228 results about "Phenol red" patented technology

The invention discloses a conditioned medium preparation of mesenchymal stem cells used for skin aging restoration. A preparing method of the conditioned medium preparation includes: culturing the mesenchymal stem cells under normal conditions until the growth density of the mesenchymal stem cells is 60-80%, performing liquid changing, culturing with a phenol red-free DMEM, adding astragaloside, performing induction culturing in an incubator for 24 h, removing cytokines having a molecular weight lower than 3000 after the culturing is finished, concentrating, detecting the cytokines in the medium, adding 0.01-0.6 mg / mL of reduced glutathione and 0.01-0.4% of hyaluronic acid into the concentrated culture medium solution. The conditioned medium preparation is rich in a plurality of the cytokines. The astragaloside can maintain the characteristics of the stem cell and stimulates the capability of secreting the plurality of the cytokines of the stem cells. The hyaluronic acid avoids the problems of short half-life period of the cytokines and short curative effect time, and enhances the skin restoration effects.

The invention discloses a method for treating dye waste water by enzyme production through mixed biomass fermenting. In the method, white rot fungi which can generate a lignin degrading enzyme system are used as a fermenting enzyme-production strain; a solid and liquid culture method is adopted, a cottonseed hull and / or paddy straw are / is used as a fermentation substrate, and lignin degrading enzyme is induced to obtain an optimal lignin degrading enzyme system; and finally, crude enzyme liquid is extracted, separated and prepared and can be used for decoloring treatment of waste water of commonly-used synthetic dye, such as Congo red, phenol red, bromophenol blue, crystal violet, malachite green and the like so as to obtain the optimal decoloring effect of dye sewage. The method is simple and is easy to operate. The enzyme system induced by the method disclosed by the invention has high enzymolysis efficiency, and the optimal decoloring effect can be achieved by adopting the enzyme system to treat the dye waste water.

The invention relates to the technical field of fish detection, and particularly relates to fish freshness detection test paper. Each liter of an indicator solution in a reagent layer contains 0.5-1g of bromophenol red, 0.6-1.1g of bromcresol purple, 0.3-0.5g of bromothymol blue, 0.3-0.8g of neutral red, 0.2-0.6g of phenol red and the balance of a solvent. The fish freshness is detected rapidly with low cost and high flexibility. The detection test paper can be used for detecting the freshness of chilled, refrigerated and frozen fish. The using method is simple; and only less amount of fish body surface mucus is dipped when the test paper is used, and the fish freshness can be obtained by comparing the color instantly developed by the test paper with a standard color chart.

A kit for quickly determining infection of polyloric spirillum consists of kit body, application reagent prepared from distilled water and 1% of urase solution, plastic reaction plate with round scrobicula on its surface and Huatemen test paper prepared by soaking said paper completely in 1% of urea solution and in phenol red indicator then drying it in the air.

The present invention provides a test strip and a detecting method thereof. The test strip comprises a first sheet, a second sheet a first filter and a second filter. The first sheet comprises a bulge portion deposited substantially in the center thereof. The second sheet comprises a depression in alignment with the bulge portion to form a sealing space. The first filter is disk-shaped and deposited in the sealing space. The first filter is used to receive a biological sample. The second filter is ring shaped and disposed in the sealing space and under the first filter to receive the biological sample coming from the first filter. The first filter comprises sodium azide, sodium dihydrogen phosphate, and urea, and the second filter comprises sodium azide, phenol red, and urea.

The invention provides a humidity indicator which is really effective in environmental protection and comprises a carrier and solution used for soaking the carrier; wherein, the solution takes 30-80% of ethanol as a solvent and contains 0.00862-0.00992% of acid-base indicator and 0.8-13.8% of hygroscopic salt. The acid-base indicator is one of bromocresol green, bromothymol blue, thymol blue, thymolphthalein, bromophenol blue, methyl red, methyl orange, methyl yellow , neutral red and phenol red or the mixture thereof. The hygroscopic salt is alkali halide or alkali-earth metal halide. The humidity indicator of the invention has wide indicating range (capable of indicating 5-90% of ambient humidity) and high sensitivity. Diverse humidity indicators different in discoloration can be manufactured by adjusting the components. The humidity indicator of the invention has evident discoloration effect, simple use, reutilization, good suitability and extensive application.

The invention discloses an umbilical cord preserving fluid for temporary preservation of umbilical cord after collection before separation. The umbilical cord preserving fluid comprises the following main components: sodium chloride, glucose, human serum albumin, potassium chloride and anhydrous calcium chloride, as well as auxiliary components: cefoperazone sodium and phenol red, wherein the main components have the effects on maintaining osmotic equilibrium of inner cells of the umbilical cord tissue, keeping the umbilical cord in a wet state and providing nutrient content for a short period of time; and the auxiliary components have the effects on preventing probable pollution and prompting happened pollution. The preserving fluid can be used for preserving the activity of mesenchyme stem cells in the umbilical cord, the umbilical cord can be effectively preserved at 2-10 DEG C for at least 72 hours, the activity of the separated mesenchyme stem cells is minimally influenced by time, the time limit from collection to preparation of the umbilical cord is greatly reduced, and all the used components meet a clinical use standard and are capable of effectively reducing the pollution probability.

The invention discloses a serum-free culture medium suitable for immune cell large-scale culture, and belongs to the field of cell biology and medical immunology. The serum-free culture medium takes an RPMI1640 culture medium as a basis, additive components are further contained and include HEPES, phenol red sodium salt, L-glutamate and sodium bicarbonate. Insulin, transferrin, selenium, human albumin and interleukin-2 can be further added. Components contained in the culture medium are chemical defined components, no animal serum components are contained, and the serum-free culture medium is suitable for large-scale culture of immunity cells and is high in clinical safety and good in proliferation effect.

The invention discloses an umbilical cord mesenchymal stem cell preparation and a preparation method and application thereof. Through exploration and verification by a large quantity of experiments, the 3rd-10th-generation umbilical cord mesenchymal stem cells are selected as raw materials, and by a method of dispersing tissue by a mechanical method and then performing treatment with mixed enzymes containing collagenase I, collagenase II and trypsin for a short time for many times, the vitality of the separated umbilical cord mesenchymal stem cells is guaranteed. A culture medium special for the umbilical cord mesenchymal stem cell preparation, containing DMEM / F12 (without phenol red), 10% FBS, 20 kinds of amino acids and 8 kinds of vitamins, is adopted, mixed liquor of repeated cell culture supernatant and cell lysate supernatant is collected, more umbilical cord mesenchymal stem cell active protein and active factors are obtained, the preparation cost is reduced, and industrial requirements for a stem cell product preparation are met. The prepared umbilical cord mesenchymal stem cell preparation can promote proliferation of epidermic cells and skin fibroblast, is safe and is free from toxic and side effects, an allergy does not exist, and when the umbilical cord mesenchymal stem cell preparation is applied to feature-beautifying skin-care products, the effects are notable.

The invention discloses a mycoplasma hyopneumoniae culture medium and a preparation method thereof, which belong to the technical field of veterinary biological products. Each 1000 ml of phosphate buffer contains 20-50 g of PPLO (pleuropneumonia-like organism), 2-10 g of yeast powder, 3-15 g of glucose, 0.5-4 ml of 1% phenol red, 2-10 g of amino acid composition, 15-30 g of compound traditional Chinese medicine polysaccharide and 50-100 ml of pig serum. When culturing the mycoplasma hyopneumoniae, the mycoplasma hyopneumoniae culture medium disclosed by the invention can improve the tolerance of the mycoplasma hyopneumoniae and reduce the apoptosis rate of the mycoplasma hyopneumoniae; the mycoplasma hyopneumoniae culture medium disclosed by the invention can increase the amount of culture bacteria of the mycoplasma hyopneumoniae, the concentration of the culture bacteria liquid reaches 10<10-11> / ml, which is increased by more than 5-10 times compared with that of a common culture medium; the mycoplasma hyopneumoniae culture medium disclosed by the invention can inhibit the growth of other bacteria when culturing the mycoplasma hyopneumoniae so as to reduce the culture pollution risk of culturing the mycoplasma hyopneumoniae.

The invention provides a stem cell preparation for skin beauty and a preparation method thereof, wherein the method comprises: a) providing stem cells acquired from a mammal animal; b) subjecting the stem cells to primary culture; c) subjecting the stem cells to subculture in a stem cell medium with vitamin A; d) changing with phenol-red-free culture liquid with astragaloside, and continuously culturing under hypoxic condition; e) subjecting the stem cells and the culture liquid to ultrasonic disruption and centrifuging, collecting supernate, and filtering to remove bacteria; f) detecting total protein concentration of the supernate, and adjusting the total protein concentration of the supernate. By using the preparation method of the stem cell preparation for skin beauty, the key problems of conventional culture methods, such as low in-vitro culture cell quantity and low factor content are solved.

The invention relates to a urease michaelis constant determination method, namely a urease-phenol red method, belonging to the technical field of biochemistry and application enzymology. The determination method provided by the invention is characterized in that a series of urea solutions with different concentrations act as a substrate, and phenol red acts as an indicating agent, urea is decomposed to generate ammonia under the action of urease michaelis, and pH value is increased; the color of a system becomes dark under the action of the phenol red indicating agent, the absorbancy is increased, and is determined by a spectrophotometer or a microplate reader. The enzymatic reaction velocity is in direct proportion to the increase of the absorbancy value, so that the reciprocal 1 / delta A of the absorbancy increase value can replace 1 / V in a linewaver-Burk equation 1 / V=Km / (Vmax[S])+1 / Vmax, and the reciprocal 1 / [S] of the concentration of the substrate urea is plotted; the intercept -1 / Km of a straight line on the X axis can be obtained via the linewaver-Burk equation, and the Km value is resolved.

The invention relates to a method for testing the content of boron trioxide in glass containing zinc and lead, belonging to the technical field of chemical analysis testing. The method comprises the following steps: dissolving a sample by using a conventional glass treatment method (alkali fusion method) to convert boron into borates, dissolving the fused mass in dilute acid, adding carbonates, and separating the borates from other impurity elements; and complexing the amphoteric elements (zinc and lead) by using Ca-EDTA (Ethylene Diamine Tetraacetic Acid); and adding mannitol to quantitively convert boric acid into alcohol boric acid with strong degree of dissociation, and accurately controlling the titration end-point by using a mixed indicator of bromothymol blue and phenol red. The testing method has the advantage of clear discolored points, is easy to control, and can effectively eliminate the interference of amphoteric elements (zinc, lead and the like) in the glass, thereby obviously enhancing the measurement accuracy and repetitiveness. The relative standard deviation of the test is reduced to below 0.5%, and the standard recovery rate is higher than 98%.

A method for the in vivo detection of urease-producing helicobacter in the upper stomach is disclosed. The dense carrier is divided into two separate groups which are combined with separate reagent indicators, one of which also contains urea. The carriers are food soluble products, preferably sugar beads having a diameter of approximately 0.2 to 3.0 mm. The treated carriers and urea are encapsulated in a soluble capsule which is administered to a patient. The density of the carriers cause the capsule to migrate to the gastric mucosa, where the capsule, but not the reagents, is dissolved, placing the reagents and urea in direct contact with the gastric mucosa. The urea reacts with any urease present in the stomach by creating ammonia, which increases the pH in the immediate vicinity of the urea containing carrier and indicator beads. The two reagents react differently, through color change, to the increase in pH, which is viewed through use of an endoscope. A preferred first reagent is bromothymol blue (dibromothymolsulfonphthalein), which changes yellow in the presence of urease, and a preferred second reagent is phenol red (phenolsulfonphthalein), which turns red in the presence of urease.

The invention discloses a preparation method of a swine vaccine specific swine spleen transfer factor (TF). The preparation method comprises the following steps: slaughtering a swine with a positive swine vaccine antibody to harvest the swine spleen; preserving the swine spleen at low temperature; unfreezing the swine spleen; removing fasciae; mincing the swine spleen; homogenizing the pulp; filling the homogenized pulp into a bottle and storing the pulp; repeatedly freezing and thawing the pulp; centrifuging the pulp; carrying out microfiltration; carrying out ultrafiltration; carrying out inactivation; regulating the pH value; regulating the osmotic pressure; removing bacteria; and detecting the quality. The preparation method has the advantages that the pH value of water for injection is regulated to 4-6 in the pulp homogenizing step, thus being beneficial to increasing of the yields of ribose and polypeptide; a box type membrane coating is adopted for tangential flow filtration, so that the dead volumes of system residues are small and linear amplification production is easy to achieve; a 1-3KD box type membrane coating is adopted to carry out tangential flow nanofiltration on a TF crude product, thus preparing the high-concentration TF and improving the using effects; phenol red is used as an acid-base indicator, thus being convenient for clients to observe the pH value of the TF; the TF uses beta-propiolactone for inactivation instead of traditional formaldehyde and an osmotic pressure regulation process is added, thus being beneficial to combined immunization of the TF and vaccines.

The invention discloses an oyster CRISPR / Cas9 gene editing method. The method comprises the following steps: screening a sgRNA target site sequence of a gene to be edited, designing a sgRNA sequence of a target sequence, and performing in vitro transcription to obtain a high-concentration target gene sgRNA; mixing sgRNA with Cas9 protein in equal volume, then reacting at 37 DEG C for 15 minutes, mixing with a phenol red solution in equal volume, introducing the mixture into oyster fertilized eggs by a microinjection method, and detecting oyster embryos or larvae after incubation for 6 hours toobtain corresponding gene mutants. According to the invention, an oyster gene editing technology is successfully established for the first time, and the technology can be used for studying the function of oyster genes and accurately modifying endogenous genes of oysters to provide a technical support for genetic improvement breeding of oysters.

The invention discloses a method for preparing a serum-free soybean protein peptide animal cell medium, and relates to a serum-free or low-serum collective cell medium. The serum-free soybean protein peptide animal cell medium is prepared from calcium chloride, potassium chloride, anhydrous magnesium sulfate, sodium chloride, anhydrous sodium dihydrogen phosphate, L-glutamine, D-glucose, phenol red, D-calcium pantothenate, choline chloride, folic acid, i-inositol, niacinamide, pyridoxal hydrochloride, lactoflavin, thiamine hydrochloride, soybean protein peptide and distilled water. The method of the invention solves the problem that the conventional serum-free medium cannot be directly used for cultivating cells in the poor growth condition, and a large number of the cells can adapt to the conventional serum-free medium only when the serum concentrations thereof are gradually reduced, and the cells are easily affected by external factors, so fatal damages are easily caused. The method of the invention has the advantages that the cells can directly be cultivated in the medium of the invention without gradual domestication and cultivation, so the culture period of the cell is shortened and the interference of other factors is avoided; meanwhile, the virus pollution brought by serums is reduced, and the survival rate of the cells is over 94 percent.

Adipose tissue cryopreservation liquid at a clinical application level and a cryopreservation method. The adipose tissue cryopreservation liquid at the clinical application level comprises 15-50-mmol / L saccharose, 15-50-mmol / L mannitol injection, 1-5-mmol / L glucose injection, 1-3-mol / L adenosine injection, 0.5-5-mmol / L reduced glutathione, 1-4.5-mol / L trehalose and 0.1-1-mmol / L EDTA-2Na; and the components are mixed to form mixed liquid, and the mixed liquid is the adipose tissue cryopreservation liquid at the clinical application level. By means of the adipose tissue cryopreservation liquid,in combination with the cryopreservation method thereof, at the clinical application level, the defect of adverse reaction due to 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), phenol red, DMSO, animal serum, blood platelet extracts and the like which are used in the prior art is effectively overcome.

The invention provides scar removing composition and a dressing comprises a mesenchymal stem cell extract, a fibroblast growth factor and an aloe extract, wherein the mass concentration of the fibroblast growth factor is 10-50 ng / mL, and the mass fraction of the aloe extract is 0.1%-1%; a mesenchymal stem cell culture solution is obtained through sequential culture of P1-P5 generations of mesenchymal stem cells with a mixed culture medium and a phenol-red-free culture medium; the mixed culture medium comprises a DMEM / F12 culture medium and fetal calf serum. The aloe extract is prepared with the following method: epidermis and mesophyll of fresh leaves of aloe vera are separated, then gel juice is squeezed from the mesophyll and sequentially sterilized, concentrated and freeze-dried, and the aloe extract is obtained. The scar removing composition adopts the mesenchymal stem cell culture solution, is low in immunogenicity and harmless to human bodies, is matched with the fibroblast growth factor and the aloe extract and can fade scars and repair skin wound surfaces.

The invention discloses a reagent freeze-dried microsphere for detecting concentrated carbon dioxide binding force and a preparation method of the reagent freeze-dried microsphere. The reagent freeze-dried microsphere comprises a buffer solution, carbonic anhydrase, phenol red, bromothymol blue, a preservative, trehalose, polyethylene glycol, TrionX-100 and the like. The preparation process of the reagent freeze-dried microsphere mainly comprises the following steps: (1) preparing a solution, filtering and degassing; (2) preparing microdroplets by using an accurate quantifying liquid separation system, and dropping the microdroplets into liquid nitrogen to prepare frozen spheres; and (3) transferring the frozen spheres to a freeze dryer to freeze and dry, thereby obtaining the freeze-dried reagent microsphere. A reagent for detecting carbon dioxide binding force disclosed by the invention is a spherical particle freeze-dried reagent, and can be pre-packaged into a detection chip. Compared with an existing liquid detection reagent, the reagent has the advantages that stable preservation and transportation at room temperature can be achieved; and compared with a freeze-dried powder reagent, the reagent has the advantages of being accurate to quantify, convenient to package and the like, and can be suitable for a POCT biochemical analyzer introducing a microfluidic chip technology.

The invention relates to a serum-free culture medium for amplification in vitro of a human immune killer cell, wherein the serum-free culture medium is formed by combining and mixing a basal culture medium and a serum replacement, and the serum-free culture medium comprises the basic components of 2 saccharides, 22 amino acids, 6 lipids, 2 nucleosides, 11 vitamins, 16 salts, 1 hormone, 2 protides, hydroxyethylpiperazine ethane sulfonic acid and phenol red. The serum replacement is combined and added into the basal culture medium to prepare the serum-free culture medium. The components of the serum-free culture medium are definite, and the serum-free culture medium supports amplification in vitro of the immune killer cell, achieves high efficiency and stability, promotes the further study and application of biological therapy, promotes the more smooth progressing of the immune cell therapy, and benefits the nation and the people.

The invention provides a mycoplasma anatis culture medium. The mycoplasma anatis culture medium is composed of a liquid culture medium and a solid culture medium. The liquid culture medium is prepared from deionized water, a 10% thallium acetate solution, PPLO broth, glucose, peptone, tryptone, 1% phenol red, a CMRL-1066 culture medium containing L-glutamine, inactivated horse serum, yeast leachate, penicillin, L-cysteine hydrochloride and nicotinamide adenine dinucleotide. Besides the situation that agar powder is added and phenol red is removed, other components of the solid culture medium are the same as those of the liquid culture medium. The culture medium is used for separating and purifying mycoplasma anatis, wherein a bacterial colony is separated from the solid culture medium, the separated bacterial colony is placed in the liquid culture medium to grow, then filtering, dilution and plate coating of liquid culture substances and purification of picked single colony multiplication are conducted, and the steps are repeated till purified mycoplasma anatis is obtained. The culture medium can promote growth of mycoplasma anatis, and the success rate of separation and purification of mycoplasma anatis is high.

The invention provides sheep embryonic cell culture fluid. The sheep embryonic cell culture fluid comprises the following components: calcium chloride dehydrate, anhydrous magnesium sulfate, potassium chloride, monopotassium phosphate, sodium bicarbonate, sodium chloride, bovine serum albumin, glucose, sodium pyruvate, sodium lactate and phenol red sodium salt. Embryonic fluid at a volume by concentration of 10% is also added to the culture fluid so that the culture fluid has a great cell culture effect. According to the culture fluid, the components collaborate with each other, so that the irritation to an early embryo is reduced, and the embryo can grow well; and in addition, 10% of uterine embryonic fluid of a ewe is added to the culture fluid, so that the fusion rate of a reconstructed embryo can be remarkably increased. Compared with the culture fluid without the embryonic fluid, the culture with the embryonic fluid has the capability of obviously increasing the survival rate and blastocyst rate of in-vitro cultured embryos; and the culture fluid provided by the invention also can improve uterine receptivity, therefore, the lambing percentage is increased obviously.

The invention discloses a serum-free immune cell culture medium. The serum-free immune cell culture medium is prepared mainly from amino acids, vitamins, minerals, D-glucose, 4-hydroxyethyl piperazinyl ethanesulfonic acid, insulin, phenol red, sodium pyruvate and transferrin. The invention discloses another serum-free immune cell culture medium which further comprises cofactors including salidroside, luteolin and resveratrol. The invention discloses another serum-free immune cell culture medium which further comprises activators including an acetyl coenzyme A, a coenzyme Q10, glucose 6-phosphate and cyclic adenosine monophosphate. A part of embodiments of the invention can increase the positive ratio of cell phenotypes.

The invention discloses a rapid testing card for heavy metal mercury and a testing method thereof. The structure of the testing card includes a base board, absorption wafers and a cover film successively from bottom to top. At least one wafer for absorbing color-developing agents and at least one wafer for absorbing urease are respectively fixed on the base board, and the wafers are not overlapped mutually. The use amount ratio of the urease and the color-developing agents absorbed by the wafer per unit area is 3U: (1.6-3.2mL), and the color-developing agents contain components of, by mass, 0.05% to 2.5% of phenol red and 0.8% to 2% of urea. The testing card can test the content of mercury larger than or equal to 0.2 mg / L rapidly, accurately and conveniently; the test card is low in cost, simple and convenient to operate, short in testing time, in no need of professionals, sensitive in reaction and easy to observe and interpret; and the testing card is non-toxic and harmless and cannot cause environment pollution.

The invention relates to genitourinary tract microorganism cultivation and detect method thereof, in particular to a genitourinary tract ureaplasma urealyticum (Uu) and mycoplasma hominis (Mh) culture medium and a detect method thereof. The culture medium comprises a fluid medium and a solid medium which are combined when the medium is used for culturing, separating and differentiating mycoplasma. The formulation comprises essential elements such as tryptic soy broth, yeast extract, urea, arginine, L-cysteine, phenol red, HEPES liquid, calf (fetal calf) serum, manganese sulfate, vancomycin, amphotericin B, trimethoprim, polymyxin, penicillin, and the like, and thereby the nutrition for mycoplasma growth and the antibiotic combination for inhibiting undesired microbe growth are optimized, no millipore filter is needed for sample inoculation and subcultivation, and the mycoplasma grows quickly. The fluid medium can maintain and prolong the logarithmic phase and indicate the growth of the mycoplasma, and the solid medium can used for observing Uu and Mh bacterial colonies, which has large background reflectance, thereby being easy for differentiation and significantly increasing the sensibility and the specificity of genitourinary tract mycoplasma detect.

The invention discloses a chromogenic culture medium for separating and detecting shigella; the culture medium comprises yeast powder, tryptone, soy peptone, sodium chloride, lactose, cane sugar, 2-deoxy-D-ribose, agar powder, beta-glucosaccharase chromogenic substrate, beta-pyranfucosidase chromogenic substrate, beta-galactosidase chromogenic substrate, phenol red, 3# cholate, novobiocin sodium salt, cefsulodin and cefixime; residue is water; the chromogenic culture medium provided by the invention can be used for separating and detecting four species of shigella and has advantages of high specificity, high sensitivity, easy operation, simple result judgment and the like; the chromogenic culture medium is suitable for detecting each sample and has wide application prospect; and detecting effect of the chromogenic culture medium can achieve level of like products in an import R&F company and is superior to inland like products.