Culture method for brassica oleracea L. var. acephala microspore regeneration plant

Abstract

The invention discloses a culture method for a brassica oleracea L. var. acephala microspore regeneration plant. The method comprises the following steps: taking inflorescences of brassica oleracea L. var. acephala and rape, directly taking or taking after induction alabastrum of the inflorescences, adding the alabastrum into NLN-13 induced medium after disinfection to form fluid suspension, carrying out filtering, and centrifuging the filtrate to obtain a precipitate; adding NLN-13 induced medium and active carbon mixed liquor in order so as to obtain a microspore fluid suspension; carrying out a heat shock treatment, followed by culturing so as to obtain embryoids in cotyledon stage; inoculating the embryoids to embryoid differential medium until the embryoids are differentiated and regenerates into buds; cutting the regenerated buds to a rooting medium for rooting culture, and hardening and transplanting seedlings to obtain regenerated plants; taking young leaves of the regenerated plants for the detection of ploidy of corresponding regenerated plants and determining regenerated seedlings of rape and brassica oleracea L. var. acephala in the regenerated plants. According to the method provided in the invention, rape which is easy to generate embryos and brassica oleracea L. var. acephala which is difficult to generate embryos are mixedly cultured; the material which is easy to generate embryos is used to spur the material which is difficult to generate embryos; therefore the ratio of embryos of brassica oleracea L. var. acephala is improved.

Description

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AI Technical Summary

Benefits of technology

This patented new technique allows for easier creation of hybrid crops with different types of vegetables such as carrots or cauliflower flowers by mixing them together without damaging their growth. By doing this it increases the yield from these plants compared to traditional methods like crop rotation.

Problems solved by technology

This patented describes various technical solutions aimed towards increasing the speed and yield of creation of edible crop species called brassic acid tamarina varies by growing them over several generations or developing new ones through crossing techniques such as seedling propagating technique. However, these conventional ways require significant labor input during each generation cycle due to their slow growth rates compared to natural occurrences found throughout this world's tropics. To address this issue, we propose a novel way to increase the germinancy rate of certain types of brassiercane grown commercially without requiring excessive human effort.

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