25results about How to "Support growth" patented technology

The invention relates to the technical field of agricultural engineering, in particular to a reclamation land soilless culture and green manure intercropping culture system which comprises a reclamation land matrix preparation module, a soilless culture module, a green manure planting module and a microorganism bionic regulation and control module. The reclamation land matrix preparation module is used for preparing a matrix for soilless culture of a reclamation land; the soilless culture module comprises a culture bed, a nutrient solution supply unit and an environment control unit; the green manure planting module is provided with a special area for planting green manure crops, and the improvement of a soil structure and the optimization of nutrient circulation are realized by alternately or simultaneously planting the green manure and the crops; and the microbial bionic regulation and control module monitors and regulates and controls a microbial community structure in the matrix of the reclamation land in real time. According to the invention, not only are the ecological balance and stability of microbial communities improved, but also the nutrient absorption and disease resistance of crops are enhanced, and the growth conditions and yield of the crops are optimized.

The present invention relates to the technical field of ecological concrete slurry, and specifically to an ecological seed-carrying slurry for the slope of a mine dump, a preparation method and its application. Aiming at the special environmental characteristics of the Inner Mongolia mining area, the present invention prepares a new type of artificial aggregate containing growth domains and seeds, which is composed of a three-layer structure, each layer having a specific function, so as to achieve the purpose of promoting the germination of multi-source species, plant growth, providing nutrition and improving the soil environment. The slurry can not only effectively utilize solid waste and reduce costs, but also adopts in-situ point-type layered hole sowing to shorten the vegetation restoration cycle in the ecological restoration of the slope, greatly improve the survival rate of tree and shrub seed sources, reduce the number of watering times, save restoration costs, and reduce the workload of management. While effectively promoting vegetation restoration, it strengthens the stability of the slope, prevents soil and water loss, slows down runoff and consolidates the soil, providing an efficient and sustainable solution for the ecological restoration of green mines in Inner Mongolia.

The invention relates to the technical field of yak milk powder, and discloses yak milk powder helpful for promoting bone growth and development. Comprising the following specific raw materials: basic raw materials: raw yak milk, dried skim milk, cattle spleen peptide powder, colostrum powder, hydrolyzed yolk powder, phosphatidylserine, N-acetylneuraminic acid, colostrum basic protein, lactoferrin, docosahexaenoic acid, arachidonic acid, xanthophyll and choline; the polypeptide combination comprises Chinese yam peptide powder, poria cocos peptide powder, hawthorn peptide powder, endothelium corneum gigeriae galli peptide powder, walnut peptide and yak bone peptide. Through polypeptide combination of colostrum basic protein and composite mineral supplement, digestion and absorption of children to yak milk powder are promoted, various mineral substances needed by the body are supplemented, immunity is enhanced, bone growth is promoted, multivitamins needed by the body are supplemented by multivitamins, growth and development are promoted, bone growth is indirectly supported, and the yak milk powder has the effects of improving the immunity and improving the immunity. The requirements of children on the key nutrients in the bone growth and development stage are met.

The invention belongs to the technical field of immune cell culture, and particularly relates to an in-vitro amplification culture method of NK cells. The amplification method comprises the steps of CD16 pretreatment, plasma inactivation, lymphocyte separation and multi-stage fluid infusion. The preparation method comprises the following steps: pretreating CD16 in a T75 culture flask, promoting attachment and activation of NK cells, simulating a natural activation state, improving proliferation efficiency, treating whole blood and inactivating plasma to eliminate immunosuppressive factors, reserving nutritional ingredients, supporting amplification of the NK cells, enriching the NK cells through a lymphocyte separating medium, removing impurities and improving purity, mixing an RPMI-1640 culture medium with a solution A, and culturing for 12-24 hours at a high temperature; oK432 and IL-15 are added, the microenvironment is optimized, growth of the NK cells is remarkably promoted, liquid supplementing operation and bottling and bagging are carried out for multiple times, sufficient space and nutrition are provided, the cells are gradually transferred into a serum-free culture medium, and the cell safety and clinical application potential are improved.

The invention belongs to the technical field of aquaculture, and particularly relates to a breeding system for combined breeding of zooplankton and phytoplankton and a method for combined breeding of zooplankton and phytoplankton. By means of the breeding system, the phytoplankton releases oxygen and generates organic matter through photosynthesis, and abundant bait is provided for the zooplankton; the zooplankton is fed by the phytoplankton, nutritive salt in the generated waste is dissolved in water and becomes a nutrition source of the phytoplankton, high-density growth of the zooplankton and the phytoplankton is achieved in a limited breeding space, and land resources are effectively saved; and in combination with an intelligent monitoring system, water body environment parameters can be monitored and adjusted in real time, plankton is ensured to grow under the most suitable environment condition, the feed conversion efficiency is improved, and water body pollution is effectively reduced. The primer aquatic bacillus can increase dissolved oxygen in a water body, promote decomposition of organic matters in the water body and release of inorganic nutritive salt, and further support growth of phytoplankton.

The invention belongs to the technical field of biomedical materials, and particularly relates to a carboxymethyl chitin composite material for an articular cavity and a preparation method of the carboxymethyl chitin composite material. The carboxymethyl chitin composite material for the articular cavity is prepared by selecting carboxymethyl chitin, hyaluronic acid, sodium alginate and chitosan which are good in biocompatibility as raw materials and performing functional modification treatment respectively, and through the synergistic effect of all the components, the elasticity modulus of the composite material is effectively improved, the swelling rate is reduced, and the joint cavity is more stable. And meanwhile, the composite material also has proper viscosity and degradation performance, and can meet application requirements.

The invention discloses a method for directly inducing generation of organoids from IPS and application, and belongs to the field of cell engineering. The invention provides a method for directly and rapidly generating a target organ based on human IPS, and the lung organ, the placenta organ and the blood vessel organ are obtained. According to the method, the organoid represented by three germ layers is induced, so that the method is suitable for constructing various organoid. Compared with the prior art, the method has the advantages that step-by-step induction is not needed, one-step construction is successful, the operation is simple, the construction time is greatly shortened, the process is repeatable, long-term stable culture and passage are realized under in-vitro conditions, and the freezing survival rate is high; the method has the advantages that the method is simple in operation, the cultured organoid is good in homogeneity, RNA-seq results show that high expression of the blood vessel related genes appears in the organoid, and the maximum length of the organoid can reach 400 micrometers, so that the culture method can spontaneously induce expression of part of the blood vessel related genes to support growth of the organoid. The invention provides an important tool for researching pathogenesis of diseases and subsequent drug development.

The invention relates to the technical field of water treatment agents, in particular to a biological conditioner with water quality purification capacity and a preparation method and application thereof, and the biological conditioner is composed of a modified porous aluminosilicate inorganic material, an active microbial agent, fermentation liquor of the active microbial agent and an embedding material. The modified porous aluminosilicate inorganic material is diatomite, modified zeolite or perlite; the active microbial agent comprises bacillus, photosynthetic bacteria, alcaligenes faecalis, pseudomonas and nitrifying bacteria. The biological conditioner contains multiple beneficial microorganisms such as photosynthetic bacteria, nitrifying bacteria and bacillus, the microorganisms and multiple enzymes generated by the microorganisms have a synergistic effect, the respective purification effect can be achieved in a water body, harmful substances in the water body are synergistically degraded, and the self-purification capacity of the water body is improved.

The invention relates to the technical field of fertilizers, discloses a multifunctional soil conditioner for improving saline-alkali soil as well as a preparation method and application of the multifunctional soil conditioner, and aims at solving the problem that a traditional soil conditioner is single in function. The invention provides a comprehensive soil improvement scheme capable of simultaneously improving soil fertility, structure and microbial environment and supplementing trace elements. The multifunctional soil conditioner for improving the saline-alkali soil has remarkable effects in the aspects of improving soil quality, promoting plant growth and increasing crop yield.

The application discloses a financial risk intelligent risk control middle platform system and method based on multi-dimensional data integration, and belongs to the field of financial risk intelligent risk control. The financial risk intelligent risk control middle platform system based on multi-dimensional data integration comprises a data processing module, a model construction module, a decision construction module and a scheme formulation module. The application solves the problems of poor system integration, high maintenance cost, low availability, insufficient expansibility, low information transparency and great management difficulty in the prior art, greatly shortens the strategy iteration cycle, meets the demand of high-concurrency business scenarios, improves the accuracy of fraud identification and credit evaluation, reduces the misjudgment rate, can adapt to market changes, reduces repeated development through unified variable management, improves the data asset reuse rate, improves business autonomy, flexibly outputs the risk control capability to support personalized product design, and realizes the best balance between risk and benefit through the deep cooperation of risk control and business.

The invention provides an ecological restoration method for moss crust in a dry-hot valley area by taking slag as a main material. An ecological restoration base material comprises the following components: slag, planting soil, plant straw, a water-retaining agent, a fertilizer-retaining agent, a structure modifier, a growth promoting agent and moss powder. The slag is used as a main material, engineering waste is effectively converted into ecological restoration resources, environmental pollution and resource waste are reduced, the physical structure of soil is improved through the water-retaining agent and other admixtures, the air permeability and drainage of the soil are improved, growth of moss roots is promoted, ecological restoration is promoted, and increase of biological diversity is promoted; the formation of the moss crust can improve the local microclimate, increase the soil humidity and promote the growth of other plants, and the physical characteristics of the slag and the growth of the moss are combined, so that the moss can better survive under a drought condition, the adaptability of the moss to a dry and hot environment is enhanced, soil wind erosion is effectively prevented, and soil resources are protected.

The invention belongs to the technical field of crop fertilization, and particularly relates to a triangular fertilization method for sugar cane. Sugarcane is subjected to triangular fertilization, the sugarcane is divided into newly-planted sugarcane and perennial root sugarcane, triangular fertilization is carried out on the newly-planted sugarcane, top corner sugarcane ditch fertilization is carried out when the newly-planted sugarcane is planted, the fertilization depth is 5-10 cm, bottom corner fertilization is carried out along the middle position of a sugarcane row in the tillering stage of the sugarcane, and the fertilization depth is 30-35 cm; triangular fertilization is carried out on the perennial root sugarcanes, one-time triangular fertilization is carried out within 7 days after the sugarcanes are harvested or at the tillering stage of the perennial root sugarcanes, the fertilization depth of the 1 / 2 position of each sugarcane row is 30-35 cm, and the fertilization depth of the 1 / 4 position and the 3 / 4 position of each sugarcane row is 15-20 cm. The precise soil layer fertilization method provided by the invention has the advantages that the fertilization of sugar cane is more efficient and more environment-friendly, and farmers are helped to increase income.

The invention relates to the technical field of microbial culture, and particularly discloses a culture medium for efficiently promoting clostridium growth and a preparation method thereof. Comprising the following raw materials in percentage by mass: 13-30% of peptone, 10-25% of bovine brain extract powder, 15-26% of bovine heart extract powder, 8-18% of yeast extract powder, 1-4% of L-cysteine, 2-6% of fructose, 2-6% of glucose, 5-14% of sodium chloride, 3-7% of disodium hydrogen phosphate and the balance of thermally inactivated probiotics, the heat-inactivated probiotics comprise 0.5-4% of extracellular polysaccharide of one or more of lactobacillus, lactococcus and pediococcus or bacteria, the pH value of the culture medium is 5.0 + / -0.5, the culture medium is specifically added with yeast extract powder, L-cysteine and fructose, and under the anaerobic culture condition, the pH value of the culture medium is 5.0 + / -0.5. On the basis of conventional fermentation culture, thermal inactivation specific probiotics are added into a culture medium, so that clostridium thalli with a probiotic effect can be promoted to be greatly increased, and the logarithmic growth period of the thalli is shortened.

The invention relates to a soil remediation and water retention microbial fertilizer, which is prepared by culturing a compound strain and optimizing nutrient substances in a culture dish, preparing the most suitable growth environment of the mixed strain, increasing the effective viable count of a microbial agent, and mixing the mixed agent with organic and inorganic fertilizers to form the compound microbial fertilizer with good water retention.

The present invention provides a pharmaceutical composition comprising probiotics and prebiotics for preventing and / or relieving symptoms associated with infections obtained by extended spectrum beta-lactamase (ESBL)-producing organisms and other drug resistant pathogens. More specifically, the pharmaceutical composition comprises at least one Lactobacillus species as a probiotic and at least one oligosaccharide as a prebiotic. The invention further provides a method of preventing and / or treating infections resulting from extended spectrum beta-lactamase (ESBL)-producing bacteria and other drug resistant pathogens, comprising administering to a subject in need thereof an effective amount of a pharmaceutical composition comprising a probiotic and a prebiotic.

The invention relates to the technical field of biomedical materials and application thereof in tissue engineering, in particular to injectable piezoelectric hydrogel and a preparation method thereof.The hydrogel comprises polyvinyl alcohol, polyacrylic acid, polydopamine and I-type collagen and further comprises sodium hydroxide, a cross-linking agent, a catalyst and an initiator, finally, the injectable polyvinyl alcohol / polyacrylic acid / polydopamine hydrogel is obtained, and the postoperative rehabilitation effect of anterior cruciate ligament reconstruction can be remarkably improved.

The invention belongs to the technical field of ecological slope protection, and particularly relates to a soil fixation flood control ecological regulation and storage slope protection. Comprising a plurality of slope protection brick units which are inserted up and down and clamped left and right; each slope protection brick unit comprises a slope protection brick and a water absorption and guide block, and the slope protection brick is higher than the water absorption and guide block; conical piles are inserted into the slope protection bricks, and the lower ends of the conical piles are inserted into the slope. The slope protection brick is good in anti-sliding capacity, a self-locking structure is formed between the slope protection brick units, stability is kept in the transverse direction and the longitudinal direction, and a slope body is more stable; in addition, the protection slope can bear broken stones, mud scraps or rainfall runoff which roll down from the slope top, the broken stones, mud scraps or rainfall runoff are accumulated in the protection slope brick units, and the landslide risk is reduced. The protection slope has better landscape and ecological effects, water can be stored step by step, the stored water and nutrients carried by the charcoal matrix are utilized, and growth and maintenance of vegetation and an ecological system in a region are supported.

The invention discloses a method for three-dimensionally culturing cells by using a colloid for promoting cell growth and mass production of a high-concentration extracellular composition by using the method. The method for three-dimensionally culturing cells by using the colloid for promoting cell growth comprises the following steps: providing a culture colloid to mix and coat the cells; performing cross-linking reaction on the culture colloid coated with the cells, so that the cross-linked culture colloid presents a three-dimensional structure, and the cells are suspended in the cross-linked culture colloid; and carrying out cell culture. Through the technical content of the invention, the damage of the cells in the three-dimensional culture process can be reduced, and the three-dimensional growth of the cells in the colloid is supported.

The invention relates to a 4D printed bamboo fiber lumen stent and a preparation method thereof, and belongs to the technical field of medical instruments. In order to solve the problems that an existing shape memory lumen stent is poor in mechanical durability and the interfacial compatibility of natural fibers and shape memory polymers is poor, the 4D printing bamboo fiber lumen stent is provided and is prepared from continuous bamboo fibers and the shape memory polymers, and the volume fraction of the continuous bamboo fibers is 15-60%. According to the method, the interface performance of the continuous bamboo fibers and the shape memory polymer is optimized through instantaneous heat treatment, and the mechanical durability of the lumen stent is remarkably improved. Due to the shape memory characteristic, the stent can be tightly attached to the inner wall of a lumen and can better adapt to a complex physiological environment, and stent displacement risks and complications are reduced. The composite coating is prepared on the surface of the lumen stent, so that the biocompatibility and the antibacterial property of the lumen stent are further improved, and an innovative and effective solution is provided for clinical treatment of diseases such as lumen stenosis or fistulous tracts.

The invention discloses a calf feed additive and a preparation method thereof. The feed additive is prepared from the following components: 100 parts of Chinese herbal medicine fermentation liquor, 10-30 parts of modified hydrogel, 8-16 parts of alfalfa meal, 5-10 parts of calcium hydrogen phosphate, 5-10 parts of whey powder, 1-3 parts of choline chloride, 0.5-2 parts of table salt and 0.1-1 part of baking soda. The Chinese herbal medicine fermentation liquor is prepared by fermenting a Chinese herbal medicine extracting solution with a complex microbial inoculant, and the modified hydrogel is prepared by crosslinking lipoic acid modified bentonite and fumaric acid modified sodium alginate under photo-initiation; the prepared feed additive is good in palatability, the feed intake of the calves can be remarkably increased, meanwhile, the gastrointestinal digestion function of the calves is enhanced, the digestion, conversion and utilization rate of the feed is increased, and rapid growth of the calves is promoted.

The invention provides a casein source 3D printing bio-ink and a preparation method of a biological scaffold and cell culture meat. The bio-ink is prepared from the following raw materials in parts by weight: 15.6 parts of gelatin; 2.6 parts of sodium alginate; 4.2 parts of casein; 52.5 parts of a sodium hydroxide deionized water solution with a pH value of 8; and 87.5 parts of a sterile 1 * PBS buffer solution. The ink is subjected to extrusion type 3D printing and calcium chloride crosslinking to obtain a biological scaffold; c2C12 cells are inoculated on the support, and cell culture meat is obtained through proliferation-differentiation culture. The problems that existing 3D printing bio-ink is single in performance and poor in component adaptability, a biological scaffold material is insufficient in mechanical stability, and nutritional ingredients are deficient are solved. The prepared bio-ink has excellent mechanical properties and printing adaptability, and provides key technical support for industrial preparation of cell culture meat.

The invention discloses a method for increasing the content of gamma-aminobutyric acid in traditional Chinese medicine through fermentation. The method comprises the following steps: S1, superfine grinding of raw materials; s2, performing enzymolysis treatment; s3, fermentation treatment; lactobacillus plantarum and lactobacillus reuteri are selected to be matched for fermentation. A compound enzyme system (cellulase, pectinase and hemicellulase) is added, and the enzyme addition amount is 600 U / g-1400 U / g; the content of GABA in a bacterium synergistic fermentation system is obviously higher than that of a single bacterium fermentation system. Experimental data show that the GABA content can reach 131.5 + / -0.7 [mu] mol / g under the synergistic fermentation condition for 48 h, the GABA content under the lactobacillus plantarum single bacterium fermentation condition and the GABA content under the lactobacillus reuteri single bacterium fermentation condition are 45.5 + / -1.1 [mu] mol / g and 29.9 + / -2.1 [mu] mol / g respectively, the GABA yield is increased by about 2.9-4.4 times through synergistic fermentation, and it is proved that the method can effectively promote efficient conversion and accumulation of glutamic acid into GABA.

The invention belongs to the technical field of biomedical materials, and particularly relates to biomimetic frog-derived nerve regeneration peptide modified bioactive hydrogel and a preparation method thereof. The bioactive hydrogel is prepared from the following raw materials: RADA16, CS, VD11 and deionized water; the PNFs hydrogel is prepared from PNFs, CS and VD11 through self-assembly of RADA16 and deionized water, and the optimal mass concentration ratio of PNFs to CS to VD11 in the prepared bioactive hydrogel is 3.33: 20: 0.135. The preparation method is simple, conditions are easy to realize, the use amount of expensive RADA16 can be reduced, and the preparation method is particularly applied to central nervous system injury repair. The bioactive hydrogel has a three-dimensional space suitable for loading stem cells or drug molecules, is used as a carrier for cell culture and stem cell transplantation, supports attachment and growth of cells and promotes tissue repair and regeneration, and has a very wide application prospect.

The invention discloses a feed capable of reducing cholesterol content in goose meat and a preparation method of the feed, and belongs to the technical field of goose feeds. Comprising the following steps: mixing hawthorn, dried fish powder, bone meal, carrot powder and kelp powder, grinding into fine powder 1 for later use, mixing wheat, rapeseed meal, oat, corn, bean cake and sorghum straw, grinding into fine powder 2 for later use, mixing the fine powder 1, the fine powder 2, glycosylated phytosterol, cod-liver oil, edible salt and multivitamins in a mixing machine, and granulating to obtain a finished product. And adding water, heating and concentrating, and granulating by using a steam granulator to obtain the feed. Through scientific and reasonable formula design, comprehensive and balanced nutrients in the feed are ensured, and the growth and health requirements of geese are met. In addition, the glycosylated phytosterol is prepared, the bioavailability of the glycosylated phytosterol is improved, and the cholesterol content in the goose meat is effectively reduced.

The present invention provides a preparation process of a probiotic powder for promoting digestion, the preparation process comprising the following steps: (1) preparing freeze-dried powder of Lactobacillus acidophilus, freeze-dried powder of Bifidobacterium animalis, freeze-dried powder of Lactobacillus plantarum, freeze-dried powder of Lactobacillus casei, and freeze-dried powder of Bifidobacterium bifidum; (2) mixing the freeze-dried powder, inulin, oligofructose, and lactitol to obtain a mixed powder; (3) adding the mixed powder to water, stirring clockwise to obtain a mixed solution; (4) concentrating the mixed solution into a soft material in a vacuum concentrator at 10-15°C, passing through a 20-40 mesh sieve to obtain wet granules; (5) adding the wet granules to a vacuum freeze dryer for vacuum freeze drying, freeze drying in the vacuum freeze dryer to obtain a dry concentrate; (6) grinding the dry concentrate to obtain a powder raw material to obtain a probiotic powder for promoting digestion. The probiotic powder of the present invention has a good digestion-promoting effect and a high degree of comfort when taken.