9 results about "Vaginal epithelium" patented technology

The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof, and relates to the technical field of medicines.The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof.Firstly, a mixed cell group of the vaginal epithelium containing a complete cell lineage and capable of long-term subculture is successfully established, a technical bottleneck that primary vaginal epithelial cells are difficult to expand is solved, and ideal seed cells are provided for model construction.Secondly, a highly-bionic vaginal mucosa model having a tight connection structure, rich layers and an orderly arrangement is constructed, and the physiological barrier and immune regulation functions of the vaginal mucosa are truly simulated.Finally, by introducing lactobacillus symbiosis and confirming the response to estrogen, a multi-element in-vitro research model integrating the anatomical structure of the vagina, resident flora, local immunity and endocrine regulation is constructed, and a reliable technical platform is provided for in-depth exploration of the mechanism of vaginal micro-ecological disorder and efficient screening of related drugs.

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition for treating vagina inflammation and a preparation method and application thereof, the preparation method comprises the following steps: S1, preparing a prepared rehmannia root extract; s2, preparing a salted golden cypress extract and a salted rhizoma anemarrhenae extract; s3, preparing a radix paeoniae alba extract; s4, crushing tortoise-shell glue to obtain tortoise-shell glue powder; and S5, uniformly mixing the prepared rehmannia root extract, the salted golden cypress and rhizoma anemarrhenae extract, the white peony root extract and the tortoise-shell glue powder to obtain the traditional Chinese medicine composition. The traditional Chinese medicine composition prepared by a specific method on the basis of a formula of Guanhuang mother granules has a remarkable effect of improving vagina inflammation, and all the components have a synergistic effect, so that the vagina environment is improved, vagina epithelium repair is promoted, the local resistance of the vagina is enhanced, and the effect is remarkable.

The utility model relates to the technical field of medical teaching appliances, in particular to an examination and suture teaching model capable of simulating perineum lacerations of different degrees, which comprises a model main body with a genital-imitating structure, the model main body is provided with incisions reflecting at least two degrees of lacerations, the incisions are sequentially marked as I-degree laceration and II-degree laceration, and the incisions are respectively marked as I-degree laceration and II-degree laceration. The vagina-imitating structure comprises a vagina epithelium, a perineum epithelium and a perineum muscle which are arranged in sequence; wherein the incision is obtained by cracking the vaginal epithelium to the perineal muscle by the vaginal-imitating structure. According to the scheme, an efficient platform integrating foundation and advanced suture training is provided for trainees through an innovative multi-level anatomical structure and laceration design of different depths. Students can clearly identify and practice examination and suture skills of various perineum lacerations, so that obstetrical lacerations of different degrees can be better dealt with in actual clinic, and a higher clinical skill level is achieved.

The invention provides a construction method of a human vaginal epithelium organ and application of the human vaginal epithelium organ in construction of a chlamydia trachomatis infection model, and belongs to the technical field of biomedicine. The invention provides a liquid culture medium which takes a human cervical organ culture medium as a basic culture medium and further comprises Wnt3a. Vaginal epithelial cells are inoculated into the liquid culture medium for culture, and human vaginal epithelial organs (VEOs) can be obtained. The liquid culture medium provided by the invention can support long-term amplification of vaginal epithelial cells for more than 6 months. Moreover, the vaginal epithelial cells obtained by amplification can still maintain the multiplication capacity after being subjected to long-term freeze-thaw cycle, and show excellent stability.

The invention discloses a female vaginal sensitivity regulator based on stem cells and a preparation method thereof, and belongs to the technical field of biology, the female vaginal sensitivity regulator comprises mesenchymal stem cells, tomaroside A, glycerol, a scutellaria baicalensis extract and a stem cell carrier, and a vaginal injection ointment is obtained by uniformly mixing the mesenchymal stem cells, the tomaroside A, the glycerol, the scutellaria baicalensis extract and the stem cell carrier through a proper amount of deionized water. The mesenchymal stem cells added in the application can be colonized in the vaginal epithelium and vaginal environment to promote differentiation into functional cells and secrete EGF, VEGF and other regenerative factors, collagen synthesis and angiogenesis are further promoted, the thickness of atrophic mucosa is increased, glycogen secretion and lactic acid bacteria microecology are recovered, the pH acid environment is maintained, and the problem of dryness is solved. Through the synergistic effect of the tomato steroid glycoside A and the radix scutellariae extract, the differentiation expression of the mesenchymal stem cells can be promoted while local inflammatory factors are inhibited. The low-molecular-weight lycium barbarum polysaccharide provides osmotic pressure protection, so that the survival rate of stem cells is

The invention relates to a composition for improving vaginal dryness and relaxation and a preparation method thereof, the composition comprises a fusion protein as shown in SEQ ID NO: 4, the fusion protein RGD-Collagen-FGF2 is formed by connecting an RGD element (SEQ ID NO: 1), a human III-type collagen active fragment (SEQ ID NO: 2) and an FGF2 active fragment (SEQ ID NO: 3) through a linker to form a'retention-repair-regeneration 'synergistic network effect: RGD realizes positioning retention, Collagen constructs an ECM (extracellular matrix) scaffold, and the fusion protein is used for improving vaginal dryness and relaxation. The FGF2 activates epithelial regeneration; in a vaginal dry mouse model, local administration of the fusion protein can significantly improve vaginal epithelial thickness, ECM water content and mucoprotein expression and reduce epithelial permeability, the effect is better than that of estriol positive drugs, mucosal atrophy is reversed from a pathological mechanism for the first time, and hormone dependence risks are avoided.

This invention belongs to the field of microbial technology, specifically relating to the multifunctional Lactobacillus curvatureii and its application in the preparation of health products. The Lactobacillus curvatureii (… Lactobacillus crispatus Lactobacillus crispatus The accession number for MCOlact00102 is CGMCC No. 34381. The described *Lactobacillus curvatureensis* (MCOlact00102) is... Lactobacillus crispatus ​ The two strains, MCOlact00126 (CGMCC No. 34382), exhibit significant inhibitory effects against bacterial vaginosis (BV) and infections caused by Gardnerella vaginalis, Group B Streptococcus (GBS), Escherichia coli, or Candida albicans. The safety of these strains was evaluated using a 3D vaginal epithelial organoid model, showing no irritant effect on cells. Furthermore, hemolytic activity tests, antibiotic resistance gene detection, and functional validation all demonstrated their good safety profile. In addition, the aerobic acclimation process significantly improved the stability and bioactivity of these two strains in actual production and clinical applications, laying a solid foundation for their industrialization and application.

An intravaginal electrical stimulation device for treating pelvic pain in a female patient includes, in one embodiment, a set of intravaginal components comprising a frame, at least one pair of paracervical electrodes, an intravaginal capsule, a socket in the proximal end of the intravaginal capsule, an electrode plug that plugs into the socket, and connecting wires that electrically couple the electrode plug to the paracervical electrodes. The intravaginal components are designed to inserted into the patient's vagina so that one or more electrodes are in direct contact with the vaginal epithelium in the lateral vaginal fornices. In some embodiments, a cutaneous electrode may be included to be attached to the skin of the female patient. A microprocessor and an electrical stimulation generator are operable to cause low-voltage electrical current to flow through the intravaginal electrodes, the cutaneous electrode, or both, to create one or more electrical fields that neuromodulate the intrapelvic nerves of the patient. An external controller, which communicates with the intravaginal components over a wireless data communications channel, sends instructions to and receives status updates from the microprocessor inside the intravaginal capsule. In some embodiments, the electrical stimulation generator is located inside an external electrical stimulator generator (EESG) worn outside the body, instead of being located in an intravaginally worn intravaginal capsule, and the EESG is electrically coupled to the intravaginal components via one or more connecting wires that pass through the orifice of the female patient's vagina.

The invention relates to the technical field of vaginitis medicines, in particular to an estrogen vagina foaming agent and a preparation method thereof. The estrogen vaginal foaming agent is prepared from the following components in parts by weight: 0.1 part of estrogen, 6 to 10 parts of a foam stabilizer, 180 to 220 parts of poloxamer, 100 to 120 parts of tetrafluoroethane, 8 to 12 parts of a pH buffer agent, 100 to 150 parts of medium chain triglyceride, 1 part of d-limonene, 6 parts of N-acetylcysteine and the balance of water, wherein the total parts of the estrogen, the foam stabilizer, the poloxamer, the tetrafluoroethane, the pH buffer agent, the medium chain triglyceride, the d-limonene and the N-acetylcysteine are supplemented to 1000 parts. And the foam stabilizer is chitosan or sodium hyaluronate. The estrogen is prepared into the foaming agent, so that the contact area is increased, and the treatment effect is further enhanced; by adding chitosan, bubbles can be prevented from being combined and broken due to electrostatic repulsion, and the retention time of the bubbles is prolonged; sodium hyaluronate is added, so that a physical barrier can be formed among bubbles, vaginal epithelium repair is promoted, and the treatment effect is enhanced.