Method for identifying celery cabbage-brassica oleracea alien addition lines

A technology of hetero-additional line and Chinese cabbage, which is applied in the field of molecular genetics theory application and breeding, can solve the problem of not finding the method of Chinese cabbage-cabbage hetero-additional line, etc., and achieves the effects of improving accuracy and good communication.

Inactive Publication Date: 2009-05-06
HEBEI AGRICULTURAL UNIV.
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Problems solved by technology

After searching, no ideal method for identification of Chinese

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  • Method for identifying celery cabbage-brassica oleracea alien addition lines
  • Method for identifying celery cabbage-brassica oleracea alien addition lines
  • Method for identifying celery cabbage-brassica oleracea alien addition lines

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Embodiment Construction

[0027] The following examples illustrate the invention.

[0028] The embodiment obtains the allotriploid hybrid with Chinese cabbage-cabbage double monomer different addition line (tetraploid Chinese cabbage crosses with diploid cabbage, and the hybrid is backcrossed with diploid Chinese cabbage, and in the backcross population The identification of the screened 2n=20A+2C=22) is taken as an example to illustrate the present invention.

[0029]Explanation of the principle: Using the specific SSR primers of the cabbage linkage group relative to Chinese cabbage, the genomic DNA of hybrids and their parents Chinese cabbage, cabbage, and isoadditional lines were used as templates for PCR amplification, and their amplification products were compared. The amplified products of the specific primers in the heteroaddition line are consistent with those of Chinese cabbage, indicating that there is no such linkage group of cabbage in the heteroaddition line. If the amplification products ...

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Abstract

The invention discloses a method for identifying Chinese cabbage-cabbage addition lines. The method comprises the following steps: (1) selecting 91 pairs of primers in different linkage groups of cabbage according to a published cabbage genetic map; (2) extracting genetic groups DNA of different Chinese cabbages and cabbages to carry out PCR amplification; (3) electrophoretically separating an amplified product in modified polyacrylamide gel of 6 percent to carry out silver staining; (4) screening SSR primers in the linkage groups of the cabbage different from the Chinese cabbage; and (5) identifying the Chinese cabbage-cabbage addition lines by the SSR primers in the linkage groups of the cabbage different from the Chinese cabbage. The method has the advantages that the method can quickly and accurately identify important genetic analysis materials for the Chinese cabbage and the cabbage, such as coenospecies, addition lines, alien substitution lines, translocation lines and the like on molecular level, and lays foundation for modifying Chinese cabbage genetic background by cabbage gene.

Description

technical field [0001] The invention relates to a method for identifying Chinese cabbage-cabbage isoaddition lines, and belongs to the technical field of molecular genetics theory application and breeding. Background technique [0002] At present, the identification of chromosomes in Chinese cabbage (Brassica campestris syn rapa ssp. Pekinensis, AA) and cabbage (B. oleracea var. L., CC) is limited to the cellular level. Due to the small size and similar shape of the chromosomes, the method of karyotype analysis is difficult. Accurate identification of Brassica oleracea chromosomes in the context of Chinese cabbage chromosomes. Chen et al. used Brassica campestris-B.oleracea var. alboglabra monosomy heterologous extra-synaptic chromosome karyotype analysis of root tip somatic cells, and only identified one heterologous extra-synaptic line with the longest chromosome in cabbage; Genomic in situ hybridization cannot distinguish between the two chromosomes; Robert et al. used f...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 申书兴顾爱侠王彦华陈雪平赵建军
Owner HEBEI AGRICULTURAL UNIV.
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