25 results about "Vaginal flora" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to combined lactobacillus salivarius and application thereof in female genital tract health. The preservation number of the strain is CCTCC NO: M20231719, and the strain has good bacteriostatic ability and dendritic cell (DC) maturation / activation stimulation ability, has no potential safety problem, can regulate vaginal micro-ecological balance, prevent infection caused by exogenous bacteria, effectively improve tumor immunosuppression microenvironment, and has good application prospects. And the method has very important significance on prevention / treatment of vaginal dysbacteriosis patients, gynecological tumors and precancerous lesions thereof.

The application discloses an active oxygen response type bacteriostatic and repair promoting hydrogel and a preparation method and application thereof, the hydrogel is formed by disulfide bond cross-linked hydrogel in response to active oxygen free radicals in the vagina of vaginitis patients through thiol modified hyaluronic acid (HASH), and can enhance in-vivo retention by thiol and vaginal tissue interaction. Cationic surfactant such as didecyldimethylammonium chloride (DDAC) as the bacteriostatic component of the hydrogel, efficiently killing pathogenic bacteria while having good biocompatibility, and is not prone to cause bacterial drug resistance. As a natural immune regulator, beta-glucan regulates the vaginal microenvironment, promotes the polarization of macrophages into anti-inflammatory M2 type and promotes cell migration, etc., to promote the repair of the vaginal immune barrier, regulate the vaginal flora, and reduce the probability of vaginitis recurrence. The HASH hydrogel provides an efficient, convenient and highly transformative potential strategy to provide more choices for the treatment of vaginitis.

A hypotonic or iso-osmolal vaginal probiotic gel-forming formulation comprising: (a) vaginal microbiota, (b) a gel-forming polymer at a concentration at or below the critical gel concentration of the polymer under isotonic conditions and a temperature between room temperature and body temperature (25 to 37°C), and (c) a hypotonic carrier to form a hypotonic formulation of the polymer, wherein the vaginal microbiota is selected from the group consisting of Lactobacillus crispatus, Lactobacillus iners, Lactobacillus gasseri, or Lactobacillus jensenii. Said formulation for use in the treatment of bacterial vaginosis or a sexually transmitted disease.

The invention discloses skin mucosa maintenance liquid capable of killing fungi and HPV (human papillomavirus) as well as a preparation method and application of the skin mucosa maintenance liquid, and belongs to the technical field of sterilization and virus removal. The skin mucosa maintenance liquid is prepared from octanol ether, benzalkonium bromide, phenoxyethanol, yermate, parachlorometaxylenol, taetinic acid, chitosan, keratinase, fluconazole, glycerol, borneol, menthol, absolute ethyl alcohol and tween-40. Wherein the skin mucosa maintenance liquid has an effect of regulating apoptosis induction on abnormal cells and an effect on vaginal flora, and has an obvious inhibition effect on cervicitis and colpitis mycotica, namely an anti-inflammatory effect; meanwhile, apoptosis of cells containing HPV16 and HPV18 genes can be induced, shedding of abnormal cells is promoted, diseased cells are killed, and vaginal tissues with pathological changes are removed.

The invention discloses a mangosteen extract with a private care function and a preparation method and application thereof, and belongs to the technical field of cosmetics, the mangosteen extract can be widely applied to the preparation process of female private care products, has the effects of cleaning, inhibiting bacteria and adjusting vaginal flora imbalance, and can be used for treating private parts. Further, inflammatory reaction is reduced, and the self-cleaning capacity of the vagina is improved.

This invention provides a probiotic composition of Lab4 and Lactobacillus gasseri CUL09 and its application in reducing the risk of vaginal infections, belonging to the field of microbial application technology. Containing the Lab4 probiotic group and the novel strain Lactobacillus gasseri CUL09, this probiotic composition achieves vaginal flora reconstruction via oral administration, effectively improving vaginal pH, eliminating odor, and increasing lactobacillus colonization. This invention also relates to the combination of the probiotic composition with vitamin B6, cranberry extract, and conventional carriers, which can be used in the preparation of foods, dietary supplements, or pharmaceuticals for the prevention and / or treatment of vaginal and urethral microecological imbalances, vaginitis, vaginal candidiasis, and urethral infections. Furthermore, this invention, through the combination of Lab4 and Lactobacillus gasseri CUL09, achieves the inhibition of harmful vaginal bacteria, reconstruction of the vaginal flora to reduce the risk of vaginal infections, and can also prevent and treat urethral infections.

The invention discloses a compound preparation for regulating vaginal microecology as well as preparation and application thereof, and relates to the field of biological medicines. The composite preparation is composed of a fusion polypeptide and a monoclonal antibody according to the ratio of 1: 2 (w / w), the fusion polypeptide comprises a cell adhesion domain, an enzymolysis linking peptide and a quorum sensing inhibition domain, the amino acid sequence is shown as SEQ ID NO: 1, and the protease hydrolysis resistance is improved by more than 4 times after cyclization oxidation; the monoclonal antibody takes specific surface protein of lactobacillus inertus as a target spot, and can induce the strain to generate H2O2 phenotype conversion. An in-vitro experiment shows that the degradation rate of the composite preparation on a vaginal bacterial biofilm reaches 92.3 + / -2.4%, the ratio of lactobacillus acidophilus is remarkably increased to 63.8 + / -3.3%, and the pH is effectively adjusted to 4.3; animal experiments show that compared with commercially available metronidazole gel, the compound preparation can more remarkably promote proliferation of lactobacillus, inhibit escherichia coli and staphylococcus aureus and restore vaginal micro-ecological balance, and a new scheme is provided for treatment of vaginal micro-ecological imbalance related diseases.

The invention discloses a phototherapy nano system for removing candida and lactobacillus, a preparation method of the phototherapy nano system and application of the phototherapy nano system in preparation of drugs for treating vulvovaginal candidiasis. The phototherapy nano system comprises cell membranes with GPIb, GPIIb / IIIa and gC1q-R / P32 receptors; polylactic acid-glycolic acid copolymer (PLGA) nanoparticles wrapped by a cell membrane; a photosensitizer is embedded on the surface of the cell membrane; the preparation method comprises the following steps: extracting cell membrane PM of human platelets; the method comprises the following steps: premixing an IR780 photosensitizer and a cell membrane PM, and embedding IR780 into the cell membrane PM through ultrasonic under a water bath condition to obtain PM (IR); the preparation method comprises the following steps: carrying out water bath ultrasonic treatment on PM (IR) and polylactic acid-glycolic acid copolymer PLGA nanoparticles, and coating the PM (IR) on the polylactic acid-glycolic acid copolymer PLGA nanoparticles to obtain the PM (IR)-NP bionic nanoparticles. The problems of high recurrence rate, drug tolerance and vaginal flora ecological imbalance in the treatment of the recurrent vulvovaginal candidiasis can be relieved.

The invention provides a plant extraction gel for improving vaginal elasticity and a preparation method and application thereof, and belongs to the technical field of biological medicine. The plant extraction gel is prepared from fructus evodiae extract, Chinese herbaceous peony root extract, rhodomyrtus tomentosa fruit extract, dogwood extract, lime peel extract, cyrtomium rhizome extract, zedoary turmeric oil, melaleuca alternifolia oil, rosemary leaf oil, matrine and auxiliary materials. According to the invention, the fructus evodiae extract increases the thickness, the wettability and the elasticity of a vaginal wall by simulating an estrogen function; the Chinese herbaceous peony root extract has anti-inflammatory and anti-oxidation effects. The rhodomyrtus tomentosa fruit extract can tighten tissues in time, the dogwood extract can improve microcirculation and provide nutrition, and the lime peel extract can protect the elastic fibers from oxidative damage. All the components in the plant extraction gel supplement each other, and the comprehensive effect of recovering and maintaining vagina elasticity is achieved together. The plant extract gel disclosed by the invention has obvious effects in balancing vaginal flora and improving vaginal elasticity.

The invention discloses a lactic acid bacteria bacteriostatic composition, bacteriostatic gel and application. The lactic acid bacteria bacteriostatic composition is prepared from the following raw materials in parts by weight: 15 to 30 parts of lactic acid bacteria powder, 6 to 10 parts of rhizoma bletillae extract, 2 to 6 parts of herba centellae extract, 3 to 5 parts of fructus cnidii extract, 0.8 to 1 part of polyhexamethylene biguanide hydrochloride, 8 to 16 parts of stabilizer and 8 to 14 parts of additive. The lactic acid bacteria bacteriostatic composition has obvious anti-inflammatory and bacteriostatic effects, has a remarkable treatment effect on vaginitis and cervicitis, can effectively improve pathological injuries caused by vaginitis and cervicitis and promote repair of tissue mucosa, and can also promote expression of MKP-1 in cervical tissue, so that inflammatory response is improved, and the anti-inflammatory effect is achieved. Meanwhile, inflammation infiltration of the vagina of the rat is reduced to relieve the symptom of red and swollen vulva, the vaginal flora is protected, a favorable vaginal environment is created, the lactobacillus level is recovered to be normal, and harmful pathogenic bacteria can be inhibited in a targeted manner.

The invention relates to a plant composition with a micro-ecological balance adjusting effect and application of the plant composition in female private products, and belongs to the technical field of biology. The preparation method of the plant polysaccharide fermentation product comprises the following steps: S1, extracting a plant composition with water to obtain a plant water extract; the plant composition is prepared from echinacea purpurea and circium japonicum; s2, filtering and concentrating the plant water extract in the step S1 to obtain a plant water extract concentrated solution; s3, carrying out alcohol precipitation on the plant water extract concentrated solution obtained in the step S2, and collecting the precipitate to obtain crude plant polysaccharide; and S4, fermenting the plant crude polysaccharide by using lactobacillus plantarum and lactobacillus delbrueckii to obtain a plant polysaccharide fermentation product. The echinacea and circium japonicum composition, the lactobacillus plantarum and the lactobacillus delbrueckii have a synergistic effect, polysaccharide substances are decomposed into oligosaccharide, disaccharide or monosaccharide, and by means of the prebiotics effect, female vaginal flora can be adjusted, and the micro-ecology of private parts can be balanced.

Utility model protection for an intimate protection system for the preventive shielding of the female urethra against ascending infectious agents, comprising a topically applicable protective agent in the form of a gel, a liquid, a paste or a sticker, characterized in that the composition • contains at least one active ingredient or combination of active ingredients that has antibacterial, antifungal, pH-stabilising or bioadhesive properties, • wherein the composition comprises in particular proanthocyanidins (PAC) in a proportion of 15 to 90%, preferably 30 to 50%, to inhibit the fimbrial binding of pathogenic microorganisms, • Contains D-Mannose in an optional amount to support the adhesion inhibition of Escherichia coli, • Lactic acid to stabilize the pH in the intimate area and to protect the vaginal flora, • Contains lactoferrin and enzymes in optional amounts for antibacterial and antifungal action, • Contains thickeners such as carbomer or xanthan to increase viscosity and adhesive strength, • Contains humectants, especially glycerin, to care for the mucous membrane, • Preservatives such as sodium benzoate, potassium sorbate or chelators (e.g. sodium EDTA) for preservation. • has a saliva-resistant formulation that remains stable on contact with saliva and thus forms a reliable barrier against oral germs, and the composition is designed to effectively shield the female urethra from ascending bacterial and fungal pathogens.

This application relates to the field of in vitro diagnostics and microbial detection technology, and in particular to a method and kit for optimizing reaction reagents for vaginal flora detection. The method includes serial dilution of 2×One-Step SYBR Green reagent and iScript reverse transcriptase in an RT-qPCR system, and supplementing the diluent with 0.2 mmol / L dNTPs and 1 mmol / L Mg. 2+ Achieving the reaction of 10 in an 8 μL reaction system 3 -10 8 Stable amplification of cellular equivalent RNA. This application reduces reagent usage to one-third of the original protocol while maintaining amplification efficiency, sensitivity, and reproducibility, significantly lowering detection costs and making it suitable for high-throughput screening and epidemiological studies.

The utility model relates to a mesenchymal stem cell medical instrument technical field, especially through blood source mesenchymal stem cell collection device aspect. The device of collecting blood source mesenchymal stem cell has the function such as preventing vaginal flora pollution and is composed of the following structures: fixed ring (1), fixed ring attachment point (2), closed part (3), collection part (4), drainage part (6), the outer protective film (5) surrounding collection part and drainage part, the pulling handle (7) outside outer protective film and closed device (8) composition. In the process of collecting blood source mesenchymal stem cell, the pollution caused to the collection sample is avoided.

Provided is an agent for improving the balance of normal vaginal microbiota that is effective in keeping a favorable vaginal state. The present invention provides an agent for improving the balance of normal vaginal microbiota using sophorose and / or laminaribiose. Sophorose and / or laminaribiose have functionality for promoting the growth of beneficial bacteria that have a beneficial vaginal effect while suppressing the growth of bacteria that have the possibility of causing an adverse vaginal effect among normal vaginal microbiota. This agent for improving the balance of normal vaginal microbiota can be suitably used for the purpose of keeping a favorable vaginal state while maintaining the balance of normal vaginal microbiota.

The invention provides a novel application of an antibacterial peptide Ly (PEG)-1 reported at an earlier stage in vaginitis, belongs to the technical field of biomedical polypeptides, and particularly relates to preparation of the antibacterial peptide Ly (PEG)-1, an effect of the antibacterial peptide Ly (PEG)-1 on main vaginal pathogenic bacteria and symbiotic bacteria and an application of the antibacterial peptide Ly (PEG)-1 in prevention or treatment of bacterial vaginitis. The antibacterial peptide Ly (PEG)-1 provided by the invention is short in sequence, low in production cost, capable of strongly inhibiting main pathogenic bacteria of vagina, small in influence on main normal bacteria of the main pathogenic bacteria, capable of effectively treating vagina inflammation and maintaining the steady state of vaginal flora, high in stability and good in antibacterial effect. Vagina inflammatory diseases caused by infection of various pathogenic bacteria can be prevented and treated through various administration modes such as spraying, instillation, suppositories and effervescent tablets or systemic administration modes such as intraperitoneal injection and the like.

The invention discloses a phycocyanin hydrogel capable of resisting endometritis and promoting vaginal flora regulation as well as a preparation method and application of the phycocyanin hydrogel. The preparation method comprises the following steps: a, adding a polylactic acid high-molecular polymer material into a sterile solvent, and performing low-temperature stirring reaction to obtain a hydrogel solution; and b, adding phycocyanin powder into the hydrogel solution prepared in the step a, and carrying out low-temperature stirring reaction to obtain the phycocyanin hydrogel. The hydrogel is phycocyanin hydrogel formed by wrapping natural phycocyanin with a polylactic acid derivative, accurate delivery in the genital tract can be achieved, and meanwhile, the anti-inflammatory and anti-apoptosis effects of phycocyanin can be fully exerted by slow release of the phycocyanin in the hydrogel; and the hydrogel can maintain an acidic environment in the vagina and regulate vaginal flora. The phycocyanin hydrogel disclosed by the invention is expected to bring a new treatment means for patients with endometritis and vaginal dysbacteriosis.

The invention relates to a cryopreservation protective solution suitable for vaginal flora of a human body, which mainly comprises the following components in percentage by weight: 10% of glycerol, 5% of D-trehalose, 0.2% of lactose, 1% of sodium lactate, 1% of hyaluronic acid, 1% of L-tartaric acid, 0.3% of sodium methyl p-hydroxybenzoate, and the balance of NaCl (sodium chloride), 10% of glycerol, 5% of D-trehalose, 0.2% of lactose, 1% of sodium lactate, 1% of hyaluronic acid, 1% of L-tartaric acid, 0.3% of sodium methyl p-hydroxybenzoate and the balance of water. The balance is NaCl with the concentration of 0.9%. The bacteria-preserving liquid is prepared according to the proportion and is uniformly mixed. The bacteria-preserving liquid can improve the preservation activity of vaginal probiotics.

The invention provides a selective antibacterial composition as well as a preparation method and application thereof, and belongs to the technical field of sanitary or daily products. The invention provides a selective antibacterial composition which comprises a chlorhexidine antibacterial agent and a raspberry extract, and the mass ratio of the chlorhexidine antibacterial agent to the raspberry extract is (0.1-1): (1-10). The bacteriostatic composition with a certain ratio is innovatively provided, has a selective inhibition effect on vaginal flora, can regulate the vaginal microenvironment, has a good and rapid inhibition effect on common vaginal harmful bacteria such as escherichia coli, staphylococcus aureus and candida albicans, and has a good and rapid inhibition effect on common vaginal beneficial bacteria such as escherichia coli, staphylococcus aureus and candida albicans. For example, lactobacillus crispatus has a protective effect.

The invention relates to the technical field of female care products, and discloses a female care solution capable of balancing flora and a preparation method thereof.According to the care solution, water serves as a matrix and is compounded with a prebiotic component, a plant extract composite component, an antibacterial active component, a thickening stabilizer, a chelating agent, a preservative, a moisturizing solvent and a neutralizer; the synergistic effect of targeted regulation of vaginal flora, anti-inflammatory soothing and immunological enhancement is achieved through precise matching, the preparation method comprises the steps of multiphase step-by-step preparation, vacuum homogeneous mixing, precise neutralization, aging and the like, and activity retention of all the components and system stability are guaranteed. The technical problems that an existing care solution is poor in flora regulation pertinence, beneficial bacteria are damaged by a chemical antibacterial agent, and anti-inflammatory and flora balance synergy is insufficient are solved, and the care solution has the advantages of being accurate in flora regulation, mild, free of stimulation, stable in pH value, remarkable in anti-inflammatory relieving effect, good in immune enhancement and storage stability and the like and is suitable for daily care and auxiliary improvement of micro-ecological imbalance. Important clinical application and market values are realized.

The invention relates to the technical field of health-care food, in particular to a vagina probiotic composition which comprises the following raw materials: 10-20 parts of probiotic compound bacteria, 20-40 parts of prebiotics, 10-20 parts of traditional Chinese medicine powder and pharmaceutically acceptable auxiliary materials, wherein the probiotic compound bacteria are composed of lactobacillus reuteri strain GR-1, lactobacillus rhamnosus strain RC-14 and lactobacillus bulgaricus, and the traditional Chinese medicine powder is prepared from shikonin, hinokitiol and cicada slough extract. Three probiotics and traditional Chinese medicine powder are used in a combined mode, and the probiotics composite bacterial powder is used for balancing vaginal flora and preventing vaginitis from developing to urethritis; the shikonin, the hinokitiol and the cicada slough extract assist in improving urinary tract infection, inhibiting growth of pathogenic bacteria and avoiding further deterioration of vaginal flora; the prebiotics are used for proliferation of beneficial bacteria.

The application discloses application of a probiotic preparation in vaginal flora regulation and anti-HPV, and relates to the technical field of biological medicine; a pharmaceutical composition for assisting in treating high-risk human papilloma virus infection, the pharmaceutical composition comprising: interferon, which is recombinant human interferon alpha-2b; and a vaginal probiotic preparation, which comprises an effective dose of lactobacillus live bacteria; wherein the interferon and the probiotic preparation are formulated into a combined preparation or separate preparations for vaginal administration. The application discloses a new mechanism that the probiotic activates specific immune pathways to synergistically resist viruses, and proves excellent curative effects of the combined therapy; in traditional cognition, the probiotic mainly restores flora balance through occupying competition and acid production.

The invention provides a probiotic composition containing lactobacillus gasseri LN32 and lactobacillus crispatus LN70 and application of the probiotic composition to reduction of vaginal infection risk, and belongs to the technical field of microorganism application. The probiotic feed additive contains four strains of probiotics, including plant lactobacillus LN66, lactobacillus rhamnosus LN56, lactobacillus gasseri LN32 and lactobacillus crispatus LN70. The probiotic composition realizes vaginal flora reconstruction through oral administration, inhibits reproduction of Gardneria vaginalis and Candida albicans, increases lactobacillus colonization, and eliminates abnormal leucorrhea, vaginal scorching heat and itching, frequent urination, urgent urination, dysuria and the like. The probiotic composition is combined with a conventional carrier, and is applied to preparation of food, dietary supplement or medicine for preventing and / or treating microecological imbalance of vagina and urethra, bacterial vaginitis, colpitis mycotica and urethral infection. Therefore, vaginal harmful bacteria are inhibited, vaginal flora is reconstructed, and the vaginal infection risk and the urethral infection risk are reduced.

The present application provides a kind of lactobacillus gasseri LN32 and lactobacillus crispatus LN70 containing probiotic composition and application in reducing the risk of vaginal infection, belong to microbial application technical field.Containing 4 strains of probiotics, including lactobacillus plantarum LN66, lactobacillus rhamnosus LN56, lactobacillus gasseri LN32 and lactobacillus crispatus LN70.The probiotic composition is realized by oral route, vaginal flora reconstruction, inhibit the reproduction of gardnerella vaginalis and candida albicans, increase lactobacillus colonization, eliminate abnormal leucorrhea, vaginal burning and itching, frequent urination, urgency and pain.The probiotic composition is combined with conventional carrier, applied to prepare food, dietary supplement or drug for preventing and / or treating vaginal and urethral microecological disorder, bacterial vaginitis, mycotic vaginitis and urethral infection, and then the present application realizes inhibiting harmful bacteria in vagina, reconstructing vaginal flora to reduce the risk of vaginal infection and reduce the risk of urethral infection.

The invention discloses a vaginal flora protein marker group related to cervical high-level squamous intraepithelial lesions and application thereof, and adopts a metaproteomics method to construct vaginal flora protein markers for cervical HSIL diagnosis for the first time; the marker combination not only contributes to early recognition of cervical HSIL patients and optimization of clinical intervention strategies, but also provides an important basis for deep understanding of functional mechanisms of vaginal flora in cervical precancerous lesions and development of novel micro-ecological regulation therapy.