61 results about "Lactobacillus crispatus" patented technology

The invention relates to the field of biotechnology and female health care, in particular to a stachyose composition for promoting micro-ecological regulation of lactobacillus and application of the stachyose composition, and the composition comprises stachyose and pharmaceutically acceptable auxiliary materials. The stachyose is used as prebiotics to selectively promote proliferation and colonization of the lactobacillus crispatus, the combination of 1%-5% of the stachyose and 0.1%-0.5% of the lactic acid can promote proliferation of the lactobacillus crispatus, the lactobacillus crispatus is one or more of the lactobacillus crispatus, the lactobacillus plantarum and the lactobacillus acidophilus, and the combination of 1%-5% of the stachyose and 0.1%-0.5% of the lactic acid can inhibit gardnerella and candida albicans. The stachyose composition disclosed by the invention is in the form of slow-release gel and bath foam; the composition can be used for treating or preventing bacterial vaginitis, colpitis mycotica and related symptoms, has the advantages of no antibiotics, no drug resistance, high safety and the like, and is suitable for the fields of vagina local nursing and micro-ecological regulation.

The invention provides traditional Chinese medicine fermented disaccharide encapsulated nano anti-dandruff, hair-growing and oil-controlling shampoo and a preparation method, and relates to the field of daily chemical products, and the shampoo comprises the following components: a compound fermentation product, lactobacillus crispatus, lactobacillus plantarum, a disaccharide activity protection system, a basic component and a nano oil-controlling component; the compound leavening is prepared from cacumen biotae, radix polygoni multiflori, radix ginseng, fructus cnidii and orchid; the disaccharide activity protection system comprises maltotetraose and trehalose; the disaccharide activity protection system wraps a fermented material formed by fermenting the compound fermentation product, the lactobacillus crispatus and the lactobacillus plantarum to form a disaccharide glassy state layer; the nano oil control component is prepared from calamine powder and chitosan-sodium alginate; the basic component is prepared from tea bran, radix sophorae flavescentis, herba ecliptae, radix notoginseng, fructus ligustri lucidi, tea saponin microcapsules and tremella polysaccharide. According to the invention, through fermentation conversion of the orchid, disaccharide glassy state protection and slow release of the nano calamine, activity enhancement, stable retention and accurate release are realized, and the effects of removing dandruff, relieving itching, controlling oil and growing hair are achieved.

The application discloses a lactobacillus crispatus based on multiple targets to realize anti-aging effect and application of transformation of glycyrrhiza for efficiency enhancement, and belongs to the technical field of microorganisms and the technical field of medicine. The lactobacillus crispatus CCFM1362 provided in the application can ferment glycyrrhiza extract, can improve the content of glycyrrhizic acid, hemiglycyrrhizin isoflavone B, naringenin chalcone and other substances, can enhance the efficacy of glycyrrhiza extract, and can realize the effect of multiple targets for anti-aging, and the specific embodiments are as follows: (1) the content of beta-galactosidase in liver tissue is inhibited; (2) the level of aging-related secretory phenotype in liver tissue is reduced; (3) the Nrf2 related pathway is activated, and the level of oxidative stress is reduced; (4) the NF-kappa B related pathway is activated, and the level of chronic inflammation is reduced. Therefore, the lactobacillus crispatus CCFM1362 and the glycyrrhiza extract have great application prospect in the production of anti-aging products.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

Disclosed herein are methods and compositions related to probiotic formulas. These formulas comprise the bacterial strain Lactobacillus crispatus WZ-12 or a derivative thereof. Other strains which can be included in the composition include, but are not limited to, Lactobacillus salivarius 1-14, or Lactobacillus reuteri 2-2, and derivatives thereof. These compositions can be used in a variety of methods to treat and / or ameliorate diseases, such as colonization of antibiotic-resistant bacteria in the host organism. Also disclosed herein are methods of treating those subjects in need thereof by administering the compositions disclosed herein.

This application provides a *Lactobacillus curvatureii* bacteriocin, its preparation method, and its application, relating to the field of biotechnology. The *Lactobacillus curvatureii* bacteriocin provided in this application is obtained through gene recombination expression and is a natural antibacterial ingredient with a broader antibacterial spectrum, low irritation, and long-lasting effect. Applying this *Lactobacillus curvatureii* bacteriocin to feminine hygiene products not only enhances the product's antibacterial effect but also helps repair and maintain the balance of the microecology, preventing disruption of normal flora, effectively maintaining the acidic environment of the intimate area, inhibiting the growth of pathogenic bacteria, and reducing the risk of infection. The feminine hygiene product of this application combines gentleness, high efficacy, and multifunctionality, while also focusing on microecological balance and mucosal repair to meet the higher needs of women's intimate care.

The compounding method comprises the following steps: A1, culturing lactobacillus and preparing a fermentation filtrate, namely inoculating the lactobacillus into a culture medium, culturing in an anaerobic environment with the temperature of 37 + / -1 DEG C and the pH value of 5.5-6.2, after culturing, carrying out centrifugal pretreatment and microfiltration membrane filtration, and collecting the fermentation product filtrate of which the main component is lactic acid; a2, preparing a compound solution, namely adding a pH buffer solution into a fermentation product filtrate while stirring, monitoring by using a pH meter with the precision of 0.01 until the pH is stabilized to 4-5, continuously stirring for 5-10 minutes to obtain the compound solution, and spraying or roll-coating the surface layer of a sanitary napkin base material with the compound solution to obtain the sanitary product. The growth of harmful bacteria such as antibacterial escherichia coli, staphylococcus aureus and candida albicans is inhibited, and the safety is high.

The invention relates to the technical field of probiotic application, in particular to application of lactobacillus crispatus HC1261 in preparation of a microbial agent for improving female reproductive health and a composition for improving vagina inflammation. The lactobacillus crispatus HC1261 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 16, 2023, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.27355; the microbial inoculum for improving female reproductive health can reduce the load of the ureaplasma urealyticum in the vagina. The lactobacillus crispatus HC1261 can be used for pertinently reducing the load of the ureaplasma urealyticum in the vagina, and can be used for reducing the related health risks of urethritis, cervicitis, pelvic inflammation, infertility and the like caused by the ureaplasma urealyticum.

This invention relates to the field of gene detection technology, specifically to a primer set and its application, and a training method for a bacterial vaginosis diagnostic model. The primer set, used for diagnosing bacterial vaginosis, includes specific primers for specifically amplifying target regions of the following pathogens: *Atopobacterium vaginalis*, *Gardnerella vaginalis*, *Cyclophorus 1*, *Cyclophorus 2*, *Bacterial Vaginosis-Associated Bacteria 2*, *Animal Curvularia clarithii*, *Animal Curvularia emblica*, *Prevotella 2*, *Lactobacillus curvularia*, *Lactobacillus indolentus*, *Lactobacillus gasseri*, and *Lactobacillus janniae*. This invention also provides the application of this primer set and a training method for a bacterial vaginosis diagnostic model. Using this primer set, high-throughput sequencing can be performed on test samples, simultaneously detecting 12 target pathogens, and further constructing a bacterial vaginosis diagnostic model, which can be used for the clinical diagnosis of bacterial vaginosis with good specificity and sensitivity.

The invention relates to the technical field of probiotics, in particular to lactobacillus crispatus with selenium enrichment capacity and an immunity improving function, application of the lactobacillus crispatus and a probiotic preparation. The lactobacillus crispatus HC1261 is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 16, 2023, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.27355, and the lactobacillus crispatus HC1261 is classified and named as lactobacillus crispatus. The lactobacillus crispatus HC1261 provided by the invention can enhance the immunity, the enhancement effect of the lactobacillus crispatus HC1261 on the immunity can be improved through selenium-enriched culture, and the lactobacillus crispatus HC1261 is good in safety and free of obvious toxic and side effects.

The present invention is related to at least one isolated bacterial strain selected from at least one of the species selected from the group comprising or, alternatively, consisting of: i) Lactobacillus plantarum, ii) Lactobacillus acidophilus, and iii) Lactobacillus crispatus, or mixtures of said at least one isolated bacterial strain thereof, for use in a method of metals gut microbial bioremediation. Preferably, said metals involved in the gut microbial bioremediation are heavy metals, which are selected from the group comprising or, alternatively, consisting of Ag, Be, Ba, Cd, Cr, Mn, Hg, Pb, Cu, V, and As preferably from Cd, Cr, Hg, and Pb; or Al, or mixtures thereof. In particular, said at least one isolated bacterial strain belonging to the specie i) Lactobacillus plantarum is selected from the group comprising or, alternatively, consisting of Lactiplantibacillus plantarum 476LL 20 bi LP01 (LMG P- 21021), Lactiplantibacillus plantarum 776 / 1 bi LP02 (LMG P-21020), and Lactiplantibacillus plantarum LP14 (DSM 33401) or mixtures thereof, said at least one isolated bacterial strain belonging to the specie ii) Lactobacillus acidophilus is selected from the group comprising or, alternatively, consisting of Lactobacillus acidophilus LA02 (DSM 21717), and Lactobacillus acidophilus LA06 (DSM 23033), and said at least one isolated bacterial strain belonging to the specie iii) Lactobacillus crispatus is identified as Lactobacillus crispatus LCR04 (DSM 33487). Furthermore, the present invention regards a mixture comprising at least one isolated bacterial strain as above indicated, as well as a composition comprising said mixture, for use in a method of metals gut microbial bioremediation, wherein said metals involved in the gut microbial bioremediation are heavy metals, which are selected from the group comprising or, alternatively, consisting of Ag, Be, Ba, Cd, Cr, Mn, Hg, Pb, Cu, V, and As preferably from Cd, Cr, Hg, and Pb; or Al, or mixtures thereof and in the prevention and or reduction of the harmful effects of heavy metals on gut health.

The present application relates to the technical field of microorganisms, in particular to a lactobacillus crispatus Lcr5-6 and application thereof, the lactobacillus crispatus Lcr5-6 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.38486;The adhesion rate of the lactobacillus crispatus Lcr5-6 to Hela cells is as high as 61.11%, which is significantly higher than that of the control strain lactobacillus delbrueckii DM8909 (12.13%), has excellent vaginal epithelial cell adhesion and colonization ability, and can be long-term colonization in the vagina to play a probiotic role.

The invention discloses lactobacillus crispatus CZ02 resistant to gastrointestinal digestion, a high-density fermentation method thereof and application of the lactobacillus crispatus CZ02 in female reproductive health, and belongs to the technical field of microorganisms. On one hand, the invention provides the lactobacillus crispatus CZ02 resistant to gastrointestinal digestion, and on the other hand, the invention provides application and a high-density fermentation method of the lactobacillus crispatus CZ02. The lactobacillus crispatus CZ02 provided by the invention has a relatively high survival rate in a low pH value, and can resist a strong acid environment. The lactobacillus crispatus disclosed by the invention has good acid resistance, artificial gastrointestinal fluid tolerance and cholate tolerance, and the lactobacillus crispatus disclosed by the invention has relatively strong tolerance in different polar environments, has better stability in a hydrophobic or high polar environment, and is suitable for being planted in intestinal tracts. The lactobacillus crispatus CZ02 disclosed by the invention can migrate from intestinal tracts to vaginas for colonization, can inhibit growth of pathogenic microorganisms and regulate flora balance in the vaginas, so that related diseases caused by vaginal flora disorder can be prevented and / or treated.

The invention discloses lactobacillus crispatus CCFM1462 and application of a metagen of the lactobacillus crispatus CCFM1462 in promoting skin ceramide synthesis by regulating a sphingomyelinase pathway, and belongs to the technical field of microorganisms and medicines. The lactobacillus crispatus CCFM1462 provided by the invention can effectively improve the content of ceramide in the aspects of external use and oral administration. According to the application disclosed by the invention, it is found that a metagen (fermented supernatant CCFM1462-S) prepared by oral administration of lactobacillus crispatus CCFM1462 can promote expression of sphingomyelinase, so that a sphingomyelinase pathway in a ceramide synthesis process is regulated and controlled, and rapid supplement of ceramide is realized. Therefore, the lactobacillus crispatus plays a crucial role in solving the problem that ceramide needs to be supplemented emergently after skin irritation, has a huge application prospect when being applied to oral or external medicines, and is used for transient stress repair of skin.

The invention provides an anxiety relieving and sleep assisting strain of Weizia coagulans and application of the anxiety relieving and sleep assisting strain of Weizia coagulans, the Weizia coagulans is Weizia coagulans CN365, the Weizia coagulans is preserved in China Center for Type Culture Collection, the preservation date is October 28, 2025, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20252374. The invention provides a brand-new strain of Witzia ciliaris CN365, which has the effects of relieving anxiety and assisting sleep. Experimental results show that the bacillus coagulans CN365 is resistant to high temperature and high in yield of indole lactic acid, and particularly suitable prebiotics are added, so that high yield of indole lactic acid can be remarkably promoted. Freeze-dried powder is prepared, and the activity of the bacterial powder is high; besides, the Witzia ciliaris CN365 can improve anxiety, improve sleep disorders and promote calcium transport and absorption, and especially when the Witzia ciliaris CN365 and the Lactobacillus crispatus GG77 are combined for use, the Witzia ciliaris CN365 and the Lactobacillus crispatus GG77 have a synergistic interaction effect, and the transport and absorption effect on calcium ions can be significantly improved.

The invention discloses an application of a bacterial strain in production of an external product for improving the micro-ecology of the vagina of a human body, and the bacterial strain is Lactobacillus crispatus SPR005 and has 16S rDNA (ribosomal deoxyribonucleic acid) as shown in SEQ ID NO.1. The invention also discloses an application of the bacterial strain in production of the external product for improving the micro-ecology of the vagina of the human body. The preservation number is GDMCC NO: 66665; the strain produces H2O2 and only produces D-lactic acid, and no drug-resistant gene is found; the external product is suitable for improving changes, caused by vaginal pathogenic bacteria, of micro-ecology of the vagina of the human body. Compared with other lactobacillus crispatus of the same type, the strain disclosed by the invention can generate a strong bacteriostatic effect on staphylococcus aureus when the concentration of the strain is about 1 * 10 < 7 > CFU / ml, and the bacteriostatic rate reaches 99.78 or above. In addition, the strain disclosed by the invention is relatively high in acid production capacity, and the total amount of D-lactic acid produced in 48 hours is 16.28 mM; the D-lactic acid not only can effectively reduce the pH value of the vagina and maintain the pH value of the vagina between 3.8 and 4.0, but also can enhance the recognition and removal capability of pathogens by adjusting the immune response of the host. D-lactic acid can inhibit the growth of fungi, such as candida albicans; the D-lactic acid level is also positively correlated with the vaginal homeostasis, so that the treatment of BV patients is facilitated.

The present disclosure provides isolated Lactobacillus strains, compositions comprising the same, and uses thereof, which can be used for promoting growth and development, enhancing immunity, or promoting intestinal health, etc.

The invention discloses application of 2-hydroxycinnamic acid in treatment of gastric cancer, according to the application, the influence of supernate of lactobacillus salivarius or lactobacillus crispatus on gastric cancer cell apoptosis is detected through a flow technology, and the 2-hydroxycinnamic acid is found to be capable of promoting the gastric cancer cell apoptosis and inhibiting the activity of gastric cancer organs. A metabolite in a supernatant of lactobacillus salivarius CICC23175 or lactobacillus crispatus CGMCC1.2743 screened by non-target metabolite omics contains 2-hydroxycinnamic acid, and the gastric cancer cytotoxic resistance of 2-hydroxycinnamic acid is deeply studied to find that the 2-hydroxycinnamic acid not only has the effects of inhibiting gastric cancer cell proliferation, colony formation and cell migration, but also has the effects of inhibiting gastric cancer cell proliferation, colony formation and cell migration. In addition, the 2-hydroxycinnamic acid can also increase the proportion of cells in a G1 / G0 period of a cell cycle, promote apoptosis of gastric cancer cells, and find that the inhibition effect of the 2-hydroxycinnamic acid on proliferation of gastric cancer organs is integrally higher than that of normal gastric epithelium organs.

The invention relates to the technical field of microorganisms, and particularly discloses lactobacillus crispatus and application thereof in regulating blood sugar, improving cognitive impairment of diabetes and treating vaginitis. In the invention, the lactobacillus crispatus A21013 and the lactobacillus crispatus A21033 are found to have multiple effects for the first time, can reduce blood sugar, maintain forms and functions of pancreatic beta cells, improve cognitive impairment of diabetes, and inhibit growth and reproduction of vaginal harmful pathogens and oral pathogenic bacteria, so that the lactobacillus crispatus A21013 and the lactobacillus crispatus A21033 can be used for treating diabetes mellitus. A dual prevention and treatment means is provided for patients with diabetes, cognitive impairment and vaginitis.

Postbiotic compositions for maintenance and / or restoration of a healthy vaginal environment and / or microbiome are disclosed. The postbiotic compositions include a paraprobiotic component, comprising inactivated cells of Lactobacillus crispatus, and a metabolite component, comprising D-lactate.

The present application relates to a Lactobacillus crispatus X25B and uses thereof. In particular, the present application relates to a Lactobacillus crispatus, as well as compositions, pharmaceutical compositions, cultures, food products or dietary supplements and cleaning products comprising the same. The present application also relates to the use of said Lactobacillus crispatus and compositions comprising the same for the manufacture of a medicament.

This invention belongs to the field of microbial technology, specifically relating to the multifunctional Lactobacillus curvatureii and its application in the preparation of health products. The Lactobacillus curvatureii (… Lactobacillus crispatus Lactobacillus crispatus The accession number for MCOlact00102 is CGMCC No. 34381. The described *Lactobacillus curvatureensis* (MCOlact00102) is... Lactobacillus crispatus ​ The two strains, MCOlact00126 (CGMCC No. 34382), exhibit significant inhibitory effects against bacterial vaginosis (BV) and infections caused by Gardnerella vaginalis, Group B Streptococcus (GBS), Escherichia coli, or Candida albicans. The safety of these strains was evaluated using a 3D vaginal epithelial organoid model, showing no irritant effect on cells. Furthermore, hemolytic activity tests, antibiotic resistance gene detection, and functional validation all demonstrated their good safety profile. In addition, the aerobic acclimation process significantly improved the stability and bioactivity of these two strains in actual production and clinical applications, laying a solid foundation for their industrialization and application.

The present disclosure provides isolated lactobacillus crispatus, compositions comprising the same, and uses thereof, which can be used to treat, prevent, ameliorate, or alleviate hepatorenal disease, metabolic disease, etc.

This invention discloses a strain of *Lactobacillus crispatus* push, classified as *Lactobacillus crispatus* push, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 35610, on August 13, 2025. The culture conditions were: 37°C, anaerobic, and 48 hours. This *Lactobacillus crispatus* strain exhibits significant advantages in inhibiting the growth of HPV-infected cervical epithelial cells and shows great potential in the treatment of HPV infection in the female lower genital tract.

The invention discloses an application of lactobacillus in treatment of gastric cancer. The lactobacillus is at least one of lactobacillus salivarius with the preservation number of CICC23175 and lactobacillus crispatus with the preservation number of CGMCC1.2743. The lactobacillus is at least one of lactobacillus salivarius with the preservation number of CICC23175 and lactobacillus crispatus with the preservation number of CGMCC1.2743. According to the present invention, the lactobacillus salivarius CICC23175 or the lactobacillus crispatus CGMCC1.2743, which has the excellent inhibition effect on the proliferation of SGC7901, AGS and MKN45 gastric cancer cells, is found; a mouse solid tumor model experiment shows that the lactobacillus salivarius CICC23175 or the lactobacillus crispatus CGMCC1.2743 can be used for inhibiting the growth of gastric cancer transplanted tumors. According to the invention, viable bacteria of lactobacillus salivarius CICC23175 or lactobacillus crispatus CGMCC1.2743 in lactobacillus are directly applied to treatment of gastric cancer for the first time, and a new direction and basis can be provided for treatment of gastric cancer.

The present invention relates to the use of selected UV filters selected from the group consisting of octocrylene, ethyl hexyltriazone and butyl methoxydibenzoylmethane for the concomitant protection of skin bacteria of the genus Lactobacillus such as e.g., Lactobacillus crispatus (L. crispatus) and S. epidermidis against UV radiation.

The present invention relates to the use of selected UV filters selected from the group consisting of octocrylene, ethyl hexyltriazone and butyl methoxydibenzoylmethane for the protection of skin bacteria of the genus Lactobacillus such as e.g., Lactobacillus crispatus (L. crispatus), preferably in the concomitant presence of S. Epidermidis against UV radiation to foster the maintenance of a healthy skin microbiome upon UV irradiation.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus crispatus and application thereof in female genital tract health. The preservation number of the strain is CCTCC NO: M20231720, and the strain is excellent in antibacterial ability and vaginal mucosa adhesion ability, can effectively generate hydrogen peroxide, has no potential safety problem, and plays an important role in regulating genital tract micro-ecological balance and preventing and treating exogenous pathogenic bacterium infection.

The present invention relates to the cosmetic, nutraceutical use of a strain of Lactobacillus crispatus, in particular that filed under number CNCM I-5579, and / or of a composition comprising it, to reduce pigmentation of the skin and / or mucous membranes and / or prevent its increase, in particular to preserve and / or improve the complexion of the skin and / or mucous membranes, preferably to preserve and / or improve the radiance and / or luminosity and / or homogeneity of the complexion of the skin and / or mucous membranes, and / or to prevent the appearance and / or reduce the presence of pigment spots on the skin and / or mucous membranes and / or detoxify the skin and / or mucous membranes.It also concerns the strain of Lactobacillus crispatus, or a dermatological composition comprising it, for its use, advantageously by topical route, for the treatment and / or prevention of pathologies of the skin and / or mucous membranes associated with an increase in pigmentation of the skin and / or mucous membranes, in particular to depigment and / or prevent hyperpigmentation of pathological skin and / or pathological mucous membranes.

The invention discloses an application of a bacterial strain in production of an external product for improving the micro-ecology of the vagina of a human body, and the bacterial strain is Lactobacillus crispatus SPR003 and has 16S rDNA (ribosomal deoxyribonucleic acid) as shown in SEQ ID NO.1. The invention further discloses a preparation method of the bacterial strain for improving the micro-ecology of the vagina of the human body. The preservation number is GDMCC NO: 66664; the strain produces H2O2 and only produces D-lactic acid, and no drug-resistant gene is found; the external product is suitable for improving changes, caused by vaginal pathogenic bacteria, of micro-ecology of the vagina of the human body. Compared with other lactobacillus crispatus of the same type, the strain has the advantages that the H2O2 production capacity is high, the H2O2 production speed is high, the total amount of H2O2 produced within 12 hours is large, and the concentration of hydrogen peroxide can reach about 220 mu M / L after the strain is cultured for 12 hours in the conventional inoculation amount of 1%. H2O2 is a strong oxidant, and can directly kill or inhibit a plurality of pathogenic bacteria and conditioned pathogenic bacteria such as Gardner bacteria, campylobacter actinomycetes, prevotella and other BV-related anaerobic bacteria.