2results about How to "Immunomodulatory" patented technology

The invention provides sequoia sempervirens stem fermentation liquor and a preparation method and application thereof, and the preparation method specifically comprises the following steps: (1) crushing dried bark of sequoia sempervirens, adding inulin, a carbon source, a nitrogen source, inorganic salt and water, and sterilizing to obtain a dried bark culture medium of sequoia sempervirens; and (2) inoculating saccharomyces cerevisiae and bacillus subtilis into the sequoia sempervirens dry bark culture medium for fermentation, and then sterilizing and centrifuging to obtain sequoia sempervirens stem fermentation liquor. Experiments show that the sequoia sempervirens stem fermentation liquor prepared by the method provided by the invention is high in active ingredient content, and has various effects of resisting inflammation, relieving and resisting allergy, promoting wound healing, resisting aging and the like. The fermentation liquor prepared by the method provided by the invention can be widely applied to preparation of cosmetics with anti-aging, anti-allergy and repairing effects.

This invention relates to the field of biomedical technology and provides a controllable cryogenic treatment mesenchymal stem cell preparation, its preparation method, and its application. The method includes the following steps: cell preparation and cryopreservation equilibration: digestion and collection; cryopreservation equilibration; removal of cryoprotectant and washing: centrifugation to remove cryopreservation solution; PBS washing; cryogenic incubation: resuspending the final cell pellet after multiple washes in pre-cooled PBS; incubating the cell suspension at 4±0.5℃ for 72 hours±10 minutes; after incubation, removing the cell suspension from the 4℃ environment, centrifuging, and resuspending in PBS. This invention yields a ready-to-use, easily stored and transported, and functionally enhanced RT-MSCs preparation. Although the cells in this preparation have lost their proliferative and metabolic activity, they still retain the complete therapeutic functions of MSCs, such as immunomodulation, anti-fibrosis, and angiogenesis promotion.