Anti-allergy soothing sanitary napkin composite chip containing purslane component and preparation method of anti-allergy soothing sanitary napkin composite chip

Abstract

The invention provides an anti-allergy soothing sanitary towel composite chip containing a purslane component and a preparation method, and belongs to the technical field of sanitary towels. The composite chip is of a multi-layer structure, the middle layer is a water absorption layer, the upper layer is an anti-allergy soothing layer, and the lower layer is a water-resisting layer; the anti-allergy soothing layer is a nanofiber membrane loaded with probiotic microspheres and a purslane extract, and the water-resisting layer is a polyethylene film or a polypropylene film; the water absorption layer is a composite layer of silicon dioxide aerogel and polyurethane sponge. The prepared anti-allergy soothing sanitary towel composite chip containing the purslane component not only has various biological functions of absorption, deodorization, peculiar smell removal, antibiosis, anti-inflammation, anti-allergy soothing, antioxidation, adjustment of ecological balance of private parts and the like, so that the effects of relieving itching and allergy, protecting skin barriers, resisting inflammation and the like are achieved, vaginal flora can be adjusted, and the effects of relieving itching, relieving itching, relieving inflammation and the like are achieved. And vaginitis and the like are reduced, the private health of women in the menstrual period is protected, and the health of women is facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of sanitary napkins, and in particular to an anti-allergic and soothing sanitary napkin composite chip containing purslane ingredients and a preparation method thereof. Background Art

[0002] Sanitary napkins are sanitary products used by women during menstruation. The main materials are cotton, non-woven fabrics, pulp or polymers and polymer composite paper formed by the above materials. The side design is mainly used to prevent side leakage. From the inside to the outside, it is divided into three layers: surface layer, absorbent core, and bottom layer. When using, tear off the paper tape in the middle first, stick the adhesive side to the middle of the underwear first, and stick the two "ears" to the narrowest part of the crotch. Other common menstrual products include tampons, menstrual cups, panty liners, etc. With the development of science and technology and the gradual improvement of people's quality of life, the types of sanitary napkins are gradually enriched.

[0003] For example, in the patent with publication number CN116492145A, a sanitary napkin with antibacterial and deodorizing properties and its preparation process are disclosed. In the sanitary napkin, viscose fibers and bamboo fibers of different finenesses are used to design a double-layer composite spunlace non-woven fabric, so as to enable the liquid to move from the viscose fiber side to the bamboo fiber side, thereby achieving unidirectional moisture conduction, preventing backseepage, and having excellent antibacterial properties.

[0004] For example, the patent with announcement number CN115414185B discloses a highly dry sanitary napkin and its production process, which includes a surface layer, a menthol sustained-release agent layer, an absorbent core layer and a bottom film from top to bottom, wherein the surface layer is a unidirectional water-permeable spinning film, which is made of a polyurethane fiber film and a pure cotton non-woven fabric through an electrostatic spinning process, and the polyurethane fiber film is located on the pure cotton non-woven fabric. The sanitary napkin has strong water absorption, high air permeability, a dry surface, and is comfortable to touch the skin without irritation or dampness.

[0005] From the above examples, we can also find that the existing improvements on sanitary napkins are mainly focused on improving the water absorption capacity of sanitary napkins and keeping the surface dry. However, in actual use, it can be found that in addition to menstrual blood, there are also blood clots and endometrial tissue discharged during the physiological period. Therefore, even if the sanitary napkin has good water absorption performance, these blocky substances will still remain on the surface of the sanitary napkin, causing bacterial growth, causing skin itching, vaginitis and other problems. Some sanitary napkin materials are even prone to skin allergic reactions, causing skin itching and other problems.

[0006] Portulaca oleracea L., also known as ant vegetable, is an annual fleshy herb of the genus Portulaca in the family Portulacaceae. It is widely distributed in temperate and tropical regions around the world and is distributed throughout my country. Portulaca oleracea is a plant used for both medicinal and edible purposes, and mainly contains active substances such as alkaloids, terpenes, coumarins, flavonoids, organic acids, volatile oils and polysaccharides. According to the 27th volume of Compendium of Materia Medica, Portulaca oleracea can be used clinically to "disperse blood and reduce swelling, facilitate bowel movements, detoxify and relieve stranguria, and treat postpartum sweating". Studies have shown that Portulaca oleracea alkaloids have anti-inflammatory, analgesic, antibacterial, blood sugar and blood lipid regulation, anti-tumor, antioxidant, anti-aging, and immune enhancement effects. Summary of the invention

[0007] The purpose of the present invention is to propose an anti-allergic and soothing sanitary napkin composite chip containing purslane ingredients and a preparation method, which not only has multiple biological functions such as absorption, deodorization and odor removal, antibacterial and anti-inflammatory, anti-allergic and soothing, antioxidant, and regulation of private ecological balance, thereby improving itching and allergies, protecting the skin barrier, and anti-inflammatory effects, but also can regulate vaginal flora, reduce the occurrence of vaginitis, etc., protect the private health of women during menstruation, and is more beneficial to women's health.

[0008] The technical solution of the present invention is achieved in this way:

[0009] The present invention provides an anti-allergic and soothing sanitary napkin composite chip containing purslane ingredients. The composite chip is a multi-layer structure, wherein the middle layer is a water-absorbing layer, the upper layer is an anti-allergic and soothing layer, and the lower layer is a water-isolating layer; the anti-allergic and soothing layer is a nanofiber membrane loaded with probiotic microspheres and purslane extract, the water-isolating layer is a polyethylene film or a polypropylene film; the water-absorbing layer is a composite layer of silica aerogel and polyurethane sponge.

[0010] As a further improvement of the present invention, the preparation method of the nanofiber membrane loaded with probiotic microspheres and purslane extract is as follows:

[0011] S1. Enzyme-assisted fermentation of purslane: Wash, dry, and chop purslane, add to water, add sad enzyme and cellulase, stir for enzymolysis, add Lactobacillus plantarum and Bifidobacterium lactis seed solution, enzyme-assisted fermentation culture, filter, wash the solid, collect the bacterial solution, and retain the solid residue; freeze-dry the filtrate to obtain an extract;

[0012] S2. Aqueous two-phase extraction: adding the solid residue to an aqueous solution of ethanol and ammonium sulfate, performing ultrasonic-assisted heating extraction, filtering, dialyzing the filtrate, and drying to obtain an alkaloid extract;

[0013] S3. Macroporous resin purification: The extract was purified by macroporous resin to obtain a flavonoid extract;

[0014] S4. Preparation of probiotic microspheres: adding sodium alginate and Tween to the bacterial solution, adding dropwise to the fish oil, emulsifying, adding dropwise calcium chloride solution, solidifying at room temperature, centrifuging, washing, and drying to obtain probiotic microspheres;

[0015] S5. Preparation of modified nanofiber membrane: dissolve polyacrylonitrile in dimethylformamide, add alkaloid extract and flavonoid extract, stir and mix evenly to obtain spinning solution, use electrospinning machine to spin to obtain nanofiber membrane, then add probiotic microspheres into water, spray evenly on the surface of nanofiber membrane to obtain modified nanofiber membrane.

[0016] As a further improvement of the present invention, the mass ratio of purslane, enzyme and cellulase in step S1 is 100:1-2:2-4, the temperature of the enzymolysis is 35-45°C, the time is 1-2h, and the bacterial content of the plant lactobacillus and bifidobacterium lactis seed solution is 10 8 -10 9 cfu / mL, the conditions of the enzyme-assisted fermentation culture are 36-38°C, 100-200r / min, and anaerobic conditions, and the enzyme-assisted fermentation culture is 24-36h, and the bacterial liquid has a bacterial content of 10 9 -10 10 cfu / mL.

[0017] As a further improvement of the present invention, the solid-to-liquid ratio of the solid residue, ethanol and the aqueous solution of ammonium sulfate in step S2 is 1:5-10 g / mL, the ethanol content in the aqueous solution of ethanol and ammonium sulfate is 10-20wt%, the ammonium sulfate content is 12-15wt%, the ultrasonic power of the ultrasonic-assisted heating extraction is 1000-1500W, the heating temperature is 50-60°C, and the time is 2-4h.

[0018] As a further improvement of the present invention, the macroporous resin in step S3 is selected from at least one of D-101, AB-8, and DA-201, and the eluent used for purification is a 45-65wt% ethanol solution.

[0019] As a further improvement of the present invention, the mass ratio of the bacterial solution, sodium alginate and Tween in step S4 is 100:12-15:1-2, and the Tween is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80 and Tween-85.

[0020] As a further improvement of the present invention, the mass ratio of polyacrylonitrile, alkaloid extract, flavonoid extract and probiotic microspheres in step S5 is 100:12-15:10-14:5-7, and the spinning conditions are: the syringe needle is 21G, the spinning solution push speed is 0.5-1mL / h, the spinning voltage is 15-20kV, the receiving distance is 10-20cm, and the receiving roller speed is 400-800r / min.

[0021] As a further improvement of the present invention, the preparation method of the composite layer of the silica aerogel and the polyurethane sponge is as follows: adding the silica aerogel to a Tris-HCl solution, adding dopamine hydrochloride, heating and stirring to react, filtering, washing, and drying to obtain a modified silica aerogel, and uniformly spraying it on the surface of the polyurethane sponge to obtain a composite layer.

[0022] As a further improvement of the present invention, the pH value of the Tris-HCl solution is 8.5-9.5, the mass ratio of the silica aerogel to dopamine hydrochloride is 10:3-4, the temperature of the heating and stirring reaction is 40-50°C, the time is 2-3h, and the spraying amount of the modified silica aerogel is 0.5-1g / cm 2 .

[0023] The present invention further protects a method for preparing the above-mentioned sanitary napkin composite chip, comprising the following steps: stacking a waterproof layer, a water-absorbing layer, and an anti-allergic soothing layer in sequence from bottom to top, and then compacting for 5-10 minutes at a pressure of 10-15Mpa and a temperature of 80-90°C to obtain a sanitary napkin composite chip.

[0024] The present invention has the following beneficial effects:

[0025] The present invention prepares an anti-allergic and soothing sanitary napkin composite chip containing purslane ingredients. The composite chip is a multi-layer structure, wherein the middle layer is a water-absorbing layer, the upper layer is an anti-allergic and soothing layer, and the lower layer is a water-isolating layer; the water-isolating layer can well isolate wet objects and prevent them from penetrating into clothes; the middle layer is a water-absorbing layer, which can absorb most of the moisture in wet objects and has a high water absorption capacity; the composite chip is a composite layer of silica aerogel and polyurethane sponge; the polyurethane sponge has a good water absorption effect; the silica aerogel has a porous structure, which can not only improve the water absorption capacity, but also have the effects of deodorization and odor removal.

[0026] The anti-allergic and soothing layer is a nanofiber membrane loaded with probiotic microspheres and purslane extract. Purslane extract is rich in active substances, such as polysaccharides, alkaloids, flavonoids, terpenes, organic acids, etc., which have multiple biological functions such as antibacterial and anti-inflammatory, anti-allergic and soothing, antioxidant, and regulation of private area ecological balance, thereby improving itching and allergies, protecting the skin barrier, and anti-inflammatory effects.

[0027] The present invention uses laccase and cellulase to perform preliminary enzymolysis on purslane. Laccase is a kind of oxidoreductase, which is composed of protein and Cu 2+ It can catalyze more than 250 substrates, including phenols and their derivatives, aromatic amines and their derivatives, carboxylic acids and their derivatives, steroid hormones and biological pigments, etc. Cellulase can destroy plant cell walls and promote the dissolution of plant cell contents. In the process of enzyme-assisted fermentation, it further promotes the dissolution of active components in Purslane plant cells, and promotes the synthesis of more active components including antibacterial flavonoids, anti-inflammatory terpenes, etc., greatly improving the generation of active products.

[0028] Compared with the traditional extraction method, the two-phase aqueous extraction has the characteristics of short phase separation time, easy continuous operation, large target product distribution coefficient, low investment cost, etc. The present invention uses two-phase aqueous extraction to extract the solid residue, which can be well enriched in one phase, greatly improving the extraction rate of alkaloids. The present invention uses macroporous resin purification to obtain highly active flavonoid components, thereby obtaining flavonoid extracts, which have antibacterial and anti-inflammatory, anti-allergic and soothing, antioxidant, and immunomodulatory effects.

[0029] Polyacrylonitrile is a polymer with high thermal stability and strong spinnability. It is an excellent material for preparing electrostatic spinning nanofiber membranes. However, it has no antibacterial and antioxidant properties, and is easily prone to the accumulation of harmful bacteria and free radicals in the private parts, triggering inflammatory reactions, causing eczema or allergic symptoms. The present invention greatly improves the material's multiple biological functions such as antibacterial and anti-inflammatory, anti-allergic and soothing, anti-oxidation, and regulating the ecological balance of the private parts by adding purslane extract, thereby improving itching, allergic conditions, protecting the skin barrier, and anti-inflammatory effects. In addition, embedded probiotics are also sprayed, which improves the survival rate of probiotics and improves its stability on the one hand. On the other hand, the probiotic microspheres break when used, and the probiotics are released and enter the vagina, regulating the vaginal flora, reducing the occurrence of odor and vaginitis, protecting the private health of women during the physiological period, and being more conducive to the health of women.

[0030] The anti-allergic and soothing sanitary napkin composite chip containing purslane ingredients prepared by the present invention not only has multiple biological functions such as absorption, deodorization and odor removal, antibacterial and anti-inflammatory, anti-allergic and soothing, anti-oxidation, and regulation of private ecological balance, thereby improving itching and allergies, protecting the skin barrier, and anti-inflammatory effects, but can also regulate vaginal flora, reduce the occurrence of vaginitis, etc., protect the private health of women during the menstrual period, and is more beneficial to women's health. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Lactobacillus plantarum, 10 billion cfu / g, Bifidobacterium lactis, 10 billion cfu / g, were purchased from Shandong Zhongke Jiayi Bioengineering Technology Co., Ltd. Preparation of Lactobacillus plantarum and Bifidobacterium lactis seed solution: The strains were inoculated into Gao's medium, activated and cultured at 37°C, 100 r / min for 18-24 h, and a culture medium with a bacterial content of 10 8 -10 9 cfu / mL of bacterial seed solution.

[0033] Cellulase, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.; cellulase, 10,000 U / g, purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd. Silica aerogel, powder, density <200 kg / m 3 , purchased from Langmiao Environmental Technology Tianjin Co., Ltd.

[0034] Preparation Example 1-1 Composite layer of silica aerogel and polyurethane sponge

[0035] The preparation method is as follows: add 10g of silica aerogel to a Tris-HCl solution with a pH value of 8.5, add 3g of dopamine hydrochloride, heat to 40°C, stir and react for 2h, filter, wash, and dry to obtain modified silica aerogel, which is evenly sprayed on the surface of the polyurethane sponge at a spraying amount of 0.5g / cm 2 , a composite layer is obtained.

[0036] Preparation Example 1-2 Composite layer of silica aerogel and polyurethane sponge

[0037] The preparation method is as follows: add 10g of silica aerogel to a Tris-HCl solution with a pH value of 9.5, add 4g of dopamine hydrochloride, heat to 50°C, stir and react for 3h, filter, wash, and dry to obtain modified silica aerogel, which is evenly sprayed on the surface of the polyurethane sponge at a spraying amount of 1g / cm 2 , a composite layer is obtained.

[0038] Preparation Example 1-3 Composite layer of silica aerogel and polyurethane sponge

[0039] The preparation method is as follows: 10 g of silica aerogel is added to a Tris-HCl solution with a pH value of 9, 3.5 g of dopamine hydrochloride is added, the solution is heated to 45 °C, stirred for reaction for 2.5 h, filtered, washed, and dried to obtain the modified silica aerogel, which is evenly sprayed on the surface of the polyurethane sponge at a spraying amount of 0.7 g / cm 2 , a composite layer is obtained.

[0040] Comparative Preparation Example 1

[0041] Compared with Preparation Example 1-3, the difference is that the composite layer of silica aerogel and polyurethane sponge is replaced by polyurethane sponge.

[0042] Preparation Example 2-1 Nanofiber membrane loaded with probiotic microspheres and purslane extract

[0043] The preparation method is as follows:

[0044] S1. Enzyme-assisted fermentation of purslane: 10 g purslane was washed, dried, chopped, added to 500 mL of water, 0.1 g of enzyme and 0.2 g of cellulase were added, stirred, heated to 35 ° C, enzymatic hydrolysis for 1 h, and seed liquid of Lactobacillus plantarum and Bifidobacterium lactis were added, the inoculation amount was 1 v / v% and 1.5 v / v%, respectively, at 36 ° C, 100 r / min, under anaerobic conditions, enzyme-assisted fermentation was cultured for 24 h, filtered, solid washed, and the bacterial liquid was collected, and the bacterial content was 10 9 -10 10 cfu / mL, the solid residue was retained; the filtrate was freeze-dried to obtain the extract;

[0045] S2. Aqueous two-phase extraction: 10 g of the solid residue was added to 50 mL of an aqueous solution of ethanol and ammonium sulfate, wherein the ethanol content of the aqueous solution of ethanol and ammonium sulfate was 10 wt %, and the ammonium sulfate content was 12 wt %, and 1000 W ultrasonic-assisted heating was performed to 50 ° C. The mixture was stirred and extracted for 2 h, filtered, the filtrate was dialyzed, and dried to obtain an alkaloid extract;

[0046] S3 macroporous resin purification: The extract was purified with a macroporous resin AB-8, and the eluate of 45-65wt% ethanol solution was collected and dried to obtain a flavonoid extract;

[0047] S4. Preparation of probiotic microspheres: 12 g of sodium alginate and 1 g of Tween-60 were added to 100 g of bacterial solution, added dropwise to 500 mL of fish oil, emulsified at 8000 r / min for 15 min, 10 mL of 2 wt % calcium chloride solution was added dropwise, solidified at room temperature for 30 min, centrifuged, washed, and dried to obtain probiotic microspheres;

[0048] S5. Preparation of modified nanofiber membrane: 10 g of polyacrylonitrile was dissolved in 500 mL of dimethylformamide, 1.2 g of alkaloid extract and 1 g of flavonoid extract were added, and the mixture was stirred for 30 min to obtain a spinning solution, which was spun using an electrospinning machine to obtain a nanofiber membrane, and then 0.5 g of probiotic microspheres were added to 50 mL of water and evenly sprayed on the surface of the nanofiber membrane to obtain a modified nanofiber membrane;

[0049] The spinning conditions are as follows: the syringe needle is 21G, the spinning solution pushing speed is 0.5 mL / h, the spinning voltage is 15 kV, the receiving distance is 10 cm, and the receiving roller speed is 400 r / min.

[0050] Preparation Example 2-2 Nanofiber membrane loaded with probiotic microspheres and purslane extract

[0051] The preparation method is as follows:

[0052] S1. Enzyme-assisted fermentation of purslane: 10 g purslane was washed, dried, chopped, added to 500 mL of water, 0.2 g of enzyme and 0.4 g of cellulase were added, stirred, heated to 45 ° C, enzymatic hydrolysis for 2 h, and seed liquid of Lactobacillus plantarum and Bifidobacterium lactis were added, the inoculation amount was 2 v / v% and 2.5 v / v%, respectively, at 38 ° C, 200 r / min, under anaerobic conditions, enzyme-assisted fermentation was cultured for 36 h, filtered, solid washed, and the bacterial liquid was collected. The bacterial count was 10 9 -10 10 cfu / mL, the solid residue was retained; the filtrate was freeze-dried to obtain the extract;

[0053] S2. Aqueous two-phase extraction: 10 g of the solid residue was added to 100 mL of an aqueous solution of ethanol and ammonium sulfate, wherein the ethanol content of the aqueous solution of ethanol and ammonium sulfate was 20 wt %, and the ammonium sulfate content was 15 wt %, and 1500 W ultrasonic-assisted heating was performed to 60 ° C. The mixture was stirred and extracted for 4 h, filtered, and the filtrate was dialyzed and dried to obtain an alkaloid extract;

[0054] S3 macroporous resin purification: The extract was purified with a macroporous resin AB-8, and the eluate of 45-65wt% ethanol solution was collected and dried to obtain a flavonoid extract;

[0055] S4. Preparation of probiotic microspheres: 15 g of sodium alginate and 2 g of Tween-40 were added to 100 g of bacterial solution, added dropwise to 500 mL of fish oil, emulsified at 8000 r / min for 15 min, 10 mL of 2 wt % calcium chloride solution was added dropwise, cured at room temperature for 30 min, centrifuged, washed, and dried to obtain probiotic microspheres;

[0056] S5. Preparation of modified nanofiber membrane: 10 g of polyacrylonitrile was dissolved in 500 mL of dimethylformamide, 1.5 g of alkaloid extract and 1.4 g of flavonoid extract were added, and the mixture was stirred for 30 min to obtain a spinning solution, which was spun using an electrospinning machine to obtain a nanofiber membrane, and then 0.7 g of probiotic microspheres were added to 50 mL of water and evenly sprayed on the surface of the nanofiber membrane to obtain a modified nanofiber membrane;

[0057] The spinning conditions are as follows: the syringe needle is 21G, the spinning solution pushing speed is 1 mL / h, the spinning voltage is 20 kV, the receiving distance is 20 cm, and the receiving roller speed is 800 r / min.

[0058] Preparation Example 2-3 Nanofiber membrane loaded with probiotic microspheres and purslane extract

[0059] The preparation method is as follows:

[0060] S1. Enzyme-assisted fermentation of purslane: 10 g purslane was washed, dried, chopped, added to 500 mL of water, 0.15 g of enzyme and 0.3 g of cellulase were added, stirred, heated to 40 ° C, enzymatic hydrolysis for 1.5 h, and seed solution of Lactobacillus plantarum and Bifidobacterium lactis were added, the inoculation amount was 1.5 v / v% and 2 v / v%, respectively, at 37 ° C, 150 r / min, under anaerobic conditions, enzyme-assisted fermentation was cultured for 30 h, filtered, solid washed, and the bacterial solution was collected, and the bacterial count was 10 9 -10 10 cfu / mL, the solid residue was retained; the filtrate was freeze-dried to obtain the extract;

[0061] S2. Aqueous two-phase extraction: 10 g of the solid residue was added to 70 mL of an aqueous solution of ethanol and ammonium sulfate, wherein the ethanol content of the aqueous solution of ethanol and ammonium sulfate was 15 wt %, and the ammonium sulfate content was 13 wt %, and 1200 W ultrasonic-assisted heating was performed to 55 ° C. The mixture was stirred and extracted for 3 h, filtered, the filtrate was dialyzed, and dried to obtain an alkaloid extract;

[0062] S3 macroporous resin purification: The extract was purified with a macroporous resin AB-8, and the eluate of 45-65wt% ethanol solution was collected and dried to obtain a flavonoid extract;

[0063] S4. Preparation of probiotic microspheres: 14 g of sodium alginate and 1.5 g of Tween-85 were added to 100 g of bacterial solution, added dropwise to 500 mL of fish oil, emulsified at 8000 r / min for 15 min, 10 mL of 2 wt % calcium chloride solution was added dropwise, cured at room temperature for 30 min, centrifuged, washed, and dried to obtain probiotic microspheres;

[0064] S5. Preparation of modified nanofiber membrane: 10 g of polyacrylonitrile was dissolved in 500 mL of dimethylformamide, 1.4 g of alkaloid extract and 1.2 g of flavonoid extract were added, and the mixture was stirred for 30 min to obtain a spinning solution, which was spun using an electrospinning machine to obtain a nanofiber membrane, and then 0.6 g of probiotic microspheres were added to 50 mL of water and evenly sprayed on the surface of the nanofiber membrane to obtain a modified nanofiber membrane;

[0065] The spinning conditions are as follows: the syringe needle is 21G, the spinning solution pushing speed is 0.7mL / h, the spinning voltage is 17kV, the receiving distance is 15cm, and the receiving roller speed is 600r / min.

[0066] Comparative Preparation Example 2

[0067] Compared with Preparation Example 2-3, the difference is that no enzyme and cellulase are added in step S1.

[0068] The details are as follows:

[0069] S1. Enzyme-assisted fermentation of purslane: 10 g of purslane was washed, dried, chopped, added to 500 mL of water, and seed solution of Lactobacillus plantarum and Bifidobacterium lactis was added, with inoculation amounts of 1.5 v / v% and 2 v / v%, respectively, at 37 ° C, 150 r / min, under anaerobic conditions, fermented for 30 h, filtered, solid washed, and the bacterial solution was collected, with a bacterial count of 10 9 -10 10 cfu / mL, the solid residue was retained; the filtrate was freeze-dried to obtain the extract.

[0070] Comparative Preparation Example 3

[0071] Compared with Preparation Example 2-3, the difference is that fermentation is not performed in step S1.

[0072] The details are as follows:

[0073] S1. Enzyme-assisted fermentation of purslane: Wash, dry and chop 10 g of purslane, add into 500 mL of water, add 0.15 g of enzyme and 0.3 g of cellulase, stir, heat to 40°C, enzymolyze for 1.5 h, inactivate the enzyme, filter, and retain the solid residue; freeze-dry the filtrate to obtain an extract.

[0074] Comparative Preparation Example 4

[0075] Compared with Preparation Example 2-3, the difference is that no alkaloid extract is added in step S5.

[0076] The details are as follows:

[0077] S5. Preparation of modified nanofiber membrane: 10 g of polyacrylonitrile was dissolved in 500 mL of dimethylformamide, 2.6 g of flavonoid extract was added, and the mixture was stirred for 30 min to obtain a spinning solution, which was spun using an electrospinning machine to obtain a nanofiber membrane, and then 0.6 g of probiotic microspheres was added to 50 mL of water and evenly sprayed on the surface of the nanofiber membrane to obtain a modified nanofiber membrane;

[0078] The spinning conditions are as follows: the syringe needle is 21G, the spinning solution pushing speed is 0.7mL / h, the spinning voltage is 17kV, the receiving distance is 15cm, and the receiving roller speed is 600r / min.

[0079] Comparative Preparation Example 5

[0080] Compared with Preparation Example 2-3, the difference is that no flavonoid extract is added in step S5.

[0081] The details are as follows:

[0082] S5. Preparation of modified nanofiber membrane: 10 g of polyacrylonitrile was dissolved in 500 mL of dimethylformamide, 2.6 g of alkaloid extract was added, and the mixture was stirred for 30 min to obtain a spinning solution, which was spun using an electrospinning machine to obtain a nanofiber membrane, and then 0.6 g of probiotic microspheres was added to 50 mL of water and evenly sprayed on the surface of the nanofiber membrane to obtain a modified nanofiber membrane;

[0083] The spinning conditions are as follows: the syringe needle is 21G, the spinning solution pushing speed is 0.7mL / h, the spinning voltage is 17kV, the receiving distance is 15cm, and the receiving roller speed is 600r / min.

[0084] Comparative Preparation Example 6

[0085] Compared with Preparation Example 2-3, the difference is that no probiotic microspheres are added in step S5.

[0086] The details are as follows:

[0087] S5. Preparation of nanofiber membrane: 10 g of polyacrylonitrile was dissolved in 500 mL of dimethylformamide, 1.4 g of alkaloid extract and 1.2 g of flavonoid extract were added, and the mixture was stirred for 30 min to obtain a spinning solution, which was then spun using an electrospinning machine to obtain a nanofiber membrane;

[0088] The spinning conditions are as follows: the syringe needle is 21G, the spinning solution pushing speed is 0.7mL / h, the spinning voltage is 17kV, the receiving distance is 15cm, and the receiving roller speed is 600r / min.

[0089] Comparative Preparation Example 7

[0090] Compared with Preparation Example 2-3, the difference is that the flavonoid extract is replaced by the extract.

[0091] The details are as follows:

[0092] S5. Preparation of modified nanofiber membrane: 10 g of polyacrylonitrile was dissolved in 500 mL of dimethylformamide, 1.4 g of alkaloid extract and 1.2 g of extract were added, and the mixture was stirred for 30 min to obtain a spinning solution, which was spun using an electrospinning machine to obtain a nanofiber membrane, and then 0.6 g of probiotic microspheres were added to 50 mL of water and evenly sprayed on the surface of the nanofiber membrane to obtain a modified nanofiber membrane;

[0093] The spinning conditions are as follows: the syringe needle is 21G, the spinning solution pushing speed is 0.7mL / h, the spinning voltage is 17kV, the receiving distance is 15cm, and the receiving roller speed is 600r / min.

[0094] Example 1

[0095] This embodiment provides a method for preparing a sanitary napkin composite chip, comprising the following steps: stacking a water-proof layer, a water-absorbing layer, and an anti-allergic soothing layer in order from bottom to top, and then compacting them for 5 minutes at a pressure of 10 MPa and a temperature of 80° C. to obtain a sanitary napkin composite chip;

[0096] The anti-allergic soothing layer is a nanofiber membrane loaded with probiotic microspheres and purslane extract prepared in Preparation Example 2-1, and has a thickness of 1 mm;

[0097] The waterproof layer is a polyethylene film or a polypropylene film with a thickness of 0.5 mm;

[0098] The water-absorbing layer is a composite layer of the silica aerogel and polyurethane sponge prepared in Preparation Example 1-1, and has a thickness of 2 mm.

[0099] Example 2

[0100] This embodiment provides a method for preparing a sanitary napkin composite chip, comprising the following steps: stacking a water-proof layer, a water-absorbing layer, and an anti-allergic soothing layer in order from bottom to top, and then compacting for 10 minutes at a pressure of 15 MPa and a temperature of 90° C. to obtain a sanitary napkin composite chip;

[0101] The anti-allergic soothing layer is a nanofiber membrane loaded with probiotic microspheres and purslane extract prepared in Preparation Example 2-2, and has a thickness of 1 mm;

[0102] The waterproof layer is a polyethylene film or a polypropylene film with a thickness of 0.5 mm;

[0103] The water-absorbing layer is a composite layer of the silica aerogel and polyurethane sponge prepared in Preparation Example 1-2, and has a thickness of 2 mm.

[0104] Example 3

[0105] The present embodiment provides a method for preparing a sanitary napkin composite chip, comprising the following steps: stacking a water-proof layer, a water-absorbing layer, and an anti-allergic soothing layer in order from bottom to top, and then compacting for 7 minutes at a pressure of 12 MPa and a temperature of 85° C. to prepare a sanitary napkin composite chip;

[0106] The anti-allergic soothing layer is a nanofiber membrane loaded with probiotic microspheres and purslane extract prepared in Preparation Example 2-3, and has a thickness of 1 mm;

[0107] The waterproof layer is a polyethylene film or a polypropylene film with a thickness of 0.5 mm;

[0108] The water-absorbing layer is a composite layer of the silica aerogel and polyurethane sponge prepared in Preparation Example 1-3, and has a thickness of 2 mm.

[0109] Comparative Example 1

[0110] Compared with Example 3, the difference is that the composite layer of silica aerogel and polyurethane sponge is replaced by the polyurethane sponge in Comparative Preparation Example 1.

[0111] Comparative Examples 2-7

[0112] Compared with Example 3, the difference is that the nanofiber membrane loaded with probiotic microspheres and purslane extract is replaced by the product prepared in Comparative Preparation Examples 2-7.

[0113] Test Example 1

[0114] The sanitary napkin composite chips prepared in Examples 1-3 of the present invention and Comparative Examples 1-7 were prepared into sanitary napkins, 100 mL of physiological saline containing 50 mg / mL hemoglobin was absorbed, and volunteers were asked to wear the sanitary napkins, which were removed after 2 hours. 20 professional personnel were asked to judge the odor of each group of sanitary napkins and collect whether the volunteers had allergic symptoms. The results are shown in Table 1.

[0115] Table 1

[0116]

[0117]

[0118] It can be seen from the above table that the sanitary napkin composite chips prepared in Examples 1-3 of the present invention have good deodorizing and odor-removing effects.

[0119] After the odor judgment is completed, the number of colonies on the sanitary napkin is measured. The surface layer of the sanitary napkin is cut into pieces, the chip is taken out, and then dispersed in 100 mL of deionized water, soaked for 3 hours, filtered, and 1 mL of the sample is cultured at 37°C for 48 hours to determine the total number of colonies. The results are shown in Table 2.

[0120] Table 2

[0121]

[0122] It can be seen from the above table that the sanitary napkin composite chips prepared in Examples 1-3 of the present invention have good antibacterial effects.

[0123] Test Example 2

[0124] The anti-inflammatory effect was evaluated by using lipopolysaccharide (LPS)-induced macrophage RAW264.7 to release inflammatory factor TNF-α cell model:

[0125] RAW264.7 cells were added to DMEM high-glucose culture medium containing 10% fetal bovine serum by volume and placed in an incubator (37°C, 5% CO2 by volume) for routine culture. When the cell confluence reached 80% to 90%, the cell suspension was taken and the cell density was adjusted to 7×10 4 / mL, added to a 24-well plate, 1000μL of cell suspension per well, placed in an incubator (37°C, 5% CO2) and cultured at a constant temperature for 24h. When the cell plating rate in the well plate meets the experimental requirements, the model group and the blank group are replaced with new complete culture medium, the positive control group is added with complete culture medium containing 100μg / mL dexamethasone, and the experimental group is added with culture medium containing sample solution (1cm 2 The sanitary napkin composite chip prepared in Example 1-3 or Comparative Example 1-7 was soaked in 50 mL of water for 2 hours, and the chip was removed to obtain a sample solution), and the cells were pretreated for 2 hours. Except for the blank group, the model group and the test group were added with a complete culture medium containing lipopolysaccharide solution (5 μg / mL) and continued to be incubated at 37°C for 16 hours. The supernatant was collected and centrifuged, and the supernatant was taken for TNF-α content detection according to the instructions of the ELISA kit. Each group of experiments was repeated 3 times and the average value was taken. The TNF-α inhibition rate (%) was calculated. The results are shown in Table 3.

[0126] TNF-α inhibition rate (%) = average TNF-α content in the experimental group / average TNF-α content in the model group × 100%

[0127] Table 3

[0128] Group TNF-α inhibition rate (%) Positive control group 45.6 Example 1 53.2 Example 2 55.7 Example 3 56.5 Comparative Example 1 52.7 Comparative Example 2 47.8 Comparative Example 3 42.1 Comparative Example 4 32.6 Comparative Example 5 37.9 Comparative Example 6 44.7 Comparative Example 7 39.6

[0129] It can be seen from the above table that the sanitary napkin composite chips prepared in Examples 1-3 of the present invention have good anti-inflammatory effects.

[0130] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An anti-allergic and soothing sanitary napkin composite chip containing purslane, characterized in that: The composite chip has a multi-layer structure, with a middle layer being a water-absorbing layer, an upper layer being an anti-allergic soothing layer, and a lower layer being a water-isolating layer; the anti-allergic soothing layer is a nanofiber membrane loaded with probiotic microspheres and purslane extract, and the water-isolating layer is a polyethylene film or a polypropylene film; the water-absorbing layer is a composite layer of silica aerogel and polyurethane sponge.

2. The sanitary napkin composite chip according to claim 1, characterized in that: The preparation method of the nanofiber membrane loaded with probiotic microspheres and purslane extract is as follows: S1. Enzyme-assisted fermentation of purslane: Wash, dry, and chop purslane, add to water, add sad enzyme and cellulase, stir for enzymolysis, add Lactobacillus plantarum and Bifidobacterium lactis seed solution, enzyme-assisted fermentation culture, filter, wash the solid, collect the bacterial solution, and retain the solid residue; freeze-dry the filtrate to obtain an extract; S2. Aqueous two-phase extraction: adding the solid residue to an aqueous solution of ethanol and ammonium sulfate, performing ultrasonic-assisted heating extraction, filtering, dialyzing the filtrate, and drying to obtain an alkaloid extract; S3. Macroporous resin purification: The extract was purified by macroporous resin to obtain a flavonoid extract; S4. Preparation of probiotic microspheres: adding sodium alginate and Tween to the bacterial solution, adding dropwise to the fish oil, emulsifying, adding dropwise calcium chloride solution, solidifying at room temperature, centrifuging, washing, and drying to obtain probiotic microspheres; S5. Preparation of modified nanofiber membrane: dissolve polyacrylonitrile in dimethylformamide, add alkaloid extract and flavonoid extract, stir and mix evenly to obtain spinning solution, use electrospinning machine to spin to obtain nanofiber membrane, then add probiotic microspheres into water, spray evenly on the surface of nanofiber membrane to obtain modified nanofiber membrane.

3. The sanitary napkin composite chip according to claim 2, characterized in that: In step S1, the mass ratio of purslane, enzyme and cellulase is 100:1-2:2-4, the temperature of the enzymolysis is 35-45°C, the time is 1-2h, and the bacterial content of the Lactobacillus plantarum and Bifidobacterium lactis seed solution is 10 8 -10 9 cfu / mL, the conditions of the enzyme-assisted fermentation culture are 36-38°C, 100-200r / min, and anaerobic conditions, and the enzyme-assisted fermentation culture is 24-36h, and the bacterial liquid has a bacterial content of 10 9 -10 10 cfu / mL.

4. The sanitary napkin composite chip according to claim 2, characterized in that: The solid-to-liquid ratio of the solid residue, ethanol and the aqueous solution of ammonium sulfate in step S2 is 1:5-10 g / mL, the ethanol content in the aqueous solution of ethanol and ammonium sulfate is 10-20wt%, the ammonium sulfate content is 12-15wt%, the ultrasonic power of the ultrasonic-assisted heating extraction is 1000-1500W, the heating temperature is 50-60°C, and the time is 2-4h.

5. The sanitary napkin composite chip according to claim 2, characterized in that: In step S3, the macroporous resin is selected from at least one of D-101, AB-8, and DA-201, and the eluent used for purification is a 45-65wt% ethanol solution.

6. The sanitary napkin composite chip according to claim 2, characterized in that: The mass ratio of the bacterial solution, sodium alginate and Tween in step S4 is 100:12-15:1-2, and the Tween is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80 and Tween-85.

7. The sanitary napkin composite chip according to claim 2, characterized in that: The mass ratio of polyacrylonitrile, alkaloid extract, flavonoid extract and probiotic microspheres in step S5 is 100:12-15:10-14:5-7, and the spinning conditions are: the syringe needle is 21G, the spinning solution push speed is 0.5-1mL / h, the spinning voltage is 15-20kV, the receiving distance is 10-20cm, and the receiving roller speed is 400-800r / min.

8. The sanitary napkin composite chip according to claim 1, characterized in that: The preparation method of the composite layer of silica aerogel and polyurethane sponge is as follows: adding silica aerogel to Tris-HCl solution, adding dopamine hydrochloride, heating and stirring to react, filtering, washing, drying to obtain modified silica aerogel, and uniformly spraying it on the surface of polyurethane sponge to obtain a composite layer.

9. The sanitary napkin composite chip according to claim 8, characterized in that: The pH value of the Tris-HCl solution is 8.5-9.5, the mass ratio of the silica aerogel to dopamine hydrochloride is 10:3-4, the temperature of the heating and stirring reaction is 40-50°C, the time is 2-3h, and the spraying amount of the modified silica aerogel is 0.5-1g / cm 2 .

10. A method for preparing the sanitary napkin composite chip according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: stacking a water-proof layer, a water-absorbing layer and an anti-allergic soothing layer in sequence from bottom to top, and then compacting them for 5-10 minutes at a pressure of 10-15Mpa and a temperature of 80-90°C to obtain a sanitary napkin composite chip.

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