Method for detecting hoptoglobin in serum and detecting kit thereof

A technology of haptoglobin and reagents, applied in the field of immunological detection, can solve the problems of slow display results, failure to widely carry out, large errors, etc., and achieve the effect of simple and easy detection method
CN102128935AInactive Publication Date: 2011-07-20BIOSINO BIO TECH & SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BIOSINO BIO TECH & SCI
Publication Date
2011-07-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for simply and quickly detecting hoptoglobin in serum, namely an immunoturbidimetry, and a detecting kit thereof. The method is a detecting method designed by using the principle that the hoptoglobin is a human protein, has antigenicity and generates cohesion after combining with an antigen antibody. The hoptoglobin in the serum sample is combined with adequate specific antibody in the agent to form an insoluble immune complex; the change of the absorbance of the reaction solution is relevant to the content of the hoptoglobin in the serum sample. The method for detecting the hoptoglobin in the serum and the detecting kit in the invention have the advantages that: the serum sample is not subjected with special treatment, and can be ordinarily detected on an automatic biochemistry analyzer; the result directly reflects the true protein content of the hoptoglobin in the serum sample; therefore, the invention is used for clinic detection.
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Description

technical field

[0001] The invention relates to an immunological detection method, in particular to an immunological detection method for haptoglobin in serum and a detection kit thereof. Background technique

[0002] Haptoglobin (HP), also known as haptoglobin, is widely present in the blood and other body fluids of humans and many mammals, and its content in individuals is relatively stable. Its biosynthesis and degradation are mainly carried out in the liver. Haptoglobin has the ability to bind to free hemoglobin, and acts like a transporter of hemoglobin. A molecule of haptoglobin can bind to a molecule of free hemoglobin and form a complex, which is quickly absorbed by mononuclear-macrophages in the blood. Cells are disposed of. This process prevents the filtration of hemoglobin from the glomerulus and avoids damage to the kidneys caused by free hemoglobin. During this metabolic process, haptoglobin is also degraded, manifested as a decrease in the level of haptoglobi...

Claims

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