21results about How to "Rapid Quantitative Detection" patented technology

The invention relates to the technical field of molecular biological detection, and discloses a composition for detecting pathogenic microorganisms, a kit and application of the composition and the kit. The composition provided by the invention can simultaneously comprise specific primers aiming at a plurality of different pathogenic microorganisms and has excellent specificity and detection sensitivity to the specific pathogenic microorganisms, and different primers do not generate dimerization, so that the different pathogenic microorganisms can be simultaneously, rapidly and accurately detected.

The invention belongs to the technical field of biochemical sensing, and particularly relates to a sulfide colloidal quantum dot-based biosensor for exosome detection as well as preparation and application thereof. Transferring the sulfide colloidal quantum dot solution to a metal gate electrode of a high electron mobility transistor or a working electrode of a screen printing three-electrode to form a sulfide colloidal quantum dot film; coating the sulfide colloidal quantum dot film with an antibody protein modification layer, and closing non-specific active sites on the antibody protein modification layer by adopting bovine serum albumin; the antibody protein can be specifically bound with transmembrane protein corresponding to the surface of an exosome to be detected, so that the exosome to be detected is captured on the surface of the electrode, and meanwhile, based on the unique physical and chemical effect of the sulfide colloidal quantum dots, the modified electrode has the capability of sensing the distribution change of external charges, so that the detection sensitivity of the exosome to be detected is improved. Therefore, rapid and quantitative detection of the exosome is realized.

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.

The present invention proposes a method for quantitatively detecting the hydroxyl value of terminal hydroxyl polybutadiene compounds, and belongs to the technical field of detection of high molecular polymers. The method comprises the following steps: using a mixed monolayer loading method or a three-layer loading method, HTPB sample solution, DCTB matrix solution and silver trifluoroacetate solution are applied to a sample target, dried naturally, and co-crystallized between the sample solution and the matrix solution to form a thin layer, and the thin layer is subjected to MALDI-TOF MS analysis to obtain a sample ion peak, and the sample ion peak is calibrated, and the amount of the substance of the terminal hydroxyl polybutadiene is calculated according to the number average molecular weight of the terminal hydroxyl polybutadiene, and the content of the terminal hydroxyl group is calculated according to the ratio of different hydroxyl end structures. The method for quantitatively detecting the terminal hydroxyl value of a polymer of the present invention has simple sample preparation, high accuracy, no strict requirements on the purity of the sample, rapid data acquisition, simple processing, and is more suitable for rapid quantitative detection.

The invention discloses a method for detecting various heavy metal ions based on Raman spectrum. The method comprises the following steps: reducing and dispersing gold nanoparticles by using quaternized xylan, coating the surface of paper with the gold nanoparticles, and then reducing silver nanoparticles on the surface of the paper in situ; carrying out ion exchange by using a solution containing anions, and adding Raman beacon molecules to prepare detection test paper; preparing a standard heavy metal cation solution, dripping the standard heavy metal cation solution on the surfaces of different detection test paper, reacting the exchanged anions with the specific heavy metal cation standard solution to generate precipitates, proportionally influencing the Raman signal intensity, collecting Raman signals, and establishing a Raman intensity standard curve of various heavy metal ions; and finally, dripping the to-be-detected solution containing the heavy metal cations on the surface of the detection test paper, carrying out Raman spectrum detection on the detection test paper dripped with the to-be-detected solution, and calculating to finish the detection. The method can realize quantitative detection of heavy metal cations in a to-be-detected solution, and has the advantages of low detection limit and high sensitivity.

This invention belongs to the field of fetoglobulin B detection technology, and discloses an immunochromatographic test strip for quantitative detection of fetoglobulin B using quantum dot fluorescent microspheres and its preparation method. The immunochromatographic test strip of this invention includes a sample pad, a release pad, and a nitrocellulose membrane; the release pad is coated with a fetoglobulin B monoclonal antibody labeled with quantum dot fluorescent microspheres and a mouse IgG antibody labeled with quantum dot fluorescent microspheres; the nitrocellulose membrane has a detection line and a control line, the detection line is coated with fetoglobulin B monoclonal antibody, and the control line is coated with goat anti-mouse IgG antibody. The immunochromatographic test strip of this invention reduces the detection time to 9-12 minutes, and the total detection time is only 20 minutes, achieving rapid and accurate quantitative detection of fetoglobulin B. It also features high detection sensitivity, a wide detection range, and simple and rapid operation, which is beneficial for the screening and monitoring of atherosclerotic coronary heart disease.

The present application belongs to the field of health quarantine, and relates to a borrelia burgdorferi rapid detection kit and a detection method. The kit can perform on-site rapid quantitative detection on a sample to be detected based on fluorescence microsphere immunochromatography technology. The kit comprises a borrelia burgdorferi fluorescence microsphere immunochromatography test strip. The test strip comprises a viscous bottom liner and viscous bottom plate, and is sequentially pasted with an analysis membrane, a binding pad, a water absorption pad and a sample pad. The analysis membrane is a nitrocellulose membrane, which has a detection band and a quality control band, and is respectively fixed with borrelia burgdorferi antigen protein and rabbit anti-SPA. The binding pad is fixed with an SPA and fluorescence microsphere labeled compound. The present application can realize on-site rapid quantitative detection of borrelia burgdorferi carried by mice, and has the advantages of simple operation, short detection time, high detection sensitivity, good specificity, convenient storage and easy popularization and use.

The invention relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disruption activity in sewage and application. A Y-type polypeptide containing an anchoring region, a hinge region and an antifouling region is creatively designed as a structural skeleton, and the tail ends of a main chain and a branch chain are respectively subjected to directional modification to prepare the multifunctional ligand-polypeptide-dye conjugate; an optical biosensing chip, namely a ligand-polypeptide-dye conjugate functionalized biosensing chip which is high in reaction efficiency, strong in anti-pollution characteristic and capable of being stably regenerated, is constructed by adopting a covalent coupling method, and then the quantum dot-nuclear receptor fluorescent probe which is high in sensitivity and adjustable in emission spectrum is prepared. The ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is provided, rapid quantitative detection of the endocrine disruptors in sewage is achieved, and the endocrine disruptors have broad-spectrum specific recognition capacity on a certain type of endocrine disruptors.

The present invention provides a biosensor and a kit for detecting algae toxins, including an aptamer Apt, a T chain, a waste chain, a primer1 chain, a primer2 chain, an auxiliary chain H, a rolling circle template chain padlock and a hairpin HP, and the nucleotide sequence thereof is shown in SEQ ID NO: 1-8. The sensor of the present invention has good analytical performance, and has the advantages of being visible to the naked eye, fast detection speed, simple operation, low price, low detection limit, and high specificity. It can make up for the defects and shortcomings of the existing detection methods of microcystin, and realize rapid and accurate quantitative detection thereof. The kit prepared by this research has methodological versatility for the detection of microcystin, and has potential application prospects in food samples and environmental monitoring.

The invention belongs to the technical field of heavy metal ion detection, and particularly discloses a preparation method of an ionic liquid modified nanogold cluster material and application of the ionic liquid modified nanogold cluster material in detection of heavy metal ion cadmium. According to the invention, bovine serum albumin is taken as a stabilizing agent and a reducing agent, a nanogold cluster emitting red fluorescence is synthesized, and the ionic liquid modified nanogold cluster composite material is synthesized by utilizing electrostatic interaction between imidazolium ionic liquid and BSA (Bovine Serum Albumin). Due to the unique coordination effect between the cadmium ions and the imidazolium ionic liquid, the cadmium ions can effectively enhance the fluorescence intensity of the ionic liquid modified nanogold cluster composite material, the ionic liquid modified nanogold cluster composite material can be applied to detection of the cadmium ions in an actual sample, and a new thought is provided for convenient detection of the cadmium ions in a complex matrix.

The present invention belongs to the field of biological detection technology, and specifically relates to a fluorescent probe and its preparation method and application, and a method for detecting the content of fumonisin. The fluorescent probe provided by the present invention has an acyl chloride structure, can quickly complete a derivatization reaction with fumonisin, and the amide structure generated by the derivatization reaction can form a derivative of a larger conjugated system with the parent of the fluorescent probe. The derivative is more likely to generate a high fluorescent signal under ultraviolet light excitation, thereby improving the fluorescence efficiency of the fluorescent probe, and the detection limit reaches 0.12μg / L.

The present invention discloses a novel multifunctional MOF on MOF gel material and its preparation method and application, named FCZE, the gel material is a core-shell structure, with a bimetallic organic framework MOF as the core, a Uio-66 / Eu layer as the shell, the bimetallic is Fe and Cu, Uio-66 and Eu are coordinated and connected in the Uio-66 / Eu layer, and the bimetallic organic framework MOF is first prepared using copper salt, iron salt and trimesic acid; then pyromellitic acid, zirconium salt and europium salt are added to prepare the MOF on MOF gel material. The MOF on MOF gel material prepared by the present invention integrates different types of signal conversion mechanisms (fluorescence and colorimetry) through a unique composite structure design, and realizes rapid and sensitive detection of CTC, CIP and D-PA at the same time.

The invention relates to the field of nanopores, in particular to a nanopore sequencing-based small molecule compound detection method, which comprises the following steps: S1, obtaining a first incubation product; s2, obtaining a second incubation product; s3, obtaining a third incubation product; s4, obtaining a first purified product; s5, obtaining a first long-chain nucleic acid and a second long-chain nucleic acid; s6, obtaining a PCR amplification product; s7, carrying out nanopore sequencing; and S8, analyzing the existence and / or content of the small molecule compound in the biological sample. The method can rapidly detect the small-molecule compounds, can rapidly detect mixed samples of various small-molecule compounds, and is high in specificity, low in detection limit and suitable for various complex biological samples.

The invention discloses an enzyme-linked immunosorbent assay kit and method for detecting capsaicin in animal and vegetable oils, and belongs to the technical field of immunoassay. The enzyme-linked immunosorbent assay kit comprises a standard diluent, a sample diluent, an antibody / enzyme conjugate diluent, a capsaicin standard, an enzyme-labeled plate coated with a capsaicin antigen, a capsaicin antibody, an enzyme conjugate, a washing solution, a color developing solution, and a stop solution. The enzyme-linked immunosorbent assay kit provided by the invention has wide applicability, can stably, rapidly, and efficiently perform quantitative detection of capsaicinoids in a variety of animal and vegetable oils, and has the advantages of high sensitivity, low detection limit, etc. The sensitivity of the kit is 0.1 μg / kg, the detection limit is 0.3 μg / kg, and the quantitative range is 0.4-10 μg / kg. Therefore, it has good application prospects in the rapid detection of vegetable crude oil and kitchen recycling oil.

The invention discloses a kit for detecting picoxystrobin, a preparation method and application thereof. The picoxystrobin hapten prepared by the invention retains the characteristic structure of picoxystrobin to the greatest extent, significantly enhances the immunogenicity of the picoxystrobin hapten, and the obtained picoxystrobin monoclonal antibody has high sensitivity. Through ELISA detection technology, the minimum detection limit of picoxystrobin is 0.5 μg / L. The preparation method of the picoxystrobin hapten has simple reaction operation, easy control of reaction conditions, and high purity and yield of the prepared picoxystrobin hapten. The picoxystrobin fluorescent quantitative immunochromatographic test strip prepared by the invention has a detection sensitivity of up to 1 μg / L, high precision, can be stably stored at room temperature for more than one year, is conducive to storage and transportation, and can realize rapid quantitative detection of picoxystrobin.

A biomarker detection system based on gas generation comprises a closed container, a capillary tube and a scale mark, a magnetic bead-nucleic acid aptamer compound is used for specific recognition and capture of target protein, a platinum nanoparticle-nucleic acid aptamer compound catalyzes hydrogen peroxide to generate oxygen, a gas pressure difference is formed to push a liquid column to move, and the target protein is detected through the scale mark. The concentration of the target protein is measured by reading the length of a liquid column in a capillary tube with naked eyes, and quantitative detection is realized. The detection system improves the detection sensitivity and speed, reduces the operation complexity and cost, has high expansibility, can detect various biomarkers, and is suitable for diagnosis of various diseases such as tumors and the like. The system has the advantages of high sensitivity, rapidness, low cost and wide application prospect.

The invention belongs to the technical field of bacterium detection, and particularly relates to an SERS probe and a preparation method and application thereof.The preparation method of the SERS probe comprises the steps that hydroxylamine, sodium hydroxide, silver nitrate, tetrachloroauric acid and trisodium citrate are mixed and react to obtain the SERS probe Au / Ag FL; in addition, the SERS probe Au / Ag FL (at) I-can be obtained through KI modification. The SERS probe disclosed by the invention is used for detecting pathogenic bacteria, and the sensitivity and the accuracy are high.

This invention discloses a method for preparing a plasmonic-enhanced fluorescence-coupled aptamer ratio sensor based on gold nanoparticles and its application in the visual detection of amyloid protein. The invention involves preparing gold nanoparticles and immobilizing them on an amino-modified ELISA plate by adding EDC / NHS. β-amyloid aptamers, with amino and thiol groups modified at both ends respectively, are grafted onto the gold nanoparticle-modified ELISA plate via Au-S bonds. EDC / NHS is then added to graft Rosa Bengal onto the aptamers on the gold nanoparticles via amide bonds, resulting in a plasmonic-enhanced fluorescence-coupled aptamer as the responsive fluorescent probe and 9-anthracarboxylic acid as the reference fluorescence, thus creating a plasmonic-enhanced / ratio-fluorescent probe sensor. The plasmonic-enhanced / ratio-fluorescent probe sensor prepared by this invention exhibits good stability, enabling portable, ultrasensitive, and specific visual quantitative detection of β-amyloid protein.

This invention discloses a method for synthesizing a standard sample for detecting tartrazine intermediates as shown in structural formula 1, characterized by the following reaction formula 1. This patent provides a method for preparing a standard sample for detecting the content of tartrazine intermediates, the structure of which is shown in structural formula 1, enabling rapid and accurate quantitative detection of 1-(4'-sulfonylphenyl)-3-carboxylic acid methyl ester-5-pyrazolone monosodium salt in tartrazine.

The invention discloses a molecular beacon probe composition, a kit and application thereof, and relates to the technical field of molecular biology. A molecular beacon probe is introduced into an in-vitro transcription system, and the probe emits a fluorescence signal through specific binding with an in-vitro transcription product-RNA target sequence. In a real-time fluorescence monitoring system, the intensity of a probe fluorescence signal is in direct proportion to the RNA concentration transcribed in vitro, so that real-time quantitative detection of an in-vitro transcription product can be realized. In addition, specific probes are respectively arranged on the in-vitro transcription product RNA, and the yield of the in-vitro transcription full-length RNA can be obtained by comparing the fluorescence difference of the two probes. The sequence of the molecular beacon probe can be in specific complementary pairing with partial sequence of in-vitro transcription RNA, so that a target transcription product can be accurately recognized, non-specific binding is avoided, the reliability of data is ensured, and high-sensitivity quantitative analysis is provided.

The present application relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disrupting activity in sewage and application. The present application creatively designs a Y-shaped polypeptide containing an anchoring region, a hinge region and an anti-fouling region as a structural framework, and performs directional modification at the ends of the main chain and the branch chain to prepare a multifunctional ligand-polypeptide-dye conjugate; a covalent coupling method is used to construct a high-response-efficiency, strong-anti-pollution-characteristic and stable-regeneration optical biosensor chip, i.e. a ligand-polypeptide-dye conjugate functionalized biosensor chip, then a quantum dot-nuclear receptor fluorescence probe with high sensitivity and adjustable emission spectrum is prepared, a ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is proposed, and rapid quantitative detection of endocrine disruptors in sewage is realized, so that the present application has broad-spectrum specific recognition ability for certain endocrine disruptors.