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Application of trollius chinensis bunge extract in preparation of drugs for treating virus diseases

A technology of nasturtium lotus extract and viral diseases, applied in the field of medicine, can solve the problems of large toxic and side effects, increase of opportunistic pathogens, etc., and achieve the effect of small dosage

Active Publication Date: 2014-03-12
CHENGDE TIANYUAN PHARMACEUTICAL INDUSTRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the widespread use of chemically synthesized and semi-synthesized antibacterial and antiviral drugs for more than half a century, drug-resistant strains of bacteria have been induced, and the number of opportunistic pathogenic bacteria is increasing day by day.
Coupled with its disadvantages such as relatively large toxic and side effects, although this type of drug is used as a first-line treatment drug, there are many shortcomings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Detection of Inhibitory Activity of Trollius Flower Extract against Influenza Virus (H1N1) in Vitro

[0012] Materials and Methods

[0013] 1. In vitro host cells: MDCK (dog kidney passage cells) cells.

[0014] 2. Virus strain: Influenza virus type A (H1N1) (A3 / Jingke / 30 / 95).

[0015] 3. CPE method to detect the toxicity of drugs to cells.

[0016] 4. CPE method (cytopathic) to detect the inhibitory effect of drugs on influenza virus.

[0017] Experimental procedure

[0018] (1). Cytotoxicity detection of drugs

[0019] After MDCK cells were cultured in a 96-well cell culture plate to form a monolayer, different concentrations of drug solutions were added, and the culture was continued for 3 days. The toxicity of drugs to MDCK cells was detected by CPE method.

[0020] (2). Detection of drug anti-influenza virus effect

[0021] After the MDCK cells were cultured in a 96-well cell culture plate to form a monolayer, the cells were infected with 100 TCI...

Embodiment 2

[0024] Example 2: Detection of Inhibitory Activity of Trollius Flower Extract on Respiratory Syncytial Virus (RSV) in Vitro

[0025] Materials and Methods

[0026] 1. In vitro host cells: Vero (African green monkey kidney cells) cells.

[0027] 2. Virus strain: respiratory syncytial virus (RSV).

[0028] 3. CPE method to detect the toxicity of drugs to cells.

[0029] 4. CPE (cytopathic) method to detect the inhibitory effect of drugs on respiratory syncytial virus.

[0030] Experimental procedure

[0031] (1). Cytotoxicity detection of drugs

[0032] After Vero cells were cultured in a 96-well cell culture plate to form a monolayer, drug solutions of different concentrations were added and cultured for 3 days, and the toxicity of drugs to Vero cells was detected by CPE method.

[0033] (2). Detection of anti-respiratory syncytial virus effect of drugs

[0034] After Vero cells were cultured in a 96-well cell culture plate to form a monolayer, the cells were infected w...

Embodiment 3

[0037] Example 3: Detection of the Inhibitory Activity of Trollius Flower Extract against Adenovirus (Adv3) in Vitro

[0038] Materials and Methods

[0039] 1. In vitro host cells: Vero (African green monkey kidney cells) cells.

[0040] 2. Virus strain: adenovirus (Adv 3 ).

[0041] 3. CPE method to detect the toxicity of drugs to cells.

[0042] 4. CPE method (cytopathic) to detect the inhibitory effect of drugs on adenovirus.

[0043] Experimental procedure

[0044] (1). Cytotoxicity detection of drugs

[0045] After Vero cells were cultured in a 96-well cell culture plate to form a monolayer, drug solutions of different concentrations were added and cultured for 3 days, and the toxicity of drugs to Vero cells was detected by CPE method.

[0046] (2). Drug anti-adenovirus detection

[0047] After Vero cells were cultured in a 96-well cell culture plate to form a monolayer, 100 TCID50 of adenovirus (Adv 3 ) to infect the cells, and then add a series of drug-contain...

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PUM

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Abstract

The invention discloses application of a trollius chinensis bunge extract in preparation of drugs for treating virus diseases. The purity of the trollius chinensis bunge extract is as follows: the total flavonoid content of the trollius chinensis bunge extract is higher than 50%. The trollius chinensis bunge extract is extracted from plants, traditional Chinese medicinal materials and Chinese herbal pieces all containing the trollius chinensis bunge. The trollius chinensis bunge extract can be applied to preparation of drugs for treating influenza virus H1N1, drugs for treating respiratory syncytial virus (RSV), and drugs for inhibiting the activity of and treating adenovirus type 3 (Adv3). The trollius chinensis bunge extract can be mixed with a barrier or an excipient acceptable on medicine to prepare the clinically acceptable medicinal preparation. According to the invention, the efficacy material for treating virus diseases from the traditional Chinese medicine, namely the trollius chinensis bunge extract, is found out, so that the scientific basis is provided for the clinical application of the extract, and the trollius chinensis bunge extract can be used as a natural antiviral agent, and has the characteristics of no addiction and smallness in dosage.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to the new application of the extract of Trollius chinensis in the field of pharmacy, in particular to the application of the extract of Trollius chinensis in the preparation of medicines for treating viral diseases. Background technique [0002] From the rampant "SARS virus" in 2003, to the fierce attack of "H1N1 influenza" in 2009, and to the lingering power of "H7N9 bird flu" this year, in just ten years, we have repeatedly and repeatedly Attacked by a virus. Although historical records tell us that viruses, as a species, have existed far longer than humans, and in the history of human development, they have posed far more threats to human survival than other species. [0003] Viral infectious diseases caused by various respiratory viruses are a group of common diseases, the incidence of which is gradually increasing in the world, and new pathogenic viruses are constantly emer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P31/12A61P31/16A61P31/14A61P31/20A61K133/00
Inventor 张国刚
Owner CHENGDE TIANYUAN PHARMACEUTICAL INDUSTRY CO LTD
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