119 results about "Pharmacological Substance" patented technology

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine prescription efficacy quantification method and system.The method comprises the steps that chemical component spectrums, pharmacodynamic substance contents and biological activity indexes of traditional Chinese medicines forming a prescription are obtained; constructing a multi-target regulation and control network by combining a traditional Chinese medicine-target interaction database according to the chemical component spectrum, the pharmacodynamic substance content and the biological activity index, identifying a core efficacy pathway cluster by adopting a Louvain algorithm, and calculating a pathway activation intensity value by adopting a pathway target membership weighting model; according to the core efficacy pathway cluster, establishing a mapping relation between the core efficacy pathway cluster and traditional Chinese medicine efficacy terms through ontology and semantic mapping, and according to pathway activation intensity values, generating a prescription efficacy intensity vector through a weighted aggregation model; according to the efficacy intensity vector of the prescription, verifying and correcting the network weight through an in-vitro cell model, and generating the efficacy quantification of the prescription in combination with the clinical syndrome calibration coefficient. Therefore, the problems of single data dimension and insufficient quantification result accuracy in the prior art are solved.

The invention discloses a construction method of a gout compound decoction fingerprint as well as the fingerprint and application thereof, and belongs to the technical field of analysis and detection. The construction method of the fingerprint spectrum comprises the following steps: taking a test solution prepared from the gout compound decoction; the test solution is subjected to HPLC-SPD detection, so that the fingerprint spectrum of the gout compound decoction is obtained, and the HPLC detection conditions are that a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, and a C18 chromatographic column is used for detecting the fingerprint spectrum of the gout compound decoction. An aqueous solution containing 0.1%-0.2% of phosphoric acid is used as a mobile phase A, and acetonitrile is used as a mobile phase B for gradient elution; the flow velocity is 0.8-1 mL / min, the column temperature is 25 DEG C, the sample injection volume is 5-10 [mu] L, and a diode array detector is 190-400 nm full-wave band scanning. The chemical components in the gout compound decoction are comprehensively and systematically analyzed, and a basis is provided for quality control and in-depth study of a pharmacodynamic material basis.

The invention provides a large and small model collaborative traditional Chinese medicine prescription optimization method and system, a terminal and a storage medium, and the method comprises the steps: firstly determining a prescription needing to be optimized, sorting related public literatures into an input document, constructing a local prescription knowledge base, and carrying out question answering based on a prescription optimization large language model to obtain a large model optimization prescription; then, determining related pathway targets of applicable symptoms in the prescription needing to be optimized, collecting chemical substances in the prescription needing to be optimized to obtain a prediction result of a pharmacodynamic substance level, performing medicinal material tracing analysis on the prediction result, optimizing prescription composition based on pharmacodynamic substance weight to obtain a small model optimized prescription, and determining the pharmacodynamic substance weight based on the small model optimized prescription. And performing comprehensive analysis based on the large model optimization prescription and the small model optimization prescription to generate final prescription optimization recommendation. The efficient traditional Chinese medicine prescription composition optimization method is constructed from the macroscopic level and the microscopic level, and the scientificity and the practicability of prescription optimization can be improved.

The present invention discloses a γ-arylbutenolide compound, a preparation method thereof, and an application thereof in the preparation of cardiovascular protective drugs. The above-mentioned structurally novel γ-arylbutenolide compound is isolated for the first time from the fermentation culture of the marine herbal soft coral symbiotic fungus Aspergillus sp. EGF7-0-1, and it is found for the first time that the above-mentioned compound has a protective effect on the cardiovascular system, providing a pharmacodynamic substance basis for the subsequent research on cardiovascular diseases.

The present invention relates to the use of TDP1 and eltrombopag in the treatment of myotonic dystrophy type 2. The present invention uses a fruit fly model to identify that TDP1 knockdown can effectively rescue neurodegeneration in the CCTG repeat fruit fly model, reduce pigment production block, cell death and ommatidium fusion, and improve motor defects. At the same time, in vitro screening using small molecules found that eltrombopag can be used as a TDP1 inhibitor and can significantly reduce disease cytotoxicity. The present invention provides a new target and pharmacological substance for CCTG repeat expansion diseases in myotonic dystrophy type 2, and expands the medical use of eltrombopag. The present invention provides a new drug with clinical application prospects for the treatment of CCTG repeat expansion diseases in myotonic dystrophy type 2.

The invention discloses a method for detecting anions in a phlegm-heat-clearing injection, the detection method comprises the following steps: a test solution and a reference solution are taken for detection, the phlegm-heat-clearing injection is composed of scutellaria baicalensis, bear gall powder, cornu gorais, honeysuckle, fructus forsythiae and propylene glycol; chromatographic conditions of the detection are as follows: an anion exchange chromatographic column is adopted, an eluent is an acid aqueous solution, an alkali aqueous solution and / or a buffer salt aqueous solution, the flow velocity is 0.1-2.0 ml / min, the column temperature is 20-40 DEG C, a detector is an electrical conductivity detector, the detection mode is suppression electrical conductivity detection, and the sample size is 10-40 [mu] l; and obtaining the content information of the anions in the phlegm-heat clearing injection according to the detection result. The method disclosed by the invention is simple, convenient, efficient and rapid, and provides a theoretical basis for the quality control of the phlegm-heat clearing injection and the deep research of a pharmacodynamic material basis by detecting the content of the anions.

The invention discloses a substance basis and a detection method for the drug effect of liver-bean jelly, and belongs to the technical field of medicines. In-vitro chemical components of liver-bean jelly are combined to track blood entering components of liver-bean jelly and carry out attribution analysis; obtaining an action target spot of the liver-bean linging for intervening the WD hepatic fibrosis; screening metabolic markers for significant callback of liver-bean linging, and performing enrichment analysis to obtain a significant metabolic pathway; constructing a'small molecule metabolite-reaction-enzyme-target 'composite metabolic network by the action target and the significant callback metabolic marker, and screening a core action target, a core metabolic marker and a core metabolic pathway; a molecular docking technology is used for screening a liver-bean drug effect material basis, and a multi-dimensional network of the liver-bean drug effect material basis, a core action target, a core metabolic marker, a core metabolic pathway and WD liver fibrosis is visualized. The invention discloses a pharmacodynamic material basis and a metabolic regulation mechanism for treating WD hepatic fibrosis by using liver bean paste.

The present application discloses a method for constructing a fingerprint of a gout compound decoction and its fingerprint and application, and belongs to the field of analytical detection technology. The method for constructing the fingerprint comprises the following steps: taking a test solution prepared from a gout compound decoction; performing HPLC-SPD detection on the test solution to obtain a fingerprint of the gout compound decoction, wherein the high performance liquid chromatography detection conditions are: using a C18 chromatographic column; using an aqueous solution containing 0.1% to 0.2% phosphoric acid as mobile phase A, and acetonitrile as mobile phase B for gradient elution; the flow rate is 0.8 to 1 mL / min, the column temperature is 25 ° C, the injection volume is 5 to 10 μL, and the diode array detector is scanned at 190-400 nm full band. The present application conducts a comprehensive and systematic analysis of the chemical components in the gout compound decoction, providing a basis for in-depth research on quality control and the basis of pharmacological substances.

The invention relates to a purification and application method of a self-assembled supramolecular substance based on a radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root decoction, which comprises the following steps: proportioning radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root, adding water, decocting to obtain a GQD decoction, sequentially carrying out ultrafiltration and alcohol precipitation treatment on the supernatant of the GQD decoction, volatilizing the obtained supernatant, redissolving, and freeze-drying to obtain the self-assembled supramolecular substance, and purifying to obtain the self-assembled supramolecular substance based on the self-assembled supramolecular substance based on the radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root decoction. The compound is further used for preparing anti-inflammatory and anti-UC drugs; according to the invention, the anti-UC characteristic of the traditional Chinese medicine composition is utilized to recover intestinal flora with UC disorder and repair damaged intestinal barriers, meanwhile, core pharmacodynamic substances are reserved to the greatest extent, other irrelevant or harmful impurities can be reduced, and the traditional Chinese medicine composition is suitable for separating and purifying a nano-scale supramolecular compound with biological activity in a traditional Chinese medicine decoction to improve the curative effect and reduce toxic and side effects, so that the traditional Chinese medicine composition is suitable for clinical application. And the intestinal micro-ecology and mucous membrane barrier function can be repaired.

The invention provides rhizoma alismatis purified polysaccharide as well as a preparation method, an identification method and application thereof, the rhizoma alismatis purified polysaccharide is a functional component with a novel structure in a traditional Chinese medicine rhizoma alismatis water extract obtained by further extraction and purification, the administration efficiency is effectively improved, and the pharmacodynamic substances are clarified; in-vivo and in-vitro experiments show that the polysaccharide can improve the antioxidant capacity of the liver and relieve inflammatory response; synthesis and metabolism of liver bile acid can be adjusted, cholestasis of the liver is relieved, and bile acid circulation is promoted so as to relieve acute liver injury caused by acetaminophen; the effect of promoting the recovery of liver injury patients is obvious; and the compound also can be used as prebiotics to improve the homeostasis of intestinal microorganisms and promote the synthesis of short-chain fatty acids so as to have beneficial effects on the liver.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

The application relates to the technical field of pharmaceutical analysis, and discloses a pharmacokinetic analysis method of Tongwei Xiaozheng medicinal liquid, which adopts Q-Orbitrap high-resolution liquid chromatography-mass spectrometry to analyze the concentration of Jasmonic acid in blood components of the Tongwei Xiaozheng medicinal liquid in rat plasma, establishes a plasma concentration determination method for the blood original component Jasmonic acid, carries out methodological verification such as specificity, precision and accuracy, and then calculates pharmacokinetic parameters. In the Tongwei Xiaozheng medicinal liquid, 92 blood components are identified, 17 of which are original components and 75 of which are metabolites, the application firstly determines that Jasmonic acid is one of the blood original components of the Tongwei Xiaozheng medicinal liquid, and reveals the pharmacokinetic characteristics of the Jasmonic acid in normal and model rats. The method has strong specificity, high sensitivity and good reproducibility, and can provide a scientific basis for the research on the pharmacodynamic material basis of the Tongwei Xiaozheng medicinal liquid, the establishment of quality control standards and the clinical rational use of drugs.

The invention provides an ultra-high performance liquid chromatography-quadrupole rod / electrostatic field orbitrap high-resolution mass spectrometry detection method for a four-season antiviral mixture, which adopts ultra-high performance liquid chromatography-quadrupole rod / electrostatic field orbitrap high-resolution mass spectrometry, and the optimal chromatographic conditions are as follows: 0-1.0 min, 98%-98% A and 2.0%-2.0% B; 1.0-14.0 min, 98%-70% of A and 2.0%-30.0% of B are added; in 14.0-25.0 min, 70%-0% of A is adopted, and 30%-100% of B is adopted; 25.0-28.0 min, 0%-0% of A and 100%-100% of B are included; from 28.0 min to 28.1 min, 0%-98% of A is adopted, and 100%-2% of B is adopted; 28.1-30.0 min, 98%-98% of A and 2%-2% of B are included; 148 chemical components, including 31 glycosides, 14 terpenoids, 54 flavonoids, 10 lignans, 23 organic acids, 3 alkaloids and 13 other types, are identified in total. The detection method can provide reference for internal quality detection of the traditional Chinese medicine, research on basic components of pharmacodynamic substances of the traditional Chinese medicine and authenticity identification of traditional Chinese medicine components.

The invention belongs to the technical field of medicinal material screening, and relates to a traditional Chinese medicine compound for treating attention deficit hyperactivity disorder and a screening method thereof. The screening method comprises the following steps: constructing a drug use database of ADHD; performing statistics and association rule analysis on frequency, property and flavor tropism and efficacy of the traditional Chinese medicines to obtain a high-frequency traditional Chinese medicine composition; screening ADHD disease targets and establishing a target set; performing clustering analysis and integration on the high-frequency traditional Chinese medicine composition to obtain a core medicine composition; constructing candidate traditional Chinese medicine compounds through reverse network pharmacological analysis; evaluating the efficacy of the candidate traditional Chinese medicine compound through animal experiments, and screening out the optimal traditional Chinese medicine compound. According to the method, an O-R-S analysis mode is constructed through data mining and reverse network pharmacology, traditional Chinese medicine compound screening and evaluation processes of ADHD and candidate compounds with better comprehensive performance are optimized, and a reference scheme with data support and mechanism basis is provided for traditional Chinese medicine clinical treatment and new drug research and development of ADHD.

The present application relates to the technical field of biological medicine, and more particularly to the application of isodon japonicus in the preparation of a drug for preventing and treating spontaneous abortion. The present application analyzes the in-vivo migration components of Jianwei Shoutai Pills, excavates the pharmacodynamic material basis for preventing and treating spontaneous abortion, and uses a CBA / J x DBA / 2 peri-implantation period abortion mouse model to conduct pharmacodynamic experiments. The results show that isodon japonicus can significantly reduce the abortion rate of the CBA / J x DBA / 2 peri-implantation period abortion model mice, and can effectively increase the embryo and placenta wet weight of the CBA / J x DBA / 2 peri-implantation period abortion model mice. That is, isodon japonicus can effectively inhibit spontaneous abortion, has a significant curative effect, has considerable treatment potential, provides a potential new drug selection for clinical drugs for preventing and treating spontaneous abortion, and has a broad application prospect.

The invention discloses a method for analyzing a pharmacodynamic material basis and metabolites of Longqing capsules. According to the method, an UPLC-Q-Exactive-Orbitrap MS technology is adopted, under the same chromatographic condition and mass spectrum condition, the pharmacodynamic material basis and metabolites of the Longqing capsules are measured and analyzed, 128 compounds are analyzed from the Longqing capsules, 144 exogenous compounds including 61 prototype components and 83 metabolites are analyzed from plasma, urine and excrement of rats in total, and the pharmacodynamic material basis and the metabolites of the Longqing capsules are analyzed to form the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules. The main metabolic pathways comprise oxidation, reduction, hydrolysis, methylation, sulfation, glucuronization, composite reactions of various reaction types and the like. The invention provides a scientific basis for quality control and clinical reasonable medication of Longqing capsule medicines.

The application discloses a kind of material evaluation methods, pharmaceutical compositions and application of sophora fruit products.It is specific, disclose a kind of material evaluation methods of sophora fruit products, it includes the following steps: S1, using liquid chromatography, the fingerprint of the sophora fruit product of different processing time is established, and common characteristic peak is extracted;S2, the hemostatic effect of sophora fruit product of different processing time is determined;S3, according to the characteristic peak data of the sophora fruit product of different processing time and its hemostatic effect data, statistical analysis is carried out by chemometrics method, and the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect

The present invention belongs to the technical field of organic chemical synthesis, and specifically relates to a rhein nitroxide derivative, a synthesis method, and an application thereof. The present invention uses rhein, an antioxidant pharmacological substance from the traditional Chinese medicine rhubarb, as a research object, selects its carboxyl group at position 3 as a modification site, and introduces four active intermediate nitroxides into the rhein structure based on the amide condensation and esterification reaction mechanisms and the pharmacophore splicing principle. Thirty rhein nitroxide derivatives were designed and synthesized, thereby establishing a method for synthesizing novel rhein nitroxide derivatives with mild reaction conditions, environmental friendliness, and high yield. The provided novel rhein nitroxide derivatives have the ability to scavenge DPPH and ABTS free radicals, have a protective effect on oxidatively damaged cells, and have the ability to delay the lifespan of Caenorhabditis elegans. Their effects are superior to those of rhein, providing a new approach for the development of antioxidant and anti-aging drugs.

This invention belongs to the field of modern analytical technology of traditional Chinese medicine, specifically involving a method for detecting chemical components of bitter vine based on UPLC-Q-TOF-MS / MS. This invention is the first to systematically and rapidly perform qualitative analysis on the chemical components of bitter vine, a Dai medicinal herb, identifying a total of 96 compounds, including saponins, phenylpropanoids, flavonoids, terpenes, and organic acids. This provides a scientific basis and data support for elucidating the pharmacodynamic material basis, quality control, and subsequent formulation development of bitter vine.

This invention provides a method for screening the active ingredient groups of Xinsu Ning capsules, relating to the technical field of quality control and pharmacodynamic substance analysis of traditional Chinese medicine. The screening method for the active ingredient groups of Xinsu Ning capsules provided by this invention integrates non-targeted chemical component analysis, in vivo prototype and metabolite detection, network pharmacology correlation analysis, and fingerprint spectrum construction, enabling a systematic evaluation of the in vivo exposure characteristics, target regulatory potential, and quality consistency of candidate components. This method can identify chemical components related to pharmacodynamic effects from complex systems, providing an operable technical path for the quality control and pharmacodynamic substance basis research of Xinsu Ning capsules.

The application provides a method for detecting the pharmacodynamic components of a traditional Chinese medicine composition by UPLC-MS / MS. The method can identify 14 pharmacodynamic components of the traditional Chinese medicine composition, including saikosaponin A, saikosaponin D, naringin, paeoniflorin, hesperidin, chicoric acid, chlorogenic acid, salvianolic acid B, zephyranthine, kaempferitrin, and codariusrhizome glycoside, amargin, hyperoside and rutin, thereby providing a reference for evaluating the intrinsic quality of the traditional Chinese medicine composition.

The present invention provides a method for detecting the HPLC fingerprint of Atractylodes macrocephala. The present invention provides an Atractylodes macrocephala medicinal material, which uses the above-mentioned detection method to detect the content of four components: atractylodes lactone I, atractylodes lactone II, atractylodes lactone III, and atractylodes ketone. Each gram of Atractylodes macrocephala medicinal material contains 0.21-0.32 mg of atractylodes lactone I, 0.13-0.25 mg of atractylodes lactone II, 0.1-0.19 mg of atractylodes lactone III, and 5.21-14.08 mg of atractylodes ketone. The present invention also provides an HPLC fingerprint of Atractylodes macrocephala and a method for determining the quality of Atractylodes macrocephala medicinal material. The present invention also provides a method for screening quality-marking components of Atractylodes macrocephala medicinal material. The present invention adopts the HPLC-DAD multi-wavelength switching method to establish the HPLC fingerprint of Atractylodes macrocephala, conducts chemometric research at the same time, and determines the contents of atractylodes lactone I, atractylodes lactone II, atractylodes lactone III and atractylodesone. On the one hand, it can comprehensively evaluate the quality of Atractylodes macrocephala, provide a reference for the quality evaluation and control of Atractylodes macrocephala medicinal materials and decoction pieces, and also lay the foundation for the research on its pharmacological substance basis and related compound research; on the other hand, it can provide a reference for the improvement of the quality standard of Atractylodes macrocephala.

The invention discloses a metabolic regulation and control mechanism analysis method for treating WD hepatic fibrosis by using hepatolianling, and belongs to the technical field of medicines, and the method comprises the following steps: performing pharmacological research on components entering blood and a network; the method comprises the following steps: acquiring a metabolic marker of the remarkable callback of the liver-bean lingering on the basis of serum metabonomics, and performing enrichment analysis on the metabolic marker of the remarkable callback of the liver-bean lingering to obtain a remarkable metabolic pathway; a'small molecule metabolite-reaction-enzyme-target 'composite metabolic network is constructed; screening a liver bean drug effect material basis by a molecular docking technology; and a multi-dimensional network of the Ganguoling pharmacodynamic material basis, the core action target, the core metabolic marker, the core metabolic pathway and the WD hepatic fibrosis is visualized. According to the application, 25 drug effect material bases, 11 core action targets, 12 core metabolic markers and 9 core metabolic pathways are obtained.

The invention discloses a thin-layer identification method for a cornu gorais medicinal material in a phlegm-heat-clearing injection, which comprises the following steps: (1) pre-treating the phlegm-heat-clearing injection to obtain a test solution, and pre-treating a reference medicinal material or a reference substance to obtain a reference medicinal material solution or a reference substance solution; and (2) carrying out thin-layer chromatography identification analysis on the pair of test sample solutions, the negative blank sample solution of the phlegm-heat clearing injection without cornu gorais, the reference medicinal material solution and / or the reference substance solution, wherein in the step of thin-layer chromatography identification analysis, n-propyl alcohol, glacial acetic acid, ethanol and water are used as a developing solvent, and the volume ratio of n-propyl alcohol to glacial acetic acid to ethanol to water is (0.5-5): (0.5-5): (0.5-5): (0.5-5). According to the thin-layer identification method disclosed by the invention, a theoretical basis is provided for the quality control of the phlegm-heat-clearing injection and the deep research of a pharmacodynamic material basis by detecting the cornu gorais medicinal material in the phlegm-heat-clearing injection.

The invention discloses a thin-layer identification method for a honeysuckle medicinal material in a phlegm-heat-clearing preparation, which comprises the following steps: (1) pretreating the phlegm-heat-clearing preparation to obtain a test solution, and pretreating a reference medicinal material or a reference substance to obtain a reference medicinal material solution or a reference substance solution; and (2) carrying out thin-layer chromatography identification analysis on the pair of test sample solutions, the honeysuckle-lacking phlegm-heat clearing preparation negative blank sample solution, the reference medicinal material solution and / or the reference substance solution, wherein in the step of thin-layer chromatography identification analysis, an upper-layer solution of butyl acetate, formic acid and water is used as a developing solvent, and the volume ratio of butyl acetate to formic acid to water is (1-10): (1-10): (1-10). According to the thin-layer identification method disclosed by the invention, the honeysuckle medicinal material in the phlegm-heat-clearing preparation is detected, so that a theoretical basis is provided for the quality control of the phlegm-heat-clearing preparation and the deep research of a pharmacodynamic material basis.

The invention discloses a rhizoma belamcandae oral liquid for clearing pestilence and strengthening body resistance and a preparation method of the rhizoma belamcandae oral liquid, and the rhizoma belamcandae oral liquid for clearing pestilence and strengthening body resistance is prepared from the following raw material medicines: rhizoma belamcandae, honeysuckle flower, radix isatidis, folium isatidis, dandelion, herba eupatorii, radix bupleuri, radix astragali seu hedysari, glossy privet fruit and herba artemisiae scopariae. On the basis of the formula of the rhizoma belamcandae antiviral injection, astragalus membranaceus and glossy privet fruit are innovatively added to enhance the effect of supporting the healthy energy, a compound which simultaneously clears away heat and toxic materials and tonifies lung and qi is formed, and by adopting a modern extraction and inclusion technology and combining with a multi-wavelength HPLC fingerprint overall quality control method, the stable content of pharmacodynamic substance groups is ensured; the oral liquid preparation which is safe, convenient to take and suitable for preventing and treating respiratory virus infection is obtained.

The present invention discloses a multifunctional thermosensitive sustained-release hydrogel, its preparation method, and its use in the preparation of antipruritic and analgesic preparations. The present invention utilizes microspheres formed by a composite of hyaluronic acid and quaternary ammonium salt chitosan. These microspheres are sequentially mixed with a drug, a solubilizing agent-dissolved cooling substance, and poloxamer 407 using a simpler magnetic stirring method. This multifunctional thermosensitive sustained-release hydrogel is prepared without the use of a thickener. The hydrogel prepared by the present invention exhibits a strong cooling odor and sustained-release effect, along with good biocompatibility. When the drug used is an antipruritic and analgesic substance, such as oxymatrine, the hydrogel also has therapeutic and analgesic effects for non-histamine-dependent pruritus.

The present application relates to the technical field of analysis, in particular to a combined analysis method for analyzing the chemical components of Huangkui capsules, which is a fast, efficient and accurate method for analyzing the chemical components of Huangkui capsules based on UHPLC-Q-TOF / MS combined with MS-DIAL and GNPS strategy. By setting conditions such as positive and negative ion mode full scan, molecular ion peak pairing, accurate molecular weight mass error and isotope peak abundance, the compounds can be accurately identified, the molecular formula is generated, and the identification of the compounds is completed based on the self-built compound database. Further combined with MS-DIAL and GNPS, two molecular cages related to flavonoid structure are successfully identified according to MS / MS similarity, and a total of 13 compounds other than the self-built compound database are identified. It provides a useful reference for further development and utilization of Huangkui capsules.

The invention discloses a UHPLC Q-Exactive Orbitrap MS / MS (Ultra High Performance Liquid Chromatography Q-Exactive Orbitrap Mass Spectrometry / Mass Spectrometry) rapid identification method for chemical components and serum migration components of a compound radix scutellariae capsule for clearing heat from throat, which is characterized in that the chemical components and the serum migration components of the compound radix scutellariae capsule for clearing heat from throat are rapidly qualitatively identified by adopting UHPLC Q-Exactive Orbitrap MS / MS; 21 in-vitro components (including flavonoids, phenolic acids, triterpenes and the like) and 21 in-blood migration components (including 9 in-blood prototype components and 12 metabolites) are identified. The chemical components and serum migration components of the compound radix scutellariae capsule for clearing heat from throat are defined, a foundation is laid for fundamental research of pharmacodynamic substances of the preparation, and the blank of chemical research of serum drugs of the compound radix scutellariae capsule for clearing heat from throat is filled up.

The present invention provides a use of lactoferrin in combination with ergothioneine in the preparation of a drug for preventing and / or treating Alzheimer's disease. Compared with the use of lactoferrin or ergothioneine alone, in the present invention, the combined use of lactoferrin and ergothioneine at a specific ratio as an active pharmaceutical ingredient can reduce cell damage caused by Aβ25-35, reduce the expression of a p-Tau protein, lower the oxidative stress level and regulate apoptosis, alleviate memory impairment and cognitive dysfunction, and can reduce Aβ deposition in mouse plasma.