309results about How to "Rapid Quantitative Detection" patented technology

The invention in particular relates to a whole-blood labeled immunoassay method for quickly collecting a target object from whole-blood through specificity binding reaction and labeling and monitoring the target object by a labeling technology, and an instant detection system. The method comprises the following steps: 1) capturing magnetic beads; 2) eluting blood; 3) labeling a detection antibody; 4) eluting the detection antibody; 5) detecting. The system comprises a microfluidic chip, a magnetic field generation device arranged above or below the microfluidic chip and a detection device, wherein the microfluidic chip comprises a microchannel, the magnetic beads arranged in the microchannel, a waste liquid outlet and an eluent storage pond; the microchannel is separated into a first reaction region, a second reaction region and a detection region in stages by a separation mechanism. The technical scheme provided by the invention has the characteristics of high sensitivity, high repeatability, simple system structure, low cost and the like, and can detect the target object from trace blood samples quickly and quantitatively.

The invention discloses a fluorescent microsphere immunochromatographic testing card for testing five indexes of hepatitis b and a method for preparing the same. The testing card comprises a hepatitis b surface antigen test paper strip, a hepatitis b e surface antigen test paper strip, a hepatitis b surface antibody test paper strip, a hepatitis b e surface antibody test paper strip, and a hepatitis b core antibody test paper strip. Each test paper strip is formed by overlapping and bonding filter paper, a sample pad, a glass fiber film spray-coated with fluorescent microspheres, a cellulose nitrate film and water absorption paper on a bottom plate by glue in sequence, wherein the cellulose nitrate film is coated with antigens serving as a testing area and anti-rabbit antibodies serving as a quality control area; and during a test, after emitted fluorescent light passes a filter, the emitted spectrum is collected, accumulated and multiplied by the CCD scanning technology and then converted into a numerical signal, the numerical signal is multiplied by a correction factor, and the strength of the corrected fluorescent light is substituted in a standard curve of a fluorescence analyzer, so that the concentrations of the five indexes of hepatitis b of the sample can be automatically worked out. The test of hepatitis b viruses by the testing card has the characteristics of specificity, sensitivity, simpleness and accuracy.

The invention relates to an immunochromatography time resolution fluorescence kit for synchronously detecting mixed pollution of five types of fungaltoxinfungi toxins of aflatoxin and the like and application. The immunochromatography time resolution fluorescence kit comprises an immunochromatography time resolution fluorescence test paper strip and sample reaction bottles of monoclonal antibody freeze-drying samples containing europium labels, wherein the fluorescence test paper strip comprises a PVC (polyvinyl chloride) substrate; a water absorbing pad, a detection pad and a sample pad are sequentially adhered onto one surface of the substrate; the adjacent pads are overlapped and connected at the connecting part; the detection pad uses a nitrocellulose membrane as a base pad, and is provided with a transverse quality control line and five detection lines from top to bottom to respectively coat the bovine serum albuminaflatoxin B1-BSA conjugate of each toxin; a fumonisin B1 monoclonal antibody is excreted by a hybrid tumor cell strain Fm7A11 with collection number of CCTCC NO.C201636. The immunochromatography time resolution fluorescence kit is applied to synchronously detect the mixed pollution of toxins of aflatoxin B1, fumonisin B1, ochratoxin A, zearalenone and aspergillus versicolor, and has the advantages that the operation is simple and quick, and the sensitivity is high.

The invention discloses a method for detecting miRNA (ribonucleic acid). The method comprises the steps: (1) synthesizing a DNA (deoxyribonucleic acid) tetrahedron probe; (2) connecting three peak points of the DNA tetrahedron probe to the surface of a working electrode of an electrochemical device to obtain a working electrode with a capture probe; (3) adding a target miRNAs to be detected, a signal probe and an auxiliary chain into a reaction system to implement hybridization reaction to form a composite body I, and immersing the working electrode into the reaction system to implement hybridization reaction to form a composite body II; (4) generating reaction between the composite body II and enzyme capable of generating catalytic oxidation reduction reaction; (5) adding a primer produced by the enzyme catalytic oxidation to realize electrochemical detection analysis. The target miRNAs can be directly detected by the method disclosed by the invention without being marked and subjected to pre-PCR (polymerase chain reaction) multiplication; the method is easy to operate, so that the experiment cost is greatly reduced, and the experiment efficiency is improved.

The invention discloses a preparation method for a paper-based micro-fluidic chip. The trimethoxy silane is processed in a heptane solution to obtain a hydrophobic base. Meanwhile, patterns printed on the channel of a piece of filter paper are cut, and then are immersed in cetyl trimethyl ammonium bromide to obtain a hydrophilic base. The above hydrophobic base and the above hydrophilic base are bonded together to form a hydrophilic and hydrophobic access. Compared with the traditional method, the above preparation method is free of any expensive instrument/agent/metal mold, thus being novel, simple, quick, low in cost and short in production period. The entire preparation process can be completed within 7 minutes.

The invention discloses a novel SERS substrate-based method for quantitatively testing pathogenic bacteria, and belongs to the technical field of biological detection. Silicon dioxide treated by an immunospectral marker is coated with gold nanoparticles for quantitative test of the pathogenic bacteria. Raman spectra signal monitoring data are more stable and reliable and can be greatly repeated. The method is good in test stability, high in sensitivity, high in specificity, the detection process is simple, fast, accurate and quantitative, and a wide detection interval and a low detection limit are obtained. The method is simple in operation, an SERS immune signal probe and a capture probe can be prepared in advance, the capture probe and the signal probe only need to be sequentially added to a to-be-detected sample for reaction during operation and the test is carried out at once through magnetic separation after the reaction is completed: the collection time is 30s, and then the concentration is immediately read from a standard curve, thereby achieving fast quantitatively testing. The method can meet the requirements of food safety and environment monitoring departments, and has wide practicability.

The invention discloses a method for synchronously detecting a plurality of organophosphorus fire retardants in bottom mud, belonging to the detection field of trace amount of organophosphorus fire retardants in the environment. The method comprises the main steps of firstly, extracting a target object in a sample through an accelerated solvent extraction instrument; purifying by a gel permeation chromatography; further purifying and enriching by adopting a solid-phase small extraction column; and finally, concentrating and making up to the constant volume and detecting and quantifying by using a gas chromatograph-mass spectrometer. According to the invention, a pre-treatment method for the organophosphorus fire retardants in the bottom mud is established and optimized, the automation degree is high and the repeatability is good; the gas chromatograph-mass spectrometer is used for carrying out quantitative detection, the detection limit is low and the sensitivity is high; the detection limit to nine types of the organophosphorus fire retardants are lower than 0.340 microgram per gram. According to the method, the synchronous analysis and detection on the plurality of trace amount of organophosphorus fire retardants in a complicated environment medium, namely the bottom mud, is realized; the sensitivity and the accuracy are achieved, and the method makes up the disadvantages of the technology in the field.

The invention provides a Raman spectrum based detection method for oxidative rancidity of ganoderma lucidum spores oil. Acid value and peroxidation value of the ganoderma lucidum spores oil are taken as evaluation indexes of the oxidative rancidity degree of the ganoderma lucidum spores oil. The detection method includes taking Raman peak integral strength of 1655cm-1 in the Raman spectrum of a standard sample as a vertical coordinate and taking the acid value or the peroxidation value as a horizontal coordinate to create standard acid value and peroxidation value evaluation drawings of the ganoderma lucidum spores oil; detecting the Raman spectrum of a tested sample, and comparing a result of the Raman peak integral strength of 1655cm-1 with the standard acid value and peroxidation value evaluation drawings to acquire the acid value and the peroxidation value of the tested sample to further acquire an oxidative rancidity degree evaluation result of the tested sample of the ganoderma lucidum spores oil. The Raman spectrum based detection method has the advantages of short detection time, low sample consumption, simplicity and convenience in operation and on-site quick detection, thereby having a broad practical application prospect.

The invention provides a super-paramagnetic nanoparticle for capturing exosomes and a preparation method thereof as well as a specific exosome luminescence immune quantitative detection kit, and belongs to the technical field of exosome detection. The super-paramagnetic nanoparticle for capturing the exosomes, provided by the invention, comprises a super-paramagnetic nanoparticle base body and anexosome shared marker antibody coupled with the super-paramagnetic nanoparticle base body. The specific exosome luminescence immune quantitative detection kit provided by the invention comprises the super-paramagnetic nanoparticle for capturing the exosomes, an exosome specific marker antibody for luminescence labeling, confining liquid, a washing solution, a NaOH water solution, hydrogen peroxideand a calibrator, and can realize simple, rapid and quantitative detection of the specific exosomes; the specific exosome luminescence immune quantitative detection kit can be directly used for detecting a marker of the specific exosomes in blood serum, blood plasma, pleuroperitoneal fluid, urine and cerebrospinal fluid samples; the specific exosome luminescence immune quantitative detection kithas the advantages of high sensitivity, good stability, short detection time and simplicity in operation and is very applicable to clinical detection.

The invention relates to a fluorescence probe, a preparation method and an application thereof. The fluorescence probe is arranged as an addition activating type probe, can be used for specific fluorescent mark of protein and also can be used for ration, detection, dynamic and activity study of protein and cell, tissue and in vivo images.

The invention discloses a zearalenone-vomitoxin double-channel immune quantitative test strip, and belongs to the technical field of immunoassay and rapid detection. A fluorescent probe is prepared bymarking fluorescent microspheres; a fluorescent microsphere-zearalenone monoclonal antibody, a fluorescent microsphere-vomitoxin monoclonal antibody and a fluorescent microsphere-goat anti-rabbit secondary antibody are included, and zearalenone artificial antigen, fluorescent microsphere artificial antigen and goat anti-mouse secondary antibody are sprayed on a nitrocellulose membrane to serve asa detection line T1, a detection line T2 and a quality control line C respectively to prepare the immunochromatographic test strip; and zearalenone and vomitoxin in the sample are analzyed simultaneously and quantitatively by reading the fluorescence value of the detection lines in a fluorescence immunoassay analyzer by means of a competitive immunoassay method. The method overcomes the defect that colloidal gold is not easy to store in the test strip technology, and the method for preparing the fluorescent probe is simple, efficient and high in sensitivity.

The invention relates to the technical field of a biosensor, and particularly relates to a fluorescence biosensor for detecting UDG (Uracil-DNA Glycosylase) on the basis of a polymerase-assisted feedback rolling circle amplification and endonuclease amplification fluorescence method. The invention aims to solve problems of both low specificity and low sensitivity of a method for detecting UDG in the prior art. The biosensor for detecting the UDG on the basis of a feedback rolling circle amplification technology achieves a rolling circle amplification effect on matching of phi29 polymerase andendonuclease IV, implements fluorescence resonance energy transfer of a fluorophore and a Quenching group and performs a homogeneous reaction on mixed liquid. A preparation method comprises: constructing a circular template and a composite probe; feeding back a rolling circle amplification signal and carrying out fluorescence detection. Specific hydrolysis of the UDG on a basic group U is utilized, and by utilizing such specific reaction, the UDG can be accurately measured and meanwhile, interference can also be avoided; by utilizing endonuclease IV circle amplification, a signal amplificationeffect is achieved.

The invention discloses a paper chip enzyme-linked immunoassay test card for a combined multi-tumor marker test. The test card is characterized in that upper, middle and lower filter paper chips, a double-sided tape and a paper-based slide frame are arranged between an upper shell and a lower shell; the upper, middle and lower filter paper chips refer to wax patterned filter paper chips which are provided with hydrophilic and hydrophobic regions and are alternately arranged respectively; a sample introduction and liquid feeding window is formed in an upper shell body; the upper filter paper chip is arranged between the upper shell and the double-sided tape; the middle filter paper chip is arranged between the double-sided tape and the lower filter paper chip, and sensitive regions which are distributed in arrays and used for fixing different reaction reagents are arranged on the middle filter paper chip; the water-based slide frame is arranged between the lower filter paper chip and the lower shell; the upper and middle filter paper chips are bonded through the double-sided tape; the lower filter paper chip slides on the water-based slide frame relative to the upper and middle filter paper chips, so that the different reaction reagents can sequentially flow among the multiple layers of filter paper chips. According to the test card, an early diagnosis device applied in clinical site is provided for patients.

The invention discloses a three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip and a preparation method and application thereof. The preparation method of the chip comprises the following steps of designing the shape of a reaction tank and patterns of electrodes, and then obtaining a screen of the reaction tank and a screen of the electrodes, wherein the electrodes are three-carbon electrodes and include the working electrode, the counter electrode and the reference electrode; conducting screen printing of the shape of the reaction tank on a fabric, conducting screen printing of the patterns of the electrodes on the same fabric, and conducting airing to obtain the three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip. The electrode screen printing processing method is applied onto the base fabric micro-fluidic chip for the first time, the processing method has the advantage that no expensive or complex instruments are needed, and has the other important advantage that the base fabric micro-fluidic chips with the reaction tanks and the electrodes can be obtained in batches. The electrodes are the three-carbon electrodes, and compared with a traditional three-electrode system, the manufacturing cost is low; usage for one time can be achieved, and no complex polishing pretreatment is needed.

The invention discloses a method for detecting exosome small molecule metabolites by using mass spectrometry. The method comprises the following steps of preparing an instrument and a reagent; preparing an iron-containing oxide nanometer particle substrate; preparing an exosome sample; respectively taking the exosome samples diluted according to different proportions; performing sample application onto a target plate; performing drying at the room temperature; dispensing the iron-containing oxide nanometer particle substrate onto the exosome sample; performing drying at the room temperature; performing mass spectrometric detection; analyzing the mass spectrometric detection result to draw the conclusion. The method has the advantages that the micro-nano particle materials are used as the substrate; the qualitative and quantitative detection on molecules to be detected is fast and efficiently realized by using few biological samples in mass spectrometric analysis; meanwhile, the background interference and hotspot effects of the conventional substrate can be avoided; the detection on metabolism molecules in the exosome samples is realized.

The invention discloses a method of detecting biological molecules by a nano enzyme immune sandwich novel technology. The method comprises the following steps: (1) coupling a first monoclonal antibodyto first magnetic nano enzyme particles to obtain a capture probe; (2) coupling a second monoclonal antibody to second magnetic nano enzyme particles to obtain a detection probe; (3) incubating a to-be-detected sample and the capture probe; (4) obtaining a capture probe-antigen compound from a solution in the step (3); (5) resuspending the capture probe-antigen compound, adding the detection probe, and carrying out incubation; (6) obtaining a capture probe-antigen-detection probe compound from the solution in the step (5); (7) adding a peroxide and a catalytic primer to develop color, and detecting the light absorbance value of the solution through an enzyme-linked instrument; and (8) measuring biological molecules of known concentration and drawing a standard curve to obtain the antigencontent in the to-be-detected biological sample. The method is simple and rapid in operating step, environment-friendly and suitable for detecting various chemical or biological molecules.

The invention provides a rapid detection method for the content of aflatoxin in brown rice based on FT-NIR technology. The method comprises the following steps: step 1, sample preparation: a step of collecting brown rice sample with different Aspergillus flavus invasion degrees, crushing the brown rice samples to obtain sample powders and refrigerating the sample powders for detection; step 2, spectral detection: a step of collecting diffuse reflection spectral information of the sample powders by using a Fourier transform near-infrared spectrometer and determining the contents of aflatoxin B1, B2, G1 and G2 and a total amount thereof in the sample powders by using a multifunctional column purification-high performance liquid chromatography-fluorescence method; step 3, spectral pretreatment: a step of pretreating original spectral information of the sample powders obtained in the previous steps so as to eliminate interference; step 4, quantitative forecasting and analysis: a step of analyzing the pretreated spectral information by using a stepwise multivariate linear regression method (SMLR) and establishing a stoichiometrical model of the contents of aflatoxin B1, B2, G1 and G2 and the total amount thereof in the sample powders and the spectral information of the samples; and step 5, rapid determination: a step of outputting the contents of aflatoxin B1, B2, G1 and G2 and the total amount thereof by using the established model on the basis of the spectral information of to-be-detected brown rice.

The invention discloses a quick detector for quality and safety of tea. The detector is provided with a machine shell of which the upper surface is provided with a color comparison cuvette cover. Thedetector is characterized in that: a transmission type color comparison cuvette and a reflection type color comparison cuvette are arranged side by side under the color comparison cuvette cover of themachine shell; the transmission type color comparison cuvette is a rectangular tank body, one side surface of the tank body is provided with a light source/monochromator, and the other opposite sidesurface is provided with a photoelectric detector; and the reflection type color comparison cuvette is a cuboid of which the inside is provided with three T-shaped channel holes, channel openings of two side channel holes are provided with a light source/monochromator respectively, the lower part channel opening of the middle channel hole is provided with a photoelectric detector, and the lower part channel opening of the middle channel hole is provided with a chromatographic plate. The detector can be used for quickly and quantitatively detecting the contents of heavy metal and pesticide in tea in field and quickly detect the quality safety of tea in field.

The invention discloses an HBV detection method. The method comprises the following steps: separating a nucleic acid extract liquid from serum to obtain a sample to be detected; detecting a reaction result by a fluorescent quantitative PCR instrument, and setting a fluorescence signal as fluorescein corresponding to the 5' end fluorescence group of a Taqman fluorescent probe PgRNA-FP when collected, and collecting the fluorescence signal at 60 DEG C; and carrying out result analysis. Specific reverse transcription is carried out according to HBV PgRNA poly A, and then amplification is carried out according to HBV PgRNA specific primers and the probe, so possible interference caused by HBV DNA is effectively eliminated, and the HBV PgRNA detection specificity is effectively improved.

A device for the controlled filling of containers with powdered substance includes a supply vessel with a narrow exit opening for temporarily holding the powdered substance and for discharging a predetermined quantity of the powdered substance into a container located underneath the supply vessel, a vibrator means is connected to the supply vessel for effecting the discharge of the powdered substance. A sensor for determining the quantity of powdered substance discharged from the supply vessel into the container, and a control unit, which converts the data acquired by the sensor into control commands for the vibrator means is included. The sensor includes a capacitive sensor for the quantitative determination of the quantity of powdered substance falling there through during the filling process and is located between the supply vessel and the container.

The invention discloses a retinol conjugated protein detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, the reagent R2 is the mixture of retinol conjugated protein antibody sensitized polystyrene latex particles and the buffer solution. The retinol conjugated protein detection kit has the advantages that the kit is simple and quick to detect, high in sensitivity, good in accuracy, good in anti-interference capacity and low in production cost; a lipoprotein a (retinol conjugated protein) detection method adopted by the kit is a latex enhanced immuno-turbidimetry and enables the lipoprotein a (retinol conjugated protein) detection to be more economical, convenient and quicker, and the retinol conjugated protein detection kit is applicable to automatic biochemical analyzers of most of hospitals and especially can achieve quick quantitative detection for emergency treatment.