Non protein culture medium adapted to large-scale culture of NSO cell and production of antibody
A protein-free medium, large-scale culture technology, applied in the field of medium research and development of modern biotechnology, can solve the problems of complex components, loss, and unsuitability for large-scale culture of NSO cells and antibody production.
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[0096] The present invention will be specifically described below through examples, but the present invention is not limited to the following examples.
[0097] A protein-free medium suitable for large-scale cultivation of NSO cells and antibody production, including amino acids, vitamins, salts, lipids, trace elements, hormones, buffers and glucose, citric acid, taurine, thymidine, and adenosine Purine, hypoxanthine, phenol red, mercaptoethanol, Pluronic F-68, a composition free of protein and hydrolysate, the content of various ingredients is: (mg / L)
[0098] L-Alanine 104.45
[0099] L-Arginine HCI 301.2
[0100] Asparagine (L-Asparagine.H 2 O) 202.5
[0101] Aspartic Acid (L-Aspartic Acid) 106.65
[0102] Cysteine (L-Cysteine.HCI.H 2 O) 67.56
[0103] Cystine (L-Cystine.2HCI) 75.73
[0104] Glutamic Acid Sodium (L-Glutamic Acid Sodium) 1191.00
[0105] L-Glutamine 292.00
[0106] Glycine 68.75
[0107] Histidine (L-Histidine.HCI.H 2 O) 73.48
[0108] Isoleucine (L-lsoleucine) ...
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