105 results about "Hypoxanthine" patented technology

The invention provides a perovskite solar cell, a preparation method thereof and a photovoltaic module. The perovskite solar cell comprises a first electrode, a hole transport layer, a perovskite layer, an electron transport layer and a second electrode which are stacked in sequence. The material of the hole transport layer comprises a biomolecular material which is fixed on a self-assembly monomolecular layer material through an intermolecular force, and the biomolecular material comprises one or more of guanine, adenine, thymine, uracil, cytosine, hydroxymethylcytosine, xanthine, hypoxanthine, 6-mercaptopurine and corrin. According to the perovskite cell provided by the invention, the biomolecular material with a plurality of nitrogen heterocyclic structures and amino groups is added to generate multipoint hydrogen-bond interaction and pi-pi accumulation with the tail end of the SAM, so that the orderliness of molecular arrangement is improved, and the dipole direction and strength of the whole interface are regulated and controlled; and a coordination bond or hydrogen bond network is formed by the coordination compound and uncoordinated cations or anions in perovskite, so that interface defects are effectively passivated, and the thermal stability and the humid and hot life of a device are enhanced.

The invention belongs to the technical field of gestational diabetes diagnosis marker screening, and particularly relates to a biomarker for predicting or diagnosing gestational diabetes and application of the biomarker. The invention discloses a biomarker for predicting or diagnosing gestational diabetes mellitus, the biomarker is selected from one or more of hypoxanthine, cortisol, dihydrotachysterol and cycloleucine, and more preferably, the biomarker comprises hypoxanthine, cortisol, dihydrotachysterol, (3R, 4R)-D-ketolactone, creatine, cycloleucine and L-hydroorotic acid. The seven biomarkers disclosed by the invention can predict or diagnose gestational diabetes mellitus independently or jointly through plasma detection at the early pregnancy stage, effectively improve the early diagnosis rate of gestational diabetes mellitus and improve disease prognosis, and have clinical use and popularization values.

It is unequivocal that to facilitate targeted antimicrobial prescribing practices and to help reduce the increasing global burden of antibiotic resistance, there is an urgent need for the development of novel and truly rapid (less than or equal to 1 hour) AST platforms. A 785 nm SERS based methodology is described that results in accurate MIC determinations for all tested strain / antibiotic pairs following a 30 minute drug incubation period. The time for an SERS AST result is -1 hour. The SERS-AST method works for both Gram positive and Gram negative species and for all primary mechanisms of antibiotic activity. Agreement with 24 hour growth determined MICs is obtained for both bactericidal and bacteriostatic antibiotics although different patterns of dose dependent SERS intensities characterize these two types of antibiotics. Bacterial SERS spectra due to secreted purine nucleotide degradation products (principally adenine, guanine, xanthine and hypoxanthine).

The invention discloses a preeclampsia mouse model. The preeclampsia mouse model is constructed by the following method: (1) combining a female mouse with a male mouse; (2) in the middle stage of pregnancy, intragastric administration of hypoxanthine and oteracil potassium salt is carried out on the pregnant mouse for a period of time; the invention also discloses a construction method and application of the gene. The invention provides a novel preeclampsia modeling mode, hypoxanthine and oteracil potassium salt are combined for use, and the administration dosage and the administration mode of hypoxanthine and oteracil potassium salt are controlled, so that the serum uric acid level of a model mouse is stably increased, preeclampsia change occurs in the model mouse, and the model mouse can be used for treating preeclampsia. The stability, specificity and pathophysiology characteristics of the model are ensured to be similar to those of human preeclampsia, so that the uric acid-induced preeclampsia mouse model is successfully constructed. The uric acid induced preeclampsia mouse model provides a valuable tool for researching the pathogenesis of preeclampsia, evaluating a new treatment method and understanding the effect of serum hyperuricemia in preeclampsia.

The invention belongs to the field of traditional Chinese medicine prescription extraction process optimization, and particularly relates to a method for optimizing a classic famous prescription Xiaoxiaoxiao decoction extraction process based on orthogonal combination benchmark correlation degree and an AHP-entropy weight method. According to the invention, quality evaluation is carried out on the extraction process by taking the quality of a reference sample as a reference standard, orthogonal design is selected, and a relatively comprehensive quality control system taking the contents of vanillic acid, hypoxanthine, jateorhizine, coptisine, palmatine and berberine as well as fingerprint similarity and dry extract rate as comprehensive evaluation indexes is established; the limitation of the traditional single index on the quality control of the extraction process is avoided. A combined weighting method combining AHP and an entropy weight method is adopted, the defects caused by single weighting are overcome, and a reference is provided for modern preparation development of the classic famous prescription Xiaoxiaoxi decoction.

The present invention provides a method for producing ribose-1-phosphate that is simple and yields improved efficiency. [Solution] A method for producing ribose-1-phosphate, comprising a contact step to produce ribose-1-phosphate and hypoxanthine by contacting inosine, a phosphate donor, an enzyme having purine nucleoside phosphorylase activity, and a medium in a reaction solution containing inosine, a phosphate donor, an enzyme having purine nucleoside phosphorylase activity, and a medium, wherein in the contact step, the hypoxanthine is present in an amount exceeding the saturation concentration relative to the medium.

The invention belongs to the technical field of liquid phase detection, and particularly relates to a quality control and detection method for lumbricus soaked with liquorice. According to the method, methanol water is used as a mobile phase, the content of hypoxanthine and inosine in the lumbricus glycyrrhiza is detected by high performance liquid chromatography, and the method has important significance on quality control of a lumbricus glycyrrhiza product. The detection method disclosed by the invention is good in system applicability, precision, reproducibility, recovery rate and stability, and meets the requirements of Chinese Pharmacopoeia, Chinese Pharmacopoeia, appendix of Chinese Pharmacopoeia, Chinese appendix of Chinese Pharmacopoeia, Chinese Pharmacopoeia, Chinese

The invention belongs to the field of in-vitro diagnostic reagents, and particularly relates to application of a reagent for detecting biomarkers in serum in preparation of a cerebral arterial thrombosis diagnostic kit. The invention determines that the concentrations of lactic acid, isoleucine, fumaric acid, ketoglutaric acid, hypoxanthine, tyrosine, histidine and inosine in serum of a cerebral arterial thrombosis (IS) patient are significantly related to major atherosclerosis (LAA) and minor artery occlusion (SAO), so that the concentrations of the biomarkers can be used as a basis for diagnosing IS and distinguishing LAA and SAO.

The invention relates to the technical field of bioengineering, in particular to an AimA mutant as well as a construction method and application thereof. It is found for the first time that the activity of glutamic acid / serine transporter is weakened, and the nucleoside production capacity of microorganisms can be improved. Based on this, the present invention provides a modified microorganism in which the glutamic acid / serine transporter activity is reduced or lost as compared to an unmodified microorganism, and the nucleoside production capacity of the modified microorganism is enhanced as compared to the unmodified microorganism, and more specifically, the modified microorganism has a reduced or lost glutamic acid / serine transporter activity as compared to the unmodified microorganism. The modified bacillus subtilis or bacillus amyloliquefaciens provided by the invention can generate guanine nucleoside or hypoxanthine nucleoside with high efficiency and high speed.

Methods of treating, reducing the risk of, preventing, or alleviating a symptom of inflammation or inflammatory gastrointestinal disease by administration of a modified bacterium providing purines, in particular hypoxanthine, to the mucosa of the intestine, and compositions containing modified bacteria useful in such methods.

A probiotic composition for inhibiting the onset and progression of colorectal cancer, comprises one or more of a culture supernatant of Faecalibacterium prausnitzii (Fp) cells, live Fp cells, or non-viable Fp cells，wherein the probiotic composition comprises at least one metabolite of Fp, wherein the at least one metabolite is hypoxanthine. A use of the probiotic composition or hypoxanthine for manufacturing of a medicament for inhibiting the onset and progression of colorectal cancer.

The present application relates to the field of food processing technology, and provides a method and a device for purine reduction, and an equipment and an electronic equipment and a storage medium. The method for purine reduction includes the following steps: first cooling step: performing first cooling treatment on a food material to be treated at a first preset cooling rate to reduce a temperature of the food material to be treated to a temperature below a freezing point. By performing first cooling treatment on a food material to be treated at a first preset cooling rate to reduce a temperature of the food material to be treated to a temperature below a freezing point, the activity of ATP-degrading enzyme in the food material to be treated can be rapidly inhibited, which can quickly and effectively inhibit the generation of hypoxanthine, thereby reducing the generation of hypoxanthine, reducing the risk of hyperuricemia and gout, and having little effect on the taste and quality of the food material.

The present invention provides a therapeutic drug for frailty or muscular dystrophy, and particularly for sarcopenia, and a medicinal drug for improving motor function. The present invention provides a motor function improver that contains a compound represented by general formula (I) or a pharmaceutically acceptable salt thereof and additionally contains an ATP precursor substance or is used in combination with an ATP precursor substance. The ATP precursor substance is preferably inosine, inosinic acid, or hypoxanthine. This motor function improver is preferably used as a medicinal drug and is used as a therapy or preventive for frailty and sarcopenia and as a therapy or preventive for muscle wasting and muscle loss.

The application discloses a lung nodule metabolic marker and a screening method and a construction method and application of a screening model. Specifically disclosed are the application of metabolic markers and / or reagents for detecting the metabolic markers in the preparation of products for lung nodule screening; the metabolic markers include hypoxanthine, L-ornithine, decanoyl-L-carnitine, dehydroepiandrosterone sulfate and bethanidine. The application further discloses a method for constructing a lung nodule screening model. The metabolic markers and the lung nodule screening model constructed by using the metabolic markers can effectively screen out individuals with lung nodules from healthy people, have very high sensitivity and good specificity, are fast, accurate, non-invasive and non-radiation, are suitable for wide promotion, can be used as a pre-screening tool of LDCT, are used for the preliminary screening of lung nodules of a large-scale population, and have important significance for the early discovery of lung cancer and the improvement of the prognosis of patients.

The present invention discloses a use of a hypoxanthine compound in preparing a drug for treating pulmonary fibrosis and relates to the technical field of biological medicines. In the use of the hypoxanthine compound in preparing the drug for treating the pulmonary fibrosis, through pharmacological activity detection for treatment of pulmonary fibrosis with hypoxanthine compounds subjected to structural modification and transformation, the pharmacological activities of such the compounds in various animal disease models are tested and data about the activities of preventing and treating different types of pulmonary fibrosis are provided, which proves that the hypoxanthine compounds have good activities, with an effect being obviously superior to that of a positive drug Nintedanib commonly used in clinic, and that of hypoxanthine analogues A, B, C and D as well as other hypoxanthine analogues in the prior art. The use of the hypoxanthine compound in preparing the drug for treating the pulmonary fibrosis can provide a novel skeleton for screening novel compounds for preparing drugs for treating pulmonary fibrosis and lay a theoretical foundation of developing a novel lead compound.

The invention discloses a composite functional bacterial agent for regulating nucleoside substances in Luzhou-flavor liquor as well as a preparation method and application of the composite functional bacterial agent, and belongs to the technical field of liquor production. The compound functional bacterial agent is prepared from a liquid culture of pichia kudriavzevii GTY837 and a liquid culture of pichia kudriavzevii PKW194, or a liquid culture of pichia kudriavzevii GTY837 and a liquid culture of pichia kudriavzevii PKW194, or a liquid culture of pichia kudriavzevii PKW194, or a liquid culture of pichia kudriavzevii GTY837 and a liquid culture of pichia kudriavzevii PKW194. According to the composite functional bacterial agent provided by the invention, through the synergistic effect of the GTY837 and the PKW194, the content of seven nucleoside substances such as cytidine and hypoxanthine in the Luzhou-flavor liquor is effectively increased. The microbial agent solves the technical problem of unstable generation of nucleoside substances in traditional fermentation, and realizes synergistic improvement of the flavor and health attribute of Baijiu by enriching nucleoside substances with the functions of dispelling the effects of alcohol, protecting nerves and the like while enhancing the mellow feeling, delicate flavor and other sensory qualities of the Baijiu.

This invention discloses *Pediococcus lactis* TC-4, which simultaneously degrades nucleosides, purines, and uric acid, and its applications. *Pediococcus lactis* ( Pediococcus acidilactici This invention relates to *Pediococcus lactis* TC-4, with accession number GDMCC No: 67936. *Pediococcus lactis* TC-4 of this invention can efficiently degrade three nucleosides (guanosine, adenosine, and inosine), three purines (guanine, xanthine, and hypoxanthine), and uric acid in vitro. *Pediococcus lactis* TC-4 of this invention can effectively reduce serum uric acid levels in broiler chickens, alleviating and treating hyperuricemia. Compared with traditional chemical drugs for treating hyperuricemia and / or gout, this invention has no toxic side effects on the ecological environment, no residue risk, and is green and safe. This invention has been confirmed at multiple levels, including genetic and animal studies, to have no potential harm to humans, demonstrating its advantages of safety and high efficiency.

The invention provides a method for simultaneously determining various flavor nucleotides in a livestock and poultry meat product and application, and the method comprises the following steps: freeze-drying the livestock and poultry meat product, and then preparing the freeze-dried livestock and poultry meat product into a test solution; measuring the contents of CMP (cytidylic acid), ATP (adenosine triphosphate), GMP (guanine monophosphate), IMP (inosinic acid), ADP (adenosine diphosphate), Hy (hypoxanthine), AMP (adenosine monophosphate) and IN (inosine) in the test solution by adopting HPLC (high performance liquid chromatography); in HPLC, a mobile phase A is a phosphate buffer solution with the volume concentration of 95%, a mobile phase B is methanol with the volume concentration of 5%, gradient elution is carried out, and the gradient elution condition is 0-11 min, 99% phosphate buffer solution and 1% methanol; when 12 min, 80% phosphate buffer solution and 20% methanol are added, and the conditions are maintained for 6-7 min; the phosphate buffer solution is heated to 99% within 2 minutes, and then the temperature is kept for 8-10 minutes.

The invention discloses a CHO cell culture medium additive formula and a culture method. The CHO cell culture medium additive formula can be used for an upstream stage of in-vitro CHO cell culture and can remarkably reduce the proportion of Man5 in antibody glycosylation. The additives comprise an additive G1, an additive G2 and an additive G3; the additive G1 is a mixed mother solution with the concentration of 5g / L prepared from uridine, cytidine and hypoxanthine; the additive G2 is a mixed mother solution with the concentration of 1g / L prepared from sodium selenite and cadmium sulfate; the additive G3 is a mixed mother solution with the concentration of 100g / L prepared from asparagine, glutathione and sodium pyruvate. Compared with the prior art, the serum-free culture medium for culturing CHO cells provided by the invention has the advantages of simple components, low cost, no protein and no serum. Under the condition that cell growth and expression are not affected, the Man5 proportion in antibody glycosylation can be remarkably reduced.

The invention discloses a detection method for nucleoside components of cuttlebone medicinal materials, decoction pieces, standard decoction or formula granules and application of the detection method, and relates to the technical field of traditional Chinese medicine identification. The detection method for nucleoside components in cuttlebone medicinal materials, decoction pieces, standard decoction or formula granules comprises the following steps: extracting cuttlebone medicinal materials, decoction pieces, standard decoction or formula granules by adopting an extraction solvent to obtain a test solution; dissolving or extracting a reference substance and / or a cuttlebone reference medicinal material by adopting an extraction solvent to obtain a reference substance solution and / or a reference medicinal material solution; determining the test solution, the reference substance solution and / or the reference medicinal material solution by adopting a liquid chromatograph; wherein a chromatographic column of the liquid chromatograph takes octadecylsilane chemically bonded silica as a stationary phase, methanol as a mobile phase A and water as a mobile phase B for gradient elution, and the reference substances comprise a uracil reference substance, a cytidine reference substance, a hypoxanthine reference substance, a thymine reference substance, a guanosine reference substance and an adenosine reference substance; the extraction solvent is water or a methanol aqueous solution with the concentration of 10-30 vol%. The detection method disclosed by the invention can be used for determining various nucleoside components in the cuttlebone, effectively identifying common counterfeit products and providing a scientific basis for formulating a quality standard of the cuttlebone.

The present invention relates to the technical field of biomedicine and discloses use of a hypoxanthine derivative in the preparation of a drug for treating cardiomyopathy and / or myocardial fibrosis. By assaying pharmacological activities of structurally modified and altered hypoxanthine derivatives in treating cardiomyopathy and / or myocardial fibrosis, the pharmacological activities of such compounds were tested in various animal disease models, and data on activities for preventing and treating different types of cardiomyopathy and / or myocardial fibrosis were provided. It is proven that the hypoxanthine derivatives all have good activities, the effects are significantly superior to those of a clinically commonly used drug, nintedanib, and the effects are also significantly superior to those of hypoxanthine derivatives A, B, and C and other hypoxanthine derivatives in the prior art. The hypoxanthine derivative of the present invention can provide a novel skeleton for the screening of new compounds for the preparation of a drug for treating cardiomyopathy and / or myocardial fibrosis.

To provide a screening method for a human Na+ dependent hypoxanthine transporter inhibitor to be a candidate substance for an agent for treating or preventing chronic kidney disease, particularly xanthinuria.SOLUTION: The screening method includes the step for bringing a cell expressing human SLC23A3 (solute carrier family 23 member 3) gene product into contact with a test substance in the presence of hypoxanthine and sodium ions.SELECTED DRAWING: None

The present application belongs to the field of biological sequencing technology, and discloses a MIP probe and its application in urine free DNA capture and library construction. The MIP probe includes, from 5'-3', a 5' capture arm, an F-terminal adapter containing 10nt hypoxanthine, an R-terminal adapter, and a 3' capture arm. The present method prepares a DNA library by complementary base pairing, extension, and connection of the capture arm, is compatible with DNA fragments of different lengths and single-stranded DNA, improves template utilization, and thus facilitates the detection of low-frequency mutations in urine free DNA. The present method fixes the length of the library insert fragment by controlling the span length of the chromosomal DNA to be tested between the capture arms on both sides of the probe, making the DNBSEQ platform sequencing fluorescence signal more uniform, and the data output and quality higher. The present method combines the three steps of capture, library construction, and machine library preparation, eliminating the operations such as intermediate library quality control and pre-capture drying and concentration in conventional methods, and the operation time is shorter; there is no PCR amplification during the capture and library construction process of the present method, which reduces the error accumulation due to PCR amplification, reduces background noise, and improves the sensitivity and specificity of low-frequency mutation detection.

The embodiment of the invention discloses a primer probe combination for detecting TTV:: RARA fusion genes by a real-time fluorescent PCR method, a kit and application. According to the invention, it is found for the first time that the UTR region of TTV is a necessary element for formation and effective detection of the TTV:: RARA fusion gene, and the UTR sequence has high conservative property among different TTV strains. Based on the key characteristic, a specific detection primer is specifically designed. Wherein the upstream primer TTV:: RARA-F adopts a degenerate primer design strategy, hypoxanthine (I basic group) is introduced, the design can cover TTV strains with different genome sequences in the body of an APL patient, broad-spectrum detection of multiple TTV:: RARA fusion genes is realized, expression products of the fusion genes can be specifically recognized, the advantages of detection efficiency and accuracy are achieved, and the kit has the advantages of high specificity, high sensitivity, high sensitivity and the like. The detection period is effectively shortened and the detection accuracy is improved.

A polyphenol additive for improving the frozen storage quality of unwashed surimi and its application. The polyphenol additive for improving the frozen storage quality of unwashed surimi has active ingredients including polyphenols that can inhibit the activity of acid phosphatase, polyphenols that can inhibit the activity of adenosine deaminase, and polyphenols that can inhibit the activity of xanthine oxidase. The present invention also includes the application of the polyphenol additive for improving the frozen storage quality of unwashed surimi. The present invention can effectively reduce the contents of inosine and hypoxanthine in frozen unwashed surimi, delay the increase of K value, and improve the gel strength of frozen unwashed surimi. The usage method is simple and the application range is wide. It is derived from edible plants and has high safety. The product has low cost, simple processing technology, and is suitable for industrial production.

The application discloses a bird's nest peptide for inhibiting xanthine oxidase and application thereof, and belongs to the technical field of functional polypeptides. The amino acid sequence of the bird's nest peptide for inhibiting xanthine oxidase is WGLL, can chelate an active center of xanthine oxidase (XOD), and is helpful for inhibiting the activity of XOD in catalyzing conversion of xanthine or hypoxanthine. The application further provides a bird's nest peptide powder containing the bird's nest peptide for inhibiting xanthine oxidase. The bird's nest peptide powder can significantly reduce the rate of conversion of xanthine or hypoxanthine into uric acid catalyzed by XOD, has good uric acid reducing effect, can be applied to development of uric acid reducing products or various uric acid reducing products, and has high market value.

A dual-modal quantitative detection method for hypoxanthine to determine meat freshness includes the following steps: Step 1: Measuring the RGB values ​​of the color signal after the sample reaction is complete; Step 2: Measuring the temperature T of the reaction solution; Step 3: Constructing a linear equation between hypoxanthine concentration and color signal B / G or temperature T; Obtaining the color signal B / G based on a series of standard tests at different concentrations, and constructing the linear equation B / G = XC. Hx +y, where C Hx The concentration of the standard is given; based on the temperature T obtained from the testing of a series of standard concentrations, a linear equation is constructed: T = XC. Hx +y, where C Hx For the standard concentration; Step 4: Take the sample to be tested and repeat steps 1 and 2 to obtain the color signal B / G. n and temperature T n B / G n and T n Substituting the values ​​into the corresponding linear equation, the concentration of hypoxanthine in the sample is obtained. This invention employs a colorimetric-photothermal dual-modal detection method, which improves the reliability of the detection.

The invention relates to metabonomic characterization of microorganisms, in particular to a method for identifying cell types of cells in a sample, comprising: culturing the sample in a growth medium comprising nicotinamide to obtain a cultured growth medium; analyzing the cultured growth medium by chemical analysis; and identifying the cell type as at least one of the genus Escherichia, the genus Klebsiella, the genus Pseudomonas, the genus Enterococcus, the genus Staphylococcus or the genus Streptococcus when the cultured growth medium contains a higher concentration of nicotinate than the growth medium; wherein the growth culture medium comprises the following components in a buffer culture medium: 0.02 to 2 g / l of D-glucose, 0.0001 to 0.1 g / l of nicotinamide, 0.0001 to 0.1 g / l of pyridoxin.HCl, 0.02 to 1 g / l of hypoxanthine, 0.2 to 5 g / l of L-arginine, 0.2 to 5 g / l of L-threonine, 0.1 to 5 g / l of L-histidine, 0.02 to 2 g / l of spermine and catalase. According to the method, identification and toxin sensitivity detection can be completed within about half of time compared with that of an existing method.

The invention relates to a divisional application with the application number of 202411710591.1, discloses a marker for diagnosis or prognosis evaluation of children with epilepsy as well as a detection method and application of the marker, and belongs to the field of detection. The marker comprises one or more of the following purine metabolites: uric acid, creatinine, xanthine, hypoxanthine, guanine, adenine, adenosine, adenosine triphosphate, adenosine diphosphate and adenylic acid. The difference of blood / urine purine metabolite concentrations of an epilepsy child and a healthy control is reported, a disease prediction model based on the difference of various indexes of blood / urine purine is constructed, a comprehensive scoring algorithm is established, a detection result is convenient to interpret, and a clinical basis is provided for diagnosis and prognosis evaluation of child epileptic seizure.

This invention provides an enzymatic method for synthesizing hypoxanthine, comprising the following steps: dissolving adenine in an appropriate amount of water, adding an effective amount of deaminase, incubating the reaction at 30-50°C, adjusting the pH to 6.5-7 with acid after the reaction, and filtering and washing with water to obtain hypoxanthine. This invention uses adenine as a raw material and employs a deaminase method to synthesize hypoxanthine, overcoming the shortcomings of natural raw material extraction methods, such as complex extraction processes, low yields, and high costs. Compared with other chemical synthesis methods, it produces no byproducts and has higher yields and purity, making it highly valuable for widespread application.