39 results about "Tetrazolium chloride" patented technology

The invention discloses a synthesis method of chloridized-2,3,5-triphenyl tetrazolium chloride. The synthesis method comprises the following steps: (1) carrying out a reaction on benzaldehyde and phenylhydrazine so as to synthesize benzaldehyde-phenylhydrazone; (2) adding phenylamine into a hydrochloric acid and dripping a sodium nitrite aqueous solution at a low temperature so as to prepare a diazonium salt; (3) dripping the diazonium salt solution obtained in the step (2) into the benzaldehyde-phenylhydrazone solution obtained in the step (1) so as to synthesize 1,3,5-triphenyl formazan; and (4) dissolving the 1,3,5-triphenyl formazan obtained in the step (3) in methyl alcohol and carrying out an oxidation reaction so as to synthesize the chloridized-2,3,5-triphenyl tetrazolium chloride. The method has the characteristics that the process is simple; an oxidizing agent with high efficiency and low toxicity is adopted; the yield and the purity of the chloridized-2,3,5-triphenyl tetrazolium chloride are relatively high.

The invention discloses a method for preparing and preserving a high-purity culture of ralstonia solanacearum causing tobacco bacterial wilt. The method provided by the invention comprises the following steps of: dealing with stalks of diseased or infected plants with tobacco bacterial wilt typical symptoms and then keeping the humidity at 27-30 degrees centigrade at a sterile state for 12-24 hours to obtain a culture containing ralstonia solanacearum; identifying and sieving a high-purity culture with the bacteria-containing amount of more than 80% by using a TTC (Triphenyl-tetrazolium chloride) method; and preserving the obtained high-purity culture at the constant temperature from minus 20 degrees centigrade to minus 40 degrees centigrade by utilizing a preserving fluid A or preservingthe obtained high-purity culture at the constant temperature of 20 degrees centigrade by utilizing a preserving fluid B, wherein the preserving fluid A is composed of 100-200 mL of glycerol and 20-40g of skim milk powder and is prepared by the following steps of: adding distilled water to dissolve and determine the volume to 500 mL; shaking up and regularly sterilizing to prepare the preserving fluid A, and the preserving fluid B is prepared by the following steps of: adding 1-2 g of agar into 1000 mL of distilled water to be melted; shaking up and regularly sterilizing to prepare the preserving fluid B. The method for preparing and preserving the high-purity culture of ralstonia solanacearum causing tobacco bacterial wilt, provided by the invention, is simple and feasible and has the advantages of high purity of the target bacteria, stable performance and good preserving effect.

The invention relates to a method and a kit for setting the survival rate of nematode quickly. In the method, the nematode is dyed by using a dye which is 2,3,5-triphenyl tetrazolium chloride (TTC); live nematode can be dyed, dead nematode cannot be dyed, and a light absorption value is in proportion to the number of the live nematode, so the survival rate of the nematode can be reflected directly, and the kit is prepared according to the survival rate. The method and the kit can be applied in aspects of medicinal screening, pesticide screening, environmental evaluation, water quality evaluation which take the survival rate of the nematode as indexes. The method and the kit are quick, simple and easy in operation, low in requirement on equipment and low in cost, and have a high application value.

The invention belongs to the application field of plant physiology and biochemistry technology. In particular, it relates to a method and application for rapid quantification of pollen activity at normal temperature or high temperature. The formula, dyeing concentration, optimum dyeing time and scope of application of pollen TTC dyeing solution at room temperature and high temperature were determined, and the formula of combined dyeing solution was prepared: 1mol / L potassium dihydrogen phosphate 5.23g, dipotassium hydrogen phosphate 14.03g, pH 7 potassium phosphate buffer solution; then 8g of 2,3,5-triphenyltetrazolium chloride (TTC) was dissolved in the resulting potassium phosphate buffer solution, and the volume was adjusted to 1L with distilled water, so that the final concentration was 8g / L. The invention can be used for quantitative detection of pollen activity of large batches of materials, omits the microscopic inspection operation after dyeing, simplifies the process of identification of pollen activity of large batches of materials, and is suitable for identification of pollen activity of various crops such as cotton and rice.