Non protein culture medium adapted to large-scale culture of NSO cell and production of antibody
A protein-free medium, large-scale culture technology, applied in the field of medium research and development of modern biotechnology, can solve the problems of complex components, loss, and unsuitability for large-scale culture of NSO cells and antibody production.
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[0096] The present invention is specifically described below through examples, but the present invention is not limited to the following examples.
[0097] A protein-free medium suitable for large-scale cultivation of NSO cells and antibody production, including amino acids, vitamins, salts, lipids, trace elements, hormones, buffers and glucose, citric acid, taurine, thymidine, adenocarcinamide Purine, hypoxanthine, phenol red, mercaptoethanol, Pluronic F-68, composition without protein and hydrolyzate, the content of each component is: (mg / L)
[0098] Alanine (L-Alanine) 104.45
[0099] Arginine (L-Arginine HCI) 301.2
[0100] Asparagine (L-Asparagine.H 2 O) 202.5
[0101] Aspartic acid (L-Aspartic Acid) 106.65
[0102] Cysteine (L-Cysteine.HCI.H 2 O) 67.56
[0103] Cystine (L-Cystine.2HCI) 75.73
[0104] L-Glutamic Acid Sodium 1191.00
[0105] Glutamine (L-Glutamine) 292.00
[0106] Glycine 68.75
[0107] Histidine (L-Histidine.HCI.H 2 O) 73.48
[0108] Isoleucin...
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