39 results about "Tetrazolium chloride" patented technology

The invention relates to a kit for detecting food-borne pathogenic bacteria, which comprises 12 groups of primers and probes thereof, wherein in the primers, the 5' ends of downstream primers are all tagged with biotin. The kit amplifies nucleic acid in a sample to be detected by utilizing the primers, and a formed biotinylated product and a probe fixed on the surface of a chip are hybridized to form a probe-biotinylated product compound; an alkaline phosphatase-streptavidin conjugate is combined with the probe-biotinylated product compound, and then chromogenic substrate which is tetranitroblue tetrazolium chloride / 5-bromine-4-chlorine-3-indolyl-phosphate (NBT / BCIP) is reacted with alkaline phosphatase to undergo a colour generation (bluish violet) reaction; and whether the sample to be detected contains the pathogenic bacteria to be detected is detected by interpreting whether a specific site has colour generation. The kit has the advantages of high detection throughput, high speed, high sensitivity, high specificity and great practicability. The invention also provides the method for detecting the food-borne pathogenic bacteria by adopting the detection kit.

The invention belongs to the field of medical dressings, and particularly relates to color-developing medical dressing and a preparation method thereof. The preparation method comprises the following steps: taking TTC (Triphenyl Tetrazolium Chloride) as a color developing agent, mesoporous silica nanoparticles as a carrier and alginate and sodium carboxymethyl cellulose as spinning raw materials, preparing fiber containing color developing components through electrostatic spinning, and then preparing the fiber into the medical dressing containing the color developing components through a non-woven fabric technology. The color-developing medical dressing prepared by the preparation method has very good biological safety, and has an indicating function on a bacteria-infected wound; meanwhile, the color-developing medical dressing has very good liquid absorptivity, and is capable of absorbing leakage liquid of the wound and promoting the healing of the wound.

A method of quickly identifying bacterial wilt resistance in tobacco varieties in seedling stage comprises the main steps of A, raising seedlings of a tobacco variety under test in nutrient pots according to a floating seedling raising method; B, subjecting tobacco plants infected by bacterial wilt and collected from a field to isolation purification culture for 3-5 days via a TTC (triphenyl tetrazolium chloride) medium to obtain purified Ralstonia solanacearum; inoculating the Ralstonia solanacearum subjected to purification culture to NA (nutrient agar) medium, and culturing for 12 h to obtain an inoculation bacterial liquid; C, sucking the inoculation bacterial liquid with an injector, selecting tobacco seedlings growing with 3-4 true leaves in the nutrient pots in step A, and injecting 2-4 mu L of the inoculation bacterial liquid obliquely into a stalk of each selected tobacco seedling from its axil; D, culturing the inoculated tobacco seedlings in a sunlight culture room for 10 days, observing and recording disease attack conditions of the tobacco seedlings on 10th day of culture, calculating a disease index, and judging the bacterial wilt resistance of the tobacco variety under test. The method is simple to perform, high in measuring speed, good in results accuracy and good in result uniformity.

The invention relates to a method and a kit for setting the survival rate of nematode quickly. In the method, the nematode is dyed by using a dye which is 2,3,5-triphenyl tetrazolium chloride (TTC); live nematode can be dyed, dead nematode cannot be dyed, and a light absorption value is in proportion to the number of the live nematode, so the survival rate of the nematode can be reflected directly, and the kit is prepared according to the survival rate. The method and the kit can be applied in aspects of medicinal screening, pesticide screening, environmental evaluation, water quality evaluation which take the survival rate of the nematode as indexes. The method and the kit are quick, simple and easy in operation, low in requirement on equipment and low in cost, and have a high application value.

The invention relates to a method for rapidly screening thermophilic anaerobic ethanol microbe high-yield ethanol bacterial strain, comprising the following steps: 1) wild strains are cultured continuously in a fluid nutrient medium containing ethanol, the ethanol tolerance of the thermophilic anaerobic ethanol microbe is evolved directionally; 2) ethylmethylsulfone mutagenesis is carried out to ethanol-tolerance bacterial strains, and the mutagenesis bacterium liquid is coated on the solid culture medium containing the ethanol; 3) after individual bacterial colony is formed, the individual bacterial colony with large radius is dropwise added in 2,3,5-triphenyl chlorination tetrazole developing liquid, and single spots with deep color development are taken for re-screening on a CaCO3 rescreening plate containing high substrate concentration; 4) bacterial colonies with large radius and small radius of a transparent ring, pH indicators are dropwise added for eliminating false positive; 5) the ethanol concentration is tested by a gas chromatography or an ethanol quantitative kit after fermentation is carried out, and the bacterial strains with high ethanol output are cultured for at least 20 generations under a non-selection condition, and the bacterial strain which still has high yield is destination bacterial strain.

The invention belongs to the technical field of seed detection, and in particular relates to a method for rapidly detecting viability of aeluropus littoralis seeds. The method is realized through the following steps of pre-soaking the aeluropus littoralis seeds in ozone water and aqueous solution containing alkyl glycoside and fatty acid methyl ester ethoxylate, then, dyeing one ends of the aeluropus littoralis seeds having seed embryos through mixed solution of TTC (Triphenyl Tetrazolium Chloride) solution and zinc gluconate solution, soaking the dyed seeds by using a lactic acid phenol transparent agent, observing the dyeing condition of the aeluropus littoralis seeds, and calculating the percentage of viability seeds. The method provided by the invention is rapid and effective; the soaking time can be shortened; the mechanical injury to seeds can be reduced; and the method provided by the invention has important practical significance on pasture utilization and ecological protection.

The invention relates to the technical field of pseudomonas aeruginosa detection and discloses a pseudomonas aeruginosa chromogenic culture medium and a method for quick detection by the same. The pseudomonas aeruginosa chromogenic culture medium comprises a basic medium, a chromogenic substrate and an antibacterial agent. The chromogenic substrate comprises 2,3,5-phenyl tetrazolium chloride, 4-methyl umbelliferone-D-glucuronide, and the antibacterial agent comprises nalidixic acid, cycloserine and bile salt. 2,3,5-phenyl tetrazolium chloride acts on a pseudomonas aeruginosa metabolite to showa red color; 4-methyl umbelliferone-D-glucuronide acts on pseudomonas aeruginosa to show fluorescence under a 366nm ultraviolet lamp; by joint action of 2,3,5-phenyl tetrazolium chloride and the antibacterial agent, growth of common disturbance bacteria can be inhibited, and false positive results are avoided. By adoption of the pseudomonas aeruginosa quick detection method, detection and count of pseudomonas aeruginosa can be finished in 24h, and time saving, labor saving, stability, reliability and high sensitivity are realized.

The invention belongs to the field of application of plant physiological and biochemical technology, and specifically relates to a method for rapid quantification of pollen activity at normal temperature or high temperature, and application. The method comprises the steps of determining formulations, dyeing concentrations, optimum dyeing times and applicable ranges of pollen TTC staining solutionat a normal temperature and a high temperature, preparing a combined dyeing solution formula: 5.23g of 1 mol / L potassium dihydrogen phosphate, 14.03g of dipotassium hydrogen phosphate and potassium phosphate buffer with pH of 7; dissolving 8g of 2,3,5-triphenyltetrazolium chloride (TTC) in the obtained potassium phosphate buffer, and adjusting the volume to 1L with distilled water to a final concentration of 8g / L. The invention can be used for quantitative detection of pollen activity of large quantities of materials, omits the microscopic examination operation after dyeing and simplifies theprocess of pollen activity identification of large quantities of materials, which is suitable for pollen activity identification of cotton, rice and other crops.

The invention provides an active oxygen quantitative detection kit. The kit is prepared from Nitro blue tetrazolium chloride monohydrate freeze-dried powder, a blue tetrazolium chloride monohydrate solution, a color developing agent A, a color developing agent B and a standard substance, wherein the chlorinated nitro tetrazole blue freeze-dried powder is powder obtained by freeze-drying blue tetrazolium chloride monohydrate liquid with a concentration of 0.1-1mmol / L; the blue tetrazolium chloride monohydrate solution is a phosphate buffer solution which has a concentration of 0.01-0.5mol / L anda pH value of 7.2-7.6; the color developing agent A is a weak acid buffer solution with a concentration of 0.01-0.3mol / L; the color developing agent B is 0.01-2% triethanolamine solution; the standard substance contains a hydrogen peroxide solution with a known concentration of 0-36mmol / L. The kit provided by the invention has the advantages that the method is scientific, clean and environmentally-friendly, manual operation and automatic batch inspection can be realized, and the kit is simple to operate and easy in clinical promotion.

A method to propagate, enumerate and quantify bacteriophage(s) in a water sample or other aqueous sample was designed which contains ingredients to stimulate the growth of select bacterial species which are susceptible to infection by specific bacteriophage(s), in which interfering background organisms are either inhibited or inconsequential. Important features of the medium include oxidation-reduction compounds producing colored and / or fluorescent products, chromogenic and / or fluorogenic enzyme substrates, and temperature-independent gelling agent(s). A preferred combination is the growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and appropriate gelling agents, which (when properly used) produces a dark red bacterial lawn containing teal blue-green, irregularly circular spots representing individual phage plaque, all discernible to the eye in visible light. The procedure can also be readily applied towards automatic counting systems under artificial illumination. The procedure can be employed with water samples and with elution buffers that can retain bacteriophages in suspension following contact by the buffer with foods, soils, hard surfaces and other solids that may be contaminated by bacteriophages.

The invention relates to a method for determining vitality of pseudo-ginseng pollen. The method comprises the following steps: using a cane sugar solution and a 2,3,5-triphenyl tetrazolium chloride solution to prepare a dyeing reaction solution; dropping the dyeing reaction solution on a cover glass, selecting the pseudo-ginseng pollen in the dyeing reaction solution on the cover glass, overturning the cover glass and placing the cover glass on a concave slide glass; placing the pollen in a thermotank for culturing; taking the pollen out and placing the pollen under a microscope for observation, selecting the visual field at random, and counting the proportion of dyed pollen grain number and the undyed pollen grain number, and wherein the pseudo-ginseng pollen with 30% or more has vitality. The method has the beneficial effects of simple operation, convenient and practical performances, high accuracy, and reliable statistics data, and has high practical application value, and has active promotion effect for breeding efficiency, output and quality of pseudo-ginseng.

The invention discloses a method for preparing and preserving a high-purity culture of ralstonia solanacearum causing tobacco bacterial wilt. The method provided by the invention comprises the following steps of: dealing with stalks of diseased or infected plants with tobacco bacterial wilt typical symptoms and then keeping the humidity at 27-30 degrees centigrade at a sterile state for 12-24 hours to obtain a culture containing ralstonia solanacearum; identifying and sieving a high-purity culture with the bacteria-containing amount of more than 80% by using a TTC (Triphenyl-tetrazolium chloride) method; and preserving the obtained high-purity culture at the constant temperature from minus 20 degrees centigrade to minus 40 degrees centigrade by utilizing a preserving fluid A or preservingthe obtained high-purity culture at the constant temperature of 20 degrees centigrade by utilizing a preserving fluid B, wherein the preserving fluid A is composed of 100-200 mL of glycerol and 20-40g of skim milk powder and is prepared by the following steps of: adding distilled water to dissolve and determine the volume to 500 mL; shaking up and regularly sterilizing to prepare the preserving fluid A, and the preserving fluid B is prepared by the following steps of: adding 1-2 g of agar into 1000 mL of distilled water to be melted; shaking up and regularly sterilizing to prepare the preserving fluid B. The method for preparing and preserving the high-purity culture of ralstonia solanacearum causing tobacco bacterial wilt, provided by the invention, is simple and feasible and has the advantages of high purity of the target bacteria, stable performance and good preserving effect.

The invention provides a method for quantitatively determining viability of barley seeds, belongs to the technical field of evaluation of the viability of the barley seeds, and can evaluate germination potential of the barley seeds rapidly and stably and provide reference for seed purchase and production. According to the method, TTC (triphenyl tetrazolium chloride) is used for dyeing dissected barley seeds, the dyed barley seeds are crushed with a freeze-drying and ball-milling crushing combined technique, a 10% trichloroacetic acid methanol solution is adopted as extract liquor to perform extraction on the crushed barley seeds, TTF (1,3,5-triphenylformazan) is obtained, and content of TTF is analyzed quantitatively according to a TTF standard curve with absorption photometry to predict the viability of the barley seeds. With the adoption of the freeze-drying and ball-milling crushing combined technique, loss of a sample in an operation process is reduced, and an experimental result is more precise and more reliable. By separating and dissecting the barley seeds, interference of useless substances in follow-up experiments is reduced, reaction volume is reduced, and experiment costand workload are saved.

The invention relates to the field of agricultural product detection, and in particular relates to a method for detecting cell viability of fresh Cordyceps sinensis. The method is mainly as follows: after pretreatment, the fresh Cordyceps sinensis is sliced, slices are placed into a small beaker with a certain concentration of a 2, 3, 5-triphenyl tetrazolium chloride solution for soaking, after light-proof thermostatic waterbath dyeing for a period of time, the dyed fresh Cordyceps sinensis slices are taken out, surface water is absorbed to dry, the dying condition of the fresh Cordyceps sinensis slices is observed, and according to color depth and dyeing uniformity, the cell viability of fresh Cordyceps sinensis can be determined. The method is a method for quickly, conveniently and accurately detecting the cell viability of the fresh Cordyceps sinensis.

The invention relates to a preparation method of a biochemical detection reagent of chlorinated tetrazolium salts. In the preparation method, formazan is used as a reactant and is oxidized into a chlorinated tetrazolium salt by adopting a hydrogen chloride / hydrogen peroxide system. The preparation method specifically comprises the following steps of: 1) dissolving formazan into the concentrated hydrochloric acid / methanol mixed liquid, 2) dropwise adding aqueous hydrogen peroxide solution under the condition that the dropwise adding temperature range is minus 10 DEG C to 15 DEG C, and stirring at the room temperature after dropwise adding, and 3) removing methanol by rotary evaporation, extracting with water-insoluble organic solvent and drying, adding anhydrous ethyl ether to separate out a crude product, and recrystallizing the mixed solvent to obtain the chlorinated tetrazolium salt refined product. The preparation method provided by the invention has the advantages of improving the yield at last, being simple in treatment, having no heavy metal residual in the product and being green in the preparation process.

The invention belongs to the field of microbial detection and particularly discloses a method for quantitatively detecting molds and saccharomycetes in eye masks; the method comprises the steps of (1) weighing eye mask, and adding diluent of suitable volume to obtain a test liquid; (2) adding a mold liquid medium of suitable volume into the test liquid, and performing enrichment culture at 28+ / - DEG C for 48-72 h to obtain homogenous liquid; (3) collecting a suitable amount of the homogenous liquid, applying respectively to rose Bengal agar plate and triphenyl tetrazolium chloride-Sabouraud agar plate, standing, and culturing at 28+ / -1 DEG C for 3-5 days; (4) judging results, to be specific, if no colonies grow on the plates or if there are colonies grown on the plates while no suspected colonies are present, determining zero match; if it is confirmed that saccharomycetes or molds grown on any one of the plates, determining that a target bacterium is detected on the sample. The method has the advantages of high specificity, low detection limit, good reproducibility, good operational convenience and the like, and meets the requirement of manufacturers for product quality control.

The invention relates to the technical field of analytic chemistry detection, in particular to a quick detection method of hydrocortisone. The quick detection method includes: taking a to-be-detected sample, adding a hydrochloric solution, shaking, adding ethyl acetate, shaking, and centrifuging or standing for layering; taking centrifugate or upper-layer solution, adding a sodium hydroxide solution, shaking, and centrifuging or standing for layering; dripping the sodium hydroxide solution onto a white drip plate, dropwise adding a triphenyl tetrazolium chloride test solution, mixing well, dropwise adding a test sample solution, and observing color change. The quick detection method is simple and convenient to operate and low in analysis cost; interference of related factors is eliminated, and observation of solution color is facilitated better; extracting liquid, washing liquid, leaching liquid and a detection agent which are used in the method are high in specificity; interference substances are separated and removed by utilizing dissolubility difference of different substances for three times in the process of extracting, washing and leaching, so that sensitivity of chromogenic reaction is improved, detection result accuracy is high, the chromogenic reaction is quick and obvious in phenomenon, and the quick detection method is suitable for on-site quick detection.

The invention relates to the technical field of analytical chemical detection, in particular to a quick detection method of dexamethasone; comprising the steps of taking a sample to be detected adding hydrochloric acid solution, shaking, adding ethyl acetate, shaking, and centrifuging or standing to allow layering; taking supernate or upper solution, adding sodium hydroxide solution, shaking, and centrifuging or standing to allow layering; dropping sodium hydroxide solution to a white dropper plate, dropwise adding triphenyl tetrazolium chloride test solution, mixing well, dropwise adding test sample solution, and observing changes in color. The method of the invention is simple to perform and low in analytical cost; despite of the influence of related factors, the observation of solution color is more facilitated, the abstracting liquid, washing liquid, extracting liquid and detection agents used herein are highly specific, interfering substances are separated and removed by using dissolution differences of different substances three times in the abstracting, washing and extracting processes, the sensitivity of color reaction is improved, detection results accuracy is high, the color reaction is quick, phenomena are significant, and the method is suitable for quick field detection.

The invention relates to phenyltetrazolium chloride, and in particular, relates to a preparation method of phenyltetrazolium chloride. The preparation method comprises the steps: with benzaldehyde, aniline and phenylhydrazine as basic raw materials, synthesizing phenylhydrazone by benzaldehyde and phenylhydrazine firstly, then synthesizing triphenylformazan red crystals from diazonium salt formedby aniline, and then cyclizing into 2,3,5-triphenyltetrazolium chloride by using an organic catalyst. The process completely avoids use of lead tetraacetate as the catalyst to synthesize the product.Compared with a traditional process, the preparation method does not use lead tetraacetate, eliminates the influence of lead on the environment, and has the yield reaching 70% or more and the purity of more than or equal to 99% (HPLC).

The invention belongs to the technical field of online monitoring of safety of agricultural products and food products, and discloses a preparation method and a using method of a chilled fresh poultrystorage and transportation freshness safety warning card. The chilled fresh poultry storage and transportation freshness safety warning card is sealed in a container, and comprises filter paper for absorbing color rendering enrichment broth, and a sealed bacteria solution bag. The color rendering enrichment broth is prepared from a chicken extract, sodium chloride and 2, 3, 5-triphenyl tetrazolium chloride; the bacteria solution bag is filled with 102-103cfu / mL pseudomonas aeruginosa. When the chilled fresh poultry storage and transportation freshness safety warning card is used, the sealed bacteria-containing plastic bag in a packaging bag of the warning card is broken by kneading before chilled fresh poultry enters a finished product warehouse, and the warning card is fully wetted and maintained in a sealed state; the warning card is placed outside a packaging bag of the chilled fresh poultry and is transported together with the chilled fresh poultry. When purchasing the chilled fresh poultry, a consumer observes the color of the warning card so as to judge the freshness of the poultry.

The invention discloses a method for screening oil-producing filamentous fungi, and particularly relates to a triphenyl tetrazolium chloride PDA culture medium suitable for the oil-producing filamentous fungi. The culture medium is prepared by the following steps: preparing a potato agar culture medium; and adding triclosan and triphenyl tetrazolium chloride, so that the potato agar culture mediumcontains 5 mg / L triclosan and 0.5 g / L triphenyl tetrazolium chloride. The method for screening the oil-producing filamentous fungi comprises the following steps: taking soil in sterile water, performing oscillating for 1-3 hours, and diluting a bacterial liquid; and coating the triphenyl tetrazolium chloride PDA culture medium with the bacterial liquid, separating oil-producing filamentous fungiand / or oil-producing filamentous fungi spores, and performing purifying to obtain thalli. According to the invention, the culture medium is preferably prepared, the oil-producing filamentous fungi arescreened, and lipid extraction is carried out on the oil-producing filamentous fungi, so that the oil obtaining efficiency is improved.

The invention application discloses metal adhesive glue used for high temperature. The metal adhesive glue consists of the following components in parts by weight: 83 to 106 parts of polyvinyl acetate, 5 to 10 parts of volcanic, 0.5 to 0.8 parts of methylhexahydrophthalic anhydride, 41 to 53 parts of vinyl acetate, 25 to 35 parts of cycloaliphatic epoxide resin, 0.2 to 0.4 parts of antioxygen and 0.1 to 0.3 parts of accelerant, wherein the accelerant is prepared from the composition of 4 parts to 8 parts of Iodonitrotetrazolium and 15 parts to 23 parts of ethylene glycol solution. As an epoxy group of the cycloaliphatic epoxide resin is directly connected onto an alicyclic ring, and a compact rigid molecular structure can be formed, the heat stability of the glue can be enhanced; furthermore, by performing reasonable mass fraction preparation on the polyvinyl acetate and the vinyl acetate, the content of free formaldehyde in the scheme is reduced; as the volcanic is of a porous structure, molecules of the volcanic are compact and porous, have very powerful adsorption capacity, and are capable of adsorbing part of hazardous substances, therefore the environmental protective performance of the formula of the metal adhesive glue is further improved.

The invention relates to a method and a kit for setting the survival rate of nematode quickly. In the method, the nematode is dyed by using a dye which is 2,3,5-triphenyl tetrazolium chloride (TTC); live nematode can be dyed, dead nematode cannot be dyed, and a light absorption value is in proportion to the number of the live nematode, so the survival rate of the nematode can be reflected directly, and the kit is prepared according to the survival rate. The method and the kit can be applied in aspects of medicinal screening, pesticide screening, environmental evaluation, water quality evaluation which take the survival rate of the nematode as indexes. The method and the kit are quick, simple and easy in operation, low in requirement on equipment and low in cost, and have a high application value.

A method for increasing embryo activity of mallotus tetracoccus seeds after cryopreservation comprises the following steps of taking dried seeds stored at the low temperature of -18 DEG C to 20 DEG C out together with a closed container for storing the seeds; placing the seeds in the environment with the temperature of 15 DEG C and the relative humidity of 15% for 24 hours, and then taking out the seeds; placing the seeds in an incubator with the temperature of 45 DEG C and taking out after 6 hours of heat treatment; placing the seeds on a 1% agar medium after the temperature of the seeds return to room temperature, and then placing at a 20 DEG C culturing room for 24 hours; cutting seed coats and embryos of the treated seeds, immersing in a 1% triphenyl tetrazolium chloride (TTC) solution for 48 hours in the dark at 30 DEG C; taking out the soaked seeds and observing embryo staining conditions, wherein embryos with activity show a uniform red color. The method significantly enhances the embryo viability of the mallotus tetracoccus seeds stored at the low temperature for more than 5 years, and solves the problem of reduction of the embryo vitality of the mallotus tetracoccus seeds after cryopreservation. The treatment method is simple and easy without any danger and side effects.

The invention discloses a low-temperature dry preservation method for paphiopedilum pollen. The method comprises the following steps that within 3-5 days when a paphiopedilum flower is completely unfolded, an anther cap of the flower is opened by using a sterilized picking tool; a paphiopedilum pollen mass is picked to a clean and sterile container by using the sterilized picking tool, and the pollen mass is dehydrated under a sterile state; the dehydrated pollen mass is well sealed, placed in an environment with the temperature of 4 DEG C and preserved in a dark place. The preservation time of the paphiopedilum pollen mass preserved by using the method can reach 6-12 months, and after the pollen mass is subjected to re-warming and rewetting, by determining the pollen viability through a triphenyl tetrazolium chloride staining method, it is found that the viability of the pollen preserved by using the method is high, and the selfing or hybridization success rate is up to 90-95%. By means of the method, the barriers of cross pollination of types and plants of paphiopedilum in different blooming periods are overcome, a foundation is laid for the further development of the culture and industries of new varieties of Chinese paphiopedilum, and therefore, the method has great social benefits and economic benefits.

The invention belongs to the technical field of seed detection, and in particular relates to a method for rapidly detecting viability of aeluropus littoralis seeds. The method is realized through the following steps of pre-soaking the aeluropus littoralis seeds in ozone water and aqueous solution containing alkyl glycoside and fatty acid methyl ester ethoxylate, then, dyeing one ends of the aeluropus littoralis seeds having seed embryos through mixed solution of TTC (Triphenyl Tetrazolium Chloride) solution and zinc gluconate solution, soaking the dyed seeds by using a lactic acid phenol transparent agent, observing the dyeing condition of the aeluropus littoralis seeds, and calculating the percentage of viability seeds. The method provided by the invention is rapid and effective; the soaking time can be shortened; the mechanical injury to seeds can be reduced; and the method provided by the invention has important practical significance on pasture utilization and ecological protection.

A method for increasing embryo activity of mallotus tetracoccus seeds after cryopreservation comprises the following steps of taking dried seeds stored at the low temperature of -18 DEG C to 20 DEG C out together with a closed container for storing the seeds; placing the seeds in the environment with the temperature of 15 DEG C and the relative humidity of 15% for 24 hours, and then taking out the seeds; placing the seeds in an incubator with the temperature of 45 DEG C and taking out after 6 hours of heat treatment; placing the seeds on a 1% agar medium after the temperature of the seeds return to room temperature, and then placing at a 20 DEG C culturing room for 24 hours; cutting seed coats and embryos of the treated seeds, immersing in a 1% triphenyl tetrazolium chloride (TTC) solution for 48 hours in the dark at 30 DEG C; taking out the soaked seeds and observing embryo staining conditions, wherein embryos with activity show a uniform red color. The method significantly enhances the embryo viability of the mallotus tetracoccus seeds stored at the low temperature for more than 5 years, and solves the problem of reduction of the embryo vitality of the mallotus tetracoccus seeds after cryopreservation. The treatment method is simple and easy without any danger and side effects.

A method of detecting microorganisms in blood is provided. The method includes (1) a step of directly adding 2,3,5-triphenyltetrazolium chloride (TTC) into a blood culture flask, or a step of mixing 2,3,5-triphenyltetrazolium chloride into a silicone rubber oxygen permeable membrane to obtain a solid indicator and fixing the solid indicator to the bottom of the blood culture flask, with the concentration of the TTC being 0.01-0.03 g / mL, (2) a step of adding a blood medium into the blood culture flask, and sterilizing at 121 DEG C for 20 min, (3) a step of adding a blood sample into the blood culture flask, and culturing at 35 DEG C for 0-5 days, and (4) a step of detecting intensity of reflected light of an index strip in the blood culture flask, mapping an intensity curve of the reflected light, and determining a microorganism growth result according to the curve. The method is advantaged by low costs, simple operation processes, short detection time, high positive detection rates, and the like in clinical application, and is high in application value.