7results about How to "Rapid Quantitative Detection" patented technology

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.

This invention belongs to the field of fetoglobulin B detection technology, and discloses an immunochromatographic test strip for quantitative detection of fetoglobulin B using quantum dot fluorescent microspheres and its preparation method. The immunochromatographic test strip of this invention includes a sample pad, a release pad, and a nitrocellulose membrane; the release pad is coated with a fetoglobulin B monoclonal antibody labeled with quantum dot fluorescent microspheres and a mouse IgG antibody labeled with quantum dot fluorescent microspheres; the nitrocellulose membrane has a detection line and a control line, the detection line is coated with fetoglobulin B monoclonal antibody, and the control line is coated with goat anti-mouse IgG antibody. The immunochromatographic test strip of this invention reduces the detection time to 9-12 minutes, and the total detection time is only 20 minutes, achieving rapid and accurate quantitative detection of fetoglobulin B. It also features high detection sensitivity, a wide detection range, and simple and rapid operation, which is beneficial for the screening and monitoring of atherosclerotic coronary heart disease.

The present application belongs to the field of health quarantine, and relates to a borrelia burgdorferi rapid detection kit and a detection method. The kit can perform on-site rapid quantitative detection on a sample to be detected based on fluorescence microsphere immunochromatography technology. The kit comprises a borrelia burgdorferi fluorescence microsphere immunochromatography test strip. The test strip comprises a viscous bottom liner and viscous bottom plate, and is sequentially pasted with an analysis membrane, a binding pad, a water absorption pad and a sample pad. The analysis membrane is a nitrocellulose membrane, which has a detection band and a quality control band, and is respectively fixed with borrelia burgdorferi antigen protein and rabbit anti-SPA. The binding pad is fixed with an SPA and fluorescence microsphere labeled compound. The present application can realize on-site rapid quantitative detection of borrelia burgdorferi carried by mice, and has the advantages of simple operation, short detection time, high detection sensitivity, good specificity, convenient storage and easy popularization and use.

The invention relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disruption activity in sewage and application. A Y-type polypeptide containing an anchoring region, a hinge region and an antifouling region is creatively designed as a structural skeleton, and the tail ends of a main chain and a branch chain are respectively subjected to directional modification to prepare the multifunctional ligand-polypeptide-dye conjugate; an optical biosensing chip, namely a ligand-polypeptide-dye conjugate functionalized biosensing chip which is high in reaction efficiency, strong in anti-pollution characteristic and capable of being stably regenerated, is constructed by adopting a covalent coupling method, and then the quantum dot-nuclear receptor fluorescent probe which is high in sensitivity and adjustable in emission spectrum is prepared. The ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is provided, rapid quantitative detection of the endocrine disruptors in sewage is achieved, and the endocrine disruptors have broad-spectrum specific recognition capacity on a certain type of endocrine disruptors.

This invention discloses a method for preparing a plasmonic-enhanced fluorescence-coupled aptamer ratio sensor based on gold nanoparticles and its application in the visual detection of amyloid protein. The invention involves preparing gold nanoparticles and immobilizing them on an amino-modified ELISA plate by adding EDC / NHS. β-amyloid aptamers, with amino and thiol groups modified at both ends respectively, are grafted onto the gold nanoparticle-modified ELISA plate via Au-S bonds. EDC / NHS is then added to graft Rosa Bengal onto the aptamers on the gold nanoparticles via amide bonds, resulting in a plasmonic-enhanced fluorescence-coupled aptamer as the responsive fluorescent probe and 9-anthracarboxylic acid as the reference fluorescence, thus creating a plasmonic-enhanced / ratio-fluorescent probe sensor. The plasmonic-enhanced / ratio-fluorescent probe sensor prepared by this invention exhibits good stability, enabling portable, ultrasensitive, and specific visual quantitative detection of β-amyloid protein.

This invention discloses a method for synthesizing a standard sample for detecting tartrazine intermediates as shown in structural formula 1, characterized by the following reaction formula 1. This patent provides a method for preparing a standard sample for detecting the content of tartrazine intermediates, the structure of which is shown in structural formula 1, enabling rapid and accurate quantitative detection of 1-(4'-sulfonylphenyl)-3-carboxylic acid methyl ester-5-pyrazolone monosodium salt in tartrazine.

The present application relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disrupting activity in sewage and application. The present application creatively designs a Y-shaped polypeptide containing an anchoring region, a hinge region and an anti-fouling region as a structural framework, and performs directional modification at the ends of the main chain and the branch chain to prepare a multifunctional ligand-polypeptide-dye conjugate; a covalent coupling method is used to construct a high-response-efficiency, strong-anti-pollution-characteristic and stable-regeneration optical biosensor chip, i.e. a ligand-polypeptide-dye conjugate functionalized biosensor chip, then a quantum dot-nuclear receptor fluorescence probe with high sensitivity and adjustable emission spectrum is prepared, a ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is proposed, and rapid quantitative detection of endocrine disruptors in sewage is realized, so that the present application has broad-spectrum specific recognition ability for certain endocrine disruptors.