329 results about "Brassica sp." patented technology

The invention relates to crop plants comprising novel seed lipid compositions. Provided are both wild type and mutant nucleic acid molecules encoding Brassica fatty acyl-acyl carrier protein (ACP) thioesterase B proteins (FATB) and the proteins as such. Also provided are Brassica plants, tissue and seeds comprising at least three mutant fatB alleles in their genome, whereby the seed oil fatty acid composition or profile is significantly altered.

This disclosure concerns a plant of the genus, Brassica, or parts thereof, which comprise one or more traits selected from the group consisting of high oleic acid content, low linolenic acid content, increased herbicide resistance, restorer of cytoplasmic male sterility, and increased clubroot disease (Plasmodiophora brassicae) resistance, compared to a wild-type plant of the same species. This disclosure further relates to wild-type and mutant alleles of genes involved in these traits, molecular markers linked thereto, and methods of their use.

In one aspect, of the invention provides novel tetraploid Brassica plants having no more than two expressible FAD2 coding sequences, capable of producing canola quality oils. Other aspects of the invention provides new variants of the FAD2 enzyme, comprising BjFAD2-b and BjFAD2-a, as well as nucleic acid sequences encoding such peptides. Other aspects of the invention includes nucleic acid sequences upstream from the BjFAD2-b or BjFAD2-a ORFs. Other aspects of the invention include transgenic plants and plant parts. Vectors capable of transforming plant cells are provided, comprising the nucleic acids of the invention, including FAD2 coding sequences. Corresponding methods are provided for obtaining the transgenic plants of the invention. Methods are provided for using the plants of the invention, including selected plants and transgenic plants, to obtain plant products. Amplification primers for identifying the FAD2 coding sequences of the invention are provided, together with methods of obtaining plants using the FAD2 coding sequences of the invention as markers.

The invention relates to the field of vegetable planting, in particular to a planting method of brassica oleracea L.var.capitata L.. The planting method of the brassica oleracea L.var.capitata L. comprises the following steps of A, variety selection; B, seedling growing; C, land preparation and arrangement of moisture in the soil; D, fertilization; E, transplantation and field planting; F, field management; G, insect disease prevention; H, timely harvesting. According to the planting method disclosed by the invention, insect disease can be effectively prevented from happening by selecting a disease-resistant variety and adopting seed sterilization treatment and soil sterilization treatment; seedlings are cultured by using a nutrition dish, the transplantation with soil can be carried out when in transplantation and field planting, the seedling stage can be shortened, and the tidy growth can be facilitated; dedicated organic-inorganic mixed fertilizer of the disease-resistant variety integrates organic and inorganic fertilizers into a whole, the problems of the lack of organic fertilizer and difficult fertilization of the organic fertilizer can be solved, the matching ratio of nitrogen, phosphorus and potassium is coordinated, and the fertilization efficiency is obvious; composting is carried out by adding a decomposition agent in tobacco powder, and a composting and slaking process of the tobacco powder can be quickened; the whole-life requirement on nutrient of a plant can be met just by carrying out hole fertilization of fertilizer in one time, and the labor is saved.

In various aspects, the invention provides Brassica juncea plants, seeds, cells, nucleic acid sequences and oils. Edible oil derived from plants of the invention may have significantly higher oleic acid content than other B. juncea plants. In one embodiment, the B. juncea line MJ02-357-3 contains a mutant allele MJ02-313-1/BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a G to A substitution in the ORF at position 281 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Glycine-94 Aspartic acid mutation in the sequence of the predicted BjFAD2-a protein. In another embodiment, the B. juncea line MJ02-357-3 contains a mutant allele MJ02-357-3/BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a C to T substitution in the ORF at position 647 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Proline-216 Leucine mutation in the sequence of the predicted BjFAD2-a protein. As a result of these mutations, it can be predicted that the function of the BjFAD2-a proteins are negatively affected in Brassica juncea lines MJ02-313-1 and MJ357-3 as reflected in the increased levels of oleic acid in seed oil in comparison with the wild-type line J96D-4830. Seeds from MJ02-313-1 and MJ02-357-3 plants may for example yield an oil having oleic acid content of greater than 70% by weight.

A supplement composition for enhancement of methylation process is provided, which contains vitamin B₆ (as pyridoxine HCl), folic acid, vitamin B₁₂ (as cyanocobalamin), betaine HCl, and methylsulfonylmethane; and also contains S-adenosylmethionine. The supplement composition further includes silymarin (from milk thistle seed extract), N-acetyl L-cysteine, and cruciferious blend which includes broccoli (brassica oleracea var. talica), kale (brassica oleracea var. acephala), and radish (raphanus sativus). Further provided is a method of using the supplement composition for enhancement of methylation process.

The invention discloses a method for identifying Chinese cabbage-cabbage addition lines. The method comprises the following steps: (1) selecting 91 pairs of primers in different linkage groups of cabbage according to a published cabbage genetic map; (2) extracting genetic groups DNA of different Chinese cabbages and cabbages to carry out PCR amplification; (3) electrophoretically separating an amplified product in modified polyacrylamide gel of 6 percent to carry out silver staining; (4) screening SSR primers in the linkage groups of the cabbage different from the Chinese cabbage; and (5) identifying the Chinese cabbage-cabbage addition lines by the SSR primers in the linkage groups of the cabbage different from the Chinese cabbage. The method has the advantages that the method can quickly and accurately identify important genetic analysis materials for the Chinese cabbage and the cabbage, such as coenospecies, addition lines, alien substitution lines, translocation lines and the like on molecular level, and lays foundation for modifying Chinese cabbage genetic background by cabbage gene.

The invention discloses a method of improved artificially synthesized Brassica napa L. for heterosis utilization, belonging to the technical field of breeding plant novel varieties. The method comprises the following steps: transferring the excellent oil economical characters (the content of thioglycoside in seed cake dregs is lower than 30 mu mol/g, and the content of erucic acid in seeds is lower than 1% of double-low Brassica napa L.) of the double-low Brassica napa L. to wild cabbage; culturing a novel oil wild cabbage material, hybridizing the material with a double-low Brassica campestris variety to obtain the artificially synthesized Brassica napa L. with normal fecundity, wherein, the content of thioglycoside in seed cake dregs is lower than 30 mu mol/g, the content of erucic acidin seeds is lower than 1%, and the superior combination prepared by the artificially synthesized Brassica napa L. and the natural Brassica napa L. is screened. In the invention, the good genes of Brassica napa L. are transferred to wild cabbage to culture oil wild cabbage material which is for overcoming the disadvantages of low quality and low fecundity of the existing artificially synthesized Brassica napa L., thereby improving the potential of artificially synthesized Brassica napa L. for heterosis utilization.

The invention discloses a nitrilase N1 derived from pantoea sp.AS-PWVM4 and a gene thereof, a nitrilase N2 derived from arabidopsis thaliana and a gene thereof, a nitrilase N3 derived from acidovoraxfacilis 72W and a gene thereof, a nitrilase N4 derived from leptolyngbya sp. and a gene thereof, a nitrilase N5 derived from brassica oleracea var.oleracea and a gene thereof and a nitrilase N6 derived from camelina sativa and a gene thereof, and a method for preparing paracyanobenzoic acid as p-aminomethylbenzoic acid intermediate by using the nitrilase as a biological catalyst; resting cells ofthe corresponding nitrilases can be used for catalyzing 100g/L of substrate; the conversion rate is greater than 99%; the method has the obvious characteristics of mild reaction conditions, no pollution and simple process route, and has broad industrial application prospects.

The invention discloses a molecular marker and specific primers for assisting in test of wilt disease resistance in brassica oleracea and use thereof. The invention provides a reagent for assisting in the test of wilt disease resistance in brassica oleracea and/or assisting in screening brassica oleracea with wilt disease resistance, which is a specific primer pair formed by nucleotides represented by a sequence 2 and a sequence 3 in a sequence table. The invention also provides a specific gene fragment which is at a genetic distance about 2.87cM to the wilt disease resistance gene in brassica oleracea and is formed by nucleotides represented by a sequence 1 in a sequence table. The result of the identification of wilt disease resistance in brassica oleracea and/or screening of the brassica oleracea with wilt disease resistance with assistance from the reagent (primer pair) or sequence characterized amplified region (SCAR) marker, which are provided by the invention, is 97 percent consistent with that of field identification. When used in breeding, the reagent, molecular marker or method has the advantages of accuracy, quickness, capability of realizing early breeding and the like and has a bright application prospect.

The invention relates to the field of vegetable plantation, in particular to a method for planting brassica oleracea L.var .botrytis L..The method for planting brassica oleracea L.var .botrytis L.comprises the following steps of 1, variety selection; 2, seedling culture; 3, soil preparation and soil moisture regulation; 4, fertilizer application; 5, transplanting and field planting; 6, field management; 7, disease and insect pet prevention; 8, leaf binding and flower protection; 9, harvesting conducted in good time.The method is simple and convenient to operate, brassica oleracea L.var .botrytis L.can grow vigorously, resist to diseases and be high in quality and yield, cost can be saved, and synergism, ecological efficiency and environmental protection can be achieved.

The invention discloses a brassica oleracea seed production technology, an adopted seed production method comprises the steps that a lateral bud is cut from a knot position and cultured on a culture medium, and subculture is performed in a lab continuously to obtain an original mother plant; seed reproduction of a sterile line is performed; a sun bed is prepared; a seeding tray is prepared; provisonal planting of the mother plant is performed; maintainer line seedling growing is performed; field planting is performed; pollination is performed; ventilation harvesting is performed; in the podding mid-term, maintainer line rows are removed; the row space is increased to facilitate ventilation and light transmitting, 70% of pods are cut when yellowing and dried for threshing after ripening; male parent seed reproduction is performed; the bud selection standard is strictly required, the bud stripping and pollination operation is standardized, and pollination marks are made; coeno-species seed reproduction is performed. According to the seed production technology, the yield can reach 65-75 kg/mu, the germination rate and the germination potential can reach up to 85% or above, the seed yield is high, and a foundation is laid for brassica oleracea stable production; after planting, the species has remarkable advantages over the other species on whether the disease resistance, the cold resistance, the physical shape or the yield, and the brassica oleracea is convenient to popularize, promote and plant.

The present invention relates to an ALS inhibitor herbicide tolerant crop plants, such as allotetraploid Brassica plants, such as B. napus plants, progeny and parts thereof comprising mutations in acetolactase genes.

The invention discloses a method for introducing radish chromosomes d into cabbage. The method comprises the following steps of: performing sexual hybridization on a cabbage type rape-radish chromosome d addition line and cabbage; introducing the radish chromosomes d into the cabbage; selecting a cabbage plant containing the radish chromosomes d by adopting a molecular marker detection and cell microscopy method; and creating a new cabbage germplasm carrying the radish chromosomes d. In the method, cabbage type rape is taken as a carrier of the radish chromosome d, and is hybridized with cabbage to transfer the radish chromosome d to the cabbage, so that direct hybridization between the cabbage and radish is avoided. Hybridization in brassica alboglabra succeeds easier than intergeneric hybridization between brassica alboglabra and radish. Moreover, only one pair of radish chromosomes is integrated with a cabbage genome, so that more genetic drag caused by the hybridization of an entire radish genome can be avoided, and the transfer and selection efficiencies of target traits can be improved. By adopting the method, a novel thought is provided for distant hybridization utilization.

The invention relates to a method for obtaining collard regenerated plant and special cultivation base. Wherein, said method comprises (1), planting the collard into induced cultivation base, until the density is 5*10<4-5*10<5n/mL, at 30-35Deg.C and in dark, treating it at high temperature for 24-72hours, and under 24-26Deg. C and in dark, inducing idiosome; (2), when the idiosome has been formed, under 24-26Deg. C, 1500-2500Lux, cultivating for 5-10days while each day lights 14-18hours; then grafting the seed into differentiation cultivation base, to be cultivated in same conditions; (3) when grows out branch and leaf, transplanting it into rooting cultivation base, under 24-26Deg. C, 1500-2500Lux, lighting 14-18hours each day, to root, to obtain the collard regenerated plant.

The invention relates to fungal disease resistance, in particular to resistance to blackleg disease caused by Leptosphaeria maculans. Provided are Brassica plants and seeds comprising a fragment of chromosome 8 of a wild B. rapa accession in their genome, wherein this fragment comprises a blackleg resistance locus. Further provided are molecular markers linked to the blackleg resistance locus and methods of using the markers. Brassica plants and seeds with stacked blackleg resistance loci are also provided.

The invention relates to crop cultivation and in particular relates to a method for increasing content of anthocyanin in purple cabbage. The method for increasing the content of anthocyanin in the purple cabbage comprises the following steps: rinsing purple cabbage seeds with clear water, then soaking the purple cabbage seeds in melatonin solution for 12-24 hours at the temperature of 20-30 DEG C, washing out and then sowing. The invention provides a new method for promoting accumulation of anthocyanin in the purple cabbage, 10-1000mu mol/L melatonin solution is utilized for soaking the purple cabbage seeds, and anthocyanin content of purple cabbage seedlings can be obviously increased.

The invention discloses a culture method for a brassica oleracea L. var. acephala microspore regeneration plant. The method comprises the following steps: taking inflorescences of brassica oleracea L. var. acephala and rape, directly taking or taking after induction alabastrum of the inflorescences, adding the alabastrum into NLN-13 induced medium after disinfection to form fluid suspension, carrying out filtering, and centrifuging the filtrate to obtain a precipitate; adding NLN-13 induced medium and active carbon mixed liquor in order so as to obtain a microspore fluid suspension; carrying out a heat shock treatment, followed by culturing so as to obtain embryoids in cotyledon stage; inoculating the embryoids to embryoid differential medium until the embryoids are differentiated and regenerates into buds; cutting the regenerated buds to a rooting medium for rooting culture, and hardening and transplanting seedlings to obtain regenerated plants; taking young leaves of the regenerated plants for the detection of ploidy of corresponding regenerated plants and determining regenerated seedlings of rape and brassica oleracea L. var. acephala in the regenerated plants. According to the method provided in the invention, rape which is easy to generate embryos and brassica oleracea L. var. acephala which is difficult to generate embryos are mixedly cultured; the material which is easy to generate embryos is used to spur the material which is difficult to generate embryos; therefore the ratio of embryos of brassica oleracea L. var. acephala is improved.

The invention discloses purple cabbage fresh-and-tender pickles and a preparation method thereof. The purple cabbage fresh-and-tender pickles consist of purple cabbage, carrot, garlic sprout, fresh bamboo shoot, young ginger, ripe sesame, momordica grosvenori, bunge cherry seed, semen cassiae, hawthorn, poria cocos, cayenne pepper, black pepper and bean paste. The purple cabbage fresh-and-tender pickles has the beneficial effects that the pickles contain lactic acid bacteria and other substances conductive to good health, has special flavor, is convenient to store, and is deeply loved by people in autumn and winter. Lactic acid bacteria are produced in a fermentation process of the pickles, which facilitates digestion and adjusting of the function of intestines and stomach for the old.

The invention discloses a method for efficiently obtaining a regeneration plant by cultivating isolated microspores of brassica oleracea L.var.capitata L.. The method comprises the following steps: (1) performing embryogenesis; and (2) performing embryo germination, thereby obtaining the regeneration plant, wherein the separated microspores are subjected to dark culture in a culture box at the temperature of 18+/-0.5 DEG C until an embryoid visible to the naked eyes is generated in the step (1) of performing embryogenesis; and then the small embryoid is transferred to a dark shaking table at a speed of 50-55 revolutions per minute at the temperature of 25+/-0.5 DEG C to perform shake culture until the cotyledonous embryoid is formed. According to the method, the genetically homogenous double-haploid plant materials can be obtained in batches, the germplasm resource innovation is accelerated, and the breeding progress is shortened. The method disclosed by the invention can be popularized and applied in the research field of molecular marker assisted breeding, mutant screening and cell totipotency mode systems of the brassica oleracea L.var.capitata L..

The invention discloses a method for rapid propagation of brassica oleracea by a grafting technique. The method includes: selecting appropriate scions and stocks, adopting a bud grafting method for grafting, creating an integral moisture-preserving heat-preserving high-irradiation environment after grafting, managing properly after greening of scion brassica oleracea axillary buds, and finally subjecting scion brassica oleracea to rooting and transplanting. According to the method, brassica oleracea is easy in bolting, grafting to rachises is simpler and more convenient in operation than grafting to dwarf stems, bolts are easy to form lateral bolts, and more stock sources can be provided. By adoption of the bud grafting method, simplicity in operation is achieved, pollution procedures are reduced, and scion survival rate can be increased. White brassica oleracea axillary buds are subjected to strong light exposure after grafting, the white axillary buds can be induced to generate chlorophyll, and accordingly grafting survival rate can be increased. By adoption of tin foil paper for making a simple funnel-shaped rooting device, a moisture preserving environment can be created, induction for rooting is facilitated, and the survival rate is increased.

The invention discloses a vegetable interplanting method which can be implemented all year around. According to the technical scheme, spinach is planted at the beginning of November, Malabar spinach is planted in the middle of November, and spinach and Malabar spinach are interplanted; next year, after spinach is harvested from early March to the middle of March, rape is planted; after Malabar spinach is harvested from early March to the beginning of April, bitter chrysanthemum is planted and rape and bitter chrysanthemum are interplanted; after rape is harvested from the beginning of May to the late May, lettuce is planted; after bitter chrysanthemum is harvested from the beginning of June to the end of July, brassica oleracea is planted and the lettuce and the brassica oleracea are interplanted; after lettuce is harvested from early August to the beginning of September, leaf lettuce is planted, the brassica oleracea is harvested from the early October to late October, and the leaf lettuce is harvested at the beginning of November. According to the method disclosed by the invention, various vegetables can be produced all year round after being combined and interplanted, thus fully utilizing land resources, and having obvious benefits in both production increase and income increase.

The invention discloses a breeding method of wavy Brassica oleracea var. acephala f. tricolor Hort., which comprises the following steps: hybridizing by using asparagus broccoli cytoplast male sterile material CMS034 as a female parent and red curly kale inbred line Ruiyu 6 as a male parent; backcrossing with the original male parent by selecting the individual plant, which has characters closest to the parental characters, from filial generation as the female parent; repeating the second step, and continuously backcrossing to obtain a red curly kale male sterile line; and hybridizing to obtain the wavy Brassica oleracea var. acephala f. tricolor Hort. hybrid by using the red curly kale male sterile line as the female parent and amaranth broad-leaf Brassica oleracea var. acephala f. tricolor Hort inbred line Y305 as the male parent. The new hybrid species obtained by the method disclosed by the invention has the advantages of high ornamental value, long ornamental period, cold resistance and disease resistance.

The invention provides two SSR labels that located on both sides of the brassica oleracea red leaf gene Re and are closely linked to the gene, i.e. C9Z90 and C9Z94. The genetic distances between the two labels and the Re gene are 0.3cM and 2.0cM respectively. According to the nucleotide sequences of the two labels, two pairs of specific amplification primers are designed respectively for molecule assisted selection of brassica oleracea white leaf gene. In the assistant selection process, the two labels can be used at the same time, and have the characteristics of good repeatability, high reliability, low detection cost, and time saving and labor saving.

The invention discloses a method for preserving Brassica oleracea L.var.italicaPlanch. The method comprises the steps of material selection, precooling, rinsing, fresh-cutting, antibacterial coating, sorting, air-conditioning refrigeration and the like, wherein the step of rinsing is that CaCl2 ice water with the concentration of 1-3% is used to rinse for 5-10 min; the step of antibacterial coating is that the fresh-cut Brassica oleracea L.var.italicaPlanch cuts are soaked in natto filtrate with the concentration of 0.1-0.5% for 10 seconds; the framed Brassica oleracea L.var.italicaPlanch is placed in an air-conditioning warehouse for refrigeration, wherein the air-conditioning warehouse is provided with CO2, O2 and N2 and has the humidity of 80-90%; volume percentages of CO2, O2 and N2 are as follows: 1-9% of CO2, 9-1% of O2 and 90% of N2. The method provided by the invention is based on quality characteristics of Brassica oleracea L.var.italicaPlanch, and is reasonable in process design, simple and practical in technical solution, and low in cost, thereby being suitable for industrial production. The preservation period of Brassica oleracea L.var.italicaPlanch is more than 90 days after the rinsing, antibacterial coating, air-conditioning refrigeration and other comprehensive matching preservation technologies are implemented.