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Method for measuring waterlogging grade of Brassica oleracea L.var.botrytis L. crop

A determination method and technology for cauliflower, which are applied in the preparation of test samples, measurement of color/spectral properties, fluorescence/phosphorescence, etc., to achieve the effect of satisfying accurate calculation, satisfying the judgment of stain damage level, and high operational feasibility

Inactive Publication Date: 2015-08-26
NANJING UNIV OF INFORMATION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are certain limitations in studying the influence of flooding on a certain index alone, but there are few previous studies on the comprehensive measurement method for determining the waterlogging index of cauliflower, and there is no suitable measurement method.

Method used

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  • Method for measuring waterlogging grade of Brassica oleracea L.var.botrytis L. crop
  • Method for measuring waterlogging grade of Brassica oleracea L.var.botrytis L. crop
  • Method for measuring waterlogging grade of Brassica oleracea L.var.botrytis L. crop

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Step 1: Cauliflower Flooding Test

[0042] Transplant cauliflower at the seedling stage with uniform size and 3-5 true leaves into plastic pots. During the test, the water flooding group was set to submerge the soil in the pot by 1cm, and water was added appropriately to keep the water level in the pot consistent during the test; the CK group was set to normal management, just keep the soil moist, and the ambient temperature during the test was 23°C / 17°C , the relative air humidity is 72%, and the photosynthetically active radiation is 800μmol·m -2 ·s -1 . Each treatment set up 3 pots to replicate.

[0043] The second step: equipment and medicine preparation

[0044] (1) Instruments and equipment

[0045] 10-1000μL, 1000-5000μL DRAGONLAB adjustable pipette gun, Shanghai Wandao Instrument Technology Co., Ltd.;

[0046] UV spectrophotometer, Shimadzu Corporation, Japan;

[0047] Li-6400 portable photosynthesis instrument, Beijing Ligaotai Technology Co., Ltd.;

[...

Embodiment 2

[0085]According to the method of Example 1, cauliflower was used to carry out the flooding test, the physiological indicators were measured according to the above steps, the waterlogging stress index was calculated, and the waterlogging index was used to determine the disaster level. The results are shown in Table 2. It can be seen from the table that the change of comprehensive growth index of cauliflower over time under flooding conditions is consistent with the change trend of single index. The longer the flooding time, the higher the waterlogging stress index and the higher the disaster level. On the third day of flooding, the waterlogging stress index of cauliflower was mild, and on the sixth day, the waterlogging stress index of cauliflower was Q=2.05, and the waterlogging level was medium Under severe stress, after the 9th day, all indicators of cauliflower dropped sharply, Q>4, reaching severe waterlogging stress, and after 12 days of flooding, the activity of cauliflow...

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Abstract

The invention discloses a method for measuring the waterlogging grade of Brassica oleracea L.var.botrytis L. crop and particularly provides a determination method for exploring the waterlogging grade of Brassica oleracea L.var.botrytis L. The triphenyltetrazolium chloride (TTC) reduction method is adopted to measure the root activity of Brassica oleracea L.var.botrytis L., Li-6400 is adopted to measure the photosynthetic parameters of Brassica oleracea L.var.botrytis L. leaves, an FMS-2 type portable pulse-modulated fluorescence instrument is adopted to measure the fluorescence parameters of Brassica oleracea L.var.botrytis L. leaves, the photosynthetic and physiologic indexes of Brassica oleracea L.var.botrytis L. are synthesized, and the parameters are optimized to determine the waterlogging grade of Brassica oleracea L.var.botrytis L. The method can quickly and accurately measure the maximal net photosynthetic rate, the maximal photochemical efficiency and the root activity, is high in operation feasibility, relatively high in measurement precision and relatively low in relative error, and can realize accurate calculation of the waterlogging stress index and waterlogging grade judgment of Brassica oleracea L.var.botrytis L.

Description

technical field [0001] The invention belongs to the technical field of crop water stress measurement, and in particular relates to a method for measuring waterlogging level of cauliflower crops. Background technique [0002] Water is one of the key factors affecting the growth and development of crops. Only under the condition of suitable water, plants can carry out normal life activities. Insufficient or too much water will affect the physiology and morphology of crops. Studies have shown that: when the soil moisture is too much, it will cause hypoxia in the rhizosphere of the plant, inhibit aerobic respiration, rot the root system, and even cause rotten roots and dead seedlings (Zhao Kefu, 2003), that is, waterlogging. Root activity decreases, the absorption of soil water and nutrients is limited, and root growth stops, affecting the normal growth and development of plants (Kozlowski, 1997). Under flooding stress, leaf stomata are closed, transpiration is limited, and CO ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/64G01N1/28
Inventor 杨再强侯奇奇肖芳
Owner NANJING UNIV OF INFORMATION SCI & TECH
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