51 results about "Vaginal flora" patented technology

The invention discloses lactobacillus crispatus for improving female genital tract microenvironment and immune aging and application of the lactobacillus crispatus, and belongs to the field of probiotics. The probiotic lactobacillus crispatus ProSci-38 disclosed by the invention can be used for effectively improving the symptoms such as pruritus vulvae, causalgia of vagina, abnormal vaginal secretion and the like of patients with vaginitis; mainly through the effect of intestinal tract-vaginal axis, the proportion of lactobacillus is increased, the proportion of harmful bacteria is reduced, the balance of vaginal flora is recovered, and the acidic environment of vagina is maintained; meanwhile, the inflammatory factor level can be reduced, the immune function of the vagina of a patient can be improved, so that the vagina environment is improved, the infection risk is reduced, and the preparation method has a great prospect in preparation of products for relieving and / or treating vaginitis.

Provided is a Lactobacillus crispatus VB250, which is deposited in the China General Microbiological Culture Collection Center (CGMCC) on 20 February 2023, with the deposit number of CGMCC No.26574. The Lactobacillus crispatus provided in the present invention has good growth activity and multiple pathogenic bacteria inhibition activity in a low pH environment, and can be used for preventing and / or relieving and / or treating related diseases caused by vaginal flora disorder, such as bacterial vaginitis, colpitis mycotica, trichomonas vaginitis, aerobic vaginitis, senile vaginitis, and vaginitis caused by virus infection. Moreover, the Lactobacillus crispatus has great application prospects in preparation of products such as food, health-care food, drugs, and hygienic products.

The invention belongs to the technical field of microorganisms, and particularly relates to combined lactobacillus salivarius and application thereof in female genital tract health. The preservation number of the strain is CCTCC NO: M20231719, and the strain has good bacteriostatic ability and dendritic cell (DC) maturation / activation stimulation ability, has no potential safety problem, can regulate vaginal micro-ecological balance, prevent infection caused by exogenous bacteria, effectively improve tumor immunosuppression microenvironment, and has good application prospects. And the method has very important significance on prevention / treatment of vaginal dysbacteriosis patients, gynecological tumors and precancerous lesions thereof.

The invention belongs to the technical field of preparation and application of probiotic postbiotics, and particularly relates to a composite probiotic postbiotic composition and application thereof. According to the compound probiotic metagen composition disclosed by the invention, the four probiotic metagens are compounded, so that vaginal harmful bacteria including bacteria and molds can be effectively inhibited, and the compound probiotic metagen composition has good in-vitro antibacterial activity; on the basis, the prebiotics are added into the composite probiotic postbiotic composition, so that the growth and stability of in-vivo lactobacillus inheritance can be promoted, the balance of vaginal flora can be recovered, and the diversity of species in microbial communities can be remarkably increased; meanwhile, symptoms such as vagina external redness and swelling, bleeding and pruritus caused by vaginitis can be effectively improved, no allergy phenomenon is generated, and therefore the compound can be applied to preparation of preparations for preventing and / or relieving vaginitis and can also be applied to production of antibacterial hygienic products, especially female hygienic products.

The present invention relates to an in vitro method for diagnosing Bacterial Vaginosis (BV) in a subject comprising: a) obtaining a biological sample from said subject, b) analysing the relative abundance of the host DNA in said sample and c) diagnosing that said subject suffers from bacterial vaginosis if said relative abundance is lower than a reference value. The present invention also relates to an in vitro method for diagnosing the severity of the vaginal dysbiosis in a subject, comprising the steps of: a) obtaining a biological sample from said subject, b) analysing the relative abundance of the host DNA in said sample and c) diagnosing that said subject has a severe vaginal dysbiosis if said relative abundance is lower than another reference value.

The application discloses an active oxygen response type bacteriostatic and repair promoting hydrogel and a preparation method and application thereof, the hydrogel is formed by disulfide bond cross-linked hydrogel in response to active oxygen free radicals in the vagina of vaginitis patients through thiol modified hyaluronic acid (HASH), and can enhance in-vivo retention by thiol and vaginal tissue interaction. Cationic surfactant such as didecyldimethylammonium chloride (DDAC) as the bacteriostatic component of the hydrogel, efficiently killing pathogenic bacteria while having good biocompatibility, and is not prone to cause bacterial drug resistance. As a natural immune regulator, beta-glucan regulates the vaginal microenvironment, promotes the polarization of macrophages into anti-inflammatory M2 type and promotes cell migration, etc., to promote the repair of the vaginal immune barrier, regulate the vaginal flora, and reduce the probability of vaginitis recurrence. The HASH hydrogel provides an efficient, convenient and highly transformative potential strategy to provide more choices for the treatment of vaginitis.

The embodiment of the invention provides a female vaginal flora structure typing method, device and equipment based on a consensus matrix and a storage medium, and relates to the technical field of biological detection.The method comprises the steps that based on extracted DNA of a plurality of female vaginal secretion swabs, PCR amplification is conducted through a 16s rRNA primer, sequencing and clustering are conducted on each amplified DNA, and the DNA of the female vaginal secretion swabs is obtained; the method comprises the following steps: generating ASV representative sequences, classifying and annotating the generated ASV representative sequences to obtain relative abundance matrix data of female vaginal flora, repeatedly sampling samples and strains of the relative abundance matrix data according to a set proportion, and clustering a plurality of generated subsets by adopting a plurality of clustering methods to obtain a plurality of clustering results; adopting a consensus matrix method to obtain a co-occurrence rate of any two samples in the relative abundance matrix data in a plurality of clustering results; all the obtained co-occurrence rates are clustered, and a flora CST typing result is obtained, so that the robustness and accuracy of female vagina flora structure typing are improved.

The invention discloses Lactobacillus crispatus and its use. The Lactobacillus crispatus is Lactobacillus crispatus chen-01 (Lactobacillus crispatus chen-01), and its deposit number is CGMCC NO.23396. Lactobacillus crispatus can be used to prepare medicines, and the medicines are used to treat and / or prevent HPV infection and vaginal infection. After the medicine prepared by the Lactobacillus crispatus is locally transplanted into the vagina of the present invention, the HPV viral load can be effectively reduced, the HPV clearance rate can be significantly improved, vaginitis can be effectively improved, and the diversity and abundance of vaginal flora can be significantly reduced; in addition, local vaginal transplantation of Lactobacillus crispatus can also significantly improve the vaginal microecological composition, which is close to that of normal healthy people.

The invention provides a vaginal flora repairing liquid and a preparation method thereof. Specifically, the repair liquid prepared from the lactobacillus paracasei CGMCC No. 1.12731, the lactobacillus rhamnosus CGMCC 24282 and the lactobacillus gasseri CGMCC No. 22909 in a specific mass ratio can be used for well killing pathogenic bacteria, has no obvious toxic or side effect and is low in cost. The broad-spectrum vaginitis bactericidal peptide YDG-C screened by the invention is further added into the repair liquid, so that the treatment effect on bacterial vaginitis can be further improved. The preparation method is simple, low in cost and suitable for large-scale popularization and application.

A hypotonic or iso-osmolal vaginal probiotic gel-forming formulation comprising: (a) vaginal microbiota, (b) a gel-forming polymer at a concentration at or below the critical gel concentration of the polymer under isotonic conditions and a temperature between room temperature and body temperature (25 to 37°C), and (c) a hypotonic carrier to form a hypotonic formulation of the polymer, wherein the vaginal microbiota is selected from the group consisting of Lactobacillus crispatus, Lactobacillus iners, Lactobacillus gasseri, or Lactobacillus jensenii. Said formulation for use in the treatment of bacterial vaginosis or a sexually transmitted disease.

In the treatment of VVC, Wan Dai Tang can eradicate fungi in the vagina, restore a vaginal microbial community structure, activate an STING signaling pathway, and protect mitochondria, thereby preventing the recurrence of vulvovaginal candidiasis. The Wan Dai Tang can significantly influence a CST V-type microbial population and a CST II-type microbial population in a vaginal microbial community, especially by improving the abundance of L. gasseri and L. jensenii in a vaginal flora. The Wan Dai Tang can also be applied to the maintenance of vaginal microecological balance.

The invention discloses skin mucosa maintenance liquid capable of killing fungi and HPV (human papillomavirus) as well as a preparation method and application of the skin mucosa maintenance liquid, and belongs to the technical field of sterilization and virus removal. The skin mucosa maintenance liquid is prepared from octanol ether, benzalkonium bromide, phenoxyethanol, yermate, parachlorometaxylenol, taetinic acid, chitosan, keratinase, fluconazole, glycerol, borneol, menthol, absolute ethyl alcohol and tween-40. Wherein the skin mucosa maintenance liquid has an effect of regulating apoptosis induction on abnormal cells and an effect on vaginal flora, and has an obvious inhibition effect on cervicitis and colpitis mycotica, namely an anti-inflammatory effect; meanwhile, apoptosis of cells containing HPV16 and HPV18 genes can be induced, shedding of abnormal cells is promoted, diseased cells are killed, and vaginal tissues with pathological changes are removed.

The invention discloses a mangosteen extract with a private care function and a preparation method and application thereof, and belongs to the technical field of cosmetics, the mangosteen extract can be widely applied to the preparation process of female private care products, has the effects of cleaning, inhibiting bacteria and adjusting vaginal flora imbalance, and can be used for treating private parts. Further, inflammatory reaction is reduced, and the self-cleaning capacity of the vagina is improved.

This invention provides a probiotic composition of Lab4 and Lactobacillus gasseri CUL09 and its application in reducing the risk of vaginal infections, belonging to the field of microbial application technology. Containing the Lab4 probiotic group and the novel strain Lactobacillus gasseri CUL09, this probiotic composition achieves vaginal flora reconstruction via oral administration, effectively improving vaginal pH, eliminating odor, and increasing lactobacillus colonization. This invention also relates to the combination of the probiotic composition with vitamin B6, cranberry extract, and conventional carriers, which can be used in the preparation of foods, dietary supplements, or pharmaceuticals for the prevention and / or treatment of vaginal and urethral microecological imbalances, vaginitis, vaginal candidiasis, and urethral infections. Furthermore, this invention, through the combination of Lab4 and Lactobacillus gasseri CUL09, achieves the inhibition of harmful vaginal bacteria, reconstruction of the vaginal flora to reduce the risk of vaginal infections, and can also prevent and treat urethral infections.

The invention discloses a compound preparation for regulating vaginal microecology as well as preparation and application thereof, and relates to the field of biological medicines. The composite preparation is composed of a fusion polypeptide and a monoclonal antibody according to the ratio of 1: 2 (w / w), the fusion polypeptide comprises a cell adhesion domain, an enzymolysis linking peptide and a quorum sensing inhibition domain, the amino acid sequence is shown as SEQ ID NO: 1, and the protease hydrolysis resistance is improved by more than 4 times after cyclization oxidation; the monoclonal antibody takes specific surface protein of lactobacillus inertus as a target spot, and can induce the strain to generate H2O2 phenotype conversion. An in-vitro experiment shows that the degradation rate of the composite preparation on a vaginal bacterial biofilm reaches 92.3 + / -2.4%, the ratio of lactobacillus acidophilus is remarkably increased to 63.8 + / -3.3%, and the pH is effectively adjusted to 4.3; animal experiments show that compared with commercially available metronidazole gel, the compound preparation can more remarkably promote proliferation of lactobacillus, inhibit escherichia coli and staphylococcus aureus and restore vaginal micro-ecological balance, and a new scheme is provided for treatment of vaginal micro-ecological imbalance related diseases.

The invention discloses a phototherapy nano system for removing candida and lactobacillus, a preparation method of the phototherapy nano system and application of the phototherapy nano system in preparation of drugs for treating vulvovaginal candidiasis. The phototherapy nano system comprises cell membranes with GPIb, GPIIb / IIIa and gC1q-R / P32 receptors; polylactic acid-glycolic acid copolymer (PLGA) nanoparticles wrapped by a cell membrane; a photosensitizer is embedded on the surface of the cell membrane; the preparation method comprises the following steps: extracting cell membrane PM of human platelets; the method comprises the following steps: premixing an IR780 photosensitizer and a cell membrane PM, and embedding IR780 into the cell membrane PM through ultrasonic under a water bath condition to obtain PM (IR); the preparation method comprises the following steps: carrying out water bath ultrasonic treatment on PM (IR) and polylactic acid-glycolic acid copolymer PLGA nanoparticles, and coating the PM (IR) on the polylactic acid-glycolic acid copolymer PLGA nanoparticles to obtain the PM (IR)-NP bionic nanoparticles. The problems of high recurrence rate, drug tolerance and vaginal flora ecological imbalance in the treatment of the recurrent vulvovaginal candidiasis can be relieved.

The invention provides a plant extraction gel for improving vaginal elasticity and a preparation method and application thereof, and belongs to the technical field of biological medicine. The plant extraction gel is prepared from fructus evodiae extract, Chinese herbaceous peony root extract, rhodomyrtus tomentosa fruit extract, dogwood extract, lime peel extract, cyrtomium rhizome extract, zedoary turmeric oil, melaleuca alternifolia oil, rosemary leaf oil, matrine and auxiliary materials. According to the invention, the fructus evodiae extract increases the thickness, the wettability and the elasticity of a vaginal wall by simulating an estrogen function; the Chinese herbaceous peony root extract has anti-inflammatory and anti-oxidation effects. The rhodomyrtus tomentosa fruit extract can tighten tissues in time, the dogwood extract can improve microcirculation and provide nutrition, and the lime peel extract can protect the elastic fibers from oxidative damage. All the components in the plant extraction gel supplement each other, and the comprehensive effect of recovering and maintaining vagina elasticity is achieved together. The plant extract gel disclosed by the invention has obvious effects in balancing vaginal flora and improving vaginal elasticity.

The invention discloses a lactic acid bacteria bacteriostatic composition, bacteriostatic gel and application. The lactic acid bacteria bacteriostatic composition is prepared from the following raw materials in parts by weight: 15 to 30 parts of lactic acid bacteria powder, 6 to 10 parts of rhizoma bletillae extract, 2 to 6 parts of herba centellae extract, 3 to 5 parts of fructus cnidii extract, 0.8 to 1 part of polyhexamethylene biguanide hydrochloride, 8 to 16 parts of stabilizer and 8 to 14 parts of additive. The lactic acid bacteria bacteriostatic composition has obvious anti-inflammatory and bacteriostatic effects, has a remarkable treatment effect on vaginitis and cervicitis, can effectively improve pathological injuries caused by vaginitis and cervicitis and promote repair of tissue mucosa, and can also promote expression of MKP-1 in cervical tissue, so that inflammatory response is improved, and the anti-inflammatory effect is achieved. Meanwhile, inflammation infiltration of the vagina of the rat is reduced to relieve the symptom of red and swollen vulva, the vaginal flora is protected, a favorable vaginal environment is created, the lactobacillus level is recovered to be normal, and harmful pathogenic bacteria can be inhibited in a targeted manner.

Described herein are methods and compositions for the use of treating and / or preventing vaginal bacterial infection and promoting healthy vaginal flora. Aspects of the invention relate to administering to a subject in need thereof a composition comprising a bacterial mixture of L. crispatus, L. gasseri, and L. jensenii.

The invention relates to a plant composition with a micro-ecological balance adjusting effect and application of the plant composition in female private products, and belongs to the technical field of biology. The preparation method of the plant polysaccharide fermentation product comprises the following steps: S1, extracting a plant composition with water to obtain a plant water extract; the plant composition is prepared from echinacea purpurea and circium japonicum; s2, filtering and concentrating the plant water extract in the step S1 to obtain a plant water extract concentrated solution; s3, carrying out alcohol precipitation on the plant water extract concentrated solution obtained in the step S2, and collecting the precipitate to obtain crude plant polysaccharide; and S4, fermenting the plant crude polysaccharide by using lactobacillus plantarum and lactobacillus delbrueckii to obtain a plant polysaccharide fermentation product. The echinacea and circium japonicum composition, the lactobacillus plantarum and the lactobacillus delbrueckii have a synergistic effect, polysaccharide substances are decomposed into oligosaccharide, disaccharide or monosaccharide, and by means of the prebiotics effect, female vaginal flora can be adjusted, and the micro-ecology of private parts can be balanced.

Utility model protection for an intimate protection system for the preventive shielding of the female urethra against ascending infectious agents, comprising a topically applicable protective agent in the form of a gel, a liquid, a paste or a sticker, characterized in that the composition • contains at least one active ingredient or combination of active ingredients that has antibacterial, antifungal, pH-stabilising or bioadhesive properties, • wherein the composition comprises in particular proanthocyanidins (PAC) in a proportion of 15 to 90%, preferably 30 to 50%, to inhibit the fimbrial binding of pathogenic microorganisms, • Contains D-Mannose in an optional amount to support the adhesion inhibition of Escherichia coli, • Lactic acid to stabilize the pH in the intimate area and to protect the vaginal flora, • Contains lactoferrin and enzymes in optional amounts for antibacterial and antifungal action, • Contains thickeners such as carbomer or xanthan to increase viscosity and adhesive strength, • Contains humectants, especially glycerin, to care for the mucous membrane, • Preservatives such as sodium benzoate, potassium sorbate or chelators (e.g. sodium EDTA) for preservation. • has a saliva-resistant formulation that remains stable on contact with saliva and thus forms a reliable barrier against oral germs, and the composition is designed to effectively shield the female urethra from ascending bacterial and fungal pathogens.

This application relates to the field of in vitro diagnostics and microbial detection technology, and in particular to a method and kit for optimizing reaction reagents for vaginal flora detection. The method includes serial dilution of 2×One-Step SYBR Green reagent and iScript reverse transcriptase in an RT-qPCR system, and supplementing the diluent with 0.2 mmol / L dNTPs and 1 mmol / L Mg. 2+ Achieving the reaction of 10 in an 8 μL reaction system 3 -10 8 Stable amplification of cellular equivalent RNA. This application reduces reagent usage to one-third of the original protocol while maintaining amplification efficiency, sensitivity, and reproducibility, significantly lowering detection costs and making it suitable for high-throughput screening and epidemiological studies.

The invention provides lactobacillus crispatus HGG35 for preventing and / or treating premature delivery and application of the lactobacillus crispatus HGG35, and belongs to the technical field of microorganisms. The lactobacillus crispatus HGG35 is preserved in the China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is November 30, 2023, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20232399. The lactobacillus crispatus HGG35 disclosed by the invention can produce hydrogen peroxide, L-lactic acid and D-lactic acid at high yield, has an obvious inhibition effect on common harmful strains in vagina, and can improve the balance of vaginal flora. The lactobacillus crispatus HGG35 disclosed by the invention has remarkable effects of preventing and treating premature delivery, and can be used for remarkably prolonging the pregnancy period, preventing premature delivery of fetuses and ensuring normal development of the fetuses.

The invention discloses a traditional Chinese medicine preparation for treating gynecological inflammation and a preparation method thereof.The traditional Chinese medicine preparation comprises a component A and a component B. The component A is prepared from cockscomb, motherwort, radix scrophulariae, radix rehmanniae recen, deer glue, turtle glue, processed ginger, bamboo charcoal powder, cortex phellodendri extract, lotus root extract, sodium alginate, honey and sesame oil; the component B comprises the following raw materials: fistular onion stalk, pepper, processed coptis chinensis, radix sophorae flavescentis and sargentgloryvine stem; the invention relates to the technical field of traditional Chinese medicine preparations. According to the traditional Chinese medicine preparation for treating gynecological inflammation and the preparation method thereof, through cooperation of the component A and the component B, the traditional Chinese medicine preparation not only has the advantages of sterilization and inflammation diminishing, but also is free of irritation through compatibility of pure traditional Chinese medicines, can nourish the vagina and provide effective support for recovery of vaginal flora, can effectively adsorb secretions in the vagina, and can effectively treat gynecological inflammation. Convenience is provided for vagina cleaning, and peculiar smell and pruritus at the vagina are effectively eliminated.

The utility model relates to a mesenchymal stem cell medical instrument technical field, especially through blood source mesenchymal stem cell collection device aspect. The device of collecting blood source mesenchymal stem cell has the function such as preventing vaginal flora pollution and is composed of the following structures: fixed ring (1), fixed ring attachment point (2), closed part (3), collection part (4), drainage part (6), the outer protective film (5) surrounding collection part and drainage part, the pulling handle (7) outside outer protective film and closed device (8) composition. In the process of collecting blood source mesenchymal stem cell, the pollution caused to the collection sample is avoided.

Provided is an agent for improving the balance of normal vaginal microbiota that is effective in keeping a favorable vaginal state. The present invention provides an agent for improving the balance of normal vaginal microbiota using sophorose and / or laminaribiose. Sophorose and / or laminaribiose have functionality for promoting the growth of beneficial bacteria that have a beneficial vaginal effect while suppressing the growth of bacteria that have the possibility of causing an adverse vaginal effect among normal vaginal microbiota. This agent for improving the balance of normal vaginal microbiota can be suitably used for the purpose of keeping a favorable vaginal state while maintaining the balance of normal vaginal microbiota.

The invention provides a novel application of an antibacterial peptide Ly (PEG)-1 reported at an earlier stage in vaginitis, belongs to the technical field of biomedical polypeptides, and particularly relates to preparation of the antibacterial peptide Ly (PEG)-1, an effect of the antibacterial peptide Ly (PEG)-1 on main vaginal pathogenic bacteria and symbiotic bacteria and an application of the antibacterial peptide Ly (PEG)-1 in prevention or treatment of bacterial vaginitis. The antibacterial peptide Ly (PEG)-1 provided by the invention is short in sequence, low in production cost, capable of strongly inhibiting main pathogenic bacteria of vagina, small in influence on main normal bacteria of the main pathogenic bacteria, capable of effectively treating vagina inflammation and maintaining the steady state of vaginal flora, high in stability and good in antibacterial effect. Vagina inflammatory diseases caused by infection of various pathogenic bacteria can be prevented and treated through various administration modes such as spraying, instillation, suppositories and effervescent tablets or systemic administration modes such as intraperitoneal injection and the like.

The invention relates to a genetic engineering technology, in particular to an engineering bacterium for expressing interferon alpha-2b and application of the engineering bacterium. According to the present invention, the Escherichia coli Nissle 1917 is subjected to gene engineering modification so as to obtain the engineering bacterium (Escherichia coli Nissle 1917 attB-INF alpha-2b) for expressing the interferon alpha-2b, such that the vagina inflammation of the HPV positive NSG mouse can be significantly reduced, and the vaginal flora of the HPV positive NSG mouse can be improved; the properties show that the engineering bacterium (Escherichia coli Nissle 1917 attB-INF alpha-2b) expressing the interferon alpha-2b can be used for treating human vagina inflammation caused by HPV (human papillomavirus) infection, and the steady state of vaginal flora is recovered.

The invention discloses a phycocyanin hydrogel capable of resisting endometritis and promoting vaginal flora regulation as well as a preparation method and application of the phycocyanin hydrogel. The preparation method comprises the following steps: a, adding a polylactic acid high-molecular polymer material into a sterile solvent, and performing low-temperature stirring reaction to obtain a hydrogel solution; and b, adding phycocyanin powder into the hydrogel solution prepared in the step a, and carrying out low-temperature stirring reaction to obtain the phycocyanin hydrogel. The hydrogel is phycocyanin hydrogel formed by wrapping natural phycocyanin with a polylactic acid derivative, accurate delivery in the genital tract can be achieved, and meanwhile, the anti-inflammatory and anti-apoptosis effects of phycocyanin can be fully exerted by slow release of the phycocyanin in the hydrogel; and the hydrogel can maintain an acidic environment in the vagina and regulate vaginal flora. The phycocyanin hydrogel disclosed by the invention is expected to bring a new treatment means for patients with endometritis and vaginal dysbacteriosis.

The invention relates to a cryopreservation protective solution suitable for vaginal flora of a human body, which mainly comprises the following components in percentage by weight: 10% of glycerol, 5% of D-trehalose, 0.2% of lactose, 1% of sodium lactate, 1% of hyaluronic acid, 1% of L-tartaric acid, 0.3% of sodium methyl p-hydroxybenzoate, and the balance of NaCl (sodium chloride), 10% of glycerol, 5% of D-trehalose, 0.2% of lactose, 1% of sodium lactate, 1% of hyaluronic acid, 1% of L-tartaric acid, 0.3% of sodium methyl p-hydroxybenzoate and the balance of water. The balance is NaCl with the concentration of 0.9%. The bacteria-preserving liquid is prepared according to the proportion and is uniformly mixed. The bacteria-preserving liquid can improve the preservation activity of vaginal probiotics.