39 results about "Tetrazolium chloride" patented technology

The invention relates to a kit for detecting food-borne pathogenic bacteria, which comprises 12 groups of primers and probes thereof, wherein in the primers, the 5' ends of downstream primers are all tagged with biotin. The kit amplifies nucleic acid in a sample to be detected by utilizing the primers, and a formed biotinylated product and a probe fixed on the surface of a chip are hybridized to form a probe-biotinylated product compound; an alkaline phosphatase-streptavidin conjugate is combined with the probe-biotinylated product compound, and then chromogenic substrate which is tetranitroblue tetrazolium chloride/5-bromine-4-chlorine-3-indolyl-phosphate (NBT/BCIP) is reacted with alkaline phosphatase to undergo a colour generation (bluish violet) reaction; and whether the sample to be detected contains the pathogenic bacteria to be detected is detected by interpreting whether a specific site has colour generation. The kit has the advantages of high detection throughput, high speed, high sensitivity, high specificity and great practicability. The invention also provides the method for detecting the food-borne pathogenic bacteria by adopting the detection kit.

The invention belongs to the field of medical dressings, and particularly relates to color-developing medical dressing and a preparation method thereof. The preparation method comprises the following steps: taking TTC (Triphenyl Tetrazolium Chloride) as a color developing agent, mesoporous silica nanoparticles as a carrier and alginate and sodium carboxymethyl cellulose as spinning raw materials, preparing fiber containing color developing components through electrostatic spinning, and then preparing the fiber into the medical dressing containing the color developing components through a non-woven fabric technology. The color-developing medical dressing prepared by the preparation method has very good biological safety, and has an indicating function on a bacteria-infected wound; meanwhile, the color-developing medical dressing has very good liquid absorptivity, and is capable of absorbing leakage liquid of the wound and promoting the healing of the wound.

A method of quickly identifying bacterial wilt resistance in tobacco varieties in seedling stage comprises the main steps of A, raising seedlings of a tobacco variety under test in nutrient pots according to a floating seedling raising method; B, subjecting tobacco plants infected by bacterial wilt and collected from a field to isolation purification culture for 3-5 days via a TTC (triphenyl tetrazolium chloride) medium to obtain purified Ralstonia solanacearum; inoculating the Ralstonia solanacearum subjected to purification culture to NA (nutrient agar) medium, and culturing for 12 h to obtain an inoculation bacterial liquid; C, sucking the inoculation bacterial liquid with an injector, selecting tobacco seedlings growing with 3-4 true leaves in the nutrient pots in step A, and injecting 2-4 mu L of the inoculation bacterial liquid obliquely into a stalk of each selected tobacco seedling from its axil; D, culturing the inoculated tobacco seedlings in a sunlight culture room for 10 days, observing and recording disease attack conditions of the tobacco seedlings on 10th day of culture, calculating a disease index, and judging the bacterial wilt resistance of the tobacco variety under test. The method is simple to perform, high in measuring speed, good in results accuracy and good in result uniformity.

The invention relates to a method for rapidly screening thermophilic anaerobic ethanol microbe high-yield ethanol bacterial strain, comprising the following steps: 1) wild strains are cultured continuously in a fluid nutrient medium containing ethanol, the ethanol tolerance of the thermophilic anaerobic ethanol microbe is evolved directionally; 2) ethylmethylsulfone mutagenesis is carried out to ethanol-tolerance bacterial strains, and the mutagenesis bacterium liquid is coated on the solid culture medium containing the ethanol; 3) after individual bacterial colony is formed, the individual bacterial colony with large radius is dropwise added in 2,3,5-triphenyl chlorination tetrazole developing liquid, and single spots with deep color development are taken for re-screening on a CaCO3 rescreening plate containing high substrate concentration; 4) bacterial colonies with large radius and small radius of a transparent ring, pH indicators are dropwise added for eliminating false positive; 5) the ethanol concentration is tested by a gas chromatography or an ethanol quantitative kit after fermentation is carried out, and the bacterial strains with high ethanol output are cultured for at least 20 generations under a non-selection condition, and the bacterial strain which still has high yield is destination bacterial strain.

The invention belongs to the field of application of plant physiological and biochemical technology, and specifically relates to a method for rapid quantification of pollen activity at normal temperature or high temperature, and application. The method comprises the steps of determining formulations, dyeing concentrations, optimum dyeing times and applicable ranges of pollen TTC staining solutionat a normal temperature and a high temperature, preparing a combined dyeing solution formula: 5.23g of 1 mol/L potassium dihydrogen phosphate, 14.03g of dipotassium hydrogen phosphate and potassium phosphate buffer with pH of 7; dissolving 8g of 2,3,5-triphenyltetrazolium chloride (TTC) in the obtained potassium phosphate buffer, and adjusting the volume to 1L with distilled water to a final concentration of 8g/L. The invention can be used for quantitative detection of pollen activity of large quantities of materials, omits the microscopic examination operation after dyeing and simplifies theprocess of pollen activity identification of large quantities of materials, which is suitable for pollen activity identification of cotton, rice and other crops.

The invention provides an active oxygen quantitative detection kit. The kit is prepared from Nitro blue tetrazolium chloride monohydrate freeze-dried powder, a blue tetrazolium chloride monohydrate solution, a color developing agent A, a color developing agent B and a standard substance, wherein the chlorinated nitro tetrazole blue freeze-dried powder is powder obtained by freeze-drying blue tetrazolium chloride monohydrate liquid with a concentration of 0.1-1mmol/L; the blue tetrazolium chloride monohydrate solution is a phosphate buffer solution which has a concentration of 0.01-0.5mol/L anda pH value of 7.2-7.6; the color developing agent A is a weak acid buffer solution with a concentration of 0.01-0.3mol/L; the color developing agent B is 0.01-2% triethanolamine solution; the standard substance contains a hydrogen peroxide solution with a known concentration of 0-36mmol/L. The kit provided by the invention has the advantages that the method is scientific, clean and environmentally-friendly, manual operation and automatic batch inspection can be realized, and the kit is simple to operate and easy in clinical promotion.

The invention relates to a method for determining vitality of pseudo-ginseng pollen. The method comprises the following steps: using a cane sugar solution and a 2,3,5-triphenyl tetrazolium chloride solution to prepare a dyeing reaction solution; dropping the dyeing reaction solution on a cover glass, selecting the pseudo-ginseng pollen in the dyeing reaction solution on the cover glass, overturning the cover glass and placing the cover glass on a concave slide glass; placing the pollen in a thermotank for culturing; taking the pollen out and placing the pollen under a microscope for observation, selecting the visual field at random, and counting the proportion of dyed pollen grain number and the undyed pollen grain number, and wherein the pseudo-ginseng pollen with 30% or more has vitality. The method has the beneficial effects of simple operation, convenient and practical performances, high accuracy, and reliable statistics data, and has high practical application value, and has active promotion effect for breeding efficiency, output and quality of pseudo-ginseng.

The invention provides a method for quantitatively determining viability of barley seeds, belongs to the technical field of evaluation of the viability of the barley seeds, and can evaluate germination potential of the barley seeds rapidly and stably and provide reference for seed purchase and production. According to the method, TTC (triphenyl tetrazolium chloride) is used for dyeing dissected barley seeds, the dyed barley seeds are crushed with a freeze-drying and ball-milling crushing combined technique, a 10% trichloroacetic acid methanol solution is adopted as extract liquor to perform extraction on the crushed barley seeds, TTF (1,3,5-triphenylformazan) is obtained, and content of TTF is analyzed quantitatively according to a TTF standard curve with absorption photometry to predict the viability of the barley seeds. With the adoption of the freeze-drying and ball-milling crushing combined technique, loss of a sample in an operation process is reduced, and an experimental result is more precise and more reliable. By separating and dissecting the barley seeds, interference of useless substances in follow-up experiments is reduced, reaction volume is reduced, and experiment costand workload are saved.

The invention relates to the technical field of analytic chemistry detection, in particular to a quick detection method of hydrocortisone. The quick detection method includes: taking a to-be-detected sample, adding a hydrochloric solution, shaking, adding ethyl acetate, shaking, and centrifuging or standing for layering; taking centrifugate or upper-layer solution, adding a sodium hydroxide solution, shaking, and centrifuging or standing for layering; dripping the sodium hydroxide solution onto a white drip plate, dropwise adding a triphenyl tetrazolium chloride test solution, mixing well, dropwise adding a test sample solution, and observing color change. The quick detection method is simple and convenient to operate and low in analysis cost; interference of related factors is eliminated, and observation of solution color is facilitated better; extracting liquid, washing liquid, leaching liquid and a detection agent which are used in the method are high in specificity; interference substances are separated and removed by utilizing dissolubility difference of different substances for three times in the process of extracting, washing and leaching, so that sensitivity of chromogenic reaction is improved, detection result accuracy is high, the chromogenic reaction is quick and obvious in phenomenon, and the quick detection method is suitable for on-site quick detection.

The invention relates to the technical field of analytical chemical detection, in particular to a quick detection method of dexamethasone; comprising the steps of taking a sample to be detected adding hydrochloric acid solution, shaking, adding ethyl acetate, shaking, and centrifuging or standing to allow layering; taking supernate or upper solution, adding sodium hydroxide solution, shaking, and centrifuging or standing to allow layering; dropping sodium hydroxide solution to a white dropper plate, dropwise adding triphenyl tetrazolium chloride test solution, mixing well, dropwise adding test sample solution, and observing changes in color. The method of the invention is simple to perform and low in analytical cost; despite of the influence of related factors, the observation of solution color is more facilitated, the abstracting liquid, washing liquid, extracting liquid and detection agents used herein are highly specific, interfering substances are separated and removed by using dissolution differences of different substances three times in the abstracting, washing and extracting processes, the sensitivity of color reaction is improved, detection results accuracy is high, the color reaction is quick, phenomena are significant, and the method is suitable for quick field detection.

The invention relates to phenyltetrazolium chloride, and in particular, relates to a preparation method of phenyltetrazolium chloride. The preparation method comprises the steps: with benzaldehyde, aniline and phenylhydrazine as basic raw materials, synthesizing phenylhydrazone by benzaldehyde and phenylhydrazine firstly, then synthesizing triphenylformazan red crystals from diazonium salt formedby aniline, and then cyclizing into 2,3,5-triphenyltetrazolium chloride by using an organic catalyst. The process completely avoids use of lead tetraacetate as the catalyst to synthesize the product.Compared with a traditional process, the preparation method does not use lead tetraacetate, eliminates the influence of lead on the environment, and has the yield reaching 70% or more and the purity of more than or equal to 99% (HPLC).

The invention discloses a method for screening oil-producing filamentous fungi, and particularly relates to a triphenyl tetrazolium chloride PDA culture medium suitable for the oil-producing filamentous fungi. The culture medium is prepared by the following steps: preparing a potato agar culture medium; and adding triclosan and triphenyl tetrazolium chloride, so that the potato agar culture mediumcontains 5 mg/L triclosan and 0.5 g/L triphenyl tetrazolium chloride. The method for screening the oil-producing filamentous fungi comprises the following steps: taking soil in sterile water, performing oscillating for 1-3 hours, and diluting a bacterial liquid; and coating the triphenyl tetrazolium chloride PDA culture medium with the bacterial liquid, separating oil-producing filamentous fungiand/or oil-producing filamentous fungi spores, and performing purifying to obtain thalli. According to the invention, the culture medium is preferably prepared, the oil-producing filamentous fungi arescreened, and lipid extraction is carried out on the oil-producing filamentous fungi, so that the oil obtaining efficiency is improved.

The invention discloses a low-temperature dry preservation method for paphiopedilum pollen. The method comprises the following steps that within 3-5 days when a paphiopedilum flower is completely unfolded, an anther cap of the flower is opened by using a sterilized picking tool; a paphiopedilum pollen mass is picked to a clean and sterile container by using the sterilized picking tool, and the pollen mass is dehydrated under a sterile state; the dehydrated pollen mass is well sealed, placed in an environment with the temperature of 4 DEG C and preserved in a dark place. The preservation time of the paphiopedilum pollen mass preserved by using the method can reach 6-12 months, and after the pollen mass is subjected to re-warming and rewetting, by determining the pollen viability through a triphenyl tetrazolium chloride staining method, it is found that the viability of the pollen preserved by using the method is high, and the selfing or hybridization success rate is up to 90-95%. By means of the method, the barriers of cross pollination of types and plants of paphiopedilum in different blooming periods are overcome, a foundation is laid for the further development of the culture and industries of new varieties of Chinese paphiopedilum, and therefore, the method has great social benefits and economic benefits.

A method of detecting microorganisms in blood is provided. The method includes (1) a step of directly adding 2,3,5-triphenyltetrazolium chloride (TTC) into a blood culture flask, or a step of mixing 2,3,5-triphenyltetrazolium chloride into a silicone rubber oxygen permeable membrane to obtain a solid indicator and fixing the solid indicator to the bottom of the blood culture flask, with the concentration of the TTC being 0.01-0.03 g/mL, (2) a step of adding a blood medium into the blood culture flask, and sterilizing at 121 DEG C for 20 min, (3) a step of adding a blood sample into the blood culture flask, and culturing at 35 DEG C for 0-5 days, and (4) a step of detecting intensity of reflected light of an index strip in the blood culture flask, mapping an intensity curve of the reflected light, and determining a microorganism growth result according to the curve. The method is advantaged by low costs, simple operation processes, short detection time, high positive detection rates, and the like in clinical application, and is high in application value.