63 results about "Brassica sp." patented technology

The invention discloses a brassica plant drought resistance negative regulation factor BnaA9.TZF5 and application of the brassica plant drought resistance negative regulation factor BnaA9.TZF5. The BnaA9.TZF5 is a transcription factor gene for coding CCCH type zinc finger protein in brassica napus, and a knockout plant obtained by performing targeted editing on the BnaA9.TZF5 through CRISPR / Cas9 shows that the drought resistance is remarkably enhanced along with phenotypic changes such as increase of leaf epidermis wax deposition and reduction of water loss rate; and an overexpression plant of the gene shows that the wax is reduced, the water loss is accelerated and the drought resistance is weakened, so that the BnaA9.TZF5 is a negative regulation factor for the drought resistance of the brassica plant. The BnaA9.TZF5 gene expression is knocked out to improve the drought resistance of brassica plants, the BnaA9.TZF5 gene can be used for molecular breeding of crops such as brassica napus, Chinese cabbage, cabbage and mustard, and a novel drought-resistant improved target gene is provided.

Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic / transgene junctions within SEQ ID NO: 2 or with genomic / transgene junctions as set forth in SEQ ID NO: 12 and / or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

The application discloses a molecular marker for distinguishing early and late flowering of Brassica oleracea and application thereof, wherein the nucleotide sequence of the molecular marker exists in the non-coding region of chromosome 4 of the genome of early flowering Brassica oleracea, and does not exist in the non-coding region of chromosome 4 of the genome of late flowering Brassica oleracea; based on the molecular marker, a primer pair Bf5 is further designed, DNA of the Brassica oleracea is amplified by using the primer pair, and early flowering varieties or materials of the Brassica oleracea can be screened according to the presence or absence of the target band. The molecular marker and the primer pair provided by the application can be used for rapidly detecting early flowering varieties and materials of the Brassica oleracea, time and resources are greatly saved, selection efficiency is greatly improved, and thus the breeding process is accelerated.

The invention discloses a chitosan modified turmeric-broccoli exosome hybrid nasal spray and application thereof, and belongs to the technical field of biological medicine and drug delivery. The nasal spray is formed by dispersing double plant exosome-lipidosome hybrids in a carrier for nasal cavities. The preparation method comprises the following steps: fusing curcumin carried by a turmeric exosome and sulforaphane carried by a broccoli bud exosome with a pH response liposome membrane containing 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine, and modifying by chitosan to form a three-layer structure; the preparation method comprises the steps of exosome extraction, ultrasonic drug loading, liposome hybridization, chitosan coating and preparation blending. The nasal spray is high in entrapment efficiency and uniform in particle size, the chitosan enhances nasal adhesion, inflammation targeted drug release is achieved through pH response, the brain targeting efficiency and stability are excellent, and the adaptability to children is good. The curcumin and sulforaphane are used for targeted therapy of children autism spectrum disorder neuroinflammation, anti-inflammation and anti-oxidation are achieved through synergism of curcumin and sulforaphane, the curative effect is remarkable, and a new scheme is provided for treatment of related diseases.

The invention provides compositions and methods relating to the elevation of glucoraphanin compared to standard Brassica oleracea varieties. The invention also relates to the production of hybrid varieties having desired glucosinolate contents. The invention further provides plants, plant parts, and seeds comprising such traits and comprising a Myb28 allele from Brassica villosa that is not genetically linked to an ELONG allele from Brassica villosa.

The invention discloses a primer group for identifying a broccoli nuclear male sterility gene and application of a molecular marker, and belongs to the technical field of biology. The primer group comprises a first primer pair and a second primer pair, each primer pair comprises a forward primer and a reverse primer, and the sequences of the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the second primer pair and the reverse primer of the second primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 4 in a sequence table. By applying the primer group and the molecular marker, the fertility of the broccoli can be efficiently and accurately identified, the problem that 50% of fertile plants need to be pulled out in seed production is solved, and the manpower and material resource cost is reduced.

The present invention relates to a plant material for use as medicament, wherein the plant material is derived from a plant of the Brassica, Spinacia, or Lactuca genus, genetically modified to express a biologically active recombinant protein and comprises the biologically active recombinant protein as an active ingredient. The invention also relates to a method for producing such a plant material, and to a pharmaceutical composition comprising such a plant material and an orally acceptable support.

The application belongs to the technical field of molecular breeding, and particularly relates to a SCAR marker for detecting a heterologous radish mitochondrial fragment in a Brassica oleracea Ogura cytoplasmic male sterile line. The application provides a SCAR marker for detecting a heterologous radish mitochondrial fragment in a Brassica oleracea Ogura cytoplasmic male sterile line, which comprises one or more or all of markers AC1-AC16; the nucleotide sequences of the AC1-AC16 are shown in SEQ ID NO: 1-16. The SCAR marker of the application has high specificity and good stability, and can accurately identify whether the corresponding heterologous radish mitochondrial fragment is contained in a to-be-tested Brassica oleracea cytoplasmic male sterile line and the relative content thereof, thereby being beneficial to early selection of the Brassica oleracea cytoplasmic male sterile line, improving breeding efficiency, and reducing breeding cost.

The invention belongs to the technical field of microorganisms, and discloses application of verticillium lecanii in promoting cabbage growth. The verticillium lecanii JMC-01 (the preservation number is CCTCC NO: M2018303) is successfully colonized in a cabbage plant through spray inoculation, the invention further proves that the endophytic colonization of the verticillium lecanii JMC-01 has an obvious improvement effect on agronomic characters such as the plant height and the root length of the cabbage, and the verticillium lecanii JMC-01 has a good application prospect in the aspects of promoting the growth of the cabbage plant and increasing the yield of the cabbage.

This disclosure provides methods and compositions for identifying Brassica plants that have a native deletion of the INDEHISCENT gene (BnIND-A) located on chromosome A of B. napus. Also provided are methods of improving one or more agronomic characteristics such as pod shatter and breeding methods for introducing a pod shatter tolerant phenotype in Brassica plants and / or their progeny.

1. The name of the design product: packing bag (Brassica oleracea garlic hawthorn drink). 2. The use of the design product: a packing bag of a product. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: front view. 5. The design product is a thin product, and other views are omitted.

The invention provides an RPA (recombinase polymerase amplification) primer group, a kit and a method for detecting the alternaria brassicae of broccoli, which are used for detecting the alternaria brassicae of broccoli. The primer group comprises specific primers F1 / R1, F1 / R1 and F2 / R1, and the sizes of theoretical amplification products of the primer group are 204bp, 187bp and 189bp. Specific target genes are screened out through comparative genomics, RPA primers are designed, and a constant-temperature (39 DEG C) and rapid (20 minutes) detection method is established. The primer provided by the invention has strong specificity, is only effective to the brassica oleracea var. Brassicae, and has no cross reaction with sibling species; the sensitivity is high, the detection lower limit is 0.08 ng / mu L, and the sensitivity is 100 times higher than that of conventional PCR. The actual sample verifies that the detection accuracy reaches 100%. The method is easy and convenient to operate, rapid in detection and suitable for field early diagnosis, plant quarantine and disease monitoring of the pathogenic bacteria.

The invention discloses a method for high-yield cultivation of oxheart cabbages by using mountain tea garden conditions, relates to the technical field of cabbage planting, and aims to solve the problems of low resource utilization rate of northern-Sichuan mountain special tea gardens, poor cultivation suitability of spring-stubble oxheart cabbages and insufficient comprehensive benefits in the prior art. By combining the growth characteristics that the white-leaf No.1 tea tree variety and the middle-yellow No.1 tea tree variety grow slowly and grow weakly (plants are relatively short) in the North Sichuan tea area relative to general green tea and the actual construction of the white-leaf No.1 tea garden and the middle-yellow No.1 tea garden, the high-yield cultivation system of the oxheart cabbage adaptive to the white-leaf No.1 tea garden and the middle-yellow No.1 tea garden is constructed, and multiple beneficial effects are achieved. According to the method, the growth rhythm of'spring harvesting and summer cultivation 'of tea trees is accurately matched, relay intercropping is conducted after spring tea is harvested and pruned, the influence on spring tea core output is completely avoided, and the tea garden land and the light energy utilization rate are greatly improved.

The present disclosure provides Brassica oleracea plants having curds or heads exhibiting increased resistance to downy mildew. Such plants may comprise novel introgressed genomic regions associated with disease resistance from Brassica oleracea MYCOCLP. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.

The present invention relates to a composition based on ethanolic extract of Brassica olearacea var. capitata leaves, anhydrous lanolin and castor oil, for the treatment of burns and their complications, such as infections. Emulsions made from ethanolic extract of Brassica olearacea var. capitata leaves, anhydrous lanolin and castor oil in semi-solid formulations such as creams and ointments, with 10% and 20% w / v of Brassica extract, can be used as healing agents, accelerating closure of the skin area damaged by up to 3rd degree burns, inducing the production of protective enzymes as well as the proliferation of cells and blood vessels, modulating the production of matrix components and stimulating the formation of strong scar tissue, while having no side effects due to their lack of toxicity.

The invention belongs to the technical field of brassica plant breeding, and relates to a reagent and method for overcoming self-incompatibility of Chinese cabbage and application. After the Chinese cabbages enter the flowering phase, an EGCG (epigallocatechin gallate) solution is sprayed on the stigmas of the Chinese cabbages, so that the self-incompatibility of the Chinese cabbages can be weakened to different degrees. Experimental results show that after the EGCG is sprayed, the number of seeds obtained from each pod of the self-incompatible Chinese cabbage is increased to 17.8 from original 0. The used reagent EGCG does not pollute the environment, especially the soil environment, has small damage to plants, and can be applied to seed reproduction work of the Chinese cabbage self-incompatible line.

The invention relates to the use of a lytic bacteriophage, stabilised in a carrier, of the family Myoviridae, Foxunavirus genus (deposit IDAC 261122-01), which acts as a biocontroller of Xanthomonas campestris pv. Campestris (Xcc), a phytopathogen responsible for the main disease affecting brassicas globally, angular leaf spot or black rot. In addition, the invention relates to the preparation of a bioproduct comprising the phage and an agronomically acceptable carrier, such as alginate and / or chitosan.

This invention provides a genetically engineered *Yersinia lipolytica* strain and its applications. The genetically engineered strain is a *Yersinia lipolytica* Po1f strain with the PEX10 gene knocked out, expressing the C16 / 18 elongase gene MaELO3 from *Morchella alpina*, the elongase gene AtKCS from *Arabidopsis thaliana*, and the elongase gene CraKCS from *Brassica oleracea*. The strain also expresses the elongase gene CgKCS from *Capsella*. Further expression of the desaturase gene MaD15D from *Morchella alpina*, and overexpression of the strain's own diacylglycerol transferase gene DGA1 and its own Δ9 desaturase gene OLE1, resulted in a nervonic acid yield of 111.6 mg / L in shake-flask fermentation. By adding 1% rapeseed oil exogenously, the nervonic acid yield reached 185.1 mg / L. The *Yersinia lipolytica* genetically engineered strain constructed in this invention is simple to operate, has stable and reliable performance, and can be applied to large-scale commercial production.

This invention discloses a recessive nuclear male sterility gene. BoaGMS1 Application in regulating male development in Chinese kale. This invention screens recessive nuclear male sterility mutants from Chinese kale breeding and isolates the Chinese kale male fertility gene using forward genetics. BoaGMS1 Further experiments confirmed that BoaGMS1 A gene mutation resulted in recessive nuclear male sterility in Chinese kale. Meanwhile, targeting... BoaGMS1 Cosegregating molecular markers developed for male-sterile lines can be used for identifying fertility alleles in plants, screening target plants in marker-assisted breeding, and determining seed purity. Furthermore, they can be used in the development of kale. BoaGMS1 Male-sterile lines can be applied to the breeding and production of new hybrid varieties of Chinese kale. Therefore, the male fertility gene for Chinese kale provided by this invention... BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS It plays an important role in the utilization of heterosis and hybrid seed production of Chinese kale and even Brassica oleracea vegetables.

System for the development of a pH-based biosensor using anthocyanins from red cabbage (Brassica oleracea) and chitosan nanoparticles for monitoring food quality, comprising: ◯ Glassware for collecting and processing red cabbage samples, using a system for extracting anthocyanins by: • Thoroughly wash red cabbage with tap water and distilled water; • Chop the washed cabbage into fine pieces of about 1-2 cm; • Soaking 2 kg of shredded cabbage in 100 ml of acidified ethanol solution (ethanol:water:acetic acid, 70:29:1 v / v / v) for 24 hours; • Filter the extract using a vacuum filter and concentrate it under reduced pressure; • Store the extract at 4°C in a dark bottle; ◯ a device for the synthesis of chitosan nanoparticles via ionic gelation, comprising: • Prepare a 0.1% (w / v) chitosan solution in 1% acetic acid, adjusted to pH 4.6; • Add 0.1% tripolyphosphate (TPP) solution dropwise while stirring; • Collecting the nanoparticles by centrifugation and washing with deionized water; ◯ a system for introducing anthocyanins into chitosan nanoparticles by mixing the anthocyanin extract with the chitosan solution prior to TPP addition; ◯ a solution casting system for the production of a pH-sensitive biofilm by mixing anthocyanin-loaded nanoparticles with poly(vinyl alcohol); ◯ analytical instruments, including an Alpha E ATR-FTIR (Bruker, Germany) and an FEI QUANTA 450 scanning electron microscope, for characterizing the nanoparticles and biofilm; ◯ Design of a well diffusion test to assess antibacterial activity against Staphylococcus aureus using Mueller-Hinton agar plates.

Provided herein are various aspects related to increasing the proportion of omega-3 fatty acid in seed oil produced by a plurality of Brassica plants, which have been modified to produce seed oil comprising at least one of EPA, DHA and DPA. It relates to a method to increase the proportion of long-chain omega-3 fatty acid in seed oil produced by a plurality of Brassica oilseed plants comprising growing the Brassica oilseed plants in the presence of an increased amount of nitrogen as compared to a standard amount of nitrogen, wherein the Brassica oilseed plants have been modified to produce seed oil with at least one of EPA, DHA and DPA.

Brassica oleracea vegetable powder or composition containing same for regulating metabolic syndrome The present invention relates to the use of Brassica oleracea vegetable powder for regulating metabolic syndrome or a metabolic syndrome-related disease, for reducing the cholesterol and triglyceride content in cells and achieving the effect of improving or preventing metabolic syndrome or a metabolic syndrome-related disease, by administering to an individual an effective amount of Brassica oleracea vegetable powder or a composition containing Brassica oleracea vegetable powder.

1. The name of the design product: ice cream packaging bag (large kale fruit). 2. The use of the design product: for product packaging. 3. The design points of the design product: the combination of patterns and colors. 4. The picture or photo that best indicates the design points: front view. 5. The design for which protection is sought includes color. 6. The design product is a flat product, and the left view, right view, top view, and bottom view are omitted. 7. Other circumstances that need to be explained: the white areas indicated by A in the front view and B in the rear view are transparent designs.

The invention relates to the technical field of agricultural mechanization, in particular to a brassica oleracea harvesting header control method based on low-loss pulling and flexible clamping, which comprises the following steps: S1, collecting diameter data, shape and spatial position data of brassica oleracea entering a harvesting header area; s2, calculating the roller spacing of the current cabbage group; s3, calculating and dynamically adjusting the roller speed through an adaptive algorithm; s4, calculating a proper clamping force; s5, the tensioning force of the elastic tensioning device is adjusted; s6, on the basis of the S2 and the S3, the matching of the roller speed and the roller distance is controlled; and S7, calculating and adjusting the optimal angle and speed of the cabbages entering the conveying channel. By dynamically adjusting the roller speed, the roller distance and the clamping force and combining cabbage space position data, the angle of the conveying channel and the root cutting time are accurately controlled, damage to cabbages in the harvesting process is effectively reduced, the harvesting precision and efficiency are improved, and the intelligence and adaptability of the harvesting header are enhanced.

The invention is in the field of Brassica carinata breeding (i.e. Ethiopian mustard breeding), specifically relating to Brassica carinata variety Nujet 350. The present invention relates to seeds, plants or parts thereof, cells, methods of making, and uses of this variety and its progeny.

A cytoplasmic male sterile Brassica rapa plant is provided having a growth ability equivalent to that of a Brassica rapa plant having a normal cytoplasm or a progeny of the cytoplasmic male sterile Brassica rapa plant. For example, it is possible to improve the deterioration of growth ability which has been observed in the conventional cytoplasmic male sterile B. rapa plants and provide a cytoplasmic male sterile Brassica rapa plant having an improved growth ability.

A product consisting of (a) 20-50% in weight of a coextract of Filipendula leaves and flowers (17.5-32.5%), Laurus leaves (17.5-32.5%), Brassica seeds (17.5-32.5%) and Withania roots (17.5-32.5%), (b) 49-80% in weight of a coextract of Cynara leaves (10-19%), Curcuma roots (29-55%) and Tanacetum flowers (29-55%); and (c) 0.6-1.2% in weight of an extract of Agave leaves. The extracts can be freeze-dried in water. Filipendula can be Filipendula ulmaria and / or Filipendula vulgaris. Laurus can be Laurus azorica and / or Laurus nobilis. Brassica can be Brassica rapa, Brassica nigra, Brassica oleracea, and / or Botrytis cymose. Withania can be Withania siniensis and / or Withania somnifera. Cynara can be Cynara cardunculus scolymus and / or Cynara flavescens. Curcuma can be Curcuma zedoaria and / or Curcuma longa. Tanacetum can be Tanacetum cinerariifolium, Tanacetum parthenium and / or Tanacetum vulgare. Agave can be Agave americana and / or Agave sisalana. A further aspect is a therapeutic adjuvant or composition comprising the product. The composition can comprise an anticancer e.g., chemotherapeutic. A further aspect is a method of treating cancer by administering the product. A further aspect is a method of assessing the product by measuring 14C activity, assessing the presence of miRNAs and exosomes, and assessing the functional resilience between different batches of the product.

The present invention provides formulations with specific pesticidal activity against insects of the order Homoptera, based on a characterized strain of Bacillus megaterium which produces spores, crystals, primary and secondary metabolites, bioactive substances and degradative enzymes, where the bacteria and / or its fermentation products, both intracellular and extracellular are active ingredients, with pesticidal activity both in vitro and when applied in any phenological state of the plant, both to foliage, fruit, stem, root, for various botanical groups such as Bulbs; brassicas; legumes; nightshades; cucurbits; cereals; citrus, berries, forest and leafy vegetables.

The invention belongs to the technical field of molecular breeding, and particularly relates to a molecular marker and a primer group for detecting phenotypes of dominant nuclear gene female sterility (dominant female sterility, DGFS, Dominant Genic Feather Sterility for short) of cabbage, and application of the molecular marker and the primer group in identification of dominant female sterility single plants of the cabbage. The invention provides a linked molecular marker for detecting dominant female sterility phenotypes of cabbages. The linked molecular marker comprises molecular markers G1 and G2, the nucleotide sequences of the G1 and the G2 are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The invention also provides a primer group for amplifying the molecular marker, the primer group comprises primers G1p and G2p, and the nucleotide sequences of the primer pair G1p and G2p are as shown in SEQ ID NO: 3-6. The primer group disclosed by the invention is strong in specificity and good in stability, and can accurately identify whether a single plant of a cabbage segregation population is in a female sterile phenotype or not, so that early breeding of dominant female sterile single plants of cabbages is realized, and the breeding efficiency is improved.

The invention discloses a cabbage harvester which comprises a basic bearing frame, a cabbage harvesting device, an upward conveying mechanism, a backward conveying mechanism, a vegetable collecting box moving mechanism and a crawler-type walking mechanism. A diamond sand line root cutting mechanism is arranged on the cabbage harvesting device; according to the further improvement, a flexible supporting input mechanism is arranged above the cabbage harvesting device; an upper chain wheel conveying device is arranged above the lower chain wheel conveying device; in the upper chain wheel longitudinal circulating conveying belt, the second driving chains which are opposite at intervals are connected through a plurality of elastic belts; a long strip is spirally wound on the outer wall of the roller and is fixedly connected to the outer wall of the roller; the long strip is a hard plastic pipe; the vegetable collecting box movement mechanism is provided with a damage prevention structure and an automatic frame unloading device. The harvesting efficiency is remarkably improved, the loss of the brassica oleracea after the brassica oleracea is pulled to the ground is remarkably reduced, the conveying efficiency is remarkably improved through flexible wrapping, dead leaves on the brassica oleracea can be basically removed in the conveying process, trouble and time are saved, and damage to the brassica oleracea when the brassica oleracea falls into a vegetable collecting box is remarkably reduced.