Method of improved artificially synthesized Brassica napa L. for heterosis utilization

A technology of artificial synthesis of Brassica napus, applied in horticultural methods, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of artificially synthesized Brassica napus of low quality, etc., to improve the potential of heterosis and improve the low quality Effect

Inactive Publication Date: 2010-07-28
SOUTHWEST UNIVERSITY
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  • Abstract
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the disadvantages of poor quality of the existing artificially synthesized Brassica napus, and provide a method for improving artificially synthesized Brassica napus for heterosis utilization, thereby improving the potential of artificially synthesized Brassica napus for heterosis utilization

Method used

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Embodiment Construction

[0016] The following is a specific implementation of the method of the present invention, but it is not a limitation of the method of the present invention. Any transformation that does not depart from the essence of the present invention should still belong to the protection scope of the present invention.

[0017] 1. Breeding of new oily cabbage

[0018] Taking Brassica napus SWU-1 (the content of glucosinolate in the seed cake is 20 μmol / g, and the content of erucic acid in the seeds is less than 0.5%) as the female parent, and Brassica napus C-1 as the male parent, the female parent was peeled and emasculated, and there were Sexual hybridization, 8-12 days after pollination, interspecific hybrid test-tube plantlets are obtained by embryo rescue. Then the interspecific hybrid test-tube plantlets were transplanted to the field, and at the flowering stage, artificially detasselled and inoculated with cabbage pollen, and BCF was obtained by embryo rescue. 1 , or microspore cu...

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Abstract

The invention discloses a method of improved artificially synthesized Brassica napa L. for heterosis utilization, belonging to the technical field of breeding plant novel varieties. The method comprises the following steps: transferring the excellent oil economical characters (the content of thioglycoside in seed cake dregs is lower than 30 mu mol/g, and the content of erucic acid in seeds is lower than 1% of double-low Brassica napa L.) of the double-low Brassica napa L. to wild cabbage; culturing a novel oil wild cabbage material, hybridizing the material with a double-low Brassica campestris variety to obtain the artificially synthesized Brassica napa L. with normal fecundity, wherein, the content of thioglycoside in seed cake dregs is lower than 30 mu mol/g, the content of erucic acidin seeds is lower than 1%, and the superior combination prepared by the artificially synthesized Brassica napa L. and the natural Brassica napa L. is screened. In the invention, the good genes of Brassica napa L. are transferred to wild cabbage to culture oil wild cabbage material which is for overcoming the disadvantages of low quality and low fecundity of the existing artificially synthesized Brassica napa L., thereby improving the potential of artificially synthesized Brassica napa L. for heterosis utilization.

Description

technical field [0001] The invention belongs to the technical field of new plant strain breeding, and specifically relates to a method for improving artificial synthesis of Brassica napus for heterosis utilization. Its international patent classification number is A01H1 / 04. Background technique [0002] Brassica napus (AACC) is an allotetraploid (U 1935) evolved from the natural hybridization of Brassica napus (B.rapa, AA) and Brassica napus (B.oleracea, CC) through chromosome doubling. Brassica napus has a narrow genetic base compared to the parent species, which limits the genetic improvement of existing Brassica napus. By crossing Brassica napus and Brassica napus, synthetic Brassica napus can be used to broaden the genetic base of existing Brassica napus, and the potential of heterosis utilization (Seyis et al. 2003, Plant Breeding 122: 473-478). [0003] However, in the existing cabbage resources, there is no oily cabbage, which makes the artificially synthesized Bras...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/08A01H4/00
Inventor 钱伟李加纳马丁·弗奥梅家勤李勤菲殷家明
Owner SOUTHWEST UNIVERSITY
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