116 results about "Lactobacillus crispatus" patented technology

The invention provides lactobacillus crispatus, which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on February 7, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 26503. The bacillus subtilis can play a relatively strong role in inhibiting pathogenic bacteria such as gardnerella vaginalis, escherichia coli and staphylococcus aureus at the same time, and also has a certain role in inhibiting candida albicans, two-way proteurella and Atortoria vaginalis. Therefore, the lactobacillus crispatus has the potential of maintaining the environment in the vagina to be faintly acid and improving the mucosal immunity and infection resistance of a host, and can be used for preventing and treating vaginal infection and inflammation, and maintaining, improving or recovering the steady state of the environment in the vagina.

The invention relates to the technical field of microorganisms, and provides lactobacillus crispatus FMTA1145 C with a bacteriostatic effect and application of the lactobacillus crispatus FMTA1145 C. The lactobacillus crispatus FMTA1145 C is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 22, 2025, the address of the lactobacillus crispatus FMTA1145 C is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number of the lactobacillus crispatus FMTA1145 C is CGMCC NO.34293. The lactobacillus crispatus FMTA1145 C has the advantages that the lactobacillus crispatus FMTA1145 C has the antibacterial effect, and the lactobacillus crispatus FMTA1145 C has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 22, 2025; according to the technical scheme, the problem that the existing lactobacillus crispatus has a poor treatment effect on female vaginal diseases in the prior art is solved.

The invention relates to the field of biotechnology and female health care, in particular to a stachyose composition for promoting micro-ecological regulation of lactobacillus and application of the stachyose composition, and the composition comprises stachyose and pharmaceutically acceptable auxiliary materials. The stachyose is used as prebiotics to selectively promote proliferation and colonization of the lactobacillus crispatus, the combination of 1%-5% of the stachyose and 0.1%-0.5% of the lactic acid can promote proliferation of the lactobacillus crispatus, the lactobacillus crispatus is one or more of the lactobacillus crispatus, the lactobacillus plantarum and the lactobacillus acidophilus, and the combination of 1%-5% of the stachyose and 0.1%-0.5% of the lactic acid can inhibit gardnerella and candida albicans. The stachyose composition disclosed by the invention is in the form of slow-release gel and bath foam; the composition can be used for treating or preventing bacterial vaginitis, colpitis mycotica and related symptoms, has the advantages of no antibiotics, no drug resistance, high safety and the like, and is suitable for the fields of vagina local nursing and micro-ecological regulation.

The present disclosure provides isolated Lactobacillus crispatus, a composition comprising the same, and uses thereof, which can be used to treat, prevent, alleviate or alleviate liver and kidney diseases, metabolic diseases, and the like.

The invention discloses lactobacillus crispatus for relieving senescence by promoting collagen synthesis and a metagen of the lactobacillus crispatus, and belongs to the technical field of microorganisms and medicines. The lactobacillus crispatus CCFM1462 metagen provided by the invention has a good skin anti-aging effect, the content of collagen is increased, meanwhile, the skin moisture can be increased, and the skin oxidation resistance can be improved. Specifically, the enzyme activity of MMP-3 and MMP-1 of HSF cells can be inhibited in vitro by thallus lysate, and the content of COL I and COL III and the enzyme activity of LH1 can be improved in vitro from fermentation of supernate; the enzyme activity of MMP-1 and MMP-3 can be inhibited by orally taking inactivated thalli; the self-fermented supernate is orally taken, so that the enzyme activity of LH1 and LOX can be improved, the enzyme activity of MMP-3 can be reduced, and the anabolism of collagen can be regulated and controlled.

The invention provides a plurality of microbial markers for cervical cancer and an application of the microbial markers. The microbial markers comprise 11 bacteria, namely, Porphyromonas aeruginosa, Lactobacillus crispatus, Klebsiella pneumoniae, Gemerella haemolysms and the like, and the microbial markers comprise 11 bacteria, namely, the Porphyromonas aeruginosa, the Lactobacillus crispatus, the Klebsiella pneumoniae, the Gemerella haemolysms and the like. According to the method, the threshold value of the output risk value is obtained according to the trained random forest model, and then the probability value that the cervical lesion sample is the high-risk lesion can be obtained only by obtaining the relative abundance of the screened 11 genital tract microbial markers and inputting the relative abundance into the established random forest model. The machine learning model is constructed based on 16S sequencing, the high risk of cervical cancer can be recognized in time, the method is used for auxiliary diagnosis of cervical cancer patients, early discovery, early prevention and early treatment of cervical cancer are facilitated, and the accessibility of cervical cancer screening is improved.

The invention discloses a metagen which is prepared from zinc-rich lactobacillus crispatus CCFM1458 and has an effect of relieving acne vulgaris, and belongs to the technical field of microorganisms and medicines. The lactobacillus crispatus CCFM1458 provided by the invention has a zinc-rich function, can be used as an oral zinc protective agent, and has a huge application prospect in preparation of food, health care products or daily chemical products. The metagen prepared from the zinc-rich lactobacillus crispatus CCFM1458 has a good effect of relieving common acnes, can reduce the height and diameter of skin lesion cells of an individual acne, and can relieve inflammatory response and oxidative stress of skin. Therefore, the zinc-rich lactobacillus crispatus has a huge application prospect in preparation of external or oral medicines for relieving acne vulgaris.

The invention provides traditional Chinese medicine fermented disaccharide encapsulated nano anti-dandruff, hair-growing and oil-controlling shampoo and a preparation method, and relates to the field of daily chemical products, and the shampoo comprises the following components: a compound fermentation product, lactobacillus crispatus, lactobacillus plantarum, a disaccharide activity protection system, a basic component and a nano oil-controlling component; the compound leavening is prepared from cacumen biotae, radix polygoni multiflori, radix ginseng, fructus cnidii and orchid; the disaccharide activity protection system comprises maltotetraose and trehalose; the disaccharide activity protection system wraps a fermented material formed by fermenting the compound fermentation product, the lactobacillus crispatus and the lactobacillus plantarum to form a disaccharide glassy state layer; the nano oil control component is prepared from calamine powder and chitosan-sodium alginate; the basic component is prepared from tea bran, radix sophorae flavescentis, herba ecliptae, radix notoginseng, fructus ligustri lucidi, tea saponin microcapsules and tremella polysaccharide. According to the invention, through fermentation conversion of the orchid, disaccharide glassy state protection and slow release of the nano calamine, activity enhancement, stable retention and accurate release are realized, and the effects of removing dandruff, relieving itching, controlling oil and growing hair are achieved.

The invention provides a liver-protecting probiotic composition and application thereof in prevention or treatment of acute liver injury, and belongs to the technical field of microorganisms and medicines. The invention provides lactobacillus crispatus LJ003, and the preservation number of the lactobacillus crispatus LJ003 is CGMCC (China General Microbiological Culture Collection Center) No.30723. The invention further provides a method for preparing fermented globe artichoke leaf powder, polyphenol and chlorogenic acid by using the lactobacillus crispatus LJ003, and the fermented globe artichoke leaf powder prepared by using the lactobacillus crispatus LJ003. The prepared fermented globe artichoke leaf powder can be used for preparing the liver protection probiotic composition, and the obtained liver protection probiotic composition has a good protection effect on liver cells, can effectively improve liver cell lipid metabolism disorder caused by excessive ethanol intake, and has a remarkable effect on preventing or treating acute liver injury.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus crispatus M58D, and a composition, a pharmaceutical composition, a culture, a food product or a dietary supplement and a cleaning product containing the lactobacillus crispatus M58D. The invention also relates to application of the lactobacillus crispatus and the composition containing the lactobacillus crispatus in preparation of medicines.

The invention relates to the technical field of microorganisms, in particular to probiotics for improving female genital tract infection and application. The probiotics provided by the invention comprise at least one of plant lactobacillus plantarum VM-2, lactobacillus gasseri VL-3 and lactobacillus crispatus VX-4. The three strains have the effects of prevention and adjuvant therapy of female genital tract infectious diseases, and have very high probiotic potential. The invention also provides a combined product of the three strains, and growth inhibition experiments among the component strains ensure that the three strains can fully exert probiotic functions.

The present invention provides a probiotic active antibacterial tablet for preventing and treating female reproductive system infection and a preparation method thereof. The present invention is a probiotic active antibacterial tablet for preventing and treating female reproductive system infection, comprising the following raw materials in parts by weight: 1.8-2.5 parts of Lactobacillus reuteri, 1.0-1.2 parts of Lactobacillus rhamnosus, 1.2-1.7 parts of Lactobacillus crispatus, 0.5-0.8 parts of Lactobacillus gasseri, 1.0-1.5 parts of stachyose, 0.8-1.2 parts of inulin, and 2.5-3 parts of plant extract, wherein the plant extract is prepared from the following raw materials: Bletilla striata, Chinese tallow tree leaf, Gentiana scabra, and grapefruit seed. The probiotic active antibacterial tablet prepared by the present invention has a significant inhibitory effect on IGFBP7 protein, can effectively prevent and treat female reproductive system infection, and has good tolerance and safety.

The invention discloses lactobacillus crispatus CML521 and application thereof, and relates to the technical field of microorganisms, and the lactobacillus crispatus CML521 is technically characterized in that the lactobacillus crispatus CML521 is obtained through separation and culture from poultry craw contents, and the lactobacillus crispatus CML521 has remarkable biological characteristics and can be effectively used for preparing medicines for treating poultry candidiasis; the discovery of the lactobacillus crispatus CML521 provides a brand new treatment thought and method for poultry to treat candidiasis, and the newly discovered microbial strain not only can help poultry to resist the infringement of candidiasis, but also is expected to play an important role in the veterinary medicine field, and provides guarantee for poultry health.

The invention discloses a qi-tonifying and blood-nourishing composition and application thereof in formula milk powder, and belongs to the field of food. The composition provided by the invention comprises a plant fermentation product, heme iron, folic acid and ascorbic acid, and the plant fermentation product is prepared by taking angelica sinensis powder, ginseng powder, red date powder, red bean powder, red-skin peanut powder and medlar powder as raw materials, fermenting by aspergillus oryzae and lactobacillus crispatus, filtering, sterilizing and freeze-drying. A multi-strain mixed fermentation process is adopted, and the qi benefiting and blood nourishing effects of the composition can be effectively improved through the synergistic effect of different strains.

The application discloses a lactobacillus crispatus based on multiple targets to realize anti-aging effect and application of transformation of glycyrrhiza for efficiency enhancement, and belongs to the technical field of microorganisms and the technical field of medicine. The lactobacillus crispatus CCFM1362 provided in the application can ferment glycyrrhiza extract, can improve the content of glycyrrhizic acid, hemiglycyrrhizin isoflavone B, naringenin chalcone and other substances, can enhance the efficacy of glycyrrhiza extract, and can realize the effect of multiple targets for anti-aging, and the specific embodiments are as follows: (1) the content of beta-galactosidase in liver tissue is inhibited; (2) the level of aging-related secretory phenotype in liver tissue is reduced; (3) the Nrf2 related pathway is activated, and the level of oxidative stress is reduced; (4) the NF-kappa B related pathway is activated, and the level of chronic inflammation is reduced. Therefore, the lactobacillus crispatus CCFM1362 and the glycyrrhiza extract have great application prospect in the production of anti-aging products.

The invention relates to a preparation method, a product and application of a composite wormwood probiotic metagen, and the preparation method of the metagen comprises the following steps: inoculating lactobacillus gasseri TM13 and lactobacillus crispatus LG55 into a culture medium containing a wormwood extract, and fermenting to obtain fermentation liquor; and inactivating the fermentation liquor to obtain a finished product. The lactobacillus gasseri TM13 and the lactobacillus crispatus LG55 can mutually cooperate and cooperatively grow, and the wormwood extract is added in the fermentation process, so that the fermentation biomass can be remarkably increased, the pH value can be reduced, the bacteriostatic ability of a fermentation product on vaginal harmful bacteria can be improved, particularly, the candida albicans can be inhibited, and the synergistic interaction effect of the plant extract and the strain can be exerted. The form of a metagen product can break through the limitation of application of viable bacteria, external intervention can directly act on an affected part to regulate the micro-ecological environment of the vagina, loss caused by similar extreme environments of gastrointestinal tracts is avoided, and the effect is more prominent.

A method to reduce the likelihood of a dysbiosis of the vaginal microbiome in an individual employing a probiotic method and composition for maintaining a healthy vaginal microbiome, with particular embodiments including a bacterial formulation of three distinct species of Lactobacillus crispatus bacteria. In certain embodiments, the bacterial formulation is administered to an individual's vagina in an amount sufficient for the bacterial formulation to generate an amount of tryptophan metabolites sufficient to act as aryl hydrocarbon receptor (AHR) agonists, thereby reducing inflammation in the individual's vagina.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

Disclosed herein are methods and compositions related to probiotic formulas. These formulas comprise the bacterial strain Lactobacillus crispatus WZ-12 or a derivative thereof. Other strains which can be included in the composition include, but are not limited to, Lactobacillus salivarius 1-14, or Lactobacillus reuteri 2-2, and derivatives thereof. These compositions can be used in a variety of methods to treat and / or ameliorate diseases, such as colonization of antibiotic-resistant bacteria in the host organism. Also disclosed herein are methods of treating those subjects in need thereof by administering the compositions disclosed herein.

This application provides a *Lactobacillus curvatureii* bacteriocin, its preparation method, and its application, relating to the field of biotechnology. The *Lactobacillus curvatureii* bacteriocin provided in this application is obtained through gene recombination expression and is a natural antibacterial ingredient with a broader antibacterial spectrum, low irritation, and long-lasting effect. Applying this *Lactobacillus curvatureii* bacteriocin to feminine hygiene products not only enhances the product's antibacterial effect but also helps repair and maintain the balance of the microecology, preventing disruption of normal flora, effectively maintaining the acidic environment of the intimate area, inhibiting the growth of pathogenic bacteria, and reducing the risk of infection. The feminine hygiene product of this application combines gentleness, high efficacy, and multifunctionality, while also focusing on microecological balance and mucosal repair to meet the higher needs of women's intimate care.

A method of treating bacterial vaginosis in a subject is disclosed. The method comprises administering to the subject a therapeutically effective amount of a composition which comprises between three and twenty species of bacteria or a secretion thereof, wherein at least 70% of the bacteria of the composition are of the species Lactobacillus crispatus, thereby treating the bacterial vaginosis.

The present invention relates to the use of thiosulfate to potentiate the anti-pathogenic effect of lactobacilli bacteria, the thiosulfate being in a quantity of at least 100 mg for 107 to 1010 CFU of lactobacilli. The invention also relates to a pharmaceutical composition including at least 100 mg of thiosulfate per gram, in combination with a Lactobacillus crispatus strain, and to its use in the treatment of urogenital infections such as vaginosis, candidosis and urinary tract infections.

The compounding method comprises the following steps: A1, culturing lactobacillus and preparing a fermentation filtrate, namely inoculating the lactobacillus into a culture medium, culturing in an anaerobic environment with the temperature of 37 + / -1 DEG C and the pH value of 5.5-6.2, after culturing, carrying out centrifugal pretreatment and microfiltration membrane filtration, and collecting the fermentation product filtrate of which the main component is lactic acid; a2, preparing a compound solution, namely adding a pH buffer solution into a fermentation product filtrate while stirring, monitoring by using a pH meter with the precision of 0.01 until the pH is stabilized to 4-5, continuously stirring for 5-10 minutes to obtain the compound solution, and spraying or roll-coating the surface layer of a sanitary napkin base material with the compound solution to obtain the sanitary product. The growth of harmful bacteria such as antibacterial escherichia coli, staphylococcus aureus and candida albicans is inhibited, and the safety is high.

The invention relates to the technical field of probiotic application, in particular to application of lactobacillus crispatus HC1261 in preparation of a microbial agent for improving female reproductive health and a composition for improving vagina inflammation. The lactobacillus crispatus HC1261 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 16, 2023, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.27355; the microbial inoculum for improving female reproductive health can reduce the load of the ureaplasma urealyticum in the vagina. The lactobacillus crispatus HC1261 can be used for pertinently reducing the load of the ureaplasma urealyticum in the vagina, and can be used for reducing the related health risks of urethritis, cervicitis, pelvic inflammation, infertility and the like caused by the ureaplasma urealyticum.

This invention relates to the field of gene detection technology, specifically to a primer set and its application, and a training method for a bacterial vaginosis diagnostic model. The primer set, used for diagnosing bacterial vaginosis, includes specific primers for specifically amplifying target regions of the following pathogens: *Atopobacterium vaginalis*, *Gardnerella vaginalis*, *Cyclophorus 1*, *Cyclophorus 2*, *Bacterial Vaginosis-Associated Bacteria 2*, *Animal Curvularia clarithii*, *Animal Curvularia emblica*, *Prevotella 2*, *Lactobacillus curvularia*, *Lactobacillus indolentus*, *Lactobacillus gasseri*, and *Lactobacillus janniae*. This invention also provides the application of this primer set and a training method for a bacterial vaginosis diagnostic model. Using this primer set, high-throughput sequencing can be performed on test samples, simultaneously detecting 12 target pathogens, and further constructing a bacterial vaginosis diagnostic model, which can be used for the clinical diagnosis of bacterial vaginosis with good specificity and sensitivity.

The invention relates to the technical field of probiotics, in particular to lactobacillus crispatus with selenium enrichment capacity and an immunity improving function, application of the lactobacillus crispatus and a probiotic preparation. The lactobacillus crispatus HC1261 is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 16, 2023, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.27355, and the lactobacillus crispatus HC1261 is classified and named as lactobacillus crispatus. The lactobacillus crispatus HC1261 provided by the invention can enhance the immunity, the enhancement effect of the lactobacillus crispatus HC1261 on the immunity can be improved through selenium-enriched culture, and the lactobacillus crispatus HC1261 is good in safety and free of obvious toxic and side effects.

The present invention is related to at least one isolated bacterial strain selected from at least one of the species selected from the group comprising or, alternatively, consisting of: i) Lactobacillus plantarum, ii) Lactobacillus acidophilus, and iii) Lactobacillus crispatus, or mixtures of said at least one isolated bacterial strain thereof, for use in a method of metals gut microbial bioremediation. Preferably, said metals involved in the gut microbial bioremediation are heavy metals, which are selected from the group comprising or, alternatively, consisting of Ag, Be, Ba, Cd, Cr, Mn, Hg, Pb, Cu, V, and As preferably from Cd, Cr, Hg, and Pb; or Al, or mixtures thereof. In particular, said at least one isolated bacterial strain belonging to the specie i) Lactobacillus plantarum is selected from the group comprising or, alternatively, consisting of Lactiplantibacillus plantarum 476LL 20 bi LP01 (LMG P- 21021), Lactiplantibacillus plantarum 776 / 1 bi LP02 (LMG P-21020), and Lactiplantibacillus plantarum LP14 (DSM 33401) or mixtures thereof, said at least one isolated bacterial strain belonging to the specie ii) Lactobacillus acidophilus is selected from the group comprising or, alternatively, consisting of Lactobacillus acidophilus LA02 (DSM 21717), and Lactobacillus acidophilus LA06 (DSM 23033), and said at least one isolated bacterial strain belonging to the specie iii) Lactobacillus crispatus is identified as Lactobacillus crispatus LCR04 (DSM 33487). Furthermore, the present invention regards a mixture comprising at least one isolated bacterial strain as above indicated, as well as a composition comprising said mixture, for use in a method of metals gut microbial bioremediation, wherein said metals involved in the gut microbial bioremediation are heavy metals, which are selected from the group comprising or, alternatively, consisting of Ag, Be, Ba, Cd, Cr, Mn, Hg, Pb, Cu, V, and As preferably from Cd, Cr, Hg, and Pb; or Al, or mixtures thereof and in the prevention and or reduction of the harmful effects of heavy metals on gut health.

The present application relates to the technical field of microorganisms, in particular to a lactobacillus crispatus Lcr5-6 and application thereof, the lactobacillus crispatus Lcr5-6 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.38486;The adhesion rate of the lactobacillus crispatus Lcr5-6 to Hela cells is as high as 61.11%, which is significantly higher than that of the control strain lactobacillus delbrueckii DM8909 (12.13%), has excellent vaginal epithelial cell adhesion and colonization ability, and can be long-term colonization in the vagina to play a probiotic role.

The invention discloses a lactobacillus crispatus SX-E770 and application of the lactobacillus crispatus SX-E770 in preparation of products for reducing blood glucose and / or resisting oxidation, the preservation number of the lactobacillus crispatus SX-E770 is CCTCC NO: M 2025033, and the lactobacillus crispatus SX-E770 is preserved in China Center for Type Culture Collection on January 6, 2025. The novel lactobacillus crispatus strain SX-E770 provided by the invention simultaneously has excellent blood glucose reducing and oxidation resisting capabilities, can effectively reduce the content of glucose in a body, is good in safety, and has wide application prospects in the aspect of developing products such as probiotic preparations and medicines for regulating the blood glucose level, relieving oxidative damage and preventing and treating diabetes mellitus.

The present invention discloses an anti-aging composition for reducing structural dark circles and fine lines around the eyes and its application, and relates to the field of cosmetic technology. The present invention provides an anti-aging composition comprising the following components in parts by weight: 0.01-0.2 parts of Ruscus aconitifolia extract, 0.01-1 parts of Coleus forskohlii extract, 0.01-2 parts of μ-conopeptide, and 0.01-3 parts of a fermentation product of Lactobacillus crispatus; the Ruscus aconitifolia extract comprises ruscosapogenin C 27 H 42 O4 and neoruscosaponin C 27 H 40 The anti-aging composition provided by the present invention, when the four components of Ruscus aconitifolia extract, Coleus forskohlii extract, μ-conopeptide, and Lactobacillus crispatus fermentation product are used synergistically, can enhance skin firmness and elasticity, effectively reduce structural dark circles and smooth fine lines around the eyes, and the effect can be immediately and continuously achieved.