79 results about "Triphenyltetrazolium chloride" patented technology

The invention relates to a kit for detecting food-borne pathogenic bacteria, which comprises 12 groups of primers and probes thereof, wherein in the primers, the 5' ends of downstream primers are all tagged with biotin. The kit amplifies nucleic acid in a sample to be detected by utilizing the primers, and a formed biotinylated product and a probe fixed on the surface of a chip are hybridized to form a probe-biotinylated product compound; an alkaline phosphatase-streptavidin conjugate is combined with the probe-biotinylated product compound, and then chromogenic substrate which is tetranitroblue tetrazolium chloride/5-bromine-4-chlorine-3-indolyl-phosphate (NBT/BCIP) is reacted with alkaline phosphatase to undergo a colour generation (bluish violet) reaction; and whether the sample to be detected contains the pathogenic bacteria to be detected is detected by interpreting whether a specific site has colour generation. The kit has the advantages of high detection throughput, high speed, high sensitivity, high specificity and great practicability. The invention also provides the method for detecting the food-borne pathogenic bacteria by adopting the detection kit.

The invention belongs to the field of medical dressings, and particularly relates to color-developing medical dressing and a preparation method thereof. The preparation method comprises the following steps: taking TTC (Triphenyl Tetrazolium Chloride) as a color developing agent, mesoporous silica nanoparticles as a carrier and alginate and sodium carboxymethyl cellulose as spinning raw materials, preparing fiber containing color developing components through electrostatic spinning, and then preparing the fiber into the medical dressing containing the color developing components through a non-woven fabric technology. The color-developing medical dressing prepared by the preparation method has very good biological safety, and has an indicating function on a bacteria-infected wound; meanwhile, the color-developing medical dressing has very good liquid absorptivity, and is capable of absorbing leakage liquid of the wound and promoting the healing of the wound.

A method of quickly identifying bacterial wilt resistance in tobacco varieties in seedling stage comprises the main steps of A, raising seedlings of a tobacco variety under test in nutrient pots according to a floating seedling raising method; B, subjecting tobacco plants infected by bacterial wilt and collected from a field to isolation purification culture for 3-5 days via a TTC (triphenyl tetrazolium chloride) medium to obtain purified Ralstonia solanacearum; inoculating the Ralstonia solanacearum subjected to purification culture to NA (nutrient agar) medium, and culturing for 12 h to obtain an inoculation bacterial liquid; C, sucking the inoculation bacterial liquid with an injector, selecting tobacco seedlings growing with 3-4 true leaves in the nutrient pots in step A, and injecting 2-4 mu L of the inoculation bacterial liquid obliquely into a stalk of each selected tobacco seedling from its axil; D, culturing the inoculated tobacco seedlings in a sunlight culture room for 10 days, observing and recording disease attack conditions of the tobacco seedlings on 10th day of culture, calculating a disease index, and judging the bacterial wilt resistance of the tobacco variety under test. The method is simple to perform, high in measuring speed, good in results accuracy and good in result uniformity.

The invention discloses a method for tissue culture fast propagation of bletilla. The method specifically comprises the following steps of sterilization of an explant, sterile germination of bletilla seeds, observation of seed embryo form and seed coat breaking process, tissue culture fast propagation of bletilla, and determining of bletilla seed activity by a TTC (triphenyltetrazolium chloride) method. The method has the advantages that the method of obtaining bletilla seedlings is simple, and the seedling culture cost is greatly reduced; the germination is quick, the proliferation rate is high, the method is simple, the adding of tissue culture equipment is not needed, the production cost is low, the batched production is realized, and the application value is high.

The invention relates to screening and application of yeast CGMCC 4747 for high production of ethanol and low production of fusel oil in production of Chinese Maotai-flavor liquor, belonging to the technical field of biological engineering. A strain is of saccharomyces cerevisiae CGMCC 4747. The strain is obtained by separation from various Daqu, fermented grains, brewing raw materials and brewing environments of multiple famous liquor factories in China; and the screening method comprises the following steps of: taking substances of Daqu samples, fermented grains, brewing raw materials or brewing environments, diluting, coating a TTC (triphenyltetrazolium chloride) prescreening flat plate, selecting colonies with significant red color, further transferring into shaking flask culture for rescreening, determining the content of each of the ethanol and the fusel oil in a fermentation solution by adopting a distillation specific gravity method and HS-SPME-GC-MS respectively, and screening to obtain the strain with high concentration of the ethanol and low concentration of the fusel oil. The strain can be applied to food industry and brewed liquor industry.

The invention discloses a quantitative high-vitality yeast cell screening method based on a TTC staining method. The quantitative high-vitality yeast cell screening method comprises the steps as follows: coating a plate with a yeast cell liquid to be detected, and culturing the yeast cell liquid until single colonies grow; then inoculating the yeast cell liquid in a culture solution in each hole of a 96-hole cell culture plate, culturing the yeast cell liquid until OD values of thalli obtained through measurement are in a range of 1-3, centrifuging the liquid and abandoning supernatant; adding a TTF extracting agent to each hole of the 96-hole cell culture plate, and oscillating the liquid for 10-15S before centrifugation; and absorbing oscillated and centrifuged supernatant in a 96-hole EKISA plate, measuring a TTF absorption value under the wavelength of 486nm through an EKLSA reader, and calculating the content of TTF in each hole according to a TTF standard curve and the measured cell OD values to determine the vitality of cells. Screening results reflect the vitality of the cells directly and reliably, the screening time can be reduced obviously, and human errors caused by visual judgments during an operation process can also be reduced.

The invention discloses a detection method for the growth state of the roots of banana temporarily-planted seedlings, comprising the following steps of: (1) selecting banana temporarily-planted seedlings transplanted into a seedling culture device from a seedbed and then culturing for 7-35 days, cutting off pseudostem parts, and performing cutting treatment in the seedling culture device to obtain the roots of the banana temporarily-planted seedlings; (2) placing the roots of the banana temporarily-planted seedlings in reaction solution containing 2,3,5-triphenyltetrazolium chloride, adjusting the temperature of a reaction to be 37+/-2 DEG C, and culturing for 2-3 hours in a dark environment to perform colouring reaction; and (3) after the colouring reaction, placing the roots of the banana temporarily-planted seedlings in stopping solution to stop the colouring reaction, taking out the roots of the banana temporarily-planted seedlings, and detecting the colouring condition of each part of the roots of the plants, wherein the higher the ratio of the red roots to the colourless roots in the same area is, the better the growth state of the roots of the banana temporarily-planted seedlings is. By the method, the growth state of the roots of the banana temporarily-planted seedlings can be rapidly and simply detected, a plurality of middle test steps can be reduced, error can be decreased, and operation procedures can be simplified.

The invention discloses a chromogenic medium and a quick test slip of streptococcus faecium. The chromogenic medium of the streptococcus faecium comprises peptone, yeast powder, sodium chloride, lactose, maltose, TTC (Triphenyltetrazolium Chloride), a bacteriostatic agent and an indicator. The quick test slip is a stable microbial culture device comprising a base plate, a water absorbing gel layer, a culture medium layer and a covering film; the chromogenic medium is absorbed on the culture medium layer. The chromogenic medium of the streptococcus faecium is accurate in testing results, obvious in display of positive results, high in specificity, convenient to observe and capable of performing qualitative detection and quantitative analysis to the streptococcus faecium at the same time. According to the quick test slip of the streptococcus faecium, the conventional multi-step detection and identification process is simplified to be done in one step; and in addition, the test slip is small in size and convenient to carry, can sample on site at any time, is simple and convenient to operate, and provides scientific basis and guidance for the water quality safety.

The invention discloses a test method for rapidly screening antibacterial residue in animal food samples, which comprises the steps of: strain activation, bacterial suspension preparation, test tube preparation, sample preprocessing and sample testing. The test method utilizes the principle that antibacterial can inhibit the growth or reproduction of bacteria sensitive to the antibacterial, a sample to be tested is sucked and added into a test tube, and is cultured for a certain period of time, and according to the change of the color of the culture medium in the test tube, whether the sample contains antibacterial residue or not is judged. Compared with the prior art, the test method keeps the reliability of the TTC (triphenyltetrazolium chloride) test method, and solves the problem that in the TTC test method, the interference of autochthonous microorganisms or miscellaneous bacteria in the food sample leads to false positive and false negative results; and the test method has the characteristics of simplicity, little interference, high sensitivity, high accuracy and the like, and is applicable to the high-throughput rapid screening of antibacterial residue in the animal food samples.

The invention discloses a method for quickly and qualitatively identifying new and old sunflower seeds based on a TTC (triphenyltetrazolium chloride) dyeing process. The method comprises the following steps: shelling unknown sunflower seeds according to different activities of sunflower seeds, then removing part of tails, dyeing seed kenels by adopting a chemical method which is the TTC dyeing method and finally qualitatively identifying new and old sunflower seeds to be detected according to dyeing ratio. The method is high in identification accuracy rate; the identification rate of new and old sunflower seeds is greater than 95%; the method is simple in the identification process; the method is reliable and effective for qualitatively identifying the new and old sunflower seeds.

The invention relates to screening and application of yeast CGMCC (china general microbiological culture collection center) 4748 with high ethanol yield and low fusel oil yield in production of Chinese Maotai-flavor liquor, and belongs to the technical field of biological engineering. The yeast strain is Saccharomyces exiguous CGMCC4748 and is obtained by separating in various yeasts for making hard liquor, fermented grains, fermenting materials and fermenting environments from many Chinese manufacturers of famous liquors. A screening method of the yeast strain includes thinning the yeasts, the fermented grains, the fermenting materials or fermenting environmental materials, coating to a TTC (triphenyltetrazolium chloride) primary-screening flat plate, selecting remarkably red colonies, and transferring the colonies to shaking culture for secondary screening, measuring content of ethanol and fusel oil in fermentation broth by distilling and proportioning process and HS-SPME-GC-MS (head space solid-phase micro-extraction and gas chromatography-mass spectrometry), and screening to obtain the strain with high ethanol concentration and low fusel oil concentration. The strain is applicable to food industry and liquor-making industry.

The invention discloses a method for measuring the waterlogging grade of Brassica oleracea L.var.botrytis L. crop and particularly provides a determination method for exploring the waterlogging grade of Brassica oleracea L.var.botrytis L. The triphenyltetrazolium chloride (TTC) reduction method is adopted to measure the root activity of Brassica oleracea L.var.botrytis L., Li-6400 is adopted to measure the photosynthetic parameters of Brassica oleracea L.var.botrytis L. leaves, an FMS-2 type portable pulse-modulated fluorescence instrument is adopted to measure the fluorescence parameters of Brassica oleracea L.var.botrytis L. leaves, the photosynthetic and physiologic indexes of Brassica oleracea L.var.botrytis L. are synthesized, and the parameters are optimized to determine the waterlogging grade of Brassica oleracea L.var.botrytis L. The method can quickly and accurately measure the maximal net photosynthetic rate, the maximal photochemical efficiency and the root activity, is high in operation feasibility, relatively high in measurement precision and relatively low in relative error, and can realize accurate calculation of the waterlogging stress index and waterlogging grade judgment of Brassica oleracea L.var.botrytis L.

The invention relates to a method for quickly screening filamentous fungi for producing polyunsaturated fatty acid. The method is characterized in that a fatty acid synthase inhibitor cerulenin is used as a resistance screening factor, triphenyltetrazolium chloride characterizing the activity of dehydrogenase is used as a screening indicator, and a method for quickly and efficiently screening the filamentous fungi for producing the polyunsaturated fatty acid at high yield from environment is established. The method disclosed by the invention is simple and quick in process; the high-yield filamentous fungi is efficiently screened, and great convenience is provided for screening and researching filamentous fungi for producing oil; meanwhile, the invention provides a high-efficiency screening method and a batch of effective original strains for producing the polyunsaturated fatty acid by utilizing microbial fermentation.

The present invention relates to application of a live cell sensor in cryopreservation of plant cells. For the first time, the live cell sensor is used as a method to quickly evaluate the cryopreservation effect on the cells, and the cryopreservation effect of a cryoprotectant on the cells can be evaluated by changes in capacitance values of the living cells before and after cell freezing and resuscitation. By use of a living cell electrode, the concentration of the living cells can be quickly and accurately characterized. By combining of the capacitance values of the living cells with detection results of 2,3,4-triphenyltetrazolium chloride (TTC), the cryopreservation effect of a cryopreservation method on the cells can be evaluated rapidly and quantitatively, and inefficiency and no quantification of the preservation methods of the plant cells in the prior art can be overcome. Compared with the traditional methods, the comprehensive score of the cryopreservation of the cells can be improved by 5 times or more.

The invention discloses a method for quickly and efficiently screening an L-arginine-producing strain, belonging to the technical field of industrial microbial breeding. The traditional method for screening an L-arginine-producing strain is characterized by screening a strain containing anti-L-arginine structural analogs. In the invention, an Escherichia coli L-arginine defective strain is constructed as an L-arginine secretion indicator bacterium; and TTC (2,3,5-triphenyltetrazolium chloride) is a viable cell indicator and can be reduced into red by reducing hydrogen produced by viable cells. By using the L-arginine defective strain as the L-arginine secretion indicator bacterium and using the TTC as the developer through an interactive co-culture method to realize the qualitative screening of the L-arginine-producing strain based on the concentration of the extracellular L-arginine, a strain having high L-arginine synthesis capability or good secretion performance can be preliminarily screened on a flat plate. The method greatly improves the screening efficiency in strain breeding and saves the cost.

The invention discloses a method for preparing triphenyltetrazolium chloride. The method comprises the following steps: (1) synthesizing benzaldehyde; (2) synthesizing a diazonium salt; (3) synthesizing triphenyltetrazolium chloride. The method for preparing the triphenyltetrazolium chloride has the advantages that the process is simple, safe and easy to operate, and a catalyst used in the reaction process can be recycled, and thus the production cost is low; the synthesis method is environment-friendly, and is suitable for industrial production, and the wastewater discharge is greatly reduced; meanwhile, the product synthesized by the method has a yield of up to 88%.

The invention relates to a determination method of activity of stem tip cells of a plant. The method comprises the step of contacting a stem tip of the plant with a TTC (triphenyltetrazolium chloride) solution with a concentration of 0.45-0.55wt%. The activity of the stem tip cells of the plant can be quickly judged by using the method. The method is simple in procedure, easy to operate and high in efficiency and is an efficient path for quick determination of germplasm activity, especially the activity after ultralow temperature storage.

The invention relates to screening and application of yeast CGMCC 4740 for high production of ethanol and low production of fusel oil in production of Chinese Maotai-flavor liquor, belonging to the technical field of biological engineering. A strain is of abnormal Pichia pastoris CGMCC4740. The strain is obtained by separation from various Daqu, fermented grains, brewing raw materials and brewingenvironments of multiple famous liquor factories in China; and the screening method comprises the following steps of: taking substances of Daqu samples, fermented grains, brewing raw materials or brewing environments, diluting, coating a TTC (triphenyltetrazolium chloride) prescreening flat plate, selecting colonies with significant red color, further transferring into shaking flask culture for rescreening, determining the content of each of the ethanol and the fusel oil in a fermentation solution by adopting a distillation specific gravity method and HS-SPME-GC-MS respectively, and screeningto obtain the strain with high concentration of the ethanol and low concentration of the fusel oil. The strain can be applied to food industry and brewed liquor industry.

The invention relates to a method for quickly detecting the vigor of morchella esculenta L. hyphae. The method includes the steps that after a bacterial strain to be detected is cultivated in mother strain flat plate culture media, a bacterium block on a flat plate is cut to be transferred into liquid deep layer culture media for shake culture, and accordingly bacterium liquid is obtained; the bacterium liquid is collected into a centrifuge tube for centrifuging, liquid supernatant is removed, the hyphae are extracted, and the hyphae are washed by normal saline; the hyphae are weighed and added with water to be matched into the bacterium liquid to be detected; the bacterium liquid to be detected, a Tris-HCl buffer solution and a TTC (2,3,5-triphenyltetrazolium chloride) solution are transferred into the centrifuge tube for reacting; after reacting is completed, reacting liquid is placed on ice immediately, centrifuging is conducted at the temperature of 4 DEG C, a cell sediment is obtained, and an extraction agent is added into the centrifuged cell sediment so that extract liquor can be obtained; the extract liquor is centrifuged, the liquid supernatant is collected, and the absorbance value is detected at the wave length of 485 nm. Compared with a traditional method that the vigor of strains is detected according to hypha characteristics, the method has the advantages of being stable and reliable in detection result, good in reproducibility, high in accuracy, convenient to operate, high in speed and the like.

The invention provides a TTC (2,3,5-triphenyltetrazolium chloride) chromogenic culture medium for drug sensitivity tests. The TTC chromogenic culture medium comprises BHI (brain heart infusion) broth, acid-hydrolyzed protein, soluble starch and TTC. The invention further provides a drug sensitivity kit. The drug sensitivity kit comprises a kit part and a drug-sensitive plate part, wherein the kit part comprises the TTC chromogenic culture medium. The invention further provides a preparation method of the drug sensitivity kit. The preparation method comprises preparation of the drug-sensitive plate part and preparation of the TTC chromogenic culture medium, and the drug-sensitive plate part is provided by the invention. The invention further provides a drug sensitivity test method. The drug sensitivity test method comprises steps of diluting a bacterial suspension with the culture medium, adding the diluted bacterial suspension to drug-sensitive holes and performing culture; the culture medium is the TTC chromogenic culture medium. With the adoption of the TTC chromogenic culture medium, the drug-sensitivity kit, the drug sensitivity test method and the drug sensitivity kit prepared with the preparation method of the drug sensitivity kit, drug sensitive test results can be directly and easily observed, deviation cannot be caused easily, drug sensitive report time is greatly shortened to only 4-6 h.

The invention belongs to the field of application of plant physiological and biochemical technology, and specifically relates to a method for rapid quantification of pollen activity at normal temperature or high temperature, and application. The method comprises the steps of determining formulations, dyeing concentrations, optimum dyeing times and applicable ranges of pollen TTC staining solutionat a normal temperature and a high temperature, preparing a combined dyeing solution formula: 5.23g of 1 mol/L potassium dihydrogen phosphate, 14.03g of dipotassium hydrogen phosphate and potassium phosphate buffer with pH of 7; dissolving 8g of 2,3,5-triphenyltetrazolium chloride (TTC) in the obtained potassium phosphate buffer, and adjusting the volume to 1L with distilled water to a final concentration of 8g/L. The invention can be used for quantitative detection of pollen activity of large quantities of materials, omits the microscopic examination operation after dyeing and simplifies theprocess of pollen activity identification of large quantities of materials, which is suitable for pollen activity identification of cotton, rice and other crops.