122 results about "Pharmacological Substance" patented technology

The invention relates to the technical field of compound traditional Chinese preparations, and in particular relates to a quality control method of liuwei wuling tablets. The quality control method is characterized in that 20 different proportion groups are formed by randomly sampling six medicinal materials of the liuwei wuling tablets; a corresponding HPLC fingerprint spectrum is established, and in-vitro anti-hepatic fibrosis activity is measured; and an SPSS software is utilized to analyze, so that a pectrum-effect relationship of in-vitro anti-hepatic fibrosis effect of the liuwei wuling tablets is established. The relatively perfect quality control method is established on the basis of pharmacodynamic substances of the liuwei wuling tablets. Compared with a conventional single-index content measuring method, the quality control method is more comprehensive, scientific and standard, and has the advantages that the content of multiple components is simultaneously measured, so that not only the analysis time is shortened, but also the loss of a solvent is saved.

The invention discloses a method for evaluating chemical composition of Rosa xanthina on the basis of antithrombotic spectrum-effect relationship. The method comprises the following steps: preparing extract of different polar components of the Rosa xanthina with a modern separation technology; establishing fingerprint of extract of each component with high-performance liquid chromatography, and calibrating characteristic peaks; evaluating antithrombotic activity of different extract on the basis of platelet aggregation inhibition rate, prothrombin time, thrombin time and activated partial thromboplastin time as indexes; substituting fingerprint characteristic peak data and pharmacodynamical activity data into a mathematical model for spectrum-effect correlation analysis, and evaluating pharmacodynamical activity of the characteristic peaks. With adoption of the method for evaluating the chemical composition of the Rosa xanthina on the basis of the antithrombotic spectrum-effect relationship, the antithrombotic chemical composition in the Rosa xanthina can be evaluated rapidly and accurately, a scientific and effective method is provided for research of pharmacodynamic material basis and quality control of the Rosa xanthina, and reference is provided for further development of Rosa xanthina drugs or health care products for treating thrombotic diseases.

The invention relates to the field of pig feed, in particular to pig feed capable of improving the meat quality, and a preparation method thereof. The pig feed capable of improving the meat quality mainly comprises the following raw materials: corns, bean pulp, oat, fish meal, rapeseeds, fructus forsythiae, honeysuckle flowers, cyrtomium fortune, radices sileris, radix bupleuri, astragalus membranaceus, bighead atractylodes rhizome, poria cocos, dried orange peel, Chinese angelica, white paeony root, dandelion, motherwort, purslane, codonopsis pilosula and feed additives. The feed additives comprise glycerol monolaurate, calcium propionate, amino acid and sodium molybdate. The pig feed capable of improving the meat quality, which is provided by the embodiment of the invention, has multiplefunctions of reinforcing the vital essence and strengthening the primordial qi, regulating physiological function, improving immunity, resisting inflammation, resisting bacteria, resisting viruses and the like through the proportioning of nutritional substances, pharmacological substances, amino acid and various cereals, increases absorption and utilization of nutritional components by pigs and enables the meat to be delicious.

The invention discloses a pharmacodynamic material basis screening method of artemisia capillaries soup, relates to a pharmacodynamic material basis screening method of a traditional Chinese medicine, and solves the problems of long time cycle, large consumption and high cost of screening of effective components of the artemisia capillaries soup in the prior art. The pharmacodynamic material basis screening method is as follows: 1, establishment of peak area of body components of the artemisia capillaries soup; 2, establishment of metabonomics biological markers of the artemisia capillaries soup on the basis of CCL4 liver injury model; and 3, establishment of plotting of correlation between marker metabolites and serum constituents (PCMS) research method. The invention provides the pharmacodynamic material basis screening method of the traditional Chinese medicine, the pharmacodynamic material basis screening method is fast, simple, accurate, low-cost, small in consumption, and better in versatility, can effectively determine the efficacy-related components, can reflect the efficacy index comprehensive effect characteristic of multi components, and can be used for pharmacodynamic material basis screening.

The invention provides a medicament for treating infectious diseases, a preparation method and the application thereof. The medicament is prepared from scutellaria baicalensis, jasmine, erigeron breviscapus and cholic acid, and has the following effects: by aiming at the central link--inflammatory reaction of an infectious disease, removing harmful substances such as inflammatory mediators, free radicals and the like, and improving the local microcirculation state, thereby alleviating the pathological damages caused by inflammations; meanwhile, the medicament has the function of regulating the overall internal environment, and can effectively treat lower respiratory tract infection and acute lung injury induced by infection; and the effective substances and functional mechanism of the medicament are relatively clear.

The invention provides a preparation method of a smilax rhizome extract, the smilax rhizome extract prepared by the method and application of the smilax rhizome extract to preparation of medicines for preventing, relieving and treating inflammation. The content of total saponin ingredients in the smilax rhizome extract is high, the total saponin ingredients account for over 50% of the weight of the smilax rhizome extract, pharmacodynamics tests prove that the therapeutic effect of the total saponin ingredients is remarkable, the total saponin ingredients are a principal medical effect material basis for treating gynecological inflammation through smilax, and can serve as a raw material of new drugs for effective part of traditional Chinese medicines.

The invention provides a preparation method for Chuzhou chrysanthemum extract powder according to the characteristics of effective constituents contained in Chuzhou chrysanthemum, and the preparation method aims at a plurality of effective constituents of the Chuzhou chrysanthemum. A rotary evaporator is adopted to perform double extraction on the Chuzhou chrysanthemum, extracts of volatile oil and acetic acid are obtained, and then decoction dregs are added into ethanol water to perform extraction again with ultrasonic. Beta-cyclodextrin wrapping is performed on the volatile oil to obtain wrappage. Acid extract solution is concentrated, alcohol extract solution is concentrated after alcohol is recovered, two concentrated solutions are merged, and freeze-dried powder is obtained after the two concentrated solutions are frozen and dried. Eventually, the wrappage and the freeze-dried powder are porphyrized and mixed to obtain the Chuzhou chrysanthemum extract powder. According to the preparation method for the Chuzhou chrysanthemum extract powder, the extract method is selected aiming at the characteristics of the plurality of effective constituents of the Chuzhou chrysanthemum, and each effective substance is retained to the maximum extent; extraction time is short, the temperature for the whole process is smaller than or equal to 80 DEG C, damage to efficacy material is avoided, and the preparation method is high in efficiency, time-saving and labor-saving; and the extract powder can be used in place of the Chuzhou chrysanthemum, and can also serve as raw material for production of medicine and health care products.

The invention provides a drug network pharmacology intelligent and quantitative analysis method and system based on a network target original theory, wherein the method and system are used for measuring the overall effect of intervening a disease biological network by drugs (including various drug types such as Chinese and western drugs). The method can integrate qualitative and/or quantitative biological information related to diseases and drugs, takes the disease biological network as a target and realizes effect measurement of drug intervention on the disease biological network from the system and overall perspective, and reveals the overall action mechanism of the drugs. The method provides a qualitative and quantitative selectable measurement mode, measures the drug intervention network effect by adopting biological function multi-scale qualitative analysis and/or time-space multi-dimensional quantitative analysis, and provides a new key technique for breaking through the limitation of a traditional experience-based or single-target-based drug research mode, understanding a drug network regulation mechanism, and quickly and intelligently discovering pharmacodynamic substances, action mechanisms, curative effect objective indexes and clinical indications and the like.

The inventive method for automatically analysing a cellular sample consists (a) in colouring the cell nucleuses of a sample, (b) inobtaining at least one digital picture of the sample, and (c) in digitally analysing said image and is characterised in that the cell nucleus colouring stage (2) is embodied in the form of a DNA-non-specific colouring. In order to use the inventive method, said invention also relates to a method for selecting a culture medium, a method for measuring substance toxicity and to a method for measuring the cytopathological characteristics of a virus. A method for selecting pharmacological substances for treating excess weight and obesity diseases and a method for selecting substances for treating osteoporosis are also disclosed.

The invention discloses application of a cinnamon polyphenol composition. The cinnamon polyphenol composition can be applied to preparation of drugs and/or medical instruments for prevention and/or treatment of virus infection diseases. The invention discloses antiviral activity of the cinnamon polyphenol composition. Based on an intermediate infrared spectrum fingerprint and total tannin and total sugar content determination technology, a quality control method of the cinnamon polyphenol composition is established. Study confirms that the cinnamon polyphenol composition meeting the quality control requirement of the invention has significant antiviral activity. The cinnamon polyphenol composition provided by the invention has the characteristics of basically clear pharmacodynamic substances, controllable quality and stronger efficacy, and adapts to the process technology and quality standardization requirements of modern manufacturing industry.

The invention relates to a pharmacological substance based on biologically active substances obtained from the plant Tribulus terrestris L. to be used as an agent reducing blood sugar, improving blood circulation and especially blood circulation in veins and capillaries of the limbs of diabetic patients, reducing bad cholesterol level, increasing good cholesterol concentration, maintaining cardiovascular and liver functioning with an additional prophylactic or healing effect on the immune system and the immune resistance. The pharmacological substance represents a combination of bio-active trivalent chromium and a complex of steroid saponins obtained from the plant Tribulus Terrestris l. and consisting of furostanols, spirostanols, sapogenins, sterols, flavonoids and other biologically active substances typical of this plant.

The invention provides a preparation method and application of compound ketoconazole ointment. The preparation method utilizes medicinal effective components such as ketoconazole, metronidazole and menthol and aids such as anhydrous sodium sulfite, a surfactant and hydrochloric acid as raw materials. Through adjustment and optimization of the preparation method, the compound ketoconazole ointmenthas stable performances and good bactericidal performances, effectively treats pet skin diseases and can be further used in preparation of a drug containing ketoconazole or metronidazole.

The invention provides a method for identifying the chemical components in the Xuebijing injection. The ultra-high performance liquid chromatography-ion mobility-quadrupole time-of-flight mass spectrometry is combined, and the identification of multiple types of chemical components in the Xuebijing injection can be realized by reasonably selecting chromatographic conditions and mass spectrometry conditions; and the method has the advantages of simplicity, convenience, high sensitivity, high analysis speed, strong specificity and the like, and provides a basis for further researching the pharmacodynamic material basis of the Xuebijing injection.

The invention discloses digalactosyldiacylglycerol with a structure shown as a formula (I), a preparation method of the digalactosyldiacylglycerol and a purpose of the digalactosyldiacylglycerol for preparing a PPAR alpha agonist, or a PPAR gamma agonist and PPAR alpha/gamma double-agonists. The formula (I) is shown as the accompanying drawing, wherein R1 and R2 represent acyl fragments; the acyl fragments are straight-chain or branch-chain fatty acid containing 1 to 30 carbon atoms; in addition, 0 to 15 cis-form or trans-form double keys are contained in the acyl fragments. The agitation effect of digalactosyldiacylglycerol on PPAR alpha and PPAR gamma and the duplex agitation effect on PPAR alpha/gamma are discovered; the application value of the compound of the type to the prevention and treatment of PPARs relevant diseases is disclosed. In addition, active ingredients of alga beneficial to the cardiovascular health are discovered and separated; the medicine effect substances and functional factors are provided for developing alga medicine, health care food and food.

The invention discloses an analysis method for researching a fingerprint spectrum of a Mailuoning injection based on UPLC-DAD-MS. On the basis of research on early-stage chemical components, the UPLC-DAD-MS liquid chromatography-mass spectrometry fingerprint spectrum of the Mailuoning injection is established, and 23 main components including 13 organic acids, 1 coumarin, 2 iridoid, 1 flavone, 1 lignan, 3 saponins and 2 other components can be directly analyzed and identified. According to the method, optimized chromatographic conditions can be rapidly obtained; therefore, the optimal fingerprint spectrum is obtained, reproducibility is good, the relative retention time and the relative peak area variation are both less than 5%; the main chemical component composition of the Mailuoning injection is accurately illustrated, which is of great significance to illustration of the pharmacodynamic material basis of the Mailuoning injection, and lays a foundation for improvement of the qualitycontrol standard of the Mailuoning injection, establishment of a scientific and reasonable quality control method and optimization of a preparation process.

The invention provides a method for evaluating quality of Herba Cirsii Setosi through quantitative analysis of multicomponents by a single marker. According to the method for evaluating the quality ofthe Herba Cirsii Setosi through the quantitative analysis of multicomponents by the single marker, firstly, a HPLC content determination method for four main medicinal components in the Herba CirsiiSetosi is established; rutin is used as an internal reference substance, relative correction factors of another three active components are calculated, and the system suitability and the method reproducibility of the relative correction factors are investigated; chromatographic peak positioning is carried out according to relative retention time, the relative correction factors are combined for calculating the content of all to-be-detected components, through the mutual verification of the quantitative analysis of multicomponents by the single marker and an external standard method, it is proved that no significant difference exists in determination results; the problems that reference substances are high in costs and are not easy to obtain are solved, the content of index components is calculated through the relative correction factors and the chromatographic peak positioning, and the simultaneous determination of multiple medicinal substances in the Herba Cirsii Setosi is realized; and the costs can be saved, the operation can be simplified, the efficiency is improved, the detection sensitivity is high, the stability is good, the determination results are exact and reliable, andthe method has great significance for ensuring the quality control and the clinical effects of the Herba Cirsii Setosi.

The invention discloses a preparation process of a heat-clearing and detoxifying veterinary traditional Chinese medicine. The veterinary traditional Chinese medicine is prepared from the following components in parts by weight: 3-6 parts of folium isatidis, 5-7 parts of radix isatidis, 5-7 parts of gypsum, 5-6 parts of rheum officinale, 4-5 parts of compound of glauber-salt and liquorice, 4-6 parts of pericarpium citri reticulatae, 4-6 parts of astragalus membranaceus, 5-7 parts of scutellaria baicalensis, 3-5 parts of cinnamon, 3-6 parts of fried yam, 1-3 parts of codonopsis pilosula, 3-5 parts of Lysimachia christinae hance, and 3-5 parts of schizonepeta. By using a freeze dryer provided by the invention, the problems that an existing vacuum heating drying method enables active pharmaceutical substances with poorer heat resistance to lose activity, and volatile active pharmaceutical substances are easy to volatilize and lose in the heating drying process can be solved.

The invention discloses a detection method of a Chinese herbal compound containing angelica sinensis. The method comprises the following steps: taking a Chinese herbal compound test sample and reference substances for detection, wherein the Chinese herbal compound comprises angelica sinensis, cassia twig, licorice, white peony root, ginger and jujube; the reference substances are gallic acid, paeoniflorin, ferulic acid, liquiritin, ammonium glycyrrhizinate and 6-gingerol; and the chromatographic conditions of the detection are as follows: a C18 chromatographic column is adopted, methanol or acetonitrile is used as a mobile phase A, an acid aqueous solution is used as a mobile phase B, and a gradient elution procedure includes that 5%-12% A for 0-20 min is performed; 12%-85% A for 20-90 min is performed; a flow velocity is 0.8-1.2 mL/min; a column temperature is 25-40 DEG C; and a detection wavelength is 210-330 nm; and obtaining component information or component and content information of the Chinese herbal compound according to a detection result. According to the invention, the chemical components of the Chinese herbal compound containing the angelica sinensis are comprehensively and systematically analyzed by applying a high performance liquid chromatography technology, and a theoretical basis is provided for deep research on quality control and a pharmacodynamic material basis.

The invention discloses a method for establishing a fingerprint spectrum of a lung-ventilating and toxin-vanquishing prescription. The method comprises the following steps of: calculating RSD values of relative retention time and relative peak areaof each common peak by taking naringin as a standard substance; according to the invention, the fingerprint spectrum of the lung-ventilating and toxin-vanquishing prescription is established through the UHPLC method, and the contents of the nine effective components are determined, so that reference is provided for research on pharmacodynamic material basis and quality control of the lung-ventilating and toxin-vanquishing prescription.

The present invention provides a non-clottable matrix on a solid support comprising immobilized and crosslinked fibrinogen. The matrix may further comprise, in and/or on the matrix, one or several biologically active compound(s) and/or pharmacological substance(s). The matrix may be composed of one or several fibrinogen layer(s). The solid support according to the present invention may be selected from the group consisting of orthopaedic devices, implants, stitches, stents, pins, screws, plates, and sutures.

The invention discloses a method for evaluating the quality of gnaphalium affine by a quantitative-analysis-of-multi-components-by-single-marker (QAMS) method. The method comprises the steps as follows: firstly, an HPLC content determination method for four main medicinal components in the gnaphalium affine is established; chlorogenic acid is used as an internal reference substance; relative correction factors of other three active components are calculated; the system applicability and the method reproducibility of the relative correction factors are inspected; chromatographic peak positioning is performed according to relative retention time; the content of each component to be determined is calculated in combination with the relative correction factors; and content determination resultsare proved to have no obvious difference by virtue of mutual verification of the QAMS method and an external standard method. The method disclosed by the invention solves the problems that the reference substance is high in cost and difficult to obtain; by virtue of the relative correction factors and the chromatographic peak positioning, the content of index components is calculated; synchronousdetermination of various medicinal substances in the gnaphalium affine is realized, so that the cost can be reduced, the operation is simplified, and the efficiency is improved; the method is high indetection sensitivity and high in stability; the determination results are accurate and reliable; and the method has a great significance on the quality control of the gnaphalium affine and the guarantee of the clinical effects.

The invention relates to a method for carrying out quality control on platycodon grandiflorum (a traditional Chinese herb) based on bioactivity assay, which effectively realizes the quality control on platycodon grandiflorum and ensures the safe and effective clinical application of platycodon grandiflorum. The method comprises the following steps: crushing platycodon grandiflorum, and carrying out ultrasonic extraction on the crushed platycodon grandiflorum by using ethanol so as to obtain a dry matter; carrying out multiple proportion diluting on the obtained dry matter into seven groups by using normal saline according to an initial concentration at an agent distance of 0.5, thereby obtaining samples; preparing the platycodon grandiflorum into working reference substances according to the sample steps; dissolving phenol red by using a sodium bicarbonate solution, then making a standard curve through taking the dose of the phenol red as a x-coordinate and taking an OD (optical density) value as a y-coordinate; taking animals as experimental objects, grouping the animals, carrying out drug administration respectively, and putting the animals subjected to drug administration to death; then taking tracheas of the animals and putting the tracheas into normal saline, centrifuging and then taking supernate, determining the OD value at a wavelength of 546 nm, and calculating and comparing the secreted phenol-red content of the tracheas according to the phenol-red standard curve; and with the phenol-red content as an index, carrying out potency calculation. The method disclosed by the invention is easy to operate, stable, reliable, accurate in test and good in quality control effect, and can provide new ideas and references for the quality control and quality evaluation of other traditional Chinese herbs with undefined pharmaceutical active substances.

The invention provides a method for establishing a fingerprint spectrum of a bactericidal itching-relieving lotion. The method comprises the following steps: S1, preparing a mixed reference substancesolution, namely precisely weighing matrine, astilbin, phellodendrine, berberine hydrochloride, cnidium lactone and atractylodes lancea reference substances respectively, and adding methanol to prepare the mixed reference substance solution; S2, preparing a bactericidal itching-relieving lotion sample solution; S3, preparing a single reference medicinal material solution; and S4, determining. Thefingerprint spectrum calibrates 16 common peaks and determines the five medicinal materials belonging to a prescription, the established fingerprint spectrum has high technical content, and the singleness and one-sidedness of the existing quality control method are avoided. An effective quality control basis is provided for the production of the bactericidal itching-relieving lotion, and a foundation is laid for the research on the correlation between the pharmacodynamic material basis and the pharmacological action of the bactericidal itching-relieving lotion.