157 results about "Pharmacological Substance" patented technology

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine prescription efficacy quantification method and system.The method comprises the steps that chemical component spectrums, pharmacodynamic substance contents and biological activity indexes of traditional Chinese medicines forming a prescription are obtained; constructing a multi-target regulation and control network by combining a traditional Chinese medicine-target interaction database according to the chemical component spectrum, the pharmacodynamic substance content and the biological activity index, identifying a core efficacy pathway cluster by adopting a Louvain algorithm, and calculating a pathway activation intensity value by adopting a pathway target membership weighting model; according to the core efficacy pathway cluster, establishing a mapping relation between the core efficacy pathway cluster and traditional Chinese medicine efficacy terms through ontology and semantic mapping, and according to pathway activation intensity values, generating a prescription efficacy intensity vector through a weighted aggregation model; according to the efficacy intensity vector of the prescription, verifying and correcting the network weight through an in-vitro cell model, and generating the efficacy quantification of the prescription in combination with the clinical syndrome calibration coefficient. Therefore, the problems of single data dimension and insufficient quantification result accuracy in the prior art are solved.

The invention discloses a quality control method of a traditional Chinese medicine preparation based on an IV-catfish orifice-freeing prescription, and relates to the technical field of analysis of traditional Chinese medicine preparations, and the quality control method comprises the following steps: carrying out microscopic identification on traditional Chinese medicine components in the IV-catfish orifice-freeing prescription, and determining a pharmacodynamic material basis. According to the quality control method for the traditional Chinese medicine preparation based on the IVV orifice-freeing prescription, various evaluation means such as a drug effect equivalent evaluation index, a stability test, a quality test and a clinical test are comprehensively applied, the consistency and reliability of the curative effect of the traditional Chinese medicine preparation can be remarkably improved, and the drug effect equivalent evaluation index is analyzed from the two factors of drug effect intensity and action time; the method ensures the equivalence of the medicinal component group and the original traditional Chinese medicine preparation, comprehensively detects the raw material medicines and the preparation, including characters, identification, inspection and content determination items, and ensures that the quality of each batch of samples meets the standard, so that the curative effect of the traditional Chinese medicine preparation is effectively guaranteed, and the efficacy of the traditional Chinese medicine preparation is improved.

The invention discloses a construction method of a gout compound decoction fingerprint as well as the fingerprint and application thereof, and belongs to the technical field of analysis and detection. The construction method of the fingerprint spectrum comprises the following steps: taking a test solution prepared from the gout compound decoction; the test solution is subjected to HPLC-SPD detection, so that the fingerprint spectrum of the gout compound decoction is obtained, and the HPLC detection conditions are that a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, and a C18 chromatographic column is used for detecting the fingerprint spectrum of the gout compound decoction. An aqueous solution containing 0.1%-0.2% of phosphoric acid is used as a mobile phase A, and acetonitrile is used as a mobile phase B for gradient elution; the flow velocity is 0.8-1 mL / min, the column temperature is 25 DEG C, the sample injection volume is 5-10 [mu] L, and a diode array detector is 190-400 nm full-wave band scanning. The chemical components in the gout compound decoction are comprehensively and systematically analyzed, and a basis is provided for quality control and in-depth study of a pharmacodynamic material basis.

The invention relates to the technical field of traditional Chinese medicine research, in particular to a discovery method of a cough relieving and phlegm eliminating quality marker of cough relieving granules and the quality marker. According to the application, the cough-relieving and phlegm-eliminating drug effects of the cough-relieving granules are evaluated by virtue of concentrated ammonia water cough inducing and phenol red excretion tests, plasma pharmaceutical chemistry research of the cough-relieving granules is developed by virtue of a UHPLC-Q-Exactive Plus Orbitrap HRMS technology, and 26 prototype blood-entering components of the cough-relieving granules are analyzed and identified; through network pharmacological analysis of components entering blood, 11 potential active components for relieving cough and eliminating phlegm of the cough-relieving granules are screened, relate to 40 common targets such as IL6, TLR4, STAT3 and the like, and mainly participate in signal channels such as PI3K-Akt, HIF-1, EGFR (epidermal growth factor receptor) and the like; the seven prototype blood-entering components are quantitatively analyzed by utilizing pharmacokinetics. The three compounds of azelaic acid, caffeic acid and vanillin are used as the cough relieving and phlegm eliminating quality markers of the cough relieving granules through effectiveness, transitivity and testability. The research result is of great significance in deeply defining the pharmacodynamic material basis of the cough-relieving granules, optimizing the quality standard and promoting the clinical application of the cough-relieving granules.

The invention provides a large and small model collaborative traditional Chinese medicine prescription optimization method and system, a terminal and a storage medium, and the method comprises the steps: firstly determining a prescription needing to be optimized, sorting related public literatures into an input document, constructing a local prescription knowledge base, and carrying out question answering based on a prescription optimization large language model to obtain a large model optimization prescription; then, determining related pathway targets of applicable symptoms in the prescription needing to be optimized, collecting chemical substances in the prescription needing to be optimized to obtain a prediction result of a pharmacodynamic substance level, performing medicinal material tracing analysis on the prediction result, optimizing prescription composition based on pharmacodynamic substance weight to obtain a small model optimized prescription, and determining the pharmacodynamic substance weight based on the small model optimized prescription. And performing comprehensive analysis based on the large model optimization prescription and the small model optimization prescription to generate final prescription optimization recommendation. The efficient traditional Chinese medicine prescription composition optimization method is constructed from the macroscopic level and the microscopic level, and the scientificity and the practicability of prescription optimization can be improved.

The present application discloses a method for determining the content of four active ingredients in Yanli Shuang oral dripping pills in the field of drug detection technology. A method for simultaneously detecting the content of four active ingredients, camphor, menthone, borneol and menthol in Yanli Shuang oral dripping pills, is established by using the GC-MS internal standard method. The method is accurate, simple and has good repeatability, and can be used for the quality control of Yanli Shuang oral dripping pills. The present invention also uses GC-MS to establish a fingerprint of Yanli Shuang oral dripping pills, which has the advantages of saving time and high sensitivity, and provides a scientific basis for clarifying the material basis of drug efficacy and quality control based on volatile components.

The invention relates to a technology for shortening the aging time of dried orange peel, and provides a method for improving nobiletin and hesperetin by co-fermenting dried orange peel with two microorganisms, which comprises the following steps: preparing a mixed bacteria solution by using Aspergillus sp. And Exophiala similia; mixing the sterilized pericarpium citri reticulatae powder and the sterilized rice meal to serve as a fermentation substrate, adding the mixed bacterial liquid and the sterilized water into the fermentation substrate to serve as a reaction system, fully stirring the reaction system, and placing the reaction system in a constant-temperature incubator for fermentation. By adopting the method disclosed by the invention, the contents of nobiletin and hesperetin in the dried tangerine or orange peel can be efficiently increased within a relatively short time, the aging time of the dried tangerine or orange peel is shortened, and the conversion efficiency of medicinal effect substances in the dried tangerine or orange peel is improved.

The invention relates to a construction method and application of a bile acid fingerprint spectrum in bile, and relates to the technical field of traditional Chinese medicine detection. The construction method comprises the following steps: preparing a reference substance solution: weighing at least two of taurocholic acid, taurodeoxycholic acid, glycocholic acid, cholic acid and glycodeoxycholic acid reference substances, and preparing the reference substance solution; preparing a test sample solution, namely filtering bile, taking filtrate, heating in a gradient manner, drying and grinding to obtain a test sample, and preparing the test sample solution; and constructing a fingerprint spectrum: respectively injecting the reference substance solution and the test solution into a high performance liquid chromatograph, and determining under a predetermined liquid phase condition to construct the bile acid fingerprint spectrum. The construction method can be used for fully and effectively separating and identifying various bile acid components in the bile, can be used for simultaneously detecting and measuring the various bile acid components in the bile, and has important significance in the aspects of quality control, authenticity identification, basic research on pharmacodynamic substances and the like of the bile.

The present invention discloses a γ-arylbutenolide compound, a preparation method thereof, and an application thereof in the preparation of cardiovascular protective drugs. The above-mentioned structurally novel γ-arylbutenolide compound is isolated for the first time from the fermentation culture of the marine herbal soft coral symbiotic fungus Aspergillus sp. EGF7-0-1, and it is found for the first time that the above-mentioned compound has a protective effect on the cardiovascular system, providing a pharmacodynamic substance basis for the subsequent research on cardiovascular diseases.

The invention is applicable to the technical field of detection of Yankingning tablets, and particularly relates to a fingerprint detection method for Yankingning tablets, which comprises the following steps: preparing a Yankingning tablet test solution; preparing a reference solution; after the chromatographic conditions are matched with the system applicability, respectively injecting 5 microliters of the test solution and 5 microliters of the reference solution into a liquid chromatograph for determination to obtain a fingerprint spectrum and a reference fingerprint spectrum of the Yankingning tablets; and analyzing according to the fingerprint spectrum of the Yankingning tablet and the control fingerprint spectrum to obtain similarity information. According to the method, through a standardized detection process, various components in the Yankingning tablet are detected at the same time, the basis of pharmacodynamic substances of the Yankingning tablet is comprehensively reflected, and the quality of the Yankingning tablet can be comprehensively evaluated; by comprehensively analyzing the similarity between the fingerprint spectrum of the Yankingning tablets and the control fingerprint spectrum, the quality and consistency of the Yankingning tablets can be comprehensively evaluated, and the stability and reliability of quality evaluation of the Yankingning tablets are improved.

The invention relates to the technical field of traditional Chinese medicine analysis, and discloses a codonopsis pilosula root traditional Chinese medicine in-blood pharmacodynamic substance mass spectrometry imaging method and application thereof, and the method comprises the following steps: S1, pharmacodynamic research and serum drug chemical analysis of codonopsis pilosula treatment diseases: (1) establishment of an animal model: taking a spleen deficiency model as an example, (2) serum drug chemical analysis, and (3) establishment of an animal model: taking a spleen deficiency model as an example; and S2, researching the distribution of the blood-entering components in the traditional Chinese medicine. Differential distribution of various medicinal effect substances in different tissues of the plant is accurately identified and positioned by utilizing a mass spectrum imaging technology, and deep mass spectrum imaging analysis is quickly performed on the tissues of the target micro-region, so that the type and content information of the components entering the blood is obtained; according to the technology, space metabonomics is added, and the whole action mechanism of the codonopsis pilosula for treating diseases is explored, including codonopsis pilosula pharmacodynamics research and searching of pharmacodynamic substance components and spatial distribution of the pharmacodynamic substance components in codonopsis pilosula roots.

The invention discloses a method for mining a key path of a traditional Chinese medicine efficacy substance group and a efficacy index group based on PLS-SEM, and the method comprises the steps: building a PLS-SEM model based on the experimental data of the efficacy substances and efficacy indexes of the traditional Chinese medicine, selecting a plurality of efficacy substances in the traditional Chinese medicine as independent variables, selecting a plurality of efficacy indexes as dependent variables, optimizing the variable grouping, and obtaining the key path of the efficacy substance group and the efficacy index group of the traditional Chinese medicine; and establishing a causal path model of reflection type reflection type forming or forming type reflection type forming. By adopting the method to discuss the pharmacodynamic data of different traditional Chinese medicine formulas, pharmacodynamic substance groups with similar efficacy, pharmacodynamic index groups with similar characterization and optimal paths of pharmacodynamic substances acting on pharmacodynamic indexes can be mined, and experiments show that the method is feasible and effective, supports are provided for clarification of traditional Chinese medicine principles, and references are provided for development of new drugs.

The present invention relates to the use of TDP1 and eltrombopag in the treatment of myotonic dystrophy type 2. The present invention uses a fruit fly model to identify that TDP1 knockdown can effectively rescue neurodegeneration in the CCTG repeat fruit fly model, reduce pigment production block, cell death and ommatidium fusion, and improve motor defects. At the same time, in vitro screening using small molecules found that eltrombopag can be used as a TDP1 inhibitor and can significantly reduce disease cytotoxicity. The present invention provides a new target and pharmacological substance for CCTG repeat expansion diseases in myotonic dystrophy type 2, and expands the medical use of eltrombopag. The present invention provides a new drug with clinical application prospects for the treatment of CCTG repeat expansion diseases in myotonic dystrophy type 2.

The invention discloses a method for detecting anions in a phlegm-heat-clearing injection, the detection method comprises the following steps: a test solution and a reference solution are taken for detection, the phlegm-heat-clearing injection is composed of scutellaria baicalensis, bear gall powder, cornu gorais, honeysuckle, fructus forsythiae and propylene glycol; chromatographic conditions of the detection are as follows: an anion exchange chromatographic column is adopted, an eluent is an acid aqueous solution, an alkali aqueous solution and / or a buffer salt aqueous solution, the flow velocity is 0.1-2.0 ml / min, the column temperature is 20-40 DEG C, a detector is an electrical conductivity detector, the detection mode is suppression electrical conductivity detection, and the sample size is 10-40 [mu] l; and obtaining the content information of the anions in the phlegm-heat clearing injection according to the detection result. The method disclosed by the invention is simple, convenient, efficient and rapid, and provides a theoretical basis for the quality control of the phlegm-heat clearing injection and the deep research of a pharmacodynamic material basis by detecting the content of the anions.

The invention discloses a fingerprint spectrum construction method of a traditional Chinese medicine composition containing angelica sinensis and cinnamon. The construction method comprises the following steps: preparing a test solution; preparing a reference substance solution; and obtaining the fingerprint spectrum of the traditional Chinese medicine composition according to results of high performance liquid detection of the test solution and the reference solution. The chemical components of the traditional Chinese medicine composition containing angelica sinensis and cinnamon are comprehensively and systematically analyzed, and a theoretical basis is provided for deep research on quality control and pharmacodynamic material basis.

The invention discloses a substance basis and a detection method for the drug effect of liver-bean jelly, and belongs to the technical field of medicines. In-vitro chemical components of liver-bean jelly are combined to track blood entering components of liver-bean jelly and carry out attribution analysis; obtaining an action target spot of the liver-bean linging for intervening the WD hepatic fibrosis; screening metabolic markers for significant callback of liver-bean linging, and performing enrichment analysis to obtain a significant metabolic pathway; constructing a'small molecule metabolite-reaction-enzyme-target 'composite metabolic network by the action target and the significant callback metabolic marker, and screening a core action target, a core metabolic marker and a core metabolic pathway; a molecular docking technology is used for screening a liver-bean drug effect material basis, and a multi-dimensional network of the liver-bean drug effect material basis, a core action target, a core metabolic marker, a core metabolic pathway and WD liver fibrosis is visualized. The invention discloses a pharmacodynamic material basis and a metabolic regulation mechanism for treating WD hepatic fibrosis by using liver bean paste.

The present application discloses a method for constructing a fingerprint of a gout compound decoction and its fingerprint and application, and belongs to the field of analytical detection technology. The method for constructing the fingerprint comprises the following steps: taking a test solution prepared from a gout compound decoction; performing HPLC-SPD detection on the test solution to obtain a fingerprint of the gout compound decoction, wherein the high performance liquid chromatography detection conditions are: using a C18 chromatographic column; using an aqueous solution containing 0.1% to 0.2% phosphoric acid as mobile phase A, and acetonitrile as mobile phase B for gradient elution; the flow rate is 0.8 to 1 mL / min, the column temperature is 25 ° C, the injection volume is 5 to 10 μL, and the diode array detector is scanned at 190-400 nm full band. The present application conducts a comprehensive and systematic analysis of the chemical components in the gout compound decoction, providing a basis for in-depth research on quality control and the basis of pharmacological substances.

The invention relates to a purification and application method of a self-assembled supramolecular substance based on a radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root decoction, which comprises the following steps: proportioning radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root, adding water, decocting to obtain a GQD decoction, sequentially carrying out ultrafiltration and alcohol precipitation treatment on the supernatant of the GQD decoction, volatilizing the obtained supernatant, redissolving, and freeze-drying to obtain the self-assembled supramolecular substance, and purifying to obtain the self-assembled supramolecular substance based on the self-assembled supramolecular substance based on the radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root decoction. The compound is further used for preparing anti-inflammatory and anti-UC drugs; according to the invention, the anti-UC characteristic of the traditional Chinese medicine composition is utilized to recover intestinal flora with UC disorder and repair damaged intestinal barriers, meanwhile, core pharmacodynamic substances are reserved to the greatest extent, other irrelevant or harmful impurities can be reduced, and the traditional Chinese medicine composition is suitable for separating and purifying a nano-scale supramolecular compound with biological activity in a traditional Chinese medicine decoction to improve the curative effect and reduce toxic and side effects, so that the traditional Chinese medicine composition is suitable for clinical application. And the intestinal micro-ecology and mucous membrane barrier function can be repaired.

The invention provides rhizoma alismatis purified polysaccharide as well as a preparation method, an identification method and application thereof, the rhizoma alismatis purified polysaccharide is a functional component with a novel structure in a traditional Chinese medicine rhizoma alismatis water extract obtained by further extraction and purification, the administration efficiency is effectively improved, and the pharmacodynamic substances are clarified; in-vivo and in-vitro experiments show that the polysaccharide can improve the antioxidant capacity of the liver and relieve inflammatory response; synthesis and metabolism of liver bile acid can be adjusted, cholestasis of the liver is relieved, and bile acid circulation is promoted so as to relieve acute liver injury caused by acetaminophen; the effect of promoting the recovery of liver injury patients is obvious; and the compound also can be used as prebiotics to improve the homeostasis of intestinal microorganisms and promote the synthesis of short-chain fatty acids so as to have beneficial effects on the liver.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

The application relates to the technical field of pharmaceutical analysis, and discloses a pharmacokinetic analysis method of Tongwei Xiaozheng medicinal liquid, which adopts Q-Orbitrap high-resolution liquid chromatography-mass spectrometry to analyze the concentration of Jasmonic acid in blood components of the Tongwei Xiaozheng medicinal liquid in rat plasma, establishes a plasma concentration determination method for the blood original component Jasmonic acid, carries out methodological verification such as specificity, precision and accuracy, and then calculates pharmacokinetic parameters. In the Tongwei Xiaozheng medicinal liquid, 92 blood components are identified, 17 of which are original components and 75 of which are metabolites, the application firstly determines that Jasmonic acid is one of the blood original components of the Tongwei Xiaozheng medicinal liquid, and reveals the pharmacokinetic characteristics of the Jasmonic acid in normal and model rats. The method has strong specificity, high sensitivity and good reproducibility, and can provide a scientific basis for the research on the pharmacodynamic material basis of the Tongwei Xiaozheng medicinal liquid, the establishment of quality control standards and the clinical rational use of drugs.

The invention provides an ultra-high performance liquid chromatography-quadrupole rod / electrostatic field orbitrap high-resolution mass spectrometry detection method for a four-season antiviral mixture, which adopts ultra-high performance liquid chromatography-quadrupole rod / electrostatic field orbitrap high-resolution mass spectrometry, and the optimal chromatographic conditions are as follows: 0-1.0 min, 98%-98% A and 2.0%-2.0% B; 1.0-14.0 min, 98%-70% of A and 2.0%-30.0% of B are added; in 14.0-25.0 min, 70%-0% of A is adopted, and 30%-100% of B is adopted; 25.0-28.0 min, 0%-0% of A and 100%-100% of B are included; from 28.0 min to 28.1 min, 0%-98% of A is adopted, and 100%-2% of B is adopted; 28.1-30.0 min, 98%-98% of A and 2%-2% of B are included; 148 chemical components, including 31 glycosides, 14 terpenoids, 54 flavonoids, 10 lignans, 23 organic acids, 3 alkaloids and 13 other types, are identified in total. The detection method can provide reference for internal quality detection of the traditional Chinese medicine, research on basic components of pharmacodynamic substances of the traditional Chinese medicine and authenticity identification of traditional Chinese medicine components.

The invention belongs to the technical field of medicinal material screening, and relates to a traditional Chinese medicine compound for treating attention deficit hyperactivity disorder and a screening method thereof. The screening method comprises the following steps: constructing a drug use database of ADHD; performing statistics and association rule analysis on frequency, property and flavor tropism and efficacy of the traditional Chinese medicines to obtain a high-frequency traditional Chinese medicine composition; screening ADHD disease targets and establishing a target set; performing clustering analysis and integration on the high-frequency traditional Chinese medicine composition to obtain a core medicine composition; constructing candidate traditional Chinese medicine compounds through reverse network pharmacological analysis; evaluating the efficacy of the candidate traditional Chinese medicine compound through animal experiments, and screening out the optimal traditional Chinese medicine compound. According to the method, an O-R-S analysis mode is constructed through data mining and reverse network pharmacology, traditional Chinese medicine compound screening and evaluation processes of ADHD and candidate compounds with better comprehensive performance are optimized, and a reference scheme with data support and mechanism basis is provided for traditional Chinese medicine clinical treatment and new drug research and development of ADHD.

The present application relates to the technical field of biological medicine, and more particularly to the application of isodon japonicus in the preparation of a drug for preventing and treating spontaneous abortion. The present application analyzes the in-vivo migration components of Jianwei Shoutai Pills, excavates the pharmacodynamic material basis for preventing and treating spontaneous abortion, and uses a CBA / J x DBA / 2 peri-implantation period abortion mouse model to conduct pharmacodynamic experiments. The results show that isodon japonicus can significantly reduce the abortion rate of the CBA / J x DBA / 2 peri-implantation period abortion model mice, and can effectively increase the embryo and placenta wet weight of the CBA / J x DBA / 2 peri-implantation period abortion model mice. That is, isodon japonicus can effectively inhibit spontaneous abortion, has a significant curative effect, has considerable treatment potential, provides a potential new drug selection for clinical drugs for preventing and treating spontaneous abortion, and has a broad application prospect.

The invention discloses a method for analyzing a pharmacodynamic material basis and metabolites of Longqing capsules. According to the method, an UPLC-Q-Exactive-Orbitrap MS technology is adopted, under the same chromatographic condition and mass spectrum condition, the pharmacodynamic material basis and metabolites of the Longqing capsules are measured and analyzed, 128 compounds are analyzed from the Longqing capsules, 144 exogenous compounds including 61 prototype components and 83 metabolites are analyzed from plasma, urine and excrement of rats in total, and the pharmacodynamic material basis and the metabolites of the Longqing capsules are analyzed to form the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules with the pharmacodynamic material basis and the metabolites of the Longqing capsules. The main metabolic pathways comprise oxidation, reduction, hydrolysis, methylation, sulfation, glucuronization, composite reactions of various reaction types and the like. The invention provides a scientific basis for quality control and clinical reasonable medication of Longqing capsule medicines.

The application discloses a kind of material evaluation methods, pharmaceutical compositions and application of sophora fruit products.It is specific, disclose a kind of material evaluation methods of sophora fruit products, it includes the following steps: S1, using liquid chromatography, the fingerprint of the sophora fruit product of different processing time is established, and common characteristic peak is extracted;S2, the hemostatic effect of sophora fruit product of different processing time is determined;S3, according to the characteristic peak data of the sophora fruit product of different processing time and its hemostatic effect data, statistical analysis is carried out by chemometrics method, and the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect

The present invention belongs to the technical field of organic chemical synthesis, and specifically relates to a rhein nitroxide derivative, a synthesis method, and an application thereof. The present invention uses rhein, an antioxidant pharmacological substance from the traditional Chinese medicine rhubarb, as a research object, selects its carboxyl group at position 3 as a modification site, and introduces four active intermediate nitroxides into the rhein structure based on the amide condensation and esterification reaction mechanisms and the pharmacophore splicing principle. Thirty rhein nitroxide derivatives were designed and synthesized, thereby establishing a method for synthesizing novel rhein nitroxide derivatives with mild reaction conditions, environmental friendliness, and high yield. The provided novel rhein nitroxide derivatives have the ability to scavenge DPPH and ABTS free radicals, have a protective effect on oxidatively damaged cells, and have the ability to delay the lifespan of Caenorhabditis elegans. Their effects are superior to those of rhein, providing a new approach for the development of antioxidant and anti-aging drugs.

This invention belongs to the field of modern analytical technology of traditional Chinese medicine, specifically involving a method for detecting chemical components of bitter vine based on UPLC-Q-TOF-MS / MS. This invention is the first to systematically and rapidly perform qualitative analysis on the chemical components of bitter vine, a Dai medicinal herb, identifying a total of 96 compounds, including saponins, phenylpropanoids, flavonoids, terpenes, and organic acids. This provides a scientific basis and data support for elucidating the pharmacodynamic material basis, quality control, and subsequent formulation development of bitter vine.

The invention relates to the technical field of medicine analysis, and particularly provides a method for establishing a hydrangea serrata medicine characteristic chromatogram as well as the characteristic chromatogram and application of the hydrangea serrata medicine characteristic chromatogram. The method not only solves the problem of disordered base sources of the sparassis crispa and clematis armandii decoction pieces and standard decoction, but also solves the problems of low extraction rate of effective components and difficulty in detection of formula granules and standard decoction conventional methods. The establishment method of the drug characteristic spectrum of the sparassis crispa can be used for quality control of the sparassis crispa formula granules, common control of active ingredients such as caffeic acid and ferulic acid can be achieved at the same time, basic sources of the sparassis crispa and standard decoction are effectively identified, the content of ferulic acid is limited, and overall quality control of the sparassis crispa formula granules is achieved. The application of the preparation method of the sparassis crispa formula granules and decoction piece screening in practice can effectively ensure that pharmacodynamic substances between the sparassis crispa formula granules and a standard decoction are consistent with the standard decoction, so that the quality of the prepared sparassis crispa formula granules is higher and more stable.

This invention provides a method for screening the active ingredient groups of Xinsu Ning capsules, relating to the technical field of quality control and pharmacodynamic substance analysis of traditional Chinese medicine. The screening method for the active ingredient groups of Xinsu Ning capsules provided by this invention integrates non-targeted chemical component analysis, in vivo prototype and metabolite detection, network pharmacology correlation analysis, and fingerprint spectrum construction, enabling a systematic evaluation of the in vivo exposure characteristics, target regulatory potential, and quality consistency of candidate components. This method can identify chemical components related to pharmacodynamic effects from complex systems, providing an operable technical path for the quality control and pharmacodynamic substance basis research of Xinsu Ning capsules.