38 results about "Phenol red" patented technology

The invention discloses a method for simultaneously determining N-sodium laureth sarcosinate and phenol red residues in an injection based on a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method. According to the method, on the basis of an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer, a 5mM ammonium acetate solution (containing 10% acetonitrile) is taken as a phase A, a 0.2% acetic acid acetonitrile solution is taken as a phase B, a Waters Acquity UPLCBEH C18 column is selected, ESI is taken as a mass spectrometry detection ion source, and the two process residues in the injection are accurately quantified at the same time in a negative ion scanning and multi-reaction monitoring mode. The method is simple in pretreatment, high in detection sensitivity and good in reproducibility, and can be used for corresponding injection process residue identification and quality control.

The present invention provides a visual indicator film for the freshness of fresh-cut fruits, a preparation method, the use and a detection method. The preparation method comprises the following steps: preparing a Co-based MOF; washing and activating the Co-MOF; preparing a CMC-Na-based thin film; and preparing a CMC-Na-based thin film, and thereby obtaining an indicator film for phenol red (PR) or an indicator film for bromothymol blue (BTB). In the present invention, an irregular spherical Co-based metal organic framework (Co-MOF) loaded with PR or BTB is prepared, and the nanocomposites Co-MOF / PR and Co-MOF / BTB are obtained after dyes and the MOF are adsorbed by means of the hydrogen bond interaction. Then, the nanocomposites are embedded into a sodium carboxymethyl cellulose (CMC-Na) base material to prepare CO2-responsive packaging materials, namely, a CMC-Na / PR / Co-MOF and a CMC-Na / BTB / Co-MOF, which are used for monitoring the freshness of fruits in real time and in situ. The Co-MOF-based composite thin film has good water resistance, time-temperature stability and mechanical properties, and also exhibits enhanced ultraviolet-visible light barrier properties, dye migration resistance and thermal stability. The visual indicator film for the freshness of fresh-cut fruits can effectively reflect the concentration of CO2, and is suitable for real-time and in-situ monitoring of the freshness of fruits.

The invention belongs to the technical field of biological medicine, and particularly relates to a mycoplasma synoviae culture medium capable of improving strain titer and a preparation method of the mycoplasma synoviae culture medium. The mycoplasma synoviae culture medium comprises a basic culture medium and auxiliary components, the basic culture medium is prepared from HEPES, milk protein hydrolysate, MEM, beef heart extract powder, coenzyme I, 1% phenol red, magnesium sulfate heptahydrate, L-cysteine hydrochloride, lactose-N-tetrasaccharide, disodium clodronate and water; the auxiliary components comprise porcine serum and penicillin. According to the mycoplasma synoviae culture medium obtained by the preparation method, the passage time of the mycoplasma synoviae is shortened, and the strain titer is improved. The inactivated vaccine prepared from the chicken mycoplasma synoviae cultured by the culture medium is good in safety and immunogenicity, can effectively prevent infection of the chicken mycoplasma synoviae, and has a good application prospect.

The invention discloses a method for detecting the concentration of halogen in liquid chloromethane, which comprises the following steps: S1, preparing an acetic acid-sodium acetate buffer solution, a phenol red solution, a chloramine-T solution and a sodium thiosulfate solution for later use, volatilizing the to-be-detected liquid chloromethane with a fixed volume at normal temperature, and then adding water to dilute the to-be-detected liquid chloromethane to the original volume so as to obtain a test solution A; taking the test solution A with the volume of W1, adding hydrochloric acid to adjust the pH value to 4.4-5, adding water to dilute until the volume is W2, and uniformly shaking to obtain a test solution B; s2, taking N groups of test solutions B, respectively adding an acetic acid-sodium acetate buffer solution with the volume of K1 and a phenol red solution with the volume of K2 into each group of test solutions B, uniformly shaking, adding a chloramine-T solution with the volume of K3, uniformly shaking, adding a sodium thiosulfate solution with the volume of K4, uniformly shaking, adding water to dilute until the volume is W0, uniformly shaking, and standing to obtain N groups of solutions to be detected, and respectively measuring the absorbance of each group of solution to be measured at the wavelength lambda. The method can accurately detect the concentration of the halogen in the liquid chloromethane.

The application belongs to the technical field of biological medicine, and particularly relates to a chicken synovial mycoplasma culture medium capable of improving strain titer and a preparation method thereof. The chicken synovial mycoplasma culture medium comprises a basic culture medium and auxiliary components; the basic culture medium comprises HEPES, milk protein hydrolysate, MEM, beef heart infusion powder, coenzyme I, 1% phenol red, magnesium sulfate heptahydrate, L-cysteine hydrochloride, lacto-N-tetraose, disodium chlormethine and water; and the auxiliary components comprise pig serum and penicillin. The chicken synovial mycoplasma culture medium obtained by the preparation method shortens the passage time of chicken synovial mycoplasma and improves the strain titer. The inactivated vaccine prepared from the chicken synovial mycoplasma cultured by the culture medium has good safety and immunogenicity, can effectively prevent chicken synovial mycoplasma infection, and has a good application prospect.

The present application relates to the technical field of metal content determination, and specifically discloses a long-lasting and stable liquid calcium indicator, comprising an indicator component A and a dilution component B; the indicator component A is obtained by compounding calcein, phenol red, and thymolphthalein; the dilution component B is a glycerol-triethanolamine mixture; the liquid calcium indicator is obtained by adding 1g of calcein, 0.15g of phenol red, and 1g of thymolphthalein to a 100mL glycerol-triethanolamine mixture; the liquid calcium indicator of the present application significantly extends the storage period compared to a solid calcium indicator; the newly prepared liquid calcium indicator can be stored at room temperature for 63 days without deterioration and can accurately indicate the titration endpoint; in addition, the amount of the liquid calcium indicator added can be accurately controlled, specifically by using a pipette, while the solid calcium indicator cannot be accurately controlled.

The invention discloses a pharmacodynamic test method for a cough relieving mixture, which comprises the following steps: constructing four core pharmacodynamic test sub-models containing ammonia water to cause young mouse cough, citric acid to cause young guinea pig cough, rabbit in-vitro trachea cilia mucus movement and young mouse trachea phenol red excretion and a body model of a test result analysis sub-model; each pharmacodynamic test sub-model is provided with a plurality of groups of test arrays and test individuals; pathogen data is imported to generate a simulated disease scene, and a corresponding drug sequence is called to intervene and collect physiological index change data; and multi-dimensional pharmacodynamic parameters are evaluated through the analysis sub-model, and a dose-effect relation curve and an evaluation report are generated. According to the invention, a parallel verification system for antitussive and expectorant tests is formed, the antitussive effective dose, the expectorant effective dose and the effective dose range under the dual action of the medicine are determined through multi-model cross validation, the accuracy degree of judging the effectiveness of the medicine is greatly improved, and a reliable method support is provided for evaluating the efficacy of the cough-stopping mixture.

The invention relates to a helicobacter pylori staining solution for rapid detection, which consists of a staining solution and a diluent, the staining solution comprises the following components in percentage by weight: 0.4-0.6% of phenol red, 30-40% of ethanol and 60-70% of purified water, and the pH range of the staining solution is 6.4-6.6. The diluent comprises the following components in percentage by weight: 10%-14% of urea, 0.01%-0.03% of Triton X-100 and the balance of purified water, and the pH range of the diluent is 6.4-6.6. The volume ratio of the staining solution to the diluent is (1: 4.5)-(1: 5.5). The staining solution is simple and rapid in operation process, and one-step staining is achieved, so that personnel operation is facilitated; the reaction is efficient, color development can be completed within 5-10 minutes, and the method is suitable for rapid detection of scenes and convenient to popularize; the staining solution is high in staining efficiency, easy to observe, low in staining cost and high in market competitiveness; the staining solution is stable in performance, easy to transport and store at normal temperature and beneficial to long-term use of reagents; the staining solution is safe and non-toxic in formula, and is safe to operators and environment.

The present invention provides a bovine sex-sorted sperm sorting solution mediated by a small molecule compound and its application. The sperm sorting solution comprises a sperm sorting protection solution and a sex control functional component. The sperm sorting protection solution contains sodium chloride, potassium chloride, calcium chloride dihydrate, sodium dihydrogen phosphate, glucose, sodium pyruvate, sodium bicarbonate, penicillin-streptomycin, and phenol red. The sex control functional component includes creatine and a 24e drug. The concentration of creatine is 450 μM-550 μM, and the concentration of the 24e drug is 0.27 μM-0.33 μM. The 24e drug is a pyrido [3,2-d]pyrimidine-based TLR7&8 dual agonist. The present invention can meet the large demand for sex-sorted semen in production. Moreover, the sorting process is carried out under the suitable conditions for sperm and does not require complex treatment of sperm. It takes less time for sorting and causes less damage to sperm.

The application discloses a rapid detection method for the cleanliness of a slide for chromosome preparation for visual analysis, and relates to the technical field of chromosome detection, and comprises the following steps: step one, phenol red is added to anhydrous methanol and glacial acetic acid, the mixture is uniformly mixed, sealed and left to stand, after undissolved particles are precipitated, the supernatant clear orange liquid is taken as a detection reagent; step two, the system sampling method is used to screen and detect pathological slides in a whole box, and the same batch of pathological slides without defects and pollution are detected; step three, a plurality of pathological slides that pass the detection are selected and placed obliquely, and the detection reagent is added dropwise; and step four, the cleanliness of the pathological slides is judged. The visual rapid cleanliness detection method has the advantages of simple process, intuitive interpretation, effective satisfaction of the daily quality control demand of the slide for chromosome preparation in a laboratory and popularization value.

The invention relates to the technical field of molecular biology, in particular to an LAMP (Loop-Mediated Isothermal Amplification) double-signal visual detection indicator based on SYTO 9 and application of the LAMP double-signal visual detection indicator. SYTO 9 is adopted as a fluorescent indicator, a phenol red and hydroxynaphthol blue mixture is adopted as a color indicator, and it is found that after SYTO 9 is added, the visual sensitivity is increased from 20 cfu / reaction to 2 cfu / reaction; by means of the method, the low copy number can be detected, the false positive rate is reduced, and the problem that weak positive caused by too low template concentration is difficult to distinguish during photographing retention or visual distinguishing can be perfectly solved. The color indicator provided by the invention can simultaneously use a direct visual method and a real-time fluorescence amplification method, has the characteristics of high resolution, high sensitivity and low false positive rate, realizes the effect of judging whether a sample is positive or negative by naked eyes in combination with double signals of a fluorescence amplification curve, and is high in judgment result accuracy.

A culture medium for testing drug resistance of H. pylori as well as a preparation method and use thereof are characterized in that 10% to 15% of calf serum, 1.2 mg / mL to 2.4 mg / mL of urea, 0.004 mg / mL to 0.016 mg / mL of phenol red, 10 μmol / L to 100 μmol / L of nickel chloride and H. pylori selective additive accounting for 1% of the total volume of the culture medium are added on the basis of a Columbia culture medium, and meanwhile, antibiotics for testing drug resistance are added, and the pH value is adjusted to 7.15 to 7.35. The present invention provides a method for a rapid testing of H. pylori resistance in culture media.

The invention relates to the technical field of microorganism culture and screening, and discloses a culture medium for screening mineralizable microorganisms and a screening method, and the culture medium for screening mineralizable microorganisms contains L-arginine, yeast extract powder, seawater salt, beta-sodium glycerophosphate, sodium silicate, phenol red and ferric sodium ethylene diamine tetraacetate. The culture medium is high in mineralizable microorganism screening pertinence and extremely low in infectious microbe content. According to the culture medium disclosed by the invention, L-arginine is taken as a main nutrient substance, and mineralizable microorganisms often preferentially utilize L-arginine (agmatine is produced through a decarboxylation reaction, the pH is indirectly increased, and the combination of Ca < 2 + > and CO3 < 2-> is promoted); beta-sodium glycerophosphate is added into the culture medium, so that'carbonate mineralizing bacteria '(utilizing Ca < 2 + > + CO3 < 2->) and'phosphate mineralizing bacteria' (utilizing Ca < 2 + > + phosphate radical) can be screened at the same time; the added sodium silicate is only utilized by silicate mineralizing bacteria. The types of mineralization bacteria screened by the culture medium cover three mineralization types of carbonate, phosphate and silicate.

The invention relates to the field of microbiological detection, in particular to an LAMP (loop-mediated isothermal amplification) primer group for visual rapid detection of Brucella as well as a kit and application of the LAMP primer group. The LAMP primer group for visual rapid detection is designed aiming at a bcsp31 gene sequence and comprises a forward outer primer (F3), a reverse outer primer (B3), a forward inner primer (FIP-T), a reverse inner primer (BIP-T), a forward loop primer (LF) and a reverse loop primer (LB). When the kit is used, DNA of a sample to be detected is extracted, the primer group is added, a fluorescent dye or methylphenol red is added, an amplification reaction is carried out, and whether the sample to be detected contains brucella or not is judged according to a color development result. The invention provides an LAMP (loop-mediated isothermal amplification) primer group, a kit containing the primer group and application of the LAMP primer group, wherein the LAMP primer group takes a bcsp31 gene as a reference sequence, is shorter in response time, high in sensitivity, good in compatibility and suitable for visual detection of Brucella in daily frequent public health.

The invention discloses a visual paper-based cancer miRNA marker detection kit, the kit comprises an integrally formed foldable T-shaped filter paper, and a DNA probe TEprobe, urease and a color developing agent dropwise added on the filter paper, and the principle of the kit is as follows: the TEprobe is combined with a cancer nucleic acid marker (miRNA) to release Ag < + > to inhibit the activity of the urease; the composite signal response of the pH response probe phenol red and fluorescein is triggered, and the detection sensitivity is as low as 10-12 M. The kit provided by the invention can significantly shorten the detection time and reduce the sample demand, and realizes zero false positive rate and 97.10% specificity by combining with machine learning algorithm classification during result analysis. The performance is successfully verified in real samples of prostatic cancer, liver cancer and colorectal cancer, and a cheap and convenient means is expected to be provided for family self-inspection and cancer screening and diagnosis in resource-deficient areas.

The invention relates to the technical field of biological medicines, and discloses application of urolithin A in a culture solution for reducing fragmentation of aged oocytes. The culture solution comprises NaCl, KCl, KH2PO4, MgSO4. 7H2O, glucose, NaHCO3, sodium pyruvate, sodium lactate, CaCl2. H2O, gentamicin, phenol red and urolithin A. The final concentration of the urolithin A is 25 mu M-50 mu M. The invention provides the culture solution for improving the quality of the oocytes, a feasible scheme is provided for obtaining more and better oocytes, and help is provided for improving the assisted reproduction efficiency clinically.

This invention relates to the field of traditional Chinese medicine research technology, specifically a method for discovering and identifying quality markers for the antitussive and expectorant effects of Ke Ning granules. This application evaluates the antitussive and expectorant effects of Ke Ning granules using concentrated ammonia-induced cough and phenol red excretion assays. Plasma pharmacochemistry studies of Ke Ning granules were conducted using UHPLC-Q-Exactive Plus Orbitrap HRMS technology, identifying 26 prototype blood-entering components. Through network pharmacological analysis of these blood-entering components, 11 potential active ingredients for antitussive and expectorant effects of Ke Ning granules were screened, involving 40 common targets including IL6, TLR4, and STAT3, and mainly participating in signaling pathways such as PI3K-Akt, HIF-1, and EGFR. Seven prototype blood-entering components were quantitatively analyzed using pharmacokinetic methods. Azelaic acid, caffeic acid, and vanillin were selected as quality markers for the antitussive and expectorant effects of Ke Ning granules based on efficacy, transmissibility, and measurability. The results of this study are of great significance for further clarifying the pharmacodynamic material basis of Ke Ning granules, optimizing quality standards, and promoting its clinical application.

This application discloses a serum-free cryopreservation solution for cells and its applications. The serum-free cryopreservation solution comprises: 1-2% recombinant human albumin, 5-10 μM (-)-Blebbistatin, 50-100 μM antioxidant, 5-10% permeable cryoprotectant, and a basic culture medium free of phenol red indicator. This serum-free cryopreservation solution is suitable for the cryopreservation of various cell types, particularly muscle cells, especially human induced pluripotent stem cell (iPSC) differentiated cardiomyocytes. It can significantly improve the survival rate, structural integrity, and effective preservation of the physiological functions of cardiomyocytes after resuscitation. It is applicable to basic scientific research, disease modeling, drug screening, and clinical treatment related to cardiomyocytes.

The application discloses a kind of based on olfactory visualization chip beef freshness rapid detection method, comprising the following steps: selecting tetramethoxy phenyl porphyrin iron (III) chloride and phenol red as gas-sensitive material, and preparation is obtained with two sensors olfactory visualization chip;Different storage time under the representative beef sample is set, freshness index data is combined with the response characteristic matrix obtained corresponding storage time, to establish the back propagation neural network quantitative model of beef freshness.The response characteristic matrix of the olfactory visualization chip of the beef sample to be measured is input into the quantitative model established in beef, to obtain the predicted value of TVB-N content and the predicted value of TVC content of the beef sample to be measured.The application can realize rapid, simple, accurate quantitative detection of beef freshness;To solve the shortcomings of the prior art detection process, high detection cost, easy to be disturbed by environment and unable to detect visualized etc.

The invention discloses a dressing material for naked eye visual wound infection monitoring and a preparation method thereof, and the preparation method comprises the following steps: adding thionyl chloride into a mixed system of carboxymethyl cellulose fiber and a solvent to obtain acylating chlorinated carboxymethyl cellulose fiber so as to activate carboxyl; and mixing the carboxymethyl cellulose fibers with pH-responsive color-changing dyes such as bromothymol blue or phenol red, and heating to react, so as to obtain the carboxymethyl cellulose fibers covalently grafted with the pH-responsive color-changing dyes. Carboxyl on carboxymethyl cellulose is subjected to acylating chlorination, the reaction activity of the site is improved, the site can chemically react with phenolic hydroxyl on the pH-responsive color-changing dye, the carboxymethyl cellulose fiber covalently grafted with the pH-responsive color-changing dye is obtained, the risk of dye exudation is greatly reduced, and excellent safety is achieved. The dressing material obtained by the invention has an excellent pH-responsive discoloration function, can generate pH-responsive discoloration in a key pH range (pH 6-8) of wound infection indication, achieves the purpose of wound infection indication, and has a wide actual clinical application prospect.

This invention belongs to the field of molecular biology technology, specifically relating to a liquid- and lyophilized dual-purpose LAMP reaction colorimetric premix, lyophilized formulation, and preparation method. The premix comprises: BST DNA polymerase, magnesium sulfate, potassium chloride, phenol red, BSA, mannitol, trehalose, and a mixture of dNTPs. This premix is ​​a completely exogenous buffer-free LAMP red-yellow colorimetric premix, achieving a seamless, abrupt red-yellow colorimetric transition, completely eliminating human interpretation errors caused by intermediate colors, and achieving 100% accuracy for non-professional interpretation. By completely eliminating buffer components, the cause of pH drift during lyophilization is eliminated at its source. Combined with a ternary synergistic protection system, the pH fluctuation before and after lyophilization is <0.1, the phenol red background remains stable over a long period, and the colorimetric window does not decay. The same formulation is suitable for both direct use in fresh liquid form and integrated lyophilization after mixing with primers, eliminating the need for separate formulation development for different scenarios.

The utility model relates to the technical field of sleeping bags, in particular to a baby sleeping bag with a urine display function, which comprises a sleeping bag main body, a wind blocking strip and a strip-shaped mute cloth loop surface magic tape are arranged on the front side of the sleeping bag main body and close to a wearing opening, and a strip-shaped mute cloth hook surface magic tape bonded with the strip-shaped mute cloth loop surface magic tape is arranged on the rear side of the wind blocking strip. Two pieces of first transparent cloth are sewn to the bottom of the front side of the sleeping bag body, second transparent cloth is sewn to the bottom of the rear side of the sleeping bag body, and a detachable urine display part is arranged at the bottom of the rear side of the inner wall of the sleeping bag body. According to the baby sleeping bag with the urine display function, when a baby diaper leaks urine, the urine drops on the non-woven fabric and permeates into the indicating layer from the non-woven fabric, and the pH change of the urine causes the change of phenol red color, so that a visual signal is provided to remind parents that the urine is in contact with the urine display part, and the baby sleeping bag has the urine display function.

This invention provides a composition with antitussive and expectorant effects and its preparation method. The composition comprises raw materials such as Selaginella uncinata, Smilax glabra, Ficus microcarpa, Erythrina variegata, Basil, Cinnamomum cassia, Bambusa textilis, Rehmannia glutinosa, Prunus armeniaca, Pinellia ternata, Fritillaria thunbergii, and Magnolia officinalis. This invention uses a topical patch for administration through acupoint application, which can significantly prolong the cough latency period, reduce the frequency of coughs, and increase the amount of phenol red secreted from the trachea, exhibiting significant antitussive and expectorant effects. It addresses the root cause by tonifying Qi, resolving phlegm, relieving cough, and regulating Qi, and is suitable for coughs with excessive phlegm caused by spleen deficiency and internal dampness, and heat toxicity obstructing the lungs in the Lingnan region.

The invention provides a salmonella rapid ratio colorimetric detection method based on a hybridization chain reaction, and relates to the technical field of biosensing and nucleic acid detection, and the salmonella is adsorbed and enriched by Fe3O4 (at) PDA (at) PEI magnetic nanoparticles through electrostatic interaction. A salmonella specific hairpin aptamer is used for identifying and triggering a hybridization chain reaction (HCR), and a DNA sequence connected with urease is introduced as a signal detection unit. Phenol red is used as an indicator, and the absorbance and the solution color change in a ratio mode. The kit is rapid, sensitive, specific and easy to use, the sample treatment process is reduced, and the sample detection efficiency is improved; the method can be directly operated at room temperature without complex operation environment, and is convenient to use.

Provided is a transfection reagent, comprising a solvent, organic substances, amino acids, vitamins and inorganic salts, wherein the organic substances comprise D-glucose, sodium pyruvate, i-inositol, phenol red and choline chloride; the amino acids comprise glycine, L-glutamine, L-valine, L-isoleucine, L-leucine, L-threonine, L-lysine hydrochloride, L-phenylalanine, L-serine, L-tyrosine disodium salt, L-arginine hydrochloride, L-methionine, L-cystine dihydrochloride, L-histidine hydrochloride and L-tryptophan; the vitamins comprise nicotinamide, pyridoxine hydrochloride, thiamine hydrochloride, folic acid, D-calcium pantothenate and riboflavin; and the inorganic salts comprise sodium chloride, sodium bicarbonate, potassium chloride, calcium chloride, sodium dihydrogen phosphate, magnesium sulfate, hydrochloric acid and ferric nitrate. The transfection reagent is suitable for a wide range of applications, achieves the rapid onset of transfection, and has a good transfection effect.

This invention belongs to the field of LAMP detection reagent technology, specifically relating to a fluorescence / colorimetric dual-mode LAMP detection reagent, its preparation method, and its application. The fluorescence / colorimetric dual-mode LAMP detection reagent of this invention comprises: BST DNA polymerase, magnesium sulfate, potassium chloride, phenol red, BSA, mannitol, trehalose, a mixture of dNTPs, a DNA-binding fluorescent dye, and ammonium sulfate. This fluorescence / colorimetric dual-mode LAMP detection reagent of the present invention simultaneously contains a phenol red colorimetric system and a SYBR Green I fluorescence system, allowing the same reaction tube to be read using a fluorescence instrument or observed visually. It eliminates the need to pre-select reagent types based on the detection scenario, providing the flexibility of "one tube, two modes" to adapt to more diverse detection needs. By introducing ammonium sulfate as a reaction enhancer, the different requirements of the two reading modes for the ionic environment are balanced, achieving "dual excellence" in both fluorescence signal and colorimetric effect.

The invention discloses a preparation method and application of a multifunctional chemical culture medium for mycoplasma culture and detection. The culture medium consists of 80-95 parts of a basic culture medium, 2-8 parts of a specific growth promoting factor, 0.5-3 parts of a selective inhibitor, 1-5 parts of a multifunctional indicator and 1-4 parts of trehalose. Wherein the specific growth promoting factor is a compound of arginine and glutamine, the selective inhibitor is a compound of amphotericin B and thallium acetate, and the multifunctional indicator is a compound of phenol red and triphenyltetrazole chloride. The preparation method comprises the following steps: dissolving the basic culture medium, adding the components, stirring and mixing, adjusting the pH value to 7.6-7.8, subpackaging, inflating and storing. According to the culture medium, by optimizing the synergistic effect of the components, the proliferation rate (up to 90% or above) and the detection sensitivity (the lowest detection limit is 10 CCU / mL) of the mycoplasma are remarkably improved, the positive detection time is shortened to 42-48 h, efficient culture and rapid and visual interpretation of the mycoplasma are achieved, and a reliable solution is provided for clinical diagnosis.

The invention discloses an all-fiber piezoelectric sensing film with a Janus structure as well as a preparation method and application of the all-fiber piezoelectric sensing film, and belongs to the technical field of sensors. The full-nanofiber Janus membrane with one-way liquid transmission and multi-mode sensing functions is prepared through a two-step electrostatic spinning method. The preparation method comprises the following steps: firstly, performing electrostatic spinning on a polyvinylidene fluoride-hexafluoropropylene spinning solution doped with MXene nanosheets to form a piezoelectric functional layer PM membrane; and then, by taking the PM film as a substrate, carrying out secondary electrostatic spinning on a PAN spinning solution loaded with a phenol red indicator to form a hydrophilic sensing layer PR film, and finally forming the Janus structure composite film. Efficient self-powered physical signal sensing is achieved through the high piezoelectric coefficient of the membrane, the membrane is endowed with asymmetric wettability through the Janus structure, directional management of sweat can be achieved, visual colorimetric detection of the pH value of sweat is achieved in combination with phenol red, and the membrane has wide application prospects in the fields of dynamic health monitoring, exercise rehabilitation, personalized medical treatment and the like.

The invention provides an antibacterial hemostatic gel patch and a preparation method thereof, and the method comprises the following steps: taking 3% w / v of cross-linked sodium carboxymethyl cellulose as a hydrogel matrix carrier, uniformly dispersing 2% w / v of chitosan and 0.1% w / v of polyhexamethylene biguanide in a hydrogel matrix, injecting into a mold, and freeze-drying to form inner-layer gel with a slightly convex arc-shaped surface; preparing a mixed solution containing 50% w / v of medical glycerin and 0.5% w / v of sodium hyaluronate, and carrying out film casting on the mixed solution to form a middle buffer layer; the method comprises the following steps: by taking a medical polyurethane film as a base material, coating a phenol red derivative on the inner side of the polyurethane film as a color indicator to form an outer-layer polyurethane film; the middle buffer layer and the inner-layer gel are attached to form hydrogen bond combination through hydroxyl and amino, the middle buffer layer and the outer-layer polyurethane film are bonded through medical acrylate adhesive, and the gel patch is prepared. The gel patch can realize multiple functions such as rapid hemostasis, continuous antibiosis, moisturizing and repairing, and intelligent errhysis monitoring, and is suitable for nursing of puncture points after liver cancer radiofrequency ablation.

The present invention discloses the use of the efcab2 gene in constructing an animal model of hydrocephalus and a method for constructing the model. This invention belongs to the technical field of animal model preparation in biological and basic medical research. The invention provides the use of the efcab2 gene in constructing an animal model of hydrocephalus, and the nucleotide sequence of the efcab2 gene is shown as SEQ ID NO. 1. A method for constructing a zebrafish animal model of hydrocephalus is also provided, comprising: synthesizing guide RNA (gRNA) and Cas9 mRNA based on the efcab2 gene, and mixing them to obtain a mixture; co-injecting the mixture with phenol red reagent into zebrafish embryos at the I-cell stage, culturing them until the third day after fertilization to obtain zebrafish embryos; measuring the expression level of the efcab2 gene mRNA, and observing the fourth ventricle, spine, and pericardium. Using CRISPR gene knockout technology, an animal model of hydrocephalus caused by efcab2 gene deficiency is established to elucidate the pathogenesis and potential therapeutic targets of efcab2 gene-deficient hydrocephalus.