96 results about "Brassica sp." patented technology

The invention discloses a brassica plant drought resistance negative regulation factor BnaA9.TZF5 and application of the brassica plant drought resistance negative regulation factor BnaA9.TZF5. The BnaA9.TZF5 is a transcription factor gene for coding CCCH type zinc finger protein in brassica napus, and a knockout plant obtained by performing targeted editing on the BnaA9.TZF5 through CRISPR / Cas9 shows that the drought resistance is remarkably enhanced along with phenotypic changes such as increase of leaf epidermis wax deposition and reduction of water loss rate; and an overexpression plant of the gene shows that the wax is reduced, the water loss is accelerated and the drought resistance is weakened, so that the BnaA9.TZF5 is a negative regulation factor for the drought resistance of the brassica plant. The BnaA9.TZF5 gene expression is knocked out to improve the drought resistance of brassica plants, the BnaA9.TZF5 gene can be used for molecular breeding of crops such as brassica napus, Chinese cabbage, cabbage and mustard, and a novel drought-resistant improved target gene is provided.

The present invention relates to Brassica oleracea plants resistant to the plant pest diamondback moth Plutella xylostella and, additionally, tolerant to the plant pathogen Xanthomonas campestris pv. campestris. The present invention further relates to methods for identifying the present plants and nucleic acid sequences allowing characterizing, or identifying, the present plants. Specifically, the present invention relates to Brassica oleracea plants resistant to the diamondback moth Plutella xylostella and the plant pathogen Xanthomonas campestris campestris, wherein said resistance to the diamondback moth Plutella xylostella and said tolerance to the plant pathogen Xanthomonas campestris campestris are obtainable from a Brassica oleracea plant deposited under deposit number NCIMB 43822.

Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic / transgene junctions within SEQ ID NO: 2 or with genomic / transgene junctions as set forth in SEQ ID NO: 12 and / or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

The application discloses a molecular marker for distinguishing early and late flowering of Brassica oleracea and application thereof, wherein the nucleotide sequence of the molecular marker exists in the non-coding region of chromosome 4 of the genome of early flowering Brassica oleracea, and does not exist in the non-coding region of chromosome 4 of the genome of late flowering Brassica oleracea; based on the molecular marker, a primer pair Bf5 is further designed, DNA of the Brassica oleracea is amplified by using the primer pair, and early flowering varieties or materials of the Brassica oleracea can be screened according to the presence or absence of the target band. The molecular marker and the primer pair provided by the application can be used for rapidly detecting early flowering varieties and materials of the Brassica oleracea, time and resources are greatly saved, selection efficiency is greatly improved, and thus the breeding process is accelerated.

The invention discloses a chitosan modified turmeric-broccoli exosome hybrid nasal spray and application thereof, and belongs to the technical field of biological medicine and drug delivery. The nasal spray is formed by dispersing double plant exosome-lipidosome hybrids in a carrier for nasal cavities. The preparation method comprises the following steps: fusing curcumin carried by a turmeric exosome and sulforaphane carried by a broccoli bud exosome with a pH response liposome membrane containing 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine, and modifying by chitosan to form a three-layer structure; the preparation method comprises the steps of exosome extraction, ultrasonic drug loading, liposome hybridization, chitosan coating and preparation blending. The nasal spray is high in entrapment efficiency and uniform in particle size, the chitosan enhances nasal adhesion, inflammation targeted drug release is achieved through pH response, the brain targeting efficiency and stability are excellent, and the adaptability to children is good. The curcumin and sulforaphane are used for targeted therapy of children autism spectrum disorder neuroinflammation, anti-inflammation and anti-oxidation are achieved through synergism of curcumin and sulforaphane, the curative effect is remarkable, and a new scheme is provided for treatment of related diseases.

The invention provides compositions and methods relating to the elevation of glucoraphanin compared to standard Brassica oleracea varieties. The invention also relates to the production of hybrid varieties having desired glucosinolate contents. The invention further provides plants, plant parts, and seeds comprising such traits and comprising a Myb28 allele from Brassica villosa that is not genetically linked to an ELONG allele from Brassica villosa.

The invention discloses a primer group for identifying a broccoli nuclear male sterility gene and application of a molecular marker, and belongs to the technical field of biology. The primer group comprises a first primer pair and a second primer pair, each primer pair comprises a forward primer and a reverse primer, and the sequences of the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the second primer pair and the reverse primer of the second primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 4 in a sequence table. By applying the primer group and the molecular marker, the fertility of the broccoli can be efficiently and accurately identified, the problem that 50% of fertile plants need to be pulled out in seed production is solved, and the manpower and material resource cost is reduced.

The present invention relates to a plant material for use as medicament, wherein the plant material is derived from a plant of the Brassica, Spinacia, or Lactuca genus, genetically modified to express a biologically active recombinant protein and comprises the biologically active recombinant protein as an active ingredient. The invention also relates to a method for producing such a plant material, and to a pharmaceutical composition comprising such a plant material and an orally acceptable support.

The invention relates to a process for preparing an article comprising:i) providing a two-part adhesive composition having a liquid part comprising an amine-based azetidinium-functional cross-linker, a diluent and water, and a solid part comprising ground Helianthus meal or ground Brassica meal or a mixture thereof, having a granulometry d50 of less than 300 μm,ii) adding either the liquid part or the solid part to a lignocellulosic material to provide a mixture, theniii) adding the remaining liquid or solid part to the mixture to provide a lignocellulosic material impregnated with the adhesive composition.

The present invention relates to clubroot resistance in Brassica. More specifically, the invention relates to the CRT clubroot resistance gene, as well as to methods to create clubroot resistant Brassica plants.

A thermochromic ink and, particularly, to a thermochromic ink produced by combining an anthocyanidin extract with alginate. The thermochromic ink can be used for authentication purposes produced by combining an anthocyanidin (ACYD)-based Brassica oleracea L. var. capitata extract with an alginate (ALG). To increase the anthocyanidin / alginate ink stability, a mordant (ferrous sulfate) can be used to link the anthocyanidin biomolecules with the alginate.

The application belongs to the technical field of molecular breeding, and particularly relates to a SCAR marker for detecting a heterologous radish mitochondrial fragment in a Brassica oleracea Ogura cytoplasmic male sterile line. The application provides a SCAR marker for detecting a heterologous radish mitochondrial fragment in a Brassica oleracea Ogura cytoplasmic male sterile line, which comprises one or more or all of markers AC1-AC16; the nucleotide sequences of the AC1-AC16 are shown in SEQ ID NO: 1-16. The SCAR marker of the application has high specificity and good stability, and can accurately identify whether the corresponding heterologous radish mitochondrial fragment is contained in a to-be-tested Brassica oleracea cytoplasmic male sterile line and the relative content thereof, thereby being beneficial to early selection of the Brassica oleracea cytoplasmic male sterile line, improving breeding efficiency, and reducing breeding cost.

The invention belongs to the technical field of microorganisms, and discloses application of verticillium lecanii in promoting cabbage growth. The verticillium lecanii JMC-01 (the preservation number is CCTCC NO: M2018303) is successfully colonized in a cabbage plant through spray inoculation, the invention further proves that the endophytic colonization of the verticillium lecanii JMC-01 has an obvious improvement effect on agronomic characters such as the plant height and the root length of the cabbage, and the verticillium lecanii JMC-01 has a good application prospect in the aspects of promoting the growth of the cabbage plant and increasing the yield of the cabbage.

This disclosure provides methods and compositions for identifying Brassica plants that have a native deletion of the INDEHISCENT gene (BnIND-A) located on chromosome A of B. napus. Also provided are methods of improving one or more agronomic characteristics such as pod shatter and breeding methods for introducing a pod shatter tolerant phenotype in Brassica plants and / or their progeny.

1. The name of the design product: packing bag (Brassica oleracea garlic hawthorn drink). 2. The use of the design product: a packing bag of a product. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: front view. 5. The design product is a thin product, and other views are omitted.

The invention provides an RPA (recombinase polymerase amplification) primer group, a kit and a method for detecting the alternaria brassicae of broccoli, which are used for detecting the alternaria brassicae of broccoli. The primer group comprises specific primers F1 / R1, F1 / R1 and F2 / R1, and the sizes of theoretical amplification products of the primer group are 204bp, 187bp and 189bp. Specific target genes are screened out through comparative genomics, RPA primers are designed, and a constant-temperature (39 DEG C) and rapid (20 minutes) detection method is established. The primer provided by the invention has strong specificity, is only effective to the brassica oleracea var. Brassicae, and has no cross reaction with sibling species; the sensitivity is high, the detection lower limit is 0.08 ng / mu L, and the sensitivity is 100 times higher than that of conventional PCR. The actual sample verifies that the detection accuracy reaches 100%. The method is easy and convenient to operate, rapid in detection and suitable for field early diagnosis, plant quarantine and disease monitoring of the pathogenic bacteria.

Methods are provided to mutate, in a targeted manner, the genome of a plant cell using a double stranded DNA break inducing enzyme. Also provided are plants, in particular Brassica plants that yield seeds producing oils having a reduced total saturated fatty acid content, and method for making such plants.

The invention discloses a method for high-yield cultivation of oxheart cabbages by using mountain tea garden conditions, relates to the technical field of cabbage planting, and aims to solve the problems of low resource utilization rate of northern-Sichuan mountain special tea gardens, poor cultivation suitability of spring-stubble oxheart cabbages and insufficient comprehensive benefits in the prior art. By combining the growth characteristics that the white-leaf No.1 tea tree variety and the middle-yellow No.1 tea tree variety grow slowly and grow weakly (plants are relatively short) in the North Sichuan tea area relative to general green tea and the actual construction of the white-leaf No.1 tea garden and the middle-yellow No.1 tea garden, the high-yield cultivation system of the oxheart cabbage adaptive to the white-leaf No.1 tea garden and the middle-yellow No.1 tea garden is constructed, and multiple beneficial effects are achieved. According to the method, the growth rhythm of'spring harvesting and summer cultivation 'of tea trees is accurately matched, relay intercropping is conducted after spring tea is harvested and pruned, the influence on spring tea core output is completely avoided, and the tea garden land and the light energy utilization rate are greatly improved.

The present disclosure provides Brassica oleracea plants having curds or heads exhibiting increased resistance to downy mildew. Such plants may comprise novel introgressed genomic regions associated with disease resistance from Brassica oleracea MYCOCLP. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.

The utility model discloses kale wall breaking and juicing equipment, and relates to the field of wall breaking machines, the kale wall breaking and juicing equipment comprises a wall breaking machine cup body, a wall breaking cylinder, a buckling cover and a cooling mechanism, an electric cutter is arranged on a base of the wall breaking machine cup body, the cutter, the wall breaking cylinder and the cooling mechanism form a wall breaking bubble removing assembly, and a filter cartridge is arranged on the wall breaking machine cup body. A bubble removing sharp block is installed on the inner wall of the filter cylinder, the increase of the temperature of liquid during juicing can be reduced through the wall breaking bubble removing assembly, then generation of bubbles is reduced, after juicing is finished, the wall breaking cylinder is dismantled to enable the liquid to enter the filter residue collecting chamber, the liquid is filtered through the filter cylinder, the bubbles moving to the filter screen are removed through the bubble removing sharp block, and therefore the juicing effect is improved. Filter residues and generated bubbles are left in the filter residue collecting chamber and cannot flow to the outside, so that the taste of liquid is ensured, and the product quality is improved.

The invention relates to the field of vegetable processing equipment, in particular to an automatic conveying device for raw materials. The automatic conveying device for the raw materials comprises a mounting bottom plate, a mounting frame is connected to the mounting bottom plate, a driving roller and a driven roller are rotationally connected to the mounting frame, a crusher is mounted on the mounting frame, the automatic conveying device further comprises chains and the like, and the chains are connected to the left side and the right side of a conveying belt. A placing mechanism used for placing raw materials is arranged on the chain, a pushing mechanism used for pushing the raw materials to ascend is arranged on the mounting bottom plate, and a clamping assembly used for fixing the raw materials is arranged on the placing mechanism. Brassica oleracea is jacked up through the air cylinder, the multiple brassica oleracea is fixed through the clamping assembly at the same time, then stem cores of the multiple brassica oleracea are removed through the crusher, therefore, in the brassica oleracea conveying process, the stem cores of the brassica oleracea are crushed, the purpose of automatically removing the stem cores of the brassica oleracea is achieved, the brassica oleracea does not need to be held by hands, potential safety hazards are eradicated, the stem cores of the multiple brassica oleracea are removed at the same time, and the stem core removing efficiency is improved

The present invention relates to a composition based on ethanolic extract of Brassica olearacea var. capitata leaves, anhydrous lanolin and castor oil, for the treatment of burns and their complications, such as infections. Emulsions made from ethanolic extract of Brassica olearacea var. capitata leaves, anhydrous lanolin and castor oil in semi-solid formulations such as creams and ointments, with 10% and 20% w / v of Brassica extract, can be used as healing agents, accelerating closure of the skin area damaged by up to 3rd degree burns, inducing the production of protective enzymes as well as the proliferation of cells and blood vessels, modulating the production of matrix components and stimulating the formation of strong scar tissue, while having no side effects due to their lack of toxicity.

The invention belongs to the technical field of brassica plant breeding, and relates to a reagent and method for overcoming self-incompatibility of Chinese cabbage and application. After the Chinese cabbages enter the flowering phase, an EGCG (epigallocatechin gallate) solution is sprayed on the stigmas of the Chinese cabbages, so that the self-incompatibility of the Chinese cabbages can be weakened to different degrees. Experimental results show that after the EGCG is sprayed, the number of seeds obtained from each pod of the self-incompatible Chinese cabbage is increased to 17.8 from original 0. The used reagent EGCG does not pollute the environment, especially the soil environment, has small damage to plants, and can be applied to seed reproduction work of the Chinese cabbage self-incompatible line.

The invention relates to the technical field of plant genetic breeding, in particular to a brassica napobrassica fatty acyl reductase BcFAR2 gene and expression protein and application thereof, the base sequence of the brassica napobrassica fatty acyl reductase BcFAR2 gene is shown as SEQ ID NO.1, and the amino acid sequence of the expression protein is shown as SEQ ID NO.2. The brassica napobrassica fatty acyl reductase BcFAR2 gene is cloned, and the brassica napobrassica fatty acyl reductase BcFAR2 gene can be used for preparing the brassica napobrassica fatty acyl reductase BcFAR2 gene. The BcFAR2 gene overexpresses a black cabbage sterile plant, so that the pollen activity can be recovered; the pollen activity of the gene knockout black cabbage fertile plant can be obviously reduced; the gene is over-expressed in arabidopsis thaliana, and it is found that the salt tolerance of a transgenic plant can be remarkably improved; the gene is over-expressed in black cabbage, so that the salt tolerance is obviously enhanced.

The invention relates to the use of a lytic bacteriophage, stabilised in a carrier, of the family Myoviridae, Foxunavirus genus (deposit IDAC 261122-01), which acts as a biocontroller of Xanthomonas campestris pv. Campestris (Xcc), a phytopathogen responsible for the main disease affecting brassicas globally, angular leaf spot or black rot. In addition, the invention relates to the preparation of a bioproduct comprising the phage and an agronomically acceptable carrier, such as alginate and / or chitosan.

This invention provides a genetically engineered *Yersinia lipolytica* strain and its applications. The genetically engineered strain is a *Yersinia lipolytica* Po1f strain with the PEX10 gene knocked out, expressing the C16 / 18 elongase gene MaELO3 from *Morchella alpina*, the elongase gene AtKCS from *Arabidopsis thaliana*, and the elongase gene CraKCS from *Brassica oleracea*. The strain also expresses the elongase gene CgKCS from *Capsella*. Further expression of the desaturase gene MaD15D from *Morchella alpina*, and overexpression of the strain's own diacylglycerol transferase gene DGA1 and its own Δ9 desaturase gene OLE1, resulted in a nervonic acid yield of 111.6 mg / L in shake-flask fermentation. By adding 1% rapeseed oil exogenously, the nervonic acid yield reached 185.1 mg / L. The *Yersinia lipolytica* genetically engineered strain constructed in this invention is simple to operate, has stable and reliable performance, and can be applied to large-scale commercial production.

Fish feeds that contain omega-3 fatty acids derived from modified oilseed plants such as Brassica and others, in some cases in place of or in addition to omega-3 fatty acids derived from marine oil.

This invention discloses a recessive nuclear male sterility gene. BoaGMS1 Application in regulating male development in Chinese kale. This invention screens recessive nuclear male sterility mutants from Chinese kale breeding and isolates the Chinese kale male fertility gene using forward genetics. BoaGMS1 Further experiments confirmed that BoaGMS1 A gene mutation resulted in recessive nuclear male sterility in Chinese kale. Meanwhile, targeting... BoaGMS1 Cosegregating molecular markers developed for male-sterile lines can be used for identifying fertility alleles in plants, screening target plants in marker-assisted breeding, and determining seed purity. Furthermore, they can be used in the development of kale. BoaGMS1 Male-sterile lines can be applied to the breeding and production of new hybrid varieties of Chinese kale. Therefore, the male fertility gene for Chinese kale provided by this invention... BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS It plays an important role in the utilization of heterosis and hybrid seed production of Chinese kale and even Brassica oleracea vegetables.

System for the development of a pH-based biosensor using anthocyanins from red cabbage (Brassica oleracea) and chitosan nanoparticles for monitoring food quality, comprising: ◯ Glassware for collecting and processing red cabbage samples, using a system for extracting anthocyanins by: • Thoroughly wash red cabbage with tap water and distilled water; • Chop the washed cabbage into fine pieces of about 1-2 cm; • Soaking 2 kg of shredded cabbage in 100 ml of acidified ethanol solution (ethanol:water:acetic acid, 70:29:1 v / v / v) for 24 hours; • Filter the extract using a vacuum filter and concentrate it under reduced pressure; • Store the extract at 4°C in a dark bottle; ◯ a device for the synthesis of chitosan nanoparticles via ionic gelation, comprising: • Prepare a 0.1% (w / v) chitosan solution in 1% acetic acid, adjusted to pH 4.6; • Add 0.1% tripolyphosphate (TPP) solution dropwise while stirring; • Collecting the nanoparticles by centrifugation and washing with deionized water; ◯ a system for introducing anthocyanins into chitosan nanoparticles by mixing the anthocyanin extract with the chitosan solution prior to TPP addition; ◯ a solution casting system for the production of a pH-sensitive biofilm by mixing anthocyanin-loaded nanoparticles with poly(vinyl alcohol); ◯ analytical instruments, including an Alpha E ATR-FTIR (Bruker, Germany) and an FEI QUANTA 450 scanning electron microscope, for characterizing the nanoparticles and biofilm; ◯ Design of a well diffusion test to assess antibacterial activity against Staphylococcus aureus using Mueller-Hinton agar plates.

System for the production of silver nanoparticles using red cabbage (Brassica oleracea), consisting of: a glass device used to collect red cabbage (Brassica oleracea) samples from a vegetable market, wherein the system is used to perform a synthesis of silver nanoparticles using the collected red cabbage samples, comprising the following steps: Thoroughly wash the collected red cabbage with tap water and distilled water; Cut the washed red cabbage into pieces about 1-2 cm in size; Extracting the weighed 2 kg of chopped red cabbage in 100 ml of hot distilled water using a microwave oven with a microwave power of 900 W for 120 seconds; Filtering the aqueous solution extract through a vacuum filter and storing the extracted red cabbage at 20 °C for further analyses and experiments; a dark brown bottle, wherein AgNO3 is used as a silver ion complex to produce Ag-NPs, so that the AgNO3 is produced in the dark and stored in the dark brown bottle; the AgNO3 solution is mixed with the extracted red cabbage and the mixed solution is left in the dark for one day; three Erlenmeyer flasks, into which 90 ml AgNO3 and 10 ml distilled water are added, then 90 ml AgNO3 with 10 ml red cabbage extract are added and the 90 ml distilled water with 10 ml red cabbage extract are added to the three Erlenmeyer flasks; the three Erlenmeyer flasks are held in a water bath to provide heat and to support the formation of Ag-NPs the following day.