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Method for quickly and qualitatively identifying new and old sunflower seeds based on TTC (triphenyltetrazolium chloride) dyeing process

A qualitative identification, sunflower seed technology, applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc. There are no problems such as identification methods for seeds, and the effects of high discrimination accuracy, simple method, and reliable qualitative determination are achieved.

Inactive Publication Date: 2014-07-23
CHACHA FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no corresponding accurate identification method for the existing sunflower seeds, mainly through the naked eye observation and sniffing methods of experienced technology and purchasing personnel, the inspection efficiency is low, and the human judgment is not accurate.

Method used

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  • Method for quickly and qualitatively identifying new and old sunflower seeds based on TTC (triphenyltetrazolium chloride) dyeing process
  • Method for quickly and qualitatively identifying new and old sunflower seeds based on TTC (triphenyltetrazolium chloride) dyeing process
  • Method for quickly and qualitatively identifying new and old sunflower seeds based on TTC (triphenyltetrazolium chloride) dyeing process

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Experimental program
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Effect test

Embodiment 1

[0030] Described a kind of method based on TTC staining fast qualitative identification sunflower seed old seed, comprises the following steps:

[0031] (1) Spread the paper on the bottom of the Petri dish;

[0032] (2) Shell the sunflower seeds, remove part of the tail, and get melon seeds;

[0033] (3) Use TTC solution to soak the melon seeds, after filtering, spread the melon seeds on the bottom of the petri dish;

[0034] (4) Use a dropper to draw about 10ml of TTC solution, and evenly drop it on the melon seeds;

[0035] (5) The temperature of the incubator needs to be preheated to 48°C in advance, and the culture dish is placed in the incubator at 45°C for 30 minutes.

[0036] (6) Take out and open the petri dish, and immediately observe its color development quickly;

[0037] (7) The melon seeds that are dyed red are the new sunflower seeds with vitality, and the melon seeds that are not dyed are the old sunflower seeds that have no vitality or less vitality.

[003...

Embodiment 2

[0042] Rapid qualitative identification of new sunflower seeds using chemical reagents (TTC staining method).

[0043] The instruments and tools used are as follows:

[0044] Constant temperature incubator, electronic balance (0.01g), volumetric flask, beaker (1000ml, 200ml), glass rod, brown

[0045] Colored reagent bottles, thermometer, petri dish, round white paper (the same size as the bottom of the petri dish).

[0046] The reagent solutions used were prepared as follows:

[0047] Phosphate buffer (pH6~7) configuration:

[0048] Weigh 5.45g of sodium dihydrogen phosphate and 3.79g of disodium hydrogen phosphate, add appropriate amount of distilled water to fully dissolve them, transfer them to a 1000ml volumetric flask to constant volume.

[0049] Configuration of TTC solution (2.0%):

[0050] Weigh 2g of triphenyltetrazolium chloride (TTC) sample, put it into a 100ml volumetric flask, add pre-prepared phosphate buffer solution (pH6~7) to make it up to the mark, fully...

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Abstract

The invention discloses a method for quickly and qualitatively identifying new and old sunflower seeds based on a TTC (triphenyltetrazolium chloride) dyeing process. The method comprises the following steps: shelling unknown sunflower seeds according to different activities of sunflower seeds, then removing part of tails, dyeing seed kenels by adopting a chemical method which is the TTC dyeing method and finally qualitatively identifying new and old sunflower seeds to be detected according to dyeing ratio. The method is high in identification accuracy rate; the identification rate of new and old sunflower seeds is greater than 95%; the method is simple in the identification process; the method is reliable and effective for qualitatively identifying the new and old sunflower seeds.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for rapidly and qualitatively identifying new sunflower seeds based on a TTC staining method. Background technique [0002] The inspection of old sunflower seeds has always been an insurmountable gap for food companies. The sunflower seed raw material is seasoned every year. In the second year, food companies hope to use new raw materials for production, but there are old raw materials on the market. Driven by huge profits, vendors may use old seeds as new raw materials, or mix them into new raw materials in a certain proportion. In the seed, it cannot be identified during the purchase, and it is impossible to judge whether it can be purchased or not until the production after purchase. This will have a certain impact on the quality of the product. Based on such a situation, it is urgent to find a way to solve the problem of identifying old raw materials or mixin...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 陈先保
Owner CHACHA FOOD CO LTD
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