19 results about "Phenol red" patented technology

The present invention provides a visual indicator film for the freshness of fresh-cut fruits, a preparation method, the use and a detection method. The preparation method comprises the following steps: preparing a Co-based MOF; washing and activating the Co-MOF; preparing a CMC-Na-based thin film; and preparing a CMC-Na-based thin film, and thereby obtaining an indicator film for phenol red (PR) or an indicator film for bromothymol blue (BTB). In the present invention, an irregular spherical Co-based metal organic framework (Co-MOF) loaded with PR or BTB is prepared, and the nanocomposites Co-MOF / PR and Co-MOF / BTB are obtained after dyes and the MOF are adsorbed by means of the hydrogen bond interaction. Then, the nanocomposites are embedded into a sodium carboxymethyl cellulose (CMC-Na) base material to prepare CO2-responsive packaging materials, namely, a CMC-Na / PR / Co-MOF and a CMC-Na / BTB / Co-MOF, which are used for monitoring the freshness of fruits in real time and in situ. The Co-MOF-based composite thin film has good water resistance, time-temperature stability and mechanical properties, and also exhibits enhanced ultraviolet-visible light barrier properties, dye migration resistance and thermal stability. The visual indicator film for the freshness of fresh-cut fruits can effectively reflect the concentration of CO2, and is suitable for real-time and in-situ monitoring of the freshness of fruits.

The invention belongs to the technical field of biological medicine, and particularly relates to a mycoplasma synoviae culture medium capable of improving strain titer and a preparation method of the mycoplasma synoviae culture medium. The mycoplasma synoviae culture medium comprises a basic culture medium and auxiliary components, the basic culture medium is prepared from HEPES, milk protein hydrolysate, MEM, beef heart extract powder, coenzyme I, 1% phenol red, magnesium sulfate heptahydrate, L-cysteine hydrochloride, lactose-N-tetrasaccharide, disodium clodronate and water; the auxiliary components comprise porcine serum and penicillin. According to the mycoplasma synoviae culture medium obtained by the preparation method, the passage time of the mycoplasma synoviae is shortened, and the strain titer is improved. The inactivated vaccine prepared from the chicken mycoplasma synoviae cultured by the culture medium is good in safety and immunogenicity, can effectively prevent infection of the chicken mycoplasma synoviae, and has a good application prospect.

The application belongs to the technical field of biological medicine, and particularly relates to a chicken synovial mycoplasma culture medium capable of improving strain titer and a preparation method thereof. The chicken synovial mycoplasma culture medium comprises a basic culture medium and auxiliary components; the basic culture medium comprises HEPES, milk protein hydrolysate, MEM, beef heart infusion powder, coenzyme I, 1% phenol red, magnesium sulfate heptahydrate, L-cysteine hydrochloride, lacto-N-tetraose, disodium chlormethine and water; and the auxiliary components comprise pig serum and penicillin. The chicken synovial mycoplasma culture medium obtained by the preparation method shortens the passage time of chicken synovial mycoplasma and improves the strain titer. The inactivated vaccine prepared from the chicken synovial mycoplasma cultured by the culture medium has good safety and immunogenicity, can effectively prevent chicken synovial mycoplasma infection, and has a good application prospect.

The invention discloses a pharmacodynamic test method for a cough relieving mixture, which comprises the following steps: constructing four core pharmacodynamic test sub-models containing ammonia water to cause young mouse cough, citric acid to cause young guinea pig cough, rabbit in-vitro trachea cilia mucus movement and young mouse trachea phenol red excretion and a body model of a test result analysis sub-model; each pharmacodynamic test sub-model is provided with a plurality of groups of test arrays and test individuals; pathogen data is imported to generate a simulated disease scene, and a corresponding drug sequence is called to intervene and collect physiological index change data; and multi-dimensional pharmacodynamic parameters are evaluated through the analysis sub-model, and a dose-effect relation curve and an evaluation report are generated. According to the invention, a parallel verification system for antitussive and expectorant tests is formed, the antitussive effective dose, the expectorant effective dose and the effective dose range under the dual action of the medicine are determined through multi-model cross validation, the accuracy degree of judging the effectiveness of the medicine is greatly improved, and a reliable method support is provided for evaluating the efficacy of the cough-stopping mixture.

The application discloses a rapid detection method for the cleanliness of a slide for chromosome preparation for visual analysis, and relates to the technical field of chromosome detection, and comprises the following steps: step one, phenol red is added to anhydrous methanol and glacial acetic acid, the mixture is uniformly mixed, sealed and left to stand, after undissolved particles are precipitated, the supernatant clear orange liquid is taken as a detection reagent; step two, the system sampling method is used to screen and detect pathological slides in a whole box, and the same batch of pathological slides without defects and pollution are detected; step three, a plurality of pathological slides that pass the detection are selected and placed obliquely, and the detection reagent is added dropwise; and step four, the cleanliness of the pathological slides is judged. The visual rapid cleanliness detection method has the advantages of simple process, intuitive interpretation, effective satisfaction of the daily quality control demand of the slide for chromosome preparation in a laboratory and popularization value.

The invention relates to the field of microbiological detection, in particular to an LAMP (loop-mediated isothermal amplification) primer group for visual rapid detection of Brucella as well as a kit and application of the LAMP primer group. The LAMP primer group for visual rapid detection is designed aiming at a bcsp31 gene sequence and comprises a forward outer primer (F3), a reverse outer primer (B3), a forward inner primer (FIP-T), a reverse inner primer (BIP-T), a forward loop primer (LF) and a reverse loop primer (LB). When the kit is used, DNA of a sample to be detected is extracted, the primer group is added, a fluorescent dye or methylphenol red is added, an amplification reaction is carried out, and whether the sample to be detected contains brucella or not is judged according to a color development result. The invention provides an LAMP (loop-mediated isothermal amplification) primer group, a kit containing the primer group and application of the LAMP primer group, wherein the LAMP primer group takes a bcsp31 gene as a reference sequence, is shorter in response time, high in sensitivity, good in compatibility and suitable for visual detection of Brucella in daily frequent public health.

The invention discloses a visual paper-based cancer miRNA marker detection kit, the kit comprises an integrally formed foldable T-shaped filter paper, and a DNA probe TEprobe, urease and a color developing agent dropwise added on the filter paper, and the principle of the kit is as follows: the TEprobe is combined with a cancer nucleic acid marker (miRNA) to release Ag < + > to inhibit the activity of the urease; the composite signal response of the pH response probe phenol red and fluorescein is triggered, and the detection sensitivity is as low as 10-12 M. The kit provided by the invention can significantly shorten the detection time and reduce the sample demand, and realizes zero false positive rate and 97.10% specificity by combining with machine learning algorithm classification during result analysis. The performance is successfully verified in real samples of prostatic cancer, liver cancer and colorectal cancer, and a cheap and convenient means is expected to be provided for family self-inspection and cancer screening and diagnosis in resource-deficient areas.

This application discloses a serum-free cryopreservation solution for cells and its applications. The serum-free cryopreservation solution comprises: 1-2% recombinant human albumin, 5-10 μM (-)-Blebbistatin, 50-100 μM antioxidant, 5-10% permeable cryoprotectant, and a basic culture medium free of phenol red indicator. This serum-free cryopreservation solution is suitable for the cryopreservation of various cell types, particularly muscle cells, especially human induced pluripotent stem cell (iPSC) differentiated cardiomyocytes. It can significantly improve the survival rate, structural integrity, and effective preservation of the physiological functions of cardiomyocytes after resuscitation. It is applicable to basic scientific research, disease modeling, drug screening, and clinical treatment related to cardiomyocytes.

This invention belongs to the field of molecular biology technology, specifically relating to a liquid- and lyophilized dual-purpose LAMP reaction colorimetric premix, lyophilized formulation, and preparation method. The premix comprises: BST DNA polymerase, magnesium sulfate, potassium chloride, phenol red, BSA, mannitol, trehalose, and a mixture of dNTPs. This premix is ​​a completely exogenous buffer-free LAMP red-yellow colorimetric premix, achieving a seamless, abrupt red-yellow colorimetric transition, completely eliminating human interpretation errors caused by intermediate colors, and achieving 100% accuracy for non-professional interpretation. By completely eliminating buffer components, the cause of pH drift during lyophilization is eliminated at its source. Combined with a ternary synergistic protection system, the pH fluctuation before and after lyophilization is <0.1, the phenol red background remains stable over a long period, and the colorimetric window does not decay. The same formulation is suitable for both direct use in fresh liquid form and integrated lyophilization after mixing with primers, eliminating the need for separate formulation development for different scenarios.

This invention provides a composition with antitussive and expectorant effects and its preparation method. The composition comprises raw materials such as Selaginella uncinata, Smilax glabra, Ficus microcarpa, Erythrina variegata, Basil, Cinnamomum cassia, Bambusa textilis, Rehmannia glutinosa, Prunus armeniaca, Pinellia ternata, Fritillaria thunbergii, and Magnolia officinalis. This invention uses a topical patch for administration through acupoint application, which can significantly prolong the cough latency period, reduce the frequency of coughs, and increase the amount of phenol red secreted from the trachea, exhibiting significant antitussive and expectorant effects. It addresses the root cause by tonifying Qi, resolving phlegm, relieving cough, and regulating Qi, and is suitable for coughs with excessive phlegm caused by spleen deficiency and internal dampness, and heat toxicity obstructing the lungs in the Lingnan region.

Provided is a transfection reagent, comprising a solvent, organic substances, amino acids, vitamins and inorganic salts, wherein the organic substances comprise D-glucose, sodium pyruvate, i-inositol, phenol red and choline chloride; the amino acids comprise glycine, L-glutamine, L-valine, L-isoleucine, L-leucine, L-threonine, L-lysine hydrochloride, L-phenylalanine, L-serine, L-tyrosine disodium salt, L-arginine hydrochloride, L-methionine, L-cystine dihydrochloride, L-histidine hydrochloride and L-tryptophan; the vitamins comprise nicotinamide, pyridoxine hydrochloride, thiamine hydrochloride, folic acid, D-calcium pantothenate and riboflavin; and the inorganic salts comprise sodium chloride, sodium bicarbonate, potassium chloride, calcium chloride, sodium dihydrogen phosphate, magnesium sulfate, hydrochloric acid and ferric nitrate. The transfection reagent is suitable for a wide range of applications, achieves the rapid onset of transfection, and has a good transfection effect.

This invention belongs to the field of LAMP detection reagent technology, specifically relating to a fluorescence / colorimetric dual-mode LAMP detection reagent, its preparation method, and its application. The fluorescence / colorimetric dual-mode LAMP detection reagent of this invention comprises: BST DNA polymerase, magnesium sulfate, potassium chloride, phenol red, BSA, mannitol, trehalose, a mixture of dNTPs, a DNA-binding fluorescent dye, and ammonium sulfate. This fluorescence / colorimetric dual-mode LAMP detection reagent of the present invention simultaneously contains a phenol red colorimetric system and a SYBR Green I fluorescence system, allowing the same reaction tube to be read using a fluorescence instrument or observed visually. It eliminates the need to pre-select reagent types based on the detection scenario, providing the flexibility of "one tube, two modes" to adapt to more diverse detection needs. By introducing ammonium sulfate as a reaction enhancer, the different requirements of the two reading modes for the ionic environment are balanced, achieving "dual excellence" in both fluorescence signal and colorimetric effect.

The invention discloses a preparation method and application of a multifunctional chemical culture medium for mycoplasma culture and detection. The culture medium consists of 80-95 parts of a basic culture medium, 2-8 parts of a specific growth promoting factor, 0.5-3 parts of a selective inhibitor, 1-5 parts of a multifunctional indicator and 1-4 parts of trehalose. Wherein the specific growth promoting factor is a compound of arginine and glutamine, the selective inhibitor is a compound of amphotericin B and thallium acetate, and the multifunctional indicator is a compound of phenol red and triphenyltetrazole chloride. The preparation method comprises the following steps: dissolving the basic culture medium, adding the components, stirring and mixing, adjusting the pH value to 7.6-7.8, subpackaging, inflating and storing. According to the culture medium, by optimizing the synergistic effect of the components, the proliferation rate (up to 90% or above) and the detection sensitivity (the lowest detection limit is 10 CCU / mL) of the mycoplasma are remarkably improved, the positive detection time is shortened to 42-48 h, efficient culture and rapid and visual interpretation of the mycoplasma are achieved, and a reliable solution is provided for clinical diagnosis.

The invention discloses an all-fiber piezoelectric sensing film with a Janus structure as well as a preparation method and application of the all-fiber piezoelectric sensing film, and belongs to the technical field of sensors. The full-nanofiber Janus membrane with one-way liquid transmission and multi-mode sensing functions is prepared through a two-step electrostatic spinning method. The preparation method comprises the following steps: firstly, performing electrostatic spinning on a polyvinylidene fluoride-hexafluoropropylene spinning solution doped with MXene nanosheets to form a piezoelectric functional layer PM membrane; and then, by taking the PM film as a substrate, carrying out secondary electrostatic spinning on a PAN spinning solution loaded with a phenol red indicator to form a hydrophilic sensing layer PR film, and finally forming the Janus structure composite film. Efficient self-powered physical signal sensing is achieved through the high piezoelectric coefficient of the membrane, the membrane is endowed with asymmetric wettability through the Janus structure, directional management of sweat can be achieved, visual colorimetric detection of the pH value of sweat is achieved in combination with phenol red, and the membrane has wide application prospects in the fields of dynamic health monitoring, exercise rehabilitation, personalized medical treatment and the like.

The invention provides an antibacterial hemostatic gel patch and a preparation method thereof, and the method comprises the following steps: taking 3% w / v of cross-linked sodium carboxymethyl cellulose as a hydrogel matrix carrier, uniformly dispersing 2% w / v of chitosan and 0.1% w / v of polyhexamethylene biguanide in a hydrogel matrix, injecting into a mold, and freeze-drying to form inner-layer gel with a slightly convex arc-shaped surface; preparing a mixed solution containing 50% w / v of medical glycerin and 0.5% w / v of sodium hyaluronate, and carrying out film casting on the mixed solution to form a middle buffer layer; the method comprises the following steps: by taking a medical polyurethane film as a base material, coating a phenol red derivative on the inner side of the polyurethane film as a color indicator to form an outer-layer polyurethane film; the middle buffer layer and the inner-layer gel are attached to form hydrogen bond combination through hydroxyl and amino, the middle buffer layer and the outer-layer polyurethane film are bonded through medical acrylate adhesive, and the gel patch is prepared. The gel patch can realize multiple functions such as rapid hemostasis, continuous antibiosis, moisturizing and repairing, and intelligent errhysis monitoring, and is suitable for nursing of puncture points after liver cancer radiofrequency ablation.

The invention relates to the technical field of microbiological detection, and particularly discloses a culture medium for selectively separating and culturing Burkholderia cepacia, which comprises the following components: 8-12 g / L of tryptone, 8-12 g / L of sucrose, 1-2 g / L of yeast extract, 1-2 g / L of monopotassium phosphate, 5-10 g / L of sodium pyruvate, 4-7 g / L of sodium chloride, 15-23 mg / L of phenol red, 10-18 g / L of agar, 0.8-1.3 mg / L of crystal violet, 0.5-5 g / L of selective inhibitor, and the balance of water. 10-30 g / L of a specific accelerant and the balance of water; the selective inhibitor is selected from one or more of sodium deoxycholate, sodium taurocholate, sodium glycocholate and propyl gallate; the specific accelerant is lactose, cane sugar and vanillic acid; and the culture medium does not contain any antibiotic component. The culture medium disclosed by the invention is free of antibiotic components, good in selectivity and high in growth promoting ability, and has a very good market application prospect.

The invention discloses a method for detecting the content of phenol red in liquid for an in-vitro assisted reproduction technology and a verification method, and belongs to the technical field of biomedical analys.The detection method comprises the steps that a to-be-detected sample solution is pretreated, ultrapure water is used for dilution step by step according to the concentration of phenol red in the sample solution, and the sample solution suitable for analysis is obtained; the method comprises the following steps: weighing a phenol red standard substance, dissolving with ultrapure water containing ammonia water, fixing the volume, preparing into a high-concentration stock solution, accurately diluting the high-concentration stock solution to obtain a secondary stock solution, transferring different volumes of secondary stock solutions, fixing the volume with ultrapure water, preparing into a series of standard curve working solutions, measuring and analyzing the standard solution and the pretreated sample solution by LC-MS / MS, drawing a standard curve by taking the concentration of the phenol red in the standard solution as a horizontal coordinate and the corresponding chromatographic peak area as a vertical coordinate, substituting the peak area of the phenol red in the sample test solution into the standard curve, and calculating the concentration of the phenol red. And calculating the content of phenol red in the original sample according to the dilution ratio.