Method for detecting purithiamine in blood by efficient liquid chromatography
A high-performance liquid chromatography and pyridinethiamine technology, applied in the field of biomedicine, can solve problems such as abnormal thiamine metabolism and thiamine deficiency
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[0022] Example 1
[0023] A method for detecting the content of pyrithione in blood by high performance liquid chromatography:
[0024] Step 1: Store the blood at -80°C; add potassium ferricyanide to 150g / L sodium hydroxide solution to prepare 0.25g / L potassium ferricyanide solution; add pyrithione to 0.1M hydrochloric acid solution Prepare a 0.1 mM pyrithione solution, store it in the dark at 4°C, dilute it with a methanol solution with a weight concentration of 20% on the day of the measurement to prepare a 100 nM pyrithione solution, set its concentration as Cs; add disodium hydrogen phosphate to the weight concentration Prepare 25mmol / L disodium hydrogen phosphate solution in 10% methanol solution; add sodium dihydrogen phosphate to 70% methanol solution by weight to prepare 25mmol / L sodium dihydrogen phosphate solution;
[0025] The second step: mix the pyridinethiamine solution obtained in the first step with the potassium ferricyanide solution in a volume ratio of 25:3, fil...
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[0029] Example 2
[0030] Another method to detect the content of pyrithione in blood by high performance liquid chromatography:
[0031] Step 1: Store the blood at -80°C; add potassium ferricyanide to 150g / L sodium hydroxide solution to prepare 0.25g / L potassium ferricyanide solution; add pyrithione to 0.1M hydrochloric acid solution Prepare a 0.1 mM pyrithione solution, store it in the dark at 4°C, dilute it with a methanol solution with a weight concentration of 20% on the day of the measurement to prepare a 100 nM pyrithione solution, set its concentration as Cs; add disodium hydrogen phosphate to the weight concentration Prepare 25mmol / L disodium hydrogen phosphate solution in 10% methanol solution; add sodium dihydrogen phosphate to 70% methanol solution by weight to prepare 25mmol / L sodium dihydrogen phosphate solution;
[0032] The second step: mix the pyridinethiamine solution obtained in the first step with the potassium ferricyanide solution in a volume ratio of 25:3, f...
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