise shell glue, and deer horn glue, because the selected specific ingredients often change with the process and cannot be relatively stable. Moreover, the detection of ingredients derived from donkey skin basically did not rule out ingredients derived from horse hide. Therefore, although it is claimed to be derived from donkey skin, it is actually shared by donkeys and horses.

Method used

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  • Composition and kit for detecting the content of donkey hide source component in tortoise shell gelatin, deer horn gelatin and fake gelatin, and detection method thereof
  • Composition and kit for detecting the content of donkey hide source component in tortoise shell gelatin, deer horn gelatin and fake gelatin, and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Detection of pure glue

[0035] 1. Materials and reagents

[0036] Material: all kinds of pure glue, including donkey skin, horse skin, and cowhide skins, including donkey skin, horse skin, and cowhide skins, including donkey skin glue, horse skin glue, yellow gelatin, pig skin glue, sheep skin glue, tortoise shell glue, antler glue, and fish skin glue , Pigskin, sheepskin, tortoise shell, antlers and fish skins are decocted, concentrated and dried.

[0037] Polypeptide I (shown in SEQ ID NO. 1), polypeptide II (shown in SEQ ID NO. 2), and polypeptide III (shown in SEQ ID NO. 3) were synthesized by a biological company.

[0038] Reagents: ammonium bicarbonate (analytical purity), trypsin (sequence purity).

[0039] 2. Detection method

[0040] 1) Preparation of matrix solution: Weigh 0.50g of the reference substance test matrix into a 250ml measuring flask, and add a small amount of 1% NH 4 HCO 3 Solution (pH8.0), ultrasonic to completely dissolve the sample, use 1% NH ...

Embodiment 2

[0057] Example 2 Detection of mixed glue samples

[0058] 1. Materials and reagents

[0059] Material: The mixed gum sample is made from the tortoise shell gum and the pure product gum sample of deer horn gum in Example 1 by adding different quality donkey-hide glue, including tortoise shell glue containing 15% (w / w) donkey-hide glue, 30% (w / w) ) The tortoise shell glue of Ejiao and the deerhorn glue containing 30% (w / w) of Ejiao were used as samples 1-3, respectively.

[0060] Polypeptide I (shown in SEQ ID NO. 1), polypeptide II (shown in SEQ ID NO. 2), and polypeptide III (shown in SEQ ID NO. 3) were synthesized by a biological company.

[0061] Fish skin glue (reference substance detection matrix) was prepared according to the method described in Example 1.

[0062] Reagents: ammonium bicarbonate (analytical purity), trypsin (sequence purity).

[0063] 2. Detection method

[0064] 1) Preparation of matrix solution: Weigh 0.50g of the reference substance test matrix into a 250ml measu...

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Abstract

The invention discloses a composition and a kit for detecting the content of donkey hide source component in tortoise shell gelatin, deer horn gelatin and fake gelatin, and a detection method thereof. The composition comprises polypeptide I, polypeptide II, polypeptide III and control detection matrix, wherein the amino acid sequence of the polypeptide I is shown as SEQ ID NO.1, the amino acid sequence of the polypeptide II is shown as SEQ ID NO.2, the amino acid sequence of the polypeptide III is shown as SEQ ID NO.3, and the control detection matrix is fishskin gelatin. The detection value of the polypeptide II is subtracted from the detection value of the polypeptide I, and the detection deviation can be corrected by utilizing the detection value of the polypeptide III, so that the detection of content of donkey hide source component in the tortoise shell gelatin, deer horn gelatin and fake gelatin can be realized. The method has the advantages of being accurate in content detection, and simple to operate, and has wide application prospects on the true and false identification aspect of tortoise shell gelatin, deer horn gelatin and fake gelatin.

Description

Technical field [0001] The invention relates to a composition and a kit for detecting the content of donkey skin source components in tortoise shell glue, deer horn glue, donkey-hide glue and its fake glue, and also includes using the composition or kit to perform tortoise shell glue, antler glue, and donkey-hide glue The present invention belongs to the technical field of medicine and food detection technology. Background technique [0002] As a traditional precious Chinese medicine, donkey-hide gelatin has a history of consuming thousands of years. It has the effects of nourishing blood, nourishing yin, moisturizing dryness, and stopping bleeding. According to the Chinese Pharmacopoeia, donkey-hide gelatin is a solid glue made by decoction and concentration of the dried skin or fresh skin of the equine donkey Equus asinus L.. Pure donkey-hide gelatin must come from 100% donkey skin to ensure the effectiveness of the product. From the perspective of raw material taxonomy, donk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 段小波郭尚伟郝向慧徐云鹏王静王玉娇石永坚周祥山藏香银
Owner SHAN DONG DONG E E JIAO
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