14 results about "Hypoxanthine" patented technology

The present application relates to the field of food processing technology, and provides a method and a device for purine reduction, and an equipment and an electronic equipment and a storage medium. The method for purine reduction includes the following steps: first cooling step: performing first cooling treatment on a food material to be treated at a first preset cooling rate to reduce a temperature of the food material to be treated to a temperature below a freezing point. By performing first cooling treatment on a food material to be treated at a first preset cooling rate to reduce a temperature of the food material to be treated to a temperature below a freezing point, the activity of ATP-degrading enzyme in the food material to be treated can be rapidly inhibited, which can quickly and effectively inhibit the generation of hypoxanthine, thereby reducing the generation of hypoxanthine, reducing the risk of hyperuricemia and gout, and having little effect on the taste and quality of the food material.

This invention discloses *Pediococcus lactis* TC-4, which simultaneously degrades nucleosides, purines, and uric acid, and its applications. *Pediococcus lactis* ( Pediococcus acidilactici This invention relates to *Pediococcus lactis* TC-4, with accession number GDMCC No: 67936. *Pediococcus lactis* TC-4 of this invention can efficiently degrade three nucleosides (guanosine, adenosine, and inosine), three purines (guanine, xanthine, and hypoxanthine), and uric acid in vitro. *Pediococcus lactis* TC-4 of this invention can effectively reduce serum uric acid levels in broiler chickens, alleviating and treating hyperuricemia. Compared with traditional chemical drugs for treating hyperuricemia and / or gout, this invention has no toxic side effects on the ecological environment, no residue risk, and is green and safe. This invention has been confirmed at multiple levels, including genetic and animal studies, to have no potential harm to humans, demonstrating its advantages of safety and high efficiency.

To provide a screening method for a human Na+ dependent hypoxanthine transporter inhibitor to be a candidate substance for an agent for treating or preventing chronic kidney disease, particularly xanthinuria.SOLUTION: The screening method includes the step for bringing a cell expressing human SLC23A3 (solute carrier family 23 member 3) gene product into contact with a test substance in the presence of hypoxanthine and sodium ions.SELECTED DRAWING: None

The application discloses a bird's nest peptide for inhibiting xanthine oxidase and application thereof, and belongs to the technical field of functional polypeptides. The amino acid sequence of the bird's nest peptide for inhibiting xanthine oxidase is WGLL, can chelate an active center of xanthine oxidase (XOD), and is helpful for inhibiting the activity of XOD in catalyzing conversion of xanthine or hypoxanthine. The application further provides a bird's nest peptide powder containing the bird's nest peptide for inhibiting xanthine oxidase. The bird's nest peptide powder can significantly reduce the rate of conversion of xanthine or hypoxanthine into uric acid catalyzed by XOD, has good uric acid reducing effect, can be applied to development of uric acid reducing products or various uric acid reducing products, and has high market value.

A dual-modal quantitative detection method for hypoxanthine to determine meat freshness includes the following steps: Step 1: Measuring the RGB values ​​of the color signal after the sample reaction is complete; Step 2: Measuring the temperature T of the reaction solution; Step 3: Constructing a linear equation between hypoxanthine concentration and color signal B / G or temperature T; Obtaining the color signal B / G based on a series of standard tests at different concentrations, and constructing the linear equation B / G = XC. Hx +y, where C Hx The concentration of the standard is given; based on the temperature T obtained from the testing of a series of standard concentrations, a linear equation is constructed: T = XC. Hx +y, where C Hx For the standard concentration; Step 4: Take the sample to be tested and repeat steps 1 and 2 to obtain the color signal B / G. n and temperature T n B / G n and T n Substituting the values ​​into the corresponding linear equation, the concentration of hypoxanthine in the sample is obtained. This invention employs a colorimetric-photothermal dual-modal detection method, which improves the reliability of the detection.

This invention provides a kit for the simultaneous detection of nine flavor nucleotides in livestock and poultry products. The flavor nucleotides are cytidine monophosphate, uridine monophosphate, guanylic acid, inosine monophosphate, adenosine monophosphate, hypoxanthine, inosine, adenosine diphosphate, and adenosine triphosphate. The kit includes: a high-concentration extraction reagent, a standard curve solution, a high-concentration phosphate buffer solution, pH adjustment solution A, and pH adjustment solution B. The high-concentration extraction reagent is perchloric acid; the standard curve solution is a mixed solution of the nine flavor nucleotides; the high-concentration phosphate buffer solution is a solution of potassium dihydrogen phosphate and dipotassium hydrogen phosphate; pH adjustment solution A is sodium hydroxide; and pH adjustment solution B is phosphate. This invention also provides a method for the simultaneous detection of the nine flavor nucleotides using the above kit. This invention enables the simultaneous determination of the content of nine flavor nucleotides in livestock and poultry products. The method is simple, rapid, accurate, and sensitive, and can be used for batch determination, showing good prospects for practical application.

The application provides a paper-based enzyme biosensor for hypoxanthine detection and a preparation method thereof, and comprises paper-based chip manufacturing, enzyme solution, silicon precursor solution and mineralization system solution required by a synthesis xanthine oxidase silicon enzyme method, color developing system configuration, paper-based chip modification and packaging. Through the technical scheme of the application, xanthine oxidase (XOD) is combined with color developing agent nitro blue tetrazolium chloride (NBT), one-step color development is realized, the operation process is significantly simplified, and the stability of the color developing system is improved. The enzyme biosensor constructed based on the biomimetic mineralization immobilization system can maintain high enzyme activity after being stored at room temperature for 21 days, exhibits excellent room temperature storage stability, is more convenient for practical application and storage and transportation, and greatly reduces the use amount of enzymes, thereby saving costs while ensuring high sensitivity and reliable quantitative detection performance.

The invention relates to the technical field of biological fermentation, in particular to lactobacillus rhamnosus and application thereof. The preparation method of the fermented radix puerariae extract comprises the following steps: adding water into the radix puerariae extract, carrying out high-temperature sterilization, adding lactobacillus rhamnosus LK-A28 according to an inoculum size of 3%, and carrying out inoculated fermentation, so as to enhance the inhibition effect of the radix puerariae extract on xanthine oxidase. The fermented kudzuvine root extract can regulate hyperuricemia caused by purine metabolism disorder, oxidation of hypoxanthine and xanthine into uric acid is reduced by inhibiting xanthine oxidase XOD, the fermentation condition of the fermented kudzuvine root extract is simple, the xanthine oxidase activity inhibiting capacity is remarkably improved after fermentation, industrial production is easy, and the fermented kudzuvine root extract is suitable for industrial production. Good development and application prospects are realized in treatment of hyperuricemia.

The present application relates to a method for targeting editing RNA based on LEAPER technology, which comprises safely and effectively performing in vivo editing of adenosine to hypoxanthine base on RNA by using LEAPER technology, accurately repairing pathogenic mutation sites, and achieving the purpose of treating all diseases caused by G>A mutations, such as MPS IH.

The application discloses a wheat Tilletia indica-specific biomarker combination, a screening method and application thereof, and the biomarker combination comprises 20-hydroxyarachidonic acid, 1-octadecyl lysophosphatidic acid, linolenic acid, 13-hydroxydocosahexaenoic acid, 4-methoxy estrone, hypoxanthine, 3-hydroxymyristic acid, beta-ecdysterone, 17-phenyltrinorprostaglandin F2 alpha cyclopropylmethylamide, 11,12-dihydroxyeicosatrienoic acid and oxidized octadecadienoic acid. When the biomarker combination is used for identifying Tilletia indica, the time-consuming long and the accuracy easily interfered by the spore development stage limitations of traditional morphological identification are overcome; in view of the molecular level identification difficulty caused by the high genome homology of Tilletia indica and Tilletia controversa, the application digs the feature difference at the level of metabolomics, and constructs a specific chemical fingerprint, so that the technical bottleneck that the close relatives are difficult to accurately distinguish is effectively solved.

The application belongs to the technical field of molecular biology, and particularly relates to a detection method and a detection kit for a poly-A tail of RNA. The first aspect provides a detection method for a poly-A tail of RNA, comprising the following steps: incorporating first dNTPs into RNA by reverse transcriptase to synthesize a complementary DNA chain; amplifying and sequencing the complementary DNA chain by using DNA polymerase and taking second dNTPs as a substrate; and analyzing the length of the poly-A tail according to the sequencing result. The application develops a method for replacing raw materials based on deoxyinosine triphosphate (dITP) of hypoxanthine, in which, after template conversion of RNA to form cDNA, the pairing mechanism of dITP and other bases is used to replace the poly-A chain with a random chain, so that a relatively complex sequence can be obtained, the stability of the sequencing signal is improved, and the length of the poly-A tail can be more accurately obtained based on the complex sequence.

Methods of diagnosing Crohn's disease and ulcerative colitis in subjects is provided based on the determination of metabolites in urine samples, such as serine, hypoxanthine, kynurenine, threonine, indoxylsulfate, phenylacetylglutamine, 5-hydroxy-6-indolyl-O-sulfate, 5-(δ-carboxybutyl) homocysteine, sialic acid, guanidinosuccinic acid (GSA), glutamyl(iso) leucine, trigonelline, cresol sulfate, an anion having m / z: RMT: polarity of 345.1553:0.770: n, aminohippurate: GSA ratiometric biomarker, unknown anion (160.0615:0.830: n): tyrosine ratiometric biomarker and [GSA×Tyr]: [AHA×(an anion having m / z: RMT: polarity of 160.0615:0.830: n)] ratiometric biomarker.

The application discloses application of a 5'-monophosphate nucleotide composition in preparation of anti-aging and longevity drugs and / or functional foods, and belongs to the technical field of medicines.The composition is composed of 5'-monophosphate adenosine, 5'-monophosphate cytidine, 5'-guanylic acid disodium, 5'-uridylic acid disodium and hypoxanthine nucleotide, and the mass ratio of the acid types of CMP, AMP, UMP, GMP and IMP is respectively as follows: CMP: 15-78%, AMP: 6-44%, UMP: 7-40%, GMP: 7-51%, and IMP is 0 or greater than 0 and not higher than 2.5%. The composition can synergistically enhance various mechanisms such as significantly reducing an insulin resistance index, enhancing body anti-coagulation function, improving blood circulation, regulating metabolism of essential minerals such as lithium and copper and regulating intestinal flora ecology, delays aging and prolongs healthy life span. Animal experiment data show that, compared with a control group, the composition prolongs the median survival time of mice by 9.21-12.6%, and the equivalent human life span is 8.76-12.01 years. Human experiment data show that, compared with the control group, the composition intervention reduces the median age of DNA methylation by 3.08 years, and provides a new idea for developing anti-aging and longevity drugs and / or functional foods.

This invention discloses two strains of *Lactobacillus reuteri* that are highly efficient at degrading nucleosides and their applications. *Lactobacillus reuteri* ( Limosilactobacillus reuteri The *Lactobacillus reuteri* strains W6-12 and W6-5, with accession numbers GDMCC No. 67938 and GDMCC No. 67937, respectively, exhibit efficient in vitro degradation of adenosine, guanosine, and inosine. In vivo, they significantly reduce serum uric acid levels in HUA broiler chickens induced by high calcium and high protein intake. Their fermented milk significantly reduces serum uric acid levels in SD rats with hyperuricemia induced by potassium hypoxanthine and hypoxanthine. This invention has been confirmed at multiple levels, including genetic and animal studies, to pose no potential harm to humans, demonstrating its safety and high efficiency.