Method of quickly identifying bacterial wilt resistance in tobacco varieties in seedling stage

A bacterial wilt and tobacco technology, applied in the identification field of bacterial wilt resistance of tobacco varieties, can solve problems such as difficulty in large-scale popularization and application, long time required for identification, and large influence of environmental factors, etc., to achieve good infection effect, The identification results are accurate and reliable, with good repeatability and consistency

Pending Publication Date: 2017-09-29
四川省农业科学院经济作物育种栽培研究所 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural infection identification has the advantage of easy operation, but it is greatly affected by environmental factors, the incidence is uneven, and the identification takes a long time; artificial inoculation identification is characterized by its standard method, rapid onset, short identification time, and accurate identification results. Widely used by researchers and plant protection workers
[0005] However, in the existing identification method of artificial inoculation of tobacc...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0029] A method for rapidly identifying bacterial wilt resistance of tobacco varieties at the seedling stage, the steps of which are:

[0030] A. Cultivation of test tobacco seedlings:

[0031] The tobacco seedlings of the tested tobacco varieties were cultivated in the nutrient pot according to the method of floating seedlings, and no fungicide was used in the process of seedling cultivation;

[0032] B, preparation of bacterial wilt inoculum:

[0033] Tobacco bacterial wilt disease strains collected from the field are isolated and purified by TTC medium, that is, cultured on TTC medium for 3 days at a temperature of 25° C. to obtain purified and cultivated R. solanacearum; the TTC The medium is prepared by 12 parts by weight of peptone, 0.8 parts by weight of hydrolyzed milk protein, 13 parts by weight of glucose, 20 parts by weight of agar, 55 parts by weight of triphenyltetrazolium chloride, and 1000 parts by weight of distilled water ;

[0034] Inoculate R. solanacearu...

Embodiment 2

[0044] A method for rapidly identifying bacterial wilt resistance of tobacco varieties at the seedling stage, the steps of which are:

[0045] A. Cultivation of test tobacco seedlings:

[0046] The tobacco seedlings of the tested tobacco varieties were cultivated in the nutrient pot according to the method of floating seedlings, and no fungicide was used in the process of seedling cultivation;

[0047] B, preparation of bacterial wilt inoculum:

[0048] Tobacco bacterial wilt disease strains collected from the field were isolated and purified by TTC medium, that is, cultured for 5 days at a temperature of 28° C. to obtain purified and cultivated R. solanacearum; the TTC medium was composed of 8 Prepared by weight of peptone, 1.2 parts of hydrolyzed milk protein, 7 parts of glucose, 15 parts of agar, 45 parts of triphenyl tetrazolium chloride, and 1000 parts of distilled water;

[0049] Inoculate R. solanacearum purified and cultivated in TTC medium on NA medium, and culture ...

Embodiment 3

[0059] A method for rapidly identifying bacterial wilt resistance of tobacco varieties at the seedling stage, the steps of which are:

[0060] A. Cultivation of test tobacco seedlings:

[0061] The tobacco seedlings of the tested tobacco varieties were cultivated in the nutrient pot according to the method of floating seedlings, and no fungicide was used in the process of seedling cultivation;

[0062] B, preparation of bacterial wilt inoculum:

[0063] Tobacco bacterial wilt disease strains collected from the field were isolated and purified by TTC medium, that is, cultured with TTC medium for 4 days at a temperature of 27° C. to obtain purified and cultivated R. solanacearum; the TTC The medium is prepared by 10 parts by weight of peptone, 1.0 parts by weight of hydrolyzed milk protein, 10 parts by weight of glucose, 18 parts by weight of agar, 50 parts by weight of triphenyltetrazolium chloride, and 1000 parts by weight of distilled water ;

[0064] Inoculate R. solanace...

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Abstract

A method of quickly identifying bacterial wilt resistance in tobacco varieties in seedling stage comprises the main steps of A, raising seedlings of a tobacco variety under test in nutrient pots according to a floating seedling raising method; B, subjecting tobacco plants infected by bacterial wilt and collected from a field to isolation purification culture for 3-5 days via a TTC (triphenyl tetrazolium chloride) medium to obtain purified Ralstonia solanacearum; inoculating the Ralstonia solanacearum subjected to purification culture to NA (nutrient agar) medium, and culturing for 12 h to obtain an inoculation bacterial liquid; C, sucking the inoculation bacterial liquid with an injector, selecting tobacco seedlings growing with 3-4 true leaves in the nutrient pots in step A, and injecting 2-4 mu L of the inoculation bacterial liquid obliquely into a stalk of each selected tobacco seedling from its axil; D, culturing the inoculated tobacco seedlings in a sunlight culture room for 10 days, observing and recording disease attack conditions of the tobacco seedlings on 10th day of culture, calculating a disease index, and judging the bacterial wilt resistance of the tobacco variety under test. The method is simple to perform, high in measuring speed, good in results accuracy and good in result uniformity.

Description

technical field [0001] The invention relates to a method for identifying tobacco variety bacterial wilt resistance. Background technique [0002] Tobacco is an important economic crop in my country and even in the world. Tobacco bacterial wilt caused by Ralstonia solanacearum (Ralstonia solanacearum) is one of the main diseases of tobacco, and the annual yield loss caused by it is about 20%, and the severe one can reach 50%, or even no harvest, which seriously affects the yield and quality of tobacco leaves. quality. The infection sources of tobacco bacterial wilt mainly include soil with bacteria, diseased residues, and pathogenic bacteria invade from tobacco wounds. In recent years, due to continuous cropping of tobacco, the incidence and area of ​​soil-borne diseases such as bacterial wilt have increased year by year. At present, chemical control methods are mainly used to control bacterial wilt in production. However, the long-term and large-scale application of chem...

Claims

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Application Information

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IPC IPC(8): A01G31/00
CPCA01G31/00
Inventor 曾华兰华丽霞叶鹏盛何炼蒋秋平韦树谷罗定棋徐传涛代顺冬余祥文李斌李琼英张骞方张敏黄玲
Owner 四川省农业科学院经济作物育种栽培研究所
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