Porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine and preparation method thereof

A technology for porcine epidemic diarrhea and epidemic diarrhea, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, and the digestive system, and can solve problems such as difficulty in ensuring consistent cell quality, high production costs, difficulty in vaccine production, and efficacy

Active Publication Date: 2013-04-17
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For domestic porcine transmissible gastroenteritis and porcine epidemic diarrhea dual live vaccines, the cell matrix for virus culture is mostly primary cells, and primary cells are composed of various types of cells. The cell types are more complex and the characteristics of cells are different. , there are differences among

Method used

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  • Porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine and preparation method thereof
  • Porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine and preparation method thereof
  • Porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine and preparation method thereof

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Experimental program
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Embodiment Construction

[0013] 1. Source of virus

[0014] Feces, duodenum, jejunum and mesenteric lymph node tissues were collected from piglets suspected of having transmissible gastroenteritis (TGE) and porcine epidemic diarrhea (PED). A suspected TGE and PED virus was isolated by the method of cell subculture, and the RT-PCR and virus neutralization test were positive. Other physical and chemical properties and biological properties were in line with the characteristics of TGEV and PEDV, and they were named SD strain and LW strain respectively. Subsequent cell passage attenuation, safety and immunogenicity tests of the two viruses proved that the two viruses had been attenuated and had good immunogenicity, and could be named as porcine infectious gastroenteritis respectively as vaccine strains. SD / L strain of Transmissible gastroenteritis virus and LW / L strain of Porcine epidemic diarrhea virus.

[0015] The SD / L strain of porcine transmissible gastroenteritis virus (Transmissible gastroenteriti...

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Abstract

The invention relates to a porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine and a preparation method of the porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine. The porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine is prepared by performing virus amplification on a swine testicular cell line (ST cells) or an African green monkey kidney cell line (Vero cells) by using a self-attenuated and preserved transmissible gastroenteritis virus SD/L strain and a self-attenuated and preserved porcine epidemic diarrhea virus LW/L strain, and carrying out the steps of harvesting, uniformly mixing, freeze-drying and the like. The porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine can effectively prevent two diseases namely swine transmissible gastroenteritis and epidemic diarrhea.

Description

technical field [0001] The invention relates to a porcine transmissible gastroenteritis and epidemic diarrhea dual live vaccine, which belongs to the field of veterinary biological products. Background technique [0002] Porcine transmissible gastroenteritis is a highly contagious, fatal infectious disease of pigs caused by Transmissible gastroenteritis virus (TGEV), which causes vomiting, severe diarrhea and dehydration in piglets under 2 weeks of age as the main feature. Among them, the mortality rate of piglets within 2 weeks of age can reach 100%, and the mortality rate of pigs over 5 weeks of age is relatively low. Larger or adult pigs hardly die, but it can cause fat loss, reduce feed remuneration, increase medicine and manpower expenses, etc. , causing serious losses. The disease has been widely prevalent in the United States since 1933. The pathogen of the disease was determined by Doyle et al. in 1946. After that, many countries and regions in the world (Japan, Br...

Claims

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Application Information

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IPC IPC(8): A61K39/225A61K39/215A61P31/14A61P1/00A61P1/12
Inventor 张淑刚张中波张青婵徐龙涛王蕾鲍海忠李一经
Owner QILU ANIMAL HEALTH PROD
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