6 results about "Pharmacological Substance" patented technology

This invention belongs to the field of modern analytical technology of traditional Chinese medicine, specifically involving a method for detecting chemical components of bitter vine based on UPLC-Q-TOF-MS / MS. This invention is the first to systematically and rapidly perform qualitative analysis on the chemical components of bitter vine, a Dai medicinal herb, identifying a total of 96 compounds, including saponins, phenylpropanoids, flavonoids, terpenes, and organic acids. This provides a scientific basis and data support for elucidating the pharmacodynamic material basis, quality control, and subsequent formulation development of bitter vine.

This invention provides a method for screening the active ingredient groups of Xinsu Ning capsules, relating to the technical field of quality control and pharmacodynamic substance analysis of traditional Chinese medicine. The screening method for the active ingredient groups of Xinsu Ning capsules provided by this invention integrates non-targeted chemical component analysis, in vivo prototype and metabolite detection, network pharmacology correlation analysis, and fingerprint spectrum construction, enabling a systematic evaluation of the in vivo exposure characteristics, target regulatory potential, and quality consistency of candidate components. This method can identify chemical components related to pharmacodynamic effects from complex systems, providing an operable technical path for the quality control and pharmacodynamic substance basis research of Xinsu Ning capsules.

This invention provides a method for simultaneously determining the content of 32 components in Xinsu Ning capsules based on UHPLC-MS / MS and its application, relating to the technical field of traditional Chinese medicine quality testing. The method for simultaneously determining the content of 32 components in Xinsu Ning capsules based on UHPLC-MS / MS includes: preparing a test solution from a Xinsu Ning capsule sample; detecting at least 30 active ingredients in the test solution using liquid chromatography-tandem mass spectrometry under positive and / or negative ion ionization modes; and calculating the content of each component using a standard curve. This invention achieves simultaneous quantification of 32 key components in Xinsu Ning capsules (covering multiple categories such as alkaloids, flavonoids, triterpenoid saponins, and coumarins), comprehensively covering the characteristic active ingredients of each medicinal material in the prescription, avoiding quality evaluation bias caused by incomplete component coverage; and providing data support for the correlation between the quality and efficacy of Xinsu Ning capsules through simultaneous quantification of multiple components, thus contributing to its quality consistency evaluation and pharmacodynamic material basis research.

The application discloses a two-dimensional chromatography screening method of lipid-lowering active ingredients of Linderae Radix, and comprises the following steps: extraction of Linderae Radix terpenoid enrichment, establishment of a two-dimensional HSCCCxUHPLC-MS chromatogram, establishment of a two-dimensional bioactivity atlas and identification of the lipid-lowering active ingredients of Linderae Radix; the method integrates the advantages of two chromatography technologies, can quickly and accurately screen the effective active ingredients in the Linderae Radix terpenoid enrichment, and is used for screening acetyl linderae resinoid from the Linderae Radix terpenoid enrichment; the acetyl linderae resinoid has a lipid-lowering activity, which is reported for the first time; and the application provides a new basis for clarifying the lipid-lowering pharmacodynamic material basis of Linderae Radix and related drug development.

This invention belongs to the field of phytochemistry and pharmaceutical technology, specifically disclosing a method for preparing and purifying sesquiterpene coumarin compounds isolated from *Ferula samarkand*, including the structures of three sesquiterpene coumarin compounds isolated from *Ferula samarkand*, and the process for separating sesquiterpene coumarins. Addressing the current problem of unclear chemical composition and active ingredients of *Ferula samarkand*, this invention employs organic solvent extraction, concentration, and then utilizes two to three methods—normal-phase silica gel column chromatography, dextran gel chromatography, low-to-medium pressure rapid preparative chromatography, and semi-preparative high-performance liquid chromatography—for separation, ultimately yielding three sesquiterpene coumarin monomers. This solves the problem in existing technologies where the unclear chemical composition and active ingredients of *Ferula samarkand* make it difficult to separate and purify sesquiterpene coumarins.

This invention relates to the field of biomedical technology, and particularly to a method and application for identifying active ingredients for the prevention and treatment of diabetes from non-medicinal Astragalus membranaceus slices. The method includes: first, qualitative and quantitative analysis of the chemical components of Astragalus membranaceus and longan slices; second, evaluation of its hypoglycemic efficacy using a diabetic rat model to verify the effects of Astragalus membranaceus and longan slices in lowering blood sugar, improving insulin resistance, and reducing inflammation; and finally, integration of non-targeted and pseudo-targeted metabolomics technologies to systematically identify the core active ingredients in key pathways for the prevention and treatment of diabetes by analyzing the correlation between chemical components and efficacy indicators. This invention not only reveals the main pharmacodynamic substances of Astragalus membranaceus in the prevention and treatment of diabetes, but also provides a theoretical basis and technical support for the development of related functional products or innovative drugs, and for comprehensively improving the utilization efficiency and economic value of Astragalus membranaceus resources.