6 results about "African Green Monkey" patented technology

This invention discloses a cell line capable of inhibiting African swine fever virus (ASFV) replication and its construction method, relating to the field of biotechnology. The construction method includes the following steps: knocking out the ANXA1 gene in African green monkey kidney cells to construct a stably inherited ANXA1 gene knockout cell line; this ANXA1 gene knockout cell line is the cell line capable of inhibiting ASFV replication. The cell line provided by this invention can significantly inhibit ASFV replication. Compared to existing porcine cell lines, this cell line has a clear background, is free from endogenous porcine pathogen contamination, and can be passaged indefinitely, solving the problems of cumbersome porcine cell preparation and large batch-to-batch variations. Simultaneously, this cell line provides a targeted cell model for studying the replication mechanism of ASFV and establishes an efficient evaluation system for high-throughput screening of anti-ASFV drugs, thereby facilitating the development of research related to ASFV prevention and control.

The present invention relates to a sVERO 7C2 cell line, which is a Vero cell line derived from WHO distributed Vero cells (African green monkey kidney cell line) and capable of being cultured in suspension without serum components. In addition, the present invention relates to a culture method for culturing Vero cells and a method for preparing a virus for vaccine using the Vero cells.

The application discloses an African green monkey kidney cell clone and application thereof in detection of chicken newcastle disease virus, and particularly relates to the technical field of biological product detection. The detection method specifically comprises the following steps: (1) cell plating: taking prepared VERO-K6 cell suspension, taking a certain amount of the cell suspension into a dish, and then adding the cell suspension into a cell culture plate by using a cell plating gun; the cell culture plate is placed into a culture box and cultured until the cells grow into a dense monolayer; (2) sample dilution: taking a certain amount of chicken newcastle disease virus liquid into a 10 ‑1 EP tube, oscillating and uniformly mixing the virus liquid, taking an equal amount of virus liquid from a 10 ‑1 centrifugal tube into a 10 ‑2 centrifugal tube, and sequentially performing 10 times dilution; (3) sample adding; and (4) culture: placing the cell culture plate into the culture box, culturing, recording the number of effective lesion holes, and calculating TCID 50 according to the Reed-Muench method. Compared with the existing chicken newcastle disease virus detection method, the application has the advantages of low detection cost, simple detection process, short detection period and the like.

This invention relates to a reverse genetics operating system for the segmented cloning and rescue of the Bartha K61 pseudorabies virus vaccine strain genome, its construction method, and its application. The system includes: recombinant plasmids BAC-cm-PRV-EA, BAC-cm-PRV-BC, and BAC-cm-PRV-FG containing the complete genome of the Bartha K61 pseudorabies virus vaccine strain, and host cells expressing the complete genome of the Bartha K61 pseudorabies virus vaccine strain. The reverse genetics operating system for the Bartha K61 pseudorabies virus vaccine strain of this invention successfully segments the Bartha K61 genome into six segments using ExoCET cloning and assembly technology, and then assembles them into three recombinant plasmids: BAC-cm-PRV-EA, BAC-cm-PRV-BC, and BAC-cm-PRV-FG. These three recombinant plasmids, after PacI linearization, can be co-transfected into African green monkey kidney cells to rescue the Bartha K61 virus, and the infectivity of the rescued Bartha K61 virus is comparable to that of the vaccine strain. Therefore, this reverse genetics operating system can provide an excellent vector for creating live vector vaccines related to the prevention and treatment of swine diseases, and can be used to develop Bartha K61 recombinant genetic engineering vector vaccines that express relevant swine disease antigen genes.

The application discloses application of icariside II in the technical field of traditional Chinese medicine pharmacy in preparation of a medicine for resisting coxsackieviruses group B3 (CVB3). The present application takes VERO (African green monkey kidney) and Hela (HeLa) cells infected by CVB3 as research models, detects the influence of icariside II on cell cytopathic effect (CPE) after infection by CVB3, on virus titer in supernatant, and on transcription level of CVB3 mRNA, and the results show that icariside II has obvious anti-CVB3 effect and can be used for preparation of a medicine for resisting CVB3.

This invention relates to a method and application of the inhibitor EHop-016 for inhibiting porcine epidemic diarrhea virus (PEDV) infection in vitro. EHop-016 can be used to prepare antiviral drugs for inhibiting PEDV infection. In this invention, during the in vitro infection of African green monkey kidney cells (Vero) with PEDV, the addition of 4 μmol / L EHop-016 significantly reduced the viral load, protein expression level, RNA content, and viral titer of PEDV in the EHop-016-treated group compared to the control group without EHop-016, indicating that this inhibitor can be used to inhibit porcine epidemic diarrhea virus infection in vitro.